Biotechnology Commons^™

A Double-Sided Hybrid Lc Series/Parallel Compensation Topology For An Efficient Magnetic Resonance Wireless Power Transfer Systems, Talha Irshad, Malik Nauman, Pg Emeroylariffion Abas

ASEAN Journal on Science and Technology for Development

Conventional plug-in charging faces numerous challenges due to radiation hazards, inconvenience, etc. To mitigate the challenges conventional charging methods face, wireless power transfer (WPT) systems emerged as an efficient method for wireless energy transmission across various applications such as electric vehicles, biomedical implants, smartphones, and network sensors. A robust WPT system needs to perform exceptionally well under varying conditions, however, the WPT performance is reduced due to the leakage inductance between the coils. To address this challenge, compensation networks are often employed to minimize the losses and enhance the overall performance of the WPT system. This paper focuses on designing …

Let’S Be Cool About It: Anticipating Proper, Whole-Human Cryopreservation And The Necessary Oversight For Adequate Consumer Protection, Peter Lyon

Minnesota Journal of Law, Science & Technology

No abstract provided.

Agile Project Management And The Future Of R&D, Aditi Vinayak Nalavade

Harrisburg University Dissertations and Theses

The use of Agile methodologies in research and development (R&D) sectors is changing the project management landscape by profoundly altering approaches to innovation and execution. This research will delve into the benefits and challenges of implementing Agile in an R&D setting, with a particular focus on the increasing significance of AI. The research's central theme offers a comprehensive analysis of how Agile approaches are shaping the future of R&D. Agile's iterative process, with its high degree of adaptability, flexibility, and iterative methods, is particularly beneficial in R&D, where reshaping research teams' approaches to creativity and problem-solving even in the most …

Identification Of Novel Argonaute Proteins Using A Metagenomic Mining Approach, Lobna Abdallah Ghonaim

Theses and Dissertations

Gene editing is one of the most promising tools in science. It enables precise modifications of an organism's genetic material. Metagenomics is considered a powerful tool that unlocks the broad genetic potential found in uncultured microbial communities. Exploring the genetic diversity of uncultured microbial communities helps identify novel functional proteins with unique properties and make the best use of these diverse microbial ecosystems.

We developed and employed a metagenomic-based approach to mine more than 1000 metagenomes for prokaryotic argonaute proteins (pAgos), a potential gene editing machinery encoded in bacterial and archaeal genomes. Our workflow involved strict quality control, sequence assembly, …

Identification Of Novel Genetic Biomarkers Linked To The Prodromal Stage Of Parkinson’S Disease (Pd) Patients, Marwa Tawfik Badawy

Theses and Dissertations

Background and Objective: The early diagnosis of neurodegenerative diseases, such as Parkinson's disease (PD), is particularly challenging because symptoms appear only after significant neuronal damage has already occurred. This study is utilizing variant call format (VCF) analysis to identify genetic variants and novel genes that could serve as early prognostic markers for prodromal PD. Materials and Methods: Data were sourced from the Parkinson's Progression Markers Initiative (PPMI), focusing on prodromal patients with gVCF data from the 2021 cohort. The study included 304 participants, comprising 100 healthy controls, 146 individuals with prodromal genetic indicators, 21 individuals with prodromal hyposmia, and 37 …

Strength And Durability Characteristics Of Mortar Containing Fine Glass Powders As Partial Substitute Of Cement, Deepa Paul, Bindhu K.R, G. M. Sadiqul Islam, Bindhu K. R.

ASEAN Journal on Science and Technology for Development

A recent trend in construction involves the application of high-strength mortar which consumes a high quantity of cement. The present study aims at producing a sustainable mortar utilising waste glass powders (GP) as a partial substitute for cement in mortar. GP grains having different particle sizes in equal proportions were carried out in the mortar mix for improved packing. After realising the cementing properties of GP particles from the microanalyses like micron-size, silica-rich, reactive, and thermal resistance, mechanical and durability aspects of the mortar composite with manufactured sand as a fine aggregate were examined. The mechanical properties of the mortar …

The Role Of Gsk-3Α Inhibition In Attenuating Lps-Induced Inflammation In Raw 264.7 Cells, Oluwaseyi Oyewole

Theses and Dissertations

Glycogen synthase kinase-3 (GSK-3), an enzyme that modulates glucose metabolism, has been recognized as an essential target in major inflammatory diseases based on its great specificity in substrate recognition. GSK-3β has been connected to cancer, obesity, liver diseases, and neurological diseases. Nonetheless, the other GSK-3 isoform, GSK-3α, is much less studied. Previous studies from our laboratory showed that inhibiting GSK-3α potentially confers an anti-inflammatory response when tested on microglia. However, little has been documented regarding the exact mechanism by which GSK-3α exerts its effect. One of the potential mechanisms involving its anti-inflammatory effect could be partially attributed to activating the …

