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Full-Text Articles in Dairy Science
Genetic Determinants Associated With Persistence Of Listeria Species And Background Microflora From A Dairy Processing Environment, Vaishali Poswal, Sanjeev Anand, Jose L. Gonzalez-Hernandez, Brian Kraus
Genetic Determinants Associated With Persistence Of Listeria Species And Background Microflora From A Dairy Processing Environment, Vaishali Poswal, Sanjeev Anand, Jose L. Gonzalez-Hernandez, Brian Kraus
Dairy Science and Food Science Faculty Publications: 2022 to Present
Listeria monocytogenes is a persistent foodborne pathogen capable of surviving in food processing environments, often in association with diverse environmental microflora. This study examines genomic determinants of persistence, specifically stress adaptation and biofilm-associated traits, in environmental Listeria species and other environmental microflora from a dairy processing facility by analyzing whole-genome sequences of 6 environmental Listeria isolates, 4 ATCC reference strains, and 22 air and floor swab cultures, annotated using the RAST platform. Subsystem analysis revealed that Listeria isolates carried a defined set of genes linked to biofilm formation, antimicrobial resistance, and stress response, though in lower abundance than environmental cultures. …
Enhancing The Biopreservative Effect Of Non-Starter Lactic Acid Bacteria Using Soluble Fiber During Cheese Ripening, Rakesh Kaushik, Sanjeev Anand
Enhancing The Biopreservative Effect Of Non-Starter Lactic Acid Bacteria Using Soluble Fiber During Cheese Ripening, Rakesh Kaushik, Sanjeev Anand
Dairy Science and Food Science Faculty Publications: 2022 to Present
Cheddar cheese ripening creates favorable conditions for desired microbial changes but also allows survival and outgrowth of spores like Clostridium tyrobutyricum and Bacillus licheniformis, leading to late-blowing defects. In the first phase of the study, NSLAB dynamics were evaluated in the presence of spores, where pilot-scale cheeses (110 L) were produced in four treatments: control, T1 (BL), T2 (CT), and T3 (BL+CT), each inoculated at 2.0 Log10 CFU/mL with spores. Results showed that SLAB declined from 8.0 to 0.2 Log10 CFU/g, while NSLAB increased from 2.0 to 8.5 Log10 CFU/g by the third month and remained stable thereafter. Spore counts …
Using Automated Activity Monitoring To Detect Resumption Of Cyclicity In Early Lactation—Meta-Analysis, S. Borchardt, T.A. Burnett, T.C. Bruinjé, A.M.L. Madureira
Using Automated Activity Monitoring To Detect Resumption Of Cyclicity In Early Lactation—Meta-Analysis, S. Borchardt, T.A. Burnett, T.C. Bruinjé, A.M.L. Madureira
Dairy Science and Food Science Faculty Publications: 2022 to Present
This meta-analysis aimed to evaluate the efficacy of automated activity monitoring (AAM) in detecting estrous expression and ovulatory status in cows during the voluntary waiting period (VWP). A comprehensive literature search was conducted in PubMed, ScienceDirect, and Google Scholar using specific search terms. Inclusion criteria focused on studies that assessed estrous expression within the VWP using modern AAM systems alongside blood progesterone (P4) measurements. Four manuscripts involving 2,198 cows were included. Data extraction was performed by a single investigator and validated by a coauthor. The analysis considered estrous expression and ovulatory status determined through serial blood P4 measurements. Cows were …
Evaluating The Effectiveness Of Individual Cleaning Steps Of A Cip Protocol In Membrane Biofilm Removal Under Dynamic Conditions, Diwaker Singh, Sanjeev Anand
Evaluating The Effectiveness Of Individual Cleaning Steps Of A Cip Protocol In Membrane Biofilm Removal Under Dynamic Conditions, Diwaker Singh, Sanjeev Anand
Dairy Science and Food Science Faculty Publications: 2022 to Present
