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Articles 1 - 30 of 3127
Full-Text Articles in Biotechnology
Phytochemical Analysis And Safety Assessment Of Watercress Nasturtium Officinale: Antioxidant Capacity, Heavy Metal Profiling, And Toxicity Using Brine Shrimp Model, Qurrota A’Yunin, Chamini Kasun Hemachandra, Muhaimin Rifa’I, Yoga Dwi Jatmiko, Maftuch Maftuch, Rizky Fadilla Agustin Rangkuti, Cucun Herlina, Zulfa Mahdiatur Rasyida, Moh Dliyauddin
Phytochemical Analysis And Safety Assessment Of Watercress Nasturtium Officinale: Antioxidant Capacity, Heavy Metal Profiling, And Toxicity Using Brine Shrimp Model, Qurrota A’Yunin, Chamini Kasun Hemachandra, Muhaimin Rifa’I, Yoga Dwi Jatmiko, Maftuch Maftuch, Rizky Fadilla Agustin Rangkuti, Cucun Herlina, Zulfa Mahdiatur Rasyida, Moh Dliyauddin
Karbala International Journal of Modern Science
Medicinal plants have attracted growing attention as sources of natural bioactive compounds for sustainable aquaculture and health. This study evaluated the phytochemical composition, antioxidant activity, heavy metal profile, and acute toxicity of watercress (Nasturtium officinale W.T. Aiton) methanol extract using maceration, qualitative and quantitative (TPC, TFC, antioxidant DPPH) phytochemical assay, XRF, and BSLA. Phytochemical screening of plant extracts showed the existence of flavonoids, alkaloids, phenols, tannins, triterpenoids, and saponins, but not steroidal. The TPC and TFC were 20.6 mg GAE/g and 10.8 mg QE/g, respectively. The extract displayed significant antioxidant activity (IC50 = 64.78 µg/mL in DPPH assay). …
Electroceuticals In Neuromodulation: Mechanisms, Clinical Applications, And Future Directions, Albashir Tahir, Nura Bello
Electroceuticals In Neuromodulation: Mechanisms, Clinical Applications, And Future Directions, Albashir Tahir, Nura Bello
AUIQ Complementary Biological System
Electroceuticals have emerged as an innovative therapeutic approach that employs targeted electrical stimulation to modulate neural circuits and restore physiological balance. Unlike conventional pharmacological treatments, which act through systemic biochemical pathways, electroceuticals enable precise spatial and temporal control of neural activity. The rapid proliferation of electroceutical devices across clinical and investigational settings, combined with accelerating advances in artificial intelligence, closed-loop engineering, and bioelectronic interface design, has generated an urgent need for rigorous, critically appraised synthesis to guide evidence-based clinical adoption and inform future research priorities. This review addresses the rapidly expanding field of bioelectronic medicine by providing a critical synthesis …
In Vivo Expression Profiling Of Listeria Monocytogenes Internalins (Inlf, Inlp, Inlj) And Their Corresponding Host Receptors (Vim, Afdn, Muc2) Using A Rabbit Ligated Intestinal Loop Model, Nawar Al-Janabi, Nadhim Mushtaq Hashim Al-Bderee, Zahraa M. Madania, Mohanad Jawad Kadhim
In Vivo Expression Profiling Of Listeria Monocytogenes Internalins (Inlf, Inlp, Inlj) And Their Corresponding Host Receptors (Vim, Afdn, Muc2) Using A Rabbit Ligated Intestinal Loop Model, Nawar Al-Janabi, Nadhim Mushtaq Hashim Al-Bderee, Zahraa M. Madania, Mohanad Jawad Kadhim
Karbala International Journal of Modern Science
Listeria monocytogenes is a Gram-positive pathogen that causes severe infections, particularly in immunocompromised individuals. Internalin genes are important virulence factors that facilitate the bacterium's invasion of host cells. This research aimed to quantify in vivo expression of selected internalin genes (inlF, inlP, and inlJ) and their corresponding host receptors (VIM, AFDN, and MUC2) during L. monocytogenes infection. To achieve this aim, a rabbit ligated intestinal loop model was constructed comprising two intestinal loops. The first loop was injected with 1 mL of L. monocytogenes (1 × 10⁷ CFU/mL), while the second loop was injected with 1 mL of PBS as …
Effectiveness Of Organic Amendments In Enriching Nitrogen In Compost Produced From Organic Fractions Of Market Waste Generated In Dar Es Salaam, Tanzania, Amani Bilula, Ally Mahadhy, Anthony Manoni Mshandete, Amelia Kivaisi
Effectiveness Of Organic Amendments In Enriching Nitrogen In Compost Produced From Organic Fractions Of Market Waste Generated In Dar Es Salaam, Tanzania, Amani Bilula, Ally Mahadhy, Anthony Manoni Mshandete, Amelia Kivaisi
