Biotechnology Commons^™

Fta™ Card Use For Detection Of Mycoplasma Gallisepticum In Wild Birds, Linda Purvis, Dawn E.W. Drumtra

Georgia Journal of Science

Flinders Technology Associates (FTA) cards are increasingly accepted as an accurate method for nucleic acid detection, including testing for the presence of various avian diseases. However, the accuracy of FTA cards for detection of Mycoplasma gallisepticum (MG) in wild birds has not been published. Traditional techniques for testing MG in birds include serum plate agglutanation (SPA), hemagglutination inhibition (HI), polymerase chain reaction (PCR), and enzyme linked immunosorbent assay (ELISA) all of which are time consuming and require significant financial investment into specialized equipment. We tested the efficacy of using FTA cards to detect MG in wild bird carcasses collected in …

Characterization Of The Slc7a5/Slc3a2 Amino Acid Transporter In Human Pleural Mesothelial Cells, Ann Francis

Biotechnology Theses

The SLC7A5/SLC3A2 amino acid transporter moves essential amino acids, such as leucine, phenylalanine, and tryptophan, across the cellular plasma membrane. SLC7A5 overexpression was identified in diverse cancers, suggesting a role in tumorigenesis and cancer cell proliferation. However, the role of SLC7A5/SLC3A2 in other aggressive cellular phenotypes, including mesothelial mesenchymal transition (MesoMT), remains unexplored. RNA sequencing analyses identified SLC7A5 as one of three highly upregulated SLCs in mesothelial cell derived myofibroblasts. This sodium independent transporter exhibits a high affinity for branched chain amino acids such as leucine; however, what role, if any, this channel plays in MesoMT is unclear. SLC7A5 knockdown …

Genetic Property Rhetoric And The Public Domain, Dave Fagundes

Texas A&M Journal of Property Law

Among the many themes that illuminate Jorge Contreras’s The Genome Defense, this Essay focuses on property. It considers the rhetorical role played by property in convincing both courts and the public that patenting genetic data was legally and ethically objectionable. This Essay begins by locating property—and public property—as a major theme in the dialogue surrounding the Myriad litigation. It then situates this rhetorical move in the context of property-talk generally, highlighting the distinctiveness of leveraging public rather than private ownership as a means of accessing moral intuitions. Finally, this Essay reflects on what the use of public property rhetoric …

Functional Analysis Of Ligand Binding Domain 2 Of Outer Surface Protein C Of Borreliella Burgdorferi, Gavin Z. Chambers

Theses and Dissertations

Lyme disease, caused by the spirochete Borreliella burgdorferi, is the most common vector-borne infection in North America. A critical determinant of early mammalian infection is outer surface protein C (OspC), a highly variable lipoprotein that is indispensable for spirochete survival but whose precise function remains unresolved. OspC has been proposed to mediate diverse roles, including complement evasion, adhesion, tissue dissemination, and antiphagocytic activity, supporting the view that it is a multifunctional virulence factor. Structural studies have identified two putative ligand-binding domains (LBD1 and LBD2), with LBD2 forming a solvent-exposed crown at the membrane-distal end of the OspC dimer.

To …

Influence Of Unmanned Aerial Vehicle (Uav) Payloads And Flight Speeds On Pesticide Droplet Deposition: Indoor Testing Rig Study, Siti Amni Ismail, Azmi Yahya, Ahmad Suhaizi Mat Su, Anas Mohd Mustafah, Norhayu Asib

ASEAN Journal on Science and Technology for Development

This study aims to determine droplet deposition resulting from dynamic change in payloads and flight speeds of Unmanned Aerial Vehicle (UAV) during operation. The study was conducted at Universiti Putra Malaysia, Serdang, Malaysia. The Advansia A1 hexacopter was first set to fly at combinations of 3 payloads (3.3, 6.6, and 9.9 kg) with 3 flight speeds (3, 5 and 7 m/s) to collect individual rotor RPM, which will later be used to set the rotors’ speed during indoor testing experiment. During the experiment, actual pesticide (Alosa EC) was used at 0.5% concentration in the spray deposition test. The droplet deposition …

Every Publication Needs An Author Verified Tl;Dr Statement, Sarah Oerther, Daniel B. Oerther

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

No abstract provided.