This study evaluated the effectiveness of individual clean-in-place (CIP) steps in removing biofilms from reverse osmosis (RO) membranes under dynamic flow conditions using the Centers for Disease Control (CDC) biofilm reactor. Biofilms were developed in the laboratory under continuous flow, using mixed-species bacterial isolates obtained from 10-month-old RO membrane biofilms from a commercial facility. Individual CIP chemicals, representative of those used in commercial protocols, were tested against 24 h-old biofilms. Additionally, a complete six-step sequential CIP process was conducted under dynamic conditions, consisting of treatments with alkali, surfactant, acid, enzyme, a secondary surfactant, and sanitizer. All experiments were performed in …
Studying The Population Dynamics Of Nslab And Their Influence On Spores During Cheese Ripening, Rakesh Kaushik, Sanjeev Anand
Studying The Population Dynamics Of Nslab And Their Influence On Spores During Cheese Ripening, Rakesh Kaushik, Sanjeev Anand
Dairy Science and Food Science Faculty Publications: 2022 to Present
Cheese ripening involves microbial changes, with starter lactic acid bacteria (SLAB) initiat- ing fermentation and nonstarter lactic acid bacteria (NSLAB) driving flavor and texture development. However, heat-resistant spores of Clostridium and Bacillus can survive pas- teurization and cause spoilage during ripening. This study evaluated NSLAB dynamics in the presence of spores during cheese ripening. Cheddar cheese samples at pilot-scale level (110 L) with four treatments, namely control, with spores of B. licheniformis (T1), with spores of Cl. tyrobutyricum (T2), and both spores (T3) at 2.0 Log10 CFU/mL, were ripened at 7 ◦C for six months. SLAB declined from 8.0 to …
Characterizing Environmental Background Microflora And Assessing Their Influence On Listeria Persistence In Dairy Processing Environment, Vaishali Poswal, Sanjeev Anand, Brian Kraus
Characterizing Environmental Background Microflora And Assessing Their Influence On Listeria Persistence In Dairy Processing Environment, Vaishali Poswal, Sanjeev Anand, Brian Kraus
Dairy Science and Food Science Faculty Publications: 2022 to Present
Listeria monocytogenes (Lm) may persist in food processing environments (FPEs) alongside diverse background microflora. While microbial communities in FPEs can in- fluence Lm survival, their role in supporting or suppressing its growth remains unclear. This study aimed to characterize the microflora in floor swabs and air samples collected from a dairy processing facility across three seasons and assess their potential impact on the growth of a Lm test strain previously isolated from a dairy processing environment. A total of 167 environmental isolates, representing 30 bacterial genera, were identified. Pseudomonas was consistently prevalent across all sample types. Seasonal shifts in bacterial …
Potential Of Anogenital Distance As A Genetic Selection Trait In Canadian Holstein, G.R. Dodd, F.S. Schenkel, F. Miglior, T.C. Bruinjé, M. Gobikrushanth, J.E. Carrelli, M. Oba, D.J. Ambrose, C.F. Baes
Potential Of Anogenital Distance As A Genetic Selection Trait In Canadian Holstein, G.R. Dodd, F.S. Schenkel, F. Miglior, T.C. Bruinjé, M. Gobikrushanth, J.E. Carrelli, M. Oba, D.J. Ambrose, C.F. Baes
Dairy Science and Food Science Faculty Publications: 2022 to Present
Maintaining optimal fertility in dairy cattle herds is a global challenge that is typically addressed through the genetic selection of fertility indicator traits. However, many of the traits currently implemented in breeding programs are heavily influenced by environmental factors, resulting in a slow rate of genetic improvement. Anogenital distance (AGD) has recently emerged as a promising fertility indicator trait due to its association with favorable reproductive outcomes and its higher heritability estimates compared with currently evaluated traits. This study aimed to enhance the understanding of AGD's genetic potential by estimating its genetic parameters in Canadian Holsteins, assessing the reliability …
From Block To Shred: Understanding The Factors Influencing Shreddability Of Mozzarella Cheese, Gunvantsinh Rathod, Suresh Saatariya, Ram Kumar, Praveen Upreti, Prafulla Salunke, J.K. Amamcharla
From Block To Shred: Understanding The Factors Influencing Shreddability Of Mozzarella Cheese, Gunvantsinh Rathod, Suresh Saatariya, Ram Kumar, Praveen Upreti, Prafulla Salunke, J.K. Amamcharla
