Engineering Commons^™

Gust-Induced Aerodynamic Performance In Insects' Forward Flapping Flight, Arash Farsani, Ori Stearns, Gal Ribak, Roi Gurka

Physics and Engineering Science

Flapping-wing flight is inherently unsteady, where atmospheric gusts can substantially degrade the aerodynamic performance when their characteristic time scales are comparable to the wingbeat period. This study presents a numerical investigation and time–frequency characterization of gust-induced aerodynamic response of flapping wings of varying size, inspired by the flight of a longhorn beetle, Batocera rufomaculata. Geometrically similar wings spanning the biological range were simulated in forward flight under identical prescribed kinematics and a transient frontal gust with a smoothly ramped profile. Three-dimensional unsteady Reynolds-averaged Navier–Stokes simulations using the shear stress transport k–ω turbulence model were performed to resolve the instantaneous pressure …

Comparative Lca Of Germanium Production From Coal Fly Ash Using Chlorinated Distillation And Solvent Extraction, Dennis Dadzie, Kwame Awuah-Offei, Michael Moats, Nasib Al Habib, Weston Hartzell

Mining Engineering Faculty Research & Creative Works

Germanium is increasingly important for high-tech applications, yet its supply chain is vulnerable, with production limited to a few countries. Although prior research has explored alternative techniques for extracting and recovering germanium, the literature lacks comparative life-cycle evidence that quantifies their environmental burdens and translates findings into actionable (policy) recommendations. This paper presents a cradle-to-gate life-cycle assessment that compares the environmental burdens of producing 1 kg of single-crystal germanium from coal fly ash via chlorinated distillation (CD) and solvent extraction (SX). Under the study's assumptions, CD has lower potential impacts in 15 of 19 impact categories, while both routes show …

Upar-Guided Dendrimer Gel Nanoparticles Reprogram Inflammation To Stabilize Atherosclerotic Plaques, Hsin Yin Chuang, Huari Kou, Yue-Wern Huang, Hu Yang

Biological Sciences Faculty Research & Creative Works

Atherosclerosis is characterized by lipid deposition, chronic inflammation, and apoptosis within the arterial wall, leading to plaque progression and instability. Current lipid-lowering therapies fail to fully address residual cardiovascular risk driven by local inflammation and cell death. Here, we report the development of uPA-functionalized, rapamycin-encapsulated dendrimer nanoparticles (G5PM-uPA/RA) that preferentially accumulate in urokinase plasminogen activator receptor (uPAR)-enriched atherosclerotic plaque, including macrophage- and apoptosis-rich lesion microenvironments. G5PM-uPA/RA was constructed by cross-linking reaction-enabled flash nanoprecipitation in a custom-made multi-inlet vortex mixer, followed by thiol-maleimide conjugation of uPA for uPAR-guided targeting. The nanoparticles demonstrated uniform morphology, favorable stability, and efficient rapamycin loading. In …

Electrospinning Of Covalent Organic Frameworks (Cofs) For Adsorption Of Heavy Metal Ions, Samar A. Salim

Nanotechnology Research Centre

This study reported the synthesis, electrospinning, and heavy metal adsorption of covalent organic frameworks (COFs) integrated into poly(methyl methacrylate) (PMMA) nanofibers. COF-1 and COF-2 were synthesized by solvothermal and reflux techniques, respectively, and subsequently incorporated into PMMA via electrospinning with different loadings (0.1%, 0.5%, and 1.0%). The synthesized PMMA_COF nanofibers were analyzed utilizing X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and diffuse reflectance spectroscopy (DRS). PMMA_COF nanofibers with fiber diameters of 350-450 nm were obtained. The structural and morphological investigations validated the effective integration of COFs into the PMMA matrix, with COF-1 demonstrating superior dispersion …

Pseudodifferential Absorbing Boundary Conditions For Waves, Lauren Taylor

Mechanical Engineering Theses

Absorbing boundary conditions (ABCs) are required to truncate the computational domain when performing Finite Element Analyses of exterior acoustic problems where physical domain is unbounded. ABC is applied on a fictitious boundary containing the scatterer and ideally allows outgoing waves to leave without non-physical reflections. Preventing artificial reflections is essential to benefit from the accuracy of the numerical method used. Otherwise, ABC acts as a reflective surface, and artificial reflections distort the solution in the entire domain which is not recoverable by any type of refinement. Pseudodifferential ABCs were used to describe the Dirichlet-to-Neumann map which maps known boundary values …

