Engineering Commons^™

Cell-Free Protein Synthesis Reactor Formats: A Brief History And Analysis, Dallin M. Chipman, Anna C. Woolley, Davu N. Chau, William A. Lance, Joseph P. Talley, Tyler P. Green, Benjamin C. Robbins, Bradley Charles Bundy

Faculty Publications

Cell-free protein synthesis (CFPS) has transformed protein production capabilities by eliminating cellular constraints, enabling the rapid expression of difficult-to-produce proteins in an open, customizable environment. As CFPS applications expand from fundamental research to industrial production, therapeutic manufacturing, and point-of-care diagnostics, the diverse array of reactor formats has become increasingly important yet challenging to navigate. This review examines the evolution and characteristics of thirteen major CFPS reactor formats, from traditional batch systems to advanced platforms. The historical development of CFPS reactors from the 1960s to present day is presented. Additionally, for each format, operational principles, advantages, limitations, and notable applications are …

A Recycling Route For E-Waste And Aluminium Scrap To Produce A2xxx And A3xx Al Alloys, Iman El-Mahallawi, Shaza Raji, Mayar M. Ali, Mai Mahmoud, Sara Atef, Mahmoud Tash, Serag Salem, Tarek Elmeligy

Mechanical Engineering

Electronic waste (e-waste) generation is significantly escalating, while traditional recycling methods, failing to meet sustainability criteria, are still used. The implementation of sustainability requirements calling for recycling will not be achieved unless intensive research is conducted for mitigating and tolerating the presence of accumulating tramp elements in the recycled alloys and even revising the existing standard specifications. This study explores an innovative approach to recycling electronic waste (e-waste) by integrating it with aluminum scrap through conventional melting techniques to produce high-performance industrial alloys aiming to develop novel aluminum alloys with enhanced properties suitable for various industrial applications. The experimental process …

Evaluation Of Waste Plastic Addition Via Dry Method On Asphalt Mixture Performance, Isabella Madeira Bueno

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Incorporating waste plastic (WP) into hot mix asphalt (HMA) offers a promising approach to enhance recycling rates and improve asphalt concrete (AC) performance, particularly in moisture damage resistance. However, results vary widely due to differences in WP types, sizes, and mixing methods. The lack of standardized procedures, especially for the dry process, has led to inconsistent integration and unclear roles of WP in AC mixtures. Studies suggest that introducing WP to pre-heated aggregates at temperatures exceeding its melting point can enhance mixture resistance to rutting, cracking, and moisture damage. In this context, this study was conducted in two phases to …

Data-Driven Methods For Signal Analysis And Nonlinear System Identification, Cristian Felipe López Ruano

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

In recent years, data-driven modeling has gained considerable attention in nonlinear system identification, offering a powerful alternative to traditional physics-based methods. This has been possible by advances in machine learning, sparse regression, and differential equations in a weak form, which allow the accurate capture of complex nonlinear dynamics, even when the measurements are in strong background noise. Moreover, integrating these models with physical knowledge further enhances their reliability and interpretability, making them suitable for a wide range of science and engineering applications.

In this dissertation, we investigate algorithms for data-driven nonlinear system identification to obtain ordinary differential equations that govern …

Biocomputing Approach To Modeling And Modulating Calcium Signaling, Sehee Sun

School of Computing: Dissertations, Theses, and Student Research

Biocomputing is an emerging field that seeks to perform computational tasks using biological substrates and processes. Unlike conventional computing systems based on silicon hardware, biocomputing leverages the parallelism, energy efficiency, and complex dynamics of living systems. Among various cellular mechanisms, calcium (Ca2+) signaling stands out as a central regulator of diverse biological functions, offering a promising basis for programmable logic and control in living cells.

This thesis introduces a novel framework for modeling and modulating Ca2+ dynamics using biologically inspired Boolean logic circuits. Specifically, we propose the Ca2+ Boolean Logic (CaBL) model, in which Ca2+ fluxes and interactions are abstracted …

Elucidating The Impact Of Common Stormwater Pollutants On Antibiotic Resistance: The Role Of Heavy Metals, Nutrients, And Salts, Kassidy N. O'Malley, Patrick J. Mcnamara, Walter M. Mcdonald

Civil and Environmental Engineering Faculty Research and Publications

Antibiotic resistance poses a significant global health threat, and the urban water cycle presents an opportunity to augment or limit the spread of antibiotic resistance. In particular, stormwater runoff has recently been revealed as a key conduit for antibiotic-resistant bacteria (ARB). The specific role of stormwater pollutants, however, on antibiotic resistance has not been isolated. Understanding the impact of specific pollutants common to stormwater could help optimize design and operation of stormwater systems for management of antibiotic resistance. The objective of this research was to establish the potential contributions of common stormwater pollutants to antibiotic resistance proliferation. Lab-scale stormwater microcosms …