Immuno-Bioinformatics Study Of Secondary Metabolites From Watercress Nasturtium Officinale As Immunostimulant In Whiteleg Shrimp Litopenaeus Vannamei By Targeting Peroxinectin And Scavenger Receptor Class B, Qurrota A’Yunin, Muhaimin Rifa’I, Maftuch Maftuch, Yoga Dwi Jatmiko, Muhammad Hermawan Widyananda, Triyanto Triyanto, Muhammad Tegar Handrianto

Karbala International Journal of Modern Science

The viral disease affects the whiteleg shrimp (Litopenaeus vannamei) and causes losses. Immunostimulants are recognized as a more environmentally friendly approach to disease management and antimicrobial properties of aquatic organisms. Watercress (Nasturtium officinale) is an aquatic plant species that contains antioxidant properties that can protect the organism from disease and boost the immune system. Shrimp rely heavily on innate immunity to combat infectious agents due to their absence of adaptive immunity. Peroxinectin and scavenger receptor class B (SRB) play essential roles in the shrimp defense system. This research employs a bioinformatic to assess the immunostimulatory potential …

The Use Of Ethyl Lauroyl Arginate Encapsulated In Organic Nanoparticles Within Boar Semen Extenders To Control Bacterial Growth And Preserve Sperm Quality During Short-Term Storage Without Antibiotics, Congbang Ngo, Naiyaphat Nittayasut, Teerapong Yata, Junpen Suwimonteerabutr, Nuvee Prapasarakul, Jane M. Morrell, Padet Tummaruk

The Thai Journal of Veterinary Medicine

The aim of the present study was to investigate the effect of adding ethyl lauroyl arginate (ELA), encapsulated in organic nanoparticles, to a semen extender without antibiotics on sperm quality and bacterial load during short-term storage. Three ejaculates from Duroc boars were divided into four equal portions and diluted with Beltsville Thawing Solution to produce semen doses containing 3 billion spermatozoa in 80 ml. The extended semen did not include antibiotics but instead incorporated different concentrations of ELA nanoparticles into the extenders: 0 ppm, 5 ppm, 10 ppm, and 15 ppm. The semen doses were stored for 4 days, with …

Fta™ Card Use For Detection Of Mycoplasma Gallisepticum In Wild Birds, Linda Purvis, Dawn E.W. Drumtra

Georgia Journal of Science

Flinders Technology Associates (FTA) cards are increasingly accepted as an accurate method for nucleic acid detection, including testing for the presence of various avian diseases. However, the accuracy of FTA cards for detection of Mycoplasma gallisepticum (MG) in wild birds has not been published. Traditional techniques for testing MG in birds include serum plate agglutanation (SPA), hemagglutination inhibition (HI), polymerase chain reaction (PCR), and enzyme linked immunosorbent assay (ELISA) all of which are time consuming and require significant financial investment into specialized equipment. We tested the efficacy of using FTA cards to detect MG in wild bird carcasses collected in …

Characterization Of The Slc7a5/Slc3a2 Amino Acid Transporter In Human Pleural Mesothelial Cells, Ann Francis

Biotechnology Theses

The SLC7A5/SLC3A2 amino acid transporter moves essential amino acids, such as leucine, phenylalanine, and tryptophan, across the cellular plasma membrane. SLC7A5 overexpression was identified in diverse cancers, suggesting a role in tumorigenesis and cancer cell proliferation. However, the role of SLC7A5/SLC3A2 in other aggressive cellular phenotypes, including mesothelial mesenchymal transition (MesoMT), remains unexplored. RNA sequencing analyses identified SLC7A5 as one of three highly upregulated SLCs in mesothelial cell derived myofibroblasts. This sodium independent transporter exhibits a high affinity for branched chain amino acids such as leucine; however, what role, if any, this channel plays in MesoMT is unclear. SLC7A5 knockdown …

Explorations Of Dna-Single-Walled Carbon Nanotube Interactions To Develop Multiplexed Molecularly Specific Biosensors For Inflammation, Amelia K. Ryan

Dissertations and Theses

Inflammatory cytokines such as interleukin-6 (IL-6) and interleukin-12 (IL-12) are central regulators of immune signaling and key biomarkers of disease, yet existing assays for their detection remain slow, invasive, and lack multiplexing ability. This dissertation advances the development of single-walled carbon nanotube (SWCNT) optical nanosensors capable of real-time, multiplexed, and molecularly specific cytokine detection through innovative use of single-stranded DNA (ssDNA) interfaces.