Coherent Two-Photon Backscattering And Induced Angular Quantum Correlations In Multiple-Scattered Two-Photon States Of The Light, Nooshin M. Estakhri, Theodore B. Norris

Engineering Faculty Articles and Research

We present the emergence of coherent two-photon backscattering, a manifestation of weak localization, in multiple scattering of maximally entangled pure and fully mixed two-photon states and examine the effect of entanglement and classical correlations. Quantum correlations in backscattering are investigated for finite three-dimensional disordered structures in the weak localization regime, as well as systems of a small number of scatterers with specified spatial arrangements. No assumptions are made about the statistical behavior of the scattering matrix elements. Furthermore, we study the interplay between quantum correlations induced by multiple scattering and the correlations that may be present in the illumination fields, …

Enhanced Intrusion Detection Using Recurrent Neural Networks With Amino Acid Codon Features, Omar Fitian Rashid, Mohammed Ahmed Subhi, Safa Ahmed Abdulsahib, Mohammed Khaleel Hussein, Marwan Ali Albahar

Iraqi Journal for Computer Science and Mathematics

Intrusion Detection Systems (IDS) is the main defense mechanism deployed by the current networks to prevent cyber threats. Recurrent Neural Network (RNN) are also a novel IDS structure that replaces the conventional training and testing mechanism. The strategy encodes network traffic data as biological sequences using amino acid codons in such a fashion that the RNN is capable of effectively analyzing temporal and sequence data patterns. RNN architecture design adopts embedding layers to handle codon representations and Long Short-Term Memory (LSTM) layers to perform sequential data learning, which is followed by a fully connected network to perform classification functions, which …

The Effect Of Ideals In Near Rings With Respect To Zero Divisor And Completely Equiprime Split Graphs, Sarah Hamid Al-Sharifi, Ameer A. J. Al-Swidi

Iraqi Journal for Computer Science and Mathematics

We seek to find several relationships for some of the graphs that were previously studied by us(completely equiprime graph $CEQ_J( N )$) and by other researchers(simple graph $\Gamma $(R)) based on the ideals of the near rings with our new definition, which is the split and non-split of graphs, which determines the consequent priorities in light of that, specifying that according to the properties of the near ring elements and their ideals.

Latent Space Exploration Of Stylegan2-Ada For Fashion Image Synthesis: A Practical Implementation Guide For Moderate-Sized Datasets, Ali H. Shareef, Hajer Ghodhbani, Tarek M. Hamdani, Habib Chabchoub, Adel M. Alimi

Iraqi Journal for Computer Science and Mathematics

\hlThis paper presents the use of StyleGAN2-ADA for fashion image synthesis and editing. We demonstrate that generative adversarial networks (GANs) can generate realistic and diverse fashion images even under moderate data constraints. The model trained on a dataset of 9,000 fashion images achieved a Fréchet Inception Distance (FID) of 10.8, a Garment Structure Accuracy (GSA) of 0.85, a Pattern Continuity Score (PCS) of 0.78, and an Attribute Transfer Accuracy (ATA) averaging 75% across key attributes. These quantitative results confirm the model’s ability to produce high-quality, structurally consistent, and semantically coherent fashion images. Unlike previous studies focusing on …

Explainable Ai In Agriculture: Bridging The Gap Between Technology And Farmer, Jayarekha P, Anitha H M, Sowmya K S, Nalina V, Audithan Sivaraman, Madhu Narasimha

Iraqi Journal for Computer Science and Mathematics

An intelligent and explainable, as well as user-friendly, crop recommendation and yield prediction system is developed to facilitate agricultural decision-making under various tillage methods with the help of Explainable AI. The system utilizes Machine Learning algorithms and calculates yield changes as a relative term with respect to various inputs such as location, month of sowing, years of NT adoption, and soil type using real-time environmental data; accordingly, it recommends crops. The prediction framework, which is based on various regression algorithms, including Linear Regression, Decision Tree, Random Forest, and Gradient Boosting, was trained on the dataset of agronomic and climatic variables. …

Resolution Of Expression Of Concern: Ontology Features-Based Arabic Text Augmentation Using Word2vec, Enas Tariq Khudair, Onsa Lazzez, Mourad Zaied, Tarek M. Hamdani, Ahmed T. Sadiq, Habib Chabchoub, Adel M. Alimi

Iraqi Journal for Computer Science and Mathematics

Editor's Note: This Resolution notice concludes the investigation initiated by the Expression of Concern (DOI: https://doi.org/10.52866/2788-7421.1386) regarding the Original Article (DOI: https://doi.org/10.52866/2788-7421.1302). The publication timeline is as follows: Original Article → Expression of Concern → Resolution.