Novel Respirometry Measurement And Comparison Of Monod Kinetic Constants And Methanogen Concentrations For 27 Full-Scale Anaerobic Bioreactors, Nicholas Benn, Antonio Martins, Daniel H. Zitomer

Civil and Environmental Engineering Faculty Research and Publications

Monod kinetic coefficient values (km and Ks) as well as methanogen concentrations (Xac and Xh2) for various anaerobic bioreactors are required to gain a deeper understanding of anaerobic biotechnology. However, there are no existing, practical methods to measure or estimate these parameters or variables in typical anaerobic digester biomass that contains mixed cultures with inert organic solids. Current measurement methods rely on pure cultures developed in the laboratory. In this work, a novel respirometric method was developed and used to determine methanogen Monod parameters and active biomass concentration for samples from 27 full-scale anaerobic reactors. Differences (one to three orders …

Using Knovel Steam Calculators Instead Of Printed Steam Tables, Anthony Reynolds

Case studies: Digital Education

Applied Thermodynamics is a cornerstone module in mechanical engineering curricula, providing students with the foundational understanding of energy systems, heat transfer, and the behaviour of working fluids such as steam. Traditionally, students have relied on printed steam tables found in the appendix of class textbooks to determine thermodynamic properties—an approach that, while educational, can be both time-consuming and prone to errors. With the increasing availability of digital tools in education, there is a growing opportunity to enhance the teaching and learning of technical subjects through technology. One such tool is the Knovel Steam Calculators, an interactive, web-based resource that allows …

Exogenous Ketones Reduce Inflammation And Pathology In The Tgf344-Ad Rat Model, Amaya Rogers, Pheven Yohannes, Juan P. Carcamo, Preetham Gundlapally, Amaya Coker, Macy A. Seijo, Caesar M. Hernandez

Expo Student Presentations

2025 Summer Expo Poster Presentation

• Biological & Life Sciences
• Engineering
• Physical & Applied Sciences

A Uas-Centered Investigation Of Vorticity Characteristics And Cold Pool Structure Across Forward And Left-Flank Boundaries In Supercells, Mark R. De Bruin

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Supercell internal boundaries are the locus of tornadogenesis; thus, understanding the characteristics of these boundaries, particularly in terms of vorticity, is important for identifying the role they play in tornado formation. Insight into the overall characteristics of internal boundaries and their possible role in tornadogenesis have been driven by studies reliant on numerical modeling-based experiments. Observational studies often neglect above-surface conditions or, when these observations are made, lack the spatial resolution to resolve boundary characteristics. During TORUS (Targeted Observation by Radars and UAS of Supercells) 2019 and TORUS-LItE (TORUS Left-flank Intensive Experiment) 2023, uncrewed aircraft systems (UAS) and mobile mesonets …

Development And Implementation Of A Vector-Quantized Transformer Autoencoder For Monitoring Bicycle Road Conditions, Kewei Ren

Department of Construction Engineering and Management: Dissertations, Theses, and Student Research

Adviser: Chun-Hsing Ho

The long-term, sustainable assessment of bicycle road conditions is essential for ensuring cyclist safety and promoting equitable infrastructure management. While traditional detection methods based on manual thresholds have demonstrated some effectiveness, they carry limitations due to the diversity of pavement materials, cyclist behaviors, and sensor platforms. Although recent advances in deep learning offer promising alternatives, established techniques are primarily applied to highway anomaly detection and are rarely adapted for bicycle roads. Furthermore, most related studies lack clear definitions for anomalies and transparent annotation procedures, resulting in a shortage of effective datasets and detection methods specifically for bicycle …

Feasibility Of Using Recycled Waste Plastic In Concrete Pavements In Nebraska, Amasi Hajahja

Department of Construction Engineering and Management: Dissertations, Theses, and Student Research

Waste plastic (WP) is a growing environmental concern due to its non-biodegradable nature, large-scale production, and harmful impact on natural resources. With nearly 75% of WP in the United States ending up in landfills, effective recycling strategies are urgently needed. In parallel, the limited availability of natural aggregates highlights the need for alternative materials in concrete construction. This study investigates the feasibility of using recycled waste plastic (RWP) in concrete mixtures, both as aggregates and fibers for rigid pavement applications in Nebraska. Concrete mixtures were prepared by partially replacing natural fine aggregates with recycled plastic aggregates (RPA) at replacement levels …

Conservation Management Effects On Soil And Agronomic Properties In A Furrow-Irrigated Corn System In East-Central Arkansas, Jeferson P. Pimentel, Christopher G. Henry, Kris R. Brye, Benjamin R. Runkle, Amanda J. Ashworth, Edgar Mersiovsky, Kevin C. Thompson

