Biotechnology Commons^™

The Use Of Ethyl Lauroyl Arginate Encapsulated In Organic Nanoparticles Within Boar Semen Extenders To Control Bacterial Growth And Preserve Sperm Quality During Short-Term Storage Without Antibiotics, Congbang Ngo, Naiyaphat Nittayasut, Teerapong Yata, Junpen Suwimonteerabutr, Nuvee Prapasarakul, Jane M. Morrell, Padet Tummaruk

The Thai Journal of Veterinary Medicine

The aim of the present study was to investigate the effect of adding ethyl lauroyl arginate (ELA), encapsulated in organic nanoparticles, to a semen extender without antibiotics on sperm quality and bacterial load during short-term storage. Three ejaculates from Duroc boars were divided into four equal portions and diluted with Beltsville Thawing Solution to produce semen doses containing 3 billion spermatozoa in 80 ml. The extended semen did not include antibiotics but instead incorporated different concentrations of ELA nanoparticles into the extenders: 0 ppm, 5 ppm, 10 ppm, and 15 ppm. The semen doses were stored for 4 days, with …

Fta™ Card Use For Detection Of Mycoplasma Gallisepticum In Wild Birds, Linda Purvis, Dawn E.W. Drumtra

Georgia Journal of Science

Flinders Technology Associates (FTA) cards are increasingly accepted as an accurate method for nucleic acid detection, including testing for the presence of various avian diseases. However, the accuracy of FTA cards for detection of Mycoplasma gallisepticum (MG) in wild birds has not been published. Traditional techniques for testing MG in birds include serum plate agglutanation (SPA), hemagglutination inhibition (HI), polymerase chain reaction (PCR), and enzyme linked immunosorbent assay (ELISA) all of which are time consuming and require significant financial investment into specialized equipment. We tested the efficacy of using FTA cards to detect MG in wild bird carcasses collected in …

Characterization Of The Slc7a5/Slc3a2 Amino Acid Transporter In Human Pleural Mesothelial Cells, Ann Francis

Biotechnology Theses

The SLC7A5/SLC3A2 amino acid transporter moves essential amino acids, such as leucine, phenylalanine, and tryptophan, across the cellular plasma membrane. SLC7A5 overexpression was identified in diverse cancers, suggesting a role in tumorigenesis and cancer cell proliferation. However, the role of SLC7A5/SLC3A2 in other aggressive cellular phenotypes, including mesothelial mesenchymal transition (MesoMT), remains unexplored. RNA sequencing analyses identified SLC7A5 as one of three highly upregulated SLCs in mesothelial cell derived myofibroblasts. This sodium independent transporter exhibits a high affinity for branched chain amino acids such as leucine; however, what role, if any, this channel plays in MesoMT is unclear. SLC7A5 knockdown …

Explorations Of Dna-Single-Walled Carbon Nanotube Interactions To Develop Multiplexed Molecularly Specific Biosensors For Inflammation, Amelia K. Ryan

Dissertations and Theses

Inflammatory cytokines such as interleukin-6 (IL-6) and interleukin-12 (IL-12) are central regulators of immune signaling and key biomarkers of disease, yet existing assays for their detection remain slow, invasive, and lack multiplexing ability. This dissertation advances the development of single-walled carbon nanotube (SWCNT) optical nanosensors capable of real-time, multiplexed, and molecularly specific cytokine detection through innovative use of single-stranded DNA (ssDNA) interfaces.

