Food Science Commons^™

Comparative Analysis Of Amino Acid And Bioactive Peptide Profiles During Time-Resolved Fermentation Of Wheat And Stout Brewer's Spent Grain By Lactiplantibacillus Plantarum, Minh Quoc Ha, Angéla Juhász, Teguh Santoso, Mitchell G. Nye-Wood, Mathilde Berthevas, Zhiyu Zhao, Eileen Kitundu, Tony Chen, Michelle L. Colgrave, Thao T. Le

Research outputs 2022 to 2026

Brewers’ spent grain (BSG), a protein- and fibre-rich brewing by-product, contains bioactive compounds, yet techniques to fully recover and utilise these bioactive fractions remain underdeveloped. This study investigated the proteolytic transformation of two BSG types, wheat and stout BSG, during 10 days of fermentation with Lactiplantibacillus plantarum . Discovery and targeted LC-MS/MS proteomics were used to characterise the proteome, peptide release, and free amino acid profiles of BSG throughout fermentation. Proteomic analysis identified 503 proteins in wheat BSG and 340 in stout BSG. Wheat BSG generated higher peptide diversity than stout BSG (1,227 vs. 436 peptides, respectively), with peptide release …

From Proteome To Palate: Proteomics-Led Insights Into Sensory Quality In Oats (Avena Sativa L.), Clare E. O'Lone, Angéla Juhász, Utpal Bose, Haelee Fenton, Dini Ganesalingam, Allan Rattey, Michelle L. Colgrave

Research outputs 2022 to 2026

Background: Oats (Avena sativa L.) are a rapidly expanding ingredient in plant-based beverages, snacks and fermented products, where texture, flavour, aroma and colour shape consumer acceptance. A comparatively high lipid content (2–10% w/w) reduces milling efficiency and predisposes products to oxidative rancidity, while textural variation, bitterness and colour instability further constrain product development. The molecular basis for these processing and sensory challenges lies in the globulin-dominant grain proteome.

Scope and approach: This review synthesises advances in liquid chromatography–tandem mass spectrometry (LC-MS/MS) proteomics to quantitatively profile the four protein groups shaping oat sensory quality: storage, grain softness-related, lipid-associated, and metabolic and …

Physicochemical, Antioxidant, And Antimicrobial Properties Of Pasteurized Buffalo Milk Fortified With Roselle (Hibiscus Sabdariffa L.) Extract, Rikes Taruk Allo, Irma Isnafia Arief, Cahyo Budiman, Dicky Tri Utama, Irfan Dwidya Prijambada, Muhammad Saifur Rohman

Jurnal Ilmu Ternak Universitas Padjadjaran

This study aimed to evaluate the effects of roselle (Hibiscus sabdariffa L.) extract fortification on the physicochemical properties, nutritional composition, and functional activities of pasteurized buffalo milk. The study employed a 2 × 2 factorial Completely Randomized Design (CRD) with three technical replicates. The treatment factors were buffalo milk type (River and Toraja Belang) and roselle extract concentration (0% and 5%, v/v). Fortification with 5% roselle extract decreased the pH to 5.60 ± 0.07 and 5.51 ± 0.06 and the viscosity to 2.76 ± 0.75 and 2.42 ± 0.41 mPa·s, respectively, but did not affect water activity. River buffalo …

Assessment Of Pletekan Leaf Extract (Ruellia Tuberosa L.) As An Antibacterial Feed Additive For Broiler Chickens (Gallus Gallus Domesticus L.) Infected With Salmonella Sp., Nuria Istiqomah, Poncojari Wahyono, Rury Eryna Putri, Abdulkadir Rahardjanto

Jurnal Ilmu Ternak Universitas Padjadjaran

The use of synthetic antibiotics as antibiotic growth promoters (AGPs) in broiler chickens raises concerns over antibiotic residues and bacterial resistance, necessitating safer natural alternatives. Ruellia tuberosa L. contains various secondary metabolites with potential antibacterial properties; however, its in vivo efficacy against Salmonella sp. remains poorly documented. This study aimed to analyze the phytochemical composition of R. tuberosa leaf extract and evaluate its antibacterial activity against Salmonella sp. in broiler chickens. A Completely Randomized Design (CRD) was employed with eight treatments: a positive control (tetracycline), a negative control (Tween-80), and six extract concentrations (125, 250, 375, 500, 625, and 1000 …

