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Articles 13231 - 13260 of 198037

Full-Text Articles in Engineering

Experimental Investgation Of Core Fluid And Heat Transfer Of Pwr By Using Advanced Measurments Techniques, Saud Aldawood Jan 2025

Experimental Investgation Of Core Fluid And Heat Transfer Of Pwr By Using Advanced Measurments Techniques, Saud Aldawood

Doctoral Dissertations

This comprehensive study investigates the thermal-hydraulic mechanisms and convective heat transfer characteristics within (PWRs), with a focus on (SMRs) under both single-phase and two-phase bubbly flow conditions. Specifically, the research investigates the complex bubble dynamics in two-phase flow rod bundles, which are crucial for understanding reactor behavior during loss-of-coolant accidents (LOCA). Employing an advanced experimental setup developed at the (mFReel) at MST, this work measures critical parameters such as void fraction, bubble passage frequency, bubble velocity, bubble chord length, and interfacial area concentration (IAC) using a four-tip optical probe across various locations in the rod bundle. The findings reveal that …


On The Importance Of Novel Adsorption Processes And Structured Adsorbents For Biogas Upgrading And Storage, Kyle Newport Jan 2025

On The Importance Of Novel Adsorption Processes And Structured Adsorbents For Biogas Upgrading And Storage, Kyle Newport

Doctoral Dissertations

The current adsorption processes investigated for biogas upgrading encounter severe challenges with regards to both adsorbents and processes, which necessitate the design and development of novel approaches to effectively separate carbon dioxide from methane and, hence, produce pipeline-grade renewable natural gas (RNG) as a fuel. These key challenges include (i) moderate CO2/CH4 selectivity, (ii) relatively long cycle times, (iii) high regeneration energy requirements, (iv) high-pressure drop, and (v) short adsorbent lifetime. The current biogas upgrading processes typically rely on pressure swing adsorption (PSA) or temperature swing adsorption (TSA). While PSA cycles are fast, the system requires large amounts of energy …


A Comprehensive Analysis Of Climate Resilience Strategies For Small Island Developing States, Ashley-Ann Davis Jan 2025

A Comprehensive Analysis Of Climate Resilience Strategies For Small Island Developing States, Ashley-Ann Davis

Doctoral Dissertations

Small Island Developing States (SIDS) face unique and disproportionate challenges in their efforts to achieve climate resilience due to their geographic vulnerabilities, limited resources, and systemic economic and social constraints. This dissertation provides a comprehensive analysis of climate resilience strategies tailored to SIDS, addressing critical systemic issues and exploring interdisciplinary solutions. It synthesizes findings across four interconnected studies, including an analysis of disaster preparedness and response mechanisms in developing countries to identify gaps and opportunities for improving resilience in extreme events. The research investigates economic factors influencing energy portfolio transitions in the Caribbean, emphasizing the complexities of renewable energy adoption. …


Deep Learning Architecture Design For Nano-Satellite Image Super-Resolution, William Everette Symolon Jan 2025

Deep Learning Architecture Design For Nano-Satellite Image Super-Resolution, William Everette Symolon

Doctoral Dissertations

Increasing threats to U.S. national security satellite constellations have resulted in an increased interest in constellation resilience and satellite redundancy. NanoSats have contributed to commercial, scientific and government applications in remote sensing, communications, navigation, and research. They also have the potential to enhance satellite constellation resilience. However, the inherent size, weight, and power limitations of NanoSats enforce constraints on imaging hardware; the small lenses and short focal lengths result in imagery with low spatial resolution, which limits the utility of CubeSat images for military planning purposes and national intelligence applications. This research proposed a deep learning architecture capable of enhancing …


Synthesis Of Graphene Using Carbonaceous Materials In An Ultrasonic Reactor, Paul Chukwuma Ani Jan 2025

Synthesis Of Graphene Using Carbonaceous Materials In An Ultrasonic Reactor, Paul Chukwuma Ani