Tanzania Journal of Science
Low nitrogen (N) content remains a key limitation of compost derived from urban organic waste. This study evaluated the effectiveness of selected plant-based organic amendments in enhancing nitrogen content and improving compost quality. Four plant- based organic amendments, Leucaena leucocephala (BN01), Commelina benghalensis (BN02), Morus alba (BN03), and Helianthus annuus (sunflower) cake (BN04), were incorporated into market waste compost at ratios of 1:0.06:0.04, 1:0.06:0.25, and 1:0.06:0.5 (organic waste: sawdust: amendment). Composting was conducted using a 4 × 3 factorial design with three replicates per treatment (n = 36). Temperature, moisture, and pH were monitored throughout the composting period. Total nitrogen, …
Advances In Gene Editing Strategies For Viral Disease Resistance In Pigs: Knock-Out, Knock-In, And Overexpression Approaches, Zhao Namula, Takeshige Otoi, Aya Nakai, Megumi Nagahara, Maki Hirata, Fuminori Tanihara
Advances In Gene Editing Strategies For Viral Disease Resistance In Pigs: Knock-Out, Knock-In, And Overexpression Approaches, Zhao Namula, Takeshige Otoi, Aya Nakai, Megumi Nagahara, Maki Hirata, Fuminori Tanihara
The Thai Journal of Veterinary Medicine
Highly contagious viral pathogens—such as porcine reproductive and respiratory syndrome, classical swine fever, and African swine fever—continue to pose serious threats to the swine industry and cause major global economic losses. Conventional disease control approaches, such as vaccination and biosecurity, are limited by viral heterogeneity and immune escape mechanisms. Gene editing technologies, most notably the CRISPR/Cas9 system, offer an innovative means of introducing innate viral disease resistance or resilience in pigs. The continuous improvement of these tools can provide a sustainable strategy to enhance animal welfare and global food security and reduce reliance on traditional control measures. This review provides …
First Photographic Record Of A Pale-Colored Wild Boar (Sus Scrofa) Consistent With Leucism In The Zagros Mountains, Iraqi Kurdistan, Bahez F. Ali, Hana A. Raza, Nabaz Horeni
First Photographic Record Of A Pale-Colored Wild Boar (Sus Scrofa) Consistent With Leucism In The Zagros Mountains, Iraqi Kurdistan, Bahez F. Ali, Hana A. Raza, Nabaz Horeni
AUIQ Complementary Biological System
Pigmentation anomalies in wild mammals are rare and poorly documented in wild boar (Sus scrofa) populations. Leucism, characterized by partial loss of melanin while retaining normal eye pigmentation, represents one of the least frequently reported coloration anomalies in the species. During a camera-trap survey in Bamo Mountain, Zagros Mountains of Iraqi Kurdistan, an unusually pale-colored wild boar consistent with leucism was recorded. The individual exhibited cream to light-blond pelage with retained darker pigmentation on the snout and distal limbs, while the eyes remained normally pigmented. Its morphology and behavior were otherwise consistent with those of a typical wild …
Uti, Delirium, And Dementia: A Conceptual Bioinformatics Framework For Clinical Attribution, Rob E. Carpenter, Andrew Krouse
Uti, Delirium, And Dementia: A Conceptual Bioinformatics Framework For Clinical Attribution, Rob E. Carpenter, Andrew Krouse
Human Resource Development Faculty Publications and Presentations
Urinary tract infection (UTI) is frequently invoked to explain delirium, confusion, and functional decline in persons with dementia. Yet urine positivity in this population often reflects asymptomatic bacteriuria, colonization, chronic pyuria, contamination, or sampling noise rather than clinically attributable infection. For biomedical informatics and computational diagnostics readers, this narrative review contributes an integrative conceptual bioinformatics framework for probabilistic clinical attribution of suspected UTI in dementia under uncertainty. We formalizes the attribution problem by distinguishing organism detection from a clinically plausible infection-related contribution to neurocognitive decline. The framework integrates clinical, laboratory, biological, and longitudinal data to clarify whether urinary findings are …
A Review On Nanoparticles: Classification And Roles In Plant Systems, Muhammad Junaid Zafar, Muhammad Shafiq, Muhammad Azeem Rana
A Review On Nanoparticles: Classification And Roles In Plant Systems, Muhammad Junaid Zafar, Muhammad Shafiq, Muhammad Azeem Rana
