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Microbial Load Of Touch Screen Mobile Phones Used By University Students And Healthcare Staff, Abdelraouf Elmanama 2020 Islamic University-Gaza

Microbial Load Of Touch Screen Mobile Phones Used By University Students And Healthcare Staff, Abdelraouf Elmanama

Journal of the Arab American University مجلة الجامعة العربية الامريكية للبحوث

Mobile phones have become an indispensable part of our lives. Though they offer plenty of advantages, they are prolific breeding grounds for infectious pathogens in communities and hospitals. The present study seeks to identify the counts and types of bacteria contaminating touch screen mobile phones (TSMP) used by students of the Islamic University-Gaza (IUG) and healthcare workers (HCWs) at Al-Shifa Hospital. It also tries to investigate the antimicrobial resistance profiles. A cross-sectional study was conducted from October 2013 to April 2014. Two hundred and fifty swab samples were collected: 100 IUG female students, 100 IUG male students and 50 from ...


Evaluation Of The Lateral Flow Immunoassays And Electrochemiluminescent Technique For Detection Of Hepatitis B, Eidha Hameed 2020 Hadramout University, Yemen

Evaluation Of The Lateral Flow Immunoassays And Electrochemiluminescent Technique For Detection Of Hepatitis B, Eidha Hameed

Journal of the Arab American University مجلة الجامعة العربية الامريكية للبحوث

Infection of hepatitis B virus (HBV) is the most common type of viral diseases worldwide; Hepatitis B surface antigen (HBsAg) is the principal target for laboratory tests to diagnose the HBV infection. Therefore, rapid tests for its diagnosis are less costly, and easy to perform and develop recently.

This study aimed to evaluate the reliability of lateral flow immunoassays (LFA) and electrochemiluminescent (ECL) technique for the detection of HBsAg in Hadhramout/Yemen.

A total of 85 serum samples were tested for HBsAg by ECL technique (cobas e 411) and three rapid LFA cassettes (INTEC, ACRO and HEALGEN). Sensitivity and specificity ...


Neurotropic Lineage Iii Strains Of Listeria Monocytogenes Disseminate To The Brain Without Reaching High Titer In The Blood, Taylor E. Senay, Jessica L. Ferrell, Filip G. Garrett, Taylor M. Albrecht, Jooyoung Cho, Katie L. Alexander, Tanya Myers-Morales, Olivia F. Grothaus, Sarah E. F. D'Orazio 2020 University of Kentucky

Neurotropic Lineage Iii Strains Of Listeria Monocytogenes Disseminate To The Brain Without Reaching High Titer In The Blood, Taylor E. Senay, Jessica L. Ferrell, Filip G. Garrett, Taylor M. Albrecht, Jooyoung Cho, Katie L. Alexander, Tanya Myers-Morales, Olivia F. Grothaus, Sarah E. F. D'Orazio

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Listeria monocytogenes is thought to colonize the brain using one of three mechanisms: direct invasion of the blood-brain barrier, transportation across the barrier by infected monocytes, and axonal migration to the brain stem. The first two pathways seem to occur following unrestricted bacterial growth in the blood and thus have been linked to immunocompromise. In contrast, cell-to-cell spread within nerves is thought to be mediated by a particular subset of neurotropic L. monocytogenes strains. In this study, we used a mouse model of foodborne transmission to evaluate the neurotropism of several L. monocytogenes isolates. Two strains preferentially colonized the brain ...


The Development Of Bacterial Magnetic Resonance Imaging For Microbiota Analyses, Sarah C. Donnelly 2020 The University of Western Ontario

The Development Of Bacterial Magnetic Resonance Imaging For Microbiota Analyses, Sarah C. Donnelly

Electronic Thesis and Dissertation Repository

Current microbial analyses to assess either the commensal microbiota or microorganism infection and disease typically require ex vivo techniques that risk contamination and are not undertaken in real time. The possibilities for employing imaging techniques in the microbiology field is becoming more prominent as studies expand on the use of positron emission tomography, ultrasound and numerous microscopy techniques. However, magnetic resonance imaging (MRI), a non-invasive in vivo modality that can produce real-time results is falling behind. Here, we examined the feasibility of detecting bacteria using clinical field strength MRI. Commensal, probiotic and uropathogenic Escherichia coli were scanned by 3 Tesla ...


