Engineering Commons^™

Strategies To Improve Productivity Of Cho-S Cells Expressing An Anti-Tnfα Monoclonal Antibody With Biosimilar Potential, Ana M. Moro, Matheus Luchese, Lilian Tsuruta, Wagner Quintilio, Mariana Lopes Dos Santos

Cell Culture Engineering XVI

Tumor necrosis factor alpha (TNFα) is a proinflammatory cytokine that mediates the homeostasis of immune responses; its exacerbated production is associated with the pathogenesis of autoimmune and chronic inflammatory diseases. Anti-TNFα drugs have revolutionized the treatment of inflammatory conditions such as rheumatoid arthritis and Crohn’s disease. Currently, a worldwide race is on stage for the production of biosimilars moved by patent expiration of monoclonal antibodies (mAbs), such as anti-TNFα adalimumab. This project is based on the first development stage for an adalimumab biosimilar candidate with potential for national production through the generation of a productive and stable cell line and …

Off-Target At-Scale Scale Down Model Verification Of A Marketed Biopharmaceutical, Markus Emmler, Stefanie Menrad, Yosuke Watanabe

Cell Culture Engineering XVI

According to authority guidelines, process understanding is considered the key factor for a successful control strategy in submissions for biopharmaceutical production processes. This knowledge is usually gained in scale down model (SDM) experiments and therefore is subject to the ability of the SDM to reliably predict behavior of cell culture processes in manufacturing scale. In order to prove predictability, SDM runs at target conditions are typically compared to manufacturing scale target runs only. At the same time, predictability is also assumed within the whole design space described or even beyond as evaluated in process design studies. The proof at off-target …

Development Of A Platform Expression System Using Targeted Integration In Chinese Hamster Ovary Cells, Scott Bahr, Trissa Borgschulte, Michael Johns, Jeff Galligan

Cell Culture Engineering XVI

In recent years the biomanufacturing industry has seen significant improvements in recombinant protein production titers due to advancements in protein expression technologies as well as media, feed and manufacturing process development. However, the standard methods of recombinant cell line development have remained relatively unchanged. The majority of the biopharma industry introduces transgenes into Chinese Hamster Ovary (CHO) cells using mechanical or chemical transfection processes followed by metabolic or antibiotic selection of stable recombinant pools. Through this process, the transgene(s) are randomly integrated into the genome, often times resulting in significant heterogeneity within the stable pools. Individual recombinant CHO cells within …

Curation Of A Cho Dg44 Genome Scale Model And Application To Support Cell Culture Development Process, Cyrielle Calmels, Laetitia Malphettes, Mikael Rørdam Andersen

Cell Culture Engineering XVI

Genome scale models (GSM) have become a useful tool to connect different omics dataset into a single computational framework, thus giving a good overview of the flux distribution and metabolites interconnections in a specific environmental condition. A community genome-scale metabolic network reconstruction of Cricetulus griseus and cell line specific models have been recently developed 1. The main objectives with the use of the published CHO DG44 model were to enhance industrial bioprocess performance by suggesting genetic or metabolic targets, as well as strategies for medium optimization, and by bringing more fundamental knowledge about CHO cell metabolism. In a first step, …

The Relevance Of Cell Size In A Cho Fed Batch Process: Metabolic And Transcriptomic Characterization, Dirk Martens, Xiao Pan, Rene Wijffels, Ciska Dalm

Cell Culture Engineering XVI

Normally the growth profile of a CHO cell fed-batch process can be divided into a growth phase followed by a stationary (non-growth) phase and a cell death phase. In this study, using a commercially available media system in a 10 liter fed-batch reactor, the growth phase is followed by a phase where cell division stops but cell growth continues in the form of an increase in cell size (Figure 1). During the cell size increase (SI) phase, the average volume and dry weight per cell increase linearly with time up to nearly threefold. Cell cycle and transcriptome analysis show that …

Tools And Methods For Providing Assurance Of Clonality For Legacy Cell Lines, Paul Wu, Juhi Ohja, John Thrift, Taymar Hartman, Louise Almond, Jennitte Stevens, Sam Yaghmour, Christina Alves, David Shaw, Mike Laird, Robyn Emmins, Yuan Zhu, Ren Liu, Zhimei Du, Rolf Koehler, Thomas Jostock, Karin Anderson, Howard Clarke, Chris Campbell

