Stem Cell Derived Osteoprogenitors And Their Role In Bone Repair Using Morphogenic Activators, 2017 Dominican University of California
Stem Cell Derived Osteoprogenitors And Their Role In Bone Repair Using Morphogenic Activators, Angelina Miller, Sylvanna Islas, George Washington Brownridge Iii, Warren Hoeffler
Scholarly & Creative Works Conference 2017
Bone constantly cycles through a dynamic process of breakdown and remodeling. Osteoblasts are the specialized mesenchymal stem cells that have a major role in bone formation and the remodeling process whereas their counterpart osteoclasts, handle bone resorption. Embryonic stem cells can be partially differentiated into Progenitor cells, and we worked with #18, a candidate for being an osteoprogenitor that has the potential to respond to morphogenic activators. In the case of bone remodeling, TGF-β 2, BMP-2 and an abundance of CA++ have been shown to be potential activators of differentiation into osteoblasts. Eight different trials were conducted with the cells ...
The Effect Of Auditory Stimulation On Human Movement Variability And Associated Cortical Involvement, 2017 Biomechanics Research Building
The Effect Of Auditory Stimulation On Human Movement Variability And Associated Cortical Involvement, Samuel Maaiah 9051573
Student Research and Creative Activity Fair
The Biomechanics Research Building (BRB) is presently using functional near-infrared spectroscopy (fNIRS) to measure and understand brain activity during human movement and exercise. Compared to other brain activity measuring methods, which require subjects to remain very still during recording, fNIRS allows researchers to study brain activity while subjects perform a range of movement tasks. It is consequently a useful tool for studying movements (e.g. walking) that are not conducive to conventional methods of measuring brain activity. Experiments using fNIRS can vary dramatically in duration depending upon the research question. Some experimental methods contain multiple trials for short time intervals ...
Modelling Random Antibody Adsorption And Immunoassay Activity, 2016 Dublin Institute of Technology
Modelling Random Antibody Adsorption And Immunoassay Activity, Dana Mackey, Eilis Kelly, Robert Nooney
One of the primary considerations in immunoassay design is optimizing the concentration of capture antibody in order to achieve maximal antigen binding and, subsequently, improved sensitivity and limit of detection. Many immunoassay technologies involve immobilization of the antibody to solid surfaces. Antibodies are large molecules in which the position and accessibility of the antigen-binding site depend on their orientation and packing density. In this paper we propose a simple mathematical model, based on the theory known as random sequential adsorption (RSA), in order to calculate how the concentration of correctly oriented antibodies (active site exposed for subsequent reactions) evolves during ...
Targeted And Controlled Anticancer Drug Delivery And Release With Magnetoelectric Nanoparticles, 2016 Florida International University
Targeted And Controlled Anticancer Drug Delivery And Release With Magnetoelectric Nanoparticles, Alexandra Rodzinski
FIU Electronic Theses and Dissertations
A major challenge of cancer treatment is successful discrimination of cancer cells from healthy cells. Nanotechnology offers multiple venues for efficient cancer targeting. Magnetoelectric nanoparticles (MENs) are a novel, multifaceted, physics-based cancer treatment platform that enables high specificity cancer targeting and externally controlled loaded drug release. The unique magnetoelectric coupling of MENs allows them to convert externally applied magnetic fields into intrinsic electric signals, which allows MENs to both be drawn magnetically towards the cancer site and to electrically interface with cancer cells. Once internalized, the MEN payload release can be externally triggered with a magnetic field. MENs uniquely allow ...
Cardiomems™ In Lvad Patients: A Case Series, 2016 Gill Heart Institute, University of Kentucky
Cardiomems™ In Lvad Patients: A Case Series, Maya Guglin, Bennet George, Sarah Branam, Amanda Hart
The VAD Journal
Patients with a left ventricular assist device (LVAD) commonly encounter issues with volume status post-implant. Volume overload can result from incomplete compensation of left ventricular failure or from right ventricular failure. The CardioMEMS™ intracardiac hemodynamic monitoring device is an area of growing interest regarding the management of chronic congestive heart failure, however, its utility has not been serially investigated in patients with an LVAD. We present a case series of patients with ventricular assist devices care for at our institution in which the CardioMEMS™ device aided in the management of volume status and pump performance.