Biological and Agricultural Engineering Faculty Publications and Presentations

Cover crops can improve soil physical properties and help to sustain agricultural crop yields. A 6-year study conducted from 2017 to 2022 examined the effects of conservation soil management practices in a furrow-irrigated continuous corn (Zea mays L.) system on a silt-loam soil (Typic Albaqualf) in the Lower Mississippi River Delta region of eastern Arkansas. This study compared the effects of conventional tillage (CT), no-tillage (NT), and no-tillage with cover crops (CC) in selected soil physical and chemical properties (i.e., soil bulk density (D b), porosity (& fnof;t), field capacity (Theta FC), Permanent wilting point (Theta PWP), Plant available water …

Aircraft Bird Strike Risk Prediction Using Machine Learning And Analytic Hierarchy Process, Jason Anthony Powell

Doctoral Dissertations and Master's Theses

To address the limitations of Next Generation Radar-based bird strike forecasting, this study modeled 12 spatiotemporal weather features from the National Oceanic and Atmospheric Administration alongside bird strike risk using Long Short-Term Memory Recurrent Neural Network (LSTM-RNN), XGBoost regression tree, and Bayesian network algorithms. Five years of bird strike data from four geographically diverse airfields served as the target risk variable, categorized as low, moderate, or severe based on Department of the Air Force risk models. The ensemble model, which combines the LSTM-RNN and XGBoost regression algorithms, yielded the most accurate forecasts, achieving 80% to 93% accuracy across all airfields, …

Statistical Monitoring Of Hard Faults In Digital Systems, Dany Akshay Deep Isukapalli

Electrical Engineering Theses and Dissertations

Achieving a high test coverage is crucial for helping to ensure that integrated circuits are working correctly and are non-defective. Although scan-based structural tests are used throughout the industry, high-level functional tests may be needed to detect some defects— especially those that are environmentally sensitive. Unfortunately, the character of functional test makes it difficult to obtain high coverage, and it is even hard to estimate coverage because fault simulation times of large circuits are long. As a result, some method is required for predicting the ability of a functional test that has not been fault simulated to detect defects. In …

Artificial Intelligence In Everyday Life, Sally Brown

Artificial Intelligence Exhibit

This section explores using AI in everyday life including decision making.

Artificial Intelligence: The Twilight Zone And Conclusion, Sally Brown

Artificial Intelligence Exhibit

This section concludes the exhibit with an exploration of the deepfake dilemma, existential risks and societal impacts, and takeaway inquiries.

Ai Questions; "The Ai Tea", Sally Brown

Artificial Intelligence Exhibit

This section includes a series of questions related to AI and the exhibit content, as well as button designs by WVU students and Art in the Libraries committee members, a list of the exhibition sponsors, and information on the exhibition launch panel.

Artificial Intelligence Introduction Section, Seth Newell, Sally Brown

Artificial Intelligence Exhibit

The introduction gives an overview of the exhibition, along with an explanation of AI literacy, AI vs. Google, and a basic AI timeline.

Artificial Intelligence In Education, Sally Brown, Jill Woods, Mohamed Hefeida, Jennifer Sano-Franchini, Megan Vendemia, Erin Brock Carlson, Megan Leight, Gangqing Hu, Nicole Fuller

Artificial Intelligence Exhibit

This section explores the intersection of AI and education, including an overview of WVU's AI statement and submissions from WVU faculty, classes, students, and staff on AI related projects.

Advancing Circular Bioeconomy Through A Systems-Level Assessment Of Food Waste And Industrial Sludge Codigestion, Md Nizam Uddin, Cassidy Hartog, Emma Murray, Jacob Loveless, Luke Roberson, Asli Aslan, Francisco Cubas, Stetson Rowles

Civil Engineering & Construction: Faculty Publications

Disposal of food waste (FW) in landfills remains an unsustainable practice for organic waste management. Simultaneously, pulp and paper mills produce significant amounts of recalcitrant organic waste that is difficult to decompose due to its high lignocellulosic content. In this study, we developed an innovative approach to improve the digestion of pulp and paper mill sludge (PPMS) by amending FW to produce a low chemical oxygen demand (COD) sludge while recovering methane in the process. This codigestion process was evaluated through lab-scale biogas production experiments coupled with a comprehensive economic and environmental sustainability assessment. Biomethane production results revealed that the …

Teaching Learning Newsletter Vol 5 Issue 1, Sastra Deemed To Be University

TLC Newsletter

No abstract provided.

