Engineering Commons^™

Effect Of Twisted Fiber Anisotropy In Cardiac Tissue On Ablation With Pulsed Electric Fields, Fei Xie, Christian W. Zemlin

Bioelectrics Publications

Background: Ablation of cardiac tissue with pulsed electric fields is a promising alternative to current thermal ablation methods, and it critically depends on the electric field distribution in the heart.

Methods: We developed a model that incorporates the twisted anisotropy of cardiac tissue and computed the electric field distribution in the tissue. We also performed experiments in rabbit ventricles to validate our model. We find that the model agrees well with the experimentally determined ablation volume if we assume that all tissue that is exposed to a field greater than 3 kV/cm is ablated. In our numerical analysis, we considered …

Cell Electrosensitization Exists Only In Certain Electroporation Buffers, Janja Dermol, Olga N. Pakhomova, Andrei G. Pakhomov, Damijan Miklavčič

Bioelectrics Publications

Electroporation-induced cell sensitization was described as the occurrence of a delayed hypersensitivity to electric pulses caused by pretreating cells with electric pulses. It was achieved by increasing the duration of the electroporation treatment at the same cumulative energy input. It could be exploited in electroporation-based treatments such as electrochemotherapy and tissue ablation with irreversible electroporation. The mechanisms responsible for cell sensitization, however, have not yet been identified. We investigated cell sensitization dynamics in five different electroporation buffers. We split a pulse train into two trains varying the delay between them and measured the propidium uptake by fluorescence microscopy. By fitting …

Cytosolic Dna Sensor Upregulation Accompanies Dna Electrotransfer In B16.F10 Melanoma Cells, Katarina Znidar, Masa Bosnjak, Maja Cemazar, Loree C. Heller

Bioelectrics Publications

In several preclinical tumor models, antitumor effects occur after intratumoral electroporation, also known as electrotransfer, of plasmid DNA devoid of a therapeutic gene. In mouse melanomas, these effects are preceded by significant elevation of several proinflammatory cytokines. These observations implicate the binding and activation of intracellular DNA-specific pattern recognition receptors or DNA sensors in response to DNA electrotransfer. In tumors, IFN β mRNA and protein levels significantly increased. The mRNAs of several DNA sensors were detected, and DAI, DDX60, and p204 tended to be upregulated. These effects were accompanied with reduced tumor growth and increased tumor necrosis. In B16. F10 …

Analysis Of Ca²⁺ Mobilization Of Nanosecond Pulses In Cells, Iurii Semenov

Bioelectrics Publications

Exposure to nanosecond electric pulses (nsEPs) evokes transient rise of cytosolic free Ca²⁺ concentration ([Ca²⁺]i) in the cells. We quantified the external Ca²⁺ uptake, intracellular Ca²⁺ release and uncover interplay of these processes for CHO-K1 cells upon exposure to single 60 ns pulse in the range of amplitudes from 0 to 30 kV/cm. NsEPs are distinguished by the ability to electroporate intracellular membranes. We studied how duration of nsEPs is related to efficiency of intracellular membranes electroporation comparing ability of single 300-, 60- and 10 ns pulses to recruit Ca²⁺ from endoplasmic reticulum (ER) in respect to the uptake of …

Electrosensitization Assists Cell Ablation By Nanosecond Pulsed Electric Field In 3d Cultures, Claudia Muratori, Andrei G. Pakhomov, Shu Xiao, Olga N. Pakhomova

Bioelectrics Publications

Previous studies reported a delayed increase of sensitivity to electroporation (termed "electrosensitization") in mammalian cells that had been subjected to electroporation. Electrosensitization facilitated membrane permeabilization and reduced survival in cell suspensions when the electric pulse treatments were split in fractions. The present study was aimed to visualize the effect of sensitization and establish its utility for cell ablation. We used KLN 205 squamous carcinoma cells embedded in an agarose gel and cell spheroids in Matrigel. A local ablation was created by a train of 200 to 600 of 300-ns pulses (50 Hz, 300-600 V) delivered by a two-needle probe with …

Activation Of Dna Pattern Recognition Receptors After Plasmid Electrotransfer In Melanoma Cells And Tumors, Loree Heller, Masa Bosnjak, Katarina Znidar, Maja Cemazar

Bioelectrics Publications

(First sentence) In vivo electroporation or electrotransfer, the application of controlled electric pulses, enhances delivery of plasmid DNA to a wide variety of healthy tissues as well as tumor types.