Selecting Medical Hardware Using Pairwise Comparisons: A Patient's Perspective Of Cochlear Implant Device Selection, 2016 Portland State University
Selecting Medical Hardware Using Pairwise Comparisons: A Patient's Perspective Of Cochlear Implant Device Selection, Timothy R. Anderson, Shabnam Razeghian Jahromi
Engineering and Technology Management Faculty Publications and Presentations
Selecting medical hardware can be a difficult and permanent decision for patients that they are often unprepared for. The authors explore the use of pairwise comparison techniques to better inform medical decision making in an application of choosing between three major cochlear implant manufacturers. This paper appears to be the first study to apply a pairwise comparison decision making approach for cochlear implant device selection. Also, unlike many medical decision making studies that are developed by the healthcare professional, this model and analysis was conducted entirely by the patient for the purpose of making the real-world decision of a device ...
Rnai Nanotechnology: A Platform For Sirna Screening And Cancer Gene Therapy, 2016 Seton Hall University
Rnai Nanotechnology: A Platform For Sirna Screening And Cancer Gene Therapy, Mayurbhai Ravikant Patel
Seton Hall University Dissertations and Theses (ETDs)
Over the past two decades, advances in RNA structural biology have improved our understanding of the structures and folding properties of naturally occurring RNAs. RNA sequences and structures participate in many specific biological functions, such as those performed by messenger RNA (mRNA), ribosomal RNA (rRNA), transfer RNA (tRNA), micro RNA (miRNA), short-interfering RNA (siRNA), small nuclear RNA (snRNA) and many others. The noncoding RNAs, such as siRNA, do not express proteins but have been utilized in a wide range of applications, including RNA interference (RNAi) and the regulation of mRNA expression. These important biological functions have been implemented in gene ...
Cartilage Engineering: Optimization Of Media For Chondrogenic Differentiation In Vitro, 2016 Purdue University
Cartilage Engineering: Optimization Of Media For Chondrogenic Differentiation In Vitro, Evan Surma, Sherry L. Harbin, Hongji Zhang, Stacy Halum
The Summer Undergraduate Research Fellowship (SURF) Symposium
Lower back pain from intervertebral disc injury affects around 84% of the population at some point in their life, which at its worst may cause total immobilization. This pain can only be temporarily relieved by spinal fusion or intervertebral disc replacement; however, both of these cause loss of natural motion in patients by removing damaged fibrocartilage discs. While these techniques help mitigate pain briefly, no permanent solution exists currently to both relieve pain and preserve natural motion. My work may be a solution by eventually providing patient-specific implants that resemble native tissue in the regeneration process that could be absorbed ...
Preparation Of Myod Mrna For The Differentiation Of Stem Cells Into Muscle Cells, 2016 The University of Southern Mississippi
Preparation Of Myod Mrna For The Differentiation Of Stem Cells Into Muscle Cells, Stephanie A. Ye
Embryonic stem cells (ESCs) are characterized by their two special properties: pluripotency and self-renewal. Pluripotency is the ability of ESCs to differentiate into any cell type upon expression of specific proteins called transcription factors. In order to induce differentiation, transcription factors specific for the particular cell type have to be introduced and expressed in the ESCs. The introduction of transcription factors can be achieved by using either DNA, RNA, or protein. According to the Central Dogma of Molecular Biology, DNA is first transcribed into messenger RNA (mRNA), which is then translated to make a functional protein molecule. Among the available ...
Review Of "The Human Embryonic Stem Cell Debate: Science, Ethics, And Public Policy (Basic Bioethics)," Edited By Suzanne Holland, Karen Lebacqz, And Laurie Zoloth, James Steele
University of New Hampshire Law Review
[Excerpt] "Perhaps like others, I started The Human Embryonic Stem Cell Debate not knowing many of the finer points of embryonic stem cell research, but I certainly had an opinion. This issue is far more complex than I had initially imagined. The editors do a commendable effort of compiling a sample of the innumerable arguments surrounding the debate."
