Engineering Commons^™

Resolving The Nanoscale Structure Of Β-Sheet Peptide Self-Assemblies Using Single-Molecule Orientation–Localization Microscopy, Weiyan Zhou, Conor L. O'Neill, Tianben Ding, Oumeng Zhang, Jai S. Rudra, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

Synthetic peptides that self-assemble into cross-β fibrils are versatile building blocks for engineered biomaterials due to their modularity and biocompatibility, but their structural and morphological similarities to amyloid species have been a long-standing concern for their translation. Further, their polymorphs are difficult to characterize using spectroscopic and imaging techniques that rely on ensemble averaging to achieve high resolution. Here, we utilize Nile red (NR), an amyloidophilic fluorogenic probe, and single-molecule orientation-localization microscopy (SMOLM) to characterize fibrils formed by the designed amphipathic enantiomers KFE8^L and KFE8^D and the pathological amyloid-beta peptide Aβ42. Importantly, NR SMOLM reveals the helical (bilayer) …

Developing The Next Indiana Mutcd, David Boruff

Purdue Road School

The session will provide a brief overview of the updates to the new 11th edition of the MUTCD that would be of interest to local agencies.

Stop Doing Schedules Wrong!, Bernard Nartey

Purdue Road School

This session will unveil the common errors, acts of non-compliance, and oversights in construction project scheduling and their impact on the management of the contract.

Purple Line Bus Rapid Transit Project Overview, Michael Schneider, Rachel Wilson

Purdue Road School

The Purple Line will be Indianapolis’ second Bus Rapid Transit (BRT) Line, opening for revenue service in the fall of 2024. It will provide rapid transit service along 15.2 miles of E. 38th Street and Post Road, between Indianapolis and Lawrence, for a total cost of $188 million. This presentation will provide an overview of IndyGo’s overall capital program with a focus on the Purple Line project—its unique design features and successful project delivery—from design through construction.

Indiana Design Manual Chapter 46 Updates, Nathaniel Shellhamer, Taylor Ruble

Purdue Road School

INDOT is working on a total revision of Chapter 46 “Intersections at Grade” of the Indiana Design Manual (IDM). These revisions address numerous longstanding concerns with the way intersections are designed in Indiana and also contribute design information for alternative intersections.

Public Speaking Level 2: The Skill Of Storytelling And Persuasion, Tim Jensen

Purdue Road School

Building on “Public Speaking for Engineers and Public Officials,” this session will take it to the next level of public speaking. We will discuss and build skills of storytelling and persuasion and how it relates to people and public speaking.

Introduction Into Land Aquisition, James Hellmann, Jonathan Hughes

Purdue Road School

This discussion will give an overview of the laws on land acquisition, what the typical process looks like, and one community’s perspective on communication related to the process.

Consultant Contracts Process Improvement, Joel Myers, Gretchen West

Purdue Road School

Are you curious about what happens after RFP selections are released or what happens after a proposal is submitted? The INDOT consultant contracts section completed a deep dive into identifying process improvements. This session breaks down our approach to process improvement review, implemented improvements, improvements that are planned, and an overview of lessons learned.

Connecting Tsmo To The Tmc To Motorists, Drew Sorenson, John Lablonde

Purdue Road School

Join us as we connect the exciting world of Transportation System Management and Operations (TSMO) along I-465 in southeast Indianapolis to the Traffic Management Center (TMC) and the traveling public. We will share how the TSMO strategies of ramp metering and variable speed limit signs are connected and monitored at the INDOT TMC in real-time and how that information goes to the traveling public to improve safety and mobility on Indy’s southeast side.

Exploration And Implementation Of Autonomous Truck Mounted Attenuator (Atma) In Indiana Roadway Maintenance Activities, Yunfeng Chen, Yiheng Feng, Jiansong Zhang

Purdue Road School

INDOT reported 153 truck mounted attenuator (TMA)-related accidents between 2016 and 2021. The autonomous TMA (ATMA) removes the driver from the TMA to enhance their safety; however, the functionality and workers’ perception of ATMA must be assessed comprehensively before its implementation in INDOT. This presentation summarizes the findings from 25 closed- road tests and five weeks of field tests of ATMA under four maintenance activities.

Utility Construction Headaches, Aaron Day, Brandi Redd, Aj Wortkoetter

Purdue Road School

This presentation will discuss services points , grant charts, master relocation plans and utility effects on construction.

County Bridge Preservation: Locally Funded Vs. Federally Funded, Kevin Russel, Nick Parr, Jonathan Olson

Purdue Road School

This presentation will discuss the previous federal aid call for bridge preventative maintenance projects, the INDOT Asset Management Plan format vs. CCMG, how the federal aid call was cancelled, and how Harrison County and Boone County moved projects forward with 100% local funds. We will also explore how far counties have come on asset management with pavements, and the continuing need adopt preventative maintenance on bridges. We will conclude with an overview of how these projects can be delivered locally vs. with federal funds.