Comparative Case Study Of Production Efficiency, Financial Performance, And Business Risk In Laying Hen Farms With Different Housing Systems And Management Conditions, Aliffa Mahayu Swastiratu, Fanny Widadie, Darsono

Jurnal Ilmu Ternak Universitas Padjadjaran

The laying hen sector continues to expand in response to increasing demand for animal protein; however, productivity remains sensitive to environmental conditions, particularly temperature fluctuations that affect production stability. The housing system represents a key management factor influencing farm performance. This study aims to compare production efficiency, financial performance, and business risk between closed-house and open-house laying hen systems. A comparative case study approach was applied to two commercial farms in Bayat District, Klaten Regency, representing each housing system. Data were analyzed using feed conversion ratio (FCR) and mortality rate for production efficiency, cost–revenue analysis, R/C ratio, and return on …

Advances In The Detection Of Ethyl Carbamate In Fermented Foods, Wang Yue, Zhao Yongpeng, Wang Siqi, Zhang Suyi, Ao Zonghua, Liu Shuliang, Li Jianlong

Food and Machinery

Ethyl carbamate, a group 2A carcinogen, has been widely detected in fermented foods and its safety has drawn increasing attention. Many countries have established limits for ethyl carbamate in fermented foods. This article reviews the contamination situation of ethyl carbamate, systematically summarizes and compares the detection methods of ethyl carbamate reported in both domestic and international literature in recent years, including chromatography, spectroscopy, electrochemical sensor method, paper strip colorimetry, and immunoassay. Finally, this article proposes the future development directions of residual detection technology for ethyl carbamate in fermented foods.

Research Progress On Protein Engineering Of Α-Amylase And Its Major Applications, Feng Yong, Ren Zhengwei

Food and Machinery

Alpha -Amylase is a glycoside hydrolase that efficiently catalyzes the hydrolysis of starch and is widely applied in various industrial fields such as food, textile, and paper industries. It plays a pivotal role particularly in food processing processes such as starch liquefaction, baking improvement, and juice clarification. However, natural α-amylases generally exhibit poor thermal stability, weak acid tolerance, and strong calcium ion dependency, which severely restrict their application in complex industrial environments. In recent years, protein engineering technologies, including directed evolution, rational design, and semi -rational design, have been extensively employed to the structural optimization and performance improvement of α-amylases. …

A Review Of Near-Infrared Spectroscopy For Pesticide Residue Detection In Fruits And Vegetables, Mai Xinyun, Hu Fangyang, He Lingling, Niu Junle, Wei Lanjie

Food and Machinery

Near -infrared spectroscopy (NIRS ) is valuable in pesticide residue detection for fruits and vegetables due to its nondestructive, rapid, and efficient nature. This review summarizes current research status in this field, compares the applicability of different spectral bands and imaging techniques, and analyzes challenges in single and mixed pesticide residue detection alongside enhancement strategies. Additionally, approaches to spectral preprocessing and feature wavelength selection are examined, with performance comparisons between chemometrics and deep learning models. Furthermore, the role of calibration transfer and transfer learning is discussed in improving model generalization across instruments and crop varieties. With continuous advancements in spectral …

Preparation Of Gleditsia Sinensis Polysaccharide Microemulsion And Their Hydrophobic Interactions With Quercetin, Xu Chenrui, Yao Qiuping, Deng Shenghui

Food and Machinery

[Objective] To investigate the formulation process of Gleditsia sinensis polysaccharide microemulsion and the hydrophobic interactions between the microemulsion and quercetin. [Methods] A pseudo -ternary phase diagram was drawn through compatibility experiments to optimize the formulation of the G.sinensis polysaccharide microemulsion. Based on the pyrene fluorescence probe technique, the effect of quercetin concentration on the I 1/I3 value in the microemulsion was examined to explore the hydrophobic and polar interactions between quercetin and the carrier in the G.sinensis polysaccharide microemulsion system. [Results] The prepared G.sinensis polysaccharide microemulsion was of the oil -in-water (O/W type ). The optimal formulation consisted of 35.4% …

Screening And Identification Of A Vibrio Parahaemolyticus -Antagonistic Fusion Weissella Confusa Y10-1 And Evaluation Of Its Bacteriostatic Activity, Yuan Baolong, Yi Han, Shen Naikun, Liang Ying, Tang Jiahao, Zhang Hongyan, Zhang Bin

