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Insights Into Halophilic Microbial Adaptation: Analysis Of Integrons And Associated Genomic Structures And Characterization Of A Nitrilase In Hypersaline Environments, Sarah Sonbol 2021 American University in Cairo

Insights Into Halophilic Microbial Adaptation: Analysis Of Integrons And Associated Genomic Structures And Characterization Of A Nitrilase In Hypersaline Environments, Sarah Sonbol

Theses and Dissertations

Hypersaline environments are extreme habitats that can be exploited as biotechnological resources. Here, we characterized a nitrilase (NitraS-ATII) isolated from Atlantis II Deep brine pool. It showed higher thermal stability and heavy metal tolerance compared to a closely related nitrilase.

We also studied integrons in halophiles and hypersaline environments. Integrons are genetic platforms in which an integron integrase (IntI) mediates the excision and integration of gene cassettes at specific recombination sites. In order to search for integrons in halophiles and hypersaline metagenomes, we used a PCR-based approach, in addition to different bioinformatics tools, mainly IntegronFinder.

We found that integrons and ...


Investigating Local Adaptation To Hypoxia Stress In The Eastern Oyster Through Comparative Transcriptomics, Heather Nichole Smith 2021 Louisiana State University and Agricultural and Mechanical College

Investigating Local Adaptation To Hypoxia Stress In The Eastern Oyster Through Comparative Transcriptomics, Heather Nichole Smith

LSU Master's Theses

Climate change represents one of the most important challenges to biodiversity, therefore it is important to understand the mechanisms that allow species to respond to rapid environmental change. Here, we compared two populations of eastern oysters, Crassostrea virginica, from the Gulf of Mexico to study the mechanisms underlying hypoxia tolerance. Using a common garden experiment and comparative transcriptomics, we identified sets of genes involved in the hypoxia response and found differences in both the timing and baseline expression of hypoxia-responsive genes between tolerant and sensitive populations, consistent with a scenario of local adaptation. These genes include the signaling transcription factor ...


Impact Of Seawall Type On Mollusc Size And Diversity In South Water Caye Belize: A Case Study, Morgan O. Orand, Ginger R. Fisher 2021 University of Northern Colorado

Impact Of Seawall Type On Mollusc Size And Diversity In South Water Caye Belize: A Case Study, Morgan O. Orand, Ginger R. Fisher

Ursidae: The Undergraduate Research Journal at the University of Northern Colorado

The size and diversity of molluscs on and in front of artificial and natural sea walls in the tropical rocky intertidal of South Water Caye, Belize were investigated with the intent of determining the suitability of artificial surfaces to replace natural seawalls. Four seawalls, three artificial and one natural, were observed over a period of eight days. Mollusc size did not change based on distance from the wall for any of the walls. However, there were some significant differences in the species and number of molluscs in front of each wall as well as on the walls themselves. Measures of ...


Liner Sailing Speed Optimization Considering Wind And Waves, Zhaokun Wei, Xinlian Xie, Tiantian Bao 2021 College of Transportation, Shandong University of Science and Technology, Qingdao, China

Liner Sailing Speed Optimization Considering Wind And Waves, Zhaokun Wei, Xinlian Xie, Tiantian Bao

Journal of Marine Science and Technology

This paper proposes a new sailing speed optimization problem considering sea conditions such as wind and waves for a container shipping company. Since wind and waves, as exterior factors, can reduce speed, more power is employed as compensation to maintain regular service frequency and avoid delay, resulting in additional bunker consumption and higher cost. Hence, it is necessary to optimize the sailing speed according to the wind and waves. A power model was built to determine the reduced speed caused by the wind and waves for quantifying the speed reduction. On the basis of the proposed model, we developed a ...


A Bilevel Mathematical Approach For The Empty Container Repositioning Proble With A Sharing And Exchanging Strategy In Liner Shipping, Dung-Ying Lin, Chieh-Ju Juan 2021 Department of Industrial Engineering and Engineering Management, National Tsing Hua University, Hsinchu City, Taiwan

A Bilevel Mathematical Approach For The Empty Container Repositioning Proble With A Sharing And Exchanging Strategy In Liner Shipping, Dung-Ying Lin, Chieh-Ju Juan

Journal of Marine Science and Technology

In the maritime industry, the management of empty containers is a significant issue whose costs account for a large proportion of the total world fleet running costs. To reduce repositioning activities and increase cost effectiveness, this paper proposes a solution framework considering empty container sharing and exchanging strategies to coordinate the shipping lines in a maritime transportation network. The proposed solution framework considers an empty container matching platform company and a group of shipping lines in a given shipping network. Because of the interactive relationship between the two parties, the proposed problem is formulated as a bilevel programming problem, and ...


