Open Access. Powered by Scholars. Published by Universities.®

Engineering Commons™

Open Access. Powered by Scholars. Published by Universities.®

Discipline
Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 76951 - 76980 of 195925

Full-Text Articles in Engineering

Electric Aircraft And The Environment: A Literature Review, Uyen T. Sou May 2018

Electric Aircraft And The Environment: A Literature Review, Uyen T. Sou

McNair Research Journal SJSU

Historically, the integration of social need, environmental impact, and technological advancement has been a challenging balancing act for growing cities and metropoles. If the balance between social need and environmental sustainability is reached, the increasing advancements and improvements made to electric aircrafts have the potential to steer society towards a new mode of sustainable air. One reason why electric aircraft technology has not grown as fast as other technologies is because there lacked a need for fast advancements. With that said, electric aircraft technologies are improving today due to the demand in heavily populated cities to relieve traffic [5]. Some …

Go to article

Finite Element Analysis Of A Guideway For Automated Transit Networks, Issac Gendler May 2018

Finite Element Analysis Of A Guideway For Automated Transit Networks, Issac Gendler

McNair Research Journal SJSU

An asymmetric beam currently being utilized in a solar powered automated transit system was analyzed for its deflections, stresses, and angle of twist. Finite element analysis (FEA) with ANSYS was used in conjunction with hand calculations from beam theory to determine the response of the guideway to loading anticipated in normal operation. An iterative approach was used for modeling the system, where the geometry was taken from a simplified case and progressed in complexity until the original model was duplicated. After analysis, the deflections, stresses, and angles of twist were found to be within suitable ranges for a suspended transportation …

Go to article

Advances In Cell Line Engineering And Protein Expression Strategies, Steven Lang, Diane Hatton May 2018

Advances In Cell Line Engineering And Protein Expression Strategies, Steven Lang, Diane Hatton

Cell Culture Engineering XVI

No abstract provided.

Go to article

Cell Culture Bioprocess Learnings: Past Successes And Future Challenges, Günter Jagschies May 2018

Cell Culture Bioprocess Learnings: Past Successes And Future Challenges, Günter Jagschies

Cell Culture Engineering XVI

Therapeutic proteins, vaccines and – further away – cell and gene therapies are considered essential to find efficacious interventions and preventive measures to a rather long list of prioritized needs in healthcare. The challenge is developing into a ‘tsunami’ of sorts as the global demographic transition causes populations to age, dramatically accelerating the growth of non-communicable disease (NCD) in all parts of the world. India and China are the markets with the largest patient population for almost any NCD, or they are going to be in that position soon. Competition in this field is ramping up with an intensity that …

Go to article

Human Factors And The Road To Single Pilot Operations, Michael Gearhart May 2018

Human Factors And The Road To Single Pilot Operations, Michael Gearhart

Honors Program Theses and Projects

The purpose of this research paper is to shed light on the feasibility and implications concerning the introduction of single-pilot operations on air transport category aircraft. As technology has been advancing over the past few decades, autonomous artificial intelligence software has started to become more popular, finding higher demands in various industries. With current issues such as the pilot shortage and raising pilot wages, airlines are looking for ways to cut down on costs and preserve a healthy work force level. The implementation of this artificial intelligence technology on the aircraft flight deck provides a potential solution to this problem. …

Go to article

Ipsc-Derived Neurospheroids Recapitulate Development And Pathological Signatures Of Human Brain Microenvironment, Catarina Brito, Ana Paula Terrasso, Daniel Simão, Francisca Arez, Marta M. Silva, Patrícia Gomes-Alves, Paula M. Alves, Marcos F. Sousa, Nuno Raimundo, Neus Bayó-Puxan, Sophie Creysells, Eric J. Kremer May 2018

Ipsc-Derived Neurospheroids Recapitulate Development And Pathological Signatures Of Human Brain Microenvironment, Catarina Brito, Ana Paula Terrasso, Daniel Simão, Francisca Arez, Marta M. Silva, Patrícia Gomes-Alves, Paula M. Alves, Marcos F. Sousa, Nuno Raimundo, Neus Bayó-Puxan, Sophie Creysells, Eric J. Kremer