It's Electric!: Ev Planning In Your Community, Cheryl Sharp, Stacy Milheiser

Purdue Road School

Planning for EV charging stations is more than under- standing new technology. It’s amending your community’s guiding documents, performing site selection and feasibility, future-proofing and contingency planning, and understanding the financial considerations and available funding opportunities. Using our knowledge gained through our EVCS municipal planning experience, you’ll learn the steps needed to prepare your community for the EV wave and to best situate yourself for successful grant applications.

Mse Walls Retrofit Design And Construction, Aamir Turk, Malek Smadi

Purdue Road School

Presenters will share the INDOT MSE wall retrofit contracting approach, design, and construction for existing MSE walls that showed signs of significant premature distresses.

Demystifying Traffic Engineering For The Rest Of Us, Nathaniel Shellhamer, David Reamer

Purdue Road School

Have you ever uttered the phrase “I’m not a traffic engineer”? When traffic engineers go off on a tangent, can it start to sound like a foreign language? This session is for you! We will break down traffic engineering concepts, such as innovative intersections, traffic analysis buzzwords and acronyms, and more. This session will be geared towards non-traffic engineers and anyone else who might explain these concepts to the traveling public.

Reimagining Cherry Lane: A Road To Collaboration, Adam Keyster, Ben Anderson

Purdue Road School

Join us on a case study to learn how collaborative efforts between Purdue University and the City of West Lafayette created multimodal infrastructure through the realignment of Cherry Lane and supporting work. Presenters will discuss how this project is solving longstanding issues that the city and university have encountered over the years and how the community benefits from the implemented solution.

Demystifying Utility Coordination: Understanding The Basics, Josshe I. Palm

Purdue Road School

This session will explain utility coordination to non-utility coordinators. It will help educate others about the role and responsibilities of a utility coordinator, an OSA, easy UC and design mistakes to avoid, scope and design considerations, items commonly overlooked, when to coordinate SUE, and how to work with your UC to request a letting adjustment. We will also highlight UC submittals and allow time for questions about the UC’s role in a project’s success.

It Starts With You: Effective Team Building Strategies, Karen Wood, Jennifer L. Sharkey

Purdue Road School

This presentation will provide strategies to build effective teams. Transportation projects require effective collaboration, which is accomplished through team building and trust. To successfully manage and work in a team made up of diverse people with different perspectives and experiences, we must first look inward to build self-awareness and emotional intelligence. In doing so, we can connect with others by being our authentic selves and listening to understand.

The Role Of The Owner’S Representative In Public Projects, Tim Jensen, Scott Willis

Purdue Road School

This presentation will explain how an owner’s representative works with the municipality to deliver quality projects on behalf of the community.

Indianapolis White River Innovation District: Bot Success Story, Cassie Reiter, John Bowen, Shannon Killion

Purdue Road School

When Elanco announced their headquarters will be at the former GM stamping plant site in Indianapolis, Indy DPW, IEDC and their partners needed to not only design roadway infrastructure, but also all-new utility infrastructure and a new bridge across the White River. When the build-operate-transfer procurement process became available for use by Indianapolis, the design teams and owners embraced the opportunity for a collaborative construction process on this transformative project.

Stormwater Management In Flat Floodplains, Kari Mackenbach

Purdue Road School

Communities across the Midwest have experienced chronic stormwater drainage and increased flooding of roadways and structures. While adequate storm drainage seems to be lacking in many rural communities, steps can be taken to address some of the nuisance flooding that plagues many regions. Walnut Township commissioned a stormwater study that included the development of a 2D model to understand and mitigate flooding in their area.

Applied Ergonomics, Yunfeng Chen, Jiansong Zhang

Purdue Road School

There are approximately 2.3 million work-related injuries and over 6,000 fatalities every year. In 2020, half of these injuries happened in the construction industry. Our research explored the three main causes of work-related injuries, identified high-risk activities and the root causes of injuries in those activities, evaluated current safety practices in INDOT, proposed ergonomically effective technologies, and conducted experiments to evaluate if those ergonomic interventions can improve workers’ health and safety. Join us for a discussion.

Us 33 Over Blue River: Abc’S With Steel, Brandon Arnold, Kurt Heidenreich, Stephanie Wagner

Purdue Road School

The US 33 over the Blue River Bridge Replacement project is the first INDOT project of its kind, and a great example of Accelerated Bridge Construction (ABC). The project used prefabricated steel beam modular super- structure units with prefabricated substructure units, approach slabs, terminal joints, and “steel-like” UHPC for the 8-inch closure pours. Correcting the substan- dard roadway superelevation added complexity to the design and detailing. Join us to learn more.

Tips For Successful Lpa Engineers & Supervisors, Michael M. Gavelek, Jennifer L. Sharkey

Purdue Road School

The session will present lessons learned, tips, tricks, and lifehacks for LPA staff. We’ll unpack common traffic requests, HMA inlay and construction, nuggets from a long-lost LPA manual, and a long-range asset management strategy. resources. We’ll share valuable resources throughout the presentation that will save you time and money and position you to successfully serve your LPA.

