Biotechnology Commons^™

Developing Herbal-Based Beverage Fermentation Using Saccharomyces Cerevisiae: The Physico-Chemical Properties, Siti Madihah Mohd Don, Mas Munira Rambli, Beston Faiek Nore

ASEAN Journal on Science and Technology for Development

Herbal-based fermented beverages are popular non-dairy products among consumers who seek healthy and immunity-supporting products. Amongst commonly used health supplements that are added to food and beverage would be probiotics, commonly Saccharomyces cerevisiae or yeast. This study aims to determine the physico-chemical properties of fermented beverages at different fermentation periods using lemongrass (Cymbopogon citratus DC. Stapf), ginger (Zingiber officinale Rosc.), turmeric (Curcuma longa L.), mint (Mentha), and moringa (Moringa oleifera Lam.). This study revealed a significant decrease (p ≤ 0.05) in pH, total soluble solids, and density in most fermented beverages after 24, 36 and 48 hours of fermentation. Low …

Exploring The Impact Of Vented Packaging Configurations On Airflow Patterns And Cooling Consistency For Muskmelons In A Cold Storage Environment, Nur Wafidah Roslan, Namasivayam Navaranjan, Zohrah Sulaiman, Naseem Uddin

ASEAN Journal on Science and Technology for Development

The cooling effect of different vented packaging designs was investigated for packed muskmelons fruits in a commercial cold storage condition. Computational fluid dynamics (CFD) simulation and experiments were used. The airflow pattern, heat transfer and temperature profiles were predicted from simulation and analysed. The CFD results reveal an uneven and low efficient airflow distribution inside a commercially used vented packaging (Box A). This resulted slow cooling of the fruits in Box A. Two newly designed packaging (Box B and Box C) showed better airflow distribution around the fruits and cooled them faster than Box A. The design of Box C …

Physicochemical Characterization Of Edible Insect Oils: Insights Into Fatty Acid Composition, Thermal Behavior And Quality Parameters, Eng-Tong Phuah, Yee-Ying Lee, Teck-Kim Tang, Guanghui Li, Shyang-Pei Hong, Syazana Abdullah Lim

ASEAN Journal on Science and Technology for Development

Edible insects are emerging as a sustainable food source due to their high nutritional value. This study employed the three-phase partitioning (TPP) method to extract oils from sago worm (Rhynchophorus ferrugineus), superworm (Zophobas morio), and cricket (Acheta domesticus). The study evaluated and compared the physicochemical properties of these insect oils, including iodine value (IV), peroxide value (PV), p-anisidine value (p-AV), acid value (AV), refractive index (RI), thermal behavior, and fatty acid compositions. Results revealed that the sago worm exhibited the highest oil yield (47.8 ± 2.2%) compared to the superworm (23.8 ± 1.4%) and cricket (6.4 ± 0.6%). Cricket oil …

Evaluation Of Physicochemical Properties Of Chicken Sausages Containing Whey Protein Isolate And Guar Gum, Nur Hamizah Hamid, Namasivayam Navaranjan

ASEAN Journal on Science and Technology for Development

The present work aims to evaluate the physicochemical properties and proximate composition of adding whey protein isolate (WPI) and guar gum (GG) as emulsion gels in chicken sausages formulated with different fat percentages. The fat reduction resulted in products having lower moisture content (p

Unlocking Antioxidant Potential: Comparative Analysis Of Sembada 188 And Keladi Rice As Functional Ingredients In Novel Food Production, Mas Munira Rambli, Natasha Azamain, Eng-Tong Phuah

ASEAN Journal on Science and Technology for Development

Rice (Oryza sativa L.) is an essential crop that is a dietary staple for more than half of the world's population. There is currently a movement in consumer behaviour toward healthier food consumption, which is fuelling interest in functional diets like coloured rice. Sembada 188 and Keladi rice are underutilised in Brunei Darussalam and are expected to have functional qualities. A comparison of antioxidant properties between Sembada 188 and Keladi rice was performed in this study. This research produced puffed rice treats prepared by steaming rice, washing, drying for 6-8 hours at 60℃, deep frying for 25 seconds at 180℃, …

