Biotechnology Commons^™

Stepwise Regression Analysis To Assess Various Factors Affecting On Fresh Sperm Quality In Ouled Djellal Ram, Yamina Belkhiri, Souheyla Benbia, Farida Bouzebda-Afri, Zoubir Bouzebda, Ramzi Lamraoui

The Thai Journal of Veterinary Medicine

The aim of this study was to evaluate the effect of various factors on fresh semen quality in Ouled Djellal rams. Specifically, we examined the impact of semen collection method, season, ram age, testosterone levels, body weight , and testicular weight. Twelve mature Ouled Djellal rams were used in the trial. Six rams were trained for semen collection using an artificial vagina (AV), while semen from the other six rams was collected using an electroejaculator (EE) biweekly throughout the year. Stepwise regression analysis showed that 62.50% of the variation in sperm volume, 48.60% of the variation in mass motility, 62.70% …

Novel Nanoformulation Of Piperlongumine Attenuates Pancreatic Tumor Desmoplasia And Modulates Tumor Immune Responses, Vivek K. Kashyap, Reinaldo Mandes, Neeraj Chauhan, Daniel Zubieta, Mohammed Sikander, Eswara N.H.K Ghali, Bilal B. Hafeez, Murali M. Yallapu, Subhash C. Chauhan

Research Symposium

Pancreatic cancer (PanCa) is characterized by a lack of early diagnosis, poor response to available therapeutic modalities, and chemoresistance. Gemcitabine (GEM) is currently considered the most effective therapy for PanCa; however, it shows only a marginal survival benefit of 6 months. This poor drug response has been attributed to desmoplasia, causes suboptimal drug delivery, alters tumor microenvironment (TME), which includes tumor surrounding blood vessels, fibroblasts, immune cells, extracellular matrix, and other signaling molecules and induces chemo-resistance in tumors. To overcome these existing issues associated with chemotherapy, the identification and development of novel therapeutic modalities are a pressing need. Piperlongumine (PL) …

Thermal Carbonization Of Biomass Wood Dust And Algae Wastes Via Microwave-Assisted H3Po4: Desirability Function And Statistical Optimization For Methylene Blue Dye Removal, Raja Razuan Raja Derisa, Hazierul F. Awang, Muhammad Hakim Azman, Intan Nur Hidayah Abdul Rashid, Ahmad Hapiz, Ruihong Wu, Abdallah Reghioua, Zaher Mundher Yaseen, Lee D. Wilson

AUIQ Complementary Biological System

This research utilized a carbonization procedure via microwave irradiation assisted by H₃PO₄ to generate a cost-effective adsorbent (CWDAG) from wood dust (WD) and algal (AG) biomass. The resulting CWDAG adsorbent was characterized for its methylene blue (MB) dye adsorption properties. The activation process employs 800 W microwave radiation for 15 min under a nitrogen gas (99.99%) atmosphere. Multiple techniques were employed to study the physicochemical properties of CWDAG, such as FTIR, XRD, FSEM-EDX, pH_pzc, and BET. Box-Behnken design (BBD) was employed to optimize the three important parameters of adsorption, as follows: A: CWDAG dosage …

Biological Infections Associated With Wearing Cosmetic Contact Lenses: Risks, Pathogens, And Preventative Measures: Mini Review, Lina M. Shaker, Wan Nor Roslam Wan Isahak

AUIQ Complementary Biological System

Cosmetic contact lenses are increasingly favored worldwide for their ability to enhance or alter aesthetic appearance. However, their use carries a higher risk of ocular infections, particularly when proper hygiene practices are neglected. This narrative review explores the biological and clinical aspects of infections associated with cosmetic lens usage, with a focus on pathogens such as Pseudomonas aeruginosa, Staphylococcus aureus (S. aureus), and Acanthamoeba. Key factors influencing infection susceptibility include microbial adhesion, biofilm formation, reduced oxygen permeability, and the effects of cosmetic pigments. The review further evaluates the role of lens material properties, including advancements in antimicrobial materials, …

Methylene Blue Dye Removal From Aqueous Solution Using Activated Carbon Prepared From Corn Cob Stem: Kinetics, Isotherms And Mechanism Studies, Gopi Narasimha Murthy, Uttam Kumar Sahu

