Murray State University
University of Minnesota Morris Digital Well
University of Wisconsin Milwaukee
Bronx Community College
Washington University in St Louis
University of the Pacific
University of Arkansas, Fayetteville
Aga Khan University
University of Massachusetts Amherst
Gap Junctional Intercellular Communication: Role Of Cx43 Phosphorylation By Tyrosine Kinases,
2023
University of Nebraska Medical Center
Gap Junctional Intercellular Communication: Role Of Cx43 Phosphorylation By Tyrosine Kinases, Ishika Basu
Theses & Dissertations
Phosphorylation of Cx43 is a process that regulates various functionalities of the gap junction including assembly, stability, internalization, turnover, channel permeability and channel gating. Src has been shown to phosphorylate Cx43 at Y247, Y265, Y313 and regulate gap junctions. Intercellular communication in B and T cells is necessary to mediate adaptive immune response. However, there is little-to-no expression of Src in these lymphocytes. An in vitro kinase screen identified Bruton’s tyrosine kinase (BTK) and Interleukin 2-inducible T-cell kinase (ITK) to phosphorylate Cx43. Mass spectrometry identified Tyr residues to be phosphorylated by BTK and ITK, and these residues are similar to …
Analysis Of The Paraventricular Nucleus Of The Hypothalamus After Exposure To Decabromodiphenyl Ether In Mice,
2023
State University of New York College at Geneseo
Analysis Of The Paraventricular Nucleus Of The Hypothalamus After Exposure To Decabromodiphenyl Ether In Mice, Tia Blossomgame, Hermei Herman, Sharlenn La, Jocelyn Saquisili, Annabella Vargas, Grace Martinichio, Vincent P. Markowski
McNair Scholars Program
No abstract provided.
Amyloid-Beta Protein Concentration Dependence Of Reversible Aggregation Using Gold Colloid Particles,
2023
State University of New York College at Geneseo
Amyloid-Beta Protein Concentration Dependence Of Reversible Aggregation Using Gold Colloid Particles, Renee Spencer, Lila Kocieniewski, Bryan Martinez, Kazushige Yokoyama
McNair Scholars Program
Although Alzheimer’s and COVID-19 are different diseases, the commonality between them is during the process of them developing in a person by fibrillogenesis. The product of fibrillogenesis results in the development of these diseases. We study the first stage of fibrillogenesis where the amyloid-beta peptide monomers are assembled into an oligomer. We want to isolate this oligomer using gold colloids because this step can be reversed. Utilizing gold colloids allows us to freeze fibrillogenesis in the first step by folding and unfolding the protein repeatedly through a series of pH changes from 4 or below to 10 or higher. At …
Biochemical Markers Of Liver Toxicity Among Coal Mine Workers Of Punjab, Pakistan Suffering From Hcv,
2023
Department of Zoology, University of Sargodha, Pakistan
Biochemical Markers Of Liver Toxicity Among Coal Mine Workers Of Punjab, Pakistan Suffering From Hcv, Aima Iram Batool, Asma Noreen, Naima Huma Naveed, Iram Inayat, Muhammad Fayyaz Ur Rehman, Fariha Idrees, Muhammad Ali Kanwal, Syeda Humaira Jabeen, Hakim Bibi
Journal of Bioresource Management
Hepatitis C is among the leading hepatic disorders in current period through which about 3 % world population has been anguish among them 170 million were diagnosed as persistent carriers. A great range of alteration in liver biochemical parameters were found to be allied with HCV infestation. Current study was designed to evaluate the extent of HCV mediated abnormalities in liver biochemical markers which includes ALT (alanine aminotransferase), AST (Aspartate transaminase), ALP (Alkaline Phosphatase) and serum bilirubin. The study was conducted on coal miners of Punjab province, Pakistan. HCV was primarily diagnosed through one step rapid test device after which …
Raw Diffraction Data For 3tel-Hlx-Darpin (Sumo), Wwpdb Id: 7n2b,
2023
Brigham Young University - Provo
Raw Diffraction Data For 3tel-Hlx-Darpin (Sumo), Wwpdb Id: 7n2b, Supeshala D. Nawarathnage, Parag Laljibhai Gajjar, Derick R. Bunn, Cameron Stewart, Tzanko I. Doukov, James Moody
ScholarsArchive Data
This X-ray diffraction dataset was collected on October 21Oct2020 at SSRL Beamline BL9-2. The crystal was of 3TEL-hlx-DARPin (SUMO) The solved structure used frames 1-1799 and has been deposited in the wwPDB under the PDB ID 7N2B. This data can be readily processed using XDS, AutoProc, CCP4, HKL2000/3000, or other crystallographic X-ray diffraction data processing software. The files have a human-readable header but are otherwise binary.
