Dairy Science Commons^™

Genome To Phenome: Improving Animal Health, Production, And Well-Being – A New Usda Blueprint For Animal Genome Research 2018–2027, Caird Rexroad, Jeffrey Vallet, Lakshmi Kumar Matukumalli, James Reecy, Derek Bickhart, Harvey Blackburn, Mark Boggess, Hans Cheng, Archie Clutter, Noelle Cockett, Catherine Ernst, Janet E. Fulton, John Liu, Joan Lunney, Holly Neibergs, Et Al.

Animal, Dairy, and Veterinary Science Faculty Publications

In 2008, a consortium led by the Agricultural Research Service (ARS) and the National Institute for Food and Agriculture (NIFA) published the “Blueprint for USDA Efforts in Agricultural Animal Genomics 2008–2017,” which served as a guiding document for research and funding in animal genomics. In the decade that followed, many of the goals set forth in the blueprint were accomplished. However, several other goals require further research. In addition, new topics not covered in the original blueprint, which are the result of emerging technologies, require exploration. To develop a new, updated blueprint, ARS and NIFA, along with scientists in the …

Evaluation Of Casein Hydrolysate As An Alternative Dry-Off Treatment And Milk Quality Management Tool In Dairy Cows, Justine Elena Britten

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Mastitis, an infection of the mammary gland, is the most common and expensive animal health problem for the dairy industry and affects every dairy farm to some degree. This disease complex is painful for dairy cows, increases the on-farm use of antibiotics, presents a threat to milk quality and is a waste of time, money and milk production. Each year, the dairy industry loses as much as a billion dollars to mastitis.

Many cows will experience mastitis at least once during a lactation cycle and some animals will develop recurring mastitis episodes in a single mammary quarter. These mastitic quarters …

Analyzing Fear Free Veterinary Practices And Their Effectiveness In Managing Client And Patient Fear, Anxiety, And Stress, And The Profitability Of These Techniques, Heather Hamblin

Undergraduate Honors Capstone Projects

Fear-Free veterinary techniques are a new approach to caring for patients and clients in the veterinary clinic that have gained a great deal of popularity and traction. However, many veterinary professionals are left to question whether the utilization of these practices is resulting in a big enough change in client and patient stress levels to make it worth implementing these methods. This research looks into the views of veterinarians and clients on the effectiveness of these practices, and the competitive advantage of having a fear-free certified clinic from a business point of view.

Fermentation Of Prebiotics In Whole Food Powders By Probiotic Lactic-Acid Producing Bacterial Strains To Identify Synbiotic Combinations, Michaela Brubaker

Undergraduate Honors Capstone Projects

Dietary interventions with probiotic lactic acid bacteria (LAB) and prebiotics, complex dietary fibers that promote LAB growth, may favorably shift the gut microbiome to reduce colorectal cancer risk. Our primary hypothesis was that the LAB strains NCFM Lactobacillus acidophilus and HNO 19 Bifidobacterium lactis would thrive in the presence of agave, green banana, black raspberry, baobab fruit, or pomegranate peel whole food powders by fermenting their oligosaccharide (OS) components into lactic acid end products. LAB strains were cultured in media with no carbohydrate, purified OS, or one of the whole food powders. LAB strains cultured with agave appeared to grow …

Manipulation Of Ovarian Function Significantly Influenced Glucose Metabolism In Cba/J Mice, Kyleigh Ann Tyler

Undergraduate Honors Capstone Projects

Menopause is associated with a decline in overall health in women. One health aspect impacted is glucose metabolism. As women experience menopause, their metabolism declines dramatically. The current study addressed the influence of ovarian somatic cells on the improvement of metabolic health through transplantations of young, germ cell-depleted ovaries. The purpose of this study is to expand the understanding of female reproductive health on metabolism. Control mice were grouped by age and treatment mice were age-matched. Treatment mice were placed into one of three groups: 1) mice received germ cell-depleted ovaries, 2) mice received germ cell-containing ovaries, and 3) mice …

A Review Of Osteoarthritis, Madelin Brooke Session

Undergraduate Honors Capstone Projects

Osteoarthritis is a degenerative joint disease which plagues many older animals and humans. It is a disease that is characterized by the degeneration of joint cartilage, inflammation, as well as chronic pain and stiffness that results from this disorder. Unfortunately, most of the treatments for this painful and chronic disease mostly involve pain management and temporary relief strategies. These strategies usually include pain medication, non-steroidal anti-inflammatory drugs, injectable lubricants, as well as surgical techniques. However, because these treatments are just temporary fixes meant for pain management and to improve quality of life they must continue throughout the remainder of the …

