Biotechnology Commons^™

Unravelling The Role Of Two-Component Systems In Mycobacterium Abscessus, Andrea C. Molero Perez

Honors Undergraduate Theses

Mycobacterium abscessus (Mab) is an emerging pathogen within the nontuberculous bacteria (NTM) which poses a threat due to its complex multi-drug resistance and opportunistic nature. It is predicted that only 45.6% of patients will have successful treatment, with immunocompromised and cystic fibrosis patients being the most threatened. Moreover, investigating new treatment approaches is key to responding to this public health issue. Two-component systems (TCS) are regulatory pathways that play a pivotal role in bacterial survival and virulence. These systems are composed of a sensor histidine kinase (HK) and cognate response regulator (RR) protein that senses environmental cues and triggers specific …

Multimodal Imaging Of Protein-Based Biomaterials For Delivering Chemo-Agent Cargo For Glioblastoma Treatment In A Murine Model, Orin Mishkit

Dissertations and Theses

Protein-based self-assembling biomaterials, known as Thermo-Responsive Assembled Proteins (TRAP), present meaningful potential as drug delivery carriers. These materials enable the controlled, slow release of poorly soluble chemotherapeutic agents, such as doxorubicin (Dox), while potentially reducing systemic off-target effects. In collaboration with multiple NYU labs, this study evaluated the efficacy of two TRAP variants—TRAP and F-TRAP—as drug delivery carriers in a xenograft mouse model of glioblastoma multiforme (GBM).

The primary objective was to compare the effectiveness of TRAP-loaded Dox (TRAP-DOX) to free Dox in achieving tumor extravasation and accumulation, facilitating sustained drug release. We hypothesized that the leaky vasculature of GBM …

Quality And Safety Analysis Of Integrated Bus Services And Feeder Transport In Surabaya, Indonesia, Desrina Yusi Irawati, Agrienta Bellanov, Armadeo Ruben Canariesa

ASEAN Journal on Science and Technology for Development

The Surabaya city government has developed public transportation through integrated bus services and feeder transport. These transportation systems include Trans Semanggi, Suroboyo Bus, and Wira Wiri Surabaya. The challenge facing these transportation programs is the low level of public participation in using public transportation. This research aims to provide a comprehensive investigation of the perceived service quality of integrated public transportation in Surabaya by combining the RESCA and SERVQUAL frameworks. The study contributes by highlighting service quality gaps across five customized dimensions and offering practical insights to improve user satisfaction and increase public transport usage. Each RESCA dimension incorporates RATER …

Intelligent Biology And Medicine: Accelerating Innovative Computational Approaches, Fuhai Li, Li Liu, Kai Wang, Xiaoming Liu, Zhongming Zhao

Faculty, Staff and Student Publications

In this editorial, we summarize the 2023 International Conference on Intelligent Biology and Medicine (ICIBM 2023) conference which was held on July 16-19, 2023 in Tampa, Florida, USA. We then briefly describe the nine research articles included in this special issue. ICIBM 2023 scientific program included four tutorials and workshops, four keynote lectures, four eminent scholars' presentations, 11 concurrent scientific sessions, and a poster session. We had total of 88 scientific oral presentations, including 62 regular oral presentations and 26 flash talks, as well as 46 poster presentations. The topics of these presentations covered artificial intelligence (AI), data sciences, bioinformatics, …

An Economic And Production Analysis Of Seaweed Farms In California And The Pacific Northwest, Brian Donovan

Cal Poly Humboldt theses and projects

Since the early 2010s, the seaweed aquaculture industry has steadily expanded in the United States, with states like Alaska, Maine, Hawaii, and Connecticut leading the way in the development of this emerging sector. However, information gaps remain concerning certain aspects of the domestic seaweed aquaculture industry. The purpose of this study was to examine the status of the seaweed aquaculture industry along the West Coast of the United States; specifically in the states of California, Oregon, and Washington. Seaweed farmers in these states were invited to voluntarily complete surveys asking important production, economic, and commercial questions regarding their operations. Data …

Longitudinal And Ensemble Modeling Of Beef Cattle Feed And Water Intakes, Nathan El Blake

Graduate Theses, Dissertations, and Problem Reports (ETD)