Raw Diffraction Data For 1tel-Aa-Vwa (Sumo), Wwpdb Id: 7n1o,
2023
Brigham Young University - Provo
Raw Diffraction Data For 1tel-Aa-Vwa (Sumo), Wwpdb Id: 7n1o, Moriah H. Mathis, Braydan D. Bezzant, Diana T. Ramírez, Supeshala D. Nawarathnage, Tzanko I. Doukov, James Moody
ScholarsArchive Data
This X-ray diffraction dataset was collected on October 21Oct2020 at SSRL Beamline BL9-2. The crystal was of Batch 1 1TEL-AA-vWa (SUMO) The solved structure used frames 1-999 and has been deposited in the wwPDB under the PDB ID 7N1O. This data can be readily processed using XDS, AutoProc, CCP4, HKL2000/3000, or other crystallographic X-ray diffraction data processing software. The files have a human-readable header but are otherwise binary.
Investigation Into The Enhanced Activation Of The Are And The Correlations Between Nrf2 And Other Cytoprotective Pathways Such As Ahr, Hsp, And Apoptosis,
2023
Liberty University
Investigation Into The Enhanced Activation Of The Are And The Correlations Between Nrf2 And Other Cytoprotective Pathways Such As Ahr, Hsp, And Apoptosis, Jonathon Joseph Odom
Doctoral Dissertations and Projects
Oxidative damage is a cellular threat that is responsible for many pathologic conditions such as Alzheimer’s disease, Parkinson’s disease, chronic obstructive pulmonary disease (COPD), cancer, and many more. The primary cytoprotective pathway within the cell that is responsible for mitigating the harmful effects of oxidative stress is the antioxidant system. The master regulator of the antioxidant system is the transcription factor, nuclear factor erythroid 2-related factor 2 (NRF2) which operates by binding to the antioxidant response element (ARE) and inducing the production of many antioxidant genes. It is known that the early response of NRF2 is regulated by electrophilic interaction …
Mitocans Induce Lipid Flip-Flop And Permeabilize The Membrane To Signal Apoptosis,
2023
University of Windsor
Mitocans Induce Lipid Flip-Flop And Permeabilize The Membrane To Signal Apoptosis, Stuart R. Castillo, Michael H.L. Nguyen, Mitchell Dipasquale, Elizabeth G. Kelley, Drew Marquardt
Chemistry and Biochemistry Publications
Pancratistatin (PST) and narciclasine (NRC) are natural therapeutic agents that exhibit specificity toward the mitochondria of cancerous cells and initiate apoptosis. Unlike traditional cancer therapeutic agents, PST and NRC are effective, targeted, and have limited adverse effects on neighboring healthy, noncancerous cells. Currently, the mechanistic pathway of action for PST and NRC remains elusive, which in part inhibits PST and NRC from becoming efficacious therapeutic alternatives. Herein, we use neutron and x-ray scattering in combination with calcein leakage assays to characterize the effects of PST, NRC, and tamoxifen (TAM) on a biomimetic model membrane. We report an increase in lipid …
Metabolic Reprogramming In Kras-Driven Cancer Cells,
2023
The Graduate Center, City University of New York
Metabolic Reprogramming In Kras-Driven Cancer Cells, Ahmet Hatipoglu
Dissertations, Theses, and Capstone Projects
Cancer cells require nutrient uptake for anabolic reactions needed for cellular growth and proliferation. The mammalian target of rapamycin (mTOR) is a key regulator of cellular growth that links nutrient availability and growth factor signals. mTOR mediated signaling pathways are often dysregulated in a wide range of cancers. We previously reported that KRas-driven cancer cells sense exogenous lipids via de novo production of phosphatidic acid, which ultimately activates both mTOR complexes – mTORC1 and mTORC2. Activated mTORC2 phosphorylates the survival kinase Akt at Ser 473 which is required for full Akt activation. A direct substrate of Akt and a major …
The Development Of Novel Radioimmunoconjugates For The Pet Imaging And Radioimmunotherapy Of Cancer,
2023
The Graduate Center, City University of New York
The Development Of Novel Radioimmunoconjugates For The Pet Imaging And Radioimmunotherapy Of Cancer, Samantha M. Sarrett
Dissertations, Theses, and Capstone Projects
Antibodies have long played a vital role in nuclear medicine for both the diagnosis and therapy of various malignancies. The role and development of antibodies in nuclear medicine can be broadly separated into three different categories: 1) bioconjugation strategies, 2) immunoPET imaging, and 3) radioimmunotherapy. This dissertation will attempt to comprehensively cover each of these categories through a series of studies, protocols, and reviews. For the bioconjugation strategies, we will describe the development of a novel site-selective bioconjugation strategy using an innovative lysine-targeting reagent, PFP-bisN3, to prepare [89Zr]Zr-SSKDFO-pertuzumab for visualizing HER2+ breast cancer. Further, …
Extraction Of Challenging Forensic Samples Using The Microgem Dna Extraction Kit,
2023
CUNY John Jay College
Extraction Of Challenging Forensic Samples Using The Microgem Dna Extraction Kit, Falyn R. Vega
Student Theses