Food and Machinery

[Objective] To isolate and screen lactic acid bacteria from the intestinal tract of marine fish with inhibitory activity against Vibrio parahaemolyticus and to investigate their antibacterial activity. [Methods] Using V.parahaemolyticus as the indicator bacterium, preliminary antibacterial screening was performed. The strain was identified through morphological, physiological, and biochemical characteristics as well as 16S rRNA gene sequence analysis. The antibacterial spectrum of the strain was determined, and the antibacterial stability, antibacterial mechanisms, and minimum inhibitory concentration (MIC ) of its cell -free supernatant (CFS ) were analyzed. [Results] A strain, Y10-1, showing strong antagonistic activity against V.parahaemolyticus was screened and identified …

Effects Of Microwave Decocting On Release Law Of Flavor Substances In Cod Soup, Zhang Chaomin, Li Xinxi, Feng Jianhui, Xu Yongxia

Food and Machinery

[Objective] To prepare cod soup using cod as the main raw material by microwave heating, with traditional boiling as the control, and to compare and analyze the effects of microwave decocting on the release law of flavor substances in cod soup. [Methods] Cod soup was processed at different durations (15,30,45, and 60 min ), followed by sensory evaluation. The changes in the contents of trichloroacetic acid (TCA )-soluble peptides, amino acid nitrogen, flavor nucleotides, organic acids, inorganic salt ions, and free amino acids in the soup were analyzed. [Results] The contents of TCA -soluble peptides, amino acid nitrogen, flavor nucleotides, …

Differential Analysis Of Volatile Components Between The Flesh And Peel Of Poria Cocos Based On Dynamic Headspace-Gc-Ms, Meng Xue, Xu Hui, Li Huixin, Cheng Chuanyun

Food and Machinery

[Objective] To compare the differences in volatile components between Poria cocos flesh and peel using dynamic headspace -gas chromatography -mass spectrometry (GC-MS). [Methods] Fresh P.cocos was used as the test sample. Volatile components were enriched by dynamic headspace adsorption, and the differences in volatile components between the flesh and peel were analyzed by GC -MS. Principal component analysis and cluster analysis were used to identify the main active components responsible for these differences. [Results] A total of 110 volatile compounds were detected from the peel and flesh of P.cocos, including 27 unique components in the flesh and 28 unique components …

Preparation And Certification Of Matrix Reference Material For Zearalenone In Whole Wheat Flour, Zhang Bing, Ye Jin, Li Peng, Wu Yu, Li Li, Dong Zemin, Wan Wengen, Wang Songxue

Food and Machinery

[Objective] To expand the varieties of matrix reference materials, improve the concentration levels of quantitative values, and meet the needs of market detection and scientific research, a matrix reference material for the compositional analysis of zearalenone (ZEN ) in whole wheat flour was developed. [Methods] Naturally contaminated wheat was used as the raw material, and the matrix reference material was prepared through cleaning, milling, and homogenization processes. It was vacuum -packed in aluminum foil pouches and sterilized by irradiation. The effects of grinding particle size and mixing time on the homogeneity of ZEN were investigated. Value assignment was conducted using …

Measurement Uncertainty Assessment For Total Aerobic Bacterial Count And Coliforms In Cooked Foods According To Rb/T 228, Liu Weiping, Wan Jianchun, Han Ying, Jiang Pengfei, Li Shixiang

Food and Machinery

[Objective] To evaluate the measurement uncertainty in the determination of total plate count and coliform bacteria in cooked foods, thereby improving the accuracy of test results. [Methods] The plate counting methods specified in GB 4789.2—2022 and GB 4789.3—2025 were employed to detect 18 cooked food samples under two conditions (A and B ). The combined standard uncertainty (uc(y)) and expanded uncertainty (U=2×uc(y)) were calculated in accordance with RB/T 228—2023. [Results] Based on intralaboratory reproducibility, the uc(y) for total plate count determination was 0.030 3 (U=0.060 6), and that for coliform bacteria determination was 0.179 7 (U=0.359 7). When all uncertainty …

Magnetic Surface Molecularly Imprinted Polymers For The Extraction And Analysis Of Tetracycline Residues From Fish Meat, Ye Run, Zhao Lei, Li Yuanxin, Cai Jing, Sun Wei, Chen Qiong, Zhang Wenqian

Food and Machinery

[Objective] This study aims to prepare magnetic surface molecularly imprinted polymers (MMIPs ) via a sol -gel method using tetracycline (TC) as the template molecule, and to combine them with spectrophotometry for the enrichment and detection of TC residues in fish meat. [Methods] Activated carbon (LAC ) is prepared from Magnolia grandiflora seeds using 60% (w/w) phosphoric acid solution as the activator. Magnetic activated carbon (MLAC ) is then obtained by co -precipitation -based magnetization of LAC. Using MLAC as the carrier and TC as the template molecule, MMIPs are synthesized, and their adsorption performance toward TC is investigated. The …