Preliminary Study On The Effects Of Vinegar As Pre- Treatment For The Oven-Drying Of Pacific Yellowtail Emperor (Lethrinus Atkinsoni) Fillets, Nurisa A. Suhaili, Rafael S. Jamih, Normina A. Abubakar, Jaro O. Ajik, Merilyn Q. Amlani

ASEAN Journal on Science and Technology for Development

This study aimed to assess the impact of various vinegar compositions used as pre-treatment for Pacific Yellowtail Emperor (PYE) fillets during the oven-drying process, with a focus on moisture content and optimal drying conditions. Two types of commercially available vinegar, Superior Vinegar and Datu Puti Vinegar, were compared, and different drying temperatures were evaluated. The investigation revealed that the drying temperature significantly influenced the moisture content of the dried PYE fillets. Among the tested temperatures (40°C, 60°C, and 80°C), the most favorable outcome in terms of moisture content was achieved when fillets were dried at 80°C for a duration of …

Assessing The Organic Acids-Solubilized Collagen Derived From The Skin Of Unicornfish (Naso Reticulatus Randall, 2011) On Physicochemical And Structural Characteristics: Toward Alternative Source For Industrial Applications, Dayang Fazrianie Harianto, Abdul Aziz Jaziri, Rossita Shapawi, Ruzaidi Azli Mohd Mokhtar, Wan Norhana Md. Noordin, Masmunira Rambli, Nurul Huda

ASEAN Journal on Science and Technology for Development

Converting skin by-product of unicornfish (Naso reticulatus Randall, 2011) into a valuable commodity like collagen is a good strategy to explore alternative sources for land-based animals. Additionally, marine fish collagens are preferable due to their safer and great properties. This project aimed to compare extractability of collagens prepared with three organic acids (i.e., acetic, lactic and citric acids), and their characteristics were also discussed. Collagen extracted with citric acid (CSCUS) (7.10%) had a significantly higher yield (p

Byproducts Of Tropical Marine Processing As A Candidate For Industrial Food Ingredients: An Appraisal On Macro And Micronutrients, Abdul Aziz Jaziri, Hajariah Hasanuddin, Rossita Shapawi, Ruzaidi Azli Mohd Mokhtar, Wan Norhana Md. Noordin, Masmunira Rambli, Nurul Huda

ASEAN Journal on Science and Technology for Development

Tropical marine fish is a main raw material for surimi processing plants, including golden threadfin bream (Nemipterus virgatus Houttuyn, 1782) and swallowtail dwarf monocle bream (Parascolopsis eriomma Jordan & Richardson, 1909). After processing, more than 60% of fish are considered as byproducts (i.e., head, viscera, skin, bones, fins and scale), and tend to discard due to poor-utilization. Hence, this paper aims to evaluate macro- and micro-nutrients derived from golden threadfin bream (GTB) and swallowtail dwarf monocle bream (SDMB). The higher content of protein (25.31 ± 0.81%) was observed in the skin part of GTB, followed by the flesh (21.26 ± …

Kinetic Modelling During Soaking Of Dried Long Grain Paddy Mr297, Jhauharotul Muchlisyiyah, Rosnah Shamsudin, Roseliza Kadir Basha, Radhiah Shukri, Syahmeer How, Masmunira Rambli

ASEAN Journal on Science and Technology for Development

This study aimed to assess the soaking behaviour of MR 297 dried paddy under two conditions: room temperature and hot water soaking at 60°C. The experiment involved soaking dried MR297 paddy samples in water for 0.5 to 8 hours at room temperature and 0.5 to 5 hours for soaking at 60°C. The water absorption of MR 297 behaved differently at two different soaking conditions. The kinetic behaviour of the soaking process was also simulated using established 25 predictive models and incorporating 5 novel models presented in this work. The comparison of all prediction models involved using three statistical measures: the …

Formulation Of Naturally Flavoured Mineral Water With Curcuma Longa, Zumirra Affno, Aida M. Basri, Beston F. Nore