Cell Culture Engineering XVI

Brain microenvironment plays an important role in neurodevelopment and pathology, where extracellular matrix (ECM) and soluble factors modulate multiple cellular processes. Neural cell culture typically relies on the use of heterologous matrices that poorly resemble the brain ECM or reflect its pathological features. We have previously demonstrated that perfusion stirred-tank bioreactor-based 3D differentiation of human neural stem cells (NSC) - pSTR-neurospheroids, sustains the concomitant differentiation of the three neural cell lineages (neurons, astrocytes and oligodendrocytes) and the establishment of physiologically relevant cell-cell interactions. Here, we hypothesized that if the pSTR-neurospheroid strategy would also allow the deposition of native neural ECM …

Go to article

Congratulations 2018 Graduates, Usu College Of Engineering May 2018

Congratulations 2018 Graduates, Usu College Of Engineering

College of Engineering News

The College of Engineering at Utah State University congratulates all students who graduated May 4-5.

Go to article

Design And Synthesis Of Novel Nanometallic Catalysts And Electrode Materials For Green Fuel Cells, Jing Zhang May 2018

Design And Synthesis Of Novel Nanometallic Catalysts And Electrode Materials For Green Fuel Cells, Jing Zhang

Graduate Dissertations and Theses

The United Nations formally adopted 17 sustainable development goals (SDGs) at its 2015 summit. Many of these goals addressed issues such poverty, hunger, health, education, climate-change, gender equality, water, sanitation, energy, urbanization, environment and social justice. The seventh SDG seeks to ensure access to affordable, reliable, sustainable and modern energy for all. So among all renewable forms of energy, fuel cells with high efficiency have attracted a lot of attention recently. In particular, the direct ethanol fuel cells (DEFCs) and microbial fuel cells (MFCs) are significant due to their green fuel, less waste generated and environmental friendliness. Hence the overall …

Go to article

Effect Of Zr Additions On Thermal Stability Of Al-Cu Precipitates In As-Cast And Cold Worked Samples, Kyle Deane, Paul G. Sanders May 2018

Effect Of Zr Additions On Thermal Stability Of Al-Cu Precipitates In As-Cast And Cold Worked Samples, Kyle Deane, Paul G. Sanders

Michigan Tech Publications, Part 1

While Zr is frequently added to Al alloys to control grain size with the formation of large (>1 μm) primary precipitates, little research has been conducted on the effect of nanoscale Al3Zr precipitates on Al alloys. By comparing the precipitation and corresponding strength evolution between Al-Cu-Zr alloys with different Zr concentrations, the effects of Zr on Al-Cu precipitation with and without primary Al3Zr precipitates can be observed. In the absence of these large precipitates, all Al3Zr phases can be formed, through high temperature aging treatments, as a dispersion of nanoprecipaites inside the Al …

Go to article

Fn3 Proteins Engineered To Recognize Tumor Biomarker Mesothelin Internalize Upon Binding, Allison R. Sirois, Daniela A. Deny, Samantha R. Baierl, Katia S. George, Sarah J. Moore May 2018

Fn3 Proteins Engineered To Recognize Tumor Biomarker Mesothelin Internalize Upon Binding, Allison R. Sirois, Daniela A. Deny, Samantha R. Baierl, Katia S. George, Sarah J. Moore

Engineering: Faculty Publications

Mesothelin is a cell surface protein that is overexpressed in numerous cancers, including breast, ovarian, lung, liver, and pancreatic tumors. Aberrant expression of mesothelin has been shown to promote tumor progression and metastasis through interaction with established tumor biomarker CA125. Therefore, molecules that specifically bind to mesothelin have potential therapeutic and diagnostic applications. However, no mesothelin-targeting molecules are currently approved for routine clinical use. While antibodies that target mesothelin are in development, some clinical applications may require a targeting molecule with an alternative protein fold. For example, non-antibody proteins are more suitable for molecular imaging and may facilitate diverse chemical …

