Neuroscience and Neurobiology Commons^™

Optimization Of Tyrosine Hydroxylase Antibody For Immunohistochemistry Fluorescence Detection In Zebrafish (Danio Rerio), Madison Thurber

Theses/Capstones/Creative Projects

Dopamine is an important neurotransmitter produced through the catecholamine synthesis pathway that affects brain activity. Unregulated dopamine levels can lead to various diseases such as Parkinson’s Disease or attention deficit hyperactivity disorder (ADHD). Optimization of an immunohistochemistry protocol will allow for the quantification of tyrosine hydroxylase antibody, which indirectly allows for dopamine quantification in dopaminergic regions within the brain. However, the antibody concentration to give the optimal signal-to-noise ratio in IHC varies across studies. Through this experiment, I determined the concentration of tyrosine hydroxylase (TyrH) antibody for immunohistochemistry that gave the best signal-to-background noise ratio within several known dopaminergic regions …

Molecular Mechanisms Of Opioid Use Disorder In Human Brain Models, Emily Mendez

Dissertations & Theses (Open Access)

Opioid use disorder (OUD) is a national and global public health crisis with no end in sight. While studies from animal models hint at widespread epigenetic and transcriptomic alterations of opioid drugs, the molecular consequences of long-term exposure to opioid drugs in human brain is still unclear, and human-centered translational models are necessary to discern the human cell type-specific effects of OUD.

Using postmortem brain Brodmann area 9 (BA9) from the UTHealth Brain Collection for Research on Psychiatric Disorders, I identified angiogenic gene networks perturbed in the RNA and protein of OUD subjects, as well as downregulation of many neuron-correlated …

P300 Event-Related Potential Responses To Self-Relevant Stimuli, Jordan Razzak

Electronic Theses and Dissertations

Previous literature has suggested an apparent P300 sensitivity to self-relevant stimuli. To further explore this relationship, we asked participants to submit 10 photos, each of a particular category (e.g. footwear, plants), to be used as either targets or distractors in a given condition of an oddball task. Furthermore, we attempted to see whether the effect of self-relevance on the P300 could be induced in a participant by allowing them to study a set of unique photos which would then be used as targets. Our analysis suggested that P300 amplitude elicited in response to self-relevant stimuli used as targets was statistically …

The Effect Of The Glucocorticoid Receptor Modulator, Safit, On Acute Stress Reactivity In A Rat Model Of Abusive Head Trauma, Brielle Coe

Tower Health Research Day

No abstract provided.

In Silico Identification Of Small Molecule Agonist Binding Sites On Kcc2, Kenyon Mitchell, Alfred Amendolara, Ruth Hunter, Jaden Miner, Andrew Payne

Annual Research Symposium

Purpose: Potassium-Chloride Cotransporter 2 (KCC2) is a neuronal membrane protein specific to the central nervous system. It is responsible for removing Cl- ions from the intracellular space, maintaining a normal Cl- gradient essential for proper function at inhibitory synapses. Dysregulation causes an upward shift in the Cl- reversal potential resulting in a hyperexcitable state of the postsynaptic neuron. Existing literature indicates that KCC2 may be involved in the addiction pathway of a variety of drugs of abuse, including opioids and alcohol. This makes KCC2 an attractive potential drug target when treating substance use disorders. A novel direct KCC2 agonist, VU0500469, …

Technology To Support Aging In Place: The Perspective Of Caregivers, Sophie Meng

Capstone Research Posters

As the world population ages, more older adults prefer to age in a place which offers them better life qualities and autonomy but demands increased commitments of caregivers. The development of technology can be great aid in daily lives yet significant gaps persist in supporting older adults with impaired cognition aging in place. This study aimed to explore the perspective of caregivers on technology when assisting cognition impaired older adults at home. It sought to understand the needs of the caregivers in this context. The study also briefly addressed Artificial Intelligence (AI) in caregiving, considering its growing use. Five caregivers …

Associations Between Early Childhood Sleep, Memory Function, And Brain Development Across The Nap Transition, Sanna Lokhandwala

Doctoral Dissertations

Preschool-age children often distribute their sleep across a midday nap and overnight sleep. Skipping the nap is suggested to increase the duration and depth of deep sleep (i.e., slow wave activity; SWA). Moreover, missing the midday nap has been shown to impair learning processes. This may be because children’s brains at this point in development are immature, necessitating the intervening nap period to strengthen memories before they are forgotten. Nonetheless, at some point during the preschool years, many children begin transitioning naturally out of napping. It is unclear whether the memory benefits of overnight SWA after a skipped nap depend …

Zmp-Induced Activation Of Ampk Enhances Accumbal Dopamine Function, Isabella Roque, Jordan Yorgason, Rachel Campbell, Serin Baker, Jared Mcfarlane, Oliver Saunders, David Thomson

Library/Life Sciences Undergraduate Poster Competition 2024

Oral Presentation Honorable Mention

• Dopamine terminal function is associated with motivation

• Fasting activates AMPK, a cellular sensor that measures intracellular AMP relative to ATP

• AMPK activation is a target of interest for age related cognitive decline, prevention of muscle wasting, etc.

