Biotechnology Commons^™

Leukocyte Infiltration Profiles At The Site Of Intradermal Injection Of Silikon-1000 In Scleroderma-Prone Ucd200/206 And Healthy White Leghorn Chickens, Katee L. Johnson, Jossie M. Santamaria, Chrysta N. Beck, Gisela F. Erf

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Intradermal injections of silicone-based fillers for the face and body may cause chronic inflammation and fibrotic reactions in some individuals. Chickens from the UCD 200/206 White Leghorn (WL) line spontaneously develop a fibrotic autoimmune disease known as scleroderma/systemic sclerosis (SSc). Using the SSc-prone UCD and healthy WL chickens, a longitudinal study was conducted to examine the cellular inflammatory responses to intradermal (i.d.) injections of sterile SILIKON-1000. Specifically, the pulp (a skin derivative) of growing feathers (GFs) was i.d. injected with sterile SILIKON-1000 or phosphate-buffered saline (PBS) as injection control (8 and 3 birds/line, respectively; 10 µL/GF; 20 GFs/bird). Two GFs …

A Novel Silver-Ruthenium-Based Antimicrobial Kills Gram-Negative Bacteria Through Oxidative Stress-Induced Macromolecular Damage, Patrick O. Tawiah

Theses and Dissertations

The rise in drug-resistant bacteria, together with the decline in antibiotic development, requires new strategies for infectious disease control. Gram-negative pathogens are particularly challenging to combat due to their outer membrane. This study highlights the effectiveness of the silver-containing antimicrobial AGXX® against the Gram-negative bacterium Escherichia coli. AGXX® is a promising silver coating that presumably kills bacteria through the generation of reactive oxygen species (ROS). However, neither AGXX®’s mode of action is fully understood, nor have its effects on Gram-negative bacteria or bacterial response and defense mechanisms towards AGXX® been studied in detail. Here, I report that the bactericidal …

Minimizing Cpu Utilization For Job Scheduling Problems By The Advanced Round Robin Method: A Pragmatic Perspective, Haribhau R Bhapkar, Pankaj R Chandre, Parikshit Mahalle

ASEAN Journal on Science and Technology for Development

CPU scheduling issues include minimizing waiting time for processes, ensuring fairness in resource allocation, and optimizing throughput. Balancing these objectives can be challenging, as improving one aspect may negatively impact another, making it essential to design efficient scheduling algorithms. This work presents an innovative approach to enhance CPU utilization through the development of a Result-Based Round Robin Scheduling Algorithm. Traditional Round Robin Scheduling methods often face challenges in efficiently allocating CPU time, leading to suboptimal system performance. In response to these limitations, the proposed method introduces a result-oriented strategy that dynamically adjusts time quantum allocations based on the execution progress …

The Effect Of Blanching And Steaming Pretreatment On The Quality Of Durian (Durio Zibenthinus Murr.) Seed Flour, Nabilah Daniel, Rosnah Shamsudin, Mohd Sabri Pak Dek, Jhauharotul Muchlisyiyah, Masmunira Rambli

ASEAN Journal on Science and Technology for Development

Durian (Durio zibethinus Murr.), the "King of Fruits," has seeds that offer nutritional benefits, including high fiber for gut health and high protein for muscle growth. However, natural browning enzymes can limit its use in baking. This study investigates the comparative effects of blanching and steaming durations (5, 10, 15, 20, and 25 minutes) on the color and techno-functional properties of durian seed flour (DSF), providing an optimization-based approach rarely reported for this material.. Durian seeds were sourced from various stalls in Malaysia, washed, soaked in 0.6% sodium metabisulfite, and then blanched or steamed for for varying durations as …

Evaluation Of The Dnazyme Gr5 For The Determination Of Lead Speciation In Natural Waters, Gaganprit Gill

Theses and Dissertations (Comprehensive)

This thesis explores the development and application of a DNAzyme-based biosensor designed to detect labile metal species in environmental samples. Real time and on-site monitoring of labile metal fractions would make a valuable contribution to environmental management, as these fractions are the most bioavailable and pose significant toxicity risks to aquatic organisms. Conventional methods for detecting labile metals, while effective, are often burdened by high costs, complexity, and lengthy processing times, making them less ideal for rapid and widespread environmental assessments.

