Biotechnology Commons^™

Cellular Mechanism Of Pentagalloyl Gucose-Mediated Prevention And Reversal Of Abdominal Aortic Aneurysms, Greg Halsey

All Dissertations

Abdominal aortic aneurysm (AAA) has been the 15th leading cause of death in persons older than 55 for the past ten years in several Western countries, with rupture resulting in an almost 100% mortality rate. AAA is a focal dilation of the aorta characterized by progressive loss of elastin and vascular smooth muscle cells (SMCs), as well as persistent inflammatory response [1]. The only treatment for AAA is surgery; however, the mortality post-procedure is still 1-4%, with a 15-30% chance of morbidity [2].

Pentagalloyl glucose (PGG) treatment is effective in the reversal of AAA. While its stabilizing interactions with extracellular …

A Robust Agroinfiltration Method, Bryce N. Trull

Masters Theses

Stable transformation of soybean (Glycine max) is a markedly slow and laborious process. Thus, a tool that enables rapid evaluation of genetic elements in planta is critical to advance complex research and genetic engineering in soybean. To that end, a substantially robust agroinfiltration method was innovated in this work. Agroinfiltration is a technique that leverages Agrobacterium-mediated transient transformation to deliver genetic elements to the cells of whole plant tissues, usually leaves. Several factors were found to be relevant to successful soybean leaf agroinfiltration, including genotype, surfactant, developmental stage, and Agrobacterium culture medium. The research represents not only …

Protective Responses Of The Intestinal Epithelial Cell Line Ht-29 Cells Exposed To Dephosphorylated Salmonella Flagellin [Research Note], Heyeon Baik, Jaiesoon Cho

The Philippine Agricultural Scientist

This study aimed to describe the effects of Salmonella Typhimurium flagellin (SFL) dephosphorylated by sweet potato purple acid phosphatase (PAP) on the protective responses of the intestinal epithelial cell line HT-29 cells. The enzyme was reported to display a broad substrate specificity for various organic phosphorylated conjugates and phosphoproteins. Dephosphorylation of SFL by sweet potato PAP decreased to 35% in the presence of 0.05 mM vanadate as compared with the negative control (p < 0.05). Intact SFL and the SFL treated with sweet potato PAP did not remarkably induce the activation of caspase-3 in HT-29 cells at all the tested levels of the substrate. Intact SFL maximally induced the release of IL (interleukin)-8 in HT-29 cells at 1000 ng/mL (p < 0.05). However, the SFL treated with the enzyme inhibited the release of IL-8 at over 100 ng/mL of the substrate as compared with intact SFL, resulting in an approximately 8-fold decrease even at 1000 ng/mL (p < 0.05). The SFL treated with the enzyme decreased the activation of the total ERK1/2 in the cells to 1.9 and 1.7–fold at 10 and 1000 ng/mL of the substrate, respectively, as compared with intact SFL (p < 0.05). In conclusion, sweet potato PAP could be a promising tool for controlling excessive inflammation during Salmonella infection in animal husbandry, and the enzyme could be a safe alternative that can overcome the drawbacks of chemotherapy.

Village-Level Processing Of Pangi (Pangium Edule Reinw.) Seeds By The Obu Manuvu Improves The Edibility And Level Of Some Nutritional Factors [Research Note], Rovi Gem E. Villame-Gayagas, Jennifer P. Fronteras, Maria Christna D. Nacar, Aaron P. Lorilla, Pedro A. Alviola Iv

The Philippine Agricultural Scientist

Pangi (Pangium edule Reinw) seed is known to be a poisonous material. However, it becomes safe for consumption after processing. This study determined the effect of village-level processing by the Obu Manuvu community of Marilog District, Davao City, Philippines on the antinutrient and nutritional composition of Pangi seeds. Processing methods involved washing with running water for about 15 s, boiling in water for 30 min, and soaking in flowing water in a nearby river overnight. Results showed that after processing, the levels of antinutrients such as cyanogenic glycosides and oxalates were reduced significantly (p < 0.05) by 99.15% and 78.23%, respectively. Further, the tannin content was also significantly reduced by 96.15%. The crude fat content significantly increased (p < 0.05) by about 54%. Crude ash and fiber decreased, while protein did not change significantly (p < 0.05). Minerals such as calcium and zinc increased (p < 0.05) by 221.43% and 64.39%, respectively while the iron and manganese levels remained unaffected. Findings of the study suggested that the processing method by the Obu Manuvu effectively reduces the level of antinutrients, thereby improving the edibility and safety of Pangi seeds. Further, the processing method improves the level of some nutrients, specifically crude fat, calcium, and zinc.