• ZMP is an endogenous nucleotide AMP mimetic that activates AMPK

• The present study examined the novel precursor ZMP compound called P39 using an ex vivo mouse model

Understanding Refractory Status Epilepticus With Novel Hd-Mea Technology, Isaac Stubbs, Ryley Parrish, Melissa Blotter, Max Holmes, Skyler Russell

Library/Life Sciences Undergraduate Poster Competition 2024

3rd Place Oral Presentation

Status Epilepticus (SE) is a severe medical condition marked by continuous seizures lasting over 5 minutes. When SE becomes resistant to anticonvulsant drugs, the condition is known as Refractory Status Epilepticus (RSE), which lacks effective treatments and has a mortality rate of 38%. RSE lacks effective treatments partially due to our limited understanding of the mechanisms that lead to patient antiepileptic drug resistance.

Ivermectin Activates Striatal Cholinergic Interneurons To Enhance Dopamine Release, Tyler Love, Jordan Yorgason, Hillary Wadsworth, Alicia Warnecke, Joshua Barlow, Emma Steimle, Joakim Ronström, Pacen Williams, Christopher Galbraith, Jared Baldridge, Michael Jakowec, Daryl Davies

Library/Life Sciences Undergraduate Poster Competition 2024

• The substantia nigra compacta releases dopamine into the dorsal striatum, a brain region associated with movement and motivation

• Cholinergic interneurons in the dorsal striatum can directly cause dopamine release

• IVM is a known positive allosteric modulator of P2X4 receptors as well as nicotinic

• acetylcholine receptors

• Ivermectin is known to affect many dopamine linked behaviors, but no studies have been conducted to determine if Ivermectin is able to modulate dopamine release

Effects Of Predation On Cognition In Brachyrhaphis Rhabdophora, Elizabeth F. Jenkins, Jerry B. Johnson

Library/Life Sciences Undergraduate Poster Competition 2024

RESEARCH QUESTION

Does “predation environment” affect the cognitive abilities of the tropical fish Brachyrhaphis rhabdophora?

Effects Of Methamphetamine On Microglia Through Reactive Oxygen Species, Chase Seiter, Jordan Yorgason, Nathan Sheets, James Blood, Lydia Hawley, Erin Taylor, Eliza White, Hillary Wadsworth, Jason Hansen

Library/Life Sciences Undergraduate Poster Competition 2024

▪ Dopamine release in the Nucleus Accumbens (NAc) underlies motivational behavior for methamphetamine (METH) reward.

▪ ATP is a chemoattractant to microglia and is METH on ATP release and clearance are unknown.

▪ Furthermore, METH is known to produce reactive dopamine terminal function, microglia morphology and METH interactions are unknown.

Adhd Medication Is More Potent And Effective During Ovulation, Laurenf@Byu.Edu Ford

Library/Life Sciences Undergraduate Poster Competition 2024

• Women escalate psychostimulant abuse more quickly than men. Women who are prescribed psychostimulants for ADHD report fluctuations in drug efficacy that correspond to their menstrual cycle.

• In rodents, females acquire psychostimulant addiction more readily and release more dopamine (DA) in the dorsal striatum, an effect mediated by estrogen.

• However, the impact of the estrous cycle in the nucleus accumbens (NAc), a core region for learning and addiction in the ventral striatum, is underexplored.

• Here, we examine cocaine, methylphenidate (Ritalin), and methamphetamine (Desoxyn) effects on DA transmission in male and naturally-cycling female mice.

Adapting The Auxin-Inducible Degron (Aid) System To The Zebrafish Model Organism, Dennis Li

Library/Life Sciences Undergraduate Poster Competition 2024

The Auxin Inducible Degron (AID) system is a conditional and reversible protein degradation system naturally occurring in plants. In the presence of the hormone Auxin, the AID tagged proteins are recruited into the plant’s E3 ubiquitin ligase machinery, ubiquitinated, and marked for degradation1. To adapt the AID system to the zebrafish model organism, our lab generated lines co-expressing Arabidopsis Thaliana plant TIR1 protein and GFP tagged with AID (GFP-AID) in superficially-located mechanosensory hair cells (HC) of the lateral line system and in spinal cord neurons found deeper in the body. Upon auxin application, GFP-AID expressed in the superficial HCs was …

The Binding And Mechanism Of A Positive Allosteric Modulator Of Kv3 Channels, Qiansheng Liang, Gamma Chi, Leonardo Cirqueira, Lianteng Zhi, Agostino Marasco, Nadia Pilati, Martin Gunthorpe, Giuseppe Alvaro, Charles Large, David Sauer, Werner Treptow, Manuel Covarrubias