Beef cattle production remains a cornerstone of global food systems, providing highly bioavailable protein and essential micronutrients that are difficult to secure from plant-based foods alone. Yet this production is also resource-intensive, requiring substantial land, feed, and water inputs while contributing significantly to greenhouse gas emissions. Efficiency of dry matter intake (DMI) and water intake (WI) is central to both productivity and sustainability, but accurate measurement of these traits—particularly in grazing systems—remains a major limitation. National WI standards continue to rely on pen-level data collected in the 1950s, while most existing DMI prediction equations perform poorly when applied beyond the …

Assessing The Probable Sources Affecting The Water Quality Index Of The Panch Prayag Belt Of Uttarakhand, India, Debasis Sau, Indranil Mukherjee, Wasim Akram, Kakali Ghosh, Rimi Paul, Reshmi Paul, Athar Akram

ASEAN Journal on Science and Technology for Development

The Water Quality Index (WQI) is an essential metric for evaluating the usability of surface water resources, particularly in ecologically sensitive and high-demand areas like the Panch Prayag belt of Uttarakhand, India. This region, comprising five major pilgrimage towns—Devaprayag, Nandprayag, Vishnuprayag, Karnaprayag, and Rudraprayag—faces seasonal fluctuations in water quality due to both natural and anthropogenic pressures. In this study, water samples were collected from 2021 to 2023 across pre-monsoon, monsoon, and post-monsoon seasons, and the WQI was computed using the Canadian Water Quality Index (CWQI 1.0). Results revealed that WQI values ranged from 36 to 45 across locations and seasons, …

Macro-Ergonomic And Organizational Behavior Mapping For Organic Waste Management Using Hofstede Culture In Semarang, Indonesia : Pca & Sem Analysis Using Smart Pls, Ratih Setyaningrum, Sahana Pramukti, Ravindran Balasubramani

ASEAN Journal on Science and Technology for Development

Waste management has a very close and complex relationship with community behavior and culture . This relationship is two-way: community culture influences how waste is managed, and conversely, waste management practices can shape or change culture. This study to assess the organizational behavior related to organic waste management. Employing a quantitative methodology, data were gathered using the Hofstede Values Survey Module 13 (VSM 13) - closed questionnaire, with 109 Semarang city Community participants responding via a Google Form. The collected data were analyzed utilizing Hofstede's prescribed formula. The next stage is PCA (Principal Component Analysis) and SEM (Structural Equation Modeling) …

Development Of Potentiometric Aptasensor For The Detection Of Escherichia Coli, Chalinee Auanphui

Chulalongkorn University Theses and Dissertations (Chula ETD)

A potentiometric aptasensor was developed for the rapid and label-free detection of Escherichia coli (E. coli), a major food- and water-borne pathogen of public health concern. The sensor utilized a laser-induced graphene (LIG) electrode fabricated by laser engraving on a polyimide substrate, followed by modification with an ion marker, coating with a carboxylated poly(vinyl chloride) (PVC–COOH) membrane, and immobilization of an E. coli-specific aptamer. The remaining surface was blocked with bovine serum albumin (BSA) to minimize nonspecific adsorption. Detection was based on changes in ion exchange between the ion marker and membrane upon the specific binding of E. coli to …

Cgrp Modulation Of Intracellular Calcium Levels In Trigeminal Ganglion Neurons And Glia, Nicole M. Nalley

Graduate Theses/Dissertations

Elevated levels of calcitonin gene-related peptide (CGRP) are implicated in migraine and TMD pathology, but its effects on the excitability state of Aδ and C-fiber neurons and glia have not been investigated. The goal of this study was to determine changes mediated by CGRP on intracellular calcium levels in trigeminal ganglion neurons and glia in response to depolarizing stimulation. Intracellular calcium levels were determined in Aδ and C-fiber neurons of primary trigeminal ganglion cultures obtained from neonatal Sprague Dawley rats using Fura-2 and fluorescent microscopy. Cells were left untreated or preincubated for 2 hours with CGRP, then incubated with the …

