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Articles 82951 - 82980 of 196765

Full-Text Articles in Engineering

Electrospinning: Polymer Nanofibre Adsorbent Applications For Metal Ion Removal, O. K. Pereao, C. Bode-Aluko, G. Ndayambaje, O. Fatoba, L. F. Petrik Dec 2017

Electrospinning: Polymer Nanofibre Adsorbent Applications For Metal Ion Removal, O. K. Pereao, C. Bode-Aluko, G. Ndayambaje, O. Fatoba, L. F. Petrik

Mining Engineering Faculty Research & Creative Works

In recent decades, electro spun nanofibers have been applied to different potential fields such as sensor devices, tissue engineering scaffolds, wound dressing, filtration, drug delivery, etc. and significant review papers have been published in these fields. However, the use of electro spun nanofibers in metal ion adsorption is emerging and comparatively innovative. Nanofiber materials hold great potential in advancing the growth of metallurgical technologies and progress for the separation of metallic ions from various sources. The Physico-chemical removal processes of adsorption use nanofibers due to their large surface area to volume ratio and the availability of a wide variety of …


Special Section On Wireless Emc And Wireless Ota Measurements, Jun Fan, Y. Qi Dec 2017

Special Section On Wireless Emc And Wireless Ota Measurements, Jun Fan, Y. Qi

Electrical and Computer Engineering Faculty Research & Creative Works

No abstract provided.


Investigation On Ti6al4v-V-Cr-Fe-Ss316 Multi-Layers Metallic Structure Fabricated By Laser 3d Printing, Wei Li, Frank W. Liou, Joseph William Newkirk, Karen M. Brown Taminger, William J. Seufzer Dec 2017

Investigation On Ti6al4v-V-Cr-Fe-Ss316 Multi-Layers Metallic Structure Fabricated By Laser 3d Printing, Wei Li, Frank W. Liou, Joseph William Newkirk, Karen M. Brown Taminger, William J. Seufzer

Mechanical and Aerospace Engineering Faculty Research & Creative Works

Joining titanium alloy and stainless steel is becoming an urgent need since their outstanding mechanical properties can be utilized integratedly. However, direct fusion joining of Ti6Al4V to SS316 can cause brittle Ti-Fe intermetallics which compromise join bonds’ mechanical properties. In this research, Laser 3D Printing was applied to explore a new Ti6Al4V to SS316 multi-metallic structure. A novel filler transition route was introduced (Ti6Al4V → V → Cr → Fe → SS316) to avoid the Ti-Fe intermetallics. Two experimental cases were performed for comparison to evaluate this novel route’s effect. In the first case, SS316 layer was directly deposited on …


Structural Responses Of Metallic Glasses Under Neutron Irradiation, L. Yang, H. Y. Li, P. W. Wang, S. Wu, G. Guo, B. Liao, Q. L. Guo, X. Q. Fan, P. Huang, H. B. Lou, F. M. Guo, Q. Zeng, T. Sun, Y. Ren, Lianyi Chen Dec 2017

Structural Responses Of Metallic Glasses Under Neutron Irradiation, L. Yang, H. Y. Li, P. W. Wang, S. Wu, G. Guo, B. Liao, Q. L. Guo, X. Q. Fan, P. Huang, H. B. Lou, F. M. Guo, Q. Zeng, T. Sun, Y. Ren, Lianyi Chen

Mechanical and Aerospace Engineering Faculty Research & Creative Works

No abstract provided.


Nanoparticle-Based Plasmonic Transduction For Modulation Of Electrically Excitable Cells, Parveen Bazard, Robert D. Frisina, Joseph P. Walton, Venkat R. Bhethanabotla Dec 2017

Nanoparticle-Based Plasmonic Transduction For Modulation Of Electrically Excitable Cells, Parveen Bazard, Robert D. Frisina, Joseph P. Walton, Venkat R. Bhethanabotla

Chemical and Biochemical Engineering Faculty Research & Creative Works

There is a compelling need for the development of new sensory and neural prosthetic devices which are capable of more precise point stimulation. Current prosthetic devices suffer from the limitation of low spatial resolution due to the non-specific stimulation characteristics of electrical stimulation, i.e., the spread of electric fields generated. We present a visible light stimulation method for modulating the firing patterns of electrically excitable cells using surface plasmon resonance phenomena. In in-vitro studies using gold (Au) nanoparticle-coated nanoelectrodes, we show that this method (substrate coated with nanoparticles) has the potential for incorporating this new technology into neural stimulation prosthetics, …


