Biology Commons^™

Restoring Polyhyrdoxybutyrate (Phb) Depolymerase Expression In A Bald Mutant Of Streptomyces Sp. Sfb5a, Youmna Moawad

Honors Projects

Polyhydroxybutyrate (PHB) is a biodegradable, plastic-like polymer produced by some bacteria and degraded by others. The filamentous bacterium Streptomyces sp. SFB5A degrades PHB for growth using an extracellular PHB depolymerase, encoded by the phaZ gene. A morphological mutant of this bacterium, bld4, cannot form aerial filaments and cannot produce PHB depolymerase, despite having the phaZ gene. The inability to synthesize PHB depolymerase may be due to a mutation in a gene encoding one of its transcriptional regulators. A gene (lrp) coding for a potential transcriptional regulator is located 2,700 base pairs upstream from phaZ. Our goal was to clone ...

Stream Sediment Monitoring In Morehead Kentucky, Kristopher Krolikowski, Jen O'Keefe

2021 Celebration of Student Scholarship - Oral Presentations

Sediment loads in stream systems play a big role in how overall runoff behaves. Streams naturally transport sediment at various ratios depending on precipitation. When vegetation is removed in upstream environments, sediment loads on streams increase. Sudden increases of sediments in streams can cause many issues, such as flooding or stream re-direction. In Morehead, KY, large-scale logging operations on the ridge above Knapp Avenue and on the ridge above the north end of Allen Avenue have caused increased sediment load in a small stream called Oxley Branch. This stream flows south parallel to Knapp Avenue and along the eastern property ...

Effect Of Nitrogen Addition On Selection Of Germination Trait In An Alpine Meadow On The Tibet Plateau, Kun Liu, Yang Liu, Zhilong Zhang, Shiting Zhang, Carol C. Baskin, Jerry M. Baskin, Ting Liang, Haiyan Bu, Shuxia Li, Tingting Zhang, Xianliang Cui, Sa Xiao

Biology Faculty Publications

Seed germination requirements may determine the kinds of habitat in which plants can survive. We tested the hypothesis that nitrogen (N) addition can change seed germination trait-environmental filter interactions and ultimately redistribute seed germination traits in alpine meadows. We determined the role of N addition on germination trait selection in an alpine meadow after N addition by combining a 3-year N addition experiment in an alpine meadow and laboratory germination experiments. At the species level, germination percentage, germination rate (speed) and breadth of temperature niche for germination (BTN) were positively related to survival of a species in the fertilized community ...

The Spread Of Batrachochytrium Dendrobatidis And Its Effect On The Global Amphibian Ecosystem, Mars Pinkelman

COD Library Student Research and Award Symposium

The Spread of Batrachochytrium Dendrobatidis and its Effect on the Global Amphibian Ecosystem, discusses the deadly amphibian pathogen Batrachochytrium Dendrobatidis. My work aimed to answer the question, "How did the spread of batrachochytrium dendrobatidis, affect amphibian populations from its discovery in 1993 to modern day, and how could it affect biodiversity in the future?" It first covers a brief history of the disease and its discovery as well as the impacts it has on individual hosts. I then discuss the effects that the parasitic fungus has on biodiversity, concluding with ways for individuals and communities to mitigate the impacts that ...

The Effects Of Covid-19 On Heart Function, Samiha Syed

COD Library Student Research and Award Symposium

Student will investigate the heart function of heart patients who suffer from COVID-19 from a biological and physics-based lens and identify innovative treatment options to alleviate symptoms and blood flow. Students will seek faculty advising one-on-one with Dr. Purcell and additional, from other faculty members in the biology and physics departments at the College of DuPage. Student will also research ways to study heart function in the classroom and develop a model for further study.

Faculty Sponsor: Susan Purcell

A Tissue Specific Transcriptomic, Proteomic And Phospho-Proteomic Atlas Of The Translational Machinery Of Arabidopsis Thaliana, Abdullah Salim

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Gene expression encompasses the flow of genetic information from DNA to mRNA (transcription) and from mRNA to protein (translation) along with the regulatory mechanisms underlying these processes. Omics technologies offer a powerful toolset with which to study gene expression at each of these stages. A recently published dataset integrating transcriptomic, proteomic and phospho-proteomic measurements from 30 Arabidopsis thaliana tissues provides a unique resource to explore gene expression.¹ The translational machinery (the ribosome, and its initiation, elongation, and termination factors) are a core component in gene expression. Defects in translation can be lethal or lead to major developmental defects and ...