A Pyrrole Modified 3,4-Propylenedioxythiophene Conjugated Polymer As Hole Transport Layer For Efficient And Stable Perovskite Solar Cells, Yuanhao Tang, Ke Ma, Wenhao Shao, Yoon Ho Lee, Ashkan Abtahi, Jiaonan Sun, Hanjun Yang, Aidan H. Coffey, Harindi R. Atapattu, Mustafa Ahmed, Qixuan Hu, Wenzhan Xu, Raunak Dani, Limei Wang, Chenhui Zhu, Kenneth R. Graham, Jianguo Mei, Letian Dou

Chemical and Materials Engineering Faculty Publications

Despite the outstanding electric properties and cost-effectiveness of poly- (3,4-ethylenedioxythiophene) (PEDOT) and its derivatives, their performance as hole transport layer (HTL) materials in conventional perovskite solar cells (PSCs) has lagged behind that of widely used spirobifluorene-based molecules or poly(triaryl amine). This gap is mainly from their poor solubility and energy alignment mismatch. In this work, the design and synthesis of a pyrrole-modified HTL (PPr) based on 3,4-propylenedioxythiophene (ProDOT) are presented for efficient and stable PSCs. As a result of the superior defects passivation ability, excellent contact with perovskite, enhanced hole extraction, and high hydrophobicity, the unencapsulated PPr-based PSCs showed the …

Evaluation Of The Dnazyme Gr5 For The Determination Of Lead Speciation In Natural Waters, Gaganprit Gill

Theses and Dissertations (Comprehensive)

This thesis explores the development and application of a DNAzyme-based biosensor designed to detect labile metal species in environmental samples. Real time and on-site monitoring of labile metal fractions would make a valuable contribution to environmental management, as these fractions are the most bioavailable and pose significant toxicity risks to aquatic organisms. Conventional methods for detecting labile metals, while effective, are often burdened by high costs, complexity, and lengthy processing times, making them less ideal for rapid and widespread environmental assessments.

The first manuscript of this thesis (chapter 2) establishes the fundamental capabilities of the Pb²⁺-specific DNAzyme GR5 …

Revolutionizing Grapevine Breeding Programs: A Mobile Genome Editing System For Perennial Crops, Bridget Bolt

Theses and Dissertations--Plant and Soil Sciences

Grapevine (Vitis vinifera) faces unprecedented challenges from climate change, with up to 90% of current growing regions potentially becoming unsuitable for production within decades. Traditional breeding approaches require 25-30 years for variety development, creating a time constraint incompatible with the pace of environmental change. This research establishes the approach for a novel mobile RNA-based gene editing platform that addresses longstanding bottlenecks in grapevine improvement while preserving cultivar identity and reducing breeding timelines.

This research developed a three-component system for transgene-free gene editing in grapevine. First, a mobile CRISPR/Cas9 system was established using Agrobacterium rhizogenes transformation and the cut-and-dip …

From Theory To Practice: Simulating Zero Trust Architecture With Python For Cybersecurity, Pankaj R. Chandre, Bhagyashree D. Shendkar, Sulochana Sagar Madachane, Nikita Kulkarni, Sayalee Deshmukh

ASEAN Journal on Science and Technology for Development

The increasing complexity and frequency of cyber threats have necessitated the adoption of advanced security models, such as Zero Trust Architecture (ZTA). ZTA runs on the tenet that no entity whether inside or outside the network should be trusted by default, in contrast to conventional perimeter-based security solutions. This paper describes a thorough method for simulating and implementing Zero Trust Architecture in Python. We examine the essential elements and procedures of ZTA, such as identity verification, access control, and continuous monitoring, through a thorough analysis and step-by-step manual. The simulation shows how ZTA works to ensure strong network security and …

Regulation Of Haemodynamic Variables By Laguerre Polynomials Based Model Predictive Control., Sai Sandeep Boda, Hiren Kumar G. Patel, Khyati D. Mistry

ASEAN Journal on Science and Technology for Development

Purpose: Cardiac output (CO) and Mean Arterial Pressure (MAP) are two haemodynamic variables that need regulation in clinical situations, particularly in post-cardiac surgery patients. As it is difficult for clinical personnel to monitor continuously, automatic drug infusion to regulate MAP and CO is recommended and essential in the medical environment. Methods: Two input and two output patient model was used in this work to study the variation of haemodynamic variables in response to the infusion of Dopamine (DP) and Sodium Nitroprusside (SNP) drugs. Model predictive control (MPC) algorithm was exercised here as it was a well-established technique to regulate output …

