Dairy Science Commons^™

Histopathologic Evaluation Of Postmortem Autolytic Changes In Bluegill (Lepomis Macrohirus) And Crappie (Pomoxis Anularis) At Varied Time Intervals And Storage Temperatures, Jami George, Arnaud J. Van Wettere, Blayk B. Michaels, Debbi Crain, Gregory A. Lewbart

Animal, Dairy, and Veterinary Science Faculty Publications

Information is lacking on preserving fish carcasses to minimize postmortem autolysis artifacts when a necropsy cannot be performed immediately. The purpose of this study was to qualitatively identify and score histologic postmortem changes in two species of freshwater fish (bluegill - Lepomis macrochirus; crappie - Pomoxis annularis), at varied time intervals and storage temperatures, to assess the histologic quality of collected samples. A pooled sample of 36 mix sex individuals of healthy bluegill and crappie were euthanized, stored either at room temperature, refrigerated at 4 C, or frozen at 20, and then necropsied at 0, 4, 24, and 48 h …

Analysis Of Johne's Disease Elisa Status And Associated Performance Parameters In Irish Dairy Cows, A. E. Kennedy, N. Byrne, A. B. Garcia, Jim O'Mahony, R. G. Sayers

Department of Biological Sciences Publications

Background

Infection with Mycobacterium avium subspecies paratuberculosis (MAP) has been associated with reductions in milk production in dairy cows and sub optimal fertility. The aim of this study was to highlight the production losses associated with testing MAP ELISA positive in Irish dairy cows. Secondary objectives included investigation of risk factors associated with testing MAP ELISA positive. A survey of management practices on study farms was also conducted, with examination of associations between management practices and herd MAP status.

Blood samples were collected from 4188 breeding animals on 22 farms. Samples were ELISA tested using the ID Screen Paratuberculosis Indirect …

Analysis Of Johne’S Disease Elisa Status And Associated Performance Parameters In Irish Dairy Cows, A. E. Kennedy, N. Byrne, A. B. Garcia, Jim O'Mahony, R. G. Sayers

Department of Biological Sciences Publications

Infection with Mycobacterium avium subspecies paratuberculosis (MAP) has been associated with reductions in milk production in dairy cows and sub optimal fertility. The aim of this study was to highlight the production losses associated with testing MAP ELISA positive in Irish dairy cows. Secondary objectives included investigation of risk factors associated with testing MAP ELISA positive. A survey of management practices on study farms was also conducted, with examination of associations between management practices and herd MAP status.

Blood samples were collected from 4188 breeding animals on 22 farms. Samples were ELISA tested using the ID Screen Paratuberculosis Indirect Screening …

Short Communication: Effect Of Storage Temperature On The Solubility Of Milk Protein Concentrate 80 (Mpc80) Treated With Nacl Or Kcl, V. Sikand, P.S. Tong, J. Walker, T. Wang, L.E. Rodriguez-Saona

Dairy Science

A previous study in our laboratory showed that addition of 150 mM NaCl or KCl into diafiltration water improved the solubility of freshly made milk protein concentrate 80 (MPC80). In the present study, the objectives were (1) to evaluate the solubility of NaCl- or KCl-treated MPC80 samples kept at varying temperatures and then stored for extensive periods at room temperature (21°C ± 1°C); and (2) to determine if MPC80 samples stored at different temperatures and protein conformation can be grouped or categorized together. Freshly manufactured MPC80 samples were untreated (control), processed with NaCl, or processed with KCl. One set …

Circulating Amino Acids In Blood Plasma During The Peripartal Period In Dairy Cows With Different Liver Functionality Index, Z. Zhou, J. J. Loor, F. Piccioli-Cappelli, F. Librandi, G. E. Lobley, E. Trevisi

Physiology Collection

The liver functionality index (LFI) measures the changes of albumin, cholesterol, and bilirubin concentrations between 3 and 28 d postpartum. This composite index, based on variables with direct relevance to liver-specific plasma protein synthesis (albumin), hepatic/intestinal lipoprotein synthesis (cholesterol), and clearance of breakdown products of heme catabolism (bilirubin), provides a tool for evaluating manifestations of hepatic disease. Both energy and protein metabolism are likely to be affected by various physiological challenges in this period but have not been tested systematically. The present study was conducted to profile AA in cows with high or low LFI during the peripartal period …