Journal of Bioresource Management
Nanotechnology has been a new paradigm of the twenty-first century with several good breakthroughs. These tiny particles, known for their outstanding qualities, are enjoying an increasing influence in many research fields concerning foodstuff, cosmetics, electronics, and pharmacology. Synthesis of nanoparticles includes utilizing chemical processes with reducing agents, alongside employing physical techniques such as top-down and bottom-up approaches for NP formation. The biological approach, known as green synthesis, utilizes plant extracts to ensure increased safety. Biological methods also involve bacteria and fungi, known as the major sources of nanoparticles. It is acknowledged as the most effective and environmentally conscious approach, attributed …
In Vitro Anticancer Evaluation Of Tamoxifen And Paclitaxel-Loaded Chitosan Nanoparticles Against Breast And Lung Cancer With In Silico Docking, Arshia Amaan, Farah Mehak, Amer Jamil, Fatma Hussain
In Vitro Anticancer Evaluation Of Tamoxifen And Paclitaxel-Loaded Chitosan Nanoparticles Against Breast And Lung Cancer With In Silico Docking, Arshia Amaan, Farah Mehak, Amer Jamil, Fatma Hussain
Journal of Bioresource Management
Breast and lung cancer are the primary cause of death worldwide, underlining the need for an improved therapeutic strategy. In the current study, tamoxifen (TAM) and paclitaxel (PTX) loaded chitosan nanoparticles (NPs) were produced for the evaluation of sustained drug delivery and enhanced anticancer potential of nanocarriers over conventional chemotherapy against breast and lung cancers, respectively. Nanoparticles were produced by the ionic gelation method and characterized by UV-visible spectroscopy, Dynamic Light Scattering and Fourier Transform Infrared Microscopy (FTIR), verifying the nanoscale morphology, stability and successful encapsulation. A drug release profile study (in vitro) (78 %, 60-75 %) and …
Endophytic Actinobacteria: Deciphering Therapeutic And Industrial Potential, And Prospects Of Genome Mining In Novel Products Discovery, Yusra Waheed, Rabia Shahdin, Imran Sajid
Endophytic Actinobacteria: Deciphering Therapeutic And Industrial Potential, And Prospects Of Genome Mining In Novel Products Discovery, Yusra Waheed, Rabia Shahdin, Imran Sajid
Journal of Bioresource Management
Endophytic actinobacteria are a diverse group of Gram-positive bacteria with high G+C content, which inhabit plants’ internal tissues, and represent a significant yet underexplored reservoir of bioactive secondary metabolites. In host plants, a remarkable repertoire of metabolites, including broad-spectrum antibiotics, anticancer, antiviral, antifungal, antioxidant, and immunomodulatory compounds, is synthesized by these microbial allies, carrying significant medicinal and agricultural potential. These endophytes, in contrast to their soil relatives, have developed unique biosynthetic gene clusters (BGCs) strongly influenced by plant-associated factors, leading to the production of structurally novel metabolites. In recent years, the advances in genome mining, metagenomics, and metabolomics have accelerated …
Biotechnological Applications Of Microalgae: Advances, Potential And Future Challenges: A Review, Samiha M. Gharib, Mohamed Z. Nassar
Biotechnological Applications Of Microalgae: Advances, Potential And Future Challenges: A Review, Samiha M. Gharib, Mohamed Z. Nassar
Blue Economy
This review highlights the diverse biotechnological applications of microalgae, emphasizing their significance in human health, nutrition, and sustainable development. Microalgae are among the most widespread photosynthetic microorganisms in aquatic environments, characterized by remarkable metabolic diversity and the ability to produce a wide range of valuable bioactive compounds. These compounds include proteins, lipids, essential fatty acids, pigments, vitamins, and antioxidants, which support their applications in food, feed, pharmaceuticals, and cosmetic industries. In addition to their nutritional and therapeutic potential, microalgae play a crucial role in environmental management through carbon dioxide sequestration, wastewater treatment, and bioremediation processes. Their capacity to convert solar …
Screening For The Suitable Signal Peptide For Secreted Nattokinase Expression In E. Coli Using An In Silico Approach: Precursor Vs. Mature Forms, Dewi Purwani Caya Ningsih, Kartika Sari Dewi, I Wayan Martadi Santika, Ade Andriani, Joko Pebrianto Trinugroho, Muhammad Yusuf