Response Of Soybean To Variable Irrigation Levels In Eastern Nebraska Using The Aquacrop Model, Anmol Singh

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

AquaCrop was calibrated and validated for soybean [Glycine max (L.) Merr.] using 19 irrigation treatments from five years using data from field experiments. The model accurately simulated canopy cover (CC), soil water content (SWC), and grain yield, with overall validation nRMSE values of 12%, 7%, and 7%, respectively. The overall validation nRMSE for SWC in individual 30-cm soil layers was comparatively higher, at 19%. The model was subsequently applied for long-term simulations (2010–2024) to estimate soybean yield and water requirements for two dominant soil types, Yutan silty clay loam and Tomek silt loam under three irrigation levels - rainfed, …

Jme4110: Hydro-Excavator Robot, Diane L. Evans, Nathan Fenton, Grant Cole, Nicholas Sipe

Washington University / UMSL Mechanical Engineering Design Project JME 4110

In an environmental situation wherein, diesel has leaked into soil surrounding high voltage lines in an underground confined space where workers cannot access physically and for safety reasons. To alleviate the situation, the project goal is to create a remotely controlled robot, with umbilical attachments. Due to the nature of the environment containing sensitive and electrically charged equipment, mechanical means of removing the soil are not feasible. For this reason, the robot is to remove contaminated soil by using a powerful water sprayer and vacuum combination. Other options for features of this robot are that it uses tracks for movement, …

Jme 4110: Automatic Umbrella Opener, Sam Nieder, Adam Freeman

Washington University / UMSL Mechanical Engineering Design Project JME 4110

Manually opening and closing a patio umbrella can be time consuming and laborious. While there are remote controlled umbrellas for sale, they are expensive and may not be a reasonable option for most people. Our task was to create a device that can be retrofitted onto any patio umbrella, that allows it to be opened and closed remotely.

Jme 4110: Remotely Actuated Rail Switch, Calysta Yalew, Chibale Anwisye, Ron Almira, Andy Sengsavang

Washington University / UMSL Mechanical Engineering Design Project JME 4110

This project focuses on developing a device that will switch the direction of rail by moving the manual rail switch itself. This design consists of two winches strategically placed on either side of the rail switch. It is then attached to a beam clamped to the manual switch and via a button on a phone, can pull the handle left and right. With this device patrons are able to keep safe and railway operations would be more efficient.

Jme 4110: Design And Evaluation Of A Remote-Operated Tool For High-Ceiling Light Bulb Maintenance, Faiq Kureshi, Austin Carr, Phuc Truong, Klevis Malaj

Washington University / UMSL Mechanical Engineering Design Project JME 4110

Changing light bulbs in high ceilings is a common but hazardous task. Existing tools are awkward, unstable, and often lead to dropped bulbs, damaged fixtures, or the need for ladders, increasing both risk and effort. There is a clear need for a safe, accurate, and efficient solution that allows bulb replacement from the ground without compromising speed or precision

Optimization Of Thermoelectric Energy Harvester To Power Wireless Onboard Bearing Health Sensors During Rail Service, Danna Cecilia Capitanachi Avila

Theses and Dissertations

This work presents the optimization of a thermoelectric energy harvester designed to supply power to a wireless onboard bearing health monitoring device for critical freight rail components. The study demonstrates an enhancement in the circuitry through the integration of an energy management subsystem (E-Peas AEM20940), which features a boost converter with lower self-start voltage. The harvesting system was tested on dynamic bearing test rigs to closely replicate field service conditions. Test plans were conducted using common freight speeds and loads to simulate a representative urban route. The thermoelectric energy harvester was able to operate at temperature differentials as low as …

Towards Accountability: A Primer On The Space Debris Problem And An Overview Of The Legal Issues Surrounding It, William Schonberg

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Since 1957, the near-Earth population of trackable space objects has grown in number to over 36,000. Of these 36,000+ trackable objects now in low Earth orbit, just a few thousand are working spacecraft. The rest are Earth-orbiting objects which are no longer operational and are considered to be space junk. Because this junk can no longer receive maneuvering commands from its Earth-based owners, the survivability of other spacecraft traveling through or operating in Earth orbit can be jeopardized by the impacts of any number of pieces of this space junk, whose origins can usually be traced back to defunct satellites. …

Electron Beam Irradiation Effects On Bulk Metals And High Entropy Alloys (Crmnv And Crmntiv) And The Synthesis And Characterization Of Irradiation-Induced Damage In Polycrystalline Metal And High Entropy Alloy Thin Films, Najmin Ara Sultana

Mechanical & Aerospace Engineering Theses & Dissertations

Accelerator beam exit windows are designed to withstand intense radiation and mechanical stress while preserving beam transmission and structural integrity. This dissertation explores the structural and mechanical responses of pure metals and high-entropy alloys (HEAs), in both bulk and thin-film forms, under high-dose electron beam (e-beam) irradiation to identify optimal materials for next-generation accelerator exit windows. Monte Carlo simulations using FLUKA, conducted at Jefferson Lab, revealed that amongst Ni, Ti, Cr, and V, Ni exhibits the highest power dissipation, while Ti depicts the lowest power dissipation, with Cr and V demonstrating intermediate characteristics. Bulk polycrystalline (PC) and single-crystalline (SC) samples …