Resolution of Expression of Concern:

The editorial team of the Iraqi Journal for Computer Science and Mathematics has completed a thorough post-publication review of the above-mentioned article, the supporting data, and the authors’ responses to the queries raised. Upon careful assessment, we have determined that the concerns have been addressed and clarified to the satisfaction of the editors.

We have found …

Resolution Of Expression Of Concern: The Innovative Technique For Obtaining The Solutions Of Jeffery Hamel Nano-Fluids Flow Problem, Haedir Abd Alrazak Namoos, Abeer Majeed Jasim

Iraqi Journal for Computer Science and Mathematics

Editor's Note: This Resolution notice concludes the investigation initiated by the Expression of Concern (DOI: https://doi.org/10.52866/2788-7421.1389) regarding the Original Article (DOI: https://doi.org/10.52866/2788-7421.1303). The publication timeline is as follows: Original Article → Expression of Concern → Resolution.

Resolution of Expression of Concern:

The editorial team of the Iraqi Journal for Computer Science and Mathematics has completed a thorough post-publication review of the above-mentioned article, the supporting data, and the authors’ responses to the queries raised. Upon careful assessment, we have determined that the concerns have been addressed and clarified to the satisfaction of the editors.

We have found …

Resolution Of Expression Of Concern: Software Engineering Approach To Enhancing Privacy Protection: Automated Face Blurring Using Deep Learning In Arab Social Media, Yasmin Makki Mohialden, Nadia Mahmood Hussien, Mostafa Abdulghafoor Mohammed

Iraqi Journal for Computer Science and Mathematics

Editor's Note: This Resolution notice concludes the investigation initiated by the Expression of Concern (DOI: https://doi.org/10.52866/2788-7421.1388) regarding the Original Article (DOI: https://doi.org/10.52866/2788-7421.1317). The publication timeline is as follows: Original Article → Expression of Concern → Resolution.

Resolution of Expression of Concern:

The editorial team of the Iraqi Journal for Computer Science and Mathematics has completed a thorough post-publication review of the above-mentioned article, the supporting data, and the authors’ responses to the queries raised. Upon careful assessment, we have determined that the concerns have been addressed and clarified to the satisfaction of the editors.

We have found …

Resolution Of Expression Of Concern: Quantum Machine And Deep Learning For Medical Image Classification: A Systematic Review Of Trends, Methodologies, And Future Directions, Eman A. Radhi, Mohammed Y. Kamil, Mazin Abed Mohammed

Iraqi Journal for Computer Science and Mathematics

Editor's Note: This Resolution notice concludes the investigation initiated by the Expression of Concern (DOI: https://doi.org/10.52866/2788-7421.1387) regarding the Original Article (DOI: https://doi.org/10.52866/2788-7421.1252). The publication timeline is as follows: Original Article → Expression of Concern → Resolution.

Resolution of Expression of Concern:

The editorial team of the Iraqi Journal for Computer Science and Mathematics has completed a thorough post-publication review of the above-mentioned article, the supporting data, and the authors’ responses to the queries raised. Upon careful assessment, we have determined that the concerns have been addressed and clarified to the satisfaction of the editors.

We have found …

Resolution Of Expression Of Concern: Leveraging Machine Learning For Accurate Prediction Of Nba Player Salaries, Ye Cheng, Yan Song, Mingqi Wang

Iraqi Journal for Computer Science and Mathematics

Editor's Note: This Resolution notice concludes the investigation initiated by the Expression of Concern (DOI: https://doi.org/10.52866/2788-7421.1385) regarding the Original Article (DOI: https://doi.org/10.52866/2788-7421.1275). The publication timeline is as follows: Original Article → Expression of Concern → Resolution.

