Metabolic Comparison Of Wild-Type And Transgenic Synechocystis Pcc 6803 Cyanobacteria,
2017
Purdue University
Metabolic Comparison Of Wild-Type And Transgenic Synechocystis Pcc 6803 Cyanobacteria, Ian A. Mcluckey, John A. Morgan, Joel Yu King Hing
The Summer Undergraduate Research Fellowship (SURF) Symposium
The Calvin-Benson (CBB) cycle is an essential part of nature. This phenomenon allows carbon molecules in carbon dioxide from the atmosphere to be converted into useful energy in the form of sugars. Cyanobacteria are single-celled organisms capable of utilizing energy from sunlight to drive this cycle and are also readily engineered. In hopes of improving this cycle, we compared a wild-type version of the Synechocystis PCC6803 cyanobacteria to an engineered version overexpressing the enzyme FBA (fructose-biphosphate aldolase), called 70 glpX, to deduce how the overexpressing strain is able to be more photosynthetically efficient. To do this, comparative metabolomics were done …
The Response Of Schwann Cells To Weak Dc Electric Fields,
2017
Purdue University
The Response Of Schwann Cells To Weak Dc Electric Fields, Alexander T. Lai, Jianming Li
The Summer Undergraduate Research Fellowship (SURF) Symposium
Schwann cells are glial cells that serve the vital role of supporting neurons in the peripheral nervous system. While their primary function is to provide insulation (myelin) for axons, they also help regenerate injured axons by digesting severed axons and providing scaffolding to guide the regeneration process. This specific role of Schwann cells makes them highly important cellular targets following nerve injury. Although some efforts have been made to encourage Schwann cell migration after nerve damage, the use of electric fields to control cell responses remain unexplored; therefore, this experiment serves to characterize the behavior of Schwann cells to weak …
Fluorescent Protein Biosensor For Use In Parkinson's Research,
2017
Purdue University
Fluorescent Protein Biosensor For Use In Parkinson's Research, Piper R. Miller, Keelan Trull, Mathew Tantama
The Summer Undergraduate Research Fellowship (SURF) Symposium
Purinergic signaling is a type of extracellular communication that occurs between cells, mediated by adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine. In Parkinson’s Disease, purinergic signaling is disrupted, which contributes to neurodegeneration. In order to monitor this change in cell-to-cell signaling, there is a need for the development of a fluorescent protein (FP) biosensor to study the changes in the concentration of the signaling molecule ATP and its decomposition bioproduct ADP. This summer a genetically encoded ADP sensor that measures changes in ADP concentration was developed. This sensor utilizes Forster Resonance Energy Transfer (FRET) which is a sensing technique …
Gas-Based Perfusion Fmri Cerebrovascular Reactivity Maps And Time Of Arrival Maps,
2017
Purdue University
Gas-Based Perfusion Fmri Cerebrovascular Reactivity Maps And Time Of Arrival Maps, Jinxia Yao, Yunjie Tong, Matthew Robert Derdak
The Summer Undergraduate Research Fellowship (SURF) Symposium
Perfusion MRI is a promising tool used in assessing stroke, brain tumors, and patients with neurodegenerative diseases. However, common perfusion MRI techniques have several disadvantages, such as using exogenous contrast agents, low signal-to-noise ratio and long acquisition time. In this study, we used a computer-controlled gas delivery system RespirAct TM. This system provides a reliable carbon dioxide based vasoactive stimulus. This stimulus will immediately generate large and reliable functional magnetic resonance imaging (fMRI) signals, which can be used for perfusion imaging. Each subject was required to have three sequential magnetic resonance imaging (MRI) scans, which are structural scan, resting state …
Identification And Metabolite Profiling Of Chemical
Activators Of Lipid Accumulation In Green Algae,
2017
University of Nebraska-Lincoln
Identification And Metabolite Profiling Of Chemical Activators Of Lipid Accumulation In Green Algae, Nishikant Wase, Boqiang Tu, James Allen, Paul N. Black, Concetta Dirusso
