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Articles 73471 - 73500 of 658173
Full-Text Articles in Social and Behavioral Sciences
From A Boy To A Leader, Alejandro Zayas
From A Boy To A Leader, Alejandro Zayas
Antioch University Dissertations & Theses
The following autoethnographic dissertation examines my personal experiences of trauma, abuse, and violence. Drawing on journals, memories, and artifacts from my life, I use self-reflection to illustrate the impacts of trauma on my childhood and adulthood. My traumatic experiences of sexual abuse, childhood violence, and emotional abuse are situated within broader sociocultural contexts of masculinity, Hispanic culture, and social norms. This study illuminates possibilities for healing and transformation for myself and others with shared traumatic backgrounds. It calls for trauma-informed education, masculinity, and resiliency. Evocatively sharing my traumatic life events provides an accessible window into often silenced experiences, bearing witness …
Teaching Without Walls: A Portraiture Study Of Nature-Based Educators In Duluth, Minnesota, Christina Wild
Teaching Without Walls: A Portraiture Study Of Nature-Based Educators In Duluth, Minnesota, Christina Wild
Antioch University Dissertations & Theses
This dissertation explores the lived experience of nature-based educators in Duluth, Minnesota. Portraiture served as the methodological framework for learning about how teachers in Duluth got into teaching and why they remain on the job. Teacher turnover and attrition is a national problem exacerbated by the COVID-19 pandemic. Yet, teachers who perceive better working conditions are less likely to leave the profession. In addition, nature-based education has positive influences on students. Past research in nature-based education has looked at students rather than teachers. This study’s findings offer key lessons in the stories of educators who stay in teaching and experience …
Inequitable Distribution Of Plastic Benefits And Burdens On Economies And Public Health, Rachel Karasik, Nancy Lauer, Anne-Elisabeth Baker, Niki E. Lisi, Jason A. Somarelli, William C. Eward, Kathinka Fürst, Meagan M. Dunphy-Daly
Inequitable Distribution Of Plastic Benefits And Burdens On Economies And Public Health, Rachel Karasik, Nancy Lauer, Anne-Elisabeth Baker, Niki E. Lisi, Jason A. Somarelli, William C. Eward, Kathinka Fürst, Meagan M. Dunphy-Daly
Faculty Scholarship
Plastic heterogeneously affects social systems – notably human health and local and global economies. Here we discuss illustrative examples of the benefits and burdens of each stage of the plastic lifecycle (e.g., macroplastic production, consumption, recycling). We find the benefits to communities and stakeholders are principally economic, whereas burdens fall largely on human health. Furthermore, the economic benefits of plastic are rarely applied to alleviate or mitigate the health burdens it creates, amplifying the disconnect between who benefits and who is burdened. In some instances, social enterprises in low-wealth areas collect and recycle waste, creating a market for upcycled goods. …
Toxic Masculinity And Gender-Based Gun Violence In America: A Way Forward, Hayley N. Lawrence
Toxic Masculinity And Gender-Based Gun Violence In America: A Way Forward, Hayley N. Lawrence
Faculty Scholarship
No abstract provided.
Gvsu Undergraduate And Graduate Catalog, 2023-2024, Grand Valley State University
Gvsu Undergraduate And Graduate Catalog, 2023-2024, Grand Valley State University
Course Catalogs, 1963 - Current
Grand Valley State University 2023-2024 undergraduate and graduate course catalog. Course catalogs are published annually to provide students with information and guidance for enrollment.