Dynamic Mechanical Properties And Microstructure Of Graphene Oxide Nanosheets Reinforced Cement Composites, Wujian Long, Jingjie Wei, Hongyan Ma, Feng Xing Dec 2017

Dynamic Mechanical Properties And Microstructure Of Graphene Oxide Nanosheets Reinforced Cement Composites, Wujian Long, Jingjie Wei, Hongyan Ma, Feng Xing

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

This paper presents an experimental investigation on the effect of uniformly dispersed graphene oxide (GO) nanosheets on dynamic mechanical properties of cement based composites prepared with recycled fine aggregate (RFA). Three different amounts of GO, 0.05%, 0.10%, and 0.20% in mass of cement, were used in the experiments. The visual inspections of GO nanosheets were also carried out after ultrasonication by transmission electron microscope (TEM) atomic force microscope (AFM), and Raman to characterize the dispersion effect of graphite oxide. Dynamic mechanical analyzer test showed that the maximum increased amount of loss factor and storage modulus, energy absorption was 125%, 53%, …


Effect Of Explosive Charge Geometry On Shockwave Propagation, Catherine E. Johnson, Phillip R. Mulligan, Kelly Williams, Martin Langenderfer, Jeffrey Heniff Dec 2017

Effect Of Explosive Charge Geometry On Shockwave Propagation, Catherine E. Johnson, Phillip R. Mulligan, Kelly Williams, Martin Langenderfer, Jeffrey Heniff

Mining Engineering Faculty Research & Creative Works

With advancements in the 3D printing of explosives, the understanding how the shape of an explosive charge influences the energy expansion and subsequently the focusing of energy is becoming increasingly more important. Theoretically, explosive charges could be printed to focus the energy on targets of interest or to synthesize nanomaterials. The first step to quantifying the energy expansion and shock wave convergences for a 3D printed charge is to examine simple geometries of traditional explosive charges (non-3D printed). Detonation of 15 PETN-based primasheet charges in the spherical, cubic, cylindrical, and tetrahedral configurations have been examined in this study. Qualitative data …


Process Models Discovery And Traces Classification: A Fuzzy-Bpmn Mining Approach., Kingsley Okoye Dr, Usman Naeem Dr, Syed Islam Dr, Abdel-Rahman H. Tawil Dr, Elyes Lamine Dr Dec 2017

Process Models Discovery And Traces Classification: A Fuzzy-Bpmn Mining Approach., Kingsley Okoye Dr, Usman Naeem Dr, Syed Islam Dr, Abdel-Rahman H. Tawil Dr, Elyes Lamine Dr

Journal of International Technology and Information Management

The discovery of useful or worthwhile process models must be performed with due regards to the transformation that needs to be achieved. The blend of the data representations (i.e data mining) and process modelling methods, often allied to the field of Process Mining (PM), has proven to be effective in the process analysis of the event logs readily available in many organisations information systems. Moreover, the Process Discovery has been lately seen as the most important and most visible intellectual challenge related to the process mining. The method involves automatic construction of process models from event logs about any domain …


Developing A Digital Contents Valuing Model: How Users Appreciate Their Values, Changsu Kim, Jongheon Kim Dec 2017

Developing A Digital Contents Valuing Model: How Users Appreciate Their Values, Changsu Kim, Jongheon Kim

Journal of International Technology and Information Management

The principal objective of this paper was to propose and verify a digital content valuing model, which is expected to perform a significant role in future research, and provide novel and practical implications. For the efficacy of a model for the evaluation of digital content value, this study reviewed digital content value and categorized it into intrinsic, interaction, and business value. Based on the research model, we attempted to identify and assess the effects of intrinsic digital content value on digital content interaction value, and to characterize the relationship between digital content interaction value and digital content business value. Consequently, …


Data Protection In Nigeria: Addressing The Multifarious Challenges Of A Deficient Legal System, Roland Akindele Dec 2017

Data Protection In Nigeria: Addressing The Multifarious Challenges Of A Deficient Legal System, Roland Akindele

Journal of International Technology and Information Management

This paper provides an overview of the current state of privacy and data protection policies and regulations in Nigeria. The paper contends that the extant legal regime in Nigeria is patently inadequate to effectively protect individuals against abuse resulting from the processing of their personal data. The view is based on the critical analysis of the current legal regime in Nigeria vis-à-vis the review of some vital data privacy issues. The paper makes some recommendations for the reform of the law.