Doctoral Dissertations

The synthesis of high-quality graphene from sustainable carbonaceous feedstocks offers an avenue to reduce the environmental and economic costs associated with conventional graphite-based production. This research investigates the co-utilization of biochar and graphite as precursors for graphene fabrication in an ultrasonic reactor. Biochar was produced from biomass via downdraft gasification at 850 °C, yielding a high fixed-carbon, partially graphitized material with favorable surface area and porosity. Graphite, selected for its crystalline structure, was investigated with biochar to assess the influence of precursor composition on exfoliation efficiency, layer thickness distribution, and defect density. Ultrasonic-assisted liquid-phase exfoliation was employed, with process parameters …


Application Of Gurney And Flight Of Fragment Calculations For Water Jet Velocities In Explosive Applications, Rachel L. Bauer, Tate B. Friedrich, Catherine E. Johnson Jan 2025

Application Of Gurney And Flight Of Fragment Calculations For Water Jet Velocities In Explosive Applications, Rachel L. Bauer, Tate B. Friedrich, Catherine E. Johnson

Mining Engineering Faculty Research & Creative Works

This study investigates the application of Gurney and flight of fragment equations, typically used to predict metal fragment velocities, in modeling the water jet behavior. Three shotgun cartridge sizes were used as the energy source: 2.59 g, 5.83 g, and 7.13 g. Two configurations were tested: standard (full-barrel water load) and "negative 8" (partial water load). High-speed footage captured water column velocities, and Gurney models, including infinitely tamped and open-faced configurations, combined with the flight of fragment model were used to assess prediction accuracy. Results showed charge strength significantly affects water column velocity, with higher strengths yielding greater stability and …


Viscoelastic Materials Evaluated For Blast-Resistant Designs, M. Sutter, C. Thomas, A. D. Douglas, F. Dogan, C. E. Johnson Jan 2025

Viscoelastic Materials Evaluated For Blast-Resistant Designs, M. Sutter, C. Thomas, A. D. Douglas, F. Dogan, C. E. Johnson

Mining Engineering Faculty Research & Creative Works

Viscoelastic materials have extensive military applications due to their energy absorption capabilities, with the potential to reduce blast energy imposed on buildings, vehicles, and personnel. Based on current literature, limited information is available regarding the mitigation of blast energy related to these uses. The impact of thickness, nanoparticle addition, and layering variation was assessed in this study using commercially available viscoelastic materials in open-air blasts of Composition C4 to determine shock energy mitigation capabilities. Time-pressure waveforms were recorded to identify optimal changes in shock wave characteristics: reduced peak pressure, positive phase duration, and impulse, with increased rise times. Results were …


Optimizing Hy Wet Ball Mill Efficiency Using Response Surface Methodology, Peng Chen, Ying Hou, Hongxu Lv, Ahmed Sobhy Jan 2025

Optimizing Hy Wet Ball Mill Efficiency Using Response Surface Methodology, Peng Chen, Ying Hou, Hongxu Lv, Ahmed Sobhy

Mining Engineering Faculty Research & Creative Works

This study uses response surface methodology (RSM) with a central composite design to optimize the HY wet ball mill work index. Key operational parameters—ore quantity, water addition, and media proportion—were systematically investigated. Variance analysis identified these factors as statistically significant (p < 0.0001), with ore quantity exhibiting the strongest influence (F value = 82.67). Optimal conditions (water addition: 1083.880 mL, media filling rate: 40.362%, ore quantity: 1827.130 g) achieved a minimum work index of 10.195 kW h t−1. Validation tests confirmed the model's accuracy, yielding an average work index of 10.168 kW h t−1 (deviation: 0.027 kW h t−1). Compared to single-factor tests, RSM optimization reduced energy consumption by 3.76% (0.3975 kW h t−1), translating to annual savings of $214,000 for a mid-sized processing plant. This demonstrates RSM's efficacy in …


Using Telemetry To Assess Operator Effects On Hydraulic Shovel Energy Efficiency. Part Ii: Assessing Operator Effects, Noah Adekunle Aluko, Kwame Awuah-Offei Jan 2025

Using Telemetry To Assess Operator Effects On Hydraulic Shovel Energy Efficiency. Part Ii: Assessing Operator Effects, Noah Adekunle Aluko, Kwame Awuah-Offei