2023 Adult Foster Home: Resident And Community Characteristics Report On Adult Foster Homes, Ozcan Tunalilar, Sarah Dys, Paula Carder, Diana Jacoby, Sheryl Elliott, Minju Kim, Nathan Finch Parsons, Wafi Albalawi
2023 Adult Foster Home: Resident And Community Characteristics Report On Adult Foster Homes, Ozcan Tunalilar, Sarah Dys, Paula Carder, Diana Jacoby, Sheryl Elliott, Minju Kim, Nathan Finch Parsons, Wafi Albalawi
Institute on Aging Publications
This report is the ninth installment of the Oregon Community-Based Care Study: Resident and Community Characteristics Report on Adult Foster Homes. Each year, the Institute on Aging at Portland State University (IOA/PSU) collects and reports information that can inform and advise policymakers, state and county agency staff, advocates for older adults and people with physical disabilities, and AFH owners and providers about the AFH landscape in Oregon. The results covered in this report include home and owner characteristics, staff positions, wages, and hiring challenges, consumer payer sources, additional fees and services, resident demographics, health conditions, personal care, health services use, …
Obfuscating Systemic Racism: A Critical Policy Discourse Analysis On The Operation Of Neoliberal Ideas In Media Representation Of A School District State Takeover, Trish A. Lopez, Holly Sheppard Riesco, Christian Z. Goering
Obfuscating Systemic Racism: A Critical Policy Discourse Analysis On The Operation Of Neoliberal Ideas In Media Representation Of A School District State Takeover, Trish A. Lopez, Holly Sheppard Riesco, Christian Z. Goering
Curriculum and Instruction Faculty Publications and Presentations
Education reform in the United States has unwisely focused attention on standards and accountability to the state as determined by standardized testing (Berliner & Biddle, 1995; Mehta, 2013). Stemming from the emphasis on standards-based accountability are the ideas of rapid school turnaround and the state’s role in this process (Peck & Reitzug, 2014; VanGronigen & Meyers, 2019). The current study employed critical policy discourse analysis to examine the media’s portrayal of the 2019 determination to continue or terminate state control of the Little Rock School District. The analysis highlights two argumentative frames—one that emphasized neoliberal values in support of continued …
Qualitative Oral-Presentation Feedback: Comparisons From Business Professionals, Instructors, And Student Peers, Matt Baker, William H. Baker
Qualitative Oral-Presentation Feedback: Comparisons From Business Professionals, Instructors, And Student Peers, Matt Baker, William H. Baker
Faculty Publications
Previous studies compare quantitative feedback ratings of student peers and instructors, but new presentation-feedback technologies enable qualitative-feedback comparison. This study extends previous research by comparing qualitative feedback and business professionals’ feedback. Compared to the professionals, the instructors provided similar feedback types and sentiment; students, however, de-emphasized message delivery and made fewer suggestions for improvement. The results suggest that students may need additional practice in critiquing message delivery and in suggesting needed improvements in their peers’ oral presentations. The study also provides a methodology using the new technologies for instructors to calibrate their own and their students’ feedback with professionals’ feedback.
Multiple-Choice Reading Behaviors Of Esl Students: An Eye-Tracking Study, Juan Escalante, Grant Eckstein, Troy L. Cox, Steven Luke
Multiple-Choice Reading Behaviors Of Esl Students: An Eye-Tracking Study, Juan Escalante, Grant Eckstein, Troy L. Cox, Steven Luke
Faculty Publications
Only recently has eye-tracking been used to investigate test-taker reading behavior, and results have been primarily used to confirm a range of cognitive tasks elicited by test items. This study explores test taker reading behavior for its own sake by describing how ESL readers of different proficiency levels behaviorally view multiple-choice passages and test items at different difficulty levels. Data were gathered from 51 students at three proficiency levels attending a university-sponsored intensive English program (IEP). Participants read eight validated reading comprehension items at varying difficulty levels while their eye movements were recorded on the passage, multiple-choice stem, correct answer, …
The Price Of Fairness, Christopher Buccafusco, Daniel Hemel, Eric Talley
The Price Of Fairness, Christopher Buccafusco, Daniel Hemel, Eric Talley
Faculty Scholarship
The COVID-19 pandemic led to acute supply shortages across the country as well as concerns over price increases amid surging demand. In the process, it reawakened a debate about whether and how to regulate “price gouging”—a controversy that continues as inflation has accelerated even as the pandemic abates. Animating this debate is a longstanding conflict between laissez-faire economics, which champions price fluctuations as a means to allocate scarce goods, and perceived norms of consumer fairness, which are thought to cut strongly against sharp price hikes amid shortages.
This Article provides a new, empirically grounded perspective on the price gouging debate …
Twenty-First Century Split: Partisan, Racial, And Gender Differences In Circuit Judges Following Earlier Opinions, Stuart Minor Benjamin, Kevin M. Quinn, Byungkoo Kim
Twenty-First Century Split: Partisan, Racial, And Gender Differences In Circuit Judges Following Earlier Opinions, Stuart Minor Benjamin, Kevin M. Quinn, Byungkoo Kim
Faculty Scholarship
Judges shape the law with their votes and the reasoning in their opinions. An important element of the latter is which opinions they follow, and thus elevate, and which they cast doubt on, and thus diminish. Using a unique and comprehensive dataset containing the substantive Shepard’s treatments of all circuit court published and unpublished majority opinions issued between 1974 and 2017, we examine the relationship between judges’ substantive treatments of earlier appellate cases and their party, race, and gender. Are judges more likely to follow opinions written by colleagues of the same party, race, or gender? What we find is …
Judging Firearms Evidence And The Rule 702 Amendments, Brandon Garrett, Nicholas Scurich, Eric Tucker, Hannah Bloom
Judging Firearms Evidence And The Rule 702 Amendments, Brandon Garrett, Nicholas Scurich, Eric Tucker, Hannah Bloom
Faculty Scholarship
No abstract provided.