AUIQ Complementary Biological System

In this study, a waste-to-wealth work has been proposed where activated carbon was synthesized from waste corn cob stems and used for methylene blue (MB) dye removal. Corn cob stems activated carbon (CCSAC) had been prepared in microwave technique using H₃PO₄ as an activating agent where the impregnation ratio of corn cob stems to H₃PO₄ was 1:4. Then CCSAC characterized by FTIR, BET, XRD, SEM, EDS, and Raman spectroscopy. The CCSAC showed the mesoporous with a good surface area of 54.61 m²/g. Around 150 mg adsorbent dose, 120 min contact time, pH …

Assessing The Biological Impacts And Health Risks Of Uv Radiation In The Context Of Sustainable Development Goals: Review, Ruaa H. Abbas, Lina M. Shaker

AUIQ Complementary Biological System

Ultraviolet (UV) radiation, while essential for certain biological processes, presents significant risks to human health and ecosystems. Increased UV exposure, driven by ozone depletion and climate change, heightens threats such as DNA damage, skin cancer, immune suppression, and eye disorders, directly aligning with Sustainable Development Goal (SDG) 3: Good Health and Well-being. Additionally, UV radiation disrupts marine and terrestrial ecosystems, impacting biodiversity and productivity, relevant to SDGs 14 and 15. This review evaluates the health and environmental impacts of UV radiation and highlights recent advancements in UV monitoring technology. Key findings reveal substantial evidence linking UV exposure to accelerated skin …

Biocomposite Adsorbent Of Grafted Chitosan-Benzaldehyde/Lactobacillus Casei Bacteria For Removal Of Acid Red 88 Dye: Box-Benken Design Optimization And Mechanism Approach, Hasan M. Aghaa, Salis A. Musa, Ahmad Hapiz, Ruihong Wu, Khansaa Al-Essa, Ali Mohammed Saleh, Abdallah Reghioua

AUIQ Complementary Biological System

Herein, biohybrid biosorbent material was produced from grafting chitosan benzaldehyde/Lactobacillus casei (CHS-BZ/LAC) for the removal of anionic dye acid red 88 (AR88). CHS-BZ/LAC was fabricated using a hydrothermal process under the condition of 100 °C in an oven for 6 h. The optimization of AR88 dye adsorption by CHS-BZ/LAC was conducted by employing Box Bahnken design (BBD). The code and value of parameters used in this study were CHS-BZ/LAC dosage (A: 0.02-0.1 g/L), dye solution pH (B: 4-10), and contact time (C: 10-40 min). The BBD design shows that the highest removal of …

Using Network Pharmacology To Systematically Deduce The Molecular Mechanism Of The Selective Perinucleolar Compartment Inhibitor Ml246, Bhuvnesh P. Sharma, Himanshu N. Singh, Bhupesh Singh, Deepak Parashar, Kuldeep K. Roy, Murali M. Yallapu, Subhash C. Chauhan, Vivek K. Kashyap

Research Symposium

Metarrestin (ML246) is an orally bioavailable synthetic molecule that selectively disrupts the perinucleolar compartment (PNC) structure, and it has showed promise in preclinical studies for metastatic cancer therapy. However, the precise molecular mechanism of ML246 remains poorly understood. We investigated the topological and protein interaction network (PIN) analyses of ML246 to determine the molecular mechanism of ML246. To determine the regulatory effect of ML246 on the rewired PIN of ML246, an intercom was constructed using 25 oncogenic key proteins. These proteins were selected using the reverse pharmacophore matching approach (based on a fit score > 0.502) using the STRING database and …

First-In-Class Peptide Molecules Targeting The Mien1 Cancer Signaling Pathway, For Which No Inhibitors Have Been Identified To Date., Amit Tripathi, Jamboor K. Vishwanatha

Research Symposium

Background: Migration and Invasion Enhancer 1 (MIEN1) is a critical oncogene that drives cancer cell migration, invasion, and epithelial-to-mesenchymal transition (EMT) in various cancers. MIEN1 overexpression is linked to aggressive tumor progression and poor prognosis. Despite its established role in cancer biology, targeted therapeutic options remain unexplored. This study aimed to identify and characterize first-in-class therapeutic molecules targeting the MIEN1 signaling pathway to inhibit cancer progression.