Evaluation Of Calving Indicators Measured By Automated Monitoring Devices To Predict The Onset Of Calving In Holstein Dairy Cows, V. Ouellet, E. Vasseur, W. Heuwieser, O. Burfeind, X. Maldague, É. Charbonneau

Physiology Collection

Dystocias are common in dairy cows and often adversely affect production, reproduction, animal welfare, labor, and economics within the dairy industry. An automated device that accurately predicts the onset of calving could potentially minimize the effect of dystocias by enabling producers to intervene early. Although many well-documented indicators can detect the imminence of calving, research is limited on their effectiveness to predict calving when measured by automated devices. The objective of this experiment was to determine if a decrease in vaginal temperature (VT), rumination (RT), and lying time (LT), or an increase in lying bouts (LB), as measured by …

Culturally Speaking: Ways To Tame Our Sweet Tooth, Phillip Tong

Dairy Science

No abstract provided.

Effect Of Green Tea On Interaction Of Lipid Oxidation Products With Sarcoplasmic And Myofibrillar Protein Homogenates Extracted From Bovine Top Round Muscle, Nahathai Stapornkul, Tatiana Prytkova, Lilian Were

Food Science Faculty Articles and Research

The interaction between lipid oxidation products and bovine sarcoplasmic (SP) and myofibrillar protein (MP) homogenates in the presence of green tea was investigated. To monitor the effect of green tea on lipid oxidation, aldehydes were measured while effect on protein was monitored via changes in myoglobin, thiols, and tryptophan fluorescence over nine days of refrigerated storage. The presence of SP and MP decreased free aldehydes in the buffers. The SP bound more aldehydes than MP. The tea compounds exhibited more favorable binding energies than aldehydes near histidine 64 close to the heme moiety of myoglobin. Addition of tea lowered tryptophan …

The Jackrabbit Dairy Review, Vikram V. Mistry

The Jackrabbit Dairy & Food Review

No abstract provided.

Dry Period Plane Of Energy: Effects On Glucose Tolerance In Transition Dairy Cows, S. Mann, F. A. Leal Yepes, M. Duplessis, J. J. Wakshlag, T. R. Overton, B. P. Cummings, D. V. Nydam

Physiology Collection

Overfeeding energy in the dry period can affect glucose metabolism and the energy balance of transition dairy cows with potential detrimental effects on the ability to successfully adapt to early lactation. The objectives of this study were to investigate the effect of different dry cow feeding strategies on glucose tolerance and on resting concentrations of blood glucose, glucagon, insulin, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) in the peripartum period. Cows entering second or greater lactation were enrolled at dry-off (57 d before expected parturition) into 1 of 3 treatment groups following a randomized block design: cows …

Body Condition Score And Plane Of Nutrition Prepartum Affect Adipose Tissue Transcriptome Regulators Of Metabolism And Inflammation In Grazing Dairy Cows During The Transition Period, M. Vailati-Riboni, M. Kanwal, O. Bulgari, S. Meier, N. V. Priest, C. R. Burke, J. K. Kay, S. Mcdougall, M. D. Mitchell, C. G. Walker, M. Crookenden, A. Heiser, J. R. Roche, J. J. Loor

Physiology Collection

Recent studies demonstrating a higher incidence of metabolic disorders after calving have challenged the management practice of increasing dietary energy density during the last ~3 wk prepartum. Despite our knowledge at the whole-animal level, the tissue-level mechanisms that are altered in response to feeding management prepartum remain unclear. Our hypothesis was that prepartum body condition score (BCS), in combination with feeding management, plays a central role in the peripartum changes associated with energy balance and inflammatory state. Twenty-eight mid-lactation grazing dairy cows of mixed age and breed were randomly allocated to 1 of 4 treatment groups in a 2 × …

Dairy Digest 2016, South Dakota State University Dairy Club

Dairy Digest

No abstract provided.