Department of Biochemistry: Faculty Publications
Microalgae are proposed as feedstock organisms useful for producing biofuels and coproducts. However, several limitations must be overcome before algae-based production is economically feasible. Among these is the ability to induce lipid accumulation and storage without affecting biomass yield. To overcome this barrier, a chemical genetics approach was employed in which 43,783 compounds were screened against Chlamydomonas reinhardtii, and 243 compounds were identified that increase triacylglyceride (TAG) accumulation without terminating growth. Identified compounds were classified by structural similarity, and 15 were selected for secondary analyses addressing impacts on growth fitness, photosynthetic pigments, and total cellular protein and starch concentrations. TAG …
Using Silkworms As A Host To Spin Spider Silk-Like Fibers,
2017
Utah State University
Using Silkworms As A Host To Spin Spider Silk-Like Fibers, Xiaoli Zhang
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Using silkworms as the potential host to spin spider silk-like fibers is an area of intense research world-wide. The conventional methods used to create transgenic silkworms hosting spider silk-like gene limits the incorporation of spider silk-like protein and do not improve the mechanical performance of the composite silkworm/spider silk fibers. In this dissertation, synthetic spider ampullate genes were incorporated into the precise site of the fibroin heavy chain or light chain using the latest genome editing technology CRISPR/cas9 guided non-homologous end joining as opposed to conventional random integration using transposon-based piggyBac system. These protocols, with extensive applicability to other silkworm …
Decision Making Processes For A Patient With Cardiac Pacemaker Admitted To The Accident And Emergency Undergoing Magnetic Resonance Imaging – A Case Study,
2017
Universiti Brunei Darussalam
Decision Making Processes For A Patient With Cardiac Pacemaker Admitted To The Accident And Emergency Undergoing Magnetic Resonance Imaging – A Case Study, Raditya F. P.
ASEAN Journal on Science and Technology for Development
This case study aims to apply the processes of decision-making for magnetic resonance imaging (MRI) as a diagnostic examination for a patient with cardiac pacemaker admitted to a private hospital in the capital of Indonesia. It also aims to examine and evaluate the case of a 53-year-old mother who had a heart abnormality that has a pacemaker for one year and was diagnosed to do an MRI examination because of a suspected tumor on the wrist area.The three basic models of decision-making — the descriptive, prescriptive and normative — were applied. The prescriptive used the information processing theory, while the …
Decision Making Processes For A Pregnant Woman Admitted To The Accident And Emergency Department Requiring Emergency Diagnostic X-Ray – A Case Study,
2017
Universiti Brunei Darussalam
Decision Making Processes For A Pregnant Woman Admitted To The Accident And Emergency Department Requiring Emergency Diagnostic X-Ray – A Case Study, Ismanto S.
ASEAN Journal on Science and Technology for Development
The objective of this study is to apply the decision-making processes for a pregnant woman who was involved in a motor vehicle accident and admitted to a private middle-class hospital in the capital of Indonesia requiring radiologic X-ray examination. It also aims to examine and evaluate the patient who was in her 20th week of gestation in order to provide her with the best emergency care, diagnostic investigations and treatments.The descriptive, normative and prescriptive models of decision-making are demonstrated. The descriptive model used intuition, while the normative model used decision trees as decision options and lastly the prescriptive decision used …
Utilization Of Waste From Natural Rubber Glove Manufacturing Line,
2017
Universiti Brunei Darussalam
Utilization Of Waste From Natural Rubber Glove Manufacturing Line, Devaraj V., Nur F. I., Dayang A. I. H., Nor H. K., Zairossani M. N.