Young Germ Cell Depleted Ovaries In Post-Reproductive Mice And Its Effects On Immune Function, Mckenna Walters

Undergraduate Honors Capstone Projects

It has previously been shown that young, cycling ovarian transplantation in aged female mice increased the general health and life span in regard to their post-reproductive health. It has further been hypothesized that this enhancement of health is directly influenced by the ovarian somatic cells. To address this hypothesis, transplants of young germ cell depleted and germ cell containing ovaries were performed on female mice. The purpose of this study is to continue to discern the reproductive influence on aging health, specifically in the area of immunological well-being. Control group mice were separated by age and treatment mice were subsequently …

Development Of Mouse Models For Respiratory And Neurological Disease Caused By Enterovirus D68 And Evaluation Of Antiviral Therapies, Brett L. Hurst

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Enterovirus D68 (EV-D68) is a virus that normally causes disease in children. While this virus typically causes a respiratory infection, in 2014, a large outbreak of the virus was associated with patients that had paralysis of the arms or legs. Even though the virus was discovered in 1962, little was known about the life cycle of the virus or its ability to cause disease. An animal model of disease was needed to understand how the virus causes disease and to develop antiviral compounds to target the virus life cycle.

We adapted the virus by serial-passage in lung tissues from mice …

Ruminal Protection And Intestinal Availability Of Rumen-Protected Methionine And Lysine In Lactating Dairy Cows, Sara Menchu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Rumen protected Methionine (MET) and Lysine (LYS) are critical for milk protein synthesis in dairy cows. N-acetyl-L-methionine (NALM) is a MET derivative that consists of L-Met protected with an acetyl group that is attached to the α-amino group.N-acetyl-L-lysine (NALL) is a LYS derivative that is similarly protected. The objectives of these studies were to quantify the gastrointestinal availability of NALM and NALL. Three experiments were run as 3 × 3 Latin square using 3 second lactation Holstein cows that have been fitted with cannulas in the rumen and duodenum. The cows …

Altered Gene Response To Aflatoxin B1 In The Spleens Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe Jr.

Animal, Dairy, and Veterinary Science Faculty Publications

Susceptibility and/or resistance to aflatoxin B₁ (AFB₁) is a threshold trait governed principally by glutathione S transferase (GST)-mediated detoxification. In poultry, domesticated turkeys are highly sensitive to AFB₁, most likely due to dysfunction in hepatic GSTs. In contrast, wild turkeys are comparatively resistant to aflatoxicosis due to the presence of functional hepatic GSTAs and other possible physiological and immunological interactions. The underlying genetic basis for the disparate GST function in turkeys is unknown as are the broader molecular interactions that control the systemic response. This study quantifies the effects of dietary AFB₁ on gene …

Efficacy Of A Ml336 Derivative Against Venezuelan And Eastern Equine Encephalitis Viruses, Colleen B. Jonsson, Xufeng Cao, Jasper Lee, Jon D. Gabbard, Yong-Kyu Chu, Elizabeth A. Fitzpatrick, Justin G. Julander, Dong-Hoon Chung, Jennifer Stabenow, Jennifer E. Golden

Animal, Dairy, and Veterinary Science Faculty Publications

Currently, there are no licensed human vaccines or antivirals for treatment of or prevention from infection with encephalitic alphaviruses. Because epidemics are sporadic and unpredictable, and endemic disease is common but rarely diagnosed, it is difficult to identify all populations requiring vaccination; thus, an effective post-exposure treatment method is needed to interrupt ongoing outbreaks. To address this public health need, we have continued development of ML336 to deliver a molecule with prophylactic and therapeutic potential that could be relevant for use in natural epidemics or deliberate release scenario for Venezuelan equine encephalitis virus (VEEV). We report findings from in vitro …

Quantification Of Tylosin Antibiotics In Cattle Waste, Appala Keerthi

Masters Theses & Specialist Projects

Antibiotics are used as prophylactic agents to promote growth and for treating infections in animals. However, the irrational use of antibiotics in livestock management is a significant cause of the development of antibioticresistant genes in the environment. Each year 2 million people suffer from the infections caused by bacteria which are resistant to antibiotics and 23,000 of these people are estimated to die because of antibiotic resistance. New drugs are continually coming into the market but are at the risk of developing resistance. Thus, there is a need for the development of analytical methods which can be used to monitor …