Engineering Without Borders Usa, Katherine Kreienkamp, Allen Boyd, Susan Harris

Purdue Road School

Join us for an informative presentation about the mission, sustainable design practices, needs, and opportunities of the EWB (engineers without borders). This 17,000-member organization addresses clean water, sanitation, and other core living needs of small, disadvantaged international communities. EWB conducts hundreds of global projects per year and offers significant volunteer opportunities for civil, environmental, and electrical engineers and mentoring opportunities for scores of EWB student chapters.

Spatiotemporal Reaction Dynamics Control In Two-Photon Polymerization For Enhancing Writing Characteristics, Aofei Mao, Sarah Fess, Nada Kraiem, P. Li, Zhipeng P. Wu, Qiuchi Zhu, Xi Huang, Peixun Fan, Bai Cui, Jean-Francois Silvain, Suxing Hu, Mitchell Anthamatten, Sean P. Regan, David Harding, Yongfeng Lu

Department of Electrical and Computer Engineering: Faculty Publications

Since 2001, 3D microfabrication based on two-photon polymerization (TPP) has drawn extensive attention and interest in biology, optics, photonics, material science, and high-energy physics. The in-volume fabrication capability due to the threshold behavior of two-photon absorption enables TPP higher flexibility compared with other nanofabrication techniques. However, as determined by the in-volume fabrication feature as well as various reaction dynamics, the writing characteristics of TPP, such as throughput, accuracy, surface quality, and fabrication capability, are still limited. Herein, a comprehensive study is performed on the spatiotemporal behavior of reaction dynamics during TPP fabrication, mainly focusing on spatiotemporal characteristics of radical diffusion, …

Single And Dual Cell Encapsulation Via Liquid Core Hydrogels Using Pico Injection, Jade Houston

USF Tampa Graduate Theses and Dissertations

In this project, a comprehensive microfluidic device, along with several interdependent subsystems, was designed and developed in SolidWorks, leveraging a combination of 3D resin printing with biocompatible resin and micromachining with polymethylmethacrylate (PMMA). The microfluidics chip integrated both passive and active components to facilitate its primary function: dual cell encapsulation via liquid core sodium alginate hydrogel droplets.

Integral to the microfluidics chip design were key features including a 5-convolution Archimedes spiral for particle sorting, a flow focusing junction for droplet generation, and a pico injector for injecting additional cells into passing droplets via the manipulation of an electric field.

An …

Development Of A Plant Growth And Health Monitoring System Using Imaging And Sensor Array Information For Cubesat Applications, Kat-Kim Phan

USF Tampa Graduate Theses and Dissertations

Space exploration has been a topic of interest in the scientific community, such as the planned missions to Mars. To accomplish this would require being able to provide astronauts with a steady supply of food beyond freeze-dried foods leading to the need to grow food in space. Although this is a topic still being investigated, the CubeSat platform opens the possibility of carrying out studies on plant growth under more strenuous space conditions unlike that in the International Space Station (ISS). Developing a plant-focused mission for a CubeSat, though, entails being able to develop a system to sustain plant life …

Comparison Of Conventional And Adaptive Acoustic Beamforming Algorithms Using A Tetrahedral Microphone Array In Noisy Environments, Megan Brittany Ewers

Dissertations and Theses

In situ acoustic measurements are often plagued by interfering sound sources that occur within the measurement environment. Both adaptive and conventional beamforming algorithms, when applied to the outputs of a microphone array arranged in a tetrahedral geometry, are able to capture sound sources in desired directions and reject sound from unwanted directions. Adaptive algorithms may be able to measure a desired sound source with greater spatial precision, but require more calculations and, therefore, computational power. A conventional frequency-domain phase-shift algorithm and a modified adaptive frequency-domain Minimum Variance Distortionless Response (MVDR) algorithm were applied to simulated and recorded signals from a …

6d Single-Fluorogen Orientation-Localization Microscopy For Elucidating The Architecture Of Beta-Sheet Assemblies And Biomolecular Condensates, Tingting Wu, Weiyan Zhou, Jai S. Rudra, Rohit V. Pappu, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

We develop six-dimensional single-molecule orientation-localization microscopy (SMOLM) to measure the 3D positions and 3D orientations simultaneously of single fluorophores. We show how careful optimization of phase and polarization modulation components can encode phase, polarization, and angular spectrum information from each fluorescence photon into a microscope’s dipole-spread function. We used the transient binding and blinking of Nile red (NR) to characterize the helical structure of fibrils formed by designed amphipathic peptides, KFE8^L and KFE8^D, and the pathological amyloid-beta peptide Aβ42. We also deployed merocyanine 540 to uncover the interfacial architectures of biomolecular condensates.