Biology Commons^™

Mesenchymal Stem Cells In The Treatment Of Osteogenesis Imperfecta, Erica Lang, Julie A. Semon

Biological Sciences Faculty Research & Creative Works

Osteogenesis Imperfecta (OI) is a Disease Caused by Mutations in Different Genes Resulting in Mild, Severe, or Lethal Forms. with No Cure, Researchers Have Investigated the Use of Cell Therapy to Correct the Underlying Molecular Defects of OI. Mesenchymal Stem Cells (MSCs) Are of Particular Interest Because of their Differentiation Capacity, Immunomodulatory Effects, and their Ability to Migrate to Sites of Damage. MSCs Can Be Isolated from Different Sources, Expanded in Culture, and Have Been Shown to Be Safe in Numerous Clinical Applications. This Review Summarizes the Preclinical and Clinical Studies of MSCs in the Treatment of OI. Altogether, the …

The Importance Of Soil Carbon In Large-Scale Shrub Removal Practices In The Chihuahuan Desert, Kathleen Schaeffer

Open Access Theses & Dissertations

Shrub encroachment is a worldwide phenomenon that affects multiple biomes at different ecological and anthropogenic levels. In dryland ecosystems, shrub encroachment is a noted concern amongst land managers, as it can lead to a loss of soil resources and biodiversity. To mitigate the negative effects of shrub encroachment in Drylands, land managers can implement large-scale shrub removal practices. These land restoration practices can shift landscapes to novel ecosystems, where resulting plant communities can vary following treatments. Grass recovery or increasing herbaceous cover is often a primary goal of these treatments, however predicting which sites may show higher grass responses to …

Antimicrobial Peptides In Transgenic Silkworm Silk, David Jaden Turner

All Graduate Theses and Dissertations

Overview: People in India and China have produced silk textiles for thousands of years. Silk is a biodegradable, biocompatible compound used in the production of clothing, bedding, furniture, industrial materials, and medical applications. Over the last 30 years, research has increasingly investigated silk’s antimicrobial effects and how to augment its natural abilities. Antimicrobial peptides, or AMPs, are also an area of increasing interest as the rise of antibiotic resistance reduces the efficacy of current treatments. This project plans to systematically synthesize a fusion protein that incorporates the beneficial properties of each constituent into commercial silkworms.

Innovation: This project seeks to …

Physiological Consequences Of Adrenal Enlargement And Implications For Toxin Resistance In North American Snakes, Megen E. Kepas

All Graduate Theses and Dissertations

The long observed relationship between enlarged adrenal glands and toad-eating in snakes has remained a mystery in physiological ecology and herpetology. It has been predicted that large adrenal glands may be capable of producing higher amounts of key hormones, and that higher plasma hormone levels may lend some behavioral or physiological benefits after a toxic toad has been eaten. I address questions surrounding adrenal enlargement and its potential benefits to toad eaters in four research chapters. In my second chapter, I quantify adrenal hormone output at different embryonic stages in the toad-generalist snake Thamnophis elegans and examine the ability of …

Development Of A 16s Reference Library For Edna Metabarcoding The Freshwater Fishes Of Western Ecuador., Hannah M. Willis, Olivia G. Schweikart, Windsor E. Aguirre

DePaul Discoveries

This project examines the use of the 16S locus to amplify neotropical freshwater fishes native to Western Ecuador in a newly created 16S reference library for DNA barcoding and eDNA metabarcoding applications. Among the orders Characiformes, Siluriformes, Cichliformes, Gobiiformes, Cryprinodontiformes, Gymnotiformes, and Perciformes, a compendium of 105 specimens were sequenced, with 43 representing new 16S sequences previously unavailable on Genbank.

An Assessment Of The Impact Of Raman Based Glucose Feedback Control On Cho Cell Bioreactor Process Development, Luke Gibbons, Francis Maslanka, Nikky Le, Al Magill, Pankaj Singh, Joseph Mclaughlin, Fiona Madden, Ronan Hayes, Barry Mccarthy, Christopher Rode, Jim O'Mahony, Rosemary Rea, Caitlin O'Mahony-Hartnett

Department of Biological Sciences Publications

Process analytical technology (PAT) tools such as Raman Spectroscopy have become established tools for real time measurement of CHO cell bioreactor process variables and are aligned with the QbD approach to manufacturing. These tools can have a significant impact on process development if adopted early, creating an end-to-end PAT/QbD focused process. This study assessed the impact of Raman based feedback control on early and late phase development bioreactors by using a Raman based PLS model and PAT management system to control glucose in two CHO cell line bioreactor processes. The impact was then compared to bioreactor processes which used manual …

Westward Expansion Of The Evening Bat (Nycticeius Humeralis) Into Dawson And Lincoln Counties Of Central Nebraska, Jamila Bajelan, Keith Geluso