Dairy Science and Food Science Faculty Publications: 2022 to Present
Mozzarella cheese (MC) is widely used for pizza ap- plications, and it is generally shredded either in conver- sion plants or in pizzerias. The shreddability of MC is influenced by a variety of factors and it is critical to understand how different Mozzarella cheese types and storage conditions (temperature and age) affect this prop- erty. Three batches each of 3 types of MC (low-moisture Mozzarella (LMM), low-moisture part-skim Mozzarella (LMPS), and reduced fat Mozzarella (RFM)) representing 3 different fat levels were procured directly from a com- mercial manufacturer and stored at 2 different tempera- tures (1.67 and 4.44°C) and evaluated …
In-Line Milk Progesterone Monitoring As A Tool For Precision Reproductive Management, Tony C. Bruinjé, Divakar J. Ambrose, Stephen J. Lebalnc
In-Line Milk Progesterone Monitoring As A Tool For Precision Reproductive Management, Tony C. Bruinjé, Divakar J. Ambrose, Stephen J. Lebalnc
Dairy Science and Food Science Faculty Publications: 2022 to Present
In-line milk progesterone (IMP4) monitoring (Herd Navigator, DeLaval) is a technology that automatically detects onset of cyclicity, estrus, and pregnancy. Sampling starts at 20 DIM and occurs on average every 2 d until pregnancy. Estrus is detected based on a decline in progesterone (P4) concentrations below a threshold, and pregnancy is assessed from 30 to 55 d after AI in cows without return to estrus. Here, we review the potential of IMP4 as a tool for reproductive management. In a series of observational studies with up to 158,961 IMP4 records from 4,353 AI events, we characterized predictors of pregnancy per …
Transglutaminase Crosslinked Milk Protein Concentrate And Micellar Casein Concentrate: Impact On The Functionality Of Imitation Mozzarella Cheese Manufactured On A Small Scale Using A Rapid Visco Analyzer, Prafulla Salunke, Lloyd Metzger
Transglutaminase Crosslinked Milk Protein Concentrate And Micellar Casein Concentrate: Impact On The Functionality Of Imitation Mozzarella Cheese Manufactured On A Small Scale Using A Rapid Visco Analyzer, Prafulla Salunke, Lloyd Metzger
Dairy Science and Food Science Faculty Publications: 2022 to Present
In dairy-based imitation mozzarella cheese (IMC) formulations, intact casein is critical and imparts IMC with a firm and elastic, stringy, melted texture. Rennet casein (RCN) is the desired ingredient to provide intact casein in IMC and is preferred over milk protein concentrate (MPC) and micellar casein concentrate (MCC). Transglutaminase (TGase), a crosslinking enzyme, alters the physical properties of MPC or MCC and may change IMC functionality. The objective of this study was to determine the effect of TGase-crosslinked MPC and MCC powders on the functionality of IMCs. The TGase treatment included TGase at 0.3 (L) and 3.0 (H) units/g of …
Preferential Binding Of Polyphenols In Blackcurrant Extracts With Milk Proteins And The Effects On The Bioaccessibility And Antioxidant Activity Of Polyphenols, Ting Mao, Fnu Akshit, Iresha Matiwalage, Subha Sasidharan, Caren Meyn Alvarez, Philip Wescombe, Maneesha S. Mohan
Preferential Binding Of Polyphenols In Blackcurrant Extracts With Milk Proteins And The Effects On The Bioaccessibility And Antioxidant Activity Of Polyphenols, Ting Mao, Fnu Akshit, Iresha Matiwalage, Subha Sasidharan, Caren Meyn Alvarez, Philip Wescombe, Maneesha S. Mohan
Dairy Science and Food Science Faculty Publications: 2022 to Present
Milk proteins are well-known delivery agents; however, there is no clear understanding of the competitive interactions of milk proteins with polyphenols in mixed complex systems. Here, we investigate the preferential competitive interactions of different polyphenols present in blackcurrant extract with milk proteins by quantifying the protein-bound polyphenols and comparing the factors affecting these interactions. In addition, bioaccessibility and antioxidant activity were studied after in vitro gastric digestion. Our results indicated that polyphenols from blackcurrant extracts were preferentially bound to caseins more than whey proteins, with noncovalent interactions causing secondary structural changes in the protein. The hydrophobicity and the charge of …