Mining Engineering Faculty Research & Creative Works

In this second part of a two-part series, this study investigates whether operator practices significantly affect the energy efficiency of hydraulic shovels, using detailed telemetry data. Rather than assuming this relationship, the research systematically tests it through a combination of statistical and regression analyses. First, Welch's and Kruskal–Wallis ANOVA confirm that operators differ significantly in their energy efficiency (p = 0.0000). Next, correlation analysis links parameters identified in Part I to energy per unit loading rate, and difference regression analysis determines which parameters most strongly influence efficiency variations. The results show that differences in payloads are the primary driver of …


Understanding The Usefulness Of Self-Escape Technologies In Underground Mining: Perspectives Of Metal/Nonmetal Miners, Eugene A. Gyawu, Kwame Awuah-Offei, D. A. Baker Jan 2025

Understanding The Usefulness Of Self-Escape Technologies In Underground Mining: Perspectives Of Metal/Nonmetal Miners, Eugene A. Gyawu, Kwame Awuah-Offei, D. A. Baker

Mining Engineering Faculty Research & Creative Works

Research on mine self-escape often focuses on coal mining, while perspectives from underground metal/nonmetal miners remain understudied despite their distinct emergency response challenges and unique operating environments. Using a scenario-based survey approach, this study evaluated underground metal/nonmetal miners' perceptions of the usefulness of 18 hypothetical self-escape interventions and how these perceptions are influenced by worker characteristics. Employment type was the strongest predictor of usefulness ratings, with hourly employees rating several self-escape interventions significantly higher than salaried employees, including those related to improving self-contained self-rescuers (SCSRs) and tethered guidance systems. The data suggested potential trends where perceived usefulness increased with more …


Navigation In Underground Mine Environments: A Simulation Framework For Quadruped Robots, Yixiang Gao, Kwame Awuah-Offei Jan 2025

Navigation In Underground Mine Environments: A Simulation Framework For Quadruped Robots, Yixiang Gao, Kwame Awuah-Offei

Mining Engineering Faculty Research & Creative Works

Quadruped robots have shown significant potential for navigating complex and hazardous environments, such as underground mines, where traditional wheeled or tracked systems have limitations. However, their development and deployment are hindered by the disparity between controlled laboratory testing and real-world conditions and the lack of tools (e.g., simulation testbeds) that expedite the required development and testing. This work develops a simulation testbed for expediting and advancing navigation algorithms, perception systems, and control strategies for quadruped robots in subterranean and hazardous environments. By utilizing high-fidelity 3D maps, ranging from intricate cave systems to real-world sites like the Edgar Mine, simulation environment …


Evaluating Feasibility Of Incorporating Fuel Cell Drive Trains Into Existing Haul Trucks, Ayorinde Akinrinlola, Kwame Awuah-Offei, Abdullah Al Moinee Jan 2025

Evaluating Feasibility Of Incorporating Fuel Cell Drive Trains Into Existing Haul Trucks, Ayorinde Akinrinlola, Kwame Awuah-Offei, Abdullah Al Moinee

Mining Engineering Faculty Research & Creative Works

Hydrogen, as a fuel source in material handling, has become a viable option for achieving carbon reduction targets. The objective of this work was to apply vehicle drive train and energy simulation in MATLAB/Simulink to guide a discussion of the challenges and opportunities of incorporating hydrogen fuel cell drive trains into the current form factors of mine haul trucks. We built and validated a model based on the Komatsu 830-E truck and used it to estimate the hydrogen requirements for typical short-haul, medium-haul, and long-haul cycles; in the short-haul case (cycle time = 852 s) the truck consumed 23.6 L …


Optimizing Acid Mist Suppression: Unraveling Surfactant Effects On Bubble Formation And Bursting Dynamics In Copper Electrowinning, Ashish Kakoria, Mirza Muhammad Zaid, Aamir Iqbal, Ellen Amoako Afful, Guang Xu Jan 2025