Whitespace Exploration, Jason Lloyd Daniel Dec 2017

Whitespace Exploration, Jason Lloyd Daniel

Master's Theses

As engineering systems grow in complexity so too must the design tools that we use evolve and allow for decision makers to efficiently ask questions of their model and obtain meaningful answers. The process of whitespace exploration has recently been developed to aid in engineering design and provide insight into a design space where traditional design exploration methods may fail. In an effort to further the research and development of whitespace exploration algorithms, a software package called Thalia has been created to allow for automated data collection and experimentation with the whitespace exploration methodology.

In this work, whitespace exploration is …


Rf Front End For An Integrated Silhouette Capture And Boundary Detection Frequency Modulated Continuous Wave Ultra-Wideband Radar System For The Extension Of Independent Living, Adrian Smet Dec 2017

Rf Front End For An Integrated Silhouette Capture And Boundary Detection Frequency Modulated Continuous Wave Ultra-Wideband Radar System For The Extension Of Independent Living, Adrian Smet

Master's Theses

Limitations of current eldercare monitoring systems leave a need for new solutions. A monitoring system based on a frequency modulated continuous wave ultra-wideband short-range radar is proposed for this application. The complete proposed monitoring system is comprised of four blocks: boundary detection, silhouette capture, human identification, and data transmission. This paper develops the RF front end hardware for the silhouette capture subsystem.

System requirements are derived for the silhouette capture subsystem. An architecture for the RF front end is designed, and required individual component specifications are determined. Components are selected off the shelf or custom designed for each socket. Full …


Eeg Characterization During Motor Tasks That Are Difficult For Movement Disorder Patients, Adam Joshua Aslam Dec 2017

Eeg Characterization During Motor Tasks That Are Difficult For Movement Disorder Patients, Adam Joshua Aslam

Master's Theses

Movement disorders are a group of syndromes that often arise due to neurological abnormalities. Approximately 40 million Americans are affected by some form of movement disorder, significantly impacting patients’ quality of life and their ability to live independently. Deep brain stimulation (DBS) is one treatment that has shown promising results in the past couple decades, however, the currently used open-loop system has several drawbacks. By implementing a closed-loop or adaptive DBS (aDBS) system, the need for expensive parameter reprogramming sessions would be reduced, side-effects may be relieved, and habituation could be avoided. Several biomarkers, for example signals or activity derived …


Interfacing A Hirudo Medicinalis Retzius Cell With Insulated Gate Of Mosfet, Rachel M. Smith Dec 2017

Interfacing A Hirudo Medicinalis Retzius Cell With Insulated Gate Of Mosfet, Rachel M. Smith

Master's Theses

Much work has been done to study the external stimulation of nervous tissue as well as the transmission of neural signals to electronics. Peter Fromherz was one of the pioneers in this area of electrophysiology, with a series of experiments in the 1990s that aimed to characterize and optimize the interface between neural tissue and transistors. In this thesis, Kurt Sjoberg and I interfaced a Retzius cell isolated from a Hirudo medicinalis ganglion with the insulated gate of a MOSFET. The goal was to see change in membrane potential that could be related Fromherz’s original 1991 work. Our experimental setup …


Development Of A Prototype Detection System To Investigate The Fission Mechanism Of Fermium (Fm) Isotopes, P K Rath Dec 2017

Development Of A Prototype Detection System To Investigate The Fission Mechanism Of Fermium (Fm) Isotopes, P K Rath

Manipal Journal of Science and Technology

The development of a prototype detection unit for measuring fission events in-beam is currently underway. Measurements associated with fission events include fission-fragment mass distributions (FFMD), fission-fragment angular distributions (FFAD), and total kinetic energy distribution (TKE). A systematic study of fission of the Fermium isotopes, 244Fm to 256Fm is being undertaken to investigate the dynamics of fission in this mass region. Novel results from this systematic study are expected for this uncharted set of nucleus, which shows a unique transitional asymmetric to symmetric fission mass distribution.


