Characterizing The Role Of Pa5189 Of Pseudomonas Aeruginosa In Deletion And Overexpression Mutants, 2024 University of Nebraska at Omaha
Characterizing The Role Of Pa5189 Of Pseudomonas Aeruginosa In Deletion And Overexpression Mutants, Seh Na Mellick
Theses/Capstones/Creative Projects
In the context of rising multidrug resistance in biofilm-forming pathogens like Pseudomonas aeruginosa, this study investigates the role of the understudied transcription factor PA5189 in antibiotic resistance and biofilm formation. PA5189 deletion and overexpression mutants were created in a parent P. aeruginosa strain using pEX18Tc-based recombinant suicide vectors, with genotypic verification of putative triparental conjugants achieved through restriction digestion and PCR. The study revealed that PA5189 overexpression significantly increases resistance to commonly used broad spectrum antibiotics such as ciprofloxacin and imipenem. Additionally, differential expression of PA5189 was found to notably affect biofilm formation, with variations contingent on the nutrient …
Determining The Impacts Of Corn Silage Mixing Ratios On Hydrogen Production From Beef Cattle Manure, 2024 University of Nebraska-Lincoln
Determining The Impacts Of Corn Silage Mixing Ratios On Hydrogen Production From Beef Cattle Manure, Katya Faber-Quimby
Honors Theses
With global warming becoming an increasingly serious threat and a growing population requiring additional energy resources, interest in the production of clean energy from the agricultural sector has surged in popularity. Biohydrogen, also known as green hydrogen, is H2 that has been produced using renewable energy, such as from agricultural byproducts or waste materials. It presents a promising alternative to nonrenewable resources due to its high potential for energy storage and lack of carbon emissions when used. Cattle manure has been explored as a resource for the production of biohydrogen. Selecting for hydrogen-producing microbes in the manure serves the …
Cultivating Excellence: A Literature Review On Harnessing The Power Of The Gut Microbiome For Athletic Performance, 2024 Bowling Green State University
Cultivating Excellence: A Literature Review On Harnessing The Power Of The Gut Microbiome For Athletic Performance, Maya Katharine Dean
Honors Projects
The interplay between our gut microbiome and health is immense. This literature review analyzes the current research assessing the interplay between gut microbiome and athletic performance. Knowing how to improve gut microbial diversity via nutrition and supplementation can take athletic performance to the next level; namely improvements in immune, mental, and physical health.
Hgs-3 The Influence Of A Tandem Cycling Program In The Community On Physical And Functional Health, Therapeutic Bonds, And Quality Of Life For Individuals And Care Partners Coping With Parkinson’S Disease, 2024 university of south carolina school of medicine greenville
Hgs-3 The Influence Of A Tandem Cycling Program In The Community On Physical And Functional Health, Therapeutic Bonds, And Quality Of Life For Individuals And Care Partners Coping With Parkinson’S Disease, Leila Djerdjour, Jennifer L. Trilk
SC Upstate Research Symposium
Purpose Statement: Several studies have shown that aerobic exercise can have a positive impact on alleviating symptoms experienced by individuals with Parkinson's disease (PD). Despite this evidence, the potential benefits of exercise for both PD patients and their care partners (PD dyad) remain unexplored. This research project investigates the effectiveness, therapeutic collaborations, and physical outcomes of a virtual reality (VR) tandem cycling program specifically designed for PD dyads.