Structure-Activity Relationships Governing The Interaction Of Nanoparticles With Mammalian Cells- Predictive Models For Toxicology And Medical Appliances, Marcus A. Maher
Nanoscience is seen as one of the key enabling technologies of the 20th century and as its range of applications increases it is important to look at how nanomaterials interact with biological environments. Some of these interactions have given rise to toxic effects and thus, the creation of the field of nanotoxicology, it has also been noted that current methods of evaluating toxicity may not be sufficient to keep up with the rapidly emerging range of nanomaterials becoming available. It is clear that alternatives are necessary. In this thesis, a phenomenological rate equation model is constructed to simulate nanoparticle uptake ...
Modeling The Structural Consequences Of Best1 Missense Mutations, 2016 University of Pennsylvania
Modeling The Structural Consequences Of Best1 Missense Mutations, Karina Guziewicz, Gustavo Aguirre, Barbara Zangerl
Gustavo D. Aguirre, VMD, PhD
Mutations in the bestrophin-1 gene (BEST1) are an important cause of inherited retinal disorders. Hitherto, over 100 unique allelic variants have been linked to the human BEST1 (hBEST1), and associated with disease phenotypes, broadly termed as bestrophinopathies. A spontaneous animal model recapitulating BEST1-related phenotypes, canine multifocal retinopathy (cmr), is caused by mutations in the canine gene ortholog (cBEST1). We have recently characterized molecular consequences of cmr, demonstrating defective protein trafficking as a result of G161D (cmr2) mutation. To further investigate the pathological effects of BEST1 missense mutations, canine and human peptide fragments derived from the protein sequence have been studied ...
Rpgrip1 And Cone-Rod Dystrophy In Dogs, 2016 University of Pennsylvania
Rpgrip1 And Cone-Rod Dystrophy In Dogs, Tatyana Kuznetsova, Barbara Zangerl, Gustavo Aguirre
Gustavo D. Aguirre, VMD, PhD
Cone–rod dystrophies (crd) represent a group of progressive inherited blinding diseases characterized by primary dysfunction and loss of cone photoreceptors accompanying or preceding rod death. Recessive crd type 1 was described in dogs associated with an RPGRIP1 exon 2 mutation, but with lack of complete concordance between genotype and phenotype. This review highlights role of the RPGRIP1, a component of complex protein networks, and its function in the primary cilium, and discusses the potential mechanisms of genotype–phenotype discordance observed in dogs with the RPGRIP1 mutation.
Targeting Oncogenic Mirnas With Small Molecules For Breast Cancer Therapy, 2015 The University of Texas Graduate School of Biomedical Sciences at Houston
Targeting Oncogenic Mirnas With Small Molecules For Breast Cancer Therapy, Paloma Del C. Monroig
UT GSBS Dissertations and Theses (Open Access)
The crucial role of microRNAs (miRNAs) in cancer pathobiology has driven the introduction of new drug development approaches such as miRNA inhibition. In order to advance miRNA-therapeutics, there is a need to develop screening strategies that can target tumors in a specific way. Small molecule inhibitors represent an attractive approach to pursue this. However, the absence of molecular structures for most of the miRNAs makes it very difficult to predict which inhibitors can bind to them. Herein we designed a strategy to screen for small molecules by assesing whether they could directly bind/ interact with miR-10b/miR-21. As part of ...
Organometallic Rhenium Dyes For Nitric Oxide Detection And Imaging, 2015 Florida International University
Organometallic Rhenium Dyes For Nitric Oxide Detection And Imaging, Lissette I. Lozano-Lewis
FIU Electronic Theses and Dissertations
The importance of sensing Nitric Oxide (NO) in physiology and medicine has led us to explore the reactivity of NO with organometallic Re dyes. Rhenium complexes were synthesized with the ability to react with NO and sense it under physiological conditions. Fluorescent 1,10-phenantroline complexes (phen)Re(PPh3)(CO)2OSO2CF3 (1) and (phen)Re(CH3CN)(CO)2OSO2CF3 (3) can sense NO in the range of 10 - 150 mM showing a decrease in fluorescence response at 514 nm and 532 nm respectively, upon NO-donor addition (lexc = 360 nm). (phen ...
Subclavian Vein Stenosis/Occlusion Following Transvenous Cardiac Pacemaker And Defibrillator Implantation: Incidence, Pathophysiology And Current Management, Brian O'Leary, Suhail Allaqaband
Journal of Patient-Centered Research and Reviews
Subclavian vein stenosis is a common, but usually asymptomatic, complication following cardiac device placement. In addition to reviewing the literature on incidence, pathogenesis and management options for this important clinical problem, we describe two cases of symptomatic subclavian vein occlusion following pacemaker/defibrillator placement and successful treatment with venoplasty and stenting.