Methods: To develop novel bio-active peptides, we leveraged conserved immunoreceptor tyrosine-based activation motif (ITAM) and prenylation motifs within the MIEN1 protein sequence. Using rational peptide design, we identified and synthesized bioactive peptides, namely LA3IK …

Influence Of Nitrogen And Phosphorus Rates On The Growth Of Water Hyacinth (Pontederia Crassipes Mart.), Mary Joy M. Abit, Rene Franchesca A. Pilar, Analiza Henedina M. Ramirez

The Philippine Agricultural Scientist

Research has been conducted globally to investigate the growth and colonization patterns of Pontederia crassipes Mart. This research aimed to examine the impact of varying rates of nitrogen (N) and phosphorus (P) on the growth of P. crassipes and determine the nutrient levels that would result in the greatest growth response. P. crassipes plants were placed in plastic containers filled with 5 L of distilled water. Nitrogen and phosphorus were applied separately at varying concentrations. Specifically, N was added at 0, 10, 20, 30, 40, or 50 mg L^-1 and P at 0, 1, 3, 5, 7, or 9 …

Morphoanatomy And Phenoplasticity Of Cardamine Hupingshanensis (Brassicaceae) Under Cadmium Stress, Yafen Han, Florfe M. Acma, Maria Melanie M. Guiang, Chris Rey M. Lituanas, Fulgent P. Coritico, Victor B. Amoroso

The Philippine Agricultural Scientist

Using a soil culture method, this study investigated the morphoanatomical responses and phenotypic plasticity of Cardamine hupingshanensis (Brassicaceae) under different cadmium (Cd) concentrations. Root morphological indices initially increased at low Cd levels but decreased as Cd concentrations rose, exhibiting a low-promoting and high-inhibiting effect. Leaf morphology and biomass peaked at 5 or 10 mg kg^-1 Cd before decreasing at higher concentrations but were still significantly higher than the control (P < 0.05). Anatomical structure analysis revealed no significant difference at 5 mg kg^-1 Cd; however, at ≥ 100 mg kg^-1 Cd, epidermal and spongy tissue thickness differed significantly from the control. Correlation analysis indicated a significant positive correlation between …

Insulin Receptor Responsiveness Governs Tgfβ-Induced Hepatic Stellate Cell Activation: Insulin Resistance Instigates Liver Fibrosis, Wang-Hsin Lee, Evelyn A. Bates, Zachary A. Kipp, Sally Pauss, Genesee J. Martinez, Cheavar A. Blair, Terry D. Hinds Jr.

Markey Cancer Center Faculty Publications

The insulin receptor (INSR) has been shown to be hyperactive in hepatic stellate cells (HSCs) in humans and rodents with liver fibrosis. To explore HSC cellular mechanisms that INSR regulates during pro-fibrotic stimulation, we used CRISPR-Cas9 technology. We knocked out a portion of the INSR gene in human LX2 HSC cells (INSRe5- 8 KO) that regulates insulin responsiveness but not the insulin-like growth factor (IGF) or transforming growth factor-β (TGFβ) signaling. The INSRe5- 8 KO HSCs had significantly higher cell growth, BrdU incorporation, and lower TP53 expression that suppresses growth, and they also exhibited increased migration compared to the Scramble …

A Double-Sided Hybrid Lc Series/Parallel Compensation Topology For An Efficient Magnetic Resonance Wireless Power Transfer Systems, Talha Irshad, Malik Nauman, Pg Emeroylariffion Abas

ASEAN Journal on Science and Technology for Development

Conventional plug-in charging faces numerous challenges due to radiation hazards, inconvenience, etc. To mitigate the challenges conventional charging methods face, wireless power transfer (WPT) systems emerged as an efficient method for wireless energy transmission across various applications such as electric vehicles, biomedical implants, smartphones, and network sensors. A robust WPT system needs to perform exceptionally well under varying conditions, however, the WPT performance is reduced due to the leakage inductance between the coils. To address this challenge, compensation networks are often employed to minimize the losses and enhance the overall performance of the WPT system. This paper focuses on designing …

Let’S Be Cool About It: Anticipating Proper, Whole-Human Cryopreservation And The Necessary Oversight For Adequate Consumer Protection, Peter Lyon

Minnesota Journal of Law, Science & Technology

No abstract provided.