Go to article

Mir-Catch Identifies Biologically Active Mirna Regulators Of The Pro-Survival Gene Xiap, In Chinese Hamster Ovary Cells, Paul S. Kelly, Alan Griffith, Sebastian Vencken, Nga T. Lao, Martin Clynes, Niall Barron May 2018

Mir-Catch Identifies Biologically Active Mirna Regulators Of The Pro-Survival Gene Xiap, In Chinese Hamster Ovary Cells, Paul S. Kelly, Alan Griffith, Sebastian Vencken, Nga T. Lao, Martin Clynes, Niall Barron

Cell Culture Engineering XVI

Genetic engineering of mammalian cells, in particular Chinese hamster ovary (CHO) cells, is of critical interest to the biopharmaceutical industry as a means to further boost the yields of therapeutic proteins. Complimentary to already in place advanced bioprocesses, stable overexpression of the pro-survival X-linked inhibitor of apoptosis (XIAP) is one example of the successful manipulation of CHO cell genetics resulting in prolonged culture survival, ultimately increasing recombinant protein productivity. However, saturation or burdening of the cells translational machinery can occur in instances of forced expression of a trans-gene thereby achieving the anticipated cellular phenotype without the associated improvement in productivity. …

Go to article

Aligned Carbon Nanotube Arrays Bonded To Solid Graphite Substrates: Thermal Analysis For Future Device Cooling Applications, Betty T. Quinton, Levi Elston, James D. Scofield, Sharmila M. Mukhopadhyay May 2018

Aligned Carbon Nanotube Arrays Bonded To Solid Graphite Substrates: Thermal Analysis For Future Device Cooling Applications, Betty T. Quinton, Levi Elston, James D. Scofield, Sharmila M. Mukhopadhyay

Mechanical and Materials Engineering Faculty Publications

Carbon nanotubes (CNTs) are known for high thermal conductivity and have potential use as nano-radiators or heat exchangers. This paper focuses on the thermal performance of carpet-like arrays of vertically aligned CNTs on solid graphite substrates with the idea of investigating their behavior as a function of carpet dimensions and predicting their performance as thermal interface material (TIM) for electronic device cooling. Vertically aligned CNTs were grown on highly oriented pyrolytic graphite (HOPG) substrate, which creates a robust and durable all-carbon hierarchical structure. The multi-layer thermal analysis approach using Netzsch laser flash analysis system was used to evaluate their performance …

Go to article

Irradiation Experiment For Living Insect-Based Radiological Dispersal Device, Braden Goddard, Walid Metwally, Ahmad Ababneh May 2018

Irradiation Experiment For Living Insect-Based Radiological Dispersal Device, Braden Goddard, Walid Metwally, Ahmad Ababneh

International Journal of Nuclear Security

People generally associate fear with “nuclear”, “radioactive” and “insects”. It is speculated that a release of radioactive living insects would instill more fear into the general public than a “traditional” style radiological dispersion device (RDD). This paper evaluates the potential threat of an insect-based RDD using experimental data. The results of this project found that insect-based RDDs are an insignificant threat due to the challenges in making insects radioactive enough to pose any danger to humans without killing the insects.

Go to article

Driver Responses To Graphic-Aided Portable Changeable Message Signs In Highway Work Zones, Yilei Huang, Yong Bai May 2018

Driver Responses To Graphic-Aided Portable Changeable Message Signs In Highway Work Zones, Yilei Huang, Yong Bai

Civil and Environmental Engineering Faculty Research and Publications

Portable changeable message signs (PCMSs) have been employed in highway work zones as temporary traffic control devices. Various studies showed that adding graphics to PCMS messages can provide advantages to traditional text messages, such as increasing legibility and improving the understanding of elderly drivers. This article synthesizes the findings of a two-phase research project aimed to investigate driver responses to graphic-aided PCMSs. Different text and graphic-aided PCMSs representing roadwork and flagger were set up in the upstream of highway work zones, and speed data of more than 2,700 vehicles were collected with a series of five speed sensors to determine …

