Engineering Commons^™

Exploring Biomarkers For Point Of Care Bladder Cancer Detection, Cassandra M. Walker

Biomedical Engineering Undergraduate Honors Theses

Bladder cancer is the 5th most common non-cutaneous human cancer in the United States. While effective methods of detecting bladder cancer are currently practiced, they are often expensive and invasive. There is a need for a noninvasive detection method that can be used in areas with few medical resources. Cell free DNA in urine is normally present only in very low concentrations. Abnormally high levels of cell free DNA in urine could be indicative of disease. This study tests the hypothesis that DNA present in urine can be used as a biomarker for bladder cancer before hematuria is seen in …

Optimized Protocol For Measuring 2-Nbdg Uptake As A Cellular Marker Of Glycolytic Demand, Drew Folgmann

Biomedical Engineering Undergraduate Honors Theses

2[N-(7-nitrobenz-2-oxa-1,2-diaxol-4-yl)amino]-2-deoxyglucose (2-NBDG) a fluorescently tagged analog of FDG is ideal for in vitro studies and imaging. 2-NBDG has been proven to be an adequate marker for glucose uptake in many different types of cells [1]. However, across the spectrum of 2-NBDG use a lack of consensus is observed for the following questions. What is the ideal time to fast cells to optimize cellular uptake of 2-NBDG? Also, what is the ideal concentration of 2-NBDG to be used when quantifying glucose uptake? Finally, what is the effect of serum on the uptake of 2-NBDG? To answer these questions and understand glucose …

Micellular Electrokinetic Chromatography For Studying Amyloid Beta Oligomer Membrane Affinity, Andrew Bryson

Biomedical Engineering Undergraduate Honors Theses

Amyloid Beta (Aβ) was the major focus of this study. It is a peptide that is present in the brain with a high tendency to self-aggregate. When this protein aggregates, it forms oligomers and protofibrils which in turn are deposited as senile plaques in the brain. The reason for the concern with these plaques is their association with the neurological disorder Alzheimer’s disease. It has been found that the most dangerous oligomers are formed in a portion of the plasma membrane known as lipid rafts. The purpose of this study was to understand how micelles affect the aggregation properties of …

Non-Directional Conjugation Of Fluorescent Antibodies To Gold Nanoparticles For Stem Cell Therapy, Kunal B. Shah

Biomedical Engineering Undergraduate Honors Theses

The objective of this study was to design citrate-coated gold nanoparticles conjugated with FITC-IgG, a fluorescent antibody, and to qualitatively and quantitatively measure the resulting fluorescent emission. Optical properties of the gold nanoparticles were measured at various stages to provide evidence of successful conjugation. The absorbance spectrum of the citrate gold nanoparticles was compared to that of the reaction mixture containing the gold nanoparticles and the FITC-IgG. A noticeable broadening of the absorption peak was observed at 519 nm indicating a surface modification of the gold nanoparticles. Fluorescence data was obtained with a fluorospectrometer and revealed a significant amount of …

Design Of A 3d-Printed Device For Diffuse Reflectance Spectroscopy Calibration As A Step Towards Clinical Translation, Nontapoth Vongkittiargorn

Biomedical Engineering Undergraduate Honors Theses

The diffusive reflectance and spectroscopic microendoscopy (DRSME) is a multimodal imaging system that harnesses its usefulness from different light sources. One of the modalities, diffuse reflectance spectroscopy (DRS), has been used in our lab to investigate optical properties of epithelial tissues using a broadband white light as the main source. Calibration of DRS is required as it can obtain the maximum intensity and convert it to absolute reflectance. Current manual-adjusted calibration can be lengthy and often lead to inconsistent results. Therefore, a new method of calibration is introduced where additive manufacturing (or 3D printing) technology is fully utilized.

The instrumentation …

Design And Resistance Factor Calibration Of Pipe Piles In California, Yujie Hu

Civil Engineering Theses - Archive

Steel pipe piles, either open ended or close ended, are widely used in the state of California as bridge foundations due to high load bearing capacity, light weight, and outstanding workability. The design methods currently used by Caltrans were developed based on limited field data and are lack of field validations of the recent development of steel pipe piles. In this thesis study, 45 high quality steel pipe pile load tests performed in various soil conditions were collected from Caltrans’ load test data to evaluate available design methods and calibrate the resistance factors of selected design methods. A total of …

Potential Application Of Ultra-High Performance Fiber-Reinforced Concrete With Wet-Mix Shotcrete System In Tunneling, Jean Gamarra

Civil Engineering Theses - Archive

In the tunneling industry, shotcrete has been used for several decades. The use of shotcrete or wet-mix spray-on methods allows the application of this method in complex underground profiles and shapes. The need for time efficient spraying methods and constructability for lining coverage opens the door for technologies like steel and synthetic fiber reinforced shotcrete to achieve a uniform and a good quality product. An important advantage of the application of fiber reinforced concrete in shotcrete systems for tunneling is that almost no steel fixing is required. This leads to several other advantages including safer working conditions during excavation, less …

Usb-Powered Turntable "Turn T", Gerardo Sevilla, James Lougaris

Electrical Engineering

No abstract provided.