Cell Culture Engineering XVI

Over the last several years demonstration of cell line clonality has been a topic of many industry and regulatory presentations and papers. Guidance has been provided by the regulatory authorities, especially the FDA, on a path forward for providing evidence of clonality with high probability. It has been recommended that two-rounds of limiting dilution cloning (LDC) at sufficiently low seeding densities (≤0.5 cells/well) provides sufficient evidence that a cell line is clonal. Furthermore, one-round of LDC may also suffice if supplemental data from a characterized FACS or plate-imaging workflow are also included in the package.

Cell lines generated by methods …

Probability Distributions In The Glass Failure Prediction Model, Samir Blanchet, H. Scott Norville, Stephen Morse

Michigan Tech Publications, Part 1

Glass, a brittle material, fractures under tensile stress acting over a time duration. Lateral loads, such as wind, acting on a simply supported rectangular glass lite, put one surface of the lite primarily into tension. ASTM E 1300 defines load resistance of glass as the uniform lateral loading acting over a duration of 3 seconds that is associated with a probability of breakage of 8 lites per 1000 at the first occurrence of the loading. To determine load resistance, the underlying window glass failure prediction model facilitates determination of a probability distribution of 3 second equivalent failure loads, P3. The …

Ftc Regulating Cybersecurity Post Wyndham: An International Common Law Comparison On The Impact Of Regulation Of Cybersecurity, Andrew Z. R. Smith

Georgia Journal of International & Comparative Law

No abstract provided.

Energy Modeling & Design Of Prototype Hydroponic Grow System, Emily Denise Trawick, William James Stinson, Alexander Charles Martin

Senior Honors Projects, 2010-2019

Energy and food security relies on innovations that spur sustainable ideologies. This project considers a novel approach to grow microgreens within a controlled environment in a manner that conserves water, minimizes environmental impacts from agriculture runoff, and enables successful agriculture in virtually any environment. The eQUEST® software package, an energy simulator, has been used to create a model of the “grow box” considered in this study. The dimensions were specified, and heating, cooling, and other loads were incorporated into the model which was used to estimate energy consumption. Real-time data were collected from sensors installed in the container and were …

Atmospheric Resiliency: Enhancing Environmental Air Quality Through Bio-Architecture, Francesca Boakye

Bachelor of Architecture Theses - 5th Year

The theory of purifying a city’s smog filled atmosphere by using green microalgae can be architecturally realized through a designed building envelope composed of multiple units of microalgae filled perforated panels. The nature of this project is bio-architectural. Precise execution of it relies on deep research in vehicular emission pollution in terms of molecular composition. This research will help identify the most prominent pollutant in the smog composition and match it to a species of algae that can completely process it during photosynthesis. In addition to air pollution research, research on algae typology, growth and environmental impact is also essential …

Design Of A Homeopathic Solution For Chronic Cough, Jacob Ziemke

Senior Honors Projects, 2010-2019

Chronic cough is most commonly defined as a cough that persists for more than eight weeks and is estimated to affect more than 30 million people in the United States at any given time. Diseases contributing to the onset of chronic cough include asthma, pulmonary fibrosis, lung cancer, postnasal drip, gastroesophageal reflux disease (GERD), and bronchitis, and may include lifestyle choices such as smoking. For those who seek medical advice, pharmaceuticals and speech therapy are two common methods of combating chronic cough but serve to mask the symptoms rather than treat the problem; frequently, chronic cough is misdiagnosed or cannot …

Sustainable Agriculture: Integration Of Aquaponics At Punta Leona Hotel And Club In Costa Rica, Cailin Sierra Dyer, Paris Riley Smith