Go to article

Growth Of An Algae Polyculture And Dunaliella Tertiolecta In Oilfield Produced Water At A Range Of Salinities And Nutrient Concentrations For Biofuels Production., Thomas C. Hopkins May 2018

Growth Of An Algae Polyculture And Dunaliella Tertiolecta In Oilfield Produced Water At A Range Of Salinities And Nutrient Concentrations For Biofuels Production., Thomas C. Hopkins

Civil Engineering ETDs

Growth of algae in saline produced waters (wastewater) from oil and gas production is of interest for potential production of algal biofuels, wastewater treatment and potential reuse or disposal. The objectives of this study were to determine the effects of salt concentrations and nitrogen source (ammonia or nitrate) and concentration on algal growth, lipid production, and nutrient removal to help determine suitability for growth on saline produced waters. Dunaliella tertiolecta (a marine algae) and a polyculture (previously discovered growing in PW) were grown on produced water with a range of salinities and varying nitrogen source and concentration, in a series …

Go to article

Investigation Of The Use Of A Bio-Derived Solvent For Non-Solvent-Induced Phase Separation (Nips) Fabrication Of Polysulfone Membranes, Xiaobo Dong, Amna Al-Jumaily, Isabel Escobar May 2018

Investigation Of The Use Of A Bio-Derived Solvent For Non-Solvent-Induced Phase Separation (Nips) Fabrication Of Polysulfone Membranes, Xiaobo Dong, Amna Al-Jumaily, Isabel Escobar

Chemical and Materials Engineering Faculty Publications

Organic solvents, such as N-methyl-2-pyrrolidone (NMP) and dimethylacetamide (DMAc), have been traditionally used to fabricate polymeric membranes. These solvents may have a negative impact on the environment and human health; therefore, using renewable solvents derived from biomass is of great interest to make membrane fabrication sustainable. Methyl-5-(dimethylamino)-2-methyl-5-oxopentanoate (Rhodiasolv PolarClean) is a bio-derived, biodegradable, nonflammable and nonvolatile solvent. Polysulfone is a commonly used polymer to fabricate membranes due to its thermal stability, strong mechanical strength and good chemical resistance. From cloud point curves, PolarClean showed potential to be a solvent for polysulfone. Membranes prepared with PolarClean were investigated in terms of …

Go to article

Validation Of Cfd Simulations For Hypersonic Flow Over A Yawed Cone, Julian Cecil May 2018

Validation Of Cfd Simulations For Hypersonic Flow Over A Yawed Cone, Julian Cecil

McKelvey School of Engineering Graduate Student Theses & Dissertations

This study aims to numerically simulate the wind tunnel results for hypersonic flow over a circular cone of semi-apex angle of 10 degrees yawed from 0° to 20° using the commercial computational fluid dynamics software ANSYS Fluent. The ANSYS workbench is used to create the 10° semi-apex circular cone with a shock aligned structured mesh of 3.05 million cells surrounding the cone. Simulation boundary conditions for pressure and temperature in the far field correspond to Tracy’s wind tunnel experiment at Cal Tech. The six simulations cases are conducted for yaw angles of 0, 8, 12, 16, 20 and 24 degrees. …

Go to article

Pedestrian Injury Severity Analysis In Motor Vehicle Crashes In Ohio, Majbah Uddin, Fahim Ahmed May 2018

Pedestrian Injury Severity Analysis In Motor Vehicle Crashes In Ohio, Majbah Uddin, Fahim Ahmed

Faculty Publications

According to the National Highway Traffic Safety Administration, 116 pedestrians were killed in motor vehicle crashes in Ohio in 2015. However, no study to date has analyzed crashes in Ohio in order to explore the factors contributing to the pedestrian injury severity resulting from motor vehicle crashes. This study fills this gap by investigating the crashes involving pedestrians exclusively in Ohio. This study uses the crash data from the Highway Safety Information System, from 2009 to 2013. The explanatory factors include the pedestrian, driver, vehicle, crash, and roadway characteristics. Both fixed- and random-parameters ordered probit models of injury severity (where …