Changes In Milk Protein Functionality At Low Temperatures And Rennet Concentrations, Mahmoud E. A. Hamouda, Prafulla Salunke
Changes In Milk Protein Functionality At Low Temperatures And Rennet Concentrations, Mahmoud E. A. Hamouda, Prafulla Salunke
Dairy Science and Food Science Faculty Publications: 2022 to Present
This study aimed to evaluate the influence of low-concentration rennet on the chemical, rheological characteristics, and protein fractions of skim milk (SM) at 4 ± 1 °C. Skimmed milk (SM) was divided into four lots of 500 mL, and diluted rennet (1:10,000) was added at different levels at 4 ± 1 °C. The treatments included control (no rennet), T1 (0.001 mL/rennet), T2 (0.01 mL rennet), and T3 (0.1 mL rennet) treatments, which were incubated for 24 h. The sampling was performed at 0, 1, 2, 6, 12, and 24 h, and the SM after incubation time was heated to 73 …
Effects Of Dietary Rumen-Protected Choline Supplementation To Periparturient Dairy Cattle On Inflammation And Metabolism In Mammary And Liver Tissue During An Intramammary Lipopolysaccharide Challenge, Turner H. Swartz, Barry Bradford, Laman Mamedova, Kari Estes
Effects Of Dietary Rumen-Protected Choline Supplementation To Periparturient Dairy Cattle On Inflammation And Metabolism In Mammary And Liver Tissue During An Intramammary Lipopolysaccharide Challenge, Turner H. Swartz, Barry Bradford, Laman Mamedova, Kari Estes
Dairy Science and Food Science Faculty Publications: 2022 to Present
The objective of this experiment was to examine the effects of supplementation and dose of rumenprotected choline (RPC) on markers of inflammation and metabolism in liver and mammary tissue during an intramammary lipopolysaccharide (LPS) challenge. Parous Holstein cows were blocked by calving month and randomly assigned within block to receive 45 g/d of RPC (20.4 g/d of choline ions; CHOL45), 30 g/d of RPC (13.6 g/d of choline ions; CHOL30), or no RPC (CON) as a top-dress starting 24 d before expected calving until 21 d postpartum. Cows were alternately assigned within treatment group to either receive an intramammary LPS …
The Effect Of Soluble Soybean Polysaccharide Addition To Permeate Concentrate On Lactose Crystallisation, Growth And Recovery During Lactose Manufacturing, Venkateswarlu Sunkesula, Prafulla Salunke, Lloyd E. Metzger
The Effect Of Soluble Soybean Polysaccharide Addition To Permeate Concentrate On Lactose Crystallisation, Growth And Recovery During Lactose Manufacturing, Venkateswarlu Sunkesula, Prafulla Salunke, Lloyd E. Metzger
Dairy Science and Food Science Faculty Publications: 2022 to Present
Lactose is manufactured from permeates of cheese whey, milk protein concentrate and a mix of both using a crystallisation process. Several factors, such as solute supersaturation, cooling rate and impurities, influence lactose crystallisation. This study focussed on the effect of soluble soybean polysaccharides (SSPS) on crystallisation during lactose manufacture from concentrated permeate solutions by evaluating lactose crystal growth and recovery with and without SSPS addition. Adding 0.1% (w/w) SSPS resulted in a significant difference (P > 0.05) in lactose crystal size and yield. The results suggest that using SSPS during lactose manufacturing from permeate concentrates could influence lactose crystallisation and recovery
Effects Of Dietary Rumen-Protected Choline Supplementation To Periparturient Dairy Cattle On Inflammation, Metabolism, And Performance During An Intramammary Lipopolysaccharide Challenge, Turner H. Swartz, Barry Bradford, Laman Mamedova, Kari Estes
Effects Of Dietary Rumen-Protected Choline Supplementation To Periparturient Dairy Cattle On Inflammation, Metabolism, And Performance During An Intramammary Lipopolysaccharide Challenge, Turner H. Swartz, Barry Bradford, Laman Mamedova, Kari Estes
Dairy Science and Food Science Faculty Publications: 2022 to Present