In forensic science, DNA extraction can be a tedious and resource-intensive process. Extraction with Proteinase K is an industry standard but has its drawbacks, such as requiring multiple ionic detergents and washing steps. MicroGEM has developed a new enzyme called forensicGEM that is temperature-dependent and compatible with mesophilic enzymes, offering complete DNA extraction in about 20 minutes in a single tube, limiting contamination, loss of sample, and working time. ForensicGEM can extract DNA from highly degraded samples, potentially leading to more complete STR profiles. Highly degraded tissue and bone samples were collected and extracted with the forensicGEM …
Role Of Chronic Stress-Induced Neuroinflammation In Rodent Locus Coeruleus Physiology And Anxiety-Like Behaviors,
2023
Rowan University
Role Of Chronic Stress-Induced Neuroinflammation In Rodent Locus Coeruleus Physiology And Anxiety-Like Behaviors, Arthur Anthony A. Reyes
Graduate School of Biomedical Sciences Theses and Dissertations
The locus coeruleus (LC), the primary site of brain norepinephrine (NE), is a key anatomical brain region implicated in the stress response. Stress is a neuroendocrine physiologic response to a stressor that promotes organism survival through adaptive change and restoration of homeostasis. The central stress response, which drives behavioral and physiological change, is primarily mediated by activating the hypothalamic-pituitary-adrenal (HPA) axis. While advantageous in the short term, chronic stress exposure can lead to HPA axis and LC dysregulation, which are thought to contribute to the etiology of anxiety disorders. Previous studies demonstrate the effects of acute stress in increasing LC …
First Course Portfolio For Bioc205: Scientific Analysis And Technical Writing,
2023
University of Nebraska - Lincoln
First Course Portfolio For Bioc205: Scientific Analysis And Technical Writing, Lindsey B. Crawford
UNL Faculty Course Portfolios
BIOC205: Scientific Analysis and Technical Writing is a core course for the Department of Biochemistry at the University of Nebraska-Lincoln. This course is geared for second year (sophomore students) with minimal prerequisites and as a foundation for future classes. Challenges include 1) teaching the needed scientific background and elements of scientific analysis and writing to a broad cohort of students with diverse backgrounds and interests, 2) building student skills towards analysis of primary peer-reviewed scientific literature, 3) developing students as writers of scientific information for different audiences. This course used Genetic Engineering as a broad topic choice to capture students …
Boron Neutron Capture Therapy In The New Age Of Accelerator-Based Neutron Production And Preliminary Progress In Canada,
2023
University of Windsor
Boron Neutron Capture Therapy In The New Age Of Accelerator-Based Neutron Production And Preliminary Progress In Canada, Dominik Dziura, Sana Tabbassum, Amanda Macneil, Dalini D. Maharaj, Robert Laxdal, Oliver Kester, Ming Pan, Hiroaki Kumada, Drew Marquardt
Chemistry and Biochemistry Publications
Each year more than 3000 Canadians are diagnosed with brain cancers like glioblastoma multiforme or recurrent head and neck cancers, which are difficult to treat with conventional radiotherapy techniques. One of the most clinically promising treatments for these cancers is boron neutron capture therapy (BNCT). This procedure involves selectively introducing a boron delivery agent into tumor cells and irradiating them with a neutron beam, which kills the cancer cells due to the high-linear energy transfer radiation produced by the 10B(n,α)7Li capture reaction. The theory of BNCT has been around for a long time since 1936, but has historically been limited …
Molecular Mechanisms Protecting Centromeres From Self-Sabotage And Implications For Cancer Therapy,
2023
Thomas Jefferson University
Molecular Mechanisms Protecting Centromeres From Self-Sabotage And Implications For Cancer Therapy, Rim Nassar, Lily Thompson, Elise Fouquerel
Student Papers, Posters & Projects
Centromeres play a crucial role in DNA segregation by mediating the cohesion and separation of sister chromatids during cell division. Centromere dysfunction, breakage or compromised centromeric integrity can generate aneuploidies and chromosomal instability, which are cellular features associated with cancer initiation and progression. Maintaining centromere integrity is thus essential for genome stability. However, the centromere itself is prone to DNA breaks, likely due to its intrinsically fragile nature. Centromeres are complex genomic loci that are composed of highly repetitive DNA sequences and secondary structures and require the recruitment and homeostasis of a centromere-associated protein network. The molecular mechanisms engaged to …
Control Of The Electroporation Efficiency Of Nanosecond Pulses By Swinging The Electric Field Vector Direction,
2023
Old Dominion University