Farber Institute for Neuroscience Faculty Papers

Small-molecule modulators of diverse voltage-gated K⁺ (Kv) channels may help treat a wide range of neurological disorders. However, developing effective modulators requires understanding of their mechanism of action. We apply an orthogonal approach to elucidate the mechanism of action of an imidazolidinedione derivative (AUT5), a highly selective positive allosteric modulator of Kv3.1 and Kv3.2 channels. AUT5 modulation involves positive cooperativity and preferential stabilization of the open state. The cryo-EM structure of the Kv3.1/AUT5 complex at a resolution of 2.5 Å reveals four equivalent AUT5 binding sites at the extracellular inter-subunit interface between the voltage-sensing and pore domains of the …

Exploring Neuroplasticity Changes In Neurotoxin-Induced Parkinson’S Disease: A Preliminary Analysis Using Transcranial Magnetic Stimulation, Tomas Gomez Jr., Daniel Salinas, Kelsey Potter-Baker, Nawaz Hack, Ramu Vadukapuram

Research Symposium

Background: Parkinson’s disease (PD) is a neurodegenerative condition that affects movement, cognition, gait, and significantly impacts one's quality of life. Studies have suggested that neurotoxin pre-exposure is related to PD pathology and progressive motor/non-motor deficits, though it remains unclear how neurotoxin exposure affects neuroplasticity. The present study aimed to examine neurotoxin–induced PD-associated neuroplasticity changes in relationship to mental acuity and PD motor functionalities.

Methods: 7 voluntary participants experiencing early-stage PD symptoms with self-reported neurotoxin pre-exposure were enrolled in the longitudinal, repeated-measures clinical study; 2 sex-matched, age-matched, and occupation-matched healthy subjects were recruited for controlled comparative analysis (n=9). UTRGV’s Institute …

Does Joint Angle Affect Composite, Inter-Individual, And Intra-Individual Patterns Of Responses For Men During Fatiguing Isometric Forearm Flexion Tasks Anchored To A High Perceptual Intensity?, Jocelyn E. Arnett, Robert W. Smith, Tyler J. Neltner, John Paul V. Anders, Dolores G. Ortega, Terry J. Housh, Richard J. Schmidt, Glen O. Johnson

Journal for Sports Neuroscience

Purpose: The purpose of this study was to utilize the Ratings of Perceived Exertion (RPE) Clamp Model to examine the effects of joint angle on the composite, inter-, and intra-individual patterns of responses for torque and neuromuscular parameters during fatiguing isometric tasks anchored to RPE of 8 (RPE = 8) at elbow joint angles of 75° (JA75) and 125° (JA125) for men. Methods: Ten men (Mean ± SD: age: 20.7 ± 1.2 yrs; height: 181.6 ± 6.0 cm; body mass: 83.7 ± 14.9 kg) performed 2,3 s forearm flexion maximal voluntary isometric contractions (MVIC) with the dominant arm before and …

A Potential Role Of Urinary P75ecd As A Biomarker For Amyotrophic Lateral Sclerosis In An American Cohort, Swati Dhasmana, Anupam Dhasmana, Sheema Khan, Acharan S. Narula, Murali Yallapu, Subhash Chauhan

Research Symposium

Background: Neurological disorders present a unique complexity compared to other diseases, involving multiple risk factors, causes, treatments, and outcomes. These disorders often exhibit various molecular and morphological changes indicative of disruptions in cellular plasticity and resilience. The pathogenesis of many neurological disorders remains unclear, necessitating ongoing investigations. Amyotrophic lateral sclerosis (ALS) exemplifies an idiopathic and fatal neurodegenerative disease marked by the degeneration of upper and lower motor neurons. The average life expectancy post-diagnosis is a mere 36 months, primarily attributed to respiratory muscle denervation.The persistent challenges in ALS clinical trials and the absence of effective therapeutic options have intensified interest …

Exploring The Developmental Effects Of Antidepressants In Caenorhabditis Elegans, Alexandra Van Stone

Annual Research Symposium

It is so important to explore the potential side effects of antidepressants especially if these side effects could lead to a future detriment to an individual’s financial and social well-being. A better understanding of these side effects in regards to human developmental issues is imperative considering issues related to mental health and depression affect a large percentage of the population. In this study, we tested if Caenorhabditis (C.) elegans could be a potential model organism to predict possible side effects associated with select antidepressants. Their developmental problems potentially encountered upon drug treatment may be encountered in humans.

Elucidating Neuroinflammation In Multiple Sclerosis By Network Analysis, Nora C. Welsh

Dartmouth College Ph.D Dissertations

Multiple sclerosis (MS) is a heterogeneous disease, differing on many variables, including disease course, sex, and overall activity. Key characteristics of the disease encompass demyelination, axonal damage, neuronal loss, glial cell activation, and the infiltration of peripheral immune cells. Molecular proxies of these functions are secreted proteins, including cytokines and immunoglobulins, which, in the central nervous system (CNS), can be secreted into the cerebrospinal fluid (CSF). A detailed analysis of these secreted proteins can offer insights into the evolving immunological and neurodegenerative features as the disease progresses. To understand the dynamic biological processes involved in MS, I used network analysis …