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The Evening Bat (Nycticeius humeralis) is a small, migratory species of temperate woodlands in the eastern United States. Along its western distributional edge in the Great Plains, this species resides in wooded riverine corridors. In recent decades, the distribution of Evening Bats has expanded westward throughout the Great Plains. Herein, we report on two county records that further document the distributional expansion of Evening Bats into central Nebraska. In 2019 and 2022, Evening Bats were collected in Lincoln and Dawson counties, respectively, in woodlands adjacent to the Platte River. Increases in wooded habitats along prairie waterways likely has …

Cytotoxic Activity Of Salvia Officinalis Extract Mediated By The Inhibition Of Jak2 In Breast Cancer, Salma Alawadi Dawood

Theses and Dissertations

Background: Breast cancer is the most common type of invasive cancer in women in their forties and fifties. Recent evidence suggests that JAK2/STAT3 signaling is constitutively active in breast cancer. Previous studies suggest that plant extracts, including Salvia Officinalis, have strong cytotoxic effects on breast cancer cells. The differential expression of miRNAs is also strongly linked to cancer development.

Aim: In the current study, we hypothesize that S. Officinalis extract suppresses JAK2 expression and has strong anticancer potential in MCF7 breast cancer cell lines.

Methods: GC-MS analysis showed the presence of flavonoids in dried leaf of …

Pleurotus Spp. As Agents Of Mycoremediation: A Review, Colin Innes

University Honors Theses

Elemental and molecular contaminants of anthropogenic origin represent an increasing threat to virtually all terrestrial and aquatic environments as well as their surrounding atmosphere. The decontamination and restoration of these environments pose a significant and expensive challenge. Although chemical treatment or physical removal of contaminated substrates often is the most direct response to eliminate contaminants, due to the complexity and sensitivity of many natural systems, technologies involving biological remediation can provide viable alternatives. While plant, bacterial, and fungal organisms have the abilities to accumulate or to metabolize toxic compounds, fungal organisms are uniquely suited to do so. Their fast growth …

Alternatives To Antibiotics In Veterinary Medicine: Considerations For The Management Of Johne's Disease, Laura M. O'Connell, Aidan Coffey, Jim O'Mahony

Dept. of Biological Sciences Preprints

Antibiotic resistance has become a major health concern globally, with current predictions expecting deaths related to resistant infections to surpass those of cancer by 2050. Major efforts are being undertaken to develop derivative and novel alternatives to current antibiotic therapies in human medicine. What appears to be lacking however, are similar efforts into researching the application of those alternatives, such as (bacterio)phage therapy, in veterinary contexts. Agriculture is still undoubtedly the most prominent consumer of antibiotics, with up to 70 % of annual antibiotic usage attributed to this sector, despite policies to reduce their use in food animals. This not …

Bio 013: Writing In The Sciences - Human Origins, Esther Muehlbauer

Open Educational Resources

Bio. 013: Writing in the Sciences - Human Origins is an OER syllabus for a College Writing 2 course that develops student writing for different audiences, following the trajectory of how scientists work: Scientists Writing for themselves (a field journal); Scientists writing for other scientists (a review article); Scientists writing for society (an essay for Natural History Magazine).

Vertebrate Animal Behaviors And Abundances On Estuarine Shorelines Stabilized With Biodegradable Materials Utilizing Wildlife Cameras, Katherine Guise

The Pegasus Review: UCF Undergraduate Research Journal

Living shoreline stabilization is a restoration technique that utilizes natural materials as breakwaters, plus vegetation landward of the breakwaters, to protect coastlines. This research does not provide information about how new, biodegradable restoration materials affect vertebrates that utilize these shorelines. For this project, I monitored 18 restoration sites along Canaveral National Seashore with wildlife trail cameras: 3 made with cement-infused jute breakwaters, 3 with metal gabion oyster shell breakwaters, and 4 with previously used breakwaters manufactured from plastic mesh oyster shell bags. This project used 4 sites as positive controls (intact vegetation) and 4 as negative controls (highly eroded, no …

Utilizing Few-Shot Meta Learning Algorithms For Medical Image Segmentation, Nick Littlefield

Thinking Matters Symposium

Deep learning models can be difficult to train because they require large amounts of data, which we usually do not have or are too expensive to get or annotate. To overcome this problem, we can use few-shot meta-learning, which allows us to train deep learning models with little data. Using a few examples, meta-learning, or learning-to-learn, aims to use the experience learned during training to generalize to unknown tasks. Medical imaging is an industry where it is particularly useful, as there is limited publicly available data due to patient privacy concerns and annotating costs.