A Dielectric Rod Antenna For Picosecond Pulse Stimulation Of Neurological Tissue, Ross A. Petrella, Karl H. Schoenbach, Shu Xiao

Bioelectrics Publications

A dielectrically loaded wideband rod antenna has been studied as a pulse delivery system to subcutaneous tissues. Simulation results applying 100-ps electrical pulse show that it allows us to generate a critical electric field for biological effects, such as brain stimulation, in the range of several centimeters. In order to reach the critical electric field for biological effects, which is similar to 20 kV/cm, at a depth of 2 cm, the input voltage needs to be 175 kV. The electric field spot size in the brain at this position is similar to 1 cm(2). Experimental studies in free space with …

Comparison Between Electropositive And Electronegative Cold Atmospheric-Pressure Plasmas: A Modelling Study, Ding X. Liu, Jia F. Li, Ai J. Yang, Xiao H. Wang, Ming Z. Rong, Michael G. Kong

Bioelectrics Publications

Cold atmospheric-pressure He + N₂ and He + O₂ plasmas are chosen as the representatives for electropositive and electronegative plasmas, of which the discharge characteristics are studied and then compared to each other by fluid models. As the increase of the impurity (N₂ or O₂) fraction from 0 to 10%, for He + N₂ plasmas the electron density and ion density increase, the spatiotemporal distributions of electron density, ion density, electron temperature and electron generation rate change a little. On contrast, for He + O₂ plasmas the electron density decreases, the ion density …

Laboratory Exercise- Mapping The Risk Of Structural Failure In Schools Due To An Earthquake, Joel Barker, Kimberly Sowards, Casey Webb

Engineering Applications of GIS - Laboratory Exercises

Utah’s present geology has been greatly dictated by tectonic stresses that have occurred over the last 0.7 Ga. A determining factor in much of this Geology is the Hingeline that runs through Salt Lake County. Throughout the history of the western North America, this has been an important weak zone. It is no surprise that this same zone is now home to the Wasatch Fault System, which accommodates some of the extension of Western North America. The steep mountains facing west toward Salt Lake City were formed from the tectonic processes of the Fault. The Wasatch Fault is a normal …

Laboratory Exercise X – Most Dangerous Places To Live In The United States Based On Natural Disasters, Joshua Alpers, Luke Kevan, Santiago Tovar

Engineering Applications of GIS - Laboratory Exercises

This laboratory assignment simulates the most dangerous places to live in the United States based on natural disasters. The natural disasters that will be used in this assignment include wild fires, earthquakes, landslides, hurricanes, tornadoes, and flooding. These inputs will be required in creating a heat map of the United States showing what locations are most likely to have a high probability of natural disasters.

Laboratory Exercise – Ideal Locations For A Family, Benjamin Barrett, Colin Esplin, Chris Garcia

Engineering Applications of GIS - Laboratory Exercises

Upon the successful completion of a college degree, many students (and their families) are offered job positions in various locations throughout the Unites States. As a result, these young families are tasked with the challenge of finding a suitable place to live in an area they are completely unfamiliar with. By using a model in ArcMap this task can be made much easier. In this lab a model will be created in ArcGIS Model Builder along with a corresponding map showing suitable locations.

Final Laboratory Project – Emergency Landing Site Map, John Chadwick, Daniel Pope, Todd Weichers

Engineering Applications of GIS - Laboratory Exercises

Utah County is constantly developing and expanding. New developments require new roads, new power lines, and changes to existing topography. GIS data is maintained and made available for the state of Utah by Utah AGRC (Automated Geographic Reference Center). GIS can be used to make an easy to update map that will display the current situation of roads, power lines, population density, and terrain. In this exercise a model will be created that will execute to create a map that will display safe emergency landing sites for recreational aircraft in Utah County.