Optimizing Acid Mist Suppression: Unraveling Surfactant Effects On Bubble Formation And Bursting Dynamics In Copper Electrowinning, Ashish Kakoria, Mirza Muhammad Zaid, Aamir Iqbal, Ellen Amoako Afful, Guang Xu

Mining Engineering Faculty Research & Creative Works

The working mechanism of surfactant to reduce acid mist in copper electrowinning system is not well understood. Most of the studies are based on the surface tension reduction phenomenon but this is not the only function that causes acid mist reduction. In this paper, we investigated the effect of different surfactants on a bubble's residence time, terminal velocity, flow regime, and bursting dynamics using a high-speed camera. We have evaluated five different surfactants and found that the presence of surfactants reduces the terminal velocity, bubble diameter, and increases the residence time of the bubble in electrolyte. Especially for FC-1100, the …


Cracking The Code: Harnessing Serpentinization-Driven Micro-Crack Networks For Hydrogen Generation And Subsurface Resource Recovery, U. Mutlu, G. Boitnott, A. Lisjak, J. Ha, O. Mahabadi, P. Connolly, B. N. Tunnell, T. Sherizadeh, S. Nowak, J. E. Olson Jan 2025

Cracking The Code: Harnessing Serpentinization-Driven Micro-Crack Networks For Hydrogen Generation And Subsurface Resource Recovery, U. Mutlu, G. Boitnott, A. Lisjak, J. Ha, O. Mahabadi, P. Connolly, B. N. Tunnell, T. Sherizadeh, S. Nowak, J. E. Olson

Mining Engineering Faculty Research & Creative Works

This study focuses on simulating volume-increasing processes caused by natural hydrogen-generating serpentinization, a reaction in which olivine and pyroxene typically transform into serpentine minerals. The volume increase leads to crack propagation along grain interfaces and new crack generation within unreacted crystals (aka grains). The interplay between thermo-hydro-mechanicalchemical (THMC) conditions plays a critical role in the initiation, propagation, and coalescence of various fracture surfaces, which enhances permeability and can accelerate serpentinization and hydrogen production rates. In the scenarios considered, chemical reactions between formation brine and reactive minerals result in volumetric expansion, generating stress and promoting the propagation of micro-cracks. Our results …


Investigation Of Radiation Absorption Behavior Of Banana Leaves And Onion: A Cost-Effective Way Of Radiation Protection, Arpita Datta, Alpana Goel, Shreya Singh Jan 2025

Investigation Of Radiation Absorption Behavior Of Banana Leaves And Onion: A Cost-Effective Way Of Radiation Protection, Arpita Datta, Alpana Goel, Shreya Singh

International Journal of Nuclear Security

Shielding of nuclear radiation is an important component of radiation safety aiming to reduce the exposure of ionizing radiation to radiation workers. In the present work, beta and gamma radiation absorption properties of various environmentally friendly natural materials such as onion, banana leaf, and banana stem were investigated with a view to understand their radiation shielding capability. Pure beta source 90Sr–90Y and gamma sources such as 241Am, 137Cs and 60Co were used to study the attenuation of beta and gamma radiation using these natural materials. Intensity of the emitted radiation was measured by placing …


Optimal Control Of Queueing Systems With Error-Prone Servers, Junqi Hu, Sigrún Andradóttir, Hayriye Ayhan Jan 2025

Optimal Control Of Queueing Systems With Error-Prone Servers, Junqi Hu, Sigrún Andradóttir, Hayriye Ayhan

Information Technology & Decision Sciences Faculty Publications

Consider a Markovian tandem line with finite intermediate buffers and an equal number of stations and servers. Servers are flexible but noncollaborative, so that a job can be processed by at most one server at any time. When a job is being processed, it can be damaged and wasted depending on the proficiency of the server. We identify the dynamic server assignment policy that maximizes the long-run average throughput of the system with two stations and two servers. We find that the optimal policy is either a single or a double threshold policy on the number of jobs in the …


Modeling And Analysis Of Amorphous Steel Transformer For Potential Loss Reduction In Power Systems., Daniel A. Muchow Jan 2025

Modeling And Analysis Of Amorphous Steel Transformer For Potential Loss Reduction In Power Systems., Daniel A. Muchow