This project examines how meta-learning performs …

Patrolling The Border: Billfish Exploit The Hypoxic Boundary Created By The World's Largest Oxygen Minimum Zone, Ryan Logan, Jeremy J. Vaudo, Brad Wetherbee, Mahmood Shivji

Biology Faculty Articles

Pelagic predators must contend with low prey densities that are irregularly distributed and dynamic in space and time. Based on satellite imagery and telemetry data, many pelagic predators will concentrate horizontal movements on ephemeral surface fronts—gradients between water masses—because of enhanced local productivity and increased forage fish densities.
Vertical fronts (e.g. thermoclines, oxyclines) can be spatially and temporally persistent, and aggregate lower trophic level and diel vertically migrating organisms due to sharp changes in temperature, water density or available oxygen. Thus, vertical fronts represent a stable and potentially energy rich habitat feature for diving pelagic predators but remain little explored …

Reintroducing Hemp (Rongony) In The Material Palette Of Madagascar: A Study On The Potential Of Hemp Clay Components And Its Impact On Social And Ecological Communities., Henintsoa Thierry Andrianambinina

Masters Theses

When mentioning the word hemp, especially in the local language of Madagascar, the literal translation does not set it apart from marijuana, as they are both called “rongony” - creating the stigma around hemp as the negative stereotype of marijuana. However, the material has been used by the ancestors of Madagascar, as well as across cultures, in its fibrous form to produce fabrication like textile goods and packaging. During colonization, the prohibition of hemp intensified, and since then, any activity related to either of these plants is prohibited and will end in severe punitive measures. This thesis explores the strengths …

Deconstructed Plastic Substrate Preferences Of Microbial Populations From The Natural Environment, Lindsay I. Putman, Laura G. Schaerer, Ruochen Wu, Daniel G. Kulas, Ali Zolghadr, Rebecca Ong, David Shonnard, Stephen Techtmann

Michigan Tech Publications

Over half of the world's plastic waste is landfilled, where it is estimated to take hundreds of years to degrade. Given the continued use and disposal of plastic products, it is vital that we develop fast and effective ways to utilize plastic waste. Here, we explore the potential of tandem chemical and biological processing to process various plastics quickly and effectively. Four samples of compost or sediment were used to set up enrichment cultures grown on mixtures of compounds, including disodium terephthalate and terephthalic acid (monomers of polyethylene terephthalate), compounds derived from the chemical deconstruction of polycarbonate, and pyrolysis oil …

Community-Derived Core Concepts For Neuroscience Higher Education, Audrey Chen, Kimberley A. Phillips, Jennifer E. Schaefer, Patrick M. Sonner

Biology Faculty Publications

Core concepts provide a framework for organizing facts and understanding in neuroscience higher education curricula. Core concepts are overarching principles that identify patterns in neuroscience processes and phenomena and can be used as a foundational scaffold for neuroscience knowledge. The need for community-derived core concepts is pressing, because both the pace of research and number of neuroscience programs are rapidly expanding. While general biology and many subdisciplines within biology have identified core concepts, neuroscience has yet to establish a community-derived set of core concepts for neuroscience higher education. We used an empirical approach involving more than 100 neuroscience educators to …

Metals And Metal Complexes In Diseases With A Focus On Covid-19: Facts And Opinions, Agnieszka Ścibior, Manuel Aureliano, Alvin A. Holder, Juan Llopis

Chemistry & Biochemistry Faculty Publications

In the present Special Issue on “Metals and Metal Complexes in Diseases with a Focus on COVID-19: Facts and Opinions”, an attempt has been made to include reports updating our knowledge of elements considered to be potential candidates for therapeutic applications and certain metal-containing species, which are extensively being examined towards their potential biomedical use due to their specific physicochemical properties. The Special Issue compiles data on the role of metals in COVID-19 and focuses on other illnesses and biological processes that affect metal metabolism. It consists of eight manuscripts, including five review articles and three original research papers (Figure …

Potential Impacts Of Environmental Bacteria On The Microbiota Of Loggerhead (Caretta Caretta) And Green (Chelonia Mydas) Sea Turtle Eggs And Their Hatching Success, Colleen Mcmaken, Derek Burkholder, Rosanna J. Milligan Dr., Jose V. Lopez

Biology Faculty Articles

Sea turtle hatching success can be affected by many variables, including pathogenic microbes, but it is unclear which microbes are most impactful and how they are transmitted into the eggs. This study characterized and compared the bacterial communities from the (i) cloaca of nesting sea turtles (ii) sand within and surrounding the nests; and (iii) hatched and unhatched eggshells from loggerhead (Caretta caretta) and green (Chelonia mydas) turtles. High throughput sequencing of bacterial 16S ribosomal RNA gene V4 region amplicons was performed on samples collected from 27 total nests in Fort Lauderdale and Hillsboro beaches in …

Novel Viruses Of The Family Partitiviridae Discovered In Saccharomyces Cerevisiae, Angela M. Crabtree, Ignacio De La Higuera, Kenneth Stedman, Multiple Additional Authors

Biology Faculty Publications and Presentations

It has been 49 years since the last discovery of a new virus family in the model yeast Saccharomyces cerevisiae. A large-scale screen to determine the diversity of double-stranded RNA (dsRNA) viruses in S. cerevisiae has identified multiple novel viruses from the family Partitiviridae that have been previously shown to infect plants, fungi, protozoans, and insects. Most S. cerevisiae partitiviruses (ScPVs) are associated with strains of yeasts isolated from coffee and cacao beans. The presence of partitiviruses was confirmed by sequencing the viral dsRNAs and purifying and visualizing isometric, non-enveloped viral particles. ScPVs have a typical bipartite …