Biology Commons^™

Mesenchymal Stem Cells In The Treatment Of Osteogenesis Imperfecta, Erica Lang, Julie A. Semon

Biological Sciences Faculty Research & Creative Works

Osteogenesis Imperfecta (OI) is a Disease Caused by Mutations in Different Genes Resulting in Mild, Severe, or Lethal Forms. with No Cure, Researchers Have Investigated the Use of Cell Therapy to Correct the Underlying Molecular Defects of OI. Mesenchymal Stem Cells (MSCs) Are of Particular Interest Because of their Differentiation Capacity, Immunomodulatory Effects, and their Ability to Migrate to Sites of Damage. MSCs Can Be Isolated from Different Sources, Expanded in Culture, and Have Been Shown to Be Safe in Numerous Clinical Applications. This Review Summarizes the Preclinical and Clinical Studies of MSCs in the Treatment of OI. Altogether, the …

Lead Exposure Of Mainland Australia's Top Avian Predator, Jordan O. Hampton, Michael T. Lohr, Aaron J. Specht, Damien Nzabanita, Jasmin Hufschmid, Lee Berger, Kate Mcginnis, Jane Melville, Emma Bennett, James M. Pay

Research outputs 2022 to 2026

Lead (Pb) toxicity, through ingestion of lead ammunition in carcasses, is a threat to scavenging birds worldwide, but has received little attention in Australia. We analyzed lead exposure in the wedge-tailed eagle (Aquila audax), the largest raptor species found in mainland Australia and a facultative scavenger. Eagle carcasses were collected opportunistically throughout south-eastern mainland Australia between 1996 and 2022. Lead concentrations were measured in bone samples from 62 animals via portable X-ray fluorescence (XRF). Lead was detected (concentration > 1 ppm) in 84% (n = 52) of the bone samples. The mean lead concentration of birds in which lead was detected …

The Importance Of Soil Carbon In Large-Scale Shrub Removal Practices In The Chihuahuan Desert, Kathleen Schaeffer

Open Access Theses & Dissertations

Shrub encroachment is a worldwide phenomenon that affects multiple biomes at different ecological and anthropogenic levels. In dryland ecosystems, shrub encroachment is a noted concern amongst land managers, as it can lead to a loss of soil resources and biodiversity. To mitigate the negative effects of shrub encroachment in Drylands, land managers can implement large-scale shrub removal practices. These land restoration practices can shift landscapes to novel ecosystems, where resulting plant communities can vary following treatments. Grass recovery or increasing herbaceous cover is often a primary goal of these treatments, however predicting which sites may show higher grass responses to …

Antimicrobial Peptides In Transgenic Silkworm Silk, David Jaden Turner

All Graduate Theses and Dissertations

Overview: People in India and China have produced silk textiles for thousands of years. Silk is a biodegradable, biocompatible compound used in the production of clothing, bedding, furniture, industrial materials, and medical applications. Over the last 30 years, research has increasingly investigated silk’s antimicrobial effects and how to augment its natural abilities. Antimicrobial peptides, or AMPs, are also an area of increasing interest as the rise of antibiotic resistance reduces the efficacy of current treatments. This project plans to systematically synthesize a fusion protein that incorporates the beneficial properties of each constituent into commercial silkworms.

Innovation: This project seeks to …

Physiological Consequences Of Adrenal Enlargement And Implications For Toxin Resistance In North American Snakes, Megen E. Kepas

All Graduate Theses and Dissertations

The long observed relationship between enlarged adrenal glands and toad-eating in snakes has remained a mystery in physiological ecology and herpetology. It has been predicted that large adrenal glands may be capable of producing higher amounts of key hormones, and that higher plasma hormone levels may lend some behavioral or physiological benefits after a toxic toad has been eaten. I address questions surrounding adrenal enlargement and its potential benefits to toad eaters in four research chapters. In my second chapter, I quantify adrenal hormone output at different embryonic stages in the toad-generalist snake Thamnophis elegans and examine the ability of …

Characterization Of Legionella Pneumophila Effector Proteins, Lneb And Mava, Kayode Adeyemi

Masters Theses & Specialist Projects

The crucial virulence factor of accidental human pathogen Legionella pneumophila during the course of Legionnaire disease is the over 300 effector proteins secreted from its Dot/Icm secretion system. Eukaryotic host cells usually elicit an arsenal of immune responses against invading L. pneumophila. Nonetheless, the bacteria unexpectedly subvert these defense mechanisms to survive and proliferate unhindered in the host. Although some effector proteins have been proposed to play a significant role in this host-pathogen interaction, many still need to be characterized. The LneB and MavA proteins are examples of those effectors that need characterization. Thus, this study aimed to investigate the …

Mammalian & Avian Community Response To African Elephant (Loxodonta Africana) Habitat Modification In Southeastern Kenya, Dakota Vaccaro