Exploring The Impact Of High-Temperature Stress On Physiological, Anatomical, Molecular, Productive, And Quality Parameters In Diverse Olive Cultivars, Bassam F. Alowaiesh, Taghreed S. Alnusaire

The Philippine Agricultural Scientist

Olive (Olea europaea L.) is one of the most important horticultural crops in Saudi Arabia. High temperatures are typical in most regions during the summer season. This study explored the effect of high temperatures on diverse olive cultivars (Manzanillo, Chemlali, and Picual) grown in three different geographical locations in Saudi Arabia (Sakaka, Basita, and Al-Qurayyat). Sakaka region was classified as a hot region, while the Basita and Al-Qurayyat regions were characterized by relatively mild or low temperatures. Genotypic components were assessed by studying Amplified Fragment Length Polymorphism (AFLP) molecular markers and physiological, anatomical, productive, and oil quality parameters. The …

Exploring The Nutritional, Antioxidant, And Lipid-Lowering Properties Of Saba Banana (Musa Acuminata X Balbisiana Bbb Group) Peels, Paul Alteo Bagabaldo, Liezl M. Atienza, Katherine Ann T. Castillo-Israel, Aimee Sheree A. Barrion, Antonio C. Laurena, Maria Amelita C. Estacio

The Philippine Agricultural Scientist

This study investigated the nutritional and functional components of ‘saba’ banana peel (SBP) to determine its health-promoting potential for special food applications. SBP has undergone analyses measuring proximate composition, vitamin C, antioxidant activity, phytochemical content, estimated glycemic index (eGI), pancreatic lipase inhibition, and cholesterol micellar solubility inhibition. Data were processed using SPSS v20 and expressed as mean ± SD. Proximate analysis showed that SBP contained 83.83 ± 1.06% moisture, 6.74 ± 0.83% crude fat, 5.58 ± 0.24% crude protein, 9.97 ± 0.04% total ash, 9.91 ± 0.10% crude fiber, and 60.08% nitrogen-free extracts. Vitamin C content was found to be …

A Review Of Threat Vectors To Dna Sequencing Pipelines, Tyler Rector

Cybersecurity Undergraduate Research Showcase

Bioinformatics is a steadily growing field that focuses on the intersection of biology with computer science. Tools and techniques developed within this field are quickly becoming fixtures in genomics, forensics, epidemiology, and bioengineering. The development and analysis of DNA sequencing and synthesis have enabled this significant rise in demand for bioinformatic tools. Notwithstanding, these bioinformatic tools have developed in a research context free of significant cybersecurity threats. With the significant growth of the field and the commercialization of genetic information, this is no longer the case. This paper examines the bioinformatic landscape through reviewing the biological and cybersecurity threats within …

Controlled Radiation Capsule For Precision And Rapid Cancer Treatment, Hoseon Lee, Zsolt Kollar, Bailey R. White, Junia Nguyen, David Roque, Sowjanya Palagani

Symposium of Student Scholars

This research aims to transform cancer treatment through the optimization of brachytherapy, with a focus on reducing treatment duration, setup complexities, and financial burdens, all while emphasizing patient safety. Patients living at a distance from radiation clinics, particularly those undergoing extended Low Dose Radiation brachytherapy, often struggle with the formidable financial challenges associated with securing nearby accommodations. In response to these issues, the research introduces a radiation capsule designed to condense the conventional six-month treatment period to approximately just one week, thereby significantly reducing the duration of required accommodations. This capsule is especially relevant considering the construction cost of $40 …

Evaluation Of Crocus Sativus L. Quality Using Ftir Spectroscopy, Lina M. Alnaddaf, Mohamad Nabil Ksaier, Salim F. Bamsaoud

Hadhramout University Journal of Natural & Applied Sciences

Crocus sativus L. is one the important spices that gives food a distinctive color and taste as well as its medicinal value. Its quality can be estimated using different analysis methods. In This study we used FTIR spectroscopy to Evaluate Crocus sativus L. quality at MEDICO Lab 2023. FTIR Spectroscopy is a fast and reliable analytical method for quality saffron evaluation. The results of FTIR spectra indicated functional groups related to O-H, C=C, C=O, C-O, C(O)–O, C-H, C-H (cis-) and C-H (trans-). Also, our samples lacked the distinctive bands of the active substances of crocetin. Furthermore, …

Fine Time Synchronization Algorithm For Mimo-Ofdm, Ajstd Editorialboard

ASEAN Journal on Science and Technology for Development

No abstract provided.