Investigating Model Solution Correctness For Parameter Uncertainty In Both Objective Function And Constraints, Shangyao Yan, Sin-Siang Wang, Chun-Yi Wang 2021 Department of Civil Engineering, National Central University, Taoyuan, Taiwan, R.O.C.

Investigating Model Solution Correctness For Parameter Uncertainty In Both Objective Function And Constraints, Shangyao Yan, Sin-Siang Wang, Chun-Yi Wang

Journal of Marine Science and Technology

To resolve engineering management problems encountered in the real world, optimization models are usually formulated with some parameter assumptions. Parameter uncertainty, which may arise due to changes in the environment or human error, may thus be incorporated into the objective function and the constraints. However, to simplify the modeling, the values of these parameters are usually set or projected as deterministic values. It is no wonder that the modelling results based on these inaccurate parameters are neither correct nor reliable. Thus, it is important to examine the correctness of the model results in relation to parameter uncertainty. This study aims ...


Modular Design And Implementation Of An Automatic Power Factor Regulator For Energy Savings On Fishing Vessels, Chien-Hsing Lee, Shih-Hsien Hsu, Joe-Air Jiang 2021 Department of Systems and Naval Mechatronic Engineering, National Cheng Kung University, Tainan, Taiwan

Modular Design And Implementation Of An Automatic Power Factor Regulator For Energy Savings On Fishing Vessels, Chien-Hsing Lee, Shih-Hsien Hsu, Joe-Air Jiang

Journal of Marine Science and Technology

This study describes the experiences of modular design and implementation of an automatic power factor regulator for improving energy efficiency on Taiwanese longline fishing vessels. The load capacities of such vessels are typically divided into three categories, depending on the gross tonnage or fish carrying capacity: less than 100 kW, from 100 kW to less than 250 kW, and larger than 250 kW. For attaining proper power factor improvement, a compensation unit with a total of 30 kVar, 60 kVar and 90 kVar was sized and assembled with capacitors of 10, 15 and 20 kVar to enable control within 12 ...


Key Risk Factors Influencing Harbor Tugboat Operations For Kaohsiung Port, Wen-Jui Tseng, Ji-Feng Ding, Chia-Ming Liu, Liou-Yuan Li 2021 Department of Shipping and Transportation Management, National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan, R.O.C.

Key Risk Factors Influencing Harbor Tugboat Operations For Kaohsiung Port, Wen-Jui Tseng, Ji-Feng Ding, Chia-Ming Liu, Liou-Yuan Li

Journal of Marine Science and Technology

The harbor tugboat operation is a very important business and process of Kaohsiung port. To ensure the safety of harbor tugboat operations and to reduce the occurrence of accidents, it is an important issue to conduct a risk assessment of tugboat operations for Kaohsiung port. Hence, the main purpose of this paper is employ the analytic hierarchy process (AHP) method to evaluate key risk factors influencing the harbor tugboat operations for Kaohsiung port. At first, a total of three evalu-ation dimensions with 14 preliminary risk factors are gener-ated from literature and interviews of tugboat captains. We then applied the steps ...


Free Vibration Analysis Of A Free-Free Single-Tapered Beam Carrying Arbitrary Concentrated Elements Using Modified Mode-Superposition Method (Mmsm), Chia-Chin Wu 2021 Department of Mechanical and Energy Engineering, National Chia-Yi University, Chia-Yi, Taiwan

Free Vibration Analysis Of A Free-Free Single-Tapered Beam Carrying Arbitrary Concentrated Elements Using Modified Mode-Superposition Method (Mmsm), Chia-Chin Wu

Journal of Marine Science and Technology

The literature contains scant information on the free vibration analyses of the loaded single-tapered beams (STBs) (i.e., STBs carrying concentrated elements (CEs)) Furthermore, even when such studies have been performed, they generally only consider the case of constrained (e.g., free-clamped (F-C)) beams. In other words, the problem of unconstrained (e.g., free-free (F-F)) loaded STBs has not yet to be addressed. Thus, the present study employs the modified mode-superposition method (MMSM) to investigate the free vibration characteristics of the F-F loaded STB carrying arbitrary various CEs. The investigation focuses particularly on the natural frequencies and mode shapes of ...