The B Subunit Of Escherichia Coli Heat-Labile Toxin Alters The Development And Antigen-Presenting Capacity Of Dendritic Cells, 2015 Australian National University
The B Subunit Of Escherichia Coli Heat-Labile Toxin Alters The Development And Antigen-Presenting Capacity Of Dendritic Cells, Jing Ji, Kristin Grifftiths, Peter Milburn, Timothy Hirst, Helen O'Neill
Escherichia coli's heat-labile enterotoxin (Etx) and its non-toxic B subunit (EtxB) have been characterized as adjuvants capable of enhancing T cell responses to co-administered antigen. Here, we investigate the direct effect of intravenously administered EtxB on the size of the dendritic and myeloid cell populations in spleen. EtxB treatment appears to enhance the development and turnover of dendritic and myeloid cells from precursors within the spleen. EtxB treatment also gives a dendritic cell (DC) population with higher viability and lower activation status based on the reduced expression of MHC-II, CD80 and CD86. In this respect, the in vivo effect ...
Biodegradable Nano-Hybrid Polymer Composite Networks For Regulating Cellular Behavior, 2015 University of Tennessee - Knoxville
Biodegradable Nano-Hybrid Polymer Composite Networks For Regulating Cellular Behavior, Charles Henley Sprague
Photo-crosslinkable polymeric biomaterials have emerged in the field of biomedical research to promote tissue regeneration. For example, scaffolds that can be crosslinked and hardened in situ have been known to make suitable implant alternatives. Since injectable and photo-crosslinkable biomaterials offer the advantage of being minimally invasive, they have emerged to compete with autografts, a current highly invasive method to repair diseased tissue. A series of novel photo-crosslinkable, injectable, and biodegradable nano-hybrid polymers consisting of poly(ε-caprolactone fumarate) (PCLF) and polyhedral oligomeric silsesquioxane (POSS) has been synthesized in our laboratory via polycondensation. To engineer the material properties of the nano-hybrid networks ...
Thimerosal Induces Apoptosis In A Neuroblastoma Model Via The Cjun N-Terminal Kinase Pathway, 2015 Marshall University
Thimerosal Induces Apoptosis In A Neuroblastoma Model Via The Cjun N-Terminal Kinase Pathway, Michelle Herdman, Aileen Marcello, Ying Huang, Richard Niles, Sanjit Dhar, Kinsley Kinningham
Richard M Niles
The cJun N-terminal kinase (JNK)-signaling pathway is activated in response to a variety of stimuli, including environmental insults, and has been implicated in neuronal apoptosis. In this study, we investigated the role that the JNK pathway plays in neurotoxicity caused by thimerosal, an ethylmercury-containing preservative. SK-N-SH cells treated with thimerosal (0-10 microM) showed an increase in the phosphorylated (active) form of JNK and cJun with 5 and 10 microM thimerosal treatment at 2 and 4 h. To examine activator protein-1 (AP-1) transcription, cells were transfected with a pGL2 vector containing four AP-1 consensus sequences and then treated with thimerosal ...
Advanced Problem Solving In The Biotherapeutics Industry: Parameters Influencing The Delivery Of A Novel Cell Therapy Product And Exploration Of A New Method For Determining Activity Of Clostridium Histolyticum Collagenase, A Wound Debridement Enzyme, 2015 University of North Texas Health Science Center at Fort Worth
Advanced Problem Solving In The Biotherapeutics Industry: Parameters Influencing The Delivery Of A Novel Cell Therapy Product And Exploration Of A New Method For Determining Activity Of Clostridium Histolyticum Collagenase, A Wound Debridement Enzyme, Melanie A. Harris B.S.
Theses and Dissertations
Biotechnology is a multi-faceted industry with many unique challenges that require knowledge in a broad range of topics. When working in the wound care field it is necessary to not only create a product in the laboratory, but also effectively bring it to the patient. This task requires many skilled people who can test it for efficacy, design and conduct clinical trials, confirm quality and consistency, design packaging, consider transportation issues and so on. The following investigation focuses on the testing of a cellular product and its accompanying device under various conditions as well as the exploration of a new ...