First, an IL-6-specific DNA aptamer was employed as both a dispersing agent and recognition probe for SWCNT fluorescence sensing. Sequence modifications, including anchor domains, truncations, and thermally induced refolding, were systematically tested to optimize sensitivity and selectivity. The …

Genetic Property Rhetoric And The Public Domain, Dave Fagundes

Texas A&M Journal of Property Law

Among the many themes that illuminate Jorge Contreras’s The Genome Defense, this Essay focuses on property. It considers the rhetorical role played by property in convincing both courts and the public that patenting genetic data was legally and ethically objectionable. This Essay begins by locating property—and public property—as a major theme in the dialogue surrounding the Myriad litigation. It then situates this rhetorical move in the context of property-talk generally, highlighting the distinctiveness of leveraging public rather than private ownership as a means of accessing moral intuitions. Finally, this Essay reflects on what the use of public property rhetoric …

Functional Analysis Of Ligand Binding Domain 2 Of Outer Surface Protein C Of Borreliella Burgdorferi, Gavin Z. Chambers

Theses and Dissertations

Lyme disease, caused by the spirochete Borreliella burgdorferi, is the most common vector-borne infection in North America. A critical determinant of early mammalian infection is outer surface protein C (OspC), a highly variable lipoprotein that is indispensable for spirochete survival but whose precise function remains unresolved. OspC has been proposed to mediate diverse roles, including complement evasion, adhesion, tissue dissemination, and antiphagocytic activity, supporting the view that it is a multifunctional virulence factor. Structural studies have identified two putative ligand-binding domains (LBD1 and LBD2), with LBD2 forming a solvent-exposed crown at the membrane-distal end of the OspC dimer.

To …

Enhancing The Mechanical Properties Of Sla 3d-Printed Bio-Resin From Linseed Oil Using Cellulose And Lignin Biopolymers, Chukwunonso A. Ikedionu

College of Graduate Studies: Theses & Dissertations

Abstract

This research explores the development of bio resin from linseed oil by the process of epoxidation and acrylation to produce acrylated epoxidized linseed oil (AELO). Lignin and cellulose biopolymers were added as reinforcement at varying weight fractions of 1 wt.%, 2.5 wt.%, and 5 wt.%, and their mechanical performance was compared against pure AELO. The formulations were printed using an Elegoo SLA 3D printer to create standardized test specimens like tensile bars, compression cylinders, and hardness blocks, which were all prepared according to the ASTM testing standard.

The results indicate that reinforcement effects depend on both filler type and …

Influence Of Unmanned Aerial Vehicle (Uav) Payloads And Flight Speeds On Pesticide Droplet Deposition: Indoor Testing Rig Study, Siti Amni Ismail, Azmi Yahya, Ahmad Suhaizi Mat Su, Anas Mohd Mustafah, Norhayu Asib

ASEAN Journal on Science and Technology for Development

This study aims to determine droplet deposition resulting from dynamic change in payloads and flight speeds of Unmanned Aerial Vehicle (UAV) during operation. The study was conducted at Universiti Putra Malaysia, Serdang, Malaysia. The Advansia A1 hexacopter was first set to fly at combinations of 3 payloads (3.3, 6.6, and 9.9 kg) with 3 flight speeds (3, 5 and 7 m/s) to collect individual rotor RPM, which will later be used to set the rotors’ speed during indoor testing experiment. During the experiment, actual pesticide (Alosa EC) was used at 0.5% concentration in the spray deposition test. The droplet deposition …

Ribomoe: An Application Of Mixture Of Experts On Artificial Riboswitch Classification, Hainian Audrey Long

Master's Projects

Urban water sewage is a potential health concern due to its possibility to spread contagious RNA viruses such as Coxsackievirus B3. However, detection of viral particles remains challenging because of low viral concentrations in wastewater and high mutation rates of the RNA virus. To address this, this study proposes a novel viral detection method using synthetic riboswitches that bind to the target virus and trigger a reporter gene, amplifying the detection signals. To support the design of effective riboswitches, we present a machine learning model for classifying riboswitch performance, integrating RNA sequence data with secondary structural features. This model used …

Every Publication Needs An Author Verified Tl;Dr Statement, Sarah Oerther, Daniel B. Oerther

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

No abstract provided.