The Meta Oversight Board's Human Rights Future, Laurence R. Helfer, Molly K. Land
The Meta Oversight Board's Human Rights Future, Laurence R. Helfer, Molly K. Land
Faculty Scholarship
Responses to the new Oversight Board created by Facebook (now Meta) have run the gamut from enthusiastic to overtly suspicious. Many observers are highly skeptical of the Board’s ability to hold Meta accountable or to protect the company’s users. Much of this skepticism is based on a misplaced analogy between the Board and domestic courts. We argue, in contrast, that the core challenges and opportunities that the Oversight Board faces are similar to those confronted by international human rights tribunals. Analyzing the Oversight Board as a de facto human rights tribunal sheds light on the strengths and weaknesses of its …
The Challenge Of Measuring Traditional And Digital Audiences In A Global Market, Jorge Gallardo-Camacho, César García, Belén Puebla-Martínez
The Challenge Of Measuring Traditional And Digital Audiences In A Global Market, Jorge Gallardo-Camacho, César García, Belén Puebla-Martínez
All Faculty Scholarship for the College of Arts and Humanities
This monograph titled Audiences and new forms of broadcast: linear, on-demand, streaming and/or social has five articles that represent the complexity of the phenomenon of audiences across fields and from different perspectives: Traditional television consumption, viewing of Netflix, the social audience of video platforms, the new tastes of viewers for vertical formats driven by mobile phones, and the relationship of influencers with their audiences. This number raises the problem of audience measurement, quantification, and comparison in the new digital age. The measurement of audiovisual audiences faces the problem of the lack of a measurement system accepted in all regions and …
Toxic Timescapes: Examining Toxicity Across Time And Space, Simone M. Müller, May-Brith Ohman Nielsen
Toxic Timescapes: Examining Toxicity Across Time And Space, Simone M. Müller, May-Brith Ohman Nielsen
Ohio University Press Open Access Books
An interdisciplinary environmental humanities volume that explores human-environment relationships on our permanently polluted planet.
While toxicity and pollution are ever present in modern daily life, politicians, juridical systems, media outlets, scholars, and the public alike show great difficulty in detecting, defining, monitoring, or generally coming to terms with them. This volume’s contributors argue that the source of this difficulty lies in the struggle to make sense of the intersecting temporal and spatial scales working on the human and more-than-human body, while continuing to acknowledge race, class, and gender in terms of global environmental justice and social inequality.
The term toxic …
Security Investigations: A Professional's Guide, Alan Saquella, Larry Nicholson
Security Investigations: A Professional's Guide, Alan Saquella, Larry Nicholson
Publications
From the forward:
"Security Investigations A Professional’s Guide has been expertly crafted to equip both novices and experienced investigators with the essential knowledge and skill to excel in the field of investigations. Whether aiming for a career in law enforcement, private investigations, corporate security, or you are simply interested in unraveling mysteries, this book will serve as your ultimate companion on the journey to becoming a proficient investigator."
Chapters 1 and 2 are presented here.