Staphylococcus Aureus Atp Synthase Promotes Biofilm Persistence By Influencing Innate Immunity, Megan E. Bosch, Blake P. Bertrand, Cortney E. Heim, Abdulelah A. Alqarzaee, Sujata S. Chaudhari, Amy L. Aldrich, Paul D. Fey, Vinai C. Thomas, Tammy Kielian 2020 University of Nebraska Medical Center

Staphylococcus Aureus Atp Synthase Promotes Biofilm Persistence By Influencing Innate Immunity, Megan E. Bosch, Blake P. Bertrand, Cortney E. Heim, Abdulelah A. Alqarzaee, Sujata S. Chaudhari, Amy L. Aldrich, Paul D. Fey, Vinai C. Thomas, Tammy Kielian

Journal Articles: Pathology and Microbiology

No abstract provided.


Which Sample Type Is Better For Xpert Mtb/Rif To Diagnose Adult And Pediatric Pulmonary Tuberculosis?, Mengyuan Lyu, Jian Zhou, Yuhui Cheng, Weelic Chong, Kang Wu, Teng Fang, Tianbo Fu, Binwu Ying 2020 Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China; West China Medical School/West China Hospital, Sichuan University, Sichuan Province 610041, Chengdu, China

Which Sample Type Is Better For Xpert Mtb/Rif To Diagnose Adult And Pediatric Pulmonary Tuberculosis?, Mengyuan Lyu, Jian Zhou, Yuhui Cheng, Weelic Chong, Kang Wu, Teng Fang, Tianbo Fu, Binwu Ying

Department of Medicine Faculty Papers

OBJECTIVE: This review aimed to identify proper respiratory-related sample types for adult and pediatric pulmonary tuberculosis (PTB), respectively, by comparing performance of Xpert MTB/RIF when using bronchoalveolar lavage (BAL), induced sputum (IS), expectorated sputum (ES), nasopharyngeal aspirates (NPAs), and gastric aspiration (GA) as sample.

METHODS: Articles were searched in Web of Science, PubMed, and Ovid from inception up to 29 June 2020. Pooled sensitivity and specificity were calculated, each with a 95% confidence interval (CI). Quality assessment and heterogeneity evaluation across included studies were performed.

RESULTS: A total of 50 articles were included. The respective sensitivity and specificity were ...


Cell Cycle Regulation In Macrophages And Susceptibility To Hiv-1, Isabella A. T. M. Ferreira, James Zachary Porterfield, Ravindra K. Gupta, Petra Mlcochova 2020 University of Cambridge, UK

Cell Cycle Regulation In Macrophages And Susceptibility To Hiv-1, Isabella A. T. M. Ferreira, James Zachary Porterfield, Ravindra K. Gupta, Petra Mlcochova

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Macrophages are the first line of defence against invading pathogens. They play a crucial role in immunity but also in regeneration and homeostasis. Their remarkable plasticity in their phenotypes and function provides them with the ability to quickly respond to environmental changes and infection. Recent work shows that macrophages undergo cell cycle transition from a G0/terminally differentiated state to a G1 state. This G0-to-G1 transition presents a window of opportunity for HIV-1 infection. Macrophages are an important target for HIV-1 but express high levels of the deoxynucleotide-triphosphate hydrolase SAMHD1, which restricts viral DNA synthesis by decreasing levels of dNTPs ...