Investigating The Role Of Prostasin In Angiogenesis: A Chorioallantoic Membrane Model Approach, Zaima Aline

Honors Undergraduate Theses

Prostasin, also known as PRSS8 or CAP1, is a glycosylphosphatidylinositol (GPI)-anchored extracellular serine protease involved in various physiological and pathophysiological processes. Prostasin expression is observed during key phases of trophoblast invasion and placental development, critical steps in embryo implantation in Rhesus monkeys. This suggests a role for prostasin in extracellular matrix remodeling and tissue invasion, both essential for a healthy pregnancy. Moreover, prostasin appears vital for development as studies in mice show that a germline knockout of the gene leads to significant placental defects and eventual embryonic death, highlighting its importance in vascular and structural development within the placenta.

Building …

การวิเคราะห์เมตาโบโลมิกส์ในน้ำนมเหลืองและน้ำนมจากแม่สุกรที่ได้รับอาหารเสริมซินไบโอติก, เมธาพร เจือจันทร์

Chulalongkorn University Theses and Dissertations (Chula ETD)

ปัจจุบันมีการใช้สารเสริมอาหารสัตว์ในกลุ่มจุลินทรีย์โพรไบโอติกและซินไบโอติกเพื่อทดแทนการใช้สารปฏิชีวนะและลดปัญหาการเกิดเชื้อดื้อยาในอุตสาหกรรมการผลิตสุกร ซึ่งสารเสริมชีวนะดังกล่าวส่งผลในเชิงการส่งเสริมสุขภาพของแม่สุกร เพิ่มประสิทธิภาพในการสังเคราะห์องค์ประกอบและสารภูมิคุ้มกันในน้ำนม รวมทั้งช่วยลดอัตราการเกิดโรคท้องเสียในลูกสุกร อย่างไรก็ตาม การศึกษาข้อมูลสารชีวโมเลกุลขนาดเล็กหรือสารเมตาบอไลต์ในน้ำนมจากแม่สุกรที่ได้รับอาหารเสริมซินไบโอติกยังมีอยู่อย่างจำกัด ดังนั้น งานวิจัยนี้จึงมีวัตถุประสงค์เพื่อวิเคราะห์และเปรียบเทียบข้อมูลสารชีวโมเลกุลในน้ำนมจากแม่สุกรที่ได้รับอาหารที่มีการเสริมซินไบโอติก โดยพิจารณาร่วมกับอิทธิพลของระยะการให้นมและอิทธิพลจากลำดับครอกที่แตกต่างกันของแม่สุกร ผลการวิจัยพบว่าสามารถระบุชนิดของสารเมตาบอไลต์และกรดไขมันในตัวอย่างน้ำนมสุกรได้ทั้งหมด 47 และ 23 สาร โดยใช้เทคนิค 1H-NMR และ GC-FID ตามลำดับ ผลการวิเคราะห์ทางสถิติพหุตัวแปรแสดงให้เห็นว่าอิทธิพลจากระยะการให้นมส่งผลต่อการเปลี่ยนแปลงข้อมูลสารชีวโมเลกุลในน้ำนมสุกรอย่างชัดเจน เมื่อพิจารณาตัวอย่างน้ำนมที่เก็บในระยะเดียวกัน พบว่าอิทธิพลของการได้รับอาหารเสริมซินไบโอติกส่งผลต่อความแตกต่างของข้อมูลสาร เมตาบอไลต์ในตัวอย่างน้ำนมเหลืองและน้ำนมในวันที่ 10 เท่านั้น แต่อิทธิพลดังกล่าวส่งผลต่อการเปลี่ยนแปลงข้อมูลกรดไขมันตลอดทุกช่วงระยะการให้นม โดยสามารถใช้การเปลี่ยนแปลงปริมาณสัมพัทธ์ของ adenine, hypoxanthine, taurine, linolenic acid, linoleic acid และ lauric acid เป็นตัวบ่งชี้ทางชีวภาพเพื่อระบุความแตกต่างของน้ำนมจากแม่สุกรที่ได้รับอาหารเสริมซินไบโอติกได้ ทั้งนี้อิทธิพลจากลำดับครอกของแม่สุกรไม่ส่งผลต่อความแตกต่างของข้อมูลสารเมตาบอไลต์ แต่ส่งผลต่อความแตกต่างของข้อมูลกรดไขมันอย่างชัดเจนตลอดทุกช่วงระยะการให้นม โดยเฉพาะในแม่สุกรกลุ่มที่ได้รับอาหารเสริมซินไบโอติก ผลการวิจัยนี้แสดงให้เห็นถึงการประยุกต์ใช้เทคโนโลยีเมตาโบโลมิกส์ในการศึกษาการเปลี่ยนแปลงข้อมูลสารชีวโมเลกุลในน้ำนมสุกร และระบุชนิดของตัวบ่งชี้ทางชีวภาพต่ออิทธิพลของการได้รับอาหารเสริมซินไบโอติกได้อย่างมีประสิทธิภาพ