Determining Child Sexual Abuse Posts Based On Artificial Intelligence, Susan Mckeever, Christina Thorpe, Vuong Ngo
Determining Child Sexual Abuse Posts Based On Artificial Intelligence, Susan Mckeever, Christina Thorpe, Vuong Ngo
Conference papers
The volume of child sexual abuse materials (CSAM) created and shared daily both surface web platforms such as Twitter and dark web forums is very high. Based on volume, it is not viable for human experts to intercept or identify CSAM manually. However, automatically detecting and analysing child sexual abusive language in online text is challenging and time-intensive, mostly due to the variety of data formats and privacy constraints of hosting platforms. We propose a CSAM detection intelligence algorithm based on natural language processing and machine learning techniques. Our CSAM detection model is not only used to remove CSAM on …
Queer In Ai: A Case Study In Community-Led Participatory Ai, Anaelia Ovalle, Arjun Subramonian, Ashwiin Singh, Claas Voelcker, Danica Sutherland, Davide Locatelli, Eva Breznik, Felip Klubicka, Hang Yuan, Hetvi J, Huan Zhang, Jaidev Shriram, Kruno Lehman, Luca Soldaini, Maarten Sap, Marc Peter Deisenroth, Maria Leonor Pacheco, Maria Ryskina, Martin Mundt, Melind Agarwal, Nyx Mclean, Pan Xu, A. Pranav, Raj Korpan, Ruchira Ray, Sarah Mathew, Sarthak Arora, S.T. John, Tanvi Anand, Vishakha Agrawal, William Agnew, Yanan Long, Zijie J. Wang, Zeerak Talat, Avijit Ghosh, Nathaniel Dennler, Michael Noseworthy, Sharvani Jha, Emi Baylor, Aditya Joshi, Natalia Y. Bilenko, Andrew Mcnamara, Raphael Gontijo-Lopes, Alex Markham, Evyn Dong, Jackie Kay, Manu Saraswat, Nikhil Vytla, Luke Stark
Queer In Ai: A Case Study In Community-Led Participatory Ai, Anaelia Ovalle, Arjun Subramonian, Ashwiin Singh, Claas Voelcker, Danica Sutherland, Davide Locatelli, Eva Breznik, Felip Klubicka, Hang Yuan, Hetvi J, Huan Zhang, Jaidev Shriram, Kruno Lehman, Luca Soldaini, Maarten Sap, Marc Peter Deisenroth, Maria Leonor Pacheco, Maria Ryskina, Martin Mundt, Melind Agarwal, Nyx Mclean, Pan Xu, A. Pranav, Raj Korpan, Ruchira Ray, Sarah Mathew, Sarthak Arora, S.T. John, Tanvi Anand, Vishakha Agrawal, William Agnew, Yanan Long, Zijie J. Wang, Zeerak Talat, Avijit Ghosh, Nathaniel Dennler, Michael Noseworthy, Sharvani Jha, Emi Baylor, Aditya Joshi, Natalia Y. Bilenko, Andrew Mcnamara, Raphael Gontijo-Lopes, Alex Markham, Evyn Dong, Jackie Kay, Manu Saraswat, Nikhil Vytla, Luke Stark
Conference papers
Queerness and queer people face an uncertain future in the face of ever more widely deployed and invasive artificial intelligence (AI). These technologies have caused numerous harms to queer people, including privacy violations, censoring and downranking queer content, exposing queer people and spaces to harassment by making them hypervisible, deadnaming and outing queer people. More broadly, they have violated core tenets of queerness by classifying and controlling queer identities. In response to this, the queer community in AI has organized Queer in AI, a global, decentralized, volunteer-run grassroots organization that employs intersectional and community-led participatory design to build an inclusive …
Cognitive Processing Disruptions Affecting Flight Deck Performance: Implications For Cognitive Resilience, Sam J. Holley, Mark D. Miller
Cognitive Processing Disruptions Affecting Flight Deck Performance: Implications For Cognitive Resilience, Sam J. Holley, Mark D. Miller
Publications
The flight deck of a commercial aircraft has become progressively digitized and operates in multiple modes with displays and indicators that require increasing levels of comprehension. Examining several aspects of cognitive processing is important to understand how threats to safety might occur and what actions might be taken to reduce severity or to eliminate the threat altogether. This paper presents the elements of cognition to consider, relevant characteristics of working memory and cognitive processing speed, types of disruptions and how they are addressed, results from overload or confusion, and the need for effective cognitive resilience to recover and repair the …
Economic Rights And Human Dignity, Lucile Sabas, Syoum Negassi
Economic Rights And Human Dignity, Lucile Sabas, Syoum Negassi
Faculty Publications
No abstract provided.
