Medical Molecular Biology Commons™
Open Access. Powered by Scholars. Published by Universities.™
23 Institutions 164 Full-Text Articles 505 Authors 35,792 Downloads
Recent Articles in Medical Molecular Biology
Induction Of Neurotrophic And Differentiation Genes In Neural Stem Cells By Valproic Acid, Walaa Saeed Almutawaa 
McMaster University
Induction Of Neurotrophic And Differentiation Genes In Neural Stem Cells By Valproic Acid, Walaa Saeed Almutawaa
Open Access Dissertations and Theses
Valproic acid (2-propylpentanoicacid) has long been in use as an anticonvulsant and mood-stabilizer. Recently, VPA has been shown to inhibit the activity of histone deacetylases (HDACs), resulting in chromatin remodelling and changes in gene expression. Although the molecular mechanism for VPA action in the central nervous is not well understood, many signalling pathways have been suggested as targets for this HDAC inhibitor. For instance, VPA was found to induce differentiation in adult hippocampal neural progenitor cells via the β-catenin-Ras-ERK pathway. Also, VPA up regulated Bcl-2, a neurotrophic/neuroprotective protein, with association of extracellular signal-regulated kinase (ERK-1) and phosphatidylinositol 3- kinase ...
The Pluripotency Proposition: A Biological And Ethical Case For The Utilization Of Hipscs In Place Of Hescs, Drew Dickson 
Liberty University
The Pluripotency Proposition: A Biological And Ethical Case For The Utilization Of Hipscs In Place Of Hescs, Drew Dickson
Senior Honors Papers
Human embryonic stem cell (hESC) research has spurred ethical controversy ever since it became feasible in 1998. The reason for this is due to the fact that hESC research requires the destruction of a human embryo, thereby causing the cessation of life for that developing human. Despite this unavoidable consequence, many advocates of hESC research hold to the belief that the embryo is not actually a human person, and therefore deem the destruction of the embryo as justifiable. Many advocates of hESC research also have pointed to the unprecedented medical potential of hESCs to argue in favor of their case ...
Preventing Mature Biofilm Formation On Colonized Surfaces Using Antibiotics, Casey Shere' Seldon, Brandon Kapalko 
Georgia State University
Preventing Mature Biofilm Formation On Colonized Surfaces Using Antibiotics, Casey Shere' Seldon, Brandon Kapalko
Georgia State Undergraduate Research Conference
No abstract provided.
The Role Of Dax-1 In Regulating Pluripotency In Mouse Embryonic Stem Cells, Anthony Torres 
University of San Francisco
The Role Of Dax-1 In Regulating Pluripotency In Mouse Embryonic Stem Cells, Anthony Torres
Master Theses
The orphan receptor Dax-1 is highly expressed in pluripotent embryonic stem (ES) cells and shows a correlative reduction in expression as these cells differentiate. While it is known that Dax-1 is expressed in pluripotent mouse ES cells, the precise function of Dax-1 in these cells is not as well understood. Recent studies employing RNA interference (RNAi) to specifically reduce the expression of the Dax-1 gene in mouse ES cells found that upon the knock down of Dax-1, ES cells differentiated. These findings indicate that Dax-1 functions in a novel role in the maintenance of a relatively undifferentiated state in ES ...
Sex Differences In Cell Death And Steroid Hormone Receptors In Cortical Explants, Amanda L. Trout 
University of Kentucky
Sex Differences In Cell Death And Steroid Hormone Receptors In Cortical Explants, Amanda L. Trout
Theses and Dissertations--Physiology
Estrogens, such as the biologically active 17-b estradiol (E2) have many actions in the male and female brain. Not only does E2 regulate reproductive behavior in adults, it organizes and activates the brains of younger animals in a sex-specific manner. In addition, many human studies have shown E2 to provide protection against a variety of neurological disorders, including stoke. These studies have been controversial and depend largely on the type and timing of hormone replacement. Animal studies are much less controversial and clearly demonstrate a neuroprotective role for E2 following ischemic brain injury. Because much of E2 neuroprotection requires sex ...