First, an IL-6-specific DNA aptamer was employed as both a dispersing agent and recognition probe for SWCNT fluorescence sensing. Sequence modifications, including anchor domains, truncations, and thermally induced refolding, were systematically tested to optimize sensitivity and selectivity. The …

Genetic Property Rhetoric And The Public Domain, Dave Fagundes

Texas A&M Journal of Property Law

Among the many themes that illuminate Jorge Contreras’s The Genome Defense, this Essay focuses on property. It considers the rhetorical role played by property in convincing both courts and the public that patenting genetic data was legally and ethically objectionable. This Essay begins by locating property—and public property—as a major theme in the dialogue surrounding the Myriad litigation. It then situates this rhetorical move in the context of property-talk generally, highlighting the distinctiveness of leveraging public rather than private ownership as a means of accessing moral intuitions. Finally, this Essay reflects on what the use of public property rhetoric …

Optimization Of Transformation And Regeneration In The Sunflower (Helianthus Annuus), Sara M. Kilar

Masters Theses

In addition to their value as prized ornamentals, sunflowers are important crop species due to the nutritional value and high oil content of the seeds. Sunflower oil is a significant contributor to global seed oil production, which can be used as a food additive and cooking agent as well as a raw material for biodiesel production. While traditional breeding efforts have led to the development of many unique cultivars, genetically modified variations have proven more difficult due to the recalcitrance of sunflower plants to genetic transformation. As a result, facile and efficient transformation methods for sunflowers have the potential to …

Functional Analysis Of Ligand Binding Domain 2 Of Outer Surface Protein C Of Borreliella Burgdorferi, Gavin Z. Chambers

Theses and Dissertations

Lyme disease, caused by the spirochete Borreliella burgdorferi, is the most common vector-borne infection in North America. A critical determinant of early mammalian infection is outer surface protein C (OspC), a highly variable lipoprotein that is indispensable for spirochete survival but whose precise function remains unresolved. OspC has been proposed to mediate diverse roles, including complement evasion, adhesion, tissue dissemination, and antiphagocytic activity, supporting the view that it is a multifunctional virulence factor. Structural studies have identified two putative ligand-binding domains (LBD1 and LBD2), with LBD2 forming a solvent-exposed crown at the membrane-distal end of the OspC dimer.

To …

Enhancing The Mechanical Properties Of Sla 3d-Printed Bio-Resin From Linseed Oil Using Cellulose And Lignin Biopolymers, Chukwunonso A. Ikedionu

College of Graduate Studies: Theses & Dissertations

Abstract

This research explores the development of bio resin from linseed oil by the process of epoxidation and acrylation to produce acrylated epoxidized linseed oil (AELO). Lignin and cellulose biopolymers were added as reinforcement at varying weight fractions of 1 wt.%, 2.5 wt.%, and 5 wt.%, and their mechanical performance was compared against pure AELO. The formulations were printed using an Elegoo SLA 3D printer to create standardized test specimens like tensile bars, compression cylinders, and hardness blocks, which were all prepared according to the ASTM testing standard.

The results indicate that reinforcement effects depend on both filler type and …

Ribomoe: An Application Of Mixture Of Experts On Artificial Riboswitch Classification, Hainian Audrey Long

Master's Projects

Urban water sewage is a potential health concern due to its possibility to spread contagious RNA viruses such as Coxsackievirus B3. However, detection of viral particles remains challenging because of low viral concentrations in wastewater and high mutation rates of the RNA virus. To address this, this study proposes a novel viral detection method using synthetic riboswitches that bind to the target virus and trigger a reporter gene, amplifying the detection signals. To support the design of effective riboswitches, we present a machine learning model for classifying riboswitch performance, integrating RNA sequence data with secondary structural features. This model used …

Every Publication Needs An Author Verified Tl;Dr Statement, Sarah Oerther, Daniel B. Oerther

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

No abstract provided.