Laboratory Exercise – Determining Evacuation Route For Deer Creek Dam Failure, Eleise Hinton, Matt Randall, Brian Packer

Engineering Applications of GIS - Laboratory Exercises

Deer Creek Reservoir holds a volume of approximately 153,000 acre-ft of water. All of this water is held back by Deer Creek dam. Below the dam, the Provo River flows through Provo Canyon and down into Provo before terminating at Utah Lake. While the chance of a dam break is considered unlikely, an extreme event such as a major earthquake could trigger a break. In the case of a dam break, residents of Provo who live in the flood path will need to know where to evacuate. This exercise will create a model to show the nearest evacuation sites for …

Laboratory Exercise — Safety On Roads, Elise Estep, Kyungmin Kim, Danielle Nixon

Engineering Applications of GIS - Laboratory Exercises

In transportation study area, safety is a great issue. GIS is appropriate to perform spatial analysis the accident history in define area. Specifically, GIS allow researchers to find an area or road segment that have high probability of any type of car accident such as Vehicle to Vehicle, Vehicle to Pedestrian, and Vehicle to Bicycle.

Final Laboratory Project - Finding The Ideal Camping Location In The Uintas, Jeffrey Baker, Josh Curtis, Ryan Egbert

Engineering Applications of GIS - Laboratory Exercises

GIS is used extensively by the United States Forest Service in mapping and research. Many resources are available online including raster data and shapefiles. The use of GIS tools allows for analyzing entire forests and finding specific points within large areas that meet certain criteria. The Ashley National Forest, High Uinta Mountains Wilderness, in northeastern Utah contains many shapefiles including roads, forest, highway, points of interest, lakes, streams, etc. The purpose of this lab is to use these available datasets to identify ideal locations to set up camp in the Uinta Mountains.

Child Safety Near Pot Shops In Denver, Jacob Flansberg, Elise Jackson, Amelia Theobald

Engineering Applications of GIS - Laboratory Exercises

Some major factors in deciding where one’s family should live include relative safety of the neighborhood, available green space such as parks and schools, grocery stores, and other commercial retail in the area. With the legalization of marijuana in states such as Colorado, proximity of these marijuana dispensaries has become another factor. The Normalized Difference Vegetation Index (NDVI) can be used as a measure of vegetation, and to determine areas with high levels of irrigated vegetation. Using ArcGIS Modelbuilder, the data from an NDVI analysis can be used to determine the proximity of marijuana shops to these types of greenspaces …

Low Temperature Solution-Processed Sb:Sno2 Nanocrystals For Efficient Planar Perovskite Solar Cells, Yang Bai, Yanjun Fang, Yehao Deng, Qi Wang, Jingjing Zhao, Xiaopeng Zheng, Yang Zhang, Jinsong Huang

Department of Mechanical and Materials Engineering: Faculty Publications

Inorganic metal oxide electron-transport layers (ETLs) have the potential to yield perovskite solar cells with improved stability, but generally need high temperature to form conductive and defect-less forms, which is not compatible with the fabrication of flexible and tandem solar cells. Here, we demonstrate a facile strategy for developing efficient inorganic ETLs by doping SnO₂ nanocrystals (NCs) with a small amount of Sb using a low-temperature solution-processed method. The electrical conductivity was remarkably enhanced by Sb-doping, which increased the carrier concentration in Sb:SnO₂ NCs. Moreover, the upward shift of the Fermi level owing to doping results in improved …

Propagation Of Extensional Waves In A Piezoelectric Semiconductor Rod, C.L. Zhang, X. Y. Wang, W. Q. Chen, J. S. Yang

Department of Mechanical and Materials Engineering: Faculty Publications

We studied the propagation of extensional waves in a thin piezoelectric semiconductor rod of ZnO whose c-axis is along the axis of the rod. The macroscopic theory of piezoelectric semiconductors was used which consists of the coupled equations of piezoelectricity and the conservation of charge. The problem is nonlinear because the drift current is the product of the unknown electric field and the unknown carrier density. A perturbation procedure was used which resulted in two one-way coupled linear problems of piezoelectricity and the conservation of charge, respectively. The acoustic wave and the accompanying electric field were obtained from the equations …

Kentucky Water Resources Research Institute Annual Technical Report Fy 2015, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Annual Technical Reports (USGS’s 104b Grant Program)

The 2015 Annual Technical Report for Kentucky consolidates reporting requirements for the Section 104(b) base grant award into a single document that includes: 1) a synopsis of each student research enhancement project that was conducted during the period, 2) citations for related publications, reports, and presentations, 3) a description of information transfer activities, 4) a summary of student support during the reporting period, and 5) notable awards and achievements during the year.