Screening For The Suitable Signal Peptide For Secreted Nattokinase Expression In E. Coli Using An In Silico Approach: Precursor Vs. Mature Forms, Dewi Purwani Caya Ningsih, Kartika Sari Dewi, I Wayan Martadi Santika, Ade Andriani, Joko Pebrianto Trinugroho, Muhammad Yusuf
Makara Journal of Science
Nattokinase (NK), an enzyme with thrombolytic activity, has been widely used in treating cardiovascular disease. The expression of NK in the E. coli cell host is favored due to its several benefits, though it often results in inclusion bodies. Appropriate signal peptides can be attached to the N-terminus of a protein to initiate a secretory expression system, thus preventing the nascent protein from aggregating in the cytoplasm. This study aimed to identify suitable signal peptides for the secretory expression of active NK in E. coli and to investigate the impact of pre-protein in NK secretion using an in silico approach. …
Contribution Of Animals To Biomedical Advancements In The Current Era: A Narrative Review, Leena Rajathy Port Louis, Mani Rajarathinam, Prithiviraj Nagarajan, Divya Sekar, Nisha Perumal
Contribution Of Animals To Biomedical Advancements In The Current Era: A Narrative Review, Leena Rajathy Port Louis, Mani Rajarathinam, Prithiviraj Nagarajan, Divya Sekar, Nisha Perumal
The Thai Journal of Pharmaceutical Sciences
Animal research has played a major role in the development of vaccinations and therapies for illnesses such as tetanus, polio, meningitis, hepatitis, and Severe Acute Respiratory Syndrome Coronavirus 2. For every prescription drug available today, animal testing and research have contributed immensely. This narrative review aims to explore the contribution of animals to biomedical advancements in the current era, which involved a search of peer-reviewed literature using electronic databases such as Scopus, Web of Science, and PubMed. In current biomedical research, animals help develop new treatments for long-term illnesses such as cancer, HIV, and Alzheimer’s. Animals also aid in the …
Meningioma Detection From Mri: A Comparative Study Of Deep Learning Models, A. H. Doha, Sandi Jennifer, Fatima Alabdaly
Meningioma Detection From Mri: A Comparative Study Of Deep Learning Models, A. H. Doha, Sandi Jennifer, Fatima Alabdaly
AUIQ Complementary Biological System
This study compares several deep learning models including DenseNet20, CNN, ResNet50, VGG19, EfficientNet-B3, MobileNetV2, NASNetMobile, Xception to automatically classify brain meningiomas from MRI scans. A public dataset was used to evaluate the proposed model. All models were trained, validated, and tested on this dataset. Because meningiomas are common and manual reads, the goal is to speed and standardize diagnosis. Our main contribution is pairing a high-accuracy classifier with deep learning models. Among all models, DenseNet201 performed best, reaching 0.991% accuracy and reliably separating tumorous from non-tumorous MRIs. Future work will expand the dataset and incorporate stronger segmentation to further boost …
Metal Oxide Nanoparticle‑Integrated Phema Hydrogels: Morphology, Chemical Properties, And Biological Interaction, Venkateswar Rao, Collin Cox, Brandon Clark, Arushi Rai, Erick S. Vasquez-Guardado, Madhuri Kango-Singh, Soubantika Palchoudhury
Metal Oxide Nanoparticle‑Integrated Phema Hydrogels: Morphology, Chemical Properties, And Biological Interaction, Venkateswar Rao, Collin Cox, Brandon Clark, Arushi Rai, Erick S. Vasquez-Guardado, Madhuri Kango-Singh, Soubantika Palchoudhury
Chemical and Materials Engineering Faculty Publications
Hydrogels offer attractive matrices for incorporating inorganic metal oxide nanoparticles (NPs) to generate multifunctional hybrid materials, but challenges remain in scalable synthesis and correlating their properties to bio-interaction. Therefore, we report the synthesis and characterization of poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels, and pHEMA hydrogels encapsulating iron oxide or zinc oxide NPs, using a modified free-radical polymerization. Dose-dependent survivorship studies on Drosophila melanogaster revealed that both NP-embedded hydrogels are tolerated over a wide dose range (e.g., 50–200 mg), although ZnO NP-gels exhibit lower survivorship at some concentrations due to their inherent composition and a wider size distribution of iron oxide NPs.