Agile Project Management And The Future Of R&D, Aditi Vinayak Nalavade

Harrisburg University Dissertations and Theses

The use of Agile methodologies in research and development (R&D) sectors is changing the project management landscape by profoundly altering approaches to innovation and execution. This research will delve into the benefits and challenges of implementing Agile in an R&D setting, with a particular focus on the increasing significance of AI. The research's central theme offers a comprehensive analysis of how Agile approaches are shaping the future of R&D. Agile's iterative process, with its high degree of adaptability, flexibility, and iterative methods, is particularly beneficial in R&D, where reshaping research teams' approaches to creativity and problem-solving even in the most …

Identification Of Novel Genetic Biomarkers Linked To The Prodromal Stage Of Parkinson’S Disease (Pd) Patients, Marwa Tawfik Badawy

Theses and Dissertations

Background and Objective: The early diagnosis of neurodegenerative diseases, such as Parkinson's disease (PD), is particularly challenging because symptoms appear only after significant neuronal damage has already occurred. This study is utilizing variant call format (VCF) analysis to identify genetic variants and novel genes that could serve as early prognostic markers for prodromal PD. Materials and Methods: Data were sourced from the Parkinson's Progression Markers Initiative (PPMI), focusing on prodromal patients with gVCF data from the 2021 cohort. The study included 304 participants, comprising 100 healthy controls, 146 individuals with prodromal genetic indicators, 21 individuals with prodromal hyposmia, and 37 …

Identification Of Novel Argonaute Proteins Using A Metagenomic Mining Approach, Lobna Abdallah Ghonaim

Theses and Dissertations

Gene editing is one of the most promising tools in science. It enables precise modifications of an organism's genetic material. Metagenomics is considered a powerful tool that unlocks the broad genetic potential found in uncultured microbial communities. Exploring the genetic diversity of uncultured microbial communities helps identify novel functional proteins with unique properties and make the best use of these diverse microbial ecosystems.

We developed and employed a metagenomic-based approach to mine more than 1000 metagenomes for prokaryotic argonaute proteins (pAgos), a potential gene editing machinery encoded in bacterial and archaeal genomes. Our workflow involved strict quality control, sequence assembly, …

Strength And Durability Characteristics Of Mortar Containing Fine Glass Powders As Partial Substitute Of Cement, Deepa Paul, Bindhu K.R, G. M. Sadiqul Islam, Bindhu K. R.

ASEAN Journal on Science and Technology for Development

A recent trend in construction involves the application of high-strength mortar which consumes a high quantity of cement. The present study aims at producing a sustainable mortar utilising waste glass powders (GP) as a partial substitute for cement in mortar. GP grains having different particle sizes in equal proportions were carried out in the mortar mix for improved packing. After realising the cementing properties of GP particles from the microanalyses like micron-size, silica-rich, reactive, and thermal resistance, mechanical and durability aspects of the mortar composite with manufactured sand as a fine aggregate were examined. The mechanical properties of the mortar …

The Role Of Gsk-3Α Inhibition In Attenuating Lps-Induced Inflammation In Raw 264.7 Cells, Oluwaseyi Oyewole

Theses and Dissertations

Glycogen synthase kinase-3 (GSK-3), an enzyme that modulates glucose metabolism, has been recognized as an essential target in major inflammatory diseases based on its great specificity in substrate recognition. GSK-3β has been connected to cancer, obesity, liver diseases, and neurological diseases. Nonetheless, the other GSK-3 isoform, GSK-3α, is much less studied. Previous studies from our laboratory showed that inhibiting GSK-3α potentially confers an anti-inflammatory response when tested on microglia. However, little has been documented regarding the exact mechanism by which GSK-3α exerts its effect. One of the potential mechanisms involving its anti-inflammatory effect could be partially attributed to activating the …

Immuno-Bioinformatics Study Of Secondary Metabolites From Watercress Nasturtium Officinale As Immunostimulant In Whiteleg Shrimp Litopenaeus Vannamei By Targeting Peroxinectin And Scavenger Receptor Class B, Qurrota A’Yunin, Muhaimin Rifa’I, Maftuch Maftuch, Yoga Dwi Jatmiko, Muhammad Hermawan Widyananda, Triyanto Triyanto, Muhammad Tegar Handrianto

Karbala International Journal of Modern Science

The viral disease affects the whiteleg shrimp (Litopenaeus vannamei) and causes losses. Immunostimulants are recognized as a more environmentally friendly approach to disease management and antimicrobial properties of aquatic organisms. Watercress (Nasturtium officinale) is an aquatic plant species that contains antioxidant properties that can protect the organism from disease and boost the immune system. Shrimp rely heavily on innate immunity to combat infectious agents due to their absence of adaptive immunity. Peroxinectin and scavenger receptor class B (SRB) play essential roles in the shrimp defense system. This research employs a bioinformatic to assess the immunostimulatory potential …