In House Development Of A Scalar Magnetometer, Prasanna Mahavarkar Dec 2017

In House Development Of A Scalar Magnetometer, Prasanna Mahavarkar

Manipal Journal of Science and Technology

Scalar magnetometers namely the proton precession magnetometers (PPM) are popular in various ground surveys and observatories. With this primary purpose of generating the ground based magnetic data, the Indian Institute of Geomagnetism (IIG) for the last three decades have been developing low cost PPM. Beginning with the 1 nT PPM, which has undergone several changes in design, the successor 0.1 nT PPM – the advanced version has been successfully developed and is installed at various observatories of the institute. The 1 nT version is a manual operated PPM commissioned in the institute observatories to record the absolute geomagnetic field and …


Efficient Planning Of Resources And Utilization Of Alternative Building Materials And Techniques For A Residential Construction Project, V Srinivasa Raghavan Dec 2017

Efficient Planning Of Resources And Utilization Of Alternative Building Materials And Techniques For A Residential Construction Project, V Srinivasa Raghavan

Manipal Journal of Science and Technology

Major research efforts are being focused on the significant usage of the alternative building materials, techniques, and on resource optimization for improving the effectiveness of cost and time of a construction project. Unless proper resources are planned and procured, no activity can be executed according to a prefixed time schedule. Project managers must take complex decisions under uncertainty conditions that sometimes extend beyond task durations. This paper provides an overview of the study dealings with the usage of alternative building materials, techniques, and resource optimization. The study has been carried out as two parts. In the first part, factors affecting …


Knowledge Flows And Influence In Online Social Networks: Proposing A Research Agenda, Charles M. Weber, Nitin V. Mayande Dec 2017

Knowledge Flows And Influence In Online Social Networks: Proposing A Research Agenda, Charles M. Weber, Nitin V. Mayande

Engineering and Technology Management Faculty Publications and Presentations

Online social networks, which have been defined as aggregated organizations that emerge from the Internet when people carry on public discussions, are increasingly becoming the vehicle of influence in social, political and economic discourse. Yet, despite its increasing importance, the nature of influence in online social networks is not really understood. Practitioners openly admit that they lack the experience to make sense of the phenomenon, and extant theory of influence in networks, which extrapolates from observations of the real world, is demonstrably inadequate when it comes to explaining influence online. The paper introduces a novel approach to analyzing influence online, …


Benchmarking Of Technology Roadmapping Process In Energy Sector: A Literature Review, Chih-Jen Yu, Tugrul Unsal Daim Dec 2017

Benchmarking Of Technology Roadmapping Process In Energy Sector: A Literature Review, Chih-Jen Yu, Tugrul Unsal Daim

Engineering and Technology Management Faculty Publications and Presentations

Technology roadmapping (TRM) has been recognized as an effective and flexible technology planning tool to assist company, industry, or nations to layout their strategic technology needs and align their vision and strategic objectives. Technology roadmap have been developed into various types and formats, by means of generic or customized processes, to suit specific organizational requirement in different industry settings such as energy sector. However, evaluating the effectiveness of roadmapping process remains a critical issue for roadmap updating and improvement. This paper proposes a benchmark approach based on a literature review to help develop a comparative model with required checklists.


Rheological Properties Considering The Effect Of Aggregates On Concrete Slump Flow, Tae Yong Shin, Jae Hong Kim, Seong Ho Han Dec 2017

Rheological Properties Considering The Effect Of Aggregates On Concrete Slump Flow, Tae Yong Shin, Jae Hong Kim, Seong Ho Han

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Rheology of concrete allows us to understand the flow behavior of concrete and further extend the quantitative evaluation of its construction performance. The use of a concrete rheometer is promising for the purpose but sometimes limited high associated cost and procedure complexity. This study proposes a simulation-based model that correlates the slump flow test results to a concrete's rheological properties, allowing quantitative evaluation through this simple method. The proposed model is based on single-fluid simulation using the volume-of-fluid method, with an extension to accommodate the partial segregation of coarse aggregates. Either the channel flow or the L-shaped panel filling of …


Crustal Thickness Beneath Libya And The Origin Of Partial Melt Beneath As Sawda Volcanic Province From Receiver Function Constraints, Awad A. Lemnifi, Abdelsalam Elshaafi, John Browning, Nassib Aouad, Saad K. El Ebaidi, Kelly K. Liu, Agust Gudmundsson Dec 2017