Deacetylation Of Hsd17b10 By Sirt3 Regulates Cell Growth And Cell Resistance Under Oxidative And Starvation Stresses., Lu Liu, Shuaiyi Chen, Miao Yu, Chenxu Ge, Mengmeng Ren, Boya Liu, Xin Yang, Thomas W Christian, Ya-Ming Hou, Junhua Zou, Wei-Guo Zhu, Jianyuan Luo 2020 Department of Medical Genetics, Center for Medical Genetics, Peking University Health Science Center, Beijing, 100191, China

Deacetylation Of Hsd17b10 By Sirt3 Regulates Cell Growth And Cell Resistance Under Oxidative And Starvation Stresses., Lu Liu, Shuaiyi Chen, Miao Yu, Chenxu Ge, Mengmeng Ren, Boya Liu, Xin Yang, Thomas W Christian, Ya-Ming Hou, Junhua Zou, Wei-Guo Zhu, Jianyuan Luo

Department of Biochemistry and Molecular Biology Faculty Papers

17-beta-hydroxysteroid dehydrogenase 10 (HSD17B10) plays an important role in mitochondrial fatty acid metabolism and is also involved in mitochondrial tRNA maturation. HSD17B10 missense mutations cause HSD10 mitochondrial disease (HSD10MD). HSD17B10 with mutations identified from cases of HSD10MD show loss of function in dehydrogenase activity and mitochondrial tRNA maturation, resulting in mitochondrial dysfunction. It has also been implicated to play roles in the development of Alzheimer disease (AD) and tumorigenesis. Here, we found that HSD17B10 is a new substrate of NAD-dependent deacetylase Sirtuin 3 (SIRT3). HSD17B10 is acetylated at lysine residues K79, K99 and K105 by the acetyltransferase CBP, and the ...


Lyssavirus Vaccine With A Chimeric Glycoprotein Protects Across Phylogroups, Christine R Fisher, David E Lowe, Todd G Smith, Yong Yang, Christina L Hutson, Christoph Wirblich, Gino Cingolani, Matthias J. Schnell 2020 Thomas Jefferson University

Lyssavirus Vaccine With A Chimeric Glycoprotein Protects Across Phylogroups, Christine R Fisher, David E Lowe, Todd G Smith, Yong Yang, Christina L Hutson, Christoph Wirblich, Gino Cingolani, Matthias J. Schnell

Department of Biochemistry and Molecular Biology Faculty Papers

Rabies is nearly 100% lethal in the absence of treatment, killing an estimated 59,000 people annually. Vaccines and biologics are highly efficacious when administered properly. Sixteen rabies-related viruses (lyssaviruses) are similarly lethal, but some are divergent enough to evade protection from current vaccines and biologics, which are based only on the classical rabies virus (RABV). Here we present the development and characterization of LyssaVax, a vaccine featuring a structurally designed, functional chimeric glycoprotein (G) containing immunologically important domains from both RABV G and the highly divergent Mokola virus (MOKV) G. LyssaVax elicits high titers of antibodies specific to both ...


Are You Still On The Fence About Wearing A Mask?, Richard Davis 2020 Department of Microbiology, Providence Sacred Heart Medical Center

Are You Still On The Fence About Wearing A Mask?, Richard Davis

Articles, Abstracts, and Reports

Microbe-containing droplets are produced by coughing, talking, singing and sneezing. Masks are effective at blocking most of these droplets, even when up close.


Convalescent Plasma In Covid-19, Ranjit S. Banwait, Joshua K. Salabei, Troy J. Fishman, Uma G. Iyer 2020 UCF/HCA

Convalescent Plasma In Covid-19, Ranjit S. Banwait, Joshua K. Salabei, Troy J. Fishman, Uma G. Iyer

HCA Healthcare Journal of Medicine

The recent pandemic of SARS-CoV-2, which causes novel coronavirus disease 2019 (Covid-19), has had devastating impact on a global and national scale. In order to overcome this outbreak it is imperative we find treatments that are safe and effective. To date, no definitive treatment is available that can curtail the spread of this viral syndrome. Convalescent plasma (CP) is one such option that has repeatedly served as an important tool in treatment of various bacterial and viral infections, especially in the setting of no specific antimicrobial or vaccination against an infectious disease. Herein, we review the history of CP, prior ...