Theses and Dissertations--Electrical and Computer Engineering

The energy consumed in power systems can be reduced directly by implementing new technologies and materials. The effort to reduce the carbon emissions expelled from the production of electrical energy has become a major focus. According to the U.S. energy Information Administration approximately 5% of all the electric generated and transmitted in the U.S. electrical grid is lost. The energy profile calculated from the usage of electrical power from all 50 states in 2021 is estimated to be 3.8 billion megawatts per hour, with a state average of just over 11 cents per MWh, producing just over 41.8 billion in …


Analysis And Design Optimization Of Synchronous Machines With Excitation Through Stator Dc Coils Or Magnets And Reluctance Rotors, Oluwaseun A. Badewa Jan 2025

Analysis And Design Optimization Of Synchronous Machines With Excitation Through Stator Dc Coils Or Magnets And Reluctance Rotors, Oluwaseun A. Badewa

Theses and Dissertations--Electrical and Computer Engineering

The design and optimization of electric machines face increasing demands for higher efficiency, improved torque density, manufacturability, and effective utilization of materials, particularly in applications such as electric traction and propulsion, where performance, reliability, and cost are critical. This work explores innovative synchronous machine configurations with reluctance rotors and stator-combined excitation, including permanent magnet and DC-excited topologies, to address these challenges through advanced design methodologies, computational modeling, and experimental validation. This research is relevant to address the growing demand for high-performance electric machines that combine high power density with costeffective manufacturing. The conventional limitations in power and torque density, thermal …


Development Of An Ecg-Based Deep Learning Model For Pediatric Congenital Heart Disease (Chd) Diagnosis, Annbar Mekouar Jan 2025

Development Of An Ecg-Based Deep Learning Model For Pediatric Congenital Heart Disease (Chd) Diagnosis, Annbar Mekouar

Selected Full-Text Master Theses 2021-

Congenital heart disease (CHD) stands as the leading congenital anomaly which affects pediatric populations throughout the world. The effectiveness of treatment depends on both early diagnosis and accurate identification but echocardiography requires manual interpretation which proves time-consuming and inconsistent especially when examining pediatric patients with their distinct cardiac systems. The research aims to create a deep learning-based diagnostic framework which uses ECG data to identify coronary artery disease subtypes in pediatric patients. The model uses high-quality datasets from Dr. Ignacio Lugones to extract R-R intervals and QRS durations through convolutional neural networks (CNNs). The system addresses pediatric-specific challenges while enhancing …


Comparison Of Laboratory Scale Bourbon Whiskey Mashing And Fermentation To Pilot Scale, Ryan C. Sarhan, Brad J. Berron, Czarena L. Crofcheck, Glenna Joyce Welsko, Tyler J. Barzee Jan 2025

Comparison Of Laboratory Scale Bourbon Whiskey Mashing And Fermentation To Pilot Scale, Ryan C. Sarhan, Brad J. Berron, Czarena L. Crofcheck, Glenna Joyce Welsko, Tyler J. Barzee

Biosystems and Agricultural Engineering Faculty Publications

Why the work was done: Laboratory scale methods are useful for optimising grain spirit production. While small scale methods exist, comparisons between conventional, simultaneous saccharification and fermentation, and distillery scale production are lacking. Further, there is limited understanding of how these methods affect the rheological properties of the mash and flavour compounds from fermentation. This study compared conventional and simultaneous saccharification and fermentation mashing methods to a pilot scale distillery to establish a standard laboratory method for simulating industrial bourbon production.