Masters Theses & Specialist Projects

African elephants (Loxodonta africana) play a significant role in the modification of their habitat, foremost by decreasing woody vegetation cover and density and in some parts of Africa high elephant densities damage small sanctuaries and preserves. For wildlife that depend on forested patches, this increased modification could lead to displacement and/or decreases in abundance. If suitable habitat is not available elsewhere, elephants and other wildlife may move closer to humans in search of resources, which can result in increased human-wildlife conflict. Evaluating this impact was one of the goals of the Elephants and Sustainable Agriculture in Kenya (ESAK) …

Science Communication Training Imparts Confidence And Influences Public Engagement Activity, Christina M. Swords, Jerlym S. Porter, Amy J. Hawkins, Edwin Li, Melissa Rowland-Goldsmith, Matthew D. Koci, John F. Tansey, Nicole C. Woitowich

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The impacts of science are felt across all socio-ecological levels, ranging from the individual to societal. In order to adapt or respond to scientific discoveries, novel technologies, or biomedical or environmental challenges, a fundamental understanding of science is necessary. However, antiscientific rhetoric, mistrust in science, and the dissemination of misinformation hinder the promotion of science as a necessary and beneficial component of our world. Scientists can promote scientific literacy by establishing dialogues with nonexperts, but they may find a lack of formal training as a barrier to public engagement. To address this, the American Society for Biochemistry and Molecular Biology …

Skeletal Anatomy Of The Pectoral Fin In Mudskipper Species From Terrestrial And Aquatic Habitats, Haodong Zhou, Cassandra M. Donatelli, Odette Laneuville, Emily M. Standen

Engineering Faculty Articles and Research

Mudskippers are a group of amphibious fishes in the family Oxudercidae, whose species inhabit a range of habitats from mostly aquatic to mostly terrestrial. Most of our understanding about habitat preference comes from natural history observations, particularly where they are collected (i.e., low intertidal vs. high intertidal regions). Mudskippers have undergone several morphological changes to accommodate a terrestrial life, including major changes to the pectoral and pelvic girdles. These changes result in a novel crutching gait, which mudskippers use to move over land. Though the appendicular morphology and crutching gait of mudskippers have been described in some species, few …

Effects Of Heterozygous Deletion Of Autism-Related Gene Cullin-3 In Mice, Qiang-Qiang Xia, Jeffrey Singer, Angela K. Walker, Chenghu Song, Multiple Additional Authors

Biology Faculty Publications and Presentations

Autism Spectrum Disorder (ASD) is a developmental disorder in which children display repetitive behavior, restricted range of interests, and atypical social interaction and communication. CUL3, coding for a Cullin family scaffold protein mediating assembly of ubiquitin ligase complexes through BTB domain substrate-recruiting adaptors, has been identified as a high-risk gene for autism. Although complete knockout of Cul3 results in embryonic lethality, Cul3 heterozygous mice have reduced CUL3 protein, demonstrate comparable body weight, and display minimal behavioral differences including decreased spatial object recognition memory. In measures of reciprocal social interaction, Cul3 heterozygous mice behaved similarly to their wild-type littermates. In …

Evolutionary Codependency: Insights Into The Mitonuclear Interaction Landscape From Experimental And Wild Caenorhabditis Nematodes, Suzanne Estes, Ulfar Bergthorsson, Vaishali Katju, Zachary P. Dietz

Biology Faculty Publications and Presentations

Aided by new technologies, the upsurgence of research into mitochondrial genome biology during the past 15 years suggests that we have misunderstood, and perhaps dramatically underestimated, the ongoing biological and evolutionary significance of our long-time symbiotic partner. While we have begun to scratch the surface of several topics, many questions regarding the nature of mutation and selection in the mitochondrial genome, and the nature of its relationship to the nuclear genome, remain unanswered. Although best known for their contributions to studies of developmental and aging biology, Caenorhabditis nematodes are increasingly recognized as excellent model systems to advance understanding in these …

Development Of A 16s Reference Library For Edna Metabarcoding The Freshwater Fishes Of Western Ecuador., Hannah M. Willis, Olivia G. Schweikart, Windsor E. Aguirre

DePaul Discoveries

This project examines the use of the 16S locus to amplify neotropical freshwater fishes native to Western Ecuador in a newly created 16S reference library for DNA barcoding and eDNA metabarcoding applications. Among the orders Characiformes, Siluriformes, Cichliformes, Gobiiformes, Cryprinodontiformes, Gymnotiformes, and Perciformes, a compendium of 105 specimens were sequenced, with 43 representing new 16S sequences previously unavailable on Genbank.