Multiphase Ferrofluid Flows For Micro-Particle Focusing And Separation, R. Zhou, Cheng Wang

Mechanical and Aerospace Engineering Faculty Research & Creative Works

No abstract provided.

Strong Coupling Between Mid-Infrared Localized Plasmons And Phonons, W. Wan, Xiaodong Yang, Jie Gao

Mechanical and Aerospace Engineering Faculty Research & Creative Works

No abstract provided.

The Child Brain Computes And Utilizes Internalized Maternal Choices, S. Seung-Lark Lim, J. Cherry C. Bradley, A. Davis, S. N. Balakrishnan, O. Ha, J. Bruce, A. Bruce

Mechanical and Aerospace Engineering Faculty Research & Creative Works

No abstract provided.

Rapid, Low-Cost Biosensor For Water-Borne Pathogens In Low-Resource Environments, Kimberly Cribbs

Chemical Engineering Undergraduate Honors Theses

When testing water quality, “microbiological results typically require a minimum of 24 hours to complete” due to reliance on the traditional and inexpensive cultivation method. In 2000, an isothermal DNA amplification method termed loop-mediated isothermal amplification (LAMP) was invented allowing for microbiological assessments to be performed in less than one hour without the need for an expensive thermocycler. In 2013, the World Health Organization reported LAMP as an “attractive diagnostic platform for resource-poor settings: it is fast [15-40 min], isothermal [requiring only a heat block], robust to inhibitors and reaction conditions that usually adversely affect PCR methods, and it generates …

Assessing The Limitations And Capabilities Of Lidar And Landsat 8 To Estimate The Aboveground Vegetation Biomass And Cover In A Rangeland Ecosystem Using A Machine Learning Algorithm, Shital Dhakal

Boise State University Theses and Dissertations

Remote sensing based quantification of semiarid rangeland vegetation provides the large scale observations required for monitoring native plant distribution, estimating fuel loads, modeling climate and hydrological dynamics, and measuring carbon storage. Fine scale 3-dimensional vertical structural information from airborne lidar and improved signal to noise ratio and radiometric resolution of recent satellite imagery provide opportunities for refined measurements of vegetation structure.

In this study, we leverage a large number of time series Landsat 8 vegetation indices and lidar point cloud - based vegetation metrics with ground validation for scaling aboveground shrub and herb biomass and cover from small scale plot …

Impedance Spectroscopy Studies Of Yttria Stabilized Zirconia Under Extreme Conditions, Quinlan Blaine Smith

UNLV Theses, Dissertations, Professional Papers, and Capstones

Yttria Stabilized Zirconia (YSZ) is of interest for many industries. Varying amounts of Yttria (Y2O3) can be doped into Zirconia (ZrO2) to create materials with specific characteristics. For instance, 3mol% YSZ (3YSZ) is known to be a super hard material and is used as a coating on drill tips and as an abrasive. Eight mol% YSZ (8YSZ) is commonly used as a solid electrolyte in Solid Oxide Fuel Cells because of its good ionic conducting abilities and stability at high temperatures. In this thesis project, a novel experimental setup was created and used to study the ionic conductivity of (3 …

Minimizing Ethanol Concentration In Organosolv Pretreatment For The Saccharification Of Loblolly Pine, Nelson B. Heringer

Biological and Agricultural Engineering Undergraduate Honors Theses

Organic solvent pretreatment, commonly known as organosolv, is a method used to prepare biomass for enzymatic hydrolysis for the production of biofuels. This method common uses ethanol as the organic solvent. However, this creates an economic issue with the product stream, as ethanol becomes a product and input. This project sought to explore how decreasing the use of ethanol in organosolv pretreatment affected the recoverability of sugars after enzymatic hydrolysis. Loblolly pine (Pinus taeda L.) was pretreated at 170 °C for 60 minutes in 1% dilute sulfuric acid and an ethanol concentration varying from 65% to 35%. Compositional …