Possible Steps Of Complete Disassembly Of Post-Termination Complex By Yeast Eef3 Deduced From Inhibition By Translocation Inhibitors., Shinya Kurata, Ben Shen, Jun O Liu, Nono Takeuchi, Akira Kaji, Hideko Kaji 
Thomas Jefferson University
Possible Steps Of Complete Disassembly Of Post-Termination Complex By Yeast Eef3 Deduced From Inhibition By Translocation Inhibitors., Shinya Kurata, Ben Shen, Jun O Liu, Nono Takeuchi, Akira Kaji, Hideko Kaji
Department of Biochemistry and Molecular Biology Faculty Papers
Ribosomes, after one round of translation, must be recycled so that the next round of translation can occur. Complete disassembly of post-termination ribosomal complex (PoTC) in yeast for the recycling consists of three reactions: release of tRNA, release of mRNA and splitting of ribosomes, catalyzed by eukaryotic elongation factor 3 (eEF3) and ATP. Here, we show that translocation inhibitors cycloheximide and lactimidomycin inhibited all three reactions. Cycloheximide is a non-competitive inhibitor of both eEF3 and ATP. The inhibition was observed regardless of the way PoTC was prepared with either release factors or puromycin. Paromomycin not only inhibited all three reactions ...
Libraries At The University Of Massachusetts Amherst: Seeking An International Perspective, Maxine G. Schmidt 
University of Massachusetts - Amherst
Libraries At The University Of Massachusetts Amherst: Seeking An International Perspective, Maxine G. Schmidt
Maxine G Schmidt
Presentation delivered to librarians in China, Japan and South Korea as part of my sabbatical research on the use of libraries by Asian students in their home countries.
Fusing The C-Terminal Tridecapeptide Of Hirudin To Α1-Proteinase Inhibitor M358r Accelerates Its Rate Of Thrombin Inhibition, Leigh Ann C. Roddick McMaster University
Fusing The C-Terminal Tridecapeptide Of Hirudin To Α1-Proteinase Inhibitor M358r Accelerates Its Rate Of Thrombin Inhibition, Leigh Ann C. Roddick
Open Access Dissertations and Theses
The serpin α-1 proteinase inhibitor (API) normally only impacts the coagulation cascade through its ability to inactivate factor XIa. However, the point mutation (Met to Arg) at position 358 results in a potent thrombin inhibitor, API M358R. This mutation also enhances this serpin’s ability to inhibit the anticoagulant protein, activated protein C (APC) and hence this property limits its therapeutic potential. As a result, various modifications to this protein have been engineered in order to enhance its specificity towards thrombin. Previously, the Heparin Cofactor II (HCII) N-terminal tail, HCII 1-75, which binds exosite 1 of thrombin, was tethered to ...
Development Of A Protocol To Measure Gene Expression In The Mouse Tibia, Daniel Hoover 
California Polytechnic State University
Development Of A Protocol To Measure Gene Expression In The Mouse Tibia, Daniel Hoover
Biomedical Eng/General Eng.
Numerous molecular factors active in bone tissue direct fracture repair and remodeling which can be altered by disease conditions such as Peripheral Arterial Disease (PAD) and Osteoporosis. Methods of molecular biology are commonly applied to investigate the expression and role of these molecular factors. This project presents a robust three-step protocol for examining gene expression in the mouse tibia. The protocol begins with isolating RNA from a flash frozen tibia sample. The isolated RNA is reverse transcribed into cDNA. Finally, PCR is performed to indentify expressed genes. Establishing this protocol will allow further research into the mechanisms of bone remodeling ...