Flying Blind: Exploring The Visual Cues Used By Helicopter Pilots In Degraded Visual Environments, Brandon Dreslin, Molly C. Mersinger, Shivani Patel, Alex Chaparro
Flying Blind: Exploring The Visual Cues Used By Helicopter Pilots In Degraded Visual Environments, Brandon Dreslin, Molly C. Mersinger, Shivani Patel, Alex Chaparro
Publications
Helicopter pilots rely on visual cues from the environment and instrument displays during critical phases of flight – particularly final approach and landing – to safely land. However, the specific visual cues pilots rely on and how they integrate those cues to make anticipatory inceptor inputs or corrections are not well understood. Importantly, those cues may be degraded under nighttime and brownout/whiteout conditions where the downwash of a helicopter’s rotors cause loose dirt/snow to be projected into the air, resulting in the obfuscation of the pilot’s vision outside the aircraft. The lack of visual cues in these conditions means that …
Racially Motivated Police Brutality Is A Community Public Health Issue In The United States, Darrell Norman Burrell, Sharon L. Burton, Grace E. Mcgrath
Racially Motivated Police Brutality Is A Community Public Health Issue In The United States, Darrell Norman Burrell, Sharon L. Burton, Grace E. Mcgrath
Publications
Ongoing global protests against police violence and racism were heightened in 2020 after the deaths of Breana Taylor and George Floyd, whose deaths made headlines worldwide and raised awareness of the Black Lives Matter movement, which targets structural racism and violence against Black people in the USA and abroad. There are many documented links between policing and health-related outcomes, including but not limited to fatal injuries that increase population-specific mortality rates, adverse physiological responses that increase morbidity, psychological stress, arrests, incarcerations, and legal, medical, and funeral bills that cause socioeconomic deprivation, poor school performance, incomplete high-school education and not entering …
Public Procurement For Innovation Through Supplier Firms' Sustainability Lens: A Systematic Review And Research Agenda, Peter Adjei-Bamfo, Hadrian Geri Djajadikerta, Ferry Jie, Kerry Brown, Reza Kiani Mavi
Public Procurement For Innovation Through Supplier Firms' Sustainability Lens: A Systematic Review And Research Agenda, Peter Adjei-Bamfo, Hadrian Geri Djajadikerta, Ferry Jie, Kerry Brown, Reza Kiani Mavi
Research outputs 2022 to 2026
Public sector purchasing processes are gaining increasing prominence as a demand-side innovation tool for addressing national sustainability challenges. Accordingly, there has been growing research attention to this topic. Prior studies suggest three key rationales that underlie the use of public sector procurement to drive innovation: (i) the buyer–user rationale (for creating new needs); (ii) the market/system failure rationale (for improving suppliers' capacity to innovate); and (iii) the public services rationale (for improving public services). However, operational activities at the upstream supply chain affecting the sustainable innovation capacities of supplier firms appear to be under-researched in the public procurement for innovation …
School Psychologists’ Perspectives On The Impact Of Clinical Practice On Their Personal Lives, Nantha Priya Ramu
School Psychologists’ Perspectives On The Impact Of Clinical Practice On Their Personal Lives, Nantha Priya Ramu
Research Datasets
School psychologists in WA completed an online survey which included demographic information and a perception survey which asked them questions relating to their emotional states in the last 6 months, if they felt work impacted their personal relationships and communication, some factors that contributed to wellbeing, self-care practices and organisational practices for school psychologists' wellbeing.
Determinants Of Social Loneliness And Its Association With Negative Coping Styles, Mark Facey
Determinants Of Social Loneliness And Its Association With Negative Coping Styles, Mark Facey
Research Datasets
The original study linked to this dataset argued that underlying antecedents of social loneliness maintain negative behaviour, whereby it becomes chronic and problematic. Four intrinsic antecedents of social loneliness are examined (Secure and Insecure Attachment, Emotional Regulation Problems – Expressive Suppression, Unclear Self-Concept, and Neuroticism) along with negative (Ignoring and Withdrawing) behavioural coping styles. Attachment type, Neuroticism, and to a lesser extent, Self-Concept Clarity, Expressive Suppression and Withdrawal coping styles may play an important role in averting or predicting Social Loneliness. Behaviourally, those who are higher in Social Loneliness, particularly young adult females, may adopt less Withdrawal coping styles once …
Enabling Customization Of Discussion Forums For Blind Users, Mohan Sunkara, Yash Prakash, Hae-Na Lee, Sampath Jayarathna, Vikas Ashok
Enabling Customization Of Discussion Forums For Blind Users, Mohan Sunkara, Yash Prakash, Hae-Na Lee, Sampath Jayarathna, Vikas Ashok
Computer Science Faculty Publications
