Biotechnology Commons^™

Cgrp Modulation Of Intracellular Calcium Levels In Trigeminal Ganglion Neurons And Glia, Nicole M. Nalley

Graduate Theses/Dissertations

Elevated levels of calcitonin gene-related peptide (CGRP) are implicated in migraine and TMD pathology, but its effects on the excitability state of Aδ and C-fiber neurons and glia have not been investigated. The goal of this study was to determine changes mediated by CGRP on intracellular calcium levels in trigeminal ganglion neurons and glia in response to depolarizing stimulation. Intracellular calcium levels were determined in Aδ and C-fiber neurons of primary trigeminal ganglion cultures obtained from neonatal Sprague Dawley rats using Fura-2 and fluorescent microscopy. Cells were left untreated or preincubated for 2 hours with CGRP, then incubated with the …

Optimization Of L-Citrulline Production In Corynebacterium Glutamicum, And The Effect Of The Human Small Intestine Environment, Simran S. Ghulldu

Honors Undergraduate Theses

L-citrulline is a crucial amino acid involved in nitric oxide production, which supports cardiovascular health, physical performance, and intestinal well-being. Nitric oxide deficiency is linked to conditions like hypertension, highlighting the need for efficient L-citrulline production. This study attempts to enhance L-citrulline synthesis in Corynebacterium glutamicum, a bacterium widely used in industrial amino acid production, as well as test a The hypothesis is that overexpressing carAB, which encodes carbamoyl phosphate synthetase, will increase carbamoyl phosphate levels and boost L-citrulline production. Two C. glutamicum strains were used: a wild type and the CRBG strain, which has mutations that promote …

Investigating The Role Of Prostasin In Angiogenesis: A Chorioallantoic Membrane Model Approach, Zaima Aline

Honors Undergraduate Theses

Prostasin, also known as PRSS8 or CAP1, is a glycosylphosphatidylinositol (GPI)-anchored extracellular serine protease involved in various physiological and pathophysiological processes. Prostasin expression is observed during key phases of trophoblast invasion and placental development, critical steps in embryo implantation in Rhesus monkeys. This suggests a role for prostasin in extracellular matrix remodeling and tissue invasion, both essential for a healthy pregnancy. Moreover, prostasin appears vital for development as studies in mice show that a germline knockout of the gene leads to significant placental defects and eventual embryonic death, highlighting its importance in vascular and structural development within the placenta.

Building …

Unravelling The Role Of Two-Component Systems In Mycobacterium Abscessus, Andrea C. Molero Perez

Honors Undergraduate Theses

Mycobacterium abscessus (Mab) is an emerging pathogen within the nontuberculous bacteria (NTM) which poses a threat due to its complex multi-drug resistance and opportunistic nature. It is predicted that only 45.6% of patients will have successful treatment, with immunocompromised and cystic fibrosis patients being the most threatened. Moreover, investigating new treatment approaches is key to responding to this public health issue. Two-component systems (TCS) are regulatory pathways that play a pivotal role in bacterial survival and virulence. These systems are composed of a sensor histidine kinase (HK) and cognate response regulator (RR) protein that senses environmental cues and triggers specific …

Impact Of Color, Shape, And Typeface On Visual Attention: An Eye Tracking Study On Brand Logo, Suzayana Rosidah, Fransiskus Xaverius Ivan, Suatmi Murnani, Hafzatin Nurlatifa, Kristian Adi Nugraha, Sunu Wibirama

ASEAN Journal on Science and Technology for Development

In numerous cases, companies undertake logo redesigns to enhance brand perception. However, little attention has been paid to the impact of each element of the redesigned logo on visual attention and brand perception. To address this research gap, we collected data from eye tracking and self-report questionnaires of 30 participants during exposure to the old and new logos of a prominent bookstore in Indonesia. The results of the questionnaires revealed a significant relationship between the responses concerning color, shape, typeface, and those pertaining to visual attention (p < 0.05). Most participants were able to grasp the value of creativity, flexibility, progress, change, and strength in the new logo shape. The results of eye tracking show that color was the most influential factor that attracted visual attention in old (F(1.5, 43.4) = 14.905, p < 0.05) and new logos (F(1.7, 50) = 34.757, p < 0.05). This study suggests that companies should selectively choose a color scheme of a logo that better attracts the attention of consumers. In addition, our finding is promising as a practical guide for similar research, as well as a case study on how logo redesign affects brand perception and visual attention.

