A Brief Elevation Of Serum Amyloid A Is Sufficient To Increase Atherosclerosis, 2014 University of Kentucky
A Brief Elevation Of Serum Amyloid A Is Sufficient To Increase Atherosclerosis, Joel C. Thompson
Theses and Dissertations--Pharmacology and Nutritional Sciences
Cardiovascular disease is now the leading cause of death worldwide. Serum amyloid A (SAA), a positive acute phase reactant, along with C-reactive protein is used clinically as a marker of cardiovascular disease risk. However, recent data has shed light on a possible causal role of SAA in the development of atherosclerosis, the most pervasive form of cardiovascular disease. Several inflammatory diseases such as diabetes and obesity are known to confer increased risk of developing cardiovascular disease. Individuals with these diseases all have modest but persistent elevation of SAA. To determine if SAA caused the development of atherosclerosis, apoe-/-chow …
Receptor Of Advanced Glycation Endproducts (Rage) Is Positively Correlated With Tumor Necrosis Factor-Α In Adolescents With Obesity, 2014 Virginia Commonwealth University
Receptor Of Advanced Glycation Endproducts (Rage) Is Positively Correlated With Tumor Necrosis Factor-Α In Adolescents With Obesity, Tasnim Rahman, Daniel H. Conrad, Anshu Gupta
Undergraduate Research Posters
Introduction: Obesity in childhood is associated with an increased prevalence of diabetes and other traditional cardiometabolic risk factors, suggesting an epidemic of premature cardiovascular disease among today’s youth. Glycotoxins, known as advanced glycationend products (AGE’s), activating via the membrane-bound receptors (mRAGE), have been implicated in the pathophysiology of inflammation, (increased tumor necrosis factor-α [TNF-α ]), insulin resistance and vascular dysfunction in adults, but the role of RAGE in the early stages of metabolic disorders is unknown. In this study, we assessed relationship of cardiometabolicrisk factors, mRNA expression of TNF-α and RAGE in peripheral monocytes in adolescents with obesity.
Methods: Thirty …
The Prevalence And Effect Of Wolbachia Infection On The Brown Widow Spider (Latrodectus Geometricus), 2014 Georgia Southern University
The Prevalence And Effect Of Wolbachia Infection On The Brown Widow Spider (Latrodectus Geometricus), Brittany Dane' Arrington
Electronic Theses and Dissertations
Wolbachia was recently identified in the Brown Widow Spider (Latrodectus geometricus). This symbiont has not previously been found in this genus, therefore the consequences on the host’s population dynamics are unknown. The frequency of endosymbiont infection can provide insight into the population dynamics of the host as this bacteria is known to alter host life history. In my 1st chapter I determine the prevalence of Wolbachia pipentis infection in in the Brown Widow Spider (Latrodectus geometricus) from five locations in the Southeastern United States. Infection frequency differed significantly among sampling locations, ranging from 20% to …
The Impact Of Dams On Nitrogen Cycling In The Messalonskee Stream, 2014 Colby College
The Impact Of Dams On Nitrogen Cycling In The Messalonskee Stream, Rebecca K. Forgrave
Honors Theses
The Messalonskee Stream in central Maine has five hydroelectric dams on 16.6km. Each dam drastically changes the flow regime of the stream, dividing it into segments with different patterns of sediment settling and organic matter retention. I investigated how these disruptions impact nitrogen cycling, specifically nitrification rates above and below each dam. I expected higher nitrification rates above the dams, where levels of organic matter are higher, and lower rates below the dam where scouring removes organic matter and fine sediment from the streambed. I measured sediment nitrification rates with a nitrapyrin-inhibition assay and potential drivers of nitrification including sediment …
Observing The Unwatchable Through Acceleration Logging Of Animal Behavior, 2013 Western Kentucky University
Observing The Unwatchable Through Acceleration Logging Of Animal Behavior, Danielle D. Brown, Roland Kays, Martin Wikelski, Rory Wilson, A. Peter Klimley
Methodology and Animal Models in Research
Behavior is an important mechanism of evolution and it is paid for through energy expenditure. Nevertheless, field biologists can rarely observe animals for more than a fraction of their daily activities and attempts to quantify behavior for modeling ecological processes often exclude cryptic yet important behavioral events. Over the past few years, an explosion of research on remote monitoring of animal behavior using acceleration sensors has smashed the decades-old limits of observational studies. Animal-attached accelerometers measure the change in velocity of the body over time and can quantify fine-scale movements and body postures unlimited by visibility, observer bias, or the …
Investigation Of The Role Of Tor And Nutrient Restriction On Lifespan Of Arabidopsis Thaliana, 2013 Dominican University of California
Investigation Of The Role Of Tor And Nutrient Restriction On Lifespan Of Arabidopsis Thaliana, Debashree Banerjee
Dissertations, Masters Theses, Capstones, and Culminating Projects
The Target of Rapamycin (TOR) kinase is evolutionarily conserved and is a key regulator, controlling many cellular functions to promote survival and growth in all eukaryotes. Several model organisms have indicated a relationship between TOR signaling and life expectancy, such as yeast, fruit flies and mice. Abundant nutrient availability promotes rapid growth and development, whereas depletion of nutrients reduces the activity of the pathways that are involved in growth and nutrient processing. This reduction in activity increases life expectancy, which is supported by evolutionary theories. The confirmed presence of the TOR homolog (a major regulator of growth and cell proliferation) …
The Roles Of Phenotypic Plasticity And Genotypic Specialization In High Altitude Adaptation, 2013 University of Nebraska-Lincoln
The Roles Of Phenotypic Plasticity And Genotypic Specialization In High Altitude Adaptation, Danielle M. Tufts
School of Biological Sciences: Dissertations, Theses, and Student Research
In vertebrates living at high altitude, arterial hypoxemia may be ameliorated by reversible changes in the oxygen-carrying capacity of the blood (regulated by erythropoiesis) and/or changes in blood–oxygen affinity (regulated by allosteric effectors of hemoglobin function). These hematological traits often differ between taxa that are native to different elevational zones, but it is often unknown whether the observed physiological differences reflect fixed, genetically based differences or environmentally induced acclimatization responses (phenotypic plasticity). Here, we report measurements of hematological traits related to blood–O2 transport in populations of deer mice (Peromyscus maniculatus) that are native to high- and low-altitude …
Developmental And Molecular Functions Of Plakophilin-3, 2013 The University of Texas Graduate School of Biomedical Sciences at Houston
Developmental And Molecular Functions Of Plakophilin-3, William A. Munoz
Dissertations & Theses (Open Access)
Plakophilin-3, the less studied member of the plakophilin-catenin subfamily, and the larger catenin family, binds directly to desmosomal cadherin cytoplasmic domains and enhances desmosome formation and stability. In mammals, plakophilin-3 is expressed at the highest levels in desmosome-enriched tissues such as epithelia, with the knock-out in mice producing corresponding reductions in ectodermal integrity. In tissue, cellular and intracellular contexts where plakophilin-3 is not at the desmosomal plaque, little is known about its functions in the cytoplasm or nucleus, where it also localizes.