Steady State Finite Element Analysis Of A Single Sttack Cold Plate With Heat Losses, G. A Quadir, Shiao Lin Beh, Kn Seetharamu, Ay Hassan

ASEAN Journal on Science and Technology for Development

No abstract provided.

Mitigating Co2 Emissions From The Power Sector:Least-Cost Analysis Of Policy Option In Thailand, Bundit Limmeechokchai, Somporn Tanatvanit, Ram M. Sherestha

ASEAN Journal on Science and Technology for Development

No abstract provided.

Study Of Preparation And Charaterization Of Hydroxyapatite Coating By Sol-Gel Technique, Sutapa Roy, Ramanan Venkatesh

ASEAN Journal on Science and Technology for Development

No abstract provided.

Thermal Perfomarmance Prediction Of Plastics Ball Grid Array (Pbga) Using Artificial Neural Network, C H Leong, I A Aziz, K N Seetharamu

ASEAN Journal on Science and Technology for Development

No abstract provided.

Analysis Of Quality Cost For Statistical Quality Control Planning, N Chiadamrong, C Thaviwatanachaikul

ASEAN Journal on Science and Technology for Development

No abstract provided.

Impreved Stability Evaluation And Blast Planning In Quaries, Sanga Tangehawal

ASEAN Journal on Science and Technology for Development

No abstract provided.

Effects Of Thinning On The Incidence Of Damage And Severity Of Decay In Bagras (Eucalyptus Deglupta Blume) Plantations, Emelio Anino

ASEAN Journal on Science and Technology for Development

Thinning of intermediate and suppressed trees has been implemented on 3- to 4-year-old bagras (E. deglupta) plantations in Surigao de! Sur, Philippines. The objective is to remove unwanted competition and to gain some form of revenue by selling the felled trees as banana props. However, the falling trees caused damage to other plantation trees. Bagras is susceptible to heart rot. A study was, therefore, conducted to determine the extent of decay in bagras due to thinning. Data showed that injured bagras developed extensive decay in the sapwood and heartwood. Six percent of the injured trees died. Retained-trees, which were already …

Seasonal Orientation Of Solar Collectors For The Philippines, Saiful Bari

ASEAN Journal on Science and Technology for Development

In this paper, a method to determine the slope angle for seasonal applications of flat plate collectors is described for the Philippines territory. For seasonal applications, where the collectors are used only for a fraction of the year, the slope angles need to be different from those for year-round operations. For year-round operations, most flat collectors are usually fixed, and mounted at an angle equal to the latitude tilt angle. The method described here utilized both direct and diffuse components of solar radiation, and air mass using average atmosphere. For seasonal applications of solar energy such as crop drying, the …

Thermal Diffusivity Measurements Using Open Photoacoustic Cell Technique, W. Mahmood Mat Yunus, C. Y. J. Fanny, I. V. Grozesu, S. A. Halim, M. M. Moksin

ASEAN Journal on Science and Technology for Development

The application of open photoacoustic cell (OPC) technique is demonstrated for measuring thermal diffusivity of solid samples. It is based upon the measurement of the photoacoustic signal in the modulation frequency region where the thermal diffusion length equals to the sample thickness. The measurements were carried out at room temperature for gold alloys (Au0 75Ag0.and Au. Ag. ); epoxidised natural rubbers 25 0 50 0 50 [ENR 25 (75%C ) and ENR 50 (75%C)]; and supercond ucti ng ceramics (Bi2Pb0 .6 Sr2Ca1.94Ba 0. Cu 0 , and Bi Pb . Sr Ca1. Zn. Cu 0 ) . The thermal 06 …

An Experiment With Spiral Wound Reverse Osmosis Membranes For The Desalination Of Seawater, M.N.A. Hawlader, J.C. Ho, K. T. Chua

ASEAN Journal on Science and Technology for Development

In the research, the performance characteristics of Reverse Osmosis (RO) Spiral Wound (SW) membrane are evaluated. The effects of feed water concentration, temperature, pressure and flow rate on the performance of this membrane are investigated. The product recovery () of SW membrane is found to increase with feed water temperature and pressure, but decrease with increasing feed water concentration and flow rate. Salt passage (SP) increases with feed water temperature and concentration, but decreases with increasing feed pressure and flow rate. Under the tested feed water conditions, of SW varies from 6% - 18% and permeate salinity is approximately …