Dairy Science Commons^™

Increased Coding Potential Of Bovine Herpesvirus 1, Victoria Jefferson

Theses and Dissertations

Bovine respiratory disease (BRD) costs the cattle industry millions of dollars in costs in treatment and loss every year in the United States. A significant pathogen often contributes to BRD is Bovine Herpesvirus 1 (BoHV-1), a double stranded DNA virus with the ability to establish latency in the trigeminal ganglia and neurons. Primary infection with BoHV-1 results in immunosuppression that increases the risk of secondary bacterial infection and pneumonia. Because herpesviruses infect their hosts for life and can be reactivated in times of stress, BoHV-1 can present a recurring risk of BRD. The following research aims to expand the knowledge …

An Evaluation Of Bovine Reproductive Function From The Microrna Expression In The Female To Performance Testing In The Male, Erin Amiss

All Theses

Reproductive function is crucial to the success of a beef or dairy herd. Both the female and male play significant roles in the outcome of bovine reproduction. The bull contributes to reproductive success in many ways, such as genetics. The initial role that the bull has in reproductive success is fertilization, whether natural or by artificial insemination. One method of assessing bull performance are central bull test stations, which are traditionally administered by a university, extension center, or are privately owned. Central bull tests assess the performance of bulls from various management systems, breeds, and ages. Some measurements taken during …

Using Cytoplasmic Biopsies To Determine Bovine Oocyte Quality, Madison Lindsey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Embryos resulting from assisted reproductive technologies, such as in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), develop with lower efficiencies than embryos resulting from their in vivo counterparts. The reasons behind the developmental discrepancies remain largely unknown. Because the egg is the primary determinant of embryo developmental success, it is reasonable to consider inherent egg quality as a possible cause. The hypothesis for this project is that there are distinct mRNA transcript patterns, or molecular “fingerprints,” that distinguish high- versus low-quality eggs developing within the same environment. In this study, a small cytoplasmic biopsy was removed from 40 eggs …

Liquid Chromatography With Tandem Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Symone T. Whalin

Masters Theses & Specialist Projects

Bovine mastitis, caused by a wide array of pathogens, results in a substantial economic loss for the dairy cattle livestock industry. β-defensins are a part of the bovine’s innate immune system and act as the first line of defense against mastitis. Only foundational research has been done on β-defensins’ ability to treat and prevent mastitis. There have been no analytical methods reported in the literature for analyzing β-defensins in bovine milk. This research aims to create an analytical approach to determine β-defensins in bovine milk. It is challenging to determine an analyte in a complex sample matrix, and milk is …

Comparison Of Bovine Mammary Involution And Intramammary Infections Following Intramammary Treatment With Casein Hydrolysate And Other Conventional Treatments At Dry-Off, Justine E. Britten, Kerry A. Rood, David J. Wilson

Animal, Dairy, and Veterinary Science Student Research

Alternatives to routine antibiotic treatment of dairy cattle during the dry period before their next calving are of interest. This was a preliminary study of whether intramammary infusion of casein hydrolysate, administered alone or combined with standard dry treatment, accelerated the rate of mammary involution early in the dry period. Four treatments were studied in a split udder design. One udder half was assigned a treatment, and the contralateral half was administered dry cow treatment + internal teat sealant as a control. Treatments were casein hydrolysate, casein hydrolysate + dry cow treatment, casein hydrolysate + teat sealant and casein hydrolysate …

Effects Of Probiotic Supplementation On Growth And Health Of Pre-Weaned Dairy Calves, Lily Bello

Honors Scholar Theses

Dairy calf immune development leaves calves susceptible to illness during the pre-weaned stage of life. Previous studies have shown that the use of probiotics during this time may result in increased growth rates and decreased incidence of illness, particularly diarrhea and scours. We hypothesized that by providing probiotics during the time from birth until weaning, calves on study would experience similar growth and health benefits. Neonatal calves (N=32) were provided milk replacer three times daily, with half of the calves receiving Probios® supplements mixed in with their first morning feeding. Growth and health measurements were collected weekly. Probiotic administration significantly …

Maturation And Culture Media Effects On In Vitro Bovine Embryo Developmental Competence, Ciara M. Helland

Master's Theses

In vitro produced bovine embryos are critical to the cattle industry. However, these embryos have altered morphology, epigenetics, and metabolism when compared to their in vivo counterparts. The aim of this thesis was to alter maturation and culture media to improve the developmental competence of in vitro bovine embryos. This thesis is comprised of three experiments and one proof of concept study. Each experiment followed the same general layout: oocyte aspiration from Jersey or Holstein ovaries, oocyte maturation for 24 hours, fertilization with bull semen for 24 hours, then embryo culture for 7-8 days in 38.5 °C in 5% O …