Recent studies have suggested that dietary rumen-protected choline (RPC) supplementation can modulate immune function, attenuate inflammation, and improve performance in periparturient dairy cattle; however, this has yet to be evaluated during a mastitis challenge. Therefore, the objective of this study was to examine the effects of supplementation and dose of RPC on metabolism, inflammation, and performance during an intramammary lipopolysaccharide (LPS) challenge. Parous Holstein cows (parity, mean ± SD, 1.9 ± 1.1 at enrollment) were blocked by calving month and randomly assigned within block to receive either 45 g/d of RPC (20.4 g/d of choline ions; CHOL45, n = 18), …
Incorporation Of Probiotics And Other Functional Ingredients In Dairy Fat-Rich Products: Benefits, Challenges, And Opportunities, Kritika Gaba, Sanjeev Anand
Incorporation Of Probiotics And Other Functional Ingredients In Dairy Fat-Rich Products: Benefits, Challenges, And Opportunities, Kritika Gaba, Sanjeev Anand
Dairy Science and Food Science Faculty Publications: 2022 to Present
This review focuses on the potential opportunities to incorporate functional ingredients like probiotics in the dairy fat-rich matrix to develop functional foods. Many dietary guidelines and the relevant literature have in general indicated many benefits of consuming milk-fat-rich products for the human body. Milk fat contains essential nutrients, including fat-soluble vitamins; short-, medium-, and long-chain with odd and branched chain fatty acids; essential amino acids; and calcium, which are all known for their bioactive properties. In addition, the incorporation of probiotics, which are known for their bioactive properties, could further enhance the products’ attributes. However, direct probiotic addition is known …
Impact Of Transglutaminase Treated Micellar Casein Concentrate And Milk Protein Concentrate On The Functionality Of Processed Cheese Product Slice Formulations, Prafulla Salunke, Lloyd E. Metzger
Impact Of Transglutaminase Treated Micellar Casein Concentrate And Milk Protein Concentrate On The Functionality Of Processed Cheese Product Slice Formulations, Prafulla Salunke, Lloyd E. Metzger
Dairy Science and Food Science Faculty Publications: 2022 to Present
Milk protein concentrate (MPC) is used as an ingredient in processed cheese product (PCP) formulations. However, its use can result in texture defects such as a soft body and restricted melting characteristics. The use of micellar casein concentrate (MCC), which has a higher level of casein and less serum protein, improves the texture of PCP. Further improvement in PCP products may be possible using transglutaminase (TGase), an enzyme that can crosslink proteins. This study aimed to determine the effect of TGase treatment of MPC and MCC retentates on the functionality of MPC and MCC when used in a PCP slice …
Microstructural Characterization Of High-Protein Dairy Powders, Prafulla Salunke, Athira Syamala, Lloyd Metzger
Microstructural Characterization Of High-Protein Dairy Powders, Prafulla Salunke, Athira Syamala, Lloyd Metzger
Dairy Science and Food Science Faculty Publications: 2022 to Present
Dairy proteins are potential and multipurpose ingredients used to prepare various food products. It exhibits many beneficial functionalities and bioactivities in the processing of food products. All the functionalities of different dairy proteins depend on their peculiar structural characteristics. So, the present study aimed to characterize high-protein powders with different matrices (milk protein concentrate (MPC), rennet casein (RCN), micellar casein concentrate (MCC), whey protein isolate (WPI), and native whey concentrate (NWC)) for their composition, handling, and reconstitution properties, and microstructure and compared them with whole milk powder (WMP) and skim milk powder (SMP). The bulk density of the high-protein powders …
Perspectives Of Dairy Employees At The Beginning Of The Covid-19 Pandemic: A Survey Of Health Risks And Educational Needs, Fernanda Ferreira, Maristela Rovai, Mireille Chahine, Mario E. De Haro-Martí, John Wenz, Joseph Dalton, Noelia Silva-Del-Rio
Perspectives Of Dairy Employees At The Beginning Of The Covid-19 Pandemic: A Survey Of Health Risks And Educational Needs, Fernanda Ferreira, Maristela Rovai, Mireille Chahine, Mario E. De Haro-Martí, John Wenz, Joseph Dalton, Noelia Silva-Del-Rio