การวิเคราะห์เมตาโบโลมิกส์ในน้ำนมเหลืองและน้ำนมจากแม่สุกรที่ได้รับอาหารเสริมซินไบโอติก, เมธาพร เจือจันทร์

Chulalongkorn University Theses and Dissertations (Chula ETD)

ปัจจุบันมีการใช้สารเสริมอาหารสัตว์ในกลุ่มจุลินทรีย์โพรไบโอติกและซินไบโอติกเพื่อทดแทนการใช้สารปฏิชีวนะและลดปัญหาการเกิดเชื้อดื้อยาในอุตสาหกรรมการผลิตสุกร ซึ่งสารเสริมชีวนะดังกล่าวส่งผลในเชิงการส่งเสริมสุขภาพของแม่สุกร เพิ่มประสิทธิภาพในการสังเคราะห์องค์ประกอบและสารภูมิคุ้มกันในน้ำนม รวมทั้งช่วยลดอัตราการเกิดโรคท้องเสียในลูกสุกร อย่างไรก็ตาม การศึกษาข้อมูลสารชีวโมเลกุลขนาดเล็กหรือสารเมตาบอไลต์ในน้ำนมจากแม่สุกรที่ได้รับอาหารเสริมซินไบโอติกยังมีอยู่อย่างจำกัด ดังนั้น งานวิจัยนี้จึงมีวัตถุประสงค์เพื่อวิเคราะห์และเปรียบเทียบข้อมูลสารชีวโมเลกุลในน้ำนมจากแม่สุกรที่ได้รับอาหารที่มีการเสริมซินไบโอติก โดยพิจารณาร่วมกับอิทธิพลของระยะการให้นมและอิทธิพลจากลำดับครอกที่แตกต่างกันของแม่สุกร ผลการวิจัยพบว่าสามารถระบุชนิดของสารเมตาบอไลต์และกรดไขมันในตัวอย่างน้ำนมสุกรได้ทั้งหมด 47 และ 23 สาร โดยใช้เทคนิค 1H-NMR และ GC-FID ตามลำดับ ผลการวิเคราะห์ทางสถิติพหุตัวแปรแสดงให้เห็นว่าอิทธิพลจากระยะการให้นมส่งผลต่อการเปลี่ยนแปลงข้อมูลสารชีวโมเลกุลในน้ำนมสุกรอย่างชัดเจน เมื่อพิจารณาตัวอย่างน้ำนมที่เก็บในระยะเดียวกัน พบว่าอิทธิพลของการได้รับอาหารเสริมซินไบโอติกส่งผลต่อความแตกต่างของข้อมูลสาร เมตาบอไลต์ในตัวอย่างน้ำนมเหลืองและน้ำนมในวันที่ 10 เท่านั้น แต่อิทธิพลดังกล่าวส่งผลต่อการเปลี่ยนแปลงข้อมูลกรดไขมันตลอดทุกช่วงระยะการให้นม โดยสามารถใช้การเปลี่ยนแปลงปริมาณสัมพัทธ์ของ adenine, hypoxanthine, taurine, linolenic acid, linoleic acid และ lauric acid เป็นตัวบ่งชี้ทางชีวภาพเพื่อระบุความแตกต่างของน้ำนมจากแม่สุกรที่ได้รับอาหารเสริมซินไบโอติกได้ ทั้งนี้อิทธิพลจากลำดับครอกของแม่สุกรไม่ส่งผลต่อความแตกต่างของข้อมูลสารเมตาบอไลต์ แต่ส่งผลต่อความแตกต่างของข้อมูลกรดไขมันอย่างชัดเจนตลอดทุกช่วงระยะการให้นม โดยเฉพาะในแม่สุกรกลุ่มที่ได้รับอาหารเสริมซินไบโอติก ผลการวิจัยนี้แสดงให้เห็นถึงการประยุกต์ใช้เทคโนโลยีเมตาโบโลมิกส์ในการศึกษาการเปลี่ยนแปลงข้อมูลสารชีวโมเลกุลในน้ำนมสุกร และระบุชนิดของตัวบ่งชี้ทางชีวภาพต่ออิทธิพลของการได้รับอาหารเสริมซินไบโอติกได้อย่างมีประสิทธิภาพ

A Novel Silver-Ruthenium-Based Antimicrobial Kills Gram-Negative Bacteria Through Oxidative Stress-Induced Macromolecular Damage, Patrick O. Tawiah

Theses and Dissertations

The rise in drug-resistant bacteria, together with the decline in antibiotic development, requires new strategies for infectious disease control. Gram-negative pathogens are particularly challenging to combat due to their outer membrane. This study highlights the effectiveness of the silver-containing antimicrobial AGXX® against the Gram-negative bacterium Escherichia coli. AGXX® is a promising silver coating that presumably kills bacteria through the generation of reactive oxygen species (ROS). However, neither AGXX®’s mode of action is fully understood, nor have its effects on Gram-negative bacteria or bacterial response and defense mechanisms towards AGXX® been studied in detail. Here, I report that the bactericidal …

Engineering Geological And Groundwater Mapping Of Negara Brunei Darussalam For Urban Planning, Nur Thaqifah Junaidi, Siti Lieyana Azffri, Afiq Aiman Jaya, Muhammad Asri Akmal Suhip, Stefan Herwig Gödeke