Expression And Purification Of N-Terminally Acetylated Microtubule Binding Protein Tau, Abigail Fleurima

Honors Projects Overview

The microtubule-associated protein Tau (MAPT) plays a critical role in many neurodegenerative diseases. Tau functions to stabilize microtubule structures that are essential for transport within the neuron, and transport disruption leads to loss of neuronal function. Tau binding is regulated by phosphorylation with the help of kinases that add phosphate groups which block microtubule binding sites, and phosphatases that remove phosphate groups and expose the microtubule-binding regions. Tau can be found in hyper-phosphorylated states, which causes Tau to self-assemble into aggregates and prevent microtubule binding. It's clear that post-translational modifications (PTMs) of Tau play a key role in the ...

Lexicographic Sensitivity Functions For Nonsmooth Models In Mathematical Biology, Matthew D. Ackley

Electronic Theses and Dissertations

Systems of ordinary differential equations (ODEs) may be used to model a wide variety of real-world phenomena in biology and engineering. Classical sensitivity theory is well-established and concerns itself with quantifying the responsiveness of such models to changes in parameter values. By performing a sensitivity analysis, a variety of insights can be gained into a model (and hence, the real-world system that it represents); in particular, the information gained can uncover a system's most important aspects, for use in design, control or optimization of the system. However, while the results of such analysis are desirable, the approach that classical ...

Evaluation Of Chili Pepper (Capsicum Annuum), Tiger Nuts (Cyperus Esculentus), And Turmeric (Curcuma Longa) As Sources Of Antioxidant Compounds For The Potential Of Antiaging-Like Activity, Chelsea Toyer

Theses

Aging is a natural process that living organisms go through as they become older. In humans and other animals, aging is accompanied by several morphological changes, including the formation of wrinkles. Wrinkles are also caused by various environmental factors such as excessive exposure to sun or pollution, and continuous sleep deprivation. These environmental factors cause oxidation of skin cells and the degradation of subcutaneous fatty acids, leading to the formation of wrinkles. Skin care and wrinkle treatment products are part of a multimillion-dollar industry. Plant-based products are an alternative method for treating wrinkles and premature aging. Several antioxidants naturally found ...

Big Five Personality Traits And Political Orientation: An Inquiry Into Political Beliefs, Ian E. Phillips

The Downtown Review

Personality research centered on the Big Five personality traits has heavily impacted our understanding in regards to what forces orient a person on a political spectrum. Examining how personality differences interact with political orientation, this research seeks to provide information on what makes someone either more or less likely to be liberal or conservative based on their temperament. In this paper, previous personality research is synthesized into one discussion, centered on what the effects of each trait are and how they impact political orientation, the heritability of personality, and what implications there are for such research in the realm of ...

Stage Of Estrous Cycle Influences Behavior After Social Stress, Alexander Jonah Rodriguez

Honors Thesis

Since women are almost twice as likely to develop major depression compared to men, research into this and other potentially debilitating mood disorders using behavioral studies should involve females despite hormonal, physiological, and potential behavior changes due to reproductive cyclicity. Until recently, most studies exclusively used males, thus limiting results of previous research to half of the population. Using adult female C57BL/6N mice as model organisms, we exposed them to novel forms of social stress across five days which encompassed the four-day estrous cycle to determine if the stages of the estrous cycle had an impact on behavior when ...

Terrestrial Soldier Crab (Coenobita Clypeatus, Fabricius 1787) And Cerion Spp. (Röding 1798) Shell Relationship On San Salvador Island, Bahamas, Harley Hunt

Biology Theses

The Caribbean terrestrial soldier crab, Coenobita clypeatus(Fabricius 1787), coexist and utilize the shells of numerous species of land and marine gastropods. Soldier crabs rely on gastropod shells for protection as the crabs have a soft abdomen, leaving them vulnerable for predation and desiccation, threatening their survival. This creates a strong pressure to obtain well-fitting shells that provide adequate protection against water loss. Cerion of Röding (1798) shells are one of the most commonly used shells among living colonies of C. clypeatuson San Salvador Island. This study is interested in the frequency of shell use by C. clypeatus crabs ...

A Year In Syntropy: Exploring Syntropic Agriculture, Ajah Eills

College Honors Program

Syntropic agriculture is a form of sustainable agriculture that originated in Brazil around 25 years ago. Although it has since spread throughout Brazil and Australia, there has yet to be a comprehensive study of the driving scientific principles behind syntropy. For my thesis, I conducted literature research and interviews with farmers, with the goal of describing the ecological principles on which syntropy is based, including its primary goal to improve soil health. Much of my thesis contrasted syntropic agriculture with conventional agriculture as practiced in the United States today, but I also explored the differences between syntropic agriculture and other ...