Characterizing The Role Of The Microbiome In Kidney Stone Disease, Kaitlin F. Al 2020 The University of Western Ontario

Characterizing The Role Of The Microbiome In Kidney Stone Disease, Kaitlin F. Al

Electronic Thesis and Dissertation Repository

The goal of this thesis was to increase understanding of the role of the human microbiome in kidney stone disease, from stone nidus formation through to surgical stone treatment, using a combination of in vitro, in vivo, and human clinical investigations.

We first optimized our methods of sample collection for the use of such protocols in clinical studies involving urinary and gut microbiota investigations. We developed a novel method of fecal sampling that is amenable to study participants, inexpensive, and results in reliable downstream sequencing results.

We then utilized this sampling methodology in clinical investigations into the microbiota of surgical ...


Similac Special Care And Elecare Cause Neonatal Gut Injury In Mice, Karishma Rao, Heather L. Menden, Wei Yu, Inamul Haque, Susana Chavez-Bueno, Alain C. Cuna, Shahid Umar, Venkatesh Sampath 2020 CMH

Similac Special Care And Elecare Cause Neonatal Gut Injury In Mice, Karishma Rao, Heather L. Menden, Wei Yu, Inamul Haque, Susana Chavez-Bueno, Alain C. Cuna, Shahid Umar, Venkatesh Sampath

Research Days

No abstract provided.


Identifying Determinants Of Target Specificity In Two Related Bacterial Peptide Toxins, Andrew D. Holmes 2020 University of South Dakota

Identifying Determinants Of Target Specificity In Two Related Bacterial Peptide Toxins, Andrew D. Holmes

Honors Thesis

Toxin-antitoxin (TA) systems were originally identified as two-component systems ensuring the stable inheritance of plasmids in bacterial populations. Recently, they have been identified on bacterial chromosomes where their functions remain mostly undefined. The par locus of E. faecalis plasmid pAD1 (parpAD1) was the first TA system defined in a Gram-positive bacterium and a homolog encoded on the E. faecalis chromosome (parEF0409) was later described. Related loci numbering in the hundreds have been identified throughout Gram-positive bacteria based on homology to the toxin of the system, Fst, and similarities in genetic organization and regulation. Despite their similar sequences, over-expression ...


How Can We Stop Cancer?, Joseph R. Current 2020 St. John Fisher College

How Can We Stop Cancer?, Joseph R. Current

The Review: A Journal of Undergraduate Student Research

Cancer is a disease that humans have been struggling to combat for centuries. It originates from the accumulation of several mutations over the life of a cell that causes it to evade cell death and multiply rapidly. It can affect any tissue in the body and can spread to other parts of the body through metastasis. Cancer comes in numerous shapes and sizes with different levels of aggression, growth speeds, and health risks. Many treatments for cancer exist today, three of the most popular being surgery, chemotherapy, and radiation therapy, which can be used in combinations with other treatments to ...


How Immune T-Cell Augmentation Can Help Prevent Covid-19: A Possible Nutritional Solution Using Ketogenic Lifestyle, Ravi k. Kamepalli MD,FIDSA,CWSP 2020 Regional Infectious diseases and infusion ctr,Inc

How Immune T-Cell Augmentation Can Help Prevent Covid-19: A Possible Nutritional Solution Using Ketogenic Lifestyle, Ravi K. Kamepalli Md,Fidsa,Cwsp

The University of Louisville Journal of Respiratory Infections

No abstract provided.