Municipal Composting And Organic Waste Diversion: The Case Of Fayetteville, Arkansas, Michael E. Hoppe

Biological and Agricultural Engineering Undergraduate Honors Theses

It is estimated that 40% of food is wasted in the United States; representing $165 billion in wasted resources. A vast majority of that wasted food is ultimately placed in landfills where it decomposes and releases harmful greenhouse gases (GHGs). In fact, food waste alone is responsible for 23% of annual methane emissions for the US. This has a huge impact on global climate change due to the potency of methane as a greenhouse gas. Currently only 5% of the food waste produced is recovered across the nation. Source reduction would be the best solution to reducing this food waste, …

Strengthening Of Lightweight Reinforced Concrete Beams Using Carbon Fiber Reinforced Polymers (Cfrp), Tariq Al Jaafreh

Civil Engineering Dissertations - Archive

This research aimed to investigate the effect of utilizing “Fiber Reinforced Polymer” (FRP) on the light weight concrete beams. The main hypothesis is that such utilization of the FRP will lead to strengthened lightweight beams. The research that has been done on this relationship is somewhat broad in the scope and have mostly focused on the normal-weight concrete. However, only few studies that have considered the light weight concrete. Eight Beams were tested in this research under four point bending test. Two beams were taken as a control beams without any external bonding of Carbon Fiber Reinforced polymer. Another two …

Gold Nanoparticle Colorimetric Sensing Technology, Eve Borden

Nanoscale Science & Engineering (discontinued with class year 2014)

No abstract provided.

Size Controlled Formation Of A Collagen-Based Drug Delivery System, Leonardo Bezerra

Nanoscale Science & Engineering (discontinued with class year 2014)

This study investigates and troubleshoots various factors involved in the generation of collagen-based particles, such as volume of solution, temperature, and incubation time for the purposes of a potential colorectal cancer targeting drug vehicle. The particles were generated via loading into a gel mold and show a 20-200 fold decrease in size compared to those created only under chemical means.

Fundamental Interdiffusion Analysis Of Ruthenium And Cobalt Films, Bartlet Deprospo

Nanoscale Science & Engineering (discontinued with class year 2014)

No abstract provided.

Graphene’S Electronic Surface States On Metal Substrates, Alex De Palma

Nanoscale Science & Engineering (discontinued with class year 2014)

Graphene is a 2-­‐D sheet of sp2 bonded carbon atoms with exceptional electrical properties. Particularly, graphene has a very high carrier mobility (~200,000 cm2/V·∙s). This is largely due to graphene’s unique electronic structure, wherein charge carriers are effectively massless Dirac fermions. However, the unique electronic structure of graphene has been shown to be affected by the underlying substrate. In this study, we characterize the electronic structure of graphene on copper. Electron energy loss spectroscopy is employed to observe the surface plasmon excitations of graphene. A small pi plasmon excitation is observed, suggesting that the graphene/Cu interaction is weak and non-­‐covalent. …

Modeling Secondary Electron Trajectories In Scanning Electron Microscopes, Kevin Mcnamara, Joshua Miller

Nanoscale Science & Engineering (discontinued with class year 2014)

The efficiency of secondary electron collection by a scanning electron microscope detector is not generally known, particularly as the electric field on the detector is varied. It is often assumed that the detector collects almost all of the secondary electrons emitted from the sample. This works seeks to better understand the mechanism of secondary electron collection by the detector in order to optimize collection efficiency. The benefit of collecting more secondary electrons is the enhancement of the signal-to-noise ratio, which means better quality images can be obtained, allowing us to better understand the relationship between secondary electron images and the …

Modeling Secondary Electron Trajectories In Scanning Electron Microscopes, Joshua Miller, Kevin Mcnamara

Nanoscale Science & Engineering (discontinued with class year 2014)

The efficiency of secondary electron collection by a scanning electron microscope detector is not generally known, particularly as the electric field on the detector is varied. It is often assumed that the detector collects almost all of the secondary electrons emitted from the sample. This works seeks to better understand the mechanism of secondary electron collection by the detector in order to optimize collection efficiency. The benefit of collecting more secondary electrons is the enhancement of the signal-to-noise ratio, which means better quality images can be obtained, allowing us to better understand the relationship between secondary electron images and the …

Review And Experimental Investigation Of Phase Change Material Use In Buildings, Russell W. Locetta