Dairy Science and Food Science Faculty Publications: 2022 to Present
Our objective was to understand dairy employees' perceptions and educational needs at the onset of the COVID-19 pandemic. A bilingual (English and Spanish), anonymous survey targeted at dairy employees was circulated nationwide via university and allied industry media outlets. Responses (n = 63) from 11 states were received (May–Sep. 2020). Respondents worked in herds ranging from 50 to 40,000 animals in size. Dairy managers (33%) responded mostly to the English survey (52%), whereas entry-level workers (67%) chose the Spanish format (76%). Survey results highlighted different perspectives, educational needs, and preferred sources of information between English- and Spanish-speaking dairy workers. Overall, …
Associations Between Preweaning Calf Feeding Behaviors With Age At First Calving And Lactational Performance Using An Automatic Calf Feeder, Turner H. Swartz, Christina Petersson-Wolfe
Associations Between Preweaning Calf Feeding Behaviors With Age At First Calving And Lactational Performance Using An Automatic Calf Feeder, Turner H. Swartz, Christina Petersson-Wolfe
Dairy Science and Food Science Faculty Publications: 2022 to Present
The objective of this retrospective observational study was to determine whether preweaning calf behaviors and milk replacer (MR) intake from an automatic calf feeder were associated with age at first calving and first-lactation performance. Calves were housed in groups with access to an automatic calf feeder for 7 wk with a maximum milk allowance of 1,800 g/d of MR (12 L/d). Outcomes of interest included age at first calving (n = 137), first-lactation mature-equivalent 305-d (ME305) milk yield (n = 132), and first-lactation ME305 energy-corrected milk (ECM) yield (n = 132). Linear models included the fixed effects of the daily …
The Effects Of Dexamethasone Administration On Physiological, Behavioral, And Production Parameters In Dairy Cows After A Difficult Calving, Turner H. Swartz, Dana Bryant, Hollie Schramm, Allison Duncan, Robin White, Cynthia Wood, Christina Petersson-Wolfe
The Effects Of Dexamethasone Administration On Physiological, Behavioral, And Production Parameters In Dairy Cows After A Difficult Calving, Turner H. Swartz, Dana Bryant, Hollie Schramm, Allison Duncan, Robin White, Cynthia Wood, Christina Petersson-Wolfe
Dairy Science and Food Science Faculty Publications: 2022 to Present
Dairy cows are predisposed to diseases during the postpartum period. Dystocia has been associated with increased risk for disease, which is likely the result of increased tissue trauma and stress during the prolonged parturition. To attenuate the inflammatory response seen in dystocic animals and improve well-being, we assessed the effects of a glucocorticoid, dexamethasone administered within 12 h after calving. Dystocia was defined as a difficult birth resulting in a prolonged calving (≥70 min after the amniotic sac appears) and was monitored through 3 video cameras in the close-up dry-cow pen. Cows meeting the dystocia definition were randomly …
Evaluation Of Reticuloruminal Temperature For The Prediction Of Clinical Mastitis In Dairy Cows Challenged With Streptococcus Uberis, Zelmer Rodriguez, Quinn K. Kolar, Kirby C. Krogstad, Turner H. Swartz, Llkyu Yoon, Barry J. Bradford, Pamela L. Ruegg
Evaluation Of Reticuloruminal Temperature For The Prediction Of Clinical Mastitis In Dairy Cows Challenged With Streptococcus Uberis, Zelmer Rodriguez, Quinn K. Kolar, Kirby C. Krogstad, Turner H. Swartz, Llkyu Yoon, Barry J. Bradford, Pamela L. Ruegg
Dairy Science and Food Science Faculty Publications: 2022 to Present
Automated monitoring devices have become increasingly utilized in the dairy industry, especially for monitoring or predicting disease status. While multiple automated monitoring devices have been developed for the prediction of clinical mastitis (CM), limitations in performance or applicability remain. The aims of this study were to (1) detect variations in reticuloruminal temperature (RRT) relative to an experimental intramammary challenge with Streptococcus uberis and (2) evaluate alerts generated automatically based on variation in RRT to predict initial signs of CM in the challenged cows based on severity of clinical signs and the concentration of bacteria (cfu/mL) in the infected quarter separately. …