Discovery Of Bmi-Associated Gut Microbiome Signatures In The Awi-Gen 2 Cohort, Meriem Yagoubi
Discovery Of Bmi-Associated Gut Microbiome Signatures In The Awi-Gen 2 Cohort, Meriem Yagoubi
Theses and Dissertations
Obesity is increasing across sub-Saharan Africa amid rapid dietary, environmental, and urbanization transitions, yet the gut microbiome features associated with adiposity in African populations remain under-characterized. This study investigated whether species-level gut microbiome composition is associated with body mass index (BMI) in the AWI-Gen 2 cohort, and whether BMI-associated microbial signals remain detectable after accounting for the dominant geographic structure of the microbiome. Gut microbiome community structure and diversity were characterized across 1,665 participants, and differential abundance analysis was used to identify taxa associated with BMI after controlling for study site. Machine learning models were then trained to assess how …
Diabetes Mellitus Exacerbates Covid-19 Severity And Prolongs Recovery: A Clinical Study From Southern Iraq, Hussein Ali Hussein Al-Hchaimi, Mohammad Ali Hassan Jassim, Mustafa Abbass, Shahab Abdulla, Ralela Makline
Diabetes Mellitus Exacerbates Covid-19 Severity And Prolongs Recovery: A Clinical Study From Southern Iraq, Hussein Ali Hussein Al-Hchaimi, Mohammad Ali Hassan Jassim, Mustafa Abbass, Shahab Abdulla, Ralela Makline
AUIQ Complementary Biological System
Diabetes mellitus (DM) constitutes one of the most consequential comorbidities shaping the clinical trajectory of Coronavirus Disease 2019 (COVID-19), operating through intersecting mechanisms of chronic inflammation, endothelial injury, and immunosuppression that collectively amplify viral pathogenicity. This study investigated the impact of diabetes type on COVID-19 symptom severity, hospitalization duration, and recovery time among patients in Nasiriyah City, southern Iraq — a region substantially underrepresented in the global DM–COVID-19 literature. A descriptive cross-sectional study was conducted across healthcare facilities in Nasiriyah City from 2023 to 2024. A total of 150 hospitalized adult patients carrying confirmed diagnoses of both COVID-19 and type …
Seaweed-Derived Bioactives: A Promising Source Of Novel Therapeutics, Aida H. Shobier, Sahar W.M. Hassan, Safaa A. Abdel Ghani
Seaweed-Derived Bioactives: A Promising Source Of Novel Therapeutics, Aida H. Shobier, Sahar W.M. Hassan, Safaa A. Abdel Ghani
Blue Economy
The marine environment provides bioactive natural products with unique structures that are absent in terrestrial compounds. This review aims to provide a thorough overview of key studies on bioactives derived from seaweeds. It focuses on their potential to address common diseases such as microbial infections, diabetes, oxidative stress, inflammation, viral infections, cancer, immune disorders, and Alzheimer's disease as well as their potential use in drug delivery systems. The bioactive compounds found in seaweeds belong to various chemical classes, with the largest proportion being polysaccharides, lipids, proteins, phenolics, pigments, terpenoids, alkaloids, and sterols. These compounds possess significant pharmacological potential. …
High-Throughput Robotic Ethanol Inhibition Assays For Engineered Thermophilic Biofuel Strains, Kevin He, Daniel Olson, Marybeth Maloney, Anthony Lanahan
High-Throughput Robotic Ethanol Inhibition Assays For Engineered Thermophilic Biofuel Strains, Kevin He, Daniel Olson, Marybeth Maloney, Anthony Lanahan
Wetterhahn Science Symposium Posters