Optimum Design Of The Underwater Discharge System Based On Surrogate Modeling, Jisang Ha, Myung-Il Roh, Seung-Ho Ham, Sung-Jun Lee 2021 Department of Naval Architecture and Ocean Engineering, Seoul National University, Seoul, Republic of Korea

Optimum Design Of The Underwater Discharge System Based On Surrogate Modeling, Jisang Ha, Myung-Il Roh, Seung-Ho Ham, Sung-Jun Lee

Journal of Marine Science and Technology

Underwater vehicles are equipped with a device for discharging projectiles, called the underwater discharge system. Because these vehicles have limited space, many studies have attempted to reduce the volume of the underwater discharge system while improving its performance. However, owing to the characteristics of the system, the occupied volume and performance conflict with each other. Therefore, minimizing the occupied volume while satisfying the required performance criteria is the most significant requirement in the design stage. To address this, an optimization problem for the underwater discharge system was mathematically formulated and solved using multi-objective genetic algorithms. Additionally, we proposed a method ...


An Analysis Of Key Influencing Factors On Island Tourism Revisit Intentions – Evidence From The Matsu Islands, Chun-Chieh Wang, Hsiao-Chuan Liu, Hsuan-Shih Lee, Cheng-Chi Chung 2021 Dept. of Shipping and Transportation Management, National Taiwan Ocean University, Taiwan

An Analysis Of Key Influencing Factors On Island Tourism Revisit Intentions – Evidence From The Matsu Islands, Chun-Chieh Wang, Hsiao-Chuan Liu, Hsuan-Shih Lee, Cheng-Chi Chung

Journal of Marine Science and Technology

Sightseeing and tourism not only brings economic benefits to the local community but also drive the development of related industries. In 1992, when the Matsu’s battlefield mission was dissolved, tourism became one of the key development strategies to boom the region. Although the number of visitors to Matsu Islands has risen significantly in recent years, how to increase the island’s willingness to revisit the island has been an important issue. Since local tourism has its own characteristics, island tourism marketing strategies should be different. Many previous studies have quantified the relationship between tourism area satisfaction and willingness to ...


3d Numerical Simulation Of Kuroshio-Induced Wake Near Green Island, Taiwan, Tien-Hung Hou, Jen-Yi Chang, Chia-Cheng Tsai, Tai-Wen Hsu 2021 epartment of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan

3d Numerical Simulation Of Kuroshio-Induced Wake Near Green Island, Taiwan, Tien-Hung Hou, Jen-Yi Chang, Chia-Cheng Tsai, Tai-Wen Hsu

Journal of Marine Science and Technology

In this research, Kuroshio-induced vortex shedding in the lee of Green Island was numerically studied using the three-dimensional Princeton Ocean Model. The temporal and spatial characteristics of the vortex street were analyzed at different tidal levels, wind fields and water depths. The nu-merical results were validated by comparing with in-situ measurements from the Green Island tidal station, shipboard acoustic doppler current profiler (ADCP), conductivi-ty-temperature-depth (CTD) profiler, and moderate resolution imaging spectroradiometer (MODIS) satellite image datasets. Also, the three-dimensional results of velocity, temperature, and salinity fields were compared well with observational data. Furthermore, the influence depths of the wind effect and ...


Intelligent Fuzzy Control With State-Derivative Feedback For Takagi-Sugeno Fuzzy Stochastic Singular Systems, Wen-Jer Chang, Kuang-Yow Lian, Cheung-Chieh Ku, Che-Lun Su 2021 Department of Marine Engineering, National Taiwan Ocean University, Keelung, Taiwan, R. O. C.

Intelligent Fuzzy Control With State-Derivative Feedback For Takagi-Sugeno Fuzzy Stochastic Singular Systems, Wen-Jer Chang, Kuang-Yow Lian, Cheung-Chieh Ku, Che-Lun Su

Journal of Marine Science and Technology

This document deals with the problem of passive fuzzy controller design with the state-derivative feedback approach for the nonlinear stochastic singular systems. Recently, the singular systems have a greater focus on literature because they can keep more physical system characteristics than conventional systems. At first, the Takagi-Sugeno fuzzy stochastic singular models are used to represent the nonlinear stochastic singular systems. Then, the state-derivative feedback approach and parallel distributed compensation method are employed to design the passive fuzzy controllers. In the design process, the Lyapunov stability conditions are developed subject to multiple performance constraints, including the passivity constraint and decay rate ...