Go to article

Controlling Continuous High Cell Density Perfusion Culture With The Alternating Tangential Flow System In Real Time Using Radio-Frequency Impendance, Aditya Bhat May 2018

Controlling Continuous High Cell Density Perfusion Culture With The Alternating Tangential Flow System In Real Time Using Radio-Frequency Impendance, Aditya Bhat

Cell Culture Engineering XVI

Many cGMP cell culture processes are now based on a continuous high cell density perfusion bioreactor system. Control of the feed or addition rates to maintain pseudo-steady-state conditions in these bioreactors can be especially challenging due to high and fluctuating cell concentrations that can rapidly change environmental conditions. With infrequent manual daily sampling based on offline cell counting, the control system can have too little information on which to base an appropriate decision to manipulate the process. Tight control of the perfusion or concentrate addition rate allows the bioreactor to be operated under the optimum conditions for maximum recombinant protein …

Go to article

Scalable Lentiviral Vector Production Using Stable Producer Cell Lines In Perfusion Mode, Sven Ansorge, Aziza Manceur, Stéphane Lanthier, Sonia Tremblay, Anne-Marie Gélinas, July Dorion-Thibaudeau, Sophie Broussau, Julia Transfiguracion, Hafida Aomari, Rénald Gilbert May 2018

Scalable Lentiviral Vector Production Using Stable Producer Cell Lines In Perfusion Mode, Sven Ansorge, Aziza Manceur, Stéphane Lanthier, Sonia Tremblay, Anne-Marie Gélinas, July Dorion-Thibaudeau, Sophie Broussau, Julia Transfiguracion, Hafida Aomari, Rénald Gilbert

Cell Culture Engineering XVI

Lentiviral vectors (LVs) are becoming an important tool in gene and cell therapy and are being utilized in several clinical studies against genetic and acquired diseases, as well as in cancer therapies. To address the challenges linked to the generation of preclinical and clinical supply, the National Research Council Canada has developed packaging cell lines and stable producer cell lines for the production of LVs which can grow in suspension in serum-free media and produce LV in the 106 TU/ml range without optimization. We focus on the development of perfusion processes to both intensify the process and harvest produced LV …

Go to article

Dxb11/Taut Host Cell Engineering Strategy Enabling The Establishment Of Strains Producing The Highest Yield Of Advanced Recycling Antibodies, Hisahiro Tabuchi, Masaki Yamaguchi, Tao Jialin, Hirokatsu Makitsubo May 2018

Dxb11/Taut Host Cell Engineering Strategy Enabling The Establishment Of Strains Producing The Highest Yield Of Advanced Recycling Antibodies, Hisahiro Tabuchi, Masaki Yamaguchi, Tao Jialin, Hirokatsu Makitsubo

Cell Culture Engineering XVI

Innovation in monoclonal antibody (mAb) production for clinical use continues to be driven by cell engineering strategies to increase mAb yield and to control the complexity of advanced recycling antibodies (rcAbs). rcAbs offer significant advantages for efficacy by dissociating the antigens in endosomes and recycling free antibodies back to plasma. We were able to reduce the instability in an rcAb-producing CHO cell line whereby mAb titer during the stage of cell line development (CLD) was decreased and improve cell culture optimization. In this study, we used a chemically defined medium (CDM) and CDM-adapted transporter-overexpressing DXB11 host cells for CLD of …

Go to article

Novel Transposase Tools For Cell-Line Engineering, Ferenc Boldog, Jeremy Minshull, Mark Welch, Kate Caves May 2018

Novel Transposase Tools For Cell-Line Engineering, Ferenc Boldog, Jeremy Minshull, Mark Welch, Kate Caves