Islands In A Sea Of Aaruul: Globalization And Mongolian Cheesemakers, Pearse Anderson

Independent Study Project (ISP) Collection

Despite being a sparsely-populated country with less than 2% of its land arable, Mongolia has developed a rich and complex food culture, notably for its meat and dairy products, which could soon be at an historic high thanks to the tens of millions of animals on Mongolian pastureland. Many Mongolians and non-Mongolians view the countryside as a sea of milk that is currently being underutilized for economic exploitation. Various projects, whether funded and organized by international NGOs, the Mongolian government, or private companies, have tried to use Mongolian’s dairy resources to fill Mongolian demand, with more recent private ventures also …

Evaluating The Impact Of Feed Supplementation On Productive And Reproductive Efficiency In Smallholder Dairy Cattle In Arusha, Tanzania, Hayley Hall

Independent Study Project (ISP) Collection

This study was performed to determine if supplementing concentrates and/or minerals significantly affect productive and reproductive efficiency in smallholder dairy cattle in Arusha, Tanzania. The goal was to estimate the necessity and effectiveness of supplementing a forage diet on production and reproduction. Data was collected through interviews of small scale dairy cattle owners in nine regions within and around Arusha. Productive efficiency was measured by milk yield per cow per day. Reproductive efficiency was evaluated by the amount of time to heat resumption after calving and reproductive disease status. This was studied because determining the factors that improve the efficiency …

Mapping Of Dynamic Transcriptome Changes Associated With Silica-Triggered Autoimmune Pathogenesis In The Lupus-Prone Nzbwf1 Mouse, Melissa A. Bates, Abby D. Benninghoff, Kristen N. Gilley, Andrij Holian, Jack R. Harkema, James J. Pestka

Animal, Dairy, and Veterinary Science Faculty Publications

Crystalline silica (cSiO₂) is a widely recognized environmental trigger of autoimmune disease. In the lupus-prone female NZBWF1 mouse, airway exposure to cSiO₂ triggers pulmonary ectopic lymphoid neogenesis, systemic autoantibody elevation, and glomerulonephritis. Here we tested the hypothesis that upregulation of adaptive immune function genes in the lung precedes cSiO₂-triggering of autoimmune disease in this model. The study include three groups of mice, as follows: (1) necropsied 1 d after a single intranasal instillation of 1 mg cSiO₂ or vehicle, (2) necropsied 1 d after four weekly single instillations of 1 mg cSiO₂ or …

Fermentation Of Prebiotics In Whole Food Powders By Probiotic Bacteria Strains, Michaela Brubaker, Sumira Phatak

Research on Capitol Hill

Colorectal cancer (CRC) is the second leading cause of cancer death in the United States. Modification of the gut microbiome offers potential for CRC prevention.

Generation Of H7n9-Specific Human Polyclonal Antibodies From A Transchromosomic Goat (Caprine) System, Hua Wu, Zhiqiang Fan, Michelle Brandsrud, Qinggang Meng, Molly Bobbitt, Misha Regouski, Rusty Stott, Alexis Sweat, Jackelyn Crabtree, Robert J. Hogan, Ralph A. Tripp, Zhongde Wang, Irina A. Polejaeva, Eddie J. Sullivan

Animal, Dairy, and Veterinary Science Faculty Publications

To address the unmet needs for human polyclonal antibodies both as therapeutics and diagnostic reagents, building upon our previously established transchromosomic (Tc) cattle platform, we report herein the development of a Tc goat system expressing human polyclonal antibodies in their sera. In the Tc goat system, a human artificial chromosome (HAC) comprising the entire human immunoglobulin (Ig) gene repertoire in the germline configuration was introduced into the genetic makeup of the domestic goat. We achieved this by transferring the HAC into goat fetal fibroblast cells followed by somatic cell nuclear transfer for Tc goat production. Gene and protein expression analyses …

Human Polyclonal Antibodies Produced From Transchromosomal Bovine Provides Prophylactic And Therapeutic Protections Against Zika Virus Infection In Stat2 Ko Syrian Hamsters, Venkatraman Siddharthan, Jinxin Miao, Arnaud J. Van Wettere, Rong Li, Hua Wu, Eddie Sullivan, Jinan Jiao, Jay W. Hooper, David Safronetz, John D. Morrey, Justin G. Julander, Zhongde Wang

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) infection can cause severe congenital diseases, such as microcephaly, ocular defects and arthrogryposis in fetuses, and Guillain–Barré syndrome in adults. Efficacious therapeutic treatments for infected patients, as well as prophylactic treatments to prevent new infections are needed for combating ZIKV infection. Here, we report that ZIKV-specific human polyclonal antibodies (SAB-155), elicited in transchromosomal bovine (TcB), provide significant protection from infection by ZIKV in STAT2 knockout (KO) golden Syrian hamsters both prophylactically and therapeutically. These antibodies also prevent testicular lesions in this hamster model. Our data indicate that antibody-mediated immunotherapy is effective in treating ZIKV infection. Because suitable …

Differential Transcriptome Responses To Aflatoxin B1 In The Cecal Tonsil Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe Jr.