No funds were requested for general program administration activities. However, travel funds were provided to support the participation of the director and associate director in the annual meeting …

Functional Performance Differences Between The Genium And C-Leg Prosthetic Knees And Intact Knees, Michael J. Highsmith, Jason T. Kahle, Rebecca M. Miro, M. E. Cress, Derek J. Lura, William S. Quillen, Stephanie Carey, Rajiv V. Dubey, Larry J. Mengelkoch

Mechanical Engineering Faculty Publications

Microprocessor prosthetic knees (MPKs) have advanced technologically, offering new features to decrease impairment and activity limitations for persons with transfemoral amputation (TFA). The Genium knee is functionally untested, and functional differences between it and intact knees are unknown. This study sought to determine whether Genium use improves functional performance compared with the C-Leg. A randomized experimental crossover design was used, with a cross-section of five nonamputee controls for comparison to nor­mal. Twenty community-ambulating persons with TFA were trained and tested for accommodation with study components. All subjects (n = 25) were assessed using the Continuous-Scale Physical Functional Performance-10 (CS-PFP10) …

User Involvement In The Partner Program Of The Educational Social Network, Ilya V. Osipov, Alex A. Volinsky, Anna Y. Praskikova

Mechanical Engineering Faculty Publications

The paper describes experiments to attract active online system users to the partner program. The objective is to grow the number of users by involving existing system users in viral mechanics. Several examples of user motivation are given, along with the specific interface implementations and viral mechanics. Viral K-factor was used as the metrics for the resulting system growth assessment. Specific examples show both positive and negative outcomes. Growth of the target system parameters is discussed.

A Tool For Beamforming And Real-Time Link Budget Analysis In Aeronautical Communications Using Kinematics, M. Cenk Erturk, Yasin Aksan

Electrical Engineering Faculty Publications

Aeronautical Communication (AC) systems are likely to be a part of future tiered communication network structures. Therefore maintaining a robust AC link with a minimum power burden on the host platform has a critical importance. In this paper, we analyze the AC systems from a link budget analysis point of view and define the requirements for the parameters of link budget with an emphasize on antenna gains. First, we study the link budget analysis in an AC system. Then, we present a mathematical framework to provide an end-to-end link budget analysis utilizing the platform kinematics. Finally, we present the numerical …

Modeling Of Rheological Properties For Entangled Polymer Systems, Nilanjana Banerjee

Doctoral Dissertations

"The study of entangled polymer rheology both in the field of medicine and polymer processing has their major importance. Mechanical properties of biomolecules are studied in order to better understand cellular behavior. Similarly, industrial processing of polymers needs thorough understanding of rheology so as to improve process techniques. Work in this dissertation has been organized into three major sections. Firstly, numerical/analytical models are reviewed for describing rheological properties and mechanical behaviors of cytoskeleton. The cytoskeleton models are classified into categories according to the length scales of the phenomena of interest. The main principles and characteristics of each model are summarized …

Assessment Of Highway Condition Using Combined Geophysical Surveys, Abdallah Alhadi Dera

Doctoral Dissertations

"Four pavement sections were investigated using ground penetrating radar (GPR) and Ultrasonic Surface Wave (USW). The objective of this research was to compare the effectiveness of two non-destructive geophysical tools, GPR and the PSPA, in assessing the condition of the pavements, composed of different construction materials. The GPR data were acquired using a 1.5 GHz antenna along five traverses spaced at two ft. intervals approximately 1000 ft. long. On the other hand, the PSPA data were acquired at the stations spaced at 1000 ft. along the five GPR traverses. Core samples were collected at each site to constrain the interpretation …

Microwave Material Characterization Of Alkali-Silica Reaction (Asr) Gel In Cementitious Materials, Ashkan Hashemi