Resolution of Expression of Concern:

The editorial team of the Iraqi Journal for Computer Science and Mathematics has completed a thorough post-publication review of the above-mentioned article, the supporting data, and the authors’ responses to the queries raised. Upon careful assessment, we have determined that the concerns have been addressed and clarified to the satisfaction of the editors.

We have found …

Resolution Of Expression Of Concern: Improving Road Safety Through Multimodal Deep Learning For Driver Drowsiness Detection, Hadel A. Hussain, Mohammed A. Subhi, Ahmed S. Al Tmeme, Ahmed D. Radhi, Marwan Ali Albahar

Iraqi Journal for Computer Science and Mathematics

Editor's Note: This Resolution notice concludes the investigation initiated by the Expression of Concern (DOI: https://doi.org/10.52866/2788-7421.1384) regarding the Original Article (DOI: https://doi.org/10.52866/2788-7421.1330). The publication timeline is as follows: Original Article → Expression of Concern → Resolution.

Resolution of Expression of Concern:

The editorial team of the Iraqi Journal for Computer Science and Mathematics has completed a thorough post-publication review of the above-mentioned article, the supporting data, and the authors’ responses to the queries raised. Upon careful assessment, we have determined that the concerns have been addressed and clarified to the satisfaction of the editors.

We have found …

Resolution Of Expression Of Concern: Improved Harmony Search Algorithm For Sdn Controller Placement, Yousra Abdul Alsahib S. Aldeen, Ahmed T. Sadiq, Abeer E. Abed, Omar F. Hussain, Syed Hamid Hussain Madni

Iraqi Journal for Computer Science and Mathematics

Editor's Note: This Resolution notice concludes the investigation initiated by the Expression of Concern (DOI: https://doi.org/10.52866/2788-7421.1382) regarding the Original Article (DOI: https://doi.org/10.52866/2788-7421.1249). The publication timeline is as follows: Original Article → Expression of Concern → Resolution.

Resolution of Expression of Concern:

The editorial team of the Iraqi Journal for Computer Science and Mathematics has completed a thorough post-publication review of the above-mentioned article, the supporting data, and the authors’ responses to the queries raised. Upon careful assessment, we have determined that the concerns have been addressed and clarified to the satisfaction of the editors.

We have found …

Resolution Of Expression Of Concern: Exact Solutions Of M-Fractional Complex Ginzburg-Landau Equation Via Two Analytical, Nematollah Kadkhoda, Mojtaba Baymani, Maad M. Mijwil, Mostafa Abotaleb

Iraqi Journal for Computer Science and Mathematics

Editor's Note: This Resolution notice concludes the investigation initiated by the Expression of Concern (DOI: https://doi.org/10.52866/2788-7421.1381) regarding the Original Article (DOI: https://doi.org/10.52866/2788-7421.1267). The publication timeline is as follows: Original Article → Expression of Concern → Resolution.

Resolution of Expression of Concern:

The editorial team of the Iraqi Journal for Computer Science and Mathematics has completed a thorough post-publication review of the above-mentioned article, the supporting data, and the authors’ responses to the queries raised. Upon careful assessment, we have determined that the concerns have been addressed and clarified to the satisfaction of the editors.

We have found …

Resolution Of Expression Of Concern: Artificial Intelligence In Education: Mathematics Teachers’ Perspectives, Practices And Challenges, Mohammad A. Tashtoush, Yousef Wardat, Rommel Al Ali, Shoeb Saleh

Iraqi Journal for Computer Science and Mathematics

Editor's Note: This Resolution notice concludes the investigation initiated by the Expression of Concern (DOI: https://doi.org/10.52866/2788-7421.1379) regarding the Original Article (DOI: https://doi.org/10.52866/ijcsm.2024.05.01.004). The publication timeline is as follows: Original Article → Expression of Concern → Resolution.

Resolution of Expression of Concern:

The editorial team of the Iraqi Journal for Computer Science and Mathematics has completed a thorough post-publication review of the above-mentioned article, the supporting data, and the authors’ responses to the queries raised. Upon careful assessment, we have determined that the concerns have been addressed and clarified to the satisfaction of the editors.