Engineering Geological And Groundwater Mapping Of Negara Brunei Darussalam For Urban Planning, Nur Thaqifah Junaidi, Siti Lieyana Azffri, Afiq Aiman Jaya, Muhammad Asri Akmal Suhip, Stefan Herwig Gödeke

ASEAN Journal on Science and Technology for Development

Engineering geology and Hydrogeology play a crucial role in the development of urban areas. Without a thorough geological and hydrogeological understanding, urban development can be risky and prone to numerous challenges. In this study, we aim to conduct engineering geological and groundwater mapping of Brunei utilizing data from 614 sacrificial investigative boreholes drilled over the course of 40 years for geotechnical investigation and housing development. Borehole parameters such as soil data, SPT N-values, depth to groundwater and surface elevation were gathered and evaluated. Soil investigation in the study area revealed predominantly clayey sediments with SPT N-values ranging from 0 to …

Regulation Of Haemodynamic Variables By Laguerre Polynomials Based Model Predictive Control., Sai Sandeep Boda, Hiren Kumar G. Patel, Khyati D. Mistry

ASEAN Journal on Science and Technology for Development

Purpose: Cardiac output (CO) and Mean Arterial Pressure (MAP) are two haemodynamic variables that need regulation in clinical situations, particularly in post-cardiac surgery patients. As it is difficult for clinical personnel to monitor continuously, automatic drug infusion to regulate MAP and CO is recommended and essential in the medical environment. Methods: Two input and two output patient model was used in this work to study the variation of haemodynamic variables in response to the infusion of Dopamine (DP) and Sodium Nitroprusside (SNP) drugs. Model predictive control (MPC) algorithm was exercised here as it was a well-established technique to regulate output …

From Theory To Practice: Simulating Zero Trust Architecture With Python For Cybersecurity, Pankaj R. Chandre, Bhagyashree D. Shendkar, Sulochana Sagar Madachane, Nikita Kulkarni, Sayalee Deshmukh

ASEAN Journal on Science and Technology for Development

The increasing complexity and frequency of cyber threats have necessitated the adoption of advanced security models, such as Zero Trust Architecture (ZTA). ZTA runs on the tenet that no entity whether inside or outside the network should be trusted by default, in contrast to conventional perimeter-based security solutions. This paper describes a thorough method for simulating and implementing Zero Trust Architecture in Python. We examine the essential elements and procedures of ZTA, such as identity verification, access control, and continuous monitoring, through a thorough analysis and step-by-step manual. The simulation shows how ZTA works to ensure strong network security and …

Neuro-Symbolic Ai: A Future Of Tomorrow, Pankaj Chandre, Parikshit Mahalle, Gitanjali Shinde, Bhagyashree Shendkar, Shraddha Kashid

ASEAN Journal on Science and Technology for Development

Neuro-Symbolic AI: A Future of Tomorrow" explores the convergence of neural learning and symbolic reasoning to advance artificial intelligence (AI) systems. Symbolic reasoning makes use of knowledge representation techniques and rule-based systems, whereas neural learning analyzes data using deep learning models. AI becomes more adept at fusing data-driven insights with deductive reasoning when these methods are integrated using hybrid models and differentiable reasoning techniques. Applications show enhanced diagnostic precision and decision-making skills in a variety of industries, including robotics, healthcare, and finance. To promote responsible AI development, regulatory frameworks and ethical principles address issues like bias and transparency. Future paths …

The Influence Of The Digital Economy On The Ecological Environment And Its Sustainable Development, Xinpan Lyu, Jianhua Dai

ASEAN Journal on Science and Technology for Development

This study aims to examine the impact of the digital economy on ecological sustainability and its development in China from 2000 to 2022. The study focuses on the role of innovation in the digital sector, where the adoption of big data technologies is considered, with reference to sustainable development, from an environmental perspective. A dynamic threshold model and, in particular, the Iterated Generalized Least Squares (IGLS) technique is used to analyze the given data.The research reveals a significant link between adopting digital technologies and managing environmental sustainability efficiently. To investigate the interconnection between the digital economy and ecological goals, it …