Cell Culture Engineering XVI

ATUM has discovered, characterized, engineered and patented new transposases that work comparable to, or better than previously commercialized transposases. The technology is highly valuable for protein expression and genome engineering applications. It enables a specified sequence to behave as a transposon, a mobile genetic element, which can efficiently transpose between vectors and chromosomes via a “cut & paste” mechanism. The Leap-In Transposase® catalyzes the integration of a transposon containing your gene into TTAT sites in the target genome. During transposition, the Leap-In Transposase recognizes transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and moves …

Go to article

Empowering Manufacturing Decisions Through Process Simulation Models, Tiffany Rau, Terence Davidovits, Rick Stock May 2018

Empowering Manufacturing Decisions Through Process Simulation Models, Tiffany Rau, Terence Davidovits, Rick Stock

Cell Culture Engineering XVI

The need for Clinical and Commercial manufacturing capacity continues to increase and at the same time there are “shortages” of production slots and time pressures. Whether one is a virtual, small or large company there are many considerations that go into the decision of how and where to manufacture the biopharmaceutical, cell therapy or vaccine. In this presentation, we explore approaches for using commercially available modeling tools, such as Super ProDesigner® and BioSolve™, to simulate the manufacturing of a “typical” monoclonal antibody product and a multivalent conjugate vaccine product. Through these hypothetical case studies, we will illustrate several important concepts …

Go to article

Conference Program, Anne Skaja Robinson, Raghavan Venkat, Gene Schaefer May 2018

Conference Program, Anne Skaja Robinson, Raghavan Venkat, Gene Schaefer

Cell Culture Engineering XVI

No abstract provided.

Go to article

Impact Of Raw Materials On Sialyation For A Therapeutic Protein, Wei-Chien Hung, Mei Shao, Bridget Leslie, John Mavrianos, Paul Loray, Mei Shao May 2018

Impact Of Raw Materials On Sialyation For A Therapeutic Protein, Wei-Chien Hung, Mei Shao, Bridget Leslie, John Mavrianos, Paul Loray, Mei Shao

Cell Culture Engineering XVI

Total sialic acid content (TSAC) is a critical product quality attribute (CQA) for a therapeutic protein. Previous studies and results have suggested lot-to-lot variability of raw material, media powder and feed, have potential effects on total sialyation acid content (TSAC) at cell culture fluid (CCF) and harvested cell culture fluid (HCCF). Through searching literature, we found that potential components in media powder and feed such trace metal and vitamins can greatly modulate the sialyation acid level in vivo or in vitro. For instance, the sialic acid content was simultaneously increased in glycopeptides as well as gangliosides under lithium treatment1 …

Go to article

Demonstrating A Powerful Scale-Up Strategy For Biosimilar Mab In Single Use Systems Via Physicochemical And Functional Characterization, Serdar Alpan, Ozge Can, Deniz Baycin, Yigit Erdemgil, Zeynep Yildirim Keles, A. Emin Atik May 2018

Demonstrating A Powerful Scale-Up Strategy For Biosimilar Mab In Single Use Systems Via Physicochemical And Functional Characterization, Serdar Alpan, Ozge Can, Deniz Baycin, Yigit Erdemgil, Zeynep Yildirim Keles, A. Emin Atik

Cell Culture Engineering XVI

Biosimilars have received a remarkable attention in the recent years. Due to the heterogeneity of biosimilar mAbs, they need to be well-characterized by various orthogonal techniques in order to identify their physicochemical and functional characteristics. Characterization of the post translational modifications, especially, glycosylation is vital to define the critical quality attributes (CQAs) which affect safety, efficacy and quality of drugs. In this study, we were able to manipulate the quality of the drug by using scale-up strategies for single use systems. By using ultra-performance liquid chromatography (UPLC) coupled to mass spectrometry (MS), we were able to demonstrate physicochemical similarities between …

Go to article

The Journey From Tech Transfer To Bla Submission: Case Study Of A Ns0 Cell Culture Process From 2000l Stainless Steel Bioreactor To 2000l Disposable Bioreactor, Jincai Li May 2018

The Journey From Tech Transfer To Bla Submission: Case Study Of A Ns0 Cell Culture Process From 2000l Stainless Steel Bioreactor To 2000l Disposable Bioreactor, Jincai Li