An Assessment Of The Impact Of Raman Based Glucose Feedback Control On Cho Cell Bioreactor Process Development, Luke Gibbons, Francis Maslanka, Nikky Le, Al Magill, Pankaj Singh, Joseph Mclaughlin, Fiona Madden, Ronan Hayes, Barry Mccarthy, Christopher Rode, Jim O'Mahony, Rosemary Rea, Caitlin O'Mahony-Hartnett

Department of Biological Sciences Publications

Process analytical technology (PAT) tools such as Raman Spectroscopy have become established tools for real time measurement of CHO cell bioreactor process variables and are aligned with the QbD approach to manufacturing. These tools can have a significant impact on process development if adopted early, creating an end-to-end PAT/QbD focused process. This study assessed the impact of Raman based feedback control on early and late phase development bioreactors by using a Raman based PLS model and PAT management system to control glucose in two CHO cell line bioreactor processes. The impact was then compared to bioreactor processes which used manual …

Westward Expansion Of The Evening Bat (Nycticeius Humeralis) Into Dawson And Lincoln Counties Of Central Nebraska, Jamila Bajelan, Keith Geluso

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The Evening Bat (Nycticeius humeralis) is a small, migratory species of temperate woodlands in the eastern United States. Along its western distributional edge in the Great Plains, this species resides in wooded riverine corridors. In recent decades, the distribution of Evening Bats has expanded westward throughout the Great Plains. Herein, we report on two county records that further document the distributional expansion of Evening Bats into central Nebraska. In 2019 and 2022, Evening Bats were collected in Lincoln and Dawson counties, respectively, in woodlands adjacent to the Platte River. Increases in wooded habitats along prairie waterways likely has …

The Manhattant: Identification, Natural History, And Nutritional Ecology Of A Recently Introduced Pest In New York City, Lasius Emarginatus (Hymenoptera: Formicidae), Samantha Kennett

Master of Science in Integrative Biology Theses

New York City is one of the oldest and largest cities in North America, and it is a center for global trade and shipping. As such, it has also been the site of introduction for some of North America’s most damaging invasive pests, from chestnut blight to the Asian long-horned beetle. Despite these cautionary examples, there has been no formal research on a newly introduced ant species that was tentatively identified as a European ant species, Lasius cf. emarginatus. Since its discovery in 2011, L. cf. emarginatus has become among the most common urban ant species in New York …

Cytotoxic Activity Of Salvia Officinalis Extract Mediated By The Inhibition Of Jak2 In Breast Cancer, Salma Alawadi Dawood

Theses and Dissertations

Background: Breast cancer is the most common type of invasive cancer in women in their forties and fifties. Recent evidence suggests that JAK2/STAT3 signaling is constitutively active in breast cancer. Previous studies suggest that plant extracts, including Salvia Officinalis, have strong cytotoxic effects on breast cancer cells. The differential expression of miRNAs is also strongly linked to cancer development.

Aim: In the current study, we hypothesize that S. Officinalis extract suppresses JAK2 expression and has strong anticancer potential in MCF7 breast cancer cell lines.

Methods: GC-MS analysis showed the presence of flavonoids in dried leaf of …

Pleurotus Spp. As Agents Of Mycoremediation: A Review, Colin Innes

University Honors Theses

Elemental and molecular contaminants of anthropogenic origin represent an increasing threat to virtually all terrestrial and aquatic environments as well as their surrounding atmosphere. The decontamination and restoration of these environments pose a significant and expensive challenge. Although chemical treatment or physical removal of contaminated substrates often is the most direct response to eliminate contaminants, due to the complexity and sensitivity of many natural systems, technologies involving biological remediation can provide viable alternatives. While plant, bacterial, and fungal organisms have the abilities to accumulate or to metabolize toxic compounds, fungal organisms are uniquely suited to do so. Their fast growth …

Alternatives To Antibiotics In Veterinary Medicine: Considerations For The Management Of Johne's Disease, Laura M. O'Connell, Aidan Coffey, Jim O'Mahony

Dept. of Biological Sciences Preprints

Antibiotic resistance has become a major health concern globally, with current predictions expecting deaths related to resistant infections to surpass those of cancer by 2050. Major efforts are being undertaken to develop derivative and novel alternatives to current antibiotic therapies in human medicine. What appears to be lacking however, are similar efforts into researching the application of those alternatives, such as (bacterio)phage therapy, in veterinary contexts. Agriculture is still undoubtedly the most prominent consumer of antibiotics, with up to 70 % of annual antibiotic usage attributed to this sector, despite policies to reduce their use in food animals. This not …

Bio 013: Writing In The Sciences - Human Origins, Esther Muehlbauer

Open Educational Resources

Bio. 013: Writing in the Sciences - Human Origins is an OER syllabus for a College Writing 2 course that develops student writing for different audiences, following the trajectory of how scientists work: Scientists Writing for themselves (a field journal); Scientists writing for other scientists (a review article); Scientists writing for society (an essay for Natural History Magazine).

A Remote Sensing Approach To Assess The Historical Invasion Of Phragmites Australis In A Brackish Coastal Marsh, Jason S. Hagani, John Y. Takekawa, Steven C. Chappell, Richelle L. Tanner, Adrienne R. Ernst, Karin M. Kettenring

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Introduction: Coastal estuarine wetlands provide important habitats for a variety of endemic flora and fauna but are particularly vulnerable to biological invasions. Regular monitoring of changes in these vulnerable wetlands has become increasingly important for effective management, especially considering threats from climate change effects and human disturbance. Historical analyzes of plant invasions may guide targeted management strategies to eradicate harmful species. Estimating the distribution of invasive species has never been more accessible with the improved availability of high-resolution data and innovations in remote sensing, estimating the distribution of invasive species has never been more accessible.

Methods: We assessed the spread …