Exploring Dynamics And Effective Strategies For Tidal Flood Risk Reduction In Indonesia's Coastal Cities, Satria Yudha Adhitama, Diana Puspitasari, Lucia Sandra Budiman, Azis Musthofa

ASEAN Journal on Science and Technology for Development

Indonesia’s coastal regions have distinct charateristics. However, nearly all of these coastal regions are vulnerable to tidal floods. The purpose of this study is to determine the features of coastal regions in Indonesia that have been damaged by tidal floods, as well as the actions implemented to mitigate disaster risk. Thus study takes a qualitiative method with explanatory analysis. The Island Spatial Planning is used to classify Indonesia’s archipelagic regions.An urban area vulnerable to tidal flood was selected from each archipelagic region. The characteristics of coastal regions affected by tidal flood were identified by the tidal flood characteristics, coastal physiography. …

Analysis Of The Sustainability Management Of Coral Reef Ecosystem In The Marine Protected Area Of Wediombo, Gunungkidul, Nabilah L. Rohmah, Rika Harini, Sudrajat Sudrajat

ASEAN Journal on Science and Technology for Development

The Wediombo Coastal Area is a Marine Protected Area (MPA) with highly potential in coral reef ecosystems. According to the Millennium Ecosystem Assessment (MEA), coral reefs play a vital role in supporting human life by providing provisioning, supporting, regulating, and cultural services. However, the utilization of this area as a tourist destination and suboptimal management practices have led to the degradation of its coral reef ecosystems. Efective management strategies for the sustainability of coral reefs can have positive impacts across ecological, economic, social, technological, and institutional dimensions. Therefore, this study aims to analyze the sustainability management of coral reef ecosystem …

Wind Speed Forecasting: A Comparative Study Of Decomposition Techniques, Manisha Galphade, V.B. Nikam, Nilkamal More, Biplab Banerjee, Arvind W. Kiwelekar, Priyanka Sharma

ASEAN Journal on Science and Technology for Development

Renewable energy is sourced from natural resources that are continually available. Wind energy is a main category of renewable energy, which largely depends on wind speed. Accurate wind speed forecasting is essential for incorporating renewable energy into the electrical grid. Moreover, it is crucial to ensure the safety of wind turbines by anticipating extreme weather conditions and implementing necessary precautions. Predicting wind speed presents several challenges because of dynamic and complex nature of atmospheric conditions. Traditional methods for wind speed forecasting, such as statistical models and basic physical approaches, often face limitations in accuracy, flexibility, and handling of complex data …

Improving Ethanol Purity By Methanol Adsorption Using Mcm-41: A Study Of Kinetics And Thermodynamics For Industrial Applications, Ali A. Yahya, Nisreen S. Ali, Basma B. Hameed, Talib M. Albayati, Issam K. Salih, Riyadh S. Almukhtar, Narges Elmi Fard

ASEAN Journal on Science and Technology for Development

One of the by-products of the fermentation process that produces ethanol, which is mostly utilized in industry and food, is methanol. For methanol adsorption in batch operations, mixed amines modified (MCM-41) was utilized. A batch adsorption technique loaded with MCM-41 sorbent was used in the study to separate methanol from ethanol. This study used methanol at varying initial concentrations (40-80 mg/L). As a result, the effect of temperature, duration of adsorption, amount of adsorbent and initial concentration of pollutant in the field of ethanol alcohol purification using MCM-41 was investigated through the adsorption mechanism. In addition, first and pseudo-second order …

Kadysnet: A Novel Approach To Dyslexia Prediction In Children: Combining Handwritten Text Recognition With A Hybrid Cnn-Bilstm-Ctc Model And Personalized Learning Strategies, Shailesh Prabhakar Patil, Ravindra Sadashivrao Apare, Ravindra Honaji Borhade, Parikshit Narendra Mahalle

ASEAN Journal on Science and Technology for Development

This study introduces a novel approach for early dyslexia detection in children through automated handwriting analysis, integrating a hybrid CNN-BiLSTM-CTC architecture with personalized learning strategies. Our method combines a custom CNN-BiLSTM-CTC model with tailored educational interventions to support dyslexic learners. We analyzed children's handwritten text images in English, collected from specially designed tests involving word rewriting, sentence reconstruction, and paragraph composition, particularly challenging tasks for dyslexic individuals. Notably, our CNN-BiLSTM-CTC model achieved the best result with an accuracy of 97.67%, outperforming other architectures. Compared to pre-trained models like EfficientNetB7, DenseNet121, and MobileNetV2, our custom CNN-BiLSTM-CTC model demonstrated superior performance. Key …