ASEAN Journal on Science and Technology for Development
Malaysia is the largest producer and exporter of examination and surgical gloves in the world and currently faced with mitigating large amounts of waste generated during the glove production process. This waste is mainly generated from glove dipping tanks and is referred as dipping tank coagulum (DTC). It is considered as scheduled waste which requires mandatory disposal by incineration, in compliance to the Scheduled Waste Regulations set by the Department of Environment. Work described in this study showed, DTC samples with a polymer content of >40%, both ash and calcium carbonate content of <10% and curatives <2% (Sulphur, antioxidants, accelerators and ZnO) when blended with virgin rubbers (SMR 10 and SMR 20) were found to be suitable for manufacturing value-added rubber products. DTC samples with polymer contents of <40% and lower in curativescould still be considered for recycling, by adding higher portions of virgin rubber for manufacturing products like shoe soles, carpet underlay and thermoplastic elastomer products. Glove manufactures should ideally set up on-site DTC processing facilities at their factory premises equipped with crepers as well as space to ‘air dry’ the creped DTC samples. Creped samples could be sent to the Malaysian Rubber Board (MRB) for chemical analyses. Factory owners could also present the analytical results from MRB to the recyclers to obtain a good premium for their processed DTC samples to be used as raw materials.
Phase Manifestation And Formation Of Nanoemulsions Composed Of Imidazolium-Based Ionic Liquid, Tween 80/Span 80 And Labrafac Lipophile Wl 1349,
2017
Universiti Brunei Darussalam
Phase Manifestation And Formation Of Nanoemulsions Composed Of Imidazolium-Based Ionic Liquid, Tween 80/Span 80 And Labrafac Lipophile Wl 1349, Ng S. H., Woi P. M., Eng C. C.
ASEAN Journal on Science and Technology for Development
onic liquids (ILs) can enhance topical and transdermal delivery, as well as increase the solubility of sparingly soluble drugs. In the present work, pseudo-ternary phase diagrams of emulsions were composed of a mixture of non-ionic surfactants, polyoxyethylene sorbitan monooleate (Tween 80®) and sorbitan monooleate (Span 80®) in weight fraction: 1:1, 1:2, 2:1 and 2:3, LabrafacTM Lipophile WL 1349 as an oil phase and 1-hexyl-3-methylimidazolium chloride [(HMIM) (Cl)] as a continuous phase. Emulsion formulations were selected with 10% surfactants from the pseudo-ternary phase diagrams and further prepared at 298.2 ± 0.1 K. Acoustic emulsificationmethod was used to prepare nanoemulsions that were …
A Phenomenological Study On The Quality Of Life Among Patients With Osteoarthritis Admitted For Rehabilitative Physiotherapy In A Private Hospital In Kuala Lumpur,
2017
Universiti Brunei Darussalam
A Phenomenological Study On The Quality Of Life Among Patients With Osteoarthritis Admitted For Rehabilitative Physiotherapy In A Private Hospital In Kuala Lumpur, Dioso R. (Iii) P., Tanggaya R.
ASEAN Journal on Science and Technology for Development
The aim of this study is to explore patients’ experiences and how their rehabilitation and physiotherapy at a private hospital in Kuala Lumpur, Malaysia has affected their quality of life (QOL) due to osteoarthritis (OA). In addition, this study also aims to explore patients’ perspectives of the rehabilitation and physiotherapy for their OA before and after which affects moreover their QOL. The seven domains of the QOL were the physiological, social, financial,environmental, psychological, level of independence and spiritual – aimed to guide the theme of the interview sessions. A qualitative design was used. Of the 50 patients purposively enrolled only …
Editorial Board,
2017
Universiti Brunei Darussalam
Editorial Board, Ajstd Editorialboard
ASEAN Journal on Science and Technology for Development
No abstract provided.