How the work was done: Two mashing methods for bourbon whiskey were compared: simultaneous saccharification and fermentation versus conventional …


Contaminant Mixtures And Their Impact On Nitrate Removal In Wetlands: A Mesocosm Study, Emily N. Byers, Tiffany L. Messer, Daniel N. Miller, Christopher Barton, Jason M. Unrine, Carmen Agouridis Jan 2025

Contaminant Mixtures And Their Impact On Nitrate Removal In Wetlands: A Mesocosm Study, Emily N. Byers, Tiffany L. Messer, Daniel N. Miller, Christopher Barton, Jason M. Unrine, Carmen Agouridis

Biosystems and Agricultural Engineering Faculty Publications

Constructed wetlands are used extensively to mitigate surface runoff. While wetland treatment for nitrogen (N) has been comprehensively studied, a knowledge gap remains regarding the implications of other contaminants (e.g., pesticides, pharmaceuticals) on nitrate-N (NO3-N) removal. This study sought to fill that gap by determining the impact imidacloprid, caffeine, perfluorooctane sulfonic acid (PFOS), atrazine, glyphosate, and sulfate (SO42−) have on NO3-N removal rates. The contaminants were determined based on their occurrence across Kentucky surface waters in urban (imidacloprid, caffeine, and PFOS) and rural (atrazine, glyphosate, and SO42−) environments. Two constructed …


Representation Learning Of Point Cloud Data For Process Mining And Anomaly Detection In Complex Systems, Yujing Yang Jan 2025

Representation Learning Of Point Cloud Data For Process Mining And Anomaly Detection In Complex Systems, Yujing Yang

Industrial, Manufacturing, and Systems Engineering Dissertations - Archive

Complex systems, e.g., advanced manufacturing systems, are largely associated with dynamic and transient behaviors, resulting in condition changes and anomalies. Sensor-based condition monitoring is critical in detecting anomalies and supporting process monitoring and performance improvement for complex manufacturing systems. Traditional sensor-based monitoring approaches primarily focus on one-dimensional (1D) signals and two-dimensional (2D) images, which are limited in their ability to capture high-resolution spatial patterns pertaining to anomalies induced by systems’ condition changes, especially subtle ones. Recent advancements in three-dimensional (3D) sensing present a unique opportunity to address this limitation by enabling the capture of 3D point cloud data with micro-level …


A Pyrrole Modified 3,4-Propylenedioxythiophene Conjugated Polymer As Hole Transport Layer For Efficient And Stable Perovskite Solar Cells, Yuanhao Tang, Ke Ma, Wenhao Shao, Yoon Ho Lee, Ashkan Abtahi, Jiaonan Sun, Hanjun Yang, Aidan H. Coffey, Harindi R. Atapattu, Mustafa Ahmed, Qixuan Hu, Wenzhan Xu, Raunak Dani, Limei Wang, Chenhui Zhu, Kenneth R. Graham, Jianguo Mei, Letian Dou Jan 2025

A Pyrrole Modified 3,4-Propylenedioxythiophene Conjugated Polymer As Hole Transport Layer For Efficient And Stable Perovskite Solar Cells, Yuanhao Tang, Ke Ma, Wenhao Shao, Yoon Ho Lee, Ashkan Abtahi, Jiaonan Sun, Hanjun Yang, Aidan H. Coffey, Harindi R. Atapattu, Mustafa Ahmed, Qixuan Hu, Wenzhan Xu, Raunak Dani, Limei Wang, Chenhui Zhu, Kenneth R. Graham, Jianguo Mei, Letian Dou

Chemical and Materials Engineering Faculty Publications

Despite the outstanding electric properties and cost-effectiveness of poly- (3,4-ethylenedioxythiophene) (PEDOT) and its derivatives, their performance as hole transport layer (HTL) materials in conventional perovskite solar cells (PSCs) has lagged behind that of widely used spirobifluorene-based molecules or poly(triaryl amine). This gap is mainly from their poor solubility and energy alignment mismatch. In this work, the design and synthesis of a pyrrole-modified HTL (PPr) based on 3,4-propylenedioxythiophene (ProDOT) are presented for efficient and stable PSCs. As a result of the superior defects passivation ability, excellent contact with perovskite, enhanced hole extraction, and high hydrophobicity, the unencapsulated PPr-based PSCs showed the …


Analysis Of Tar And Oil Derived From Pyrolysis And Copyrolysis Of Waste Plastics And Biomass, Anitha Shankaralinge Gowda, Dimitrios Karadimas, Jeffrey Seay Jan 2025

Analysis Of Tar And Oil Derived From Pyrolysis And Copyrolysis Of Waste Plastics And Biomass, Anitha Shankaralinge Gowda, Dimitrios Karadimas, Jeffrey Seay