Leveraging Strategic Information Systems For Competitive Advantage: A Case Study Of Veolia As A Multinational Enterprise, Sohail Nawaz Sabir

ASEAN Journal on Science and Technology for Development

Instead of examining several components that link planning and strategy implementation, researchers have focused on the alignment of IT and business, as well as the Strategic Information Systems Planning (SISP) process. The goal of this study is to examine several approaches that can close the gap between IT project planning and execution. Therefore, the primary objective of the proposed study is to examine SISP implementation strategies that can be theorized in the context of Veolia, a global multinational corporation (MNC). The primary aspects influencing the SISP for this study are the frequency of planning, the function of IT/business managers, assessment …

Potential Of Green Synthesis Of Silver And Gold Nanoparticles For The Management Of Metabolic Associated Steatotic Liver Disease: A Mini Review, Atiqah Suhaili, Ya Chee Lim, Mei Ann Lim, Lee Hoon Lim, Mohammad Mansoob Khan, Monowarul Mobin Siddique

ASEAN Journal on Science and Technology for Development

Metabolic-associated steatotic liver disease is a metabolic disorder with limited pharmacological treatment options and only one approved medication to date. MASLD can be asymptomatic in its earlier stage and hence, prevention of this disease progression is the most effective approach. Recently, green-synthesized nanoparticles, particularly silver (AgNPs) and gold nanoparticles (AuNPs), have gained interest due to their eco-friendly production, biocompatibility, and bioactive properties. This review highlights the biosynthesis of AgNPs and AuNPs using medicinal plant extracts and their reported biological effects relevant to MASLD management including anti-hyperlipidemic, anti-diabetic, antioxidant and anti-inflammatory activities. While findings are promising, further mechanistic studies and preclinical …

Investigating The Role Of Prostasin In Angiogenesis: A Chorioallantoic Membrane Model Approach, Zaima Aline

Honors Undergraduate Theses

Prostasin, also known as PRSS8 or CAP1, is a glycosylphosphatidylinositol (GPI)-anchored extracellular serine protease involved in various physiological and pathophysiological processes. Prostasin expression is observed during key phases of trophoblast invasion and placental development, critical steps in embryo implantation in Rhesus monkeys. This suggests a role for prostasin in extracellular matrix remodeling and tissue invasion, both essential for a healthy pregnancy. Moreover, prostasin appears vital for development as studies in mice show that a germline knockout of the gene leads to significant placental defects and eventual embryonic death, highlighting its importance in vascular and structural development within the placenta.

Building …

การวิเคราะห์เมตาโบโลมิกส์ในน้ำนมเหลืองและน้ำนมจากแม่สุกรที่ได้รับอาหารเสริมซินไบโอติก, เมธาพร เจือจันทร์

Chulalongkorn University Theses and Dissertations (Chula ETD)