Physiological Evidence For Isopotential Tunneling In The Electron Transport Chain Of Methane-Producing Archaea,
2017
University of Nebraska - Lincoln
Physiological Evidence For Isopotential Tunneling In The Electron Transport Chain Of Methane-Producing Archaea, Nikolas Duszenko, Nicole R. Buan
Department of Biochemistry: Faculty Publications
Many, but not all, organisms use quinones to conserve energy in their electron transport chains. Fermentative bacteria and methane-producing archaea (methanogens) do not produce quinones but have devised other ways to generate ATP. Methanophenazine (MPh) is a unique membrane electron carrier found in Methanosarcina species that plays the same role as quinones in the electron transport chain. To extend the analogy between quinones and MPh, we compared the MPh pool sizes between two well-studied Methanosarcina species, Methanosarcina acetivorans C2A and Methanosarcina barkeri Fusaro, to the quinone pool size in the bacterium Escherichia coli. We found the quantity of MPh per …
Rational Drug Design Directed At Blocking The Initial Signaling Events In Lipopolysaccharide-Induced Sepsis.,
2017
University of Missouri - St. Louis
Rational Drug Design Directed At Blocking The Initial Signaling Events In Lipopolysaccharide-Induced Sepsis., Christopher A. Tipton
Theses
Systemic Inflammatory Response Syndrome (SIRS) is classified as an immune system response to an infectious state. If left untreated, SIRS leads to sepsis, septic shock, end-organ dysfunction, and death. As a patient progresses through these stages, associations of acute respiratory distress, disseminated intravascular coagulation, and acute renal failure persist, resulting in millions of deaths annually. Lipopolysaccharide (LPS), a bacterial endotoxin, is released into the blood stream, triggering SIRS. LPS is found in the outer cell-wall of Gram-negative bacteria and is responsible for initiation of a devastating cytokine storm. One of the regions of LPS, lipid A, is a polyacylated glucosamine …
Methanolysis Of Crude Jatropha Oil Using Heterogeneous Catalyst From The Seashells And Eggshells As Green Biodiesel,
2017
Universiti Brunei Darussalam
Methanolysis Of Crude Jatropha Oil Using Heterogeneous Catalyst From The Seashells And Eggshells As Green Biodiesel, Reddy A. N. R., Ahmed A. S., Islam M. D., Hamdan S.
ASEAN Journal on Science and Technology for Development
In this work, heterogeneous calcium oxide catalysts gleaned from Polymedosa expansa and eggshell were investigated for the transesterification of crude jatropha oil with methanol, to access their prospective performance in biodiesel production as an alternative green energy resource. The best yield of biodiesel achieved was 96% in 1 h for Step 1 using 0.01:1 ratio of acid catalyst to oil and 0.6:1 ratio of alcohol to oil ratio, together with 2 h of Step 2 using 0.02:1 ratio with base catalyst CaO, derived from P. expansa, to oil ratio and 5:1 ratio of alcohol to oil. The properties of jatropha …
Structures, Energies, And Bonding Analysis Of Monoaurated Complexes With N-Heterocyclic Carbene And Analogues,
2017
Universiti Brunei Darussalam
Structures, Energies, And Bonding Analysis Of Monoaurated Complexes With N-Heterocyclic Carbene And Analogues, Nguyen T.A.N., Huynh T.P.L., Vo T.X.P., Tran T.H., Tran D.S., Dang T.H., Duong T.Q.
ASEAN Journal on Science and Technology for Development
In this work, we computationally investigated from quantum chemical calculations (DFT) at the BP86 level with the various basis sets def2-SVP, def2-TZVPP, and TZ2P+, chemical bonding issues of the recently described carbene-analogues gold(I) complexes AuCl-NHEMe (Au1-NHE) with E = C – Pb. The optimized structures and the metal-ligand bond dissociation energy (BDE) were calculated, and the nature of the E→Au bond was studied with charge and energy decomposition methods. The equilibrium structures of the system showed that there were major differences in the bonded orientation from the ligands NHC-NHPb to gold(I) complex between the lighter and the heavier homologues. The …
Decision-Making Processes For A Do-Not-Resuscitate Poisoned Pediatric Patient Admitted To The Department Of Emergency And Medical Services — A Case Study,
2017
Universiti Brunei Darussalam
Decision-Making Processes For A Do-Not-Resuscitate Poisoned Pediatric Patient Admitted To The Department Of Emergency And Medical Services — A Case Study, Dioso R. (Iii) P.