Senior Honors Projects, 2010-2019

Sustainable agriculture is becoming an increasingly important method of food production. As human populations continue to grow, attendant food demand has been increasingly met via agribusiness, including monoculture crop production and factory farming. As is well documented, the rise of agribusiness has led to resource degradation and declining stocks on which “sustainable agriculture” relies. This paper describes a local attempt to re-establish “sustainable agriculture” through the development of an aquaculture system that mimics a naturally occurring cycle that integrates fish and plants. The system was constructed over a three-week period in Punta Leona, Costa Rica. First, the ground was cleared …

City Of Tyler Hub-And-Spoke Bicycle Lane Network, Pedro A. Zavagna

Civil Engineering Theses

The purpose of this study is to showcase the up-to-date development of a Bicycle Lane Engineered Scoring System (BLESS) that will be utilized to map and identify the optimum feasible locations for bicycle lanes. The project was initiated for the City of Tyler, Texas where a Hub-and-Spoke bicycle lane will be designed around the city utilizing the BLESS. The design process is comprised of attentive planning, progressive development and a collective effort by the transportation engineering research team at the University of Texas at Tyler to design an exceptional bicycle lane network. The BLESS consisted of traffic analysis, lane configuration, …

The Power Of Coefficients And Methods Of Coding In Delimiting Species Using Phenetic Approach: The Case Of African Solanum Section Solanum Sensu Edmonds, Mkabwa Lk Manoko

Tanzania Journal of Science

Phenetics is one of approaches used to delimit species in plant classification. Conclusion in phenetics is based on overall similarity often of morphological data. The approach uses coded data that are analyzed using coefficients to create similarity matrices that are analyzed using clustering analysis to create the classification. Exists different similarity coefficients and coding methods though in practice are used intuitively sometimes giving results that have been challenged. Though similarity coefficients and coding methods have some times been blamed, studies to analyze their influences are limited. The trend however is to avoid morphological data in favor of DNA markers. The …

Total Electron Content Derived From Global Positioning System During Solar Maximum Of 2012-2013 Over The Eastern Part Of The African Sector, Emmanuel D Sulungu, Christian Bs Uiso, Patrick Sibanda

Tanzania Journal of Science

This work presents results of diurnal, seasonal and latitudinal variations of vertical Total Electron Content (TECv) derived from GPS receivers at four locations, [Dodoma (6.19oS, 35.75oE), Mzuzu (11.43oS, 34.01oE), Zomba (15.38oS, 35.33oE) and Tete (16.15oS, 33.58oE)] during the solar maximum period of 2012 – 2013. The receivers are located directly below the EIA and at approximately the same longitude, ~ (33 – 3 oE) within the eastern part of the African sector. Diurnal and latitudinal variations of TECv are presented for an average of the five (5) quietest days of each of the four seasons: March equinox, June solstice, September …

Tolerance Sensitivity Analysis And Robust Optimal Design Method Of A Surface-Mounted Permanent Magnet Motor By Using A Hybrid Response Surface Method Considering Manufacturing Tolerances, Cha-Seung Jun, Byung-Il Kwon, Ohbong Kwon

Publications and Research

This paper presents a robust optimal design method using a hybrid response surface method (H-RSM) which directly finds an optimal point satisfying a target Z-value or a probability of failure. Through three steps, this paper achieves the goal that is to increase the open-circuit airgap flux (OCAF) in a surface-mounted permanent magnet motor and decrease its variation caused by variations of the airgap lengths including an additional one between permanent magnets and rotor back yoke. First, the OCAF equation is derived from the magnetic equivalent circuit (MEC) considering the additional airgap. Then, the equation is validated by comparing its results …

Determinants Of Multi-Scale Patterning In Growth Plate Cartilage, Alek Erickson

Theses & Dissertations

ABSTRACT

Functional architectures of complex adaptive systems emerge by dynamic control over properties of individual components. During skeletal development, growth plate cartilage matches bone geometries to body plan requisites by spatiotemporally regulating chondrocyte actions. Bone growth potential is managed by the proximodistal patterning of chondrocyte populations into differentiation zones, while growth vectors are specified by the unique columnar arrangement of clonal groups. Chondrocyte organization at both tissue and cell levels is influenced by a cartilage-wide communication network that relies on zone-specific release and interpretation of paracrine signals. Despite genetic characterization of signaling interactions necessary for cartilage maturation, the regulatory mechanisms …