Sea Ice Warning Visualization And Path Planning For Ice Navigation Based On Radar Image Recognition, Tsung-Hsuan Hsieh, Shengzheng Wang, Huijia Gong, Wei Liu, Ning Xu 2021 Merchant Marine College, Shanghai Maritime University, Shanghai, China

Sea Ice Warning Visualization And Path Planning For Ice Navigation Based On Radar Image Recognition, Tsung-Hsuan Hsieh, Shengzheng Wang, Huijia Gong, Wei Liu, Ning Xu

Journal of Marine Science and Technology

Ice navigation is currently one of the important issues in path planning studies. Most studies are using sea ice models or ice charts for large-scale path planning, but the weather and ice conditions in the polar waters usually change quickly and are not easy to predict. Under these conditions, small-scale path planning that can be adjusted in time for sea ice collision avoidance is needed. This study uses marine radar imaging to reconstruct the ice navigation scene, and establishes a visual-ization function of sea ice warning on the radar image. We combine the bidirectional rapidly-exploring random tree (B-RRT) algorithm and ...


Enhancing Marine Administrative Management Based On Human Factor Through Safety Criteria, Wen-Cheng Lin, Hsin-Hung Cheng 2021 Department of Business Administration, National Taipei University of Business

Enhancing Marine Administrative Management Based On Human Factor Through Safety Criteria, Wen-Cheng Lin, Hsin-Hung Cheng

Journal of Marine Science and Technology

This study aims to improve maritime safety by enhancing administrative management based on human factors. This study used Fuzzy analytic hierarchy process to analyze the risks and threats regarding safety maritime criteria. Establishing administrative reform is given the highest priority. First, the current model for crew training system is explored. Second, the marine surveyor is examined to determine if it is professional enough for inspection work. Third, the investigation system used by maritime safety investigators should be inspected. The suggestions mentioned expect to establish a maritime administrative reform that could pave the way for the application of maritime safety around ...


Experimental Study On Treatment Of Ballast Water By Inert Flue Gas Deoxidation, Guozhi Bao, Peng Yang, Rui Da, Junxia Li, Guanghui Zhao, Ning Chen 2021 School of Energy and Power, Jiangsu University of Science and Technology, Zhenjiang, China

Experimental Study On Treatment Of Ballast Water By Inert Flue Gas Deoxidation, Guozhi Bao, Peng Yang, Rui Da, Junxia Li, Guanghui Zhao, Ning Chen

Journal of Marine Science and Technology

In this paper, the antimicrobial effect of inert flue gas on indicator microorganisms in ballast water was investigated based on a 265h test. The inert flue gas consists of nitrogen (N2) and carbon dioxide (CO2) the microorganisms include Chlorella and Escherichia coli. Within the scope of the test, the killing effect of the microbes treated by combining N2 and CO2 was higher than that of using only either N2 or CO2. When the dissolved oxygen concentration in the ballast water was 0.5 mg/L and the pH value was 6, the killing effect was the highest. At this time ...


Effects Of Salinity On Eastern Oysters: Locating Lower-Salinity Tolerant Populations And Defining Resource Zones Suitable To Restoration, Fisheries, And Aquaculture., Lauren Swam 2021 Louisiana State University and Agricultural and Mechanical College

Effects Of Salinity On Eastern Oysters: Locating Lower-Salinity Tolerant Populations And Defining Resource Zones Suitable To Restoration, Fisheries, And Aquaculture., Lauren Swam

LSU Master's Theses

Eastern oysters (Crassostrea virginica) provide valuable ecosystem services and support a productive commercial industry in the northern Gulf of Mexico. Declining abundance from water quality changes and other factors drives development of management and restoration strategies focused on a comprehensive, metapopulation approach. Identifying oyster resource zones based on water quality combined with selective breeding of oysters adapted to specific conditions provides strategies to support aquaculture development and ensure resilient oyster populations and high production. Using 2015-2019 satellite-derived continuous salinity and temperature data for coastal Louisiana, this work created maps defining oyster resource zones supportive of (1) broodstock sanctuary reefs, (2 ...