The first manuscript of this thesis (chapter 2) establishes the fundamental capabilities of the Pb²⁺-specific DNAzyme GR5 …

Engineering Geological And Groundwater Mapping Of Negara Brunei Darussalam For Urban Planning, Nur Thaqifah Junaidi, Siti Lieyana Azffri, Afiq Aiman Jaya, Muhammad Asri Akmal Suhip, Stefan Herwig Gödeke

ASEAN Journal on Science and Technology for Development

Engineering geology and Hydrogeology play a crucial role in the development of urban areas. Without a thorough geological and hydrogeological understanding, urban development can be risky and prone to numerous challenges. In this study, we aim to conduct engineering geological and groundwater mapping of Brunei utilizing data from 614 sacrificial investigative boreholes drilled over the course of 40 years for geotechnical investigation and housing development. Borehole parameters such as soil data, SPT N-values, depth to groundwater and surface elevation were gathered and evaluated. Soil investigation in the study area revealed predominantly clayey sediments with SPT N-values ranging from 0 to …

From Theory To Practice: Simulating Zero Trust Architecture With Python For Cybersecurity, Pankaj R. Chandre, Bhagyashree D. Shendkar, Sulochana Sagar Madachane, Nikita Kulkarni, Sayalee Deshmukh

ASEAN Journal on Science and Technology for Development

The increasing complexity and frequency of cyber threats have necessitated the adoption of advanced security models, such as Zero Trust Architecture (ZTA). ZTA runs on the tenet that no entity whether inside or outside the network should be trusted by default, in contrast to conventional perimeter-based security solutions. This paper describes a thorough method for simulating and implementing Zero Trust Architecture in Python. We examine the essential elements and procedures of ZTA, such as identity verification, access control, and continuous monitoring, through a thorough analysis and step-by-step manual. The simulation shows how ZTA works to ensure strong network security and …

Solid Waste Management Strategies In Coastal Areas Affected By Seawater Flood Disaster, Adib Khoirul Anas, Maryono Maryono, Hartuti Purnaweni

ASEAN Journal on Science and Technology for Development

Solid waste in areas affected by seawater floods must be handled specifically because people often dispose of solid waste on flooded land. This research aims to identify the performance of the community to manage solid waste and solid waste management strategy, solid waste measurement using SNI method 19-3964-1994. Analysis of land affected by seawater floods shows that 12 villages are flooded. Communities in coastal areas are not optimal in managing solid waste because the government has never given directions to manage solid waste in a good way, the facilities and marketing are inadequate so that solid waste is often not …

Kadysnet: A Novel Approach To Dyslexia Prediction In Children: Combining Handwritten Text Recognition With A Hybrid Cnn-Bilstm-Ctc Model And Personalized Learning Strategies, Shailesh Prabhakar Patil, Ravindra Sadashivrao Apare, Ravindra Honaji Borhade, Parikshit Narendra Mahalle

ASEAN Journal on Science and Technology for Development

This study introduces a novel approach for early dyslexia detection in children through automated handwriting analysis, integrating a hybrid CNN-BiLSTM-CTC architecture with personalized learning strategies. Our method combines a custom CNN-BiLSTM-CTC model with tailored educational interventions to support dyslexic learners. We analyzed children's handwritten text images in English, collected from specially designed tests involving word rewriting, sentence reconstruction, and paragraph composition, particularly challenging tasks for dyslexic individuals. Notably, our CNN-BiLSTM-CTC model achieved the best result with an accuracy of 97.67%, outperforming other architectures. Compared to pre-trained models like EfficientNetB7, DenseNet121, and MobileNetV2, our custom CNN-BiLSTM-CTC model demonstrated superior performance. Key …

Unwritten Rules And Oral Transmission In Architectural Proportion Systems: Case Study Of Bubungan Tinggi House, Huzairin. Muhammad Deddy, Dila Nadya Andini, Rudi Hartono, Anna Oktaviana, Dahliani Dahliani

ASEAN Journal on Science and Technology for Development

The proportional systems in vernacular architecture often exist without formal written codification, raising questions about their transmission across generations. Bubungan Tinggi House which is a replica of the Banjar Kingdom palace was built by Banjarese merchants and aristocrats from the 16th to the 19th centuries, showing a uniform aesthetic quality, which indicates a regularity in the proportions of its form. However, no historical written manuals detailing these proportions are known to exist. To prove the existence of regularity in the Bubungan Tinggi House proportion system, research was conducted by taking 7 Bubungan Tinggi House samples spread across South Kalimantan province, …