ASEAN Journal on Science and Technology for Development
A team of healthcare professionals in the Department of Emergency and Medical Services demonstrated decision-making processes for a poisoned pediatric patient with a Do-not-resuscitate (DNR) order. At the same time, clinical guidelines for poison resuscitations were critically applied. Lastly, the case examined and evaluated emergency care, management, treatment and diagnostic laboratory tests for pediatric poisoned patients. Ideas on pertinent literatures of decision-making models, clinical guidelines on poison resuscitation and DNR policies were already known by the majority. In addition, ideas on the benefits such as becoming enlightened on how to make sound judgments in an emergency situation, particularly addressing issues …
Periphytic Diatoms In The Polluted Linggi (Sensu Stricto) And Kundor Rivers, Negeri Sembilan, Malaysia,
2017
Universiti Brunei Darussalam
Periphytic Diatoms In The Polluted Linggi (Sensu Stricto) And Kundor Rivers, Negeri Sembilan, Malaysia, Khan I.S.A. Nather
ASEAN Journal on Science and Technology for Development
The investigation in this paper aimed to describe periphytic diatom assemblage, species composition and distribution in tropical polluted Linggi (sensu stricto or s.s.) and Kundor rivers in Negeri Sembilan, Malaysia. Regardless of ecological and environmental conditions, diatoms were the numerically dominant flora among periphytic algae collected monthly over a period of 13 months at nine stations in the Linggi (s.s.) and Kundor rivers. The freshwater periphytic diatom samples were collected mainly using artificial substrates and supplemented with natural substrates. The periphytic diatoms thus collected from both natural and artificial substrates comprised 86 taxa (82 pennate and 4 centric forms) belonging …
Short Communication: Sulphur Levels And Fuel Quality In Peninsular Malaysia,
2017
Universiti Brunei Darussalam
Short Communication: Sulphur Levels And Fuel Quality In Peninsular Malaysia, Ramalingam M., Fuad A. Ahmad
ASEAN Journal on Science and Technology for Development
This paper presents the investigation on sulphur levels in fuels collected from different petrol stations in Peninsular Malaysia. Diesel and gasoline samples were analysed for sulphur using X-ray fluorescence spectrometry technique. In general, diesel concentrations of sulphur were only slightly lower than the Deprtment of Environment’s current sulphur limit of 500 p.p.m. across 2010–2014 (the range was 289–698 p.p.m., 374–410 p.p.m., 319–436 p.p.m., 447–605 p.p.m. and 300–477 p.p.m. for 2010, 2011, 2012, 2013 and 2014, respectively). Sulphur concentrations were relatively low in gasoline samples: the mean figures were 95, 77, 96, 74, 240 and 125 for 2009, 2010, 2011, 2012, …
eraack
A Rapid Colorimetric Peptide Nucleic Acid Loop-Mediated Isothermal Amplification Assay For The Detection Of The Idh1 Mutation In Glioblastoma, Edward J. Raack
All NMU Master's Theses
The IDH1 mutation is an important diagnostic and prognostic biomarker used to characterize glioblastoma (GBM). Patients harboring the IDH1 mutation have improved overall survival following maximal resection. Knowledge of the IDH1 mutation status allows the surgeon to modify the surgical plan; however, no existing molecular test can provide this information intraoperatively. We designed a novel colorimetric peptide nucleic acid loop-mediated isothermal amplification (PNA-LAMP) method that rapidly detects the IDH1 R132H mutation in GBM. PNA-LAMP amplifies target DNA under isothermal conditions with high specificity and speed. The PNA prevents amplification of wild-type IDH1 DNA, while allowing amplification of the R132H variant …
