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Cours Enzymologie Fondamentale Et Appliquée (Niveau Master 1), Mamoudou H. DICKO, Prof. 2016

Cours Enzymologie Fondamentale Et Appliquée (Niveau Master 1), Mamoudou H. Dicko, Prof.

Pr. Mamoudou H. DICKO, PhD

L'objectif principal de ce cours est de rappeler et donner aux étudiants les connaissances indispensables en enzymologie appliquée, à savoir :
- connaître la structure des enzymes, les méthodes d’extraction, de purification, de dosage et de caractérisation (électrophorèse, zymographie, etc.) des enzymes ;
- comprendre les principes fondamentaux de la cinétique chimique et la cinétique enzymatique y compris le rôle des inhibiteurs et autres effecteurs;
- comprendre les méthodes d’immobilisation des enzymes et leurs conséquences positives ou négatives dans la biocatalyse enzymatique;
- comprendre les différends domaines d’utilisation des enzymes en biochimie analytique, bio-industries, industrie agro-alimentaires, analyses bio-médicales, en diagnostic, etc.


Td Enzymo L3s5_2016.Pdf, Mamoudou H. DICKO, Prof. 2016

Td Enzymo L3s5_2016.Pdf, Mamoudou H. Dicko, Prof.

Pr. Mamoudou H. DICKO, PhD

Travaux Dirigés (TD) d'Enzymologie


Consolidating And Exploring Antibiotic Resistance Gene Data Resources, Mr Basil Britto Xavier, Anupam J Das, Guy Cochrane, Sandra De Ganck, Samir Kumar-Singh, Frank Moller Aarestrup, Herman Goossens, Surbhi Malhotra-Kumar 2016 University of Antwerp

Consolidating And Exploring Antibiotic Resistance Gene Data Resources, Mr Basil Britto Xavier, Anupam J Das, Guy Cochrane, Sandra De Ganck, Samir Kumar-Singh, Frank Moller Aarestrup, Herman Goossens, Surbhi Malhotra-Kumar

Anupam Das

The unrestricted use of antibiotics has resulted in rapid acquisition of antibiotic resistance (AR) and spread of multi-drug-resistant (MDR) bacterial pathogens. With the advent of next generation sequencing technologies and their application in understanding MDR pathogen dynamics, it has become imperative to unify AR gene data resources for easy accessibility for researchers. However, due to the absence of a centralized platform for AR gene resources, availability, consistency and accuracy of information vary considerably across different databases. In this article, we sought to explore existing AR gene data resources in order to make them more visible to the clinical microbiology community ...


Vitamin D Deficiency: "At Risk" Patient Populations And Potential Drug Interactions, Dana Lycans MD, Elias Salloum MD, Matthew K. Wingate MD, Thomas Melvin MD, Grant S. Buchanan MD, Franklin D. Shuler MD, PhD 2016 Marshall University

Vitamin D Deficiency: "At Risk" Patient Populations And Potential Drug Interactions, Dana Lycans Md, Elias Salloum Md, Matthew K. Wingate Md, Thomas Melvin Md, Grant S. Buchanan Md, Franklin D. Shuler Md, Phd

Marshall Journal of Medicine

Vitamin D is known to play an essential role in calcium homeostasis; however, excessive amounts can have harmful effects. Calcium and vitamin D levels are known to be influenced by drug interactions and pathology ranging from of cancer to cardiovascular disease. Vitamin D supplementation has become widespread, and it is important for clinicians to understand the way that certain conditions and medications interact with vitamin D and calcium homeostasis. The purpose of this review is to outline the benefits and adverse effects of vitamin D and how its levels are affected by certain pathologic and pharmacologic interactions.


Why Does Obesity Lead To Hypertension? Further Lessons From The Intersalt Study., Preeya T. Shah, Anna P. Shapiro, Zeid Khitan MD, Prasanna Santhanam MD, Joseph I. Shapiro MD 2016 Joan C Edwards School of Medicine

Why Does Obesity Lead To Hypertension? Further Lessons From The Intersalt Study., Preeya T. Shah, Anna P. Shapiro, Zeid Khitan Md, Prasanna Santhanam Md, Joseph I. Shapiro Md

Marshall Journal of Medicine

Objectives

To analyze correlations between major determinants of blood pressure (BP), in efforts to generate and compare predictive models that explain for variance in systolic, diastolic, and mean BP amongst participants of the Intersalt study.