Crustal Thickness Beneath Libya And The Origin Of Partial Melt Beneath As Sawda Volcanic Province From Receiver Function Constraints, Awad A. Lemnifi, Abdelsalam Elshaafi, John Browning, Nassib Aouad, Saad K. El Ebaidi, Kelly K. Liu, Agust Gudmundsson

Mining Engineering Faculty Research & Creative Works

This study investigates crustal thickness and properties within the Libyan region. Results obtained from 15 seismic stations belonging to the Libyan Center for Remote Sensing and Space Science are reported, in addition to 3 seismic stations publically available, using receiver functions. The results show crustal thicknesses ranging from 24 km to 36 km (with uncertainties ranging between ±0.10 km and ±0.90 km). More specifically, crustal thickness ranges from 32 km to 36 km in the southern portion of the Libyan territory then becomes thinner, between 24 km and 30 km, in the coastal areas of Libya and thinnest, between 24 …


Recursive Non-Local Means Filter For Video Denoising, Redha A. Ali, Russell C. Hardie Dec 2017

Recursive Non-Local Means Filter For Video Denoising, Redha A. Ali, Russell C. Hardie

Electrical and Computer Engineering Faculty Publications

In this paper, we propose a computationally efficient algorithm for video denoising that exploits temporal and spatial redundancy. The proposed method is based on non-local means (NLM). NLM methods have been applied successfully in various image denoising applications. In the single-frame NLM method, each output pixel is formed as a weighted sum of the center pixels of neighboring patches, within a given search window.

The weights are based on the patch intensity vector distances. The process requires computing vector distances for all of the patches in the search window. Direct extension of this method from 2D to 3D, for video …


Model Predictive Power Management Of A Hybrid Electric Propulsion System For Aircraft, Tyler J. Wall Dec 2017

Model Predictive Power Management Of A Hybrid Electric Propulsion System For Aircraft, Tyler J. Wall

Masters Theses

This work presents the switched optimal power flow control for an aircraft with a hybrid electric propulsion system. The propulsion system is a switched system that operates in either of two modes: (i) battery discharging and electric motor propelling and (ii) battery charging and electric motor generating. The aircraft model and components that form the hybrid propulsion system are modeled as either an algebraic power source/sink or as a dynamic model with appropriate power and state interconnections. With the system model defined, a model predictive control power management strategy is set fourth which minimizes a performance index that includes altitude …


Visualizing The Constructability Of A Steel Structure Using Building Information Modeling And Game Simulation, Mohammed Al Dafaay Dec 2017

Visualizing The Constructability Of A Steel Structure Using Building Information Modeling And Game Simulation, Mohammed Al Dafaay

Masters Theses

This research aims to visualize a steel structural design to: (1) analyze its constructability, and (2) support collaboration between project participants at the planning stage. The visualization uses Building Information Modeling (BIM) and game engine simulation to develop a realistic interactive simulation for constructability analysis. A case study experiment was conducted on visualizing the erection process of a steel structure to facilitate the understanding of needed construction operation. The simulation incorporated construction domain knowledge through various entity components and predefined interaction rules. Results showed that through such a visualization method, field operation can be observed in real-time at the planning …


Fiscal Year 2017 Fhwa-536 Report For The Kentucky Transportation Cabinet, Steven Douglas Kreis, Candice Y. Wallace, Bryan Gibson, Sarah M. Mccormack Dec 2017

Fiscal Year 2017 Fhwa-536 Report For The Kentucky Transportation Cabinet, Steven Douglas Kreis, Candice Y. Wallace, Bryan Gibson, Sarah M. Mccormack

Kentucky Transportation Center Research Report

The Federal Highway Administration (FHWA) requires state transportation agencies to submit a biennial report on local highway finances. The purpose of these reports is to provide FHWA with the data it needs to capture the financing of highway activities at the local level. Based on this information, it can identify trends in revenue, expenditures, investments, and program development, and in turn make decisions about future investments. The report, FHWA-536, asks agencies to report on four areas of local highway finance: 1) disposition of highway-user revenues; 2) revenues used for roads and streets identified by source and funding type; 3) road …


Applicability Of Zipper Merge Versus Early Merge In Kentucky Work Zones, Erin Lammers-Staats, Jerry G. Pigman, Brian K. Howell, Adam J. Kirk Dec 2017

Applicability Of Zipper Merge Versus Early Merge In Kentucky Work Zones, Erin Lammers-Staats, Jerry G. Pigman, Brian K. Howell, Adam J. Kirk