ASEAN Journal on Science and Technology for Development

Natural, organic flavoured beverages have become an increasingly important market in the food industry due to flavour and sensory requirements among consumers. Throughout human history, herbs and spices provide a spectrum of natural food flavouring, which became an eminent component of food preparations worldwide. However, their applications in beverages have not been fully exploited. The main objective of this study is to develop flavoured water formulation based on organic turmeric extracts from flower, leaf, and rhizome parts. We adhered the natural mineral water flavouring integrity at refrigerated storage condition (4oC) for a period of 12 weeks. The flavoured water was …

Development Of Halalan Tayyiban Plant-Based Cheese Formulations, Hasna B. Mazalana, Mas M. Rambli, Syazana A. Lim, Beston F. Nore

ASEAN Journal on Science and Technology for Development

The worldwide cheese production is dominated by animal milk sources with various industrial procedures, including animal rennet. There is a need to diversify the animal-based dairy products into plant-based one, not only to nurture the halalan tayyiban concept for Muslims, but also to accommodate the dietary restriction of some consumers. The objective of this work is to formulate a cheese type derived from plant-based milk with permissible ingredients to create a product for Muslim and non-Muslim consumers. In this study, we explored the use of conventional and non-conventional cheese-making procedures. A total of four experimental formulations (EF1-4) combinations were conducted …

Unveiling The Antioxidant Properties Of Stenochlaena Palustris And Diplazium Esculentum Through Time-Dependent Analysis, Nurnajiihah A. Samad, Hasna B. Mazalan, Hazimah Sharifulazar, Aida M. Basri, Masayoshi Arai

ASEAN Journal on Science and Technology for Development

Medicinal plants are generally known for its health benefits and there has been ongoing research to gather evidence on potential plants that could exhibit antioxidant activities. In this study, we explored underutilised local medicinal plants which are Stenochlaena palustris and Diplazium esculentum, locally known as ‘Lemiding’ and ‘Pakis’, respectively. The aim was to determine the effect of extraction time on phytochemical contents, antioxidant activities and total phenolic content (TPC) of the two plant species. Maceration method was used to extract the compounds from leaves of the plant in distilled water at different durations of extraction i.e. 2 hours, 4 hours, …

Contemporary Vs. Traditional Dna-Based Mutation Testing To Detect Hereditary Breast And Ovarian Cancer Syndrome (Hboc) In Women: A Meta Narrative Review, Hannah Olivia Cantu, Jacob Hipp, Nyela Y. Lopez, Duc Duy Nguyen

Research Methods Poster Session 2024

No abstract provided.

Reducing Food Scarcity: The Benefits Of Urban Farming, S.A. Claudell, Emilio Mejia

Journal of Nonprofit Innovation

Urban farming can enhance the lives of communities and help reduce food scarcity. This paper presents a conceptual prototype of an efficient urban farming community that can be scaled for a single apartment building or an entire community across all global geoeconomics regions, including densely populated cities and rural, developing towns and communities. When deployed in coordination with smart crop choices, local farm support, and efficient transportation then the result isn’t just sustainability, but also increasing fresh produce accessibility, optimizing nutritional value, eliminating the use of ‘forever chemicals’, reducing transportation costs, and fostering global environmental benefits.

Imagine Doris, who is …

Comprehensive Assessment Of Economic, Productive, Phenotypic, And Morphometric Traits In Azikheli Buffalo Breed Of Northern Pakistan, Nadar Khan, Bakhtawara Bibi, Tariq Ahmad, Salman Khan, Aamir Khan, Muhammad Ibrahim

Journal of Bioresource Management

The study was conducted to illuminate the phenotypic, morphometric, and productive characteristics of the Azikheli buffalo breed. One thousand two hundred pure Azikheli buffaloes (male: female) were randomly selected from the main hub of the Azikheli buffalo breed in Swat (Azikhel). The data were analyzed for comparison and frequency distribution. Phenotypic characteristic of the female buffalo shows that more than 73 % of the body color was golden yellowish. In contrast, in males, this value was 83 %, followed by white-albino and grayish colors. The shape of horns were semi sickle and grayish in color. Morphometric parameters of the breed …