Assessing The Presence And Concentration Of Microplastics In The Gizzards Of Virginia Waterfowl, Thomas Bustamante

Student Research Submissions

Microplastics are defined as plastics smaller than 5mm which originate from sources such as manufactured pellets, personal care products, and the breakdown of larger plastic items. They have become a ubiquitous water pollutant, and while a substantial amount of research on their impacts on marine ecosystems has been conducted, the presence of microplastics in freshwater systems and organisms remains less understood. In this study, we assessed the presence and concentrations of microplastic particles in the gizzards of the Canada Goose (Branta canadensis), Longtailed Duck (Clangula hyemalis), Ringneck Duck (Aythya collaris), Mallard (Anas platyrhynchos), and Goldeneye Duck (Bucephala clangula) hunted in ...

Transmission Of Escherichia Coli Through Toilet Seats, Kenna Atienza, Diana Ludizaca, Carisa Davis

Kean Quest

Microbes are found on all kinds of surfaces, but not all are pathogenic. Bathrooms are perceived by the public as an important area in the transmission of pathogenic microbes, and the purpose of this project was to investigate the transmission of bacteria from toilet seats to skin. Escherichia coli strain K-12 was applied to the toilet seat and different methods were used to see which was the best way to prevent the transmission of bacteria to the skin. E. coli strain K-12 was identified in samples using the differential media, Eosin Methylene Blue agar. The experiment found that cleaning the ...

Late Pleistocene Rodents From Clark Quarry, A Vertebrate Fossil Locality In Southastern Georgia, Parker Rhinehart

Biology Theses

Clark Quarry is a Late Pleistocene fossil locality near Brunswick, GA, United States with a diverse vertebrate fauna. Morphological descriptions of Mammuthus columbi (Columbian Mammoth), snakes, amphibians, and birds from Clark Quarry have previously been completed along with a stable isotope analysis of M. columbi and Bison latifrons (Long-horned Bison) enamel. Herein, the rodent fauna is described using primarily teeth recovered by screen-washing quarry sediment. Nine rodent taxa have been identified, two of which are the extinct species Synaptomys australis (Florida Bog Lemming) and Neochoerus aesopi (Late Pleistocene Capybara). Of the remaining seven taxa, Erethizon dorsatum (North American Porcupine) and ...

Function Of Spiny Dorsal Fin Erector Muscles In The Bluegill, Lepomis Macrochirus, Zakiyat Djabakatie

Honors Projects Overview

Local motor control directly contributes to stability, which can be compromised by injury or multiple neuromuscular disorders. In addition, lack of sensory perception as experienced by decreased limb sensation can further deteriorate one’s quality of life. The goal of this study is to use bluegill (Lepomis macrochirus) fins as model systems to study and gain insights on local motor control and sensory perception to improve stability and locomotion in humans, especially in a rehabilitative state. We hypothesize that 1) when exposed to turbulence (T), bluegill will use the spiny dorsal fin to recover stability and muscle intensity and duration ...

Involvement Of The Ino80 Chromatin Remodeling Complex In Cell Division And Genomic Stability, Ethan Chen

Biomedical Engineering Undergraduate Honors Theses

Cell division is a vital biological process for growth and development in both single and multi-cellular organisms—whereby the cell must duplicate its organelles and genome in entirety and appropriately distribute the copied contents to the daughter cells. Cells undergo a cycle of two distinct phases: interphase and mitosis. During interphase, the cell replicates its genomic DNA (in the form of chromosomes) located within the nucleus. DNA replication is carried out in a euchromatin state, where the chromosome structure is loose and easily accessible by DNA polymerase and other replication enzymes. Upon the completion of replication, chromatin is condensed into ...

How Acetylation Regulates Metabolic Enzyme Function During Environmental Shifts, Jared Canonigo

Biological Sciences Undergraduate Honors Theses

Organisms such as Saccharomyces cerevisiae can regulate the mechanisms of proteins through post-translational modification. These modifications play a vital role in functional proteomic activity because they can regulate protein activity, localization, and interaction with other cellular molecules. Such modifications include phosphorylation, methylation, and acetylation. The metabolic mechanisms of yeast became of keen interest to our lab because our lab noticed many stress defense proteins were being acetylated during stress heat shock. Notably, Adh1p and Adh2p showed both an increase and a decrease in acetylation at two lysine residues (K315 and K314) overtime during heat shock respectively, though the exact function ...

Temperature-Dependent Macrophage Activity In Rainbow Trout, Danica Mcgrevey

Biological Sciences Undergraduate Honors Theses

Fever is an essential component of the immune response. Fever enhances immune responses as well as creates an environment in which the body has advantages over pathogens. Macrophages are often the first cells that come in contact with pathogens, as they reside in tissues. They are important for their engulfment of pathogens that results in the digestion of the pathogen, but they also produce nitric oxide and cytokines that contribute to immune response in a variety of ways, including initiating adaptive immunity and directing the production and activity of other immune cells. We were curious about macrophages from other temperature ...