Online discussion forums have become an integral component of news, entertainment, information, and video-streaming websites, where people all over the world actively engage in discussions on a wide range of topics including politics, sports, music, business, health, and world affairs. Yet, little is known about their usability for blind users, who aurally interact with the forum conversations using screen reader assistive technology. In an interview study, blind users stated that they often had an arduous and frustrating interaction experience while consuming conversation threads, mainly due to the highly redundant content and the absence of customization options to selectively view portions …
Mitigating Anomalous Electricity Consumption In Smart Cities Using An Ai-Based Stacked-Generalization Technique, Arshid Ali, Laiq Khan, Nadeem Javaid, Safdar Hussain Bouk, Abdulaziz Aldegheishem, Nabil Alrahjeh
Mitigating Anomalous Electricity Consumption In Smart Cities Using An Ai-Based Stacked-Generalization Technique, Arshid Ali, Laiq Khan, Nadeem Javaid, Safdar Hussain Bouk, Abdulaziz Aldegheishem, Nabil Alrahjeh
Computer Science Faculty Publications
Energy management and efficient asset utilization play an important role in the economic development of a country. The electricity produced at the power station faces two types of losses from the generation point to the end user. These losses are technical losses (TL) and non-technical losses (NTL). TLs occurs due to the use of inefficient equipment. While NTLs occur due to the anomalous consumption of electricity by the customers, which happens in many ways; energy theft being one of them. Energy theft majorly happens to cut down on the electricity bills. These losses in the smart grid (SG) are the …
Claimdistiller: Scientific Claim Extraction With Supervised Contrastive Learning, Xin Wei, Md Reshad Ul Hoque, Jian Wu, Jiang Li
Claimdistiller: Scientific Claim Extraction With Supervised Contrastive Learning, Xin Wei, Md Reshad Ul Hoque, Jian Wu, Jiang Li
Computer Science Faculty Publications
The growth of scientific papers in the past decades calls for effective claim extraction tools to automatically and accurately locate key claims from unstructured text. Such claims will benefit content-wise aggregated exploration of scientific knowledge beyond the metadata level. One challenge of building such a model is how to effectively use limited labeled training data. In this paper, we compared transfer learning and contrastive learning frameworks in terms of performance, time and training data size. We found contrastive learning has better performance at a lower cost of data across all models. Our contrastive-learning-based model ClaimDistiller has the highest performance, boosting …
Deeppatent2: A Large-Scale Benchmarking Corpus For Technical Drawing Understanding, Kehinde Ajayi, Xin Wei, Martin Gryder, Winston Shields, Jian Wu, Shawn M. Jones, Michal Kucer, Diane Oyen
Deeppatent2: A Large-Scale Benchmarking Corpus For Technical Drawing Understanding, Kehinde Ajayi, Xin Wei, Martin Gryder, Winston Shields, Jian Wu, Shawn M. Jones, Michal Kucer, Diane Oyen
Computer Science Faculty Publications
Recent advances in computer vision (CV) and natural language processing have been driven by exploiting big data on practical applications. However, these research fields are still limited by the sheer volume, versatility, and diversity of the available datasets. CV tasks, such as image captioning, which has primarily been carried out on natural images, still struggle to produce accurate and meaningful captions on sketched images often included in scientific and technical documents. The advancement of other tasks such as 3D reconstruction from 2D images requires larger datasets with multiple viewpoints. We introduce DeepPatent2, a large-scale dataset, providing more than 2.7 million …
Progenitor Cell Isolation From Mouse Epididymal Adipose Tissue And Sequencing Library Construction, Qianglin Liu, Chaoyang Li, Yuxia Li, Leshan Wang, Xujia Zhang, Buhao Deng, Peidong Gao, Mohammad Shiri, Fozi Alkaifi, Junxing Zhao, Jacqueline M. Stephens, Constantine A. Simintiras, Joseph Francis, Jiangwen Sun, Xing Fu
Progenitor Cell Isolation From Mouse Epididymal Adipose Tissue And Sequencing Library Construction, Qianglin Liu, Chaoyang Li, Yuxia Li, Leshan Wang, Xujia Zhang, Buhao Deng, Peidong Gao, Mohammad Shiri, Fozi Alkaifi, Junxing Zhao, Jacqueline M. Stephens, Constantine A. Simintiras, Joseph Francis, Jiangwen Sun, Xing Fu
Computer Science Faculty Publications
Here, we present a protocol to isolate progenitor cells from mouse epididymal visceral adipose tissue and construct bulk RNA and assay for transposase-accessible chromatin with sequencing (ATAC-seq) libraries. We describe steps for adipose tissue collection, cell isolation, and cell staining and sorting. We then detail procedures for both ATAC-seq and RNA sequencing library construction. This protocol can also be applied to other tissues and cell types directly or with minor modifications.
For complete details on the use and execution of this protocol, please refer to Liu et al. (2023).1
*1 Liu, Q., Li, C., Deng, B., Gao, P., …