Geographical Origin Tracing Of Soybeans Using Electronic Tongue And Electronic Eye Combined With Multi-Level Information Fusion Based On Informer-Swin Transformer, Li Xin, Wang Zhiqiang, Han Tianrui, Xu Suchao

Food and Machinery

[Objective] To achieve rapid traceability detection of soybeans from different geographical origins. [Methods] A novel method for rapid soybean origin traceability is proposed based on the fusion of information from electronic tongue and electronic eye. The electronic tongue and electronic eye are used to collect taste fingerprint and visual image information, respectively, from soybeans of different origins. To address the differences between the temporal sequence features of electronic tongue signals and the spatial image features of electronic eye data, Informer and Swin Transformer are respectively employed to extract key features from the two types of data. A dual -modal feature …

Rapid Detection Of Tomato Nutritional Components Based On Nirs And Pso-Lightgbm, Shi Lichun, Wang Songwei, Wang Zhizhong

Food and Machinery

[Objective] To achieve rapid, non -destructive, and high -precision detection of six nutritional components in tomatoes, including dry matter, soluble sugar, total acid, vitamin C, soluble solids, and lycopene. [Methods] A tomato nutritional component detection model based on near -infrared spectroscopy (NIRS ) combined with light gradient boosting machine (LightGBM ) optimized using the particle swarm optimization (PSO ) algorithm (PSO -LightGBM ) was proposed. Using the six nutritional components as detection indicators, near -infrared spectral data (scanning range:400~2 500 nm) were collected from 471 tomato samples. Seven preprocessing methods were compared, and the optimal combination of standard normal variate …

A Non-Destructive Appearance Quality Detection Method For Primary-Processed Crayfish Based On Improved Yolov 11, Wang Xujia, Wang Shuqing, Hu Haoyu

Food and Machinery

[Objective] To achieve rapid and non -destructive online detection of the appearance quality of crayfish during primary processing and to improve detection accuracy and real -time performance in complex scenarios. [Methods] A dataset for crayfish appearance quality is constructed for complex scenarios on primary -processing production lines, and a crayfish appearance quality detection algorithm YOLO -CQ based on YOLOv 11n is proposed. DualConv is introduced to improve the C 3k2 module, enhancing the model's feature extraction capability while optimizing the allocation of computational resources. Deformable attention (DAT ) is integrated into the C 2PSA module, enabling the model to adaptively …

Design And Experimental Study On Drying Performance Of A Belt-Type Gas Jet Impingement Dryer, Liu Xinhan, Ju Haoyu, Zhang Weipeng, Geng Yahui, Miao Huan, Hou Liyuan

Food and Machinery

[Objective] To address the issues of uneven product quality, limited loading capacity, and the inability to achieve continuous drying in existing gas jet impingement dryers, a novel belt -type gas jet impingement dryer is designed to enhance the practicality and drying performance of the equipment. [Methods] The belt -type gas jet impingement dryer mainly consists of an airflow distribution chamber, a drying chamber, a centrifugal fan, a temperature control system, and a transmission system. The conveyor belt speed, airflow temperature, and air velocity can be adjusted according to the different materials. Simulations are conducted using Fluent software for airflow distribution …

Effects And Mechanisms Of Electrostatic Field-Assisted Temperature Rising On Inhibiting Color Browning Of Modified Atmosphere-Packaged Lamb Ribs During 10 ℃ Storage, Huang Zhiji, Wang Rong, Chen Bingyan, Zhu Lewei, Li Xinwei, Lin Yiqin, Lin Lijuan, Liu Yun, Lin Xiaozi, Li Weixin

Food and Machinery

[Objective] To address the problem of browning and oxidation in meat products processed from frozen lamb during shelf life. [Methods] Frozen sliced lamb ribs during temperature elevation were treated with an electrostatic field and then packaged using modified atmosphere packaging with different gas compositions. The samples were stored at 10 ℃ after packaging, and meat color, total viable count, oxidation level, and antioxidant enzyme activity were measured at 0,48,72, and 96 h of storage. [Results] After treatment with a 25 kV electrostatic field for 30 min, samples packaged under high -oxygen conditions (70% O2+30% CO2, EO group ) showed significantly …