Regulation Of Lipogenesis By Cyclin-Dependent Kinase 8-Mediated Control Of Srebp-1., Xiaoping Zhao, Daorong Feng, Qun Wang, Arian Abdulla, Xiao-Jun Xie, Jie Zhou, Yan Sun, Ellen S Yang, Lu-Ping Liu, Bhavapriya Vaitheesvaran, Lauren Bridges, Irwin J Kurland, Randy Strich, Jian-Quan Ni, Chenguang Wang, Johan Ericsson, Jeffrey E Pessin, Jun-Yuan Ji, Fajun Yang Thomas Jefferson University
Regulation Of Lipogenesis By Cyclin-Dependent Kinase 8-Mediated Control Of Srebp-1., Xiaoping Zhao, Daorong Feng, Qun Wang, Arian Abdulla, Xiao-Jun Xie, Jie Zhou, Yan Sun, Ellen S Yang, Lu-Ping Liu, Bhavapriya Vaitheesvaran, Lauren Bridges, Irwin J Kurland, Randy Strich, Jian-Quan Ni, Chenguang Wang, Johan Ericsson, Jeffrey E Pessin, Jun-Yuan Ji, Fajun Yang
Department of Cancer Biology Faculty Papers
Altered lipid metabolism underlies several major human diseases, including obesity and type 2 diabetes. However, lipid metabolism pathophysiology remains poorly understood at the molecular level. Insulin is the primary stimulator of hepatic lipogenesis through activation of the SREBP-1c transcription factor. Here we identified cyclin-dependent kinase 8 (CDK8) and its regulatory partner cyclin C (CycC) as negative regulators of the lipogenic pathway in Drosophila, mammalian hepatocytes, and mouse liver. The inhibitory effect of CDK8 and CycC on de novo lipogenesis was mediated through CDK8 phosphorylation of nuclear SREBP-1c at a conserved threonine residue. Phosphorylation by CDK8 enhanced SREBP-1c ubiquitination and protein ...
Engrailed Cooperates Directly With Extradenticle And Homothorax On A Distinct Class Of Homeodomain Binding Sites To Repress Sloppy Paired., Miki Fujioka, Brian Gebelein, Zenobia C Cofer, Richard S Mann, James B Jaynes Thomas Jefferson University
Engrailed Cooperates Directly With Extradenticle And Homothorax On A Distinct Class Of Homeodomain Binding Sites To Repress Sloppy Paired., Miki Fujioka, Brian Gebelein, Zenobia C Cofer, Richard S Mann, James B Jaynes
Department of Biochemistry and Molecular Biology Faculty Papers
Even skipped (Eve) and Engrailed (En) are homeodomain-containing transcriptional repressors with similar DNA binding specificities that are sequentially expressed in Drosophila embryos. The sloppy-paired (slp) locus is a target of repression by both Eve and En. At blastoderm, Eve is expressed in 7 stripes that restrict the posterior border of slp stripes, allowing engrailed (en) gene expression to be initiated in odd-numbered parasegments. En, in turn, prevents expansion of slp stripes after Eve is turned off. Prior studies showed that the two tandem slp transcription units are regulated by cis-regulatory modules (CRMs) with activities that overlap in space and time ...
Popular Institutions
Popular Authors
Jeffrey Morris
COBRA
Jason Bowman
University of Rhode Island
Maxine Schmidt
University of Massachusetts Medical School
Maria Cekanova
University of Tennessee, Knoxville
Katrina Salazar
Texas Medical Center Library
Hideko Kaji
Thomas Jefferson University
Jingwei Xie
Marshall University
Anthony Torres
University of San Francisco
Veera Baladandayuthapani
University of Wollongong
Jay Reddy
University of Nebraska - Lincoln
Based on downloads this month
Popular Articles
A Mathematical Model For Dengue Fever In A Virgin Environment, Jason Bowman
An Introduction To High-Throughput Bioinformatics Data, Jeffrey Morris
Taz As A Regulator Of Mesenchymal Transformation And Clinical Aggressiveness In Gliomas, Katrina Salazar
Electrospun Nanofibers For Neural Tissue Engineering, Jingwei Xie
The Role Of Dax-1 In Regulating Pluripotency In Mouse Embryonic Stem Cells, Anthony Torres
Integrative Bayesian Analysis Of High-Dimensional Multi-Platform Genomics Data, Jeffrey Morris, Veera Baladandayuthapani
Effects Of Extreme Temperature On Airway Smooth Muscle Cell Death, Lindsay DoHarris
Food Based Approaches For A Healthy Nutrition In Africa
Effect Of Estrogen On Pseudomonas Mucoidy And Exacerbations In Cystic Fibrosis, Borislav Dimitrov, Catherine Greene
Based on downloads this month