Leveraging Strategic Information Systems For Competitive Advantage: A Case Study Of Veolia As A Multinational Enterprise, Sohail Nawaz Sabir

ASEAN Journal on Science and Technology for Development

Instead of examining several components that link planning and strategy implementation, researchers have focused on the alignment of IT and business, as well as the Strategic Information Systems Planning (SISP) process. The goal of this study is to examine several approaches that can close the gap between IT project planning and execution. Therefore, the primary objective of the proposed study is to examine SISP implementation strategies that can be theorized in the context of Veolia, a global multinational corporation (MNC). The primary aspects influencing the SISP for this study are the frequency of planning, the function of IT/business managers, assessment …

Numerical And Experimental Investigation Of Mechanical Performance, Interface Pressure Distribution, And Structural Safety Of Glass Fiber-Reinforced Below-Knee Prosthetic Sockets, Saif M. Abbas, Kadhim K. Resan, Mohammed Ali Abdulrehman, Ali M. Flayyih

ASEAN Journal on Science and Technology for Development

In this study, below-knee prosthetic sockets reinforced with glass-fiber composites were investigated experimentally and numerically. In compliance with the ASTM guidelines, two lay-up configurations were constructed and tested for tensile and fatigue strengths. During the gait trials, the F-socket system was used to measure the interface pressure distribution. Stress, deformation, and safety factor responses under physiological loading conditions were simulated using complementary finite element analysis (FEA). With the yield strength, ultimate tensile strength, and fatigue life significantly improved in the reinforced group, the results demonstrated that the 10-layer laminate displayed superior mechanical performance relative to the 8-layer laminate; however, this …

Performance Evaluation And Economic Analysis Of Lithium Iron Phosphate (Lfp) And Nickel Manganese Cobalt (Nmc) Batteries In Electric Vehicles In Indonesia, Yasa Sanusi, Fatwa Tresna Radityan, Farrah Anis Fazliatul Adnan, Jong Soo Rhee, Dianta Ginting

ASEAN Journal on Science and Technology for Development

The transfer to electric vehicles (EVs) in more and more countries is a response to the increasing problems of climate change and air pollution. Electric cars solve this issue as they have zero emissions. EVs can significantly reduce carbon and air pollution, depending on the charging energy mix. In Indonesia, lithium ferro phosphate and nickel manganese cobalt are among the two most preferred types of batteries for EVs, and both have their advantages. This study evaluates the performance of these batteries in terms of power, range, charging speed, and thermal properties, using a range of battery capacities from 26.7 kWh …

Manure Handling In Circular Farming: An In Vitro Study Of Fermented Poultry Manure As Unconventional Feed For Ruminants, Dimas Hand Vidya Paradhipta, De Lavida Padma Terrakota, Ali Agus, Andriyani Astuti, Viagian Pastawan, Kharisma Taufiqa Hidayah

ASEAN Journal on Science and Technology for Development

The development of the poultry industry must consider the principle of circular economy. Therefore, this study aims to develop a method of fermenting poultry manure as a new, unconventional feed, which can support the model of circular farming. Poultry manure obtained from laying hens was mixed with cassava solid waste at a ratio of 7:3 to maintain proper moisture content. The mixed manure fermented for 14 days into a 30 kg silo in triplicate using different additives, namely Lactiplantibacillus plantarum FNCC 0020 at 1 x 10⁵ cfu/g (LP), Bacillus cereus LS2B as proteolytic bacteria at 1 x …

Numerical Modeling Of Performance And Phenomena In A Single Proton Exchange Membrane Fuel Cell, Doan Nguyen Cong, Hiep Nguyen Ha

ASEAN Journal on Science and Technology for Development

One of the methods to protect our environment is to convert the fuel's chemical energy directly into electricity, bypassing the fuel's combustion process. This method is implemented in devices called fuel cells. Among them, the proton exchange membrane fuel cell (PEMFC) is widely used in practice because it has outstanding advantages such as high power density and efficiency, low operating temperature and noise, compactness, fast start-up, and, most importantly, only releases water, entirely safe for the environment. The paper presents a method for selecting materials for manufacturing PEMFC components, making a single PEMFC model based on the materials chosen, then …

Neuro-Symbolic Ai: A Future Of Tomorrow, Pankaj Chandre, Parikshit Mahalle, Gitanjali Shinde, Bhagyashree Shendkar, Shraddha Kashid