Animal, Dairy, and Veterinary Science Faculty Publications

The nearly-ubiquitous food and feed-borne mycotoxin aflatoxin B₁ (AFB₁) is carcinogenic and mutagenic, posing a food safety threat to humans and animals. One of the most susceptible animal species known and thus a good model for characterizing toxicological pathways, is the domesticated turkey (DT), a condition likely due, at least in part, to deficient hepatic AFB₁-detoxifying alpha-class glutathione S-transferases (GSTAs). Conversely, wild turkeys (Eastern wild, EW) are relatively resistant to the hepatotoxic, hepatocarcinogenic and immunosuppressive effects of AFB₁ owing to functional gene expression and presence of functional hepatic GSTAs. This study was designed to …

Jackrabbit Dairy And Food Review, Vikram V. Mistry

The Jackrabbit Dairy & Food Review

This is the Winter 2017- 2018 Jackrabbit Dairy and Food Review. It contains the following articles and information: Message from the Department Head, 2017 Graduates, David A. Thompson Endowment, New Faculty and Visitors, information on the Dairy Club and Food Science Club, Judging Activities, Student Accomplishments, Professional Activities of Faculty and Staff, Distinguished Alumni, Dairy Facilities, a list of Scholarships and Dairy and Food Science Students, information on Departmental Outreach and Research Highlights.

Perceptions Of On-Farm Emergency Slaughter For Dairy Cows In British Columbia, Katherine E. Koralesky, David Fraser

Slaughter and Slaughtering Practices Collection

Some jurisdictions permit on-farm emergency slaughter (OFES) as one end-of-life option for dairy cows and other animals that cannot be transported humanely but are deemed fit for human consumption. Anecdotal reports suggest that OFES is controversial among dairy industry professionals, but to date their perceptions of OFES have not been studied systematically. Twentyfive individual interviews and 3 focus groups with 40 dairy producers, veterinarians, and other professionals in British Columbia, Canada, revealed positive and negative perceptions of OFES influenced by (1) individual values, (2) the perceived operational legitimacy of OFES, and (3) concern over social responsibility and public perception of …

Using An Implantable Microchip For Measuring Body Temperature In Dairy Calves, Megan Woodrum, Melissa Cantor, Joao H. C. Costa

Posters-at-the-Capitol Presentations

Body temperature is frequently used as a method for determining if illness is present with fever detection. Taking rectal temperature or alternatively tympanic temperature can be time consuming and requires restraint of calves. Alternatives, such as implantable microchips that can be passively read using a radio frequency identification (RFID) scanner may allow for easier monitoring of body temperature.

This study aim was to validate an implantable microchip and to determine the best implant site.

Modelling And Simulation Of Renewable Technologies For Economic And Environmental Analysis And Optimization Of Dairy Farms, Michael Christopher Breen

Theses

The aim of this thesis was to develop a dairy optimisation platform to optimise investments in dairy farm technology and changes to on-farm management practices and electricity tariffs, in order to maximise profitability and minimise environmental impacts. Increased dairy herd sizes and milk production levels in Ireland as well as changing regulations concerning emissions, renewable penetration and energy efficiency have necessitated a means of financial decision support for farmers. Therefore this body of work focused on the creation of a comprehensive method for optimising dairy farm technology selection, management practices and electricity tariffs. The measures which could be implemented on …

Hydrodynamic Cavitation As In-Line Process To Control Common Dairy Sporeformers, Pratibha Chaudhary

Electronic Theses and Dissertations

Sporeforming bacteria and their endospores have the ability to survive thermal treatments and proliferate in the milk and dairy products to cause spoilages. We applied the effect of hydrodynamic cavitation in controlling these bacteria and their spores at pilot scale level. Our aim was to investigate a technology that would improve the microbial quality of milk and dairy products, and is capable of being scaled up to be adapted at industrial level. Cavitation damages the cell walls of the bacteria and their endospores (releasing dipicolinic acid, responsible for endospore resistance to extreme conditions), thereby making them susceptible to thermal treatments …