Methods

Data from the Intersalt study, consisting of nearly 10,000 subjects from 32 different countries, were reviewed and analyzed. Published mean values of 24 hour urinary electrolyte excretion (Na+, K+), 24 hour urine creatinine excretion, body mass index (BMI, kg/m^2), and blood pressure data were extracted and imported into Matlab™ for stepwise linear regression analysis.

Results

As shown earlier, strong correlations between ...


Going Mainstream Or Just A Passing Fad? The Future Of The Ancestral Health Movement, Hamilton M. Stapell 2016 SUNY New Paltz

Going Mainstream Or Just A Passing Fad? The Future Of The Ancestral Health Movement, Hamilton M. Stapell

Journal of Evolution and Health

The current ancestral health (“paleo”) movement is often thought to be on the verge of going mainstream. Many within the movement believe this would lead to positive health and financial outcomes for both individuals and society as a whole. However, the transition from a small, highly-devoted group of adherents to a mass following will be far more difficult than commonly assumed. This paper argues there are three main obstacles to it becoming a mass phenomenon in the United States. First, Neolithic foods are tightly woven into the fabric of our culture (for example, bread within the Christian tradition). Second, refined ...


A New Genotype Of Bovine Leukemia Virus In South America Identified By Ngs-Based Whole Genome Sequencing And Molecular Evolutionary Genetic Analysis., Meripet Polat, Shin-Nosuke Takeshima, Kazuyoshi Hosomichi, Jiyun Kim, Taku Miyasaka, Kazunori Yamada, Mariluz Araínga, Tomoyuki Murakami, Yuki Matsumoto, Veronica de la Barra Diaz, Carlos Javier Panei, Ester Teresa González, Misao Kanemaki, Misao Onuma, Guillermo Giovambattista, Yoko Aida 2016 RIKEN, Japan

A New Genotype Of Bovine Leukemia Virus In South America Identified By Ngs-Based Whole Genome Sequencing And Molecular Evolutionary Genetic Analysis., Meripet Polat, Shin-Nosuke Takeshima, Kazuyoshi Hosomichi, Jiyun Kim, Taku Miyasaka, Kazunori Yamada, Mariluz Araínga, Tomoyuki Murakami, Yuki Matsumoto, Veronica De La Barra Diaz, Carlos Javier Panei, Ester Teresa González, Misao Kanemaki, Misao Onuma, Guillermo Giovambattista, Yoko Aida

Journal Articles: Pharmacology & Experimental Neuroscience

BACKGROUND: Bovine leukemia virus (BLV) is a member of retroviridae family, together with human T cell leukemia virus types 1 and 2 (HTLV-1 and -2) belonging to the genes deltaretrovirus, and infects cattle worldwide. Previous studies have classified the env sequences of BLV provirus from different geographic locations into eight genetic groups. To investigate the genetic variability of BLV in South America, we performed phylogenetic analyses of whole genome and partial env gp51 sequences of BLV strains isolated from Peru, Paraguay and Bolivia, for which no the molecular characteristics of BLV have previously been published, and discovered a novel BLV ...


Colistin-Resistant Escherichia Coli Harbouring Mcr-1 Isolated From Food Animals In Hanoi, Vietname, Surbhi Malhotra-Kumar, Mr Basil Britto Xavier, Anupam J Das, Christine Lammens, Ha Thi Thu Hoang, Ngoc Thi Pham, Herman Goossens 2016 University of Antwerp

Colistin-Resistant Escherichia Coli Harbouring Mcr-1 Isolated From Food Animals In Hanoi, Vietname, Surbhi Malhotra-Kumar, Mr Basil Britto Xavier, Anupam J Das, Christine Lammens, Ha Thi Thu Hoang, Ngoc Thi Pham, Herman Goossens

Anupam Das

In The Lancet Infectious Diseases, Yi-Yun Liu and colleagues 1 reported, for the first time, plasmid-mediated colistin resistance in Escherichia coli isolated from animals, food, and patients in China. We screened 24 extended-spectrum β-lactamase (ESBL, blaCTX-M) harbouring E coli isolated during 2014–15 from rectal swabs taken from chickens on two farms (n=11) in the Van Lam district of the Hung Yen province, and from a pig farm (seven) and a pig slaughterhouse (four) located in the Hoai Duc region of the Hanoi province, Vietnam. All strains were screened for the presence of mcr-1 using PCR and Sanger sequencing ...