Enzymatic Screening And Genotypic Characterization Of Thermophilic Bacteria From The Hot Springs Of Sarawak, Malaysia, Seng Chiew Toh, Samuel Lihan, Sui Sien Leong, Azizul Hakim Lahuri, Wai Cheong Woon, Wing Woh Ng

Makara Journal of Science

Owing to their eccentric thermostable ability, thermophiles are among the most utilized extremophiles in various industries, such as manufacturing, and clinical research. Researchers believe that many unknown thermophiles are yet to be discovered. This study aimed to genotypically characterize the diversity of thermophiles and screen them for the potential production of enzymes in the recreational hot springs located at Northwest Coast of Borneo. Water samples were collected at 45 °C–50 °C from Annah Rais and Panchor hot springs during the sampling period from January 2018 to January 2019. Three samples (water and sediment) were collected twice in a 3-week interval …

Using In Silico Tools To Analyze The 5ʹ Untranslated Regions Of The Alcohol Dehydrogenase Gene From Arabidopsis Thaliana And Omega Sequence, Reza Mohammadhassan, Tina Asadishad

Makara Journal of Science

The 5ʹ ends of protein-encoding genes contain 5ʹ untranslated regions (5ʹUTRs), which can effectively participate in regulating gene expression. The 5ʹUTRs of Arabidospis thaliana–derived alcohol dehydrogenase gene (AtADH) and omega (Ω) sequence from tobacco mosaic virus (TMV) are the most effective enhancers in biotechnology. In this study, bioinformatics techniques were employed to analyze the characteristics of the above sequences. After 5ʹUTR sequence collection, the inner ribosome entrance sites; small RNA (sRNA) target sequences; nucleotide contents; and upstream start and stop codons, ORFs, and coding DNA sequences of the Ω sequence and AtADH 5ʹUTR were identified. Moreover, the …

The Potential Of Endophytic Bacteria As The Biocontrol Agents Of Fusarium Proliferatum, Rima Ernia, Nisa Rachmania Mubarik, Lisdar Idwan Sudirman

Makara Journal of Science

Fusarium proliferatum decreases the productivity of shallot (Allium cepa L.). Endophytic bacteria are potential biocontrol agents in controlling the growth of F. proliferatum. This study aimed to select and characterize endophytic bacteria producing bioactive compounds that inhibit the growth of F. proliferatum. The endophytic bacteria used were isolated from the roots, stems, leaves, and soil of shallot plants from healthy and diseased shallot farming fields. The endophytic bacteria were selected using the dual culture method and extracted using ethyl acetate solvent. The results of the 16S rRNA identification suggested that isolate ABP5.2 is similar to Pseudomonas …

Flavonol Glucosylation: A Structural Investigation Of The Flavonol Specific 3-O Glucosyltransferase Cp3gt, Aaron S. Birchfield

Electronic Theses and Dissertations

Flavonoid glycosyltransferases (GTs), enzymes integral to plant ecological responses and human pharmacology, necessitate rigorous structural elucidation to decipher their mechanistic function and substrate specificity, particularly given their role in the biotransformation of diverse pharmacological agents and natural products. This investigation delved into a comprehensive exploration of the flavonol 3-O GT from Citrus paradisi (Cp3GT), scrutinizing the impact of a c-terminal c-myc/6x histidine tag on its enzymatic activity and substrate specificity, and successfully achieving its purification to apparent homogeneity. This established a strong foundation for potential future crystallographic and other structure/function analyses. Through the strategic implementation of site-directed mutagenesis, a thrombin …

Characterization Of Radiotolerance In Potato And Development Of A Gamma Radiation Phytosensor., Robert Graham Sears

Doctoral Dissertations

As humans pursue space travel and nuclear energy, the risk of harm from ionizing radiation increases. On Earth or in space, plants are essential to our personal and environmental health. Plants serve as sentinels, bioremediators and food sources in areas of high ionizing radiation, therefore it is essential to understand how ionizing radiation affects plant biology. This work aimed to understand plant responses to ionizing radiation in the potato chassis and apply that knowledge to generate novel phenotypes for nuclear energy and space applications. The first gamma radiation phytosensor was developed for monitoring at standoff distances greater than three meters. …