An Investigation Of The Use Of Natural Agents To Control Mycobacteria, Olga Lesniak

Theses

Economic losses that are caused by Mycobacterium avium paratuberculosis (MAP) infections represent a major obstacle for farmers and also for the dairy industry. In order to control the population of MAP, alternative control agents must be investigated. One of the studies described in this thesis focused on using a bacteriophage therapy based approach to assess its efficacy on simulated industrial environments and food models. Another study described in this thesis investigated the antimicrobial effects of parsley and garlic on a range of mycobacteria species. Both studies used conventional microbiological methods such as plaque assays, AlamarBlue assay (MABA) and growth curves, …

Development And Validation Of Gas Chromatographic Methods For Free Fatty Acid Determination In Dairy Products, David Mannion

Theses

Quantification of free fatty acids (FFA) in dairy products by gas chromatography flame ion'^ation detection is an important requirement for qua'ity, research, nutritional, authenticity and legislative purposes. Common procedures are typically variants of either the fatty acid methyl esterification (FAME) or direct on-column injection methods developed more than two decades ago. This study involved the development of an alternative novel derivatization method using butylation, incorporating automation, reducing solvent usage that is applicable for a wide range of dairy products. The accuracy, precision, linearity, limits of detection and limits of quantification of all three methods were assessed for the quantification of …

Manufacture, Concentration, And Functionality Of Micellar Casein Concentrate, Dustin Grossbier

Electronic Theses and Dissertations

This research has been structured into 3 major parts. The first part evaluates a centrifugal evaporator to concentrate micellar casein concentrate (MCC). The second part evaluates a wiped film evaporator (WFE) to concentrate MCC. The third part evaluates heat stability of the high concentration MCC produced by WFE. In part 1, the primary objective was to achieve >25% total solids (TS) without a loss of water removal rate associated with high solids materials. This is of importance, industrially, as this could reduce processing costs or eliminate drying all together. The major finding was that it did not offer any benefits …

Three Overlooked Issues: Water Quality, Forage Quality, And Forage Blends Impacting The Feed Efficiency Of Dairy Cows, Ishwary Prasad Acharya

Electronic Theses and Dissertations

The overall objectives of the six studies were to increase the feed efficiency in lactating dairy cows. The first study evaluated the efficacy of different water sources and treatment systems on ruminal parameters using an in vitro gas production system. Outcomes of the study suggested that the source and nutrient quality of water can affect rate of ruminal fermentation. Thus, use of real farm water being offered to the cow to conduct in vitro gas production measurements may accurately predict the ruminal fermentation, digestibility and total gas production. The second study evaluated two recently developed leafy-floury corn silage hybrids against …

An Evaluation Of Feeding A Blend Of Essential Oils And Cobalt Lactate To Lactating Dairy Cows, Olivia Jayne Kuester

Electronic Theses and Dissertations

The objective of this study was to determine if the inclusion of a blend of essential oils and cobalt lactate (EOC) in the diet of lactating dairy cows could result in improved nutrient utilization and lactational performance in addition to assessing the application of EOC to control feed mycotoxins and spoilage. The trial was conducted in two experimental periods (Phase 1 (P1) and Phase 2 (P2)) on a commercial dairy in southwest Minnesota equipped with two robotic milking units. Phases 1 and 2 were cross over design trials with two pens. Cows were housed in two freestall pens (57 ± …

Improving Feed Efficiency Through Forage Strategies For Increasing Dairy Profitability And Sustainability, Jon Patrick Pretz

Electronic Theses and Dissertations

Three studies were conducted to determine production parameters and study specific hypothesis in regard to improving feed efficiency through various forage utilization strategies with or without the inclusion of various supplemented products. The first study evaluated the supplementation of a cobalt-lactate product and its effects on fiber digestibility and milk production parameters when fed to cows consuming a 70% forage diet. Treatments included: 1) CONTROL diet containing 12.5 mg/cow/d of cobalt (carbonate carbonate) and 2) TEST diet being the same basal diet but including an additional 50 mg/cow/d of cobalt via a 1% Co-lactate product (Co-Max®). In a feeding trial …

Effects Of Bran Treatment On Rheology And Sensory Quality Of Whole Wheat Flat Bread, Jigyasha Mishra