In-Silico Identification Of Prognostically Inversely Correlated Mirnas And Mrnas (Pic’S) In Multiple Cancers, Chirayu Pankaj Goswami, Zi-Xuan Wang 2016 Thomas Jefferson University

In-Silico Identification Of Prognostically Inversely Correlated Mirnas And Mrnas (Pic’S) In Multiple Cancers, Chirayu Pankaj Goswami, Zi-Xuan Wang

Pathology, Anatomy and Cell Biology Posters

Despite numerous methods available to identify potential mRNA targets for miRNAs, prognostic relationship of these molecules in diseases like cancers where deregulation of gene expression is a major pathogenic factor, has not yet been emphasized. We performed in-silico identification of prognostically inversely correlated miRNA - mRNA pairs (PIC’s) in multiple cancers using expression data from The Cancer Genome Atlas. Partners in a PIC show inverse correlation of expression and opposite hazard implication. Using a three step approach, we identified a total of 1,253,443 PIC’s from 23 cancer types, several of which have previously been shown to have ...


Increased Concentration Of Iodide In Airway Secretions Is Associated With Reduced Rsv Disease Severity, Rachel Derscheid, Albert van Geelen, Abigail Berkebile, Jack Gallup, Shannon Hostetter, Botond Banfi, Paul McCray, Mark Ackermann 2016 Iowa State University

Increased Concentration Of Iodide In Airway Secretions Is Associated With Reduced Rsv Disease Severity, Rachel Derscheid, Albert Van Geelen, Abigail Berkebile, Jack Gallup, Shannon Hostetter, Botond Banfi, Paul Mccray, Mark Ackermann

Jack M Gallup

Recent studies have revealed that the human and non-rodent mammalian airway mucosa contains an oxidative host defense system. This three-component system consists of hydrogen peroxide (H2O2)-producing enzymes dual oxidase (Duox) 1 and 2, thiocyanate (SCN-), and secreted lactoperoxidase (LPO). The LPO-catalyzed reaction between H2O2 and SCN- yields the bactericidal hypothiocyanite (OSCN-) in airway surface liquid (ASL). Although SCN- is the physiological substrate of LPO, the Duox/LPO/halide system can also generate hypoiodous acid (HOI) when the iodide (I-) concentration is elevated in ASL. Since HOI, but not OSCN-, inactivates respiratory syncytial virus (RSV) in cell culture, we used ...


Effects Of Formalin-Inactivated Respiratory Syncytial Virus (Fi-Rsv) In The Perinatal Lamb Model Of Rsv, Rachel Derscheid, Jack Gallup, Cory Knudson, Steven Varga, Drew Grosz, Albert Van Geelen, Shannon Hostetter, Mark Ackermann 2016 Iowa State University

Effects Of Formalin-Inactivated Respiratory Syncytial Virus (Fi-Rsv) In The Perinatal Lamb Model Of Rsv, Rachel Derscheid, Jack Gallup, Cory Knudson, Steven Varga, Drew Grosz, Albert Van Geelen, Shannon Hostetter, Mark Ackermann

Jack M Gallup

Respiratory syncytial virus (RSV) is the most frequent cause of bronchiolitis in infants and children worldwide. There are currently no licensed vaccines or effective antivirals. The lack of a vaccine is partly due to increased caution following the aftermath of a failed clinical trial of a formalin-inactivated RSV vaccine (FI-RSV) conducted in the 1960’s that led to enhanced disease, necessitating hospitalization of 80% of vaccine recipients and resulting in two fatalities. Perinatal lamb lungs are similar in size, structure and physiology to those of human infants and are susceptible to human strains of RSV that induce similar lesions as ...