Doctoral Dissertations

"Since alkali-silica reaction (ASR) was recognized as a durability challenge in cement-based materials over 70 years ago, numerous methods have been utilized to prevent, detect, and mitigate this issue. However, quantifying the amount of produced ASR byproducts (i.e., ASR gel) in-service is still of great interest in the infrastructure industry. The overarching objective of this dissertation is to bring a new understanding to the fundamentals of ASR formation from a microwave dielectric property characterization point-of-view, and more importantly, to investigate the potential for devising a microwave nondestructive testing approach for ASR gel detection and evaluation. To this end, a comprehensive …

Design, Fabrication, Operation And Aspen Simulation Of Oil Shale Pyrolysis And Biomass Gasification Process Using A Moving Bed Downdraft Reactor, Hassan Golpour

Doctoral Dissertations

"Energy is the major facilitator of the modern life. Every developed and developing economy requires access to advanced sources of energy to support its growth and prosperity. Declining worldwide crude oil reserves and increasing energy needs has focused attention on developing existing unconventional fossil fuels like oil shale and renewable resources such as biomass. Sustainable, renewable and reliable resources of domestically produced biomass comparing to wind and solar energy is a sensible motivation to establish a small-scale power plant using biomass as feed to supply electricity demand and heat for rural development. The work in Paper I focuses on the …

Novel Approaches To Clustering, Biclustering Algorithms Based On Adaptive Resonance Theory And Intelligent Control, Sejun Kim

Doctoral Dissertations

"The problem of clustering is one of the most widely studied area in data mining and machine learning. Adaptive resonance theory (ART), an unsupervised learning clustering algorithm, is a clustering method that can learn arbitrary input patterns in a stable, fast and self-organizing way. This dissertation focuses on unsupervised learning methods, mostly based on variations of ART.

Hierarchical ART clustering is studied by generating a tree of ART units with GPU based parallelization to provide fast and finesse clustering. Experiment results show that the our method achieves significant training speed increase in generating deep ART trees compared with that from …

Synthesis And Functionalization Of A Triaryldiamine-Base Photoconductive/Photorefractive Composite, And Its Application To Aberrated Image Restoration, Yichen Liang

Doctoral Dissertations

"Organic photorefractive (PR) composites have recently emerged as an important class of materials for applications including high-density data storage, optical communication, and biomedical imaging. In an effort to further improve their performance, this study focused on the utilization of functionalized semiconductor nanocrystals to photosensitize triaryamine (TPD)-based PR composites, as well as the application of TPD-based PR composites in the restoration of aberrated optical information. A novel approach to functionalize CdSe quantum dot (QCdSe) was firstly introduced where the sulfonated triarydiamine (STPD) was used as charge-transporting ligand to passivate QCdSe. TPD-based photoconductive and PR composites were photosensitized with the STPD-passivated QCdSe …

Adaptive Filters For Sparse System Identification, Jianming Liu

Doctoral Dissertations

"Sparse system identification has attracted much attention in the field of adaptive algorithms, and the adaptive filters for sparse system identification are studied. Firstly, a new family of proportionate normalized least mean square (PNLMS) adaptive algorithms that improve the performance of identifying block-sparse systems is proposed. The main proposed algorithm, called block-sparse PNLMS (BS-PNLMS), is based on the optimization of a mixed ℓ_2,1 norm of the adaptive filter's coefficients. A block-sparse improved PNLMS (BS-IPNLMS) is also derived for both sparse and dispersive impulse responses. Meanwhile, the proposed block-sparse proportionate idea has been extended to both the proportionate affine projection …

Geophysical Imaging Of Karst Features In Missouri, Jeremiah Chukwunonso Obi

Doctoral Dissertations

"Automated electrical resistivity tomography (ERT) supported with multichannel analysis of surface waves (MASW) and boring data were used to map karst related features in Missouri in order to understand karst processes better in Missouri. Previous works on karst in Missouri were mostly surficial mapping of bedrock outcrops and joints, which are not enough to define the internal structure of karst system, since most critical processes in karst occur underground. To understand these processes better, the density, placement and pattern of karst related features like solution-widened joints and voids, as well as top of bedrock were mapped. In the course of …