Ethanol stress assays are commonly used to evaluate microbial tolerance, metabolic adaptation, and fermentation performance. However, manual liquid handling introduces variability across replicate wells and small-volume pipetting steps, limiting reproducibility and throughput. This study developed an automated OT-2 robotic workflow to generate replicated ethanol concentration gradients for high-throughput inhibition assays in engineered thermophilic biofuel strains. Kinetic plate-reader measurements were used to quantify ethanol-dependent growth responses under anaerobic fermentation conditions. The reasearch question is: How do engineered thermophilic biofuel strains differ in ethanol-dependent growth inhibition under anaerobic fermentation conditions, and can automated robotic assays improve the reproducibility of these measurements? Can …
Effect Of Spry Of Potassium Ratio And Cycocel On Physiological Traits Of Maize (Zea Mays L), Asmaa Mohammed Hussein, Eabdallah Yasin Ali
Effect Of Spry Of Potassium Ratio And Cycocel On Physiological Traits Of Maize (Zea Mays L), Asmaa Mohammed Hussein, Eabdallah Yasin Ali
AUIQ Complementary Biological System
The aim of this work was to investigating the effect of foliar application of potassium (K) and the growth regulator Cycocel (CCC) on the physiological traits of maize (Zea mays L). The experiment was designed using a split-plot arrangement within a randomized complete block design (RCBD) with three replicates. The treatments included foliar application of potassium at concentrations of 500, 1000, and 1500 mg.L–1, as well as three concentrations of the growth regulator Cycocel (0, 150, and 200 mg.L–1). The results indicated a significant increase in Protein (P), phosphorus, and carbohydrate contents at the concentration of …
Chronotropic Response To Intratracheal Versus Intravenous Atropine In Anesthetized Adult Patients: A Randomized Controlled Trial, Ammar H. Mahdi, Majid Alhamaidah, Myasar J. Mohammed, Hussein Ali Hussein, Hussein Alkhfaji
Chronotropic Response To Intratracheal Versus Intravenous Atropine In Anesthetized Adult Patients: A Randomized Controlled Trial, Ammar H. Mahdi, Majid Alhamaidah, Myasar J. Mohammed, Hussein Ali Hussein, Hussein Alkhfaji
AUIQ Complementary Biological System
Atropine is a commonly used anticholinergic agent in anesthetic practice, administered primarily to counteract bradycardia. Rapid treatment of intraoperative bradycardia is clinically critical, as delays may lead to hemodynamic compromise. This study aimed to compare the onset, magnitude, and duration of the chronotropic response to atropine administered via the intravenous (IV) versus intratracheal (IT) route in anesthetized adult patients. A prospective, single-blind, randomized controlled study was conducted on 60 adult patients classified as American Society of Anesthesiologists (ASA) physical status I-II, scheduled for elective peripheral surgery under general anesthesia. Patients were randomly allocated into three equal groups (n = 20 …
Scb252 Fundamentals Of Biotechniques Laboratory Manual, Na Xu, Hanqi Ye
Scb252 Fundamentals Of Biotechniques Laboratory Manual, Na Xu, Hanqi Ye
Open Educational Resources
This laboratory manual for SCB252: Fundamentals of Biotechniques provides an integrated, hands-on introduction to core concepts and methods in modern biological research using Drosophila melanogaster as a model organism. The manual emphasizes laboratory safety, research integrity, and responsible conduct of research, alongside foundational experimental skills. Students are guided through key biological techniques including fly husbandry, genetic crosses, mutant analysis, microscopy, DNA extraction, polymerase chain reaction (PCR), gel electrophoresis, sequencing technologies, and bioinformatics tools such as BLAST and genome databases. Advanced modules introduce gene structure annotation, RNA-seq data analysis, and primer design, while fluorescence microscopy and protein analysis techniques further expand …
Virus-Like Particle Peptoid Conjugate As A Triple-Negative Breast Cancer Targeting Delivery System, Ricky Zhu