Deep-Sea Biodiversity At The Extremes Of The Salas Y Gómez And Nazca Ridges With Implications For Conservation, Alan M. Friedlander, Whitney Goodell, Jonatha Giddens, Erin E. Easton, Daniel Wagner 2021 The University of Texas Rio Grande Valley

Deep-Sea Biodiversity At The Extremes Of The Salas Y Gómez And Nazca Ridges With Implications For Conservation, Alan M. Friedlander, Whitney Goodell, Jonatha Giddens, Erin E. Easton, Daniel Wagner

Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

The Salas y Gómez and Nazca ridges are underwater mountain chains that stretch across 2,900 km in the southeastern Pacific and are recognized for their high biodiversity value and unique ecological characteristics. Explorations of deep-water ecosystems have been limited in this region, and elsewhere globally. To characterize community composition of mesophotic and deep-sea demersal fauna at seamounts in the region, we conducted expeditions to Rapa Nui (RN) and Salas y Gómez (SyG) islands in 2011 and Desventuradas Islands in 2013. Remote autonomous baited-cameras were used to conduct stationary video surveys between 150–1,850 m at RN/SyG (N ...


A Rapid Phenotype Change In The Pathogen Perkinsus Marinus Was Associated With A Historically Significant Marine Disease Emergence In The Eastern Oyster, Ryan Carnegie, Susan E. Ford, Rita K. Crockett, Peter R. Kingsley-Smith, Lydia M. Bienlien, Lucia S.L. Safi, Laura A. Whitefleet-Smith, Eugene M. Burreson 2021 Virginia Institute of Marine Science

A Rapid Phenotype Change In The Pathogen Perkinsus Marinus Was Associated With A Historically Significant Marine Disease Emergence In The Eastern Oyster, Ryan Carnegie, Susan E. Ford, Rita K. Crockett, Peter R. Kingsley-Smith, Lydia M. Bienlien, Lucia S.L. Safi, Laura A. Whitefleet-Smith, Eugene M. Burreson

VIMS Articles

The protozoan parasite Perkinsus marinus, which causes dermo disease in Crassostrea virginica, is one of the most ecologically important and economically destructive marine pathogens. The rapid and persistent intensification of dermo in the USA in the 1980s has long been enigmatic. Attributed originally to the effects of multi-year drought, climatic factors fail to fully explain the geographic extent of dermo’s intensification or the persistence of its intensified activity. Here we show that emergence of a unique, hypervirulent P. marinus phenotype was associated with the increase in prevalence and intensity of this disease and associated mortality. Retrospective histopathology of 8355 ...


Splitting The Scatter: Distinguishing Marine Organisms From Oceanographic Structures Using Acoustic Monitoring, Zhen Lu, Daisuke Hasegawa, Takahiro Tanaka, David M. Spencer, Yuji Okazaki, Takeshi Okunishi 2021 Fisheries Resources Institute, Japan Fisheries Research and Education Agency,Japan

Splitting The Scatter: Distinguishing Marine Organisms From Oceanographic Structures Using Acoustic Monitoring, Zhen Lu, Daisuke Hasegawa, Takahiro Tanaka, David M. Spencer, Yuji Okazaki, Takeshi Okunishi

Journal of Marine Science and Technology

In the northwest Pacific Ocean, off the Sanriku coast of Japan, convergence between the Tsugaru Warm Current (TWC) and the cold Oyashio Current (OC) results in the formation of highly dynamic oceanographic features that promote high biodiversity and generate hotspots for a range of commercially important fisheries. However, their effects on local biodiversity over much smaller spatiotemporal scales ( < 1 km) remains unclear. With the development of acoustic technology in recent years, echo-sounders are increasingly being used to conduct high-precision fishery resource assessments and detect small-scale variability in the oceanic thermohaline structure. In this study, we conducted a simultaneous hydrographic and dual-frequency echo-sounder survey to discriminate oceanographic features and marine organisms in the TWC-OC confluence zone. Echograms of acoustic backscatter measured at 38 kHz detected similar oceanographic features that were obtained from our hydrographic survey observations, but these features were less pronounced in the 120 kHz echograms. The dynamic oceanographic structure in the TWC-OC zone returned a large amount of backscatter, making it difficult to identify marine organisms from the scatter. The frequency differences of volume backscattering strength (SV) were, however, able to distinguish oceanographic structures from zooplankton detections. Outcomes from this study highlight the difficulty of distinguishing between hydrographic and biological detections in dynamic oceanographic environments. Further research into the acoustic frequency characteristics of heterogeneous hydrographic features will help better understand how species distributions are affected, so that the acoustic method can be used to provide more accurate estimates of species abundance.


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