Mast Cell Density And Substance P-Like Immunoreactivity During The Initiation And Progression Of Lung Lesions In Ovinemannheimia (Pasteurella) Haemolytica Pneumonia, Rafael Ramírez-Romero, Kim Brogden, Jack Gallup, Ioana Sonea, Mark Ackermann 2016 Iowa State University

Mast Cell Density And Substance P-Like Immunoreactivity During The Initiation And Progression Of Lung Lesions In Ovinemannheimia (Pasteurella) Haemolytica Pneumonia, Rafael Ramírez-Romero, Kim Brogden, Jack Gallup, Ioana Sonea, Mark Ackermann

Jack M Gallup

To determine the density of mast cells (MCs) and the extent of substance P (SP) immunoreactivity during initiation and progression of pneumonic pasteurellosis (PP), 18 lambs were inoculated intrabronchially withMannheimia (Pasteurella) haemolytica or saline, and lung tissue was collected at 1, 15 and 45 days post-inoculation (n=3, each group). Additionally, the left (non-inoculated) contralateral lungs in bacteria-inoculated animals were collected as controls. At 1 day after bacterial inoculation the lungs had typical M. haemolyticalesions. These pneumonic lesions had fewer numbers of MCs and reduced histamine content. Macrophages infiltrating some of the inflamed areas were strongly immunoreactive for ...


Low-Dose Aronia Melanocarpa Concentrate Attenuates Paraquat-Induced Neurotoxicity, Adam J. Case, Daniel Agraz, Iman M. Ahmad, Matthew C. Zimmerman 2016 University of Nebraska Medical Center

Low-Dose Aronia Melanocarpa Concentrate Attenuates Paraquat-Induced Neurotoxicity, Adam J. Case, Daniel Agraz, Iman M. Ahmad, Matthew C. Zimmerman

Journal Articles: Cellular & Integrative Physiology

No abstract provided.


Structure Of The Polyisoprenyl-Phosphate Glycosyltransferase Gtrb And Insights Into The Mechanism Of Catalysis, Chiara Ardiccioni, Oliver B. Clarke, David Tomasek, Habon A. Issa, Desiree C. von Alpen, Heather L. Pond, M. Chiara Manzini, +11 additional authors 2016 George Washington University

Structure Of The Polyisoprenyl-Phosphate Glycosyltransferase Gtrb And Insights Into The Mechanism Of Catalysis, Chiara Ardiccioni, Oliver B. Clarke, David Tomasek, Habon A. Issa, Desiree C. Von Alpen, Heather L. Pond, M. Chiara Manzini, +11 Additional Authors

Pharmacology and Physiology Faculty Publications

The attachment of a sugar to a hydrophobic polyisoprenyl carrier is the first step for all extracellular glycosylation processes. The enzymes that perform these reactions, polyisoprenyl-glycosyltransferases (PI-GTs) include dolichol phosphate mannose synthase (DPMS), which generates the mannose donor for glycosylation in the endoplasmic reticulum. Here we report the 3.0Å resolution crystal structure of GtrB, a glucose-specific PI-GT from Synechocystis, showing a tetramer in which each protomer contributes two helices to a membrane-spanning bundle. The active site is 15 Å from the membrane, raising the question of how water-soluble and membrane-embedded substrates are brought into apposition for catalysis. A conserved ...


Autoimmunity In Picornavirus Infections, Chandirasegara Massilamany, Andreas Koenig, Jay Reddy, Sally Huber, Iwona Buskiewicz 2016 University of Nebraska-Lincoln

Autoimmunity In Picornavirus Infections, Chandirasegara Massilamany, Andreas Koenig, Jay Reddy, Sally Huber, Iwona Buskiewicz

Papers in Veterinary and Biomedical Science

Enteroviruses are small, non-enveloped, positive-sense singlestrand RNA viruses, and are ubiquitously found throughout the world. These viruses usually cause asymptomatic or mild febrile illnesses, but have a propensity to induce severe diseases including type 1 diabetes and pancreatitis, paralysis and neuroinflammatory disease, myocarditis, or hepatitis. This pathogenicity may result from induction of autoimmunity to organ-specific antigens. While enterovirus-triggered autoimmunity can arise from multiple mechanisms including antigenic mimicry and release of sequestered antigens, the recent demonstration of T cells expressing dual T cell receptors arising as a natural consequence of Theiler’s virus infection is the first demonstration of this autoimmune ...