Electronic Theses and Dissertations

The purpose of this research was to study the differences in the quality of 100% whole wheat flour (WWF) when the bran fraction was subjected to mild solvent treatment before reconstitution with the endosperm. The bran treatments included water washing and washing with ethanol solutions (50% and 100%). WWF prepared with untreated bran served as the control. Effects of these treatments on particle size, chemical composition, color, dough rheology, product formulation, and sensory characteristics of 100% whole wheat flour were studied. Grains of five HRSW cultivars (Advance, Prevail, Select, Brick and Forefront) were milled using a Quadrumat Senior mill. Gluten …

Growth Performance, Nutrient Utilization, And Metabolic Profile Of Dairy Heifers Fed Diets High In Distillers Grains With Different Forage To Concentrate Ratios, Angela Kristia Manthey

Electronic Theses and Dissertations

Two studies were conducted to evaluate the effect of limit-feeding heifers distillers grains with solubles (DDGS) with varying forage to concentrate ratios. The effects on growth, feed efficiency, rumen fermentation, nutrient digestibility, metabolic profile and onset of puberty, as well as post trial performance were investigated. First, a 16-wk feeding trial was conducted using 48 Holstein heifers to evaluate effects of dietary treatment on dry matter intake (DMI), average daily gain (ADG), growth performance, rumen fermentation, and nutrient digestibility. Treatments were 1) 30% DDGS, with the diet fed at 2.65% of body weight (BW) (30DG), 2) 40% DDGS, with the …

An Evaluation Of Rumen Modifiers For Lactational Performance And Nutrient Digestibility By Cows, Prakash Poudel

Electronic Theses and Dissertations

Three studies were conducted to evaluate a commercial blend of essential oils, active dried yeast product and cobalt lactate on production performance, rumen fermentation and nutrient digestibility by cows by using in vivo and in vitro methods. The first study evaluated the lactational performance and nutrient digestibility of lactating Holstein dairy cows fed two commercial supplements: 1) a propriety blend of essential oils and Cobalt lactate (EOC) (Stay Strong, Ralco, Inc., Marshall, MN) and 2) active dried yeast (ADY) (Omnigen AF, Prince Agri Products, Inc., Quincy, IL). The 12-week experiment demonstrated no differences in milk production, milk composition, and feed …

Role Of Exopolymeric Substances In Biofilm Formation On Dairy Separation Membranes, Nuria Garcia-Fernandez

Electronic Theses and Dissertations

In the concentration of cheese whey by membrane filtration processes, fouling is a major limiting factor that leads to flux decline and affects product quality. The growth of biofilms on membranes contributes to biofouling. Biofilms are very difficult to eliminate, and will serve as the seed for additional biofilm development and product contamination. The biofilm matrix is composed mainly of microbial exopolymeric substances (EPS), and therefore EPS is thought to help bacteria to adhere and form biofilms by acting as a cementing agent. The first research project under this study was conducted with the aim of elucidating the role of …

Study Of The Effect Of Feeding Highly Digestible Corn Based Calf Starter On The Growth Performance Of Calves, Shruti Srivastava

Electronic Theses and Dissertations

New corn hybrids have been developed by Masters Choice (MC) that vary in energy density and starch digestibility. These MC hybrids have lower starch densities due to an altered starch structure, which allows for greater ruminal and intestinal starch digestion. The altered starch structure of these new hybrids results in reduced feed manufacturing costs (i.e., grinding). Thirty (30) 1 to 3 d old (40.6 ± 1.72 kg) Holstein heifer calves were randomly assigned to 1 of 2 calf starters to measure growth performance of Holstein heifer calves through 8 weeks of age. Treatments were: 1) Hard starch (HS) corn calf …

Inactivation Of Thermodynamic Sporeformers And Spores In Skim Milk By Continuous Ultrasonication And Hydrodynamic Cavitation In Combination With Thermal Treatments, Dikshi Bawa