ASEAN Journal on Science and Technology for Development

Engineering geology and Hydrogeology play a crucial role in the development of urban areas. Without a thorough geological and hydrogeological understanding, urban development can be risky and prone to numerous challenges. In this study, we aim to conduct engineering geological and groundwater mapping of Brunei utilizing data from 614 sacrificial investigative boreholes drilled over the course of 40 years for geotechnical investigation and housing development. Borehole parameters such as soil data, SPT N-values, depth to groundwater and surface elevation were gathered and evaluated. Soil investigation in the study area revealed predominantly clayey sediments with SPT N-values ranging from 0 to …

Kadysnet: A Novel Approach To Dyslexia Prediction In Children: Combining Handwritten Text Recognition With A Hybrid Cnn-Bilstm-Ctc Model And Personalized Learning Strategies, Shailesh Prabhakar Patil, Ravindra Sadashivrao Apare, Ravindra Honaji Borhade, Parikshit Narendra Mahalle

ASEAN Journal on Science and Technology for Development

This study introduces a novel approach for early dyslexia detection in children through automated handwriting analysis, integrating a hybrid CNN-BiLSTM-CTC architecture with personalized learning strategies. Our method combines a custom CNN-BiLSTM-CTC model with tailored educational interventions to support dyslexic learners. We analyzed children's handwritten text images in English, collected from specially designed tests involving word rewriting, sentence reconstruction, and paragraph composition, particularly challenging tasks for dyslexic individuals. Notably, our CNN-BiLSTM-CTC model achieved the best result with an accuracy of 97.67%, outperforming other architectures. Compared to pre-trained models like EfficientNetB7, DenseNet121, and MobileNetV2, our custom CNN-BiLSTM-CTC model demonstrated superior performance. Key …

Unwritten Rules And Oral Transmission In Architectural Proportion Systems: Case Study Of Bubungan Tinggi House, Huzairin. Muhammad Deddy, Dila Nadya Andini, Rudi Hartono, Anna Oktaviana, Dahliani Dahliani

ASEAN Journal on Science and Technology for Development

The proportional systems in vernacular architecture often exist without formal written codification, raising questions about their transmission across generations. Bubungan Tinggi House which is a replica of the Banjar Kingdom palace was built by Banjarese merchants and aristocrats from the 16th to the 19th centuries, showing a uniform aesthetic quality, which indicates a regularity in the proportions of its form. However, no historical written manuals detailing these proportions are known to exist. To prove the existence of regularity in the Bubungan Tinggi House proportion system, research was conducted by taking 7 Bubungan Tinggi House samples spread across South Kalimantan province, …

Leveraging Strategic Information Systems For Competitive Advantage: A Case Study Of Veolia As A Multinational Enterprise, Sohail Nawaz Sabir

ASEAN Journal on Science and Technology for Development

Instead of examining several components that link planning and strategy implementation, researchers have focused on the alignment of IT and business, as well as the Strategic Information Systems Planning (SISP) process. The goal of this study is to examine several approaches that can close the gap between IT project planning and execution. Therefore, the primary objective of the proposed study is to examine SISP implementation strategies that can be theorized in the context of Veolia, a global multinational corporation (MNC). The primary aspects influencing the SISP for this study are the frequency of planning, the function of IT/business managers, assessment …

Numerical And Experimental Investigation Of Mechanical Performance, Interface Pressure Distribution, And Structural Safety Of Glass Fiber-Reinforced Below-Knee Prosthetic Sockets, Saif M. Abbas, Kadhim K. Resan, Mohammed Ali Abdulrehman, Ali M. Flayyih

ASEAN Journal on Science and Technology for Development

In this study, below-knee prosthetic sockets reinforced with glass-fiber composites were investigated experimentally and numerically. In compliance with the ASTM guidelines, two lay-up configurations were constructed and tested for tensile and fatigue strengths. During the gait trials, the F-socket system was used to measure the interface pressure distribution. Stress, deformation, and safety factor responses under physiological loading conditions were simulated using complementary finite element analysis (FEA). With the yield strength, ultimate tensile strength, and fatigue life significantly improved in the reinforced group, the results demonstrated that the 10-layer laminate displayed superior mechanical performance relative to the 8-layer laminate; however, this …

Performance Evaluation And Economic Analysis Of Lithium Iron Phosphate (Lfp) And Nickel Manganese Cobalt (Nmc) Batteries In Electric Vehicles In Indonesia, Yasa Sanusi, Fatwa Tresna Radityan, Farrah Anis Fazliatul Adnan, Jong Soo Rhee, Dianta Ginting