A Urinary Metabolic Signature For Multiple Sclerosis And Neuromyelitis Optica, Teklab Gebregiworgis, Helle H. Nielsen, Chandirasegara Massilamany, Arunakumar Gangaplara, Jay Reddy, Zsolt Illes, Robert Powers 2016 University of Nebraska–Lincoln

A Urinary Metabolic Signature For Multiple Sclerosis And Neuromyelitis Optica, Teklab Gebregiworgis, Helle H. Nielsen, Chandirasegara Massilamany, Arunakumar Gangaplara, Jay Reddy, Zsolt Illes, Robert Powers

Jay Reddy Publications

Urine is a metabolite-rich biofluid that reflects the body’s effort to maintain chemical and osmotic homeostasis. Clinical diagnosis routinely relies on urine samples because the collection process is easy and noninvasive. Despite these advantages, urine is an under-investigated source of biomarkers for multiple sclerosis (MS). Nuclear magnetic resonance spectroscopy (NMR) has become a common approach for analyzing urinary metabolites for disease diagnosis and biomarker discovery. For illustration of the potential of urinary metabolites for diagnosing and treating MS patients, and for differentiating between MS and other illnesses, 38 urine samples were collected from healthy controls, MS patients, and neuromyelitis ...


The Current Approach To Diagnosis And Management Of Left Ventricular Noncompaction Cardiomyopathy: Review Of The Literature, Ronald Freudenberger MD, Courtney E. Bennett 2016 Lehigh Valley Health Network

The Current Approach To Diagnosis And Management Of Left Ventricular Noncompaction Cardiomyopathy: Review Of The Literature, Ronald Freudenberger Md, Courtney E. Bennett

Department of Medicine

No abstract provided.


Nurse Practitioners' Discussion Of Sexual Identity, Attraction And Behavior, Sarah J. McLaughlin 2016 University of Vermont

Nurse Practitioners' Discussion Of Sexual Identity, Attraction And Behavior, Sarah J. Mclaughlin

Graduate College Dissertations and Theses

ABSTRACT

Background: Sexual orientation is comprised of distinct components, including sexual identity, sexual attraction and sexual behavior. Lesbian, gay and bisexual adolescents are at an increased risk of experiencing poor health outcomes compared to non-sexual minority youth. Health care professional organizations recommend that health care providers discuss each component of sexual orientation at every adolescent health supervision visits in order to best assess the adolescent's health risks and needs for intervention and education.

Objective: This survey assessed the frequency with which nurse practitioners (NPs) in the state of Vermont discussed sexual identity, attraction and behavior with adolescents during annual ...


Incidence And Factors Associated With Nonalcoholic Fatty Liver Disease Among Patients With Rheumatoid Arthritis, Ani K. John 2016 Walden University

Incidence And Factors Associated With Nonalcoholic Fatty Liver Disease Among Patients With Rheumatoid Arthritis, Ani K. John

Walden Dissertations and Doctoral Studies

Nonalcoholic fatty liver disease (NAFLD) has become one of the most common hepatic diseases worldwide, making the diagnosis and management of NAFLD an emerging public health issue. Theories associated with NAFLD surmise that inflammation may be the root cause, along with the complex interplay of other chronic conditions such as obesity, metabolic syndrome, diabetes, dyslipidemia, and cardiovascular disease (CVD). It is unknown if other inflammatory conditions such as rheumatoid arthritis (RA), along with the use of methotrexate (MTX), might confer increased risk for NAFLD. Longitudinal data collected from a retrospective cohort of 17,481 adult RA patients in the United ...


Mechanisms Of Action Of Antiseizure Drugs And The Ketogenic Diet., Michael A. Rogawski, Jong M. Rho, Wolfgang Löscher 2015

Mechanisms Of Action Of Antiseizure Drugs And The Ketogenic Diet., Michael A. Rogawski, Jong M. Rho, Wolfgang Löscher

Michael A. Rogawski

Antiseizure drugs (ASDs), also termed antiepileptic drugs, are the main form of symptomatic treatment for people with epilepsy, but not all patients become free of seizures. The ketogenic diet is one treatment option for drug-resistant patients. Both types of therapy exert their clinical effects through interactions with one or more of a diverse set of molecular targets in the brain. ASDs act by modulation of voltage-gated ion channels, including sodium, calcium, and potassium channels; by enhancement of gamma-aminobutyric acid (GABA)-mediated inhibition through effects on GABA-A receptors, the GABA transporter 1 (GAT1) GABA uptake transporter, or GABA transaminase; through interactions ...


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