Electronic Theses and Dissertations

Studies were conducted to determine the effectiveness of ultrasonication and hydrodynamic cavitation, in a continuous mode, alone and in combination with thermal treatments, on heat resistant aerobic sporeformers and spores in skim milk, and skim milk concentrate. The first study was conducted to investigate the effectiveness of a continuous ultrasonication process to reduce thermoduric Bacillus coagulans cells in skim milk. A 25% reduction was obtained by lab pasteurization alone, whereas ultrasonication alone resulted in an inactivation of 92% after 12 passes (80 sec exposure time per pass). Ultrasonication when combined with pasteurization resulted in further increased inactivation to 99.98%. It …

Impact Of New Processing Technologies On The Physical And Functional Properties Of Skim Milk And Skim Milk Concentrate, Harsh Dahiya

Electronic Theses and Dissertations

United States is one of the largest producer of cow’s milk in the world and a substantial portion of this milk is converted into dried milk products, a category that is becoming increasingly important to the dairy industry. The processing treatments on skim milk and skim milk concentrate affect the functionality of resultant powders made from them, therefore processing treatments are crucial to modify physical and functional properties of skim milk, skim milk concentrates and the powders made from them. The objective of this research was to explore impact of new processing technologies hydrodynamic cavitation (HC) and carbon dioxide (CO2) …

Evaluation Of Essential Oils (Stay Strong) For Dairy Calves, Kelly Ann Froehlich

Electronic Theses and Dissertations

Stay Strong (SS, Ralco Inc., Marshall, MN) is a blend of essential oils (EO) and prebiotic fiber technologies designed to promote immunity and stimulate appetite, helping to diminish health challenges and stresses experienced by newborn calves. Current feeding inclusion rates are unknown to achieve optimal performance in the first 8 weeks of life. The study objectives were to determine the optimal feeding inclusion rate of SS when added to milk replacer (MR) to achieve optimal performance when compared to a yeast cell wall (YCW). One hundred Holstein calves were blocked by birth date and randomly assigned to 1 of 5 …

Effect Of Direct-Fed Microbials And Enzyme Supplementation In Prepartum Holstein Cows On Colostrum And Calf Immunity, Erin M. Shangraw

Honors Theses and Capstones

In cows, colostrum is composed of several antibodies and nutrients to provide immunity and energy to the calf. Feeding calves high quality colostrum has been shown to improve calf health, leading to reduced mortality in calves and greater milk production in cows. The addition of direct-fed microbials (DFM) to cow diets has been theorized to improve feed efficiency and milk production, with studies showing mixed results. However, few experiments have studied the effect of feeding DFM on colostrum quality. In this experiment two treatments were given, 1) DFM and 2) DFM and enzymes (DFME). Colostrum was …

Incorporating Machine Vision In Precision Dairy Farming Technologies, Anthony N. Shelley

Theses and Dissertations--Electrical and Computer Engineering

The inclusion of precision dairy farming technologies in dairy operations is an area of increasing research and industry direction. Machine vision based systems are suitable for the dairy environment as they do not inhibit workflow, are capable of continuous operation, and can be fully automated. The research of this dissertation developed and tested 3 machine vision based precision dairy farming technologies tailored to the latest generation of RGB+D cameras. The first system focused on testing various imaging approaches for the potential use of machine vision for automated dairy cow feed intake monitoring. The second system focused on monitoring the gradual …

Monitoring Yogurt Culture Fermentation And Predicting Fermentation Endpoint With Fluorescence Spectroscopy, Timothey P. Mains, Frederick Alan Payne, Michael P. Sama

Biosystems and Agricultural Engineering Faculty Publications

Determination of the endpoint of yogurt culture fermentation is a process parameter that could benefit from automation. The feasibility of using a fluorescence sensor technology based on 280 nm excitation and 350 nm emission to predict the endpoint of yogurt culture fermentation was investigated and compared with the endpoint prediction from a near-infrared (880 nm) light backscatter sensor. Yogurt cultures with three levels of milk solids (8%, 10%, and 12%) and three temperatures (40°C, 43°C, and 46°C) were tested with three replications in a 3 x 3 factorial design (n = 27). Prediction models were developed for each optical measurement …

Culturally Speaking: Of Rotary Phones And Dairy Foods, Phillip Tong

Dairy Science

No abstract provided.