We have found …

Resolution Of Expression Of Concern: A Lightweight U-Net Model For Accurate Skin Lesion Segmentation, Fallah H. Najjar, Karrar A. Kadhim, Farhan Mohamed, Mohd Shafry Mohd Rahim, Asniyani Nur Haidar Abdullah

Iraqi Journal for Computer Science and Mathematics

Editor's Note: This Resolution notice concludes the investigation initiated by the Expression of Concern (DOI: https://doi.org/10.52866/2788-7421.1378) regarding the Original Article (DOI: https://doi.org/10.52866/2788-7421.1230). The publication timeline is as follows: Original Article → Expression of Concern → Resolution.

Resolution of Expression of Concern:
The editorial team of the Iraqi Journal for Computer Science and Mathematics has completed a thorough post-publication review of the above-mentioned article, the supporting data, and the authors’ responses to the queries raised. Upon careful assessment, we have determined that the concerns have been addressed and clarified to the satisfaction of the editors.
We have found …

The Arching Analysis Of Rigid Inclusion System Using Cohesive Fill For Load Transform Platform, Ramy Mohammed Mohammed Khalil, Amr F. Elhakim

HBRC Journal

Rigid Inclusion System (RIS) is one of the economic systems for improving highly compressible soil by increasing the bearing capacity of foundation soil and reducing settlements. This system consists of a load transform platform (LTP) with or without geosynthetic reinforcement (GR) membrane resting on an arrangement of piles. The LTP redistributes the fill embankment load so that a higher percentage of load is transferred to pile by arching that leads to a reduction in the settlement of the foundation soil. Many analytical, numerical and experimental studies to model arching within LTP using cohesionless fill are summarized. This paper presents an …

Effect Of Recycled Water As Mixing And Curing Water, On Concrete Properties, Nagah Mousalem, Sayed Abdelbaky, Hesham Ali Ezz-Eldeen, Ahmed Abdel Hafiz Mahmoud

HBRC Journal

This research aims to study the effect of secondary treated wastewater (STWW) and tertiary treated wastewater (TTWW) in concrete mixing and curing as a complete alternative to fresh water to address the water crisis. The treated wastewater used in the study was collected from two wastewater treatment plants in Orasqualia for STWW and El Shorouk for TTWW, Egypt. Chemical analysis was performed on the collected water samples to verify their compliance with the recommendations of the relevant standards used in concrete production. Physical and mechanical tests were performed on cement paste, mortar and concrete specimens. Some durability characteristic of concrete …

Improving Heat Transfer Properties Of Corundum Alumina-Based Nanofluids: A Comprehensive Study On Thermal Conductivity And Characterization, Amri Abdulah, Budi Kristiawan, Wibawa Endra Juwana, Sukarman Sukarman, Khoirudin Khoirudin

HBRC Journal

The thermal conductivity of nanofluids is affected by various factors. Consequently, a comprehensive investigation into nanofluid materials and their thermal conductivity is essential. This study examines Al₂O₃ samples with three different volume concentrations, 0.5, 0.75 and 1 vol.%, dispersed in a liquid water/ethylene glycol mixture with a volume ratio of EG–W (50:50). Multi-technique characterization was performed using XRD, SEM-EDS, TEM, UV-Visible, and Zeta Potential. Furthermore, thermal conductivity tests were performed on Al₂O₃ using TEMPOS TEM-1443. The XRD pattern revealed that the Al₂O₃ nanoparticles possess a trigonal crystal system with a rhombohedral …

Divergent Risk Prioritization And Safety Governance In Metro Construction Projects: Evidence From Cairo Metro Line 3, Shrouk M. El Gebaly, Khaled M. Dewidar, Taher M. Elsamni

HBRC Journal

Safety risk management remains a critical challenge in large-scale infrastructure projects, particularly in complex urban metro systems where multiple stakeholders operate under different organizational mandates. While previous research has examined construction safety risks, limited studies have explored how project stakeholders prioritize these risks within the same project environment and how such differences influence safety governance. This study investigates stakeholder risk prioritization in metro construction through a qualitative case study of Cairo Metro Line 3 in Egypt. Data were collected through semi-structured interviews with representatives from three key stakeholder groups: the client organization, consulting engineers, and construction contractors. The data were …

Policy Analysis Of The Inclusion Of Mitigation Measures In The Re-Design And Construction Of The New Frances Scott Key Bridge, Patrick John O'Connor