ปัจจุบันมีการใช้สารเสริมอาหารสัตว์ในกลุ่มจุลินทรีย์โพรไบโอติกและซินไบโอติกเพื่อทดแทนการใช้สารปฏิชีวนะและลดปัญหาการเกิดเชื้อดื้อยาในอุตสาหกรรมการผลิตสุกร ซึ่งสารเสริมชีวนะดังกล่าวส่งผลในเชิงการส่งเสริมสุขภาพของแม่สุกร เพิ่มประสิทธิภาพในการสังเคราะห์องค์ประกอบและสารภูมิคุ้มกันในน้ำนม รวมทั้งช่วยลดอัตราการเกิดโรคท้องเสียในลูกสุกร อย่างไรก็ตาม การศึกษาข้อมูลสารชีวโมเลกุลขนาดเล็กหรือสารเมตาบอไลต์ในน้ำนมจากแม่สุกรที่ได้รับอาหารเสริมซินไบโอติกยังมีอยู่อย่างจำกัด ดังนั้น งานวิจัยนี้จึงมีวัตถุประสงค์เพื่อวิเคราะห์และเปรียบเทียบข้อมูลสารชีวโมเลกุลในน้ำนมจากแม่สุกรที่ได้รับอาหารที่มีการเสริมซินไบโอติก โดยพิจารณาร่วมกับอิทธิพลของระยะการให้นมและอิทธิพลจากลำดับครอกที่แตกต่างกันของแม่สุกร ผลการวิจัยพบว่าสามารถระบุชนิดของสารเมตาบอไลต์และกรดไขมันในตัวอย่างน้ำนมสุกรได้ทั้งหมด 47 และ 23 สาร โดยใช้เทคนิค 1H-NMR และ GC-FID ตามลำดับ ผลการวิเคราะห์ทางสถิติพหุตัวแปรแสดงให้เห็นว่าอิทธิพลจากระยะการให้นมส่งผลต่อการเปลี่ยนแปลงข้อมูลสารชีวโมเลกุลในน้ำนมสุกรอย่างชัดเจน เมื่อพิจารณาตัวอย่างน้ำนมที่เก็บในระยะเดียวกัน พบว่าอิทธิพลของการได้รับอาหารเสริมซินไบโอติกส่งผลต่อความแตกต่างของข้อมูลสาร เมตาบอไลต์ในตัวอย่างน้ำนมเหลืองและน้ำนมในวันที่ 10 เท่านั้น แต่อิทธิพลดังกล่าวส่งผลต่อการเปลี่ยนแปลงข้อมูลกรดไขมันตลอดทุกช่วงระยะการให้นม โดยสามารถใช้การเปลี่ยนแปลงปริมาณสัมพัทธ์ของ adenine, hypoxanthine, taurine, linolenic acid, linoleic acid และ lauric acid เป็นตัวบ่งชี้ทางชีวภาพเพื่อระบุความแตกต่างของน้ำนมจากแม่สุกรที่ได้รับอาหารเสริมซินไบโอติกได้ ทั้งนี้อิทธิพลจากลำดับครอกของแม่สุกรไม่ส่งผลต่อความแตกต่างของข้อมูลสารเมตาบอไลต์ แต่ส่งผลต่อความแตกต่างของข้อมูลกรดไขมันอย่างชัดเจนตลอดทุกช่วงระยะการให้นม โดยเฉพาะในแม่สุกรกลุ่มที่ได้รับอาหารเสริมซินไบโอติก ผลการวิจัยนี้แสดงให้เห็นถึงการประยุกต์ใช้เทคโนโลยีเมตาโบโลมิกส์ในการศึกษาการเปลี่ยนแปลงข้อมูลสารชีวโมเลกุลในน้ำนมสุกร และระบุชนิดของตัวบ่งชี้ทางชีวภาพต่ออิทธิพลของการได้รับอาหารเสริมซินไบโอติกได้อย่างมีประสิทธิภาพ

Leukocyte Infiltration Profiles At The Site Of Intradermal Injection Of Silikon-1000 In Scleroderma-Prone Ucd200/206 And Healthy White Leghorn Chickens, Katee L. Johnson, Jossie M. Santamaria, Chrysta N. Beck, Gisela F. Erf

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Intradermal injections of silicone-based fillers for the face and body may cause chronic inflammation and fibrotic reactions in some individuals. Chickens from the UCD 200/206 White Leghorn (WL) line spontaneously develop a fibrotic autoimmune disease known as scleroderma/systemic sclerosis (SSc). Using the SSc-prone UCD and healthy WL chickens, a longitudinal study was conducted to examine the cellular inflammatory responses to intradermal (i.d.) injections of sterile SILIKON-1000. Specifically, the pulp (a skin derivative) of growing feathers (GFs) was i.d. injected with sterile SILIKON-1000 or phosphate-buffered saline (PBS) as injection control (8 and 3 birds/line, respectively; 10 µL/GF; 20 GFs/bird). Two GFs …

A Novel Silver-Ruthenium-Based Antimicrobial Kills Gram-Negative Bacteria Through Oxidative Stress-Induced Macromolecular Damage, Patrick O. Tawiah