Virus-Like Particle Peptoid Conjugate As A Triple-Negative Breast Cancer Targeting Delivery System, Ricky Zhu
Chemistry Theses
Triple-negative breast cancer (TNBC), an aggressive form of breast cancer, characterized by its lack of estrogen receptors (ER), progesterone receptors (PR), and human epidermal growth factor receptors (HER2) poses a therapeutic challenge as common treatment options are rendered ineffective. Here is a strategy to develop a drug delivery system utilizing poly-glycinated LC129 8, a TNBC targeting peptoid, covalently attached to HK97 virus-like particles (VLP) containing a C-terminal LPETG sequence via a sortase mediated reaction. Using the properties of the cage-like structure of VLPs and the TNBC specificity of the LC129-8, high doses of chemotherapy can be efficiently loaded into the …
Artificial Photosynthesis To Overcome Oxygen Deprivation During Respiratory Disease Crisis-A Mini Review, Devadass Jessy Mercy Ms, Harini Rm Ms, Agnishwar Girigoswami Prof, Koyeli Girigoswami Prof
Artificial Photosynthesis To Overcome Oxygen Deprivation During Respiratory Disease Crisis-A Mini Review, Devadass Jessy Mercy Ms, Harini Rm Ms, Agnishwar Girigoswami Prof, Koyeli Girigoswami Prof
Chulalongkorn Medical Journal
Respiratory diseases are mainly treated using several symptomatic treatments, supported by oxygen for the patients. During the Coronavirus disease 2019 (COVID-19) pandemic, oxygen concentrators were in high demand, and even after that, many patients lost their lives to this pandemic due to a shortage of oxygen cylinders. This crisis instigated many entrepreneurs to open oxygen plants, which needed a large scale set up. The articles were searched using Google Scholar, Scopus, and Web of Science using appropriate keywords to write this review article. In this review, we have summarized the need for oxygen that can be overcome using artificial photosynthesis. …
Thrombin-Induced Myofibroblast Differentiation Is Regulated By Myocardin And Novel Effector Genes In Ipf Fibroblasts, Christiana Okeke
Thrombin-Induced Myofibroblast Differentiation Is Regulated By Myocardin And Novel Effector Genes In Ipf Fibroblasts, Christiana Okeke
Biotechnology Theses
Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease characterized by fibroblast activation, excessive extracellular matrix (ECM) deposition, and irreversible respiratory decline. In this study, we investigated the role of myocardin and its downstream effectors, RGS5, DLGAP5, and TAGLN, in thrombin-induced myofibroblast differentiation and ECM remodeling using primary human IPF fibroblasts. Myocardin knockdown significantly attenuated thrombin-induced expression of profibrotic genes, including FN1, COL1A1, PAI-1, ACTA2, and CNN1, as well as the novel effectors RGS5, DLGAP5, and TAGLN, confirming myocardin as a central regulator of fibrotic signaling. Silencing RGS5 produced minimal effects on gene and protein expression despite minor downward …
The Risk Of Bpa In B Lymphocyte-Mediated Autoimmunity, Mario J. Palacios
The Risk Of Bpa In B Lymphocyte-Mediated Autoimmunity, Mario J. Palacios
Senior Honors Theses
Autoimmune disorders disproportionately affect females, a bias largely attributed to estrogen's modulation of B lymphocyte development and tolerance. Bisphenol A (BPA), a common plastic monomer, shows estrogenic activity and acts as an endocrine disruptor and epigenetic modifier. This thesis examines how BPA may increase autoimmune risk by mimicking estrogen such that it can excite estrogen receptors which rescue autoreactive B lymphocytes from apoptosis. While direct research linking BPA to B lymphocyte-mediated autoimmunity is limited, understanding BPA’s course of action in B lymphocytes is critical for addressing the rising prevalence of autoimmune diseases and its possible link to the increased and …