Prevalence Of Bovine Viral Diarrhea Virus In Bovine Samples From The Intermountain West Of The U.S.A. - Comparison Between Age, Sex, Breed And Diagnostic Methods, David J. Wilson, T. J. Baldwin, E. J. Kelly, A. Van Wettere, G. Hullinger, J. Bunnell

Animal, Dairy, and Veterinary Science Faculty Publications

Prevalence of Bovine viral diarrhea virus (BVDV) (“detected” test results) among all bovine samples tested at the Utah Veterinary Diagnostic Laboratory from 2008 - 2013 was calculated, and results were compared by age, sex, or breed of the cattle and BVDV diagnostic test methods. Necropsies were tested for BVDV when lesions suggestive of infection were identified. Adults, juveniles and most calves were tested by antigen (Ag) capture ELISA, while fetuses and some calves were tested by real-time reverse transcriptase PCR. Cattle originated from Utah and surrounding states. Chi-square analyses were used to test for significant differences in BVDV prevalence between …

Determination Of The Expression Patterns Of Bovine Non-Classical Major Histocompatibility Complex (Mhc) Class I Proteins, Parveen Parasar

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This project was funded by the United States of Department of Agriculture (USDA), which funds research aimed at improving production and animal health. The aim of this study was to advance knowledge of maternal immune tolerance to the fetus and mechanisms bovine non-classical MHC class I proteins employ to interact with immune cells and render them inert towards the fetus.

A fetus is a tissue graft inside the mother’s uterus yet must be accepted by the mother to maintain a successful pregnancy. Reproductive insufficiency and pregnancy failure are major causes of production loss in cattle, especially in cloned animals. Knowledge …

Regulation And Expression Of Nanog, Oct4, And Sox2 In The Bovine Blastocyst Following Somatic Cell Nuclear Transfer, Justin Scott Hall

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Animal, Dairy, and Veterinary Sciences Department (ADVS) and the Center for Integrated Biosystems (CIB) at Utah State University are studying various molecular mechanisms involved in the animal cloning process. This study involves the extensive network of people, facilities, equipment, and funding already associated with the CIB and ADVS joint project.

Cloning involves many molecular challenges that for the most part have become roadblocks for the normal development of the fetus. The mechanisms necessary to transform an adult cell into a competent stem cell that can then transform and develop into a healthy organism are poorly understood. Some of these …

Analysis Of Single Nucleotide Polymorphism Panels For Bovine Dna Identification, Kimberly A. Blanchard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Single nucleotide polymorphisms (SNPs) have great power in resolving questions of unknown parentage on ranches or dairies where errors could have been made in pedigree record keeping or when such records were simply not kept. Currently a panel of 88 SNPs based on a panel originally created by USDA-MARC is commercially available from the company Fluidigm^®. Our objective was to determine whether or not the number of SNPs could be reduced to form a smaller, more cost-efficient parentage-testing panel. A smaller panel would be beneficial to farmers and researchers alike in the reduction of time spent running and …

The Influence Of Fluorescent Light On The Development Of In Vitro Fertilized Bovine Oocytes, Jared Bunch

Undergraduate Honors Capstone Projects

Advances in in vitro fertilization and in vitro culture techniques have allowed considerable progress in identifying physiological requirements of mammalian embryos. Parrish et al. (11) reported a major breakthrough on in vitro fertilization when his group identified heparin as an important factor for the capacitation of spermatozoa. Capacitation is necessary for fertilization of matured oocytes. During the precoculture era of embryos (prior to 1980), the development of early preirnplantation embryos was vary limited regardless of medium, medium supplement, gas atmosphere, osmolarity or pH used (7). Coculture techniques of fertilized oocytes using somatic cells during in vitro production, particularly of bovine …

Histopathology Of Bovine Mastitis, The, C.F. Helmboldt, E.L. Jungherr, W.N. Plastridge

Storrs Agricultural Experiment Station

No abstract provided.

Feeding And Care Of Calves, R. R. Thalman

Nebraska Agricultural Experiment Station: Historical Circulars

It is not infrequent that considerable difficulty is encountered in raising calves with limited amounts of milk or none at all. Requests for information on calf gruels, mixed grain feeds, and commercial supplements are frequent enough to make a short circular upon this subject seem desirable. Furthermore, as the more diversified agricultural program gets under way these requests are increasmg.

White Scours Of Calves, L. Van Es

Nebraska Agricultural Experiment Station: Historical Circulars

It becomes possible to understand some of the reasons for the occurrence of disorders among the newborn of animals kept under the usual conditions imposed by domestication. Among these, the subject of this circular, White Scours in Calves, occupies a prominent place.