Theses and Dissertations

This thesis discusses the tragic event of the container ship MV Dali impacting the Francis Scott Key Bridge on March 26, 2024, leading to the collapse of the structure and killing six people despite having collision mitigation protections in place. This research examines how late twentieth-century structures are inadequate for current shipping demands and emphasizes that updated mitigation design criteria must accommodate larger modern vessels as governments replace or upgrade these structures. This paper will examine four case studies of different collisions that have similar circumstances to the Francis Scott Key Bridge collapse and review National Transportation Safety Board reports …

Fatigue And Healing Performance Of Asphalt Materials Modified With Nanomaterials And High-Polymer Asphalt Binders, Samuel M. Addai

Theses and Dissertations

Fatigue cracking is one of the primary distress mechanisms affecting asphalt pavement durability under repeated traffic loading and environmental aging. Although asphalt binders possess an inherent ability to heal micro-damage during rest periods, this healing capacity is often insufficient to fully counter progressive fatigue damage under field conditions. This thesis investigates the fatigue and self-healing behavior of asphalt binders and mixtures modified with nanomaterials and advanced polymer-based systems. Nanomodified binders were evaluated using Linear Amplitude Sweep (LAS) and Linear Amplitude Sweep with Healing (LASH) tests within the VECD framework to quantify fatigue resistance and healing behavior under different rest periods …

Transport Properties And Microstructural Development Of Co₂-Cured, Co₂-Infused, And Cold-Weather Concrete, Arlene Jane Looc

Theses and Dissertations

This research aims to advance the understanding of durability and transport behavior in emerging CO₂-cured and cold-weather concrete technologies. Limited work has examined long-term durability indicators such as permeability, resistivity, and pore connectivity in these types of concrete. To bridge this gap, the study evaluates transport properties alongside microstructural changes that govern the progression of hydration and the evolution of pore structure. This study measures transport properties, determines correlations between resistivity and fluid ingress, and analyzes the evolution of pore structure. To achieve this, the experimental program evaluated three concrete mixtures for external CO₂ exposure, nine for dry ice infusion, …

Mental Health Self-Perceptions And Resource Utilization In Undergraduate Students At Binghamton University, Nicole J. Dreznin, Sarah Marcus

Binghamton University Undergraduate Journal

Undergraduate students face increasing rates of mental health challenges, however discrepancies in mental health literacy and utilization of resources may exist across student populations. This study examined the perceptions of mental health in undergraduate students at Binghamton University and if they differed among STEM vs non-STEM, man vs. women, and first-generation vs. continuing generation undergraduate students. A 20-question survey, containing 5 demographic questions and 15 mental health related questions was distributed through Qualtrics to 9 undergraduate classes. The available response choices were based on the Likert scale. A total of 1266 students participated.  Chi square tests of independence were done …

Vibro-Seismic Approach For Remediation Of Near-Wellbore Damage, Mounira Ahmed Abdallah

Theses and Dissertations

The research highlights the importance of vibro-seismic technology in the oil and gas industry. The primary objective of this study is to demonstrate how the vibro-seismic method enhances formation properties which are porosity, and permeability. Ultimately, this will lead to improved productivity and its application to remove the wellbore damage.

The research discusses whether or not the formation properties will be enhanced by the optimized acoustic vibration formation parameters. The thesis details laboratory experiments on core plugs, and four key findings are presented. The experimental work first showed that the use of the proper and optimum parameters enhanced the porosity …

Purification And Concentration Of Model Viruses Using Single-Pass Tangential Flow Filtration, Luis Alberto Mejía-Manzano, Lynn M. Manchester, Grace Wallis, Kan Wang, Bruce Lee, Michael Betenbaugh, Caryn Heldt

Michigan Tech Publications

The vaccine and viral vector industry is growing at an accelerated rate. To improve harvest and purification processes, the development of continuous membrane-based operations, such as normal flow filtration (NFF) and single pass tangential flow filtration (SPTFF) for concentration were explored. This work was conducted using two model viruses, non-enveloped porcine parvovirus (PPV) and enveloped Suid herpesvirus (SuHV). The viruses are in the same family as the gene therapy vectors adeno associated virus and herpes simplex virus, respectively. SPTFF design started with batch TFF for membrane selection. Hollow fiber membranes with a 100 and 300 kDa molecular weight cut off …