Developing Antibodies Against Galectin-3 And Galectin-9 To Target Endometriosis, Ashleigh Burger, Noah Farnsworth, Jared J. Reisnouer, Cheyenne Vue
Developing Antibodies Against Galectin-3 And Galectin-9 To Target Endometriosis, Ashleigh Burger, Noah Farnsworth, Jared J. Reisnouer, Cheyenne Vue
2026 Symposium
Endometriosis is a disease characterized by pelvic pain and the formation of endometrial-like tissue on the outside of the uterine lining. This condition affects nearly 10% of women across the globe and confirming whether someone has endometriosis requires visualization in surgery. While the specific causes of this disease remain unclear, the over-expression of the Galectins 3 and 9 in ectopic endometrial tissues suggests a role in the development and maintenance of the condition. Monoclonal antibodies (mAbs) are highly specific proteins with potential as diagnostic or treatment tools. The goal of this study is to generate antibodies against Gal3 and Gal9. …
Myocardin-Dependent Regulation Of Adam12 And Adam19 Promotes Fibronectin Fibrillogenesis During Pleural Fibrosis, Uday Siva Naga Sankar Allamneni
Myocardin-Dependent Regulation Of Adam12 And Adam19 Promotes Fibronectin Fibrillogenesis During Pleural Fibrosis, Uday Siva Naga Sankar Allamneni
Biotechnology Theses
Pleural fibrosis involves excessive extracellular matrix (ECM) deposition driven by chronic injury and inflammation. Transforming growth factor-β (TGF-β) induces mesothelial–mesenchymal transition (MesoMT), promoting a myofibroblast phenotype. Our laboratory identified myocardin (Myocd), a transcriptional coactivator, as a key mediator of MesoMT that regulates ECM-remodeling proteases. RNA-seq and qPCR analyses showed that ADAM12 and ADAM19 are upregulated by TGF-β but suppressed by Myocd knockdown in Human pleural mesothelial and IPF-derived cells. Silencing ADAM12 reduced fibrotic markers, including fibronectin and smooth muscle actin (α-SMA), whereas ADAM19 showed relatively minor effects. ADAM12 knockdown also impaired fibronectin fibrillogenesis and insoluble matrix assembly, as confirmed by …
Producing Oral Medicines In Lettuce To Fight Chronic Diseases, Carlos E. Balmaceda, Megha Goyal, Anubhav Pal
Producing Oral Medicines In Lettuce To Fight Chronic Diseases, Carlos E. Balmaceda, Megha Goyal, Anubhav Pal
Create@State
Chronic diseases, such as inflammatory bowel diseases, require patients’ commitment to their treatment, which can be challenging over time. Edible plants could help address this challenge by acting as living factories that produce a range of drugs, including medicinal proteins. Patients could eat the plants because their cells have a sturdy outer layer that protects and helps deliver the medicine. Lettuce is an example of a common vegetable that can serve this purpose. The main goal of this project is to develop a medicinal protein production and oral delivery system using lettuce plants. We will build a small version of …
Role Of Kif4a In The Fxa-Induced Phenotypic Modulation Of Pleural Mesothelial Cells, Sudishna Khanal
Role Of Kif4a In The Fxa-Induced Phenotypic Modulation Of Pleural Mesothelial Cells, Sudishna Khanal
Biotechnology Theses
Pleural injuries can disrupt the organization of the pleural surface, leading to pleural remodeling and fibrosis (PF), characterized by the proliferation of myofibroblasts expressing alpha-smooth muscle actin (α-SMA) and the deposition of extracellular matrix proteins, primarily due to the mesenchymal transition of pleural mesothelial cells (PMCs) (MesoMT). It is well established that various external stimuli, such as TGF-β, thrombin, and factor Xa (FXa), induce MesoMT. Our laboratory found that myocardin, a transcriptional co-activator, was dramatically up-regulated during MesoMT, and that gene silencing of myocardin (MyoCD) diminished MesoMT. RNA sequencing data showed that FXa activated a set of genes not upregulated …