Evaluation Of Feeding Alternative Feedstuffs Including Hydroponic Barley Sprouts And Carinata Meal To Dairy Cattle, Rhea D. Lawrence

Electronic Theses and Dissertations

The purpose of this dissertation research was to examine alternative feed ingredients not typically found in dairy cattle diets. In total, four studies were conducted to evaluate feedstuffs such as hydroponic barley sprouts and carinata meal and how they affect cattle performance. To determine how feeding hydroponic barley sprouts would affect growing dairy heifer and lactating cow performance two feeding studies were conducted. In the first study, inclusion of 14 % (DM basis) hydroponic barley sprouts (HYD) was evaluated in an ad libitum total mixed ration (TMR) compared to a control (CON) diet on dairy heifer performance during a 12 …

Evaluation Of Effects Of Methods Used For Forage Analysis And Dietary Inclusion Of Buffer On Fiber Digestibility And Rumen Fermentation In Diets High In Distillers Grains, Lydia Kehinde Olagunju

Electronic Theses and Dissertations

Forage quality is an important factor affecting intake and utilization of forage, thus making it imperative to evaluate the methods used to determine the nutritive values of forage in order to predict animal performance. Additionally, fibrous feeds for ruminants are less subject to competitive demand. The plant cell wall is the largest hindrance to complete digestion of feeds, particularly forages and by-products and to the utilization of the nutrients and energy they contain, necessitating effective strategies for increasing the rate and efficiency of utilization of forage fiber and the energy therein. It is critically important to increase fiber digestion for …

Evaluation Of Flax Oil Supplementation On Performance Of Growing Dairy Heifer Calves And Lactating Cows, Chelsea Rose Schossow

Electronic Theses and Dissertations

Utilization of flax oil is new to the livestock industry, and limited research has been done to see the benefits of feeding the highly unsaturated oil to both dairy calves and lactating cows. Two studies were conducted to evaluate the potential benefits of flax oil as a lipid supplement. The first study compared the supplementation of two plant based oils to growing dairy calves. Thirty-six female Holstein calves in individual hutches were used in a 12-wk randomized complete block design study. Treatments were: 1) control (CON) with no oil, 2) 80 g/d of flax oil (FLAX), and 3) and 80 …

Assessing Listeria Risk During Different Stages Of Ice Cream Manufacturing And Storage, Neha Singh

Electronic Theses and Dissertations

Listeriosis is a life-threatening infection caused by foods contaminated with Listeria monocytogenes. Some of the major ice cream recalls in recent years reaffirm the ability of this food-borne pathogen to survive in diverse dairy processing environments and cause cross contamination. Inspection reports revealed certain lapses in implementing adequate hygienic practices for Listeria persistence in the processing environment, leading to cross contamination of ice cream. The higher levels of cross contamination of raw ice cream mix might result in random heat-injured cells when exposed to minimum heat treatment (69°C for 30 min). Evidence about the presence of injured cells in ice …

Novel Approaches To Evaluate And Enhance Neonatal Calf Gastrointestinal Health And Development, Fernanda Trindade Da Rosa

Electronic Theses and Dissertations

Physiological adaptations of the gastrointestinal tract (GIT) epithelial cells as well as methods to manipulate early life GIT microbiota colonization during the neonatal stage, is of great importance to the dairy industry. The first objective of this research was to optimize a method based on evaluation of bovine transcripts in fecal RNA via RTqPCR using L-selectin (SELL) as a marker for polymorphonuclear leukocytes (PMNL), keratin 8 (KRT8) and fatty acid binding protein 2 (FABP2) for GIT enterocytes, and tight junctions in fecal RNA and GIT sections in dairy calves. To test the reliability of the fecal RNA method, fecal and …

Effects Of Yeast-Derived Microbial Protein On Transition Dairy Cow Health And Performance, Gustavo Mazon Correa Alves

Theses and Dissertations--Animal and Food Sciences

The transition period for dairy cows is defined as the three weeks pre and postpartum. During the transition period, dairy cows experience a myriad of metabolic, managerial, and nutritional requirement changes. These changes lead to stress and increased susceptibility to diseases which can negatively affect lactational performance in the short and long term. However, dietary amino acid availability can have a dramatic impact on the health and performance of dairy cows around parturition. Thus, the objective of the thesis was to evaluate the effects of supplementing yeast-derived microbial protein, as an alternative protein source for dairy cows during the transition …