Methods: Following approval from the Prisma Health Institutional Review Board, individuals with PD were identified and screened by clinical neurologists. The pre-testing measures for PD dyads (N=9) included emotional and cognitive status …
Adherent-Invasive Escherichia Coli (Aiec) Promotes Mild Colitis In The C57bl/6 Il-10 Ko Mouse Model Of Crohn's Disease, 2024 University of South Carolina - Columbia
Adherent-Invasive Escherichia Coli (Aiec) Promotes Mild Colitis In The C57bl/6 Il-10 Ko Mouse Model Of Crohn's Disease, Sarah Sm Zaw
Senior Theses
Crohn's disease (CD) is a chronic inflammatory bowel disease. Interleukin-10 knockout (IL-10 KO) mice are crucial in CD research. Adherent-invasive Escherichia coli (AIEC) is a subgroup of E. coli. In one study, IL-10 KO mice colonized with AIEC developed colitis in the distal colon compared to wild-type (WT) mice. I investigated how AIEC NC101 WT affects colitis severity in IL-10 KO mice of the C57BL/6 genetic background. Similar results in both genotypes would enhance mouse strain accessibility. Germ-free C57BL6 IL-10 KO mice were divided into groups: colonized with AIEC NC101 for 12, 15, or 18 weeks, or kept germ-free. Histopathological …
A Review On Bioremediation Of Heavy Metals And Hydrocarbons Through Plant Growth-Promoting Bacteria And Composting, 2024 Department of Bioinformatics and Biosciences, Capital University of Science and Technology, Islamabad, Pakistan
A Review On Bioremediation Of Heavy Metals And Hydrocarbons Through Plant Growth-Promoting Bacteria And Composting, Hafsa Saleem, Hadia Farooq, Rimsha Mazhar, Sadaf Shakil, Sahar Fazal
Journal of Bioresource Management
Soil is an important ecosystem component; it serves as a habitat for many microscopic organisms that play an important role in the ecosystem's maintenance and crops grown in soil. Unfortunately, human activities have adversely affected not only the environment but also the soil health. Soil has been contaminated with heavy metals hydrocarbons and hydrocarbon-based products that are affecting soil health and the growth of plants. In this review two methods of heavy metal remediation are discussed; one is the role of plant growth-promoting bacteria (PGPB) in the enhancement of phytoremediation and the other is composting. PGPB help plants to grow …
An Antiviral Response Unleashed: Deciphering The Relationship Between Shiftless And Rna Granules During Kshv Infection, 2024 University of Massachusetts Amherst
An Antiviral Response Unleashed: Deciphering The Relationship Between Shiftless And Rna Granules During Kshv Infection, David C. Hatfield
Masters Theses
Herpesviruses persist as a parasitic actor among many species. These viral agents can rapidly seize control over host cells by influencing global gene expression. Through a process known as host shutoff, herpesviruses cause a widespread degradation event of host transcripts within the cytoplasm. Specifically, Kaposi Sarcoma associated herpesvirus (KSHV) encodes for an endoribonuclease, termed SOX, that orchestrates this manipulation of gene expression. We and others have discovered certain transcripts that escape this fate; we suggest that this is an active escape, where transcripts have 3’ UTR elements that disallow SOX cleavage. One of the escapees that has been found is …
Synthesis, Molecular Characteristics, And Antibacterial Assessment Of Marine Hydroid Aqueous Extract-Based Silver Bio-Nanoparticles, 2024 Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Hasanuddin, Sulawesi Selatan 90245, Indonesia
Synthesis, Molecular Characteristics, And Antibacterial Assessment Of Marine Hydroid Aqueous Extract-Based Silver Bio-Nanoparticles, Fredryk Mandey, Aulia Rhamdani Arfan, Rugaiyah Andi Arfah
Makara Journal of Science
This investigation aims to synthesize, analyze the molecularity, and test the ability of bacterial inhibition capability of silver nanoparticles that have been synthesized by simply mixing silver nitrate and aqueous extracts of marine natural products with and without the addition of amylum as a stabilizing agent. This research, with and without the addition of amylum as a stabilizing agent, obtained 39.0 and 55.2 mg of solids of round-shaped morphology silver nanoparticles with diameters of 87.9 and 103.0 nm., respectively. In addition, the antibacterial testing assay against Staphylococcus aureus and Escherichia coli showed some considerably good results. S. aureus with the …
Molecular Detection Of Human And Dog Fecal Pollution In Pensacola, Florida, 2024 Georgia College & State University
Molecular Detection Of Human And Dog Fecal Pollution In Pensacola, Florida, Logan Mccullers, Jodel Nicholas, Dave Bachoon
Graduate Research Showcase
Pensacola, Florida (USA), has a thriving coastal community that depends on its coastal areas for recreation, shellfish harvesting, and fisheries. However, increasing levels of fecal pollution pose a potential threat to the water quality of these coastal systems. Identifying the source and abundance of contaminants in these environments is critical to controlling fecal pollution in these areas. This study aimed to use Microbial Source Tracking techniques to determine the source of pollution in five sites (Bayou Texar Lower, Willard Norris Road Creek, Clear Creek Boat Ramp @ 87, Quinette River Boat Ramp and Pensacola Bay Bridge) in the Pensacola area. …
Vitro Bacteriophage Treatment For A Staphylococcus Aureus And Pseudomonas Aeruginosa Co-Culture, 2024 Ouachita Baptist University
Vitro Bacteriophage Treatment For A Staphylococcus Aureus And Pseudomonas Aeruginosa Co-Culture, Alexis Perry
Arkansas Women in STEM Conference