Efficacy Of A Typical Clean-In-Place Protocol Against In Vitro Membrane Biofilms, Diwakar Singh, Sanjeev Anand
Efficacy Of A Typical Clean-In-Place Protocol Against In Vitro Membrane Biofilms, Diwakar Singh, Sanjeev Anand
Dairy Science and Food Science Faculty Publications: 2022 to Present
This study evaluates the effectiveness of a typical clean-in-place (CIP) protocol against in vitro biofilms on whey reverse osmosis (RO) membranes developed under static condition. Bacterial isolates obtained from RO membrane biofilms were used to develop single and multispecies biofilms under laboratory conditions. A typical commercial CIP protocol was tested against the 24-h-old biofilms, and included 6 sequential treatment steps based on alkali, surfactant, acid, enzyme, a second surfactant, and a sanitizer treatment step. Experiments were conducted in 4 replicates and the data were statistically analyzed. The results revealed a variation in the resistance of mixed-species biofilms against the individual …
Use Of Micellar Casein Concentrate And Milk Protein Concentrate Treated With Transglutaminase In Imitation Cheese Products—Unmelted Texture, Prafulla Salunke, Chenchaiah Marella, J. K. Amamcharla, K. Muthukumarappan, Lloyd Metzger
Use Of Micellar Casein Concentrate And Milk Protein Concentrate Treated With Transglutaminase In Imitation Cheese Products—Unmelted Texture, Prafulla Salunke, Chenchaiah Marella, J. K. Amamcharla, K. Muthukumarappan, Lloyd Metzger
Dairy Science and Food Science Faculty Publications: 2022 to Present
The amount of intact casein provided by dairy ingredients is a critical parameter in dairy-based imitation mozzarella cheese (IMC) formulation because it has a significant effect on unmelted textural parameters such as hardness. From a functionality perspective, rennet casein (RCN) is the preferred ingredient. Milk protein concentrate (MPC) and micellar casein concentrate (MCC) cannot provide the required functionality due to the higher steric stability of casein micelle. However, the use of transglutaminase (TGase) has the potential to modify the surface properties of MPC and MCC and may improve their functionality in IMC. The objective of this study was to determine …
High Protein Powders Fortification Of Nonfat Yoghurt: Impact Of Protein Source, Protein To Total Solids Ratio, Storage, And Seasonality On The Functionality Of Nonfat Yoghurt Made Using Glucono-Δ-Lactone (Gdl), Kartik Shah, Prafulla Salunke, Lloyd Metzger
High Protein Powders Fortification Of Nonfat Yoghurt: Impact Of Protein Source, Protein To Total Solids Ratio, Storage, And Seasonality On The Functionality Of Nonfat Yoghurt Made Using Glucono-Δ-Lactone (Gdl), Kartik Shah, Prafulla Salunke, Lloyd Metzger
Dairy Science and Food Science Faculty Publications: 2022 to Present
Nonfat yoghurt is a popular fermented product across the world. High protein powders such as Skim Milk Powder (SMP), Nonfat Dry Milk (NDM), and Milk Protein Concentrate (MPC) can be utilized in yoghurt formulations. The final Total Solids (TS), source and amount of protein in the formulation, and the physicochemical changes during storage may impact the functionality of yoghurt-type products. The objective of the study was to evaluate the effects of storage of SMP, NDM, MPC40, and MPC70 on the functionality of nonfat yoghurts at three different protein/ TS levels. Additionally, the impact of SMP and NDM manufactured in different …
Effect Of Storage Of Skim Milk Powder, Nonfat Dry Milk And Milk Protein Concentrate On Functional Properties, Kartik Shah, Prafulla Salunke, Lloyd Metzger
Effect Of Storage Of Skim Milk Powder, Nonfat Dry Milk And Milk Protein Concentrate On Functional Properties, Kartik Shah, Prafulla Salunke, Lloyd Metzger
Dairy Science and Food Science Faculty Publications: 2022 to Present