Control Of The Electroporation Efficiency Of Nanosecond Pulses By Swinging The Electric Field Vector Direction, Vitalii Kim, Iurii Semenov, Allen S. Kiester, Mark A. Keppler, Bennett L. Ibey, Joel N. Bixler, Ruben M. L. Colunga Biancatelli, Andrei G. Pakhomov
Bioelectrics Publications
Reversing the pulse polarity, i.e., changing the electric field direction by 180°, inhibits electroporation and electrostimulation by nanosecond electric pulses (nsEPs). This feature, known as “bipolar cancellation,” enables selective remote targeting with nsEPs and reduces the neuromuscular side effects of ablation therapies. We analyzed the biophysical mechanisms and measured how cancellation weakens and is replaced by facilitation when nsEPs are applied from different directions at angles from 0 to 180°. Monolayers of endothelial cells were electroporated by a train of five pulses (600 ns) or five paired pulses (600 + 600 ns) applied at 1 Hz or 833 kHz. Reversing …
Metals And Metal Complexes In Diseases With A Focus On Covid-19: Facts And Opinions,
2023
The John Paul II Catholic University of Lublin
Metals And Metal Complexes In Diseases With A Focus On Covid-19: Facts And Opinions, Agnieszka Ścibior, Manuel Aureliano, Alvin A. Holder, Juan Llopis
Chemistry & Biochemistry Faculty Publications
In the present Special Issue on “Metals and Metal Complexes in Diseases with a Focus on COVID-19: Facts and Opinions”, an attempt has been made to include reports updating our knowledge of elements considered to be potential candidates for therapeutic applications and certain metal-containing species, which are extensively being examined towards their potential biomedical use due to their specific physicochemical properties. The Special Issue compiles data on the role of metals in COVID-19 and focuses on other illnesses and biological processes that affect metal metabolism. It consists of eight manuscripts, including five review articles and three original research papers (Figure …
Bola3 And Nfu1 Link Mitoribosome Iron-Sulfur Cluster Assembly To Multiple Mitochondrial Dysfunctions Syndrome,
2023
University of Miami Miller School of Medicine
Bola3 And Nfu1 Link Mitoribosome Iron-Sulfur Cluster Assembly To Multiple Mitochondrial Dysfunctions Syndrome, Hui Zhong, Alexandre Janer, Oleh Khalimonchuk, Hana Antonicka, Eric Shoubridge, Antoni Barrientos
Biochemistry -- Faculty Publications
The human mitochondrial ribosome contains three [2Fe-2S] clusters whose assembly pathway, role, and implications for mitochondrial and metabolic diseases are unknown. Here, structure-function correlation studies show that the clusters play a structural role during mitoribosome assembly. To uncover the assembly pathway, we have examined the effect of silencing the expression of Fe-S cluster biosynthetic and delivery factors on mitoribosome stability. We find that the mitoribosome receives its [2Fe-2S] clusters from the GLRX5-BOLA3 node. Additionally, the assembly of the small subunit depends on the mitoribosome biogenesis factor METTL17, recently reported containing a [4Fe-4S] cluster, which we propose is inserted via the …
Silica Concentrations In Dominant Vegetation Species Of Wax Lake Delta, Louisiana,
2023
Louisiana State University and Agricultural and Mechanical College
Silica Concentrations In Dominant Vegetation Species Of Wax Lake Delta, Louisiana, Danielle M. Soileau
LSU Master's Theses
Coastal deltaic wetlands colonize dynamic environments and experience fluctuations of salinity, river connectivity, sediment dredging or deposition, and periods of inundation resulting in unique vegetation and soils. Louisiana’s coastline is experiencing enhanced loss of coastal deltaic wetlands in coastal basins where the Mississippi River no longer is connected to waterways and channels that would normally provide freshwater and sediments representing the abandonment phase of the delta cycle. There is a coastal basin in the central regions of the state, the Atchafalaya Bay, that is still in the active phase of delta cycle resulting in the growth of the Wax Lake, …
Migratory Material: Epigenetics & Weaving At The Us-Mexico Border,
2023
Rhode Island School of Design
Migratory Material: Epigenetics & Weaving At The Us-Mexico Border, Valerie Navarrete
Masters Theses
Discourse often sutures the body shut, disallowing representations of identity to outgrow sociopolitical interests. This issue may originate from borders, but also from the unnamable pathology that generational colonial trauma transmits to the mind, body, and environment. Without a direct form of translatability, this thesis proposes a new materialism that deviates from any object-oriented ontology. Untethered and intra-active, epigenetics and weaving represent objects that transform typical ways of knowing and seeing. Their sensitivity to the environment, in addition to their mobility across generations of time, broaden the spatiotemporal loci of the body and its embodiment. Proposing new materials that expand …