Snake Optimization Algorithm For Fractional Order Controller In Three-Area Load Frequency Regulation, Shubra Goel, Omveer Singh

ASEAN Journal on Science and Technology for Development

The transition toward renewable energy-dominated power systems have accentuated the intricacies of frequency control, necessitating advanced regulatory mechanisms. This investigation articulates a tri-zonal frequency stabilization approach, employing a hybridized controller that synergizes Fuzzy Fractional-Order PI and Tilt-Integral-Derivative methodologies. The uniqueness of this approach is further amplified by the deployment of the Snake Optimization algorithm for precise parameter tuning. Set within a conventional grid topology integrated with assorted renewable energy sources, the study evaluates the controller’s adaptability and resilience through a series of comprehensive scenario-based analyses.

Leveraging Strategic Information Systems For Competitive Advantage: A Case Study Of Veolia As A Multinational Enterprise, Sohail Nawaz Sabir

ASEAN Journal on Science and Technology for Development

Instead of examining several components that link planning and strategy implementation, researchers have focused on the alignment of IT and business, as well as the Strategic Information Systems Planning (SISP) process. The goal of this study is to examine several approaches that can close the gap between IT project planning and execution. Therefore, the primary objective of the proposed study is to examine SISP implementation strategies that can be theorized in the context of Veolia, a global multinational corporation (MNC). The primary aspects influencing the SISP for this study are the frequency of planning, the function of IT/business managers, assessment …

Numerical And Experimental Investigation Of Mechanical Performance, Interface Pressure Distribution, And Structural Safety Of Glass Fiber-Reinforced Below-Knee Prosthetic Sockets, Saif M. Abbas, Kadhim K. Resan, Mohammed Ali Abdulrehman, Ali M. Flayyih

ASEAN Journal on Science and Technology for Development

In this study, below-knee prosthetic sockets reinforced with glass-fiber composites were investigated experimentally and numerically. In compliance with the ASTM guidelines, two lay-up configurations were constructed and tested for tensile and fatigue strengths. During the gait trials, the F-socket system was used to measure the interface pressure distribution. Stress, deformation, and safety factor responses under physiological loading conditions were simulated using complementary finite element analysis (FEA). With the yield strength, ultimate tensile strength, and fatigue life significantly improved in the reinforced group, the results demonstrated that the 10-layer laminate displayed superior mechanical performance relative to the 8-layer laminate; however, this …

Quality And Safety Analysis Of Integrated Bus Services And Feeder Transport In Surabaya, Indonesia, Desrina Yusi Irawati, Agrienta Bellanov, Armadeo Ruben Canariesa

ASEAN Journal on Science and Technology for Development

The Surabaya city government has developed public transportation through integrated bus services and feeder transport. These transportation systems include Trans Semanggi, Suroboyo Bus, and Wira Wiri Surabaya. The challenge facing these transportation programs is the low level of public participation in using public transportation. This research aims to provide a comprehensive investigation of the perceived service quality of integrated public transportation in Surabaya by combining the RESCA and SERVQUAL frameworks. The study contributes by highlighting service quality gaps across five customized dimensions and offering practical insights to improve user satisfaction and increase public transport usage. Each RESCA dimension incorporates RATER …

Editorial: A Decade Of Apasti – Advancing Regional Prosperity Through Science, Technology And Innovation, Basilios Tsikouras

ASEAN Journal on Science and Technology for Development

A decade after its establishment, APASTI has emerged as a key regional mechanism for promoting science, technology and innovation as drivers of sustainable development, resilience, and shared prosperity in Southeast Asia. This editorial highlights the contributions of APASTI to policy alignment, knowledge exchange, research visibility, and collaborative network-building across ASEAN, while also acknowledging ongoing constraints related to governance, capacity, funding disparities, and uneven regional integration. It introduces a special issue of 14 papers published in AJSTD that collectively evaluate the implementation and impact of APASTI across diverse sectors, including geohazards, energy, materials, health, infrastructure, transport, and information systems. By positioning …