The physicochemical changes during the storage of high protein powders, such as skim milk powder (SMP), nonfat dry milk (NDM), and milk protein concentrates (MPC), can result in a variation in the functional properties of the powders. The objective of this study was to evaluate the effects of the storage of various milk powders (SMP, NDM, MPC40, and MPC70) on their functional properties. Three different lots of the powders were collected from US manufacturers and were analyzed for functional properties after 3, 9, and 15 months of storage at 25 °C. Additionally, this study also evaluated the effects of seasonal …
Perspective Of Dairy Producers From California, Idaho, South Dakota, And Washington: Health And Business Implications Of The Covid-19 Pandemic During The Second Wave, Ainhoa Valldecabres, J. Wenz, Fernanda Ferreira, Mireille Chahine, Joseph Dalton, Mario E. De Haro-Martí, Maristela Rovai, Noelia Silva-Del-Rio
Perspective Of Dairy Producers From California, Idaho, South Dakota, And Washington: Health And Business Implications Of The Covid-19 Pandemic During The Second Wave, Ainhoa Valldecabres, J. Wenz, Fernanda Ferreira, Mireille Chahine, Joseph Dalton, Mario E. De Haro-Martí, Maristela Rovai, Noelia Silva-Del-Rio
Dairy Science and Food Science Faculty Publications: 2022 to Present
The aim of this study was to document California, Idaho, South Dakota, and Washington dairy producers' perspectives on the health and business implications of COVID-19 during the pandemic second wave. Dairy producers were reached by a 14-question anonymous mail survey during June and July of 2020. We obtained 226 responses (response rate: 9.3% CA, 8.6% ID, 31.4%, and 10.0% WA). Responses were grouped by state (CA: 48.7%, ID: 15.9%, SD: 21.7%, or WA: 13.7%) and dairy size [based on number of cows; small (<100): 14.1%, medium (100–499): 27.7%, medium-large (500–1,999): 33.2%, or large (≥2,000): 25.0%]. Survey responses were summarized, and multiple correspondence analysis was used to map responses and identify data clusters. At the time of the survey, some respondents suspected (3%) or had confirmed (9%) COVID-19 cases on their premises. Respondents were somewhat or very concerned about the health (75%) and business (92%) implications associated with COVID-19. Producers were concerned about the health of their families, employees, and employees' families; having to reduce production; workforce shortage; limited goods availability; and lack of services. Producers perceived that their employees were somewhat or very concerned (74%) with the pandemic. There was COVID-19 safety information or training provided (78%) or intended (4%) in English (22%), Spanish (23%), or both (55%). The focus of training was as follows: how to remain healthy at work (91%) and at home (60%), what to do if a worker gets sick (77%), and sick leave information (54%). Nevertheless, 18% of the respondents answered that training was not going to be provided. The following control measures implemented: providing hand sanitizer or disinfectant wipes (78%), instruction on frequent hand-washing (76%), social distancing (59%), prevention of employee gatherings (54%), providing and requiring the use of face masks (49%), and limiting on-farm visitors (44%). Multiple correspondence analysis showed that concern with health …
Evaluation Of The Effects Of Water Quality On Drinking Preferences Of Heifer Calves, Nirosh Senevirathne, Jill Anderson
Evaluation Of The Effects Of Water Quality On Drinking Preferences Of Heifer Calves, Nirosh Senevirathne, Jill Anderson
Dairy Science and Food Science Faculty Publications: 2022 to Present
Drinking water quality requirements focus mainly on removing hazardous substances from the water supply, making it safe to drink and appropriate for livestock use. However, there is a concern that high mineral content in water may affect the drinking behavior of heifer calves, which may affect health and performance. In South Dakota, the primary water sources for agricultural use are surface or groundwater. Water quality varies between different regions of the state; in the eastern part of the state, the water is classified as “hard water” and calcium is the primary mineral found in the water. Our objective was to …