A Study Of Acoustically Activated Nanodroplets, Songita Choudhury

Theses & Dissertations

Current treatment of acute myocardial infarction (AMI), which is the main pathophysiological event leading to death in the United States, has advanced considerably with the introduction of emergent percutaneous interventions, but there remains an urgent need for novel techniques to rapidly and accurately detect infarcted or ischemic tissue that results from AMI. Ultrasound contrast agents, also known as microbubbles (MB), have become commonplace in echocardiography. However, MBs are purely intravascular tracers and unable to cross endothelial barriers due to size. The limitations of MBs, namely size and short circulation times within the human body, led to the development of phase-change …

The Viability Of Quantum Computing, Brennan Michael King

Missouri S&T’s Peer to Peer

Quantum computing is an upcoming computational technology that could be the key to advancing the field and ushering in a new era of innovation. In this paper examines the viability of quantum computing extensively using only highly credible peer-reviewed articles from the last few years. These peer-reviewed articles will provide relevant facts and data from prominent researchers in the field of computer engineering. A growing problem in the field of electronics and computers is the concept of Moore’s law. Moore’s law refers to the doubling of transistors every two years in integrated circuits. Recent research has suggested that electronics may …

Experiences Building, Training, And Deploying A Chatbot In An Academic Library, David Meincke

Library Staff Publications

No abstract provided.

Analysis Of The Contribution Of Transportation And Land Use To Citizen Perceptions Of Livability, Rebecca Lewis

PSU Transportation Seminars

What is livability? How does the built environment influence resident perceptions of livability? Although livability is a broadly used term and a key goal in land use and transportation plans at the state level, it is unclear whether residents think their neighborhoods are livable and what contributes to their perception of livability. The purpose of the project was to understand how Oregonians, in neighborhoods of varying densities and within Metropolitan Planning Organizations (MPOs), perceive livability at the nexus of transportation and land use. We sought to understand how residents define and perceive livability in three different MPOs in the state: …

Thermal Comparison Of Polycrystalline Diamond And Aln In Power Semiconductor Device Packages, Brandon M. Guenther

Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research

The desire for improved thermal management in power semiconductor device packaging is becoming increasingly important due to the progression towards implementing wide-bandgap semiconductor (WBG) materials, such as silicon carbide (SiC) and gallium nitride (GaN). These semiconductor materials have the capability of operating at much higher voltages, temperatures, and frequencies compared to standard silicon-based devices. However, utilizing this enhanced operating region will induce larger thermomechanical stress within the package structure as a consequence of operating at higher junction temperatures around 250-300 °C. To handle the higher and improved operating characteristics from the WBG semiconductors, the current package technology is modified by …

Nmr Studies Of Loaded Microspheres, Ming Huang, Sisi Chen, Rex E. Gerald Ii, Jie Huang, Klaus Woelk

Electrical and Computer Engineering Faculty Research & Creative Works

Porous-wall hollow glass microspheres (PWHGMs) are a novel form of glass materials that consist of 1-μm-thick porous silica shells, 20-100 μm in diameter, with a hollow cavity in the center. Utilizing the central cavity for material storage and the porous walls for controlled release is a unique combination that renders PWHGMs a superior vehicle for targeted drug delivery. In this study, NMR spectroscopy was used to characterize PWHGMs for the first time. A vacuum-based loading system was developed to load PWHGMs with various compounds followed by a washing procedure that uses solvents immiscible with the target material. Immiscible binary model …

Utah Salt Storage Facilities Guidelines And Best Management Practices, Weston Bellon

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

An environmental analysis was conducted at the Utah Water Research Laboratory of samples gathered from Utah Department of Transportation (UDOT) salt storage facilities retention ponds across the state. The analysis indicated high levels of TSS largely made up of minerals directly associated with the deicing solution currently used by UDOT in roadway applications. As a companion to that analysis, best management practices have been identified and compiled to provide a resource for the proper management of salt storage facilities in the state of Utah. Topics included as best management practices include locating new storage facilities, proper salt storage, proper salt …

Research And Development Of Powder Brazing Filler Metals For Diamond Tools: A Review, Fei Long, Peng He, Dusan P. Sekulic