Recent studies show that up 27-36% of all infections are polymicrobial, involving more than one bacteria species. In all categories, polymicrobial infections are often more virulent and harmful to the patient. Since different types of bacteria can communicate with each other through quorum-sensing when in the presence of each other, they can display different genomic characteristics. Further research into polymicrobial infections needs to be done because these changing characteristics can prevent treatment from working, such as antibiotics or bacteriophage therapy. This experiment focused on the co-culture between the two bacteria, Pseudomonas aeruginosa and Staphylococcus aureus, which can be found co-existing …
Investigating The Escape Mechanism Of Sre Bearing Mrna Transcripts During Viral Host Shutoff, 2024 University of Massachusetts Amherst
Investigating The Escape Mechanism Of Sre Bearing Mrna Transcripts During Viral Host Shutoff, Daniel Macveigh-Fierro
Doctoral Dissertations
During viral infection, the virus and host clash for control over gene expression in an evolutionarily arms race that has raged for thousands of years. During lytic replication, Kaposi’s sarcoma associated herpesvirus (KSHV) triggers a massive RNA decay event known as host shut off. This causes over 70% of all RNA to be degraded suppressing the host antiviral response while freeing resources for viral replication. Our lab focuses on a subset of transcripts that escape from this viral degradation event using a cis acting 3’ UTR element known as a “SOX resistant element” or SRE. Although we have identified a …
The Impacts Of Environment And Host Evolutionary Relationships On Lemur Microbiota, 2024 University of Massachusetts Amherst
The Impacts Of Environment And Host Evolutionary Relationships On Lemur Microbiota, Rachel B. Burten
Doctoral Dissertations
Recent studies have shown that the mammal microbiome is modified by environmental conditions, and that reduced microbiome functionality is associated with host health issues. Microbiome data in wild and captive primate populations can therefore be used to assess their health as they encounter a variety of environments. Comparative studies of the microbiome can also inform disease ecology, conservation, and captive management strategies tailored to different primate species. Therefore, this study examines how the hair, oral, and gut microbiota of nine wild and captive lemur species are determined by host phylogenetic relationships and host environment. I found that host species identity …
Targeting Mycobacterial Efflux System For Combating Anti-Microbial Resistance, 2024 Amity University Uttar Pradesh
Targeting Mycobacterial Efflux System For Combating Anti-Microbial Resistance, Arathi Radhakrishnan, Raj Kishor Kapardar, Rajpal Srivastav
Research Symposium
Background: The drug resistance in the microbes is a serious concern in medicine. Along with intrinsic factors, extrinsic factors like unprescribed usage of drugs are the contributing factors. The drug tolerance has led to the emergence of superbugs. Mycobacterial species utilize an array of multidrug efflux mechanisms linked to intrinsic and acquired antibiotic resistance. Understanding molecular mechanisms regulating efflux could reveal new therapeutic targets and strategies. Our study is aimed to target regulators of efflux Mycobacterial transporter.
Methods: Using the reference mycobacterial strain, antibiotic sensitivity was first profiled by minimal inhibitory concentration assays across a panel of antimicrobials, followed by …
Materials And Methods Developed For The Recording And Analysis Of Behavior In The Common Marmoset (Callithrix Jacchus)., 2024 University of Nebraska at Omaha
Materials And Methods Developed For The Recording And Analysis Of Behavior In The Common Marmoset (Callithrix Jacchus)., Christian Wintle
UNO Student Research and Creative Activity Fair
Materials and methods developed for the recording and analysis of behavior in the common marmoset (Callithrix jacchus).
Christian J. Wintlea, Jordan B. Hernandeza,b,c, Dobromir Dotovd, and Jonathan B. Claytona,b,e,f,g
aDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA
bNebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA
cDepartment of Genetics, Cell Biology, and Anatomy, University of Nebraska Medical Center, NE, USA
dDepartment of Biomechanics, University of Nebraska at Omaha, Omaha, NE, USA
eDepartment of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, …
The Changing Tumor Microenvironment Of Non-Small Cell Lung Cancer, 2024 Brigham Young University - Provo
The Changing Tumor Microenvironment Of Non-Small Cell Lung Cancer, Mackenzie Hansen, Kim O'Neill, Qin Zhou, Yoko Derose, Magdalena Kovacsovics, Benjamin Brintz, Benjamin L. Witt, Siwen Hu-Lieskovan
Library/Life Sciences Undergraduate Poster Competition 2024
Lung cancer is the leading cause of cancer related death in the United States, and non-small cell (NSCLC) is the most common type. An increasingly prevalent treatment in the last few years for NSCLC has been immunotherapy. However, for immunotherapy to be the most effective, we need to have a better understanding of how the immune system interacts with cancer in all stages. Specifically, we need to know where the immune cells reside, how they interact with each other, and how these populations change as the disease progresses. To define the tumor microenvironment, we stained 27 whole tumor slides with …
Targeting Hsp90: A Novel Approach To Combat Synovial Sarcomas, 2024 Brigham Young University - Provo
Targeting Hsp90: A Novel Approach To Combat Synovial Sarcomas, Peyton Worley, Jared Barrott, Matt Kirkham, Drake Heithoff
Library/Life Sciences Undergraduate Poster Competition 2024
Synovial sarcomas are rare muscle cancers that have very few targeted therapies. HSP90 is a protein upregulated in this form of cancer and many others. A screening of this cancer was done in vitro, using FDA-approved drugs, showing that HSP90 could be a promising treatment option. A novel HSP90 inhibitor was applied to human and mouse synovial sarcoma cell lines and was analyzed after receiving a drug treatment for HSP90.