ASEAN Journal on Science and Technology for Development

Neuro-Symbolic AI: A Future of Tomorrow" explores the convergence of neural learning and symbolic reasoning to advance artificial intelligence (AI) systems. Symbolic reasoning makes use of knowledge representation techniques and rule-based systems, whereas neural learning analyzes data using deep learning models. AI becomes more adept at fusing data-driven insights with deductive reasoning when these methods are integrated using hybrid models and differentiable reasoning techniques. Applications show enhanced diagnostic precision and decision-making skills in a variety of industries, including robotics, healthcare, and finance. To promote responsible AI development, regulatory frameworks and ethical principles address issues like bias and transparency. Future paths …

Analysis Of The Sustainability Management Of Coral Reef Ecosystem In The Marine Protected Area Of Wediombo, Gunungkidul, Nabilah L. Rohmah, Rika Harini, Sudrajat Sudrajat

ASEAN Journal on Science and Technology for Development

The Wediombo Coastal Area is a Marine Protected Area (MPA) with highly potential in coral reef ecosystems. According to the Millennium Ecosystem Assessment (MEA), coral reefs play a vital role in supporting human life by providing provisioning, supporting, regulating, and cultural services. However, the utilization of this area as a tourist destination and suboptimal management practices have led to the degradation of its coral reef ecosystems. Efective management strategies for the sustainability of coral reefs can have positive impacts across ecological, economic, social, technological, and institutional dimensions. Therefore, this study aims to analyze the sustainability management of coral reef ecosystem …

Potential Of Green Synthesis Of Silver And Gold Nanoparticles For The Management Of Metabolic Associated Steatotic Liver Disease: A Mini Review, Atiqah Suhaili, Ya Chee Lim, Mei Ann Lim, Lee Hoon Lim, Mohammad Mansoob Khan, Monowarul Mobin Siddique

ASEAN Journal on Science and Technology for Development

Metabolic-associated steatotic liver disease is a metabolic disorder with limited pharmacological treatment options and only one approved medication to date. MASLD can be asymptomatic in its earlier stage and hence, prevention of this disease progression is the most effective approach. Recently, green-synthesized nanoparticles, particularly silver (AgNPs) and gold nanoparticles (AuNPs), have gained interest due to their eco-friendly production, biocompatibility, and bioactive properties. This review highlights the biosynthesis of AgNPs and AuNPs using medicinal plant extracts and their reported biological effects relevant to MASLD management including anti-hyperlipidemic, anti-diabetic, antioxidant and anti-inflammatory activities. While findings are promising, further mechanistic studies and preclinical …

Quality And Safety Analysis Of Integrated Bus Services And Feeder Transport In Surabaya, Indonesia, Desrina Yusi Irawati, Agrienta Bellanov, Armadeo Ruben Canariesa

ASEAN Journal on Science and Technology for Development

The Surabaya city government has developed public transportation through integrated bus services and feeder transport. These transportation systems include Trans Semanggi, Suroboyo Bus, and Wira Wiri Surabaya. The challenge facing these transportation programs is the low level of public participation in using public transportation. This research aims to provide a comprehensive investigation of the perceived service quality of integrated public transportation in Surabaya by combining the RESCA and SERVQUAL frameworks. The study contributes by highlighting service quality gaps across five customized dimensions and offering practical insights to improve user satisfaction and increase public transport usage. Each RESCA dimension incorporates RATER …

Seismic Vulnerability Assessment With Rapid Visual Screening Of Hospital Buildings In Maguindanao Province, Philippines, Ebrahim B. Kayog, Kristine S. Companion, Kenny B. Cantila, Elizabeth Edan M. Albiento

ASEAN Journal on Science and Technology for Development

Hospitals are expected to maintain operations during and after earthquakes, yet many facilities in the Philippines remain structurally and non-structurally vulnerable. This study evaluates the seismic vulnerability of selected public hospitals in Maguindanao Province, located near the Cotabato Trench and the Western Mindanao Fault, using the FEMA P-154 (3rd ed.) Rapid Visual Screening (RVS) Level-1 procedure. Four government hospitals, comprising 53 buildings constructed between 1980 and 2025, were assessed through a systematic visual inspection and review of available secondary records. Basic structural hazard scores were adjusted using FEMA score modifiers (performance modification factors) to compute Final Level-1 scores (Sₗ₁) and …