Neuro-Symbolic Ai: A Future Of Tomorrow, Pankaj Chandre, Parikshit Mahalle, Gitanjali Shinde, Bhagyashree Shendkar, Shraddha Kashid

ASEAN Journal on Science and Technology for Development

Neuro-Symbolic AI: A Future of Tomorrow" explores the convergence of neural learning and symbolic reasoning to advance artificial intelligence (AI) systems. Symbolic reasoning makes use of knowledge representation techniques and rule-based systems, whereas neural learning analyzes data using deep learning models. AI becomes more adept at fusing data-driven insights with deductive reasoning when these methods are integrated using hybrid models and differentiable reasoning techniques. Applications show enhanced diagnostic precision and decision-making skills in a variety of industries, including robotics, healthcare, and finance. To promote responsible AI development, regulatory frameworks and ethical principles address issues like bias and transparency. Future paths …

Solid Waste Management Strategies In Coastal Areas Affected By Seawater Flood Disaster, Adib Khoirul Anas, Maryono Maryono, Hartuti Purnaweni

ASEAN Journal on Science and Technology for Development

Solid waste in areas affected by seawater floods must be handled specifically because people often dispose of solid waste on flooded land. This research aims to identify the performance of the community to manage solid waste and solid waste management strategy, solid waste measurement using SNI method 19-3964-1994. Analysis of land affected by seawater floods shows that 12 villages are flooded. Communities in coastal areas are not optimal in managing solid waste because the government has never given directions to manage solid waste in a good way, the facilities and marketing are inadequate so that solid waste is often not …

The Influence Of The Digital Economy On The Ecological Environment And Its Sustainable Development, Xinpan Lyu, Jianhua Dai

ASEAN Journal on Science and Technology for Development

This study aims to examine the impact of the digital economy on ecological sustainability and its development in China from 2000 to 2022. The study focuses on the role of innovation in the digital sector, where the adoption of big data technologies is considered, with reference to sustainable development, from an environmental perspective. A dynamic threshold model and, in particular, the Iterated Generalized Least Squares (IGLS) technique is used to analyze the given data.The research reveals a significant link between adopting digital technologies and managing environmental sustainability efficiently. To investigate the interconnection between the digital economy and ecological goals, it …

Exploring Dynamics And Effective Strategies For Tidal Flood Risk Reduction In Indonesia's Coastal Cities, Satria Yudha Adhitama, Diana Puspitasari, Lucia Sandra Budiman, Azis Musthofa

ASEAN Journal on Science and Technology for Development

Indonesia’s coastal regions have distinct charateristics. However, nearly all of these coastal regions are vulnerable to tidal floods. The purpose of this study is to determine the features of coastal regions in Indonesia that have been damaged by tidal floods, as well as the actions implemented to mitigate disaster risk. Thus study takes a qualitiative method with explanatory analysis. The Island Spatial Planning is used to classify Indonesia’s archipelagic regions.An urban area vulnerable to tidal flood was selected from each archipelagic region. The characteristics of coastal regions affected by tidal flood were identified by the tidal flood characteristics, coastal physiography. …

Analysis Of The Sustainability Management Of Coral Reef Ecosystem In The Marine Protected Area Of Wediombo, Gunungkidul, Nabilah L. Rohmah, Rika Harini, Sudrajat Sudrajat

ASEAN Journal on Science and Technology for Development

The Wediombo Coastal Area is a Marine Protected Area (MPA) with highly potential in coral reef ecosystems. According to the Millennium Ecosystem Assessment (MEA), coral reefs play a vital role in supporting human life by providing provisioning, supporting, regulating, and cultural services. However, the utilization of this area as a tourist destination and suboptimal management practices have led to the degradation of its coral reef ecosystems. Efective management strategies for the sustainability of coral reefs can have positive impacts across ecological, economic, social, technological, and institutional dimensions. Therefore, this study aims to analyze the sustainability management of coral reef ecosystem …