Mechanical Engineering Faculty Publications

Powder brazing filler metals (PBFMs) feature a number of comparative advantages. Among others, these include a low energy consumption, an accurate dosage, a good brazeability, a short production time, and a high production efficiency. These filler metals have been used in the aerospace, automobile, and electric appliances industries. The PBFMs are especially suitable for diamond tools bonding, which involves complex workpiece shapes and requires accurate dosage. The recent research of PBFMs for diamond tools is reviewed in this paper. The current applications are discussed. The CuSnTi and Ni-Cr-based PBFMs have been the two commonly used monolayer PBFMs. Thus, the bonding …

Study Of Near-Cup Droplet Breakup Of An Automative Electrostatic Rotary Bell (Esrb) Atomizer Using High-Speed Shadowgraph Imaging, Jacob E. Wilson, Stephen William Grib, Adnan Darwish Ahmad, Michael W. Renfro, Scott A. Adams, Ahmad Salaimeh

Mechanical Engineering Faculty Publications

Electrostatic Rotary bell (ESRB) atomizers are used as the dominant means of paint application by the automotive industry. They utilize the high rotational speed of a cup to induce primary atomization of a liquid along with shaping air to provide secondary atomization and transport. In order to better understand the fluid breakup mechanisms involved in this process, high-speed shadowgraph imaging was used to visualize the edge of a serrated rotary bell at speeds varying between 5000 and 12,000 RPM and with a water flow rate of 250 ccm. A multi-step image processing algorithm was developed to differentiate between ligaments and …

Geoscan-M Use At Ma’Aden Phosphate Al Jalamid Mine, Saudi Arabia, Luke Balzan, Zoran Bauk, Andrew Harris

Beneficiation of Phosphates VIII

Access to real-time data is critical for process control. Scantech’s GEOSCAN-M elemental analyser has been utilized in the minerals industry for the past two decades in a range of different commodities. Already established for use in the coal and cement industries, commodities such as iron ore, copper, lead zinc, manganese, bauxite and lithium have benefited from the real-time access to elemental composition data on conveyed flows provided by the GEOSCAN. The phosphate industry has also recognized the benefits of the equipment and a number of operators and companies have installed GEOSCANs to provide real-time analysis back to the plant.

A …

The Santa Clara, 2018-05-03, Santa Clara University

The Santa Clara

No abstract provided.

Can Your P2o5 Be Commercially Exploited?, Ewan Wingate, Michael E. Kelahan, M. Robert Kelahan

Beneficiation of Phosphates VIII

World reserves of high-grade, high-quality phosphate rock are steadily decreasing. As a result, lower quality rock is being increasingly evaluated to determine its potential for commercial exploitation. It is well known that the chemical and mineralogical composition of phosphate rock varies widely from deposit to deposit, and therefore, the conditions for processing different phosphate rock types also varies.

Unfortunately, most early stage phosphate feasibility studies tend to focus on the P₂O₅ content and neglect the other chemical constituents or impurities in the rock. It is the remainder of the chemical constituents that often define the quality of …

Milling Of The Phosphate Rock Flotation Circuit Circulating Load Aiming Production Increase And Iron Content Reduction In The Final Concentrate, André Carlos Silva, Michelle Fernanda De Lira Teixeira, Anastácio Honório De Melo Filho, Thiago Drumond De Alvarez De Araújo, Maurício José De Oliveira Júnior, Wanderson Ferreira Borges Júnior, Bruno Palhares Milanezi, Jailson Pinto Cardoso

Beneficiation of Phosphates VIII

Phosphorus has its main application in the production of phosphoric acid, being the apatite series its main ore. The Brazilian main phosphorus reserves are igneous, as is the case of Catalão alkaline dome, were Copebras/CMOC International has operations. The content of P₂O₅ in the ROM has been reduced over the years and, as a consequence, the content of the contaminants in the concentrate, produced by flotation, which enhances the difficulty in the phosphoric acid production. Only traces of arsenic, iron, and heavy metals can be present in high-purity phosphoric acid. The circulating load of the Copebras’ phosphate …