Chemical and Materials Engineering Faculty Publications

Pyrolysis has been proposed as a potential technology for managing the growing volume of plastic waste generated worldwide. Co-pyrolysis of plastic waste with biomass is a promising technology for generating fuel and chemical products. However, this process generates tar as a waste product. The chemical properties of this tar have yet to be thoroughly analyzed. This study presents the results of gas chromatography–mass spectrometry (GC–MS), Fourier-transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA) of oil and tar obtained from the pyrolysis of pure plastics including high-density polyethylene (HDPE), low-density polyethylene (LDPE), polyethylene (PE), polystyrene (PS), and plastic-biomass mixtures. GC–MS analysis …


Membrane Filtration Of Nanoscale Biomaterials: Model System And Membrane Performance Evaluation For Aav2 Viral Vector Clarification And Recovery, Mara Leach, Kearstin Edmonds, Emily Ingram, Rebecca E. Dutch, Ranil Wickramasinghe, Malgorzata Chwatko, Dibakar Bhattacharyya Jan 2025

Membrane Filtration Of Nanoscale Biomaterials: Model System And Membrane Performance Evaluation For Aav2 Viral Vector Clarification And Recovery, Mara Leach, Kearstin Edmonds, Emily Ingram, Rebecca E. Dutch, Ranil Wickramasinghe, Malgorzata Chwatko, Dibakar Bhattacharyya

Chemical and Materials Engineering Faculty Publications

The growing demand for viral vectors as nanoscale therapeutic agents in gene therapy necessitates efficient and scalable purification methods. This study examined the role of nanoscale biomaterials in optimizing viral vector clarification through a model system mimicking real AAV2 crude harvest material. Using lysed HEK293 cells and silica nanoparticles (20 nm) as surrogates for AAV2 crude harvest, we evaluated primary (depth filters) and secondary (membrane-based) filtration processes under different process parameters and solution conditions. These filtration systems were then assessed for their ability to recover nanoscale viral vectors while reducing DNA (without the need for endonuclease treatment), protein, and turbidity. …


Fundamental And Application Of Co-Assembly Of Peptides And Proteins: Experiment And Computation, Newton A. Ihoeghian, Qing Shao Jan 2025

Fundamental And Application Of Co-Assembly Of Peptides And Proteins: Experiment And Computation, Newton A. Ihoeghian, Qing Shao

Chemical and Materials Engineering Faculty Publications

Peptides and proteins can co-assemble into various nanostructures based on complementary non-covalent interactions such as electrostatic forces, hydrogen bonding, and hydrophobic associations. These co-assemblies create a design space of functional materials for a wide spectrum of energy, environmental, and biomedical applications. This review focuses on the fundamentals and applications of three co-assembling systems: ultra-short peptides, peptides, and proteins. We will present and discuss experimental studies demonstrating applications in drug delivery, tissue engineering, and biomaterials development. We will also discuss the contribution of computational research, including molecular dynamics simulations and machine learning, to enhance our understanding of assembly mechanisms. Integrating experimental …


Advances In Magnetic Nanoparticles For Molecular Medicine, Xiaoyue Yang, Sarah E. Kubican, Zhongchao Yi, Sheng Tong Jan 2025

Advances In Magnetic Nanoparticles For Molecular Medicine, Xiaoyue Yang, Sarah E. Kubican, Zhongchao Yi, Sheng Tong

Biomedical Engineering Faculty Publications

Magnetic nanoparticles (MNPs) are highly versatile nanomaterials in nanomedicine, owing to their diverse magnetic properties, which can be tailored through variations in size, shape, composition, and exposure to inductive magnetic fields. Over four decades of research have led to the clinical approval or ongoing trials of several MNP formulations, fueling continued innovation. Beyond traditional applications in drug delivery, imaging, and cancer hyperthermia, MNPs have increasingly advanced into molecular medicine. Under external magnetic fields, MNPs can generate mechano- or thermal stimuli to modulate individual molecules or cells deep within tissue, offering precise, remote control of biological processes at cellular and molecular …