ASEAN Journal on Science and Technology for Development

The transfer to electric vehicles (EVs) in more and more countries is a response to the increasing problems of climate change and air pollution. Electric cars solve this issue as they have zero emissions. EVs can significantly reduce carbon and air pollution, depending on the charging energy mix. In Indonesia, lithium ferro phosphate and nickel manganese cobalt are among the two most preferred types of batteries for EVs, and both have their advantages. This study evaluates the performance of these batteries in terms of power, range, charging speed, and thermal properties, using a range of battery capacities from 26.7 kWh …

Analysis Of The Sustainability Management Of Coral Reef Ecosystem In The Marine Protected Area Of Wediombo, Gunungkidul, Nabilah L. Rohmah, Rika Harini, Sudrajat Sudrajat

ASEAN Journal on Science and Technology for Development

The Wediombo Coastal Area is a Marine Protected Area (MPA) with highly potential in coral reef ecosystems. According to the Millennium Ecosystem Assessment (MEA), coral reefs play a vital role in supporting human life by providing provisioning, supporting, regulating, and cultural services. However, the utilization of this area as a tourist destination and suboptimal management practices have led to the degradation of its coral reef ecosystems. Efective management strategies for the sustainability of coral reefs can have positive impacts across ecological, economic, social, technological, and institutional dimensions. Therefore, this study aims to analyze the sustainability management of coral reef ecosystem …

Potential Of Green Synthesis Of Silver And Gold Nanoparticles For The Management Of Metabolic Associated Steatotic Liver Disease: A Mini Review, Atiqah Suhaili, Ya Chee Lim, Mei Ann Lim, Lee Hoon Lim, Mohammad Mansoob Khan, Monowarul Mobin Siddique

ASEAN Journal on Science and Technology for Development

Metabolic-associated steatotic liver disease is a metabolic disorder with limited pharmacological treatment options and only one approved medication to date. MASLD can be asymptomatic in its earlier stage and hence, prevention of this disease progression is the most effective approach. Recently, green-synthesized nanoparticles, particularly silver (AgNPs) and gold nanoparticles (AuNPs), have gained interest due to their eco-friendly production, biocompatibility, and bioactive properties. This review highlights the biosynthesis of AgNPs and AuNPs using medicinal plant extracts and their reported biological effects relevant to MASLD management including anti-hyperlipidemic, anti-diabetic, antioxidant and anti-inflammatory activities. While findings are promising, further mechanistic studies and preclinical …

Artificial Intelligence To Transform Lifelong Learning And Workforce Development In Southeast Asia: A Call To Action, Shyh Poh Teo, Khadizah H. Abdul-Mumin

ASEAN Journal on Science and Technology for Development

No abstract for this article type.

An Economic And Production Analysis Of Seaweed Farms In California And The Pacific Northwest, Brian Donovan

Cal Poly Humboldt theses and projects

Since the early 2010s, the seaweed aquaculture industry has steadily expanded in the United States, with states like Alaska, Maine, Hawaii, and Connecticut leading the way in the development of this emerging sector. However, information gaps remain concerning certain aspects of the domestic seaweed aquaculture industry. The purpose of this study was to examine the status of the seaweed aquaculture industry along the West Coast of the United States; specifically in the states of California, Oregon, and Washington. Seaweed farmers in these states were invited to voluntarily complete surveys asking important production, economic, and commercial questions regarding their operations. Data …

Macro-Ergonomic And Organizational Behavior Mapping For Organic Waste Management Using Hofstede Culture In Semarang, Indonesia : Pca & Sem Analysis Using Smart Pls, Ratih Setyaningrum, Sahana Pramukti, Ravindran Balasubramani

ASEAN Journal on Science and Technology for Development

Waste management has a very close and complex relationship with community behavior and culture . This relationship is two-way: community culture influences how waste is managed, and conversely, waste management practices can shape or change culture. This study to assess the organizational behavior related to organic waste management. Employing a quantitative methodology, data were gathered using the Hofstede Values Survey Module 13 (VSM 13) - closed questionnaire, with 109 Semarang city Community participants responding via a Google Form. The collected data were analyzed utilizing Hofstede's prescribed formula. The next stage is PCA (Principal Component Analysis) and SEM (Structural Equation Modeling) …

Development Of Potentiometric Aptasensor For The Detection Of Escherichia Coli, Chalinee Auanphui

Chulalongkorn University Theses and Dissertations (Chula ETD)

A potentiometric aptasensor was developed for the rapid and label-free detection of Escherichia coli (E. coli), a major food- and water-borne pathogen of public health concern. The sensor utilized a laser-induced graphene (LIG) electrode fabricated by laser engraving on a polyimide substrate, followed by modification with an ion marker, coating with a carboxylated poly(vinyl chloride) (PVC–COOH) membrane, and immobilization of an E. coli-specific aptamer. The remaining surface was blocked with bovine serum albumin (BSA) to minimize nonspecific adsorption. Detection was based on changes in ion exchange between the ion marker and membrane upon the specific binding of E. coli to …