Carbon-Infiltrated Carbon Nanotube Surfaces Affect Pseudomonas Aeruginosa Biofilm Development, 2024 Brigham Young University - Provo
Carbon-Infiltrated Carbon Nanotube Surfaces Affect Pseudomonas Aeruginosa Biofilm Development, Jenny Finlinson, Lucy C. Bowden, Bradford K. Berges
Library/Life Sciences Undergraduate Poster Competition 2024
• ~30% of surgical implant failure is caused by infection1
• 10-23% of all implant-associated bone and joint infections are caused by gram-negative bacteria2
• Pseudomonas aeruginosa, a gram-negative bacterium, forms biofilms (bacterial communities encased in a matrix) that are extremely difficult to treat
Novel Microbiological Medium Developed For The Isolation Of Bacteria Associated With Estuarine Anemones, 2024 California State Polytechnic University, Humboldt
Novel Microbiological Medium Developed For The Isolation Of Bacteria Associated With Estuarine Anemones, Parker K. Lund, Catalina Cuellar-Gempeler
IdeaFest: Interdisciplinary Journal of Creative Works and Research from Cal Poly Humboldt
Out of the nearly one trillion species of microbiota estimated to inhabit Earth only ten thousand have been cultured in the laboratory. Culturing continues to play a vital role in determining the physiology and ecologic function of individual bacteria in microbial communities and for microbes associated with host organisms one of the major challenges is developing microbiological media that mimics the bacteria’s natural environment enough to promote growth. Here, we target bacteria associated with the estuary anemones Diadumene lineata and Metridium senile by developing a novel medium that uses anemone tissue as the sole source of nutrients. We further measured …
Investigating The Mechanisms Of Surface Sensing Using Motility Appendages By Pseudomonas Aeruginosa Pa14, 2024 Dartmouth College
Investigating The Mechanisms Of Surface Sensing Using Motility Appendages By Pseudomonas Aeruginosa Pa14, Christopher James Geiger
Dartmouth College Ph.D Dissertations
Biofilms are surfaced attached communities of cells encased in an extracellular matrix. The transition from free-swimming planktonic cells to a surface attached biofilm begins with cellular changes that occur after surface contact. This process is known as "surface sensing" and the opportunistic pathogen Pseudomonas aeruginosa PA14 uses its two motility appendages, type IV pili (T4P) and a single, polar flagellum to sense and traverse surfaces. The first cellular changes to occur within this organism upon surface contact is an increase in the second messengers cAMP and cdi- GMP. While the genes involved in surface sensing by P. aeruginosa are known, …
Knowledge, Attitude, And Practice Regarding Deep Dentinal Caries Removal Before Root Canal Treatment And Restoration Among Practicing Dental Surgeons, 2024 Department of Prosthodontics and Crown & Bridge, Institute of Dental Sciences, Siksha ‘O’ Anusandhan (Deemed to be) University, Bhubaneswar, Odisha, India
Knowledge, Attitude, And Practice Regarding Deep Dentinal Caries Removal Before Root Canal Treatment And Restoration Among Practicing Dental Surgeons, Sangram Panda, Anamika Sinha, Shakti Rath, Kajal Kiran Sahoo
Palestinian Medical and Pharmaceutical Journal
This article aims to determine the knowledge, attitude, and practice regarding Deep Dentinal Caries Removal before Root Canal Treatment (RCT) and Restoration among practicing dental surgeons. A descriptive cross-sectional study was executed amongst dental surgeons practicing with undergraduate and post-graduate degrees. A Google questionnaire form was developed and distributed, and 111 authentic entries were short-listed. Data obtained from the study were recorded in Microsoft Excel 2007 version, and SPSS version 18.0 was used for statistical analysis. Most dental surgeons who participated in the study practiced for over 15-20 years. Among them, 60% believe in removing dentinal caries before RCT; 83.8% …