Kentucky Transportation Center Research Report

In an effort to improve work zone safety and streamline traffic flows, a number of state transportation agencies (STAs) have experimented with the zipper merge. The zipper merge differs from a conventional, or early, merge in that vehicles do not merge into the lane that remains open immediately after being notified of a lane closure. Rather, vehicles continue to occupy all lanes until they reach the taper, at which point — and directed by signage — vehicles take turns merging into the open lane, creating a zipper pattern. At the request of the Kentucky Transportation Cabinet (KYTC), Kentucky Transportation Center …


Anti-Icing Properties Of Femtosecond Laser Surface Processed Material, Ryan James Bell Dec 2017

Anti-Icing Properties Of Femtosecond Laser Surface Processed Material, Ryan James Bell

Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research

In this thesis, the use of femtosecond laser surface processing (FLSP) to enhance the anti-icing properties of a commonly used aircraft alloy, Al 7075-O Clad is described. By changing the surface morphology through FLSP and the surface chemistry through siloxane vapor deposition, the wettability of Al 7075-O Clad was altered.

Condensation and the subsequent freezing of condensates on FLSP Al 7075-O Clad was studied. Both structure height and surface wettability were shown to play a role in the delay of freezing. Freezing occurred on the FLSP superhydrophilic surface faster than on the unprocessed Al 7075-O Clad surface, however, freezing was …


Condensation And Subsequent Freezing Delays As A Result Of Using A Femtosecond Laser Functionalized Surface, Chongji Huang Dec 2017

Condensation And Subsequent Freezing Delays As A Result Of Using A Femtosecond Laser Functionalized Surface, Chongji Huang

Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research

Femtosecond laser surface processing (FLSP) enhances the anti-icing properties of a commonly used aircraft alloy, Al 7075-O Clad. The wettability of Al 7075-O Clad was altered by changing the surface morphology through FLSP and the surface chemistry through siloxane vapor deposition. Tall mound and short mound FLSP functionalized surfaces were created through two sets of laser parameters. Atmospheric condensation and subsequent freezing of condensates on FLSP Al 7075-O Clad was studied. Results indicate that both structure height and surface wettability play a role in the delay of freezing. Freezing occurred on the FLSP superhydrophilic surface faster than on a unprocessed …


Design And Implementation Of A Stand-Alone Tool For Metabolic Simulations, Milad Ghiasi Rad Dec 2017

Design And Implementation Of A Stand-Alone Tool For Metabolic Simulations, Milad Ghiasi Rad

School of Computing: Dissertations, Theses, and Student Research

In this thesis, we present the design and implementation of a stand-alone tool for metabolic simulations. This system is able to integrate custom-built SBML models along with external user’s input information and produces the estimation of any reactants participating in the chain of the reactions in the provided model, e.g., ATP, Glucose, Insulin, for the given duration using numerical analysis and simulations. This tool offers the food intake arguments in the calculations to consider the personalized metabolic characteristics in the simulations. The tool has also been generalized to take into consideration of temporal genomic information and be flexible for simulation …


Blue-Light Induced Biosynthesis Of Ros Contributes To The Signaling Mechanism Of Arabidopsis Cryptochrome, Mohamed A. El-Esawi, Louis David Arthaut, Nathalie Jourdan, Alain D'Harlingue, Justin J. Link, Margaret Ahmad, Carlos F. Martino Dec 2017

Blue-Light Induced Biosynthesis Of Ros Contributes To The Signaling Mechanism Of Arabidopsis Cryptochrome, Mohamed A. El-Esawi, Louis David Arthaut, Nathalie Jourdan, Alain D'Harlingue, Justin J. Link, Margaret Ahmad, Carlos F. Martino

Biomedical Engineering and Sciences Faculty Publications

Cryptochromes are evolutionarily conserved blue light receptors with many roles throughout plant growth and development. They undergo conformational changes in response to light enabling interaction with multiple downstream signaling partners. Recently, it has been shown that cryptochromes also synthesize reactive oxygen species (ROS) in response to light, suggesting the possibility of an alternate signaling mechanism. Here we show by fluorescence imaging and microscopy that H202 and ROS accumulate in the plant nucleus after cryptochrome activation. They induce ROS-regulated transcripts including for genes implicated in pathogen defense, biotic and abiotic stress. Mutant cryptochrome alleles that are non-functional in photomorphogenesis retain the …