Cell Culture Engineering XVI

A case study of NS0 cell culture process transfer from 2000L stainless steel bioreactor (SST) to 2000L disposable bioreactor (SUB), and through to process validation and BLA submission is reported for production of an antibody therapeutics in this poster. Initial attempts in growing the NS0 cells in the small scale 2D bags yielded non-satisfactory results, as growth was impacted by bag material type as well as by different suppliers of the same bag material type. However, 3D bags of 50L and above proved to be supportive of the NS0 cell line growth. Process characterization (PC) and process validation (PV) efforts …

Go to article

Identifying Hipk1 As A Target Of Mir-22-3p Enhancing Recombinant Protein Production From Hek 293 By Using Microarray And Htp Sirna Screen, Sarah Inwood, Eugen Buehler, Michael Betenbaugh, Madhu Lal, Joseph Shiloach May 2018

Identifying Hipk1 As A Target Of Mir-22-3p Enhancing Recombinant Protein Production From Hek 293 By Using Microarray And Htp Sirna Screen, Sarah Inwood, Eugen Buehler, Michael Betenbaugh, Madhu Lal, Joseph Shiloach

Cell Culture Engineering XVI

Enhancing protein production in mammalian cells is of interest in the biomedical field for a variety of reasons, including structural studies and antibody production. Using small non-protein coding RNA such as microRNA has recently been a promising method of increasing protein expression. A high throughput human microRNA screen in HEK 293 cells previously identified miRNA 22-3p as a promising candidate for increasing recombinant protein expression. This microRNA enhanced the expression of luciferase, two hard-to-express membrane proteins and a secreted hFc-fusion protein. In order to explore the mechanisms of this increase in protein production and to understand the intracellular events, we …

Go to article

Towards Model-Based Bioprocess Characterization: A Mathematical Model Of Cell Cycle, Metabolism And Apoptosis Of Mab-Producing Mammalian Cells, António Lima Grilo, Athanasios Mantalaris May 2018

Towards Model-Based Bioprocess Characterization: A Mathematical Model Of Cell Cycle, Metabolism And Apoptosis Of Mab-Producing Mammalian Cells, António Lima Grilo, Athanasios Mantalaris

Cell Culture Engineering XVI

The biologics industry is changing and all players seek competitiveness through process optimization aiming at satisfying the market demand providing a compliant protein in as short time as possible. As blockbuster drugs experience patent expiry, several biosimilars are coming market and competition becomes fiercer. Unlike classical experiments-based optimization, bioprocess modeling is a rational, economically efficient and reliable alternative to deliver an optimized bioprocess. The biological aspects of a bioreactor are diverse and include cells growing and cycling, other cells dying, nutrients being consumed and by-products accumulating, energy production and usage and therapeutic proteins being produced. The understanding of the biology …

Go to article

Impact Of S-Sulfocysteine On Fragments And Trisulfide Bond Linkages In Monoclonal Antibodies, Aline Zimmer, Ronja Seibel, Alisa Schnellbaecher, Susanne Bohl, Maria Wehsling, Sandra Maier, Anne Zeck May 2018

Impact Of S-Sulfocysteine On Fragments And Trisulfide Bond Linkages In Monoclonal Antibodies, Aline Zimmer, Ronja Seibel, Alisa Schnellbaecher, Susanne Bohl, Maria Wehsling, Sandra Maier, Anne Zeck

Cell Culture Engineering XVI

The quality of recombinant proteins such as monoclonal antibodies produced using Chinese hamster ovary cell-based mammalian systems is dependent on many factors, including cell line, process and cell culture media. Due to these factors, the generated product is heterogeneous and may have chemically induced modifications or post-translational modifications that affect antibody stability, functionality and, in some cases, patient safety. This study demonstrates that S-sulfocysteine, a cysteine derivative, can increase the antibody specific productivity in different cell lines cultivated with different processes while minimizing trisulfide linkages in generated mAbs, mainly between heavy and light chain. The supplementation of a cell culture …

Go to article

First Prev Next Last