Mechanical Engineering Undergraduate Honors Theses

This paper presents an overview of phase change materials (PCM) and their properties along with a review of relevant studies conducted in the past several years over phase change materials. An experiment is conducted to examine the differences between external building walls with and without bio-based phase change materials. In addition, the length of time BioPCM requires to completely change phase is examined from the data. Based on the data, the internal wall temperature of a home can be reduced by approximately 2 F. Additionally, a theoretical case study is presented and it is concluded that with the use of …

Droplet Generation At Megahertz Frequencies, John C. Miers

Mechanical Engineering Undergraduate Honors Theses

Droplet formation has been a fascinating subject to scientists for centuries due to its natural beauty and importance to both scientific and industrial applications, such as inkjet printing, reagent deposition, and spray cooling. However, the droplet generation frequency of common drop-on-demand jetting techniques is mostly limited to ~10 kHz. This paper presents an investigation of the possibility of jetting at megahertz frequencies in order to boost the productivity of drop-on-demand material deposition by ~100 times. The focus of this paper is to understand the limitations of generating droplets at a megahertz frequency and to explore possible solutions for overcoming these …

Finite Element Modeling Of Shallowly Embedded Connections To Characterize Rotational Stiffness, Trevor Alexander Jones

Theses and Dissertations

Finite element models were created in Abaqus 6.14 to characterize the rotational stiffness of shallowly embedded column-foundation connections. Scripts were programmed to automate the model generation process and allow study of multiple independent variables, including embedment length, column size, baseplate geometry, concrete modulus, column orientation, cantilever height, and applied axial load. Three different connection types were investigated: a tied or one part model; a contact-based model; and a cohesive-zone based model. Cohesive-zone modeling was found to give the most accurate results. Agreement with previous experimental data was obtained to within 27%. Baseplate geometry was found to affect connection stiffness significantly, …

Automatic Dependent Surveillance-Broadcast For Detect And Avoid On Small Unmanned Aircraft, Matthew Owen Duffield

Theses and Dissertations

Small unmanned aircraft systems (UAS) are rapidly gaining popularity. As the excitement surrounding small UAS has grown, the Federal Aviation Administration (FAA) has repeatedly stated that UAS must be capable of detecting and avoiding manned and unmanned aircraft. In developing detect-and-avoid (DAA) technology, one of the key challenges is identifying a suitable sensor. Automatic Dependent Surveillance-Broadcast (ADS-B) has gained much attention in both the research and consumer sectors as a promising solution. While ADS-B has many positive characteristics, further analysis is necessary to determine if it is suitable as a DAA sensor in environments with high-density small UAS operations. To …

Error Propagation Dynamics Of Piv-Based Pressure Field Calculation, Zhao Pan

Theses and Dissertations

Particle Image Velocimetry (PIV) based pressure field calculation is becoming increasingly popular in experimental fluid dynamics due to its non-intrusive nature. Errors propagated from PIV results to pressure field calculations are unavoidable, and in most cases, non-negligible. However, the specific dynamics of this error propagation process have not been unveiled. This dissertation examines both why and how errors in the experimental data are propagated to the pressure field by direct analysis of the pressure Poisson equation. Error in the pressure calculations are bounded with the error level of the experimental data. The error bounds quantitatively explain why and how many …

Electrical And Manufacturing Limitations For The Miniaturization Of Ion Trap Devices With Digital Excitation, Derek Joseph Andrews

Theses and Dissertations

Developing portable mass spectrometry systems is an active area of research due to its broad range of useful applications, including environmental monitoring, threat detection, and space exploration. The mass analyzer is one of the key elements of the mass spectrometry system to develop for a portable system. Ion traps are a good candidate for the mass analyzer in a portable mass spectrometry system because the operating pressure scales with size. This allows for scaling down the other components of the system including the vacuum and electrical systems. Researchers at BYU are making an effort to develop miniature ion traps based …

Comparison Of Construction Methods For Building An Underground Freight Transportation In Texas, Niloofar Rezaei

Civil Engineering Theses - Archive

Freight transportation by trucks constitutes approximately 80% of total freight transportation in Texas and it is expected to approximately double by the year 2040. Social and environmental costs are adverse impacts of moving freights by trucks. Underground Freight Transportation (UFT) significantly reduces these negative impacts and is more sustainable method of freight transportation. UFT includes close-fitting vehicles carrying freight through underground pipelines or tunnels between terminals. The main objective of this research is to compare two construction methods: (1) tunneling using tunnel boring machine (TBM) and (2) tunneling using open-cut. To conduct this research, a comprehensive literature search on various …