Clinical Correlation Of Sars-Cov-2 Wastewater Passive Sampling In Long-Term Care Facilities And Wastewater Treatment Plants, William Strike, Alexus Lori Rockward, Blazan Mijatovic, Ann Noble, Cullen Olsson, Soroosh Torabi, Mohammad Dehghan Banadaki, Reuben Adatorwovor, James W. Keck, Scott M. Berry Jan 2025

Clinical Correlation Of Sars-Cov-2 Wastewater Passive Sampling In Long-Term Care Facilities And Wastewater Treatment Plants, William Strike, Alexus Lori Rockward, Blazan Mijatovic, Ann Noble, Cullen Olsson, Soroosh Torabi, Mohammad Dehghan Banadaki, Reuben Adatorwovor, James W. Keck, Scott M. Berry

Biomedical Engineering Faculty Publications

Wastewater-based epidemiology (WBE) is a promising tool for improving health outcomes through early detection and cost-effective pathogen surveillance. Long-term care facilities (LTCFs) serve and employ vulnerable populations that may particularly benefit from the use of WBE, but financial and technical costs associated with standard sampling methods limit the feasibility of WBE in the LTCF setting. In this work, we used passive sampling to simplify the wastewater analysis process and compared its performance to the standard composite sampling method. Moore swabs and automatic composite samplers were used concurrently to sample wastewater from two LTCFs, and samples were analyzed for SARS-CoV-2 concentration. …


Affordable Miniaturized Speckle Contrast Diffuse Correlation Tomography Device For Depth-Sensitive Mapping Of Cerebral Blood Flow In Rodents, Fatemeh Hamedi, Faezeh Akbari, Mehrana Mohtasebi, Chong Huang, Li Chen, Lei Chen, Guoqiang Yu Jan 2025

Affordable Miniaturized Speckle Contrast Diffuse Correlation Tomography Device For Depth-Sensitive Mapping Of Cerebral Blood Flow In Rodents, Fatemeh Hamedi, Faezeh Akbari, Mehrana Mohtasebi, Chong Huang, Li Chen, Lei Chen, Guoqiang Yu

Biomedical Engineering Faculty Publications

Significance: Continuous and longitudinal monitoring of cerebral blood flow (CBF) is critical for understanding brain pathophysiology and guiding interventions. Although rodents are the primary models in neuroscience, existing imaging modalities often fail to provide the optimal combination of low cost, high spatiotemporal resolution, wide head coverage, and sufficient penetration depth for small-animal brain imaging.

Aim: Leveraging a clinical speckle contrast diffuse correlation tomography (scDCT) system, we aimed to develop an affordable, user-friendly, fast, and miniaturized scDCT (mini-scDCT) device tailored for depth-sensitive CBF imaging in small rodents.

Approach: The mini-scDCT replaces bulky and costly optoelectronic components with compact, low-cost alternatives while …


Implementing Wastewater-Based Epidemiology For Long-Read Metagenomic Sequencing Of Antimicrobial Resistance In Kampala, Uganda, William Strike, Temitope O. C. Faleye, Brian Lubega, Alexus Lori Rockward, Soroosh Torabi Jan 2025

Implementing Wastewater-Based Epidemiology For Long-Read Metagenomic Sequencing Of Antimicrobial Resistance In Kampala, Uganda, William Strike, Temitope O. C. Faleye, Brian Lubega, Alexus Lori Rockward, Soroosh Torabi

Biomedical Engineering Faculty Publications

Antimicrobial resistance (AMR) is an emerging global threat that is expanding in many areas of the world. Wastewater-based epidemiology (WBE) is uniquely suited for use in areas of the world where clinical surveillance is limited or logistically slow to identify emerging threats, such as in Sub-Saharan Africa (SSA). Wastewater was analyzed from three urban areas of Kampala, including a local HIV research clinic and two informal settlements. Wastewater extraction was performed using a low-cost, magnetic bead-based protocol that minimizes consumable plastic consumption followed by sequencing on the Oxford Nanopore Technology MinION platform. The majority of the analysis was performed using …