Staphylococcus Aureus May Be Living In Your Nasal Cavity Right Now, Hannah Pedersen 2020 Concordia University St. Paul

Staphylococcus Aureus May Be Living In Your Nasal Cavity Right Now, Hannah Pedersen

Research and Scholarship Symposium Posters

Staphylococcus aureus is a dual role bacterium and is able to live commensally in some patients, but can cause disease in others. Methicillin resistant S. aureus (MRSA) causes life threatening disease in patients and can be very hard to treat since it is resistant to many antibiotics. In order for S. aureus to wreak havoc on the body, it must be able to have specific genes expressed to secrete toxins. These toxins are what causes the patients to get a wide variety of symptoms like boils, scalded skin, toxic shock syndrome, pneumonia or sepsis. This study tested eleven different isolates ...


Biological Sex Influences Susceptibility To Acinetobacter Baumannii Pneumonia In Mice, Sílvia Pires, Adeline Peignier, Jeremy Seto, Davida S. Smyth, Dane Parker 2020 Rutgers University - Newark

Biological Sex Influences Susceptibility To Acinetobacter Baumannii Pneumonia In Mice, Sílvia Pires, Adeline Peignier, Jeremy Seto, Davida S. Smyth, Dane Parker

Publications and Research

Acinetobacter baumannii (A. baumannii) is an extremely versatile multidrug-resistant pathogen with a very high mortality rate; therefore, it has become crucial to understand the host response during its infection. Given the importance of mice for modeling infection and their role in preclinical drug development, equal emphasis should be placed on the use of both sexes. Through our studies using a murine model of acute pneumonia with A. baumannii, we observed that female mice were more susceptible to infection. Likewise, treatment of male mice with estradiol increased their susceptibility to infection. Analysis of the airway compartment revealed enhanced inflammation and reduced ...


Approaches To Overcome Flow Cytometry Limitations In The Analysis Of Cells From Veterinary Relevant Species, Julia Hunka, John T. Riley, Gudrun F. Debes 2020 Thomas Jefferson University

Approaches To Overcome Flow Cytometry Limitations In The Analysis Of Cells From Veterinary Relevant Species, Julia Hunka, John T. Riley, Gudrun F. Debes

Department of Microbiology and Immunology Faculty Papers

BACKGROUND: Flow cytometry is a powerful tool for the multiparameter analysis of leukocyte subsets on the single cell level. Recent advances have greatly increased the number of fluorochrome-labeled antibodies in flow cytometry. In particular, an increase in available fluorochromes with distinct excitation and emission spectra combined with novel multicolor flow cytometers with several lasers have enhanced the generation of multidimensional expression data for leukocytes and other cell types. However, these advances have mainly benefited the analysis of human or mouse cell samples given the lack of reagents for most animal species. The flow cytometric analysis of important veterinary, agricultural, wildlife ...


The Impact Of Changes In Clinical Microbiology Laboratory Location And Ownership On The Practice Of Infectious Diseases, Michael Pentella, Melvin P. Weinstein, Susan E. Beekmann, Philip M. Polgreen, Richard T. Ellison III 2020 University of Iowa

The Impact Of Changes In Clinical Microbiology Laboratory Location And Ownership On The Practice Of Infectious Diseases, Michael Pentella, Melvin P. Weinstein, Susan E. Beekmann, Philip M. Polgreen, Richard T. Ellison Iii

Infectious Diseases and Immunology Publications

The number of onsite clinical microbiology laboratories in hospitals is decreasing, likely related to the business model for laboratory consolidation and labor shortages, and this impacts a variety of clinical practices including banking isolates for clinical or epidemiologic purposes. To determine the impact of these trends, infectious disease (ID) physicians were surveyed regarding their perceptions of offsite services. Clinical microbiology practices for retention of clinical isolates for future use were also determined. Surveys were sent to members of the Infectious Diseases Society of America's (IDSA) Emerging Infections Network (EIN). The EIN is a sentinel network of ID physicians who ...


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