Theses and Dissertations

The rise in drug-resistant bacteria, together with the decline in antibiotic development, requires new strategies for infectious disease control. Gram-negative pathogens are particularly challenging to combat due to their outer membrane. This study highlights the effectiveness of the silver-containing antimicrobial AGXX® against the Gram-negative bacterium Escherichia coli. AGXX® is a promising silver coating that presumably kills bacteria through the generation of reactive oxygen species (ROS). However, neither AGXX®’s mode of action is fully understood, nor have its effects on Gram-negative bacteria or bacterial response and defense mechanisms towards AGXX® been studied in detail. Here, I report that the bactericidal …

Minimizing Cpu Utilization For Job Scheduling Problems By The Advanced Round Robin Method: A Pragmatic Perspective, Haribhau R Bhapkar, Pankaj R Chandre, Parikshit Mahalle

ASEAN Journal on Science and Technology for Development

CPU scheduling issues include minimizing waiting time for processes, ensuring fairness in resource allocation, and optimizing throughput. Balancing these objectives can be challenging, as improving one aspect may negatively impact another, making it essential to design efficient scheduling algorithms. This work presents an innovative approach to enhance CPU utilization through the development of a Result-Based Round Robin Scheduling Algorithm. Traditional Round Robin Scheduling methods often face challenges in efficiently allocating CPU time, leading to suboptimal system performance. In response to these limitations, the proposed method introduces a result-oriented strategy that dynamically adjusts time quantum allocations based on the execution progress …

Engineering Geological And Groundwater Mapping Of Negara Brunei Darussalam For Urban Planning, Nur Thaqifah Junaidi, Siti Lieyana Azffri, Afiq Aiman Jaya, Muhammad Asri Akmal Suhip, Stefan Herwig Gödeke

ASEAN Journal on Science and Technology for Development

Engineering geology and Hydrogeology play a crucial role in the development of urban areas. Without a thorough geological and hydrogeological understanding, urban development can be risky and prone to numerous challenges. In this study, we aim to conduct engineering geological and groundwater mapping of Brunei utilizing data from 614 sacrificial investigative boreholes drilled over the course of 40 years for geotechnical investigation and housing development. Borehole parameters such as soil data, SPT N-values, depth to groundwater and surface elevation were gathered and evaluated. Soil investigation in the study area revealed predominantly clayey sediments with SPT N-values ranging from 0 to …

The Effect Of Blanching And Steaming Pretreatment On The Quality Of Durian (Durio Zibenthinus Murr.) Seed Flour, Nabilah Daniel, Rosnah Shamsudin, Mohd Sabri Pak Dek, Jhauharotul Muchlisyiyah, Masmunira Rambli

ASEAN Journal on Science and Technology for Development

Durian (Durio zibethinus Murr.), the "King of Fruits," has seeds that offer nutritional benefits, including high fiber for gut health and high protein for muscle growth. However, natural browning enzymes can limit its use in baking. This study investigates the comparative effects of blanching and steaming durations (5, 10, 15, 20, and 25 minutes) on the color and techno-functional properties of durian seed flour (DSF), providing an optimization-based approach rarely reported for this material.. Durian seeds were sourced from various stalls in Malaysia, washed, soaked in 0.6% sodium metabisulfite, and then blanched or steamed for for varying durations as …

Optimization Of L-Citrulline Production In Corynebacterium Glutamicum, And The Effect Of The Human Small Intestine Environment, Simran S. Ghulldu

Honors Undergraduate Theses

L-citrulline is a crucial amino acid involved in nitric oxide production, which supports cardiovascular health, physical performance, and intestinal well-being. Nitric oxide deficiency is linked to conditions like hypertension, highlighting the need for efficient L-citrulline production. This study attempts to enhance L-citrulline synthesis in Corynebacterium glutamicum, a bacterium widely used in industrial amino acid production, as well as test a The hypothesis is that overexpressing carAB, which encodes carbamoyl phosphate synthetase, will increase carbamoyl phosphate levels and boost L-citrulline production. Two C. glutamicum strains were used: a wild type and the CRBG strain, which has mutations that promote …