My work employed embryos of the amphibian, Xenopus laevis, to examine plakophilin-3’s developmental roles. I first evaluated …
Stellaris Fishing 20131125mon Part 1 Of 2, 2013 M.D. Anderson Cancer Center
Stellaris Fishing 20131125mon Part 1 Of 2, George Mcnamara
George McNamara
Stellaris FISH dataset using three FISH probe sets. Slides courtesy of Biosearch Technologies,
https://www.biosearchtech.com/store/product.aspx?catid=224,318,324
see http://stellarisfish.smugmug.com/ for online gallery by Biosearch.
This experiment was to evaluate the crosstalk between the Biosearch fluorophores:
Quasar 570
CAL Fluor Red 610 (CFR 610)
Quasar 670
DAPI (DNA counterstain)
Autofluorescence (green, but sometimes showing up in other channels).
and our lab's Leica DMI6000 fluorescence microscope with Leica filter sets:
DAPI
GFP (L5)
Cy3 (N3)
Texas Red (TxRed2)
Cy5 (Y5)
I also acquired green channel and red channel with exciter filters in our ASI excitation wheel:
GFP + 492 exciter
Texas Red (TxRed2) + 572 …
Cytotoxicity In The Age Of Nano: The Role Of Fourth Period Transition Metal Oxide Nanoparticle Physicochemical Properties, 2013 Middle Tennessee State University
Cytotoxicity In The Age Of Nano: The Role Of Fourth Period Transition Metal Oxide Nanoparticle Physicochemical Properties, Charles C. Chusuei, Chi-Heng Wu, Shravan Mallavarapu, Fang Yao Stephen Hou, Chen-Ming Hsu, Jeffrey G. Winiarz, Robert S. Aronstam, Yue-Wern Huang
Clinical Lab Sciences Faculty Research and Publications
A clear understanding of physicochemical factors governing nanoparticle toxicity is still in its infancy. We used a systematic approach to delineate physicochemical properties of nanoparticles that govern cytotoxicity. The cytotoxicity of fourth period metal oxide nanoparticles (NPs): TiO2, Cr2O3, Mn2O3, Fe2O3, NiO, CuO, and ZnO increases with the atomic number of the transition metal oxide. This trend was not cell-type specific, as observed in non-transformed human lung cells (BEAS-2B) and human bronchoalveolar carcinoma-derived cells (A549). Addition of NPs to the cell culture medium did not significantly …
Iron-Regulated Cyanobacterial Predominance And Siderophore Production In Oligotrophic Freshwater Lakes, 2013 The University of Western Ontario
Iron-Regulated Cyanobacterial Predominance And Siderophore Production In Oligotrophic Freshwater Lakes, Ryan J. Sorichetti
Electronic Thesis and Dissertation Repository
The frequency and intensity of cyanobacterial blooms (cyanoblooms) is increasing globally. Contrary to existing phosphorus (P) and nitrogen (N) paradigms describing cyanobloom proliferation in eutrophic (nutrient-rich) freshwater lakes, many of the recent cyanobloom reports pertain to oligotrophic (nutrient-poor) freshwater lakes with no prior history of cyanobloom occurrence. There exists a critical research need to re-visit existing conceptual models, identify regulating factors currently unaccounted for and improve our ability to effectively detect and measure cyanobacterial toxins (cyanotoxins) in lakes. Iron (Fe) is required in nearly all pathways of cyanobacterial macronutrient use, though its direct role in regulating cyanobacterial biomass is not …
The Utilization Of Chiral Ion Mobility Spectrometry For The Detection Of Enantiomeric Mixtures And Thermally Labile Compounds, 2013 Florida International University
The Utilization Of Chiral Ion Mobility Spectrometry For The Detection Of Enantiomeric Mixtures And Thermally Labile Compounds, Howard K. Holness
FIU Electronic Theses and Dissertations
This dissertation utilized electrospray ion mobility mass spectrometry (ESI-IMS-MS) to develop methods necessary for the separation of chiral compounds of forensic interest. The compounds separated included ephedrines and pseudoephedrines, that occur as impurities in confiscated amphetamine type substances (ATS) in an effort to determine the origin of these substances. The ESI-IMS-MS technique proved to be faster and more cost effective than traditional chromatographic methods currently used to conduct chiral separations such as gas and liquid chromatography. Both mass spectrometric and computational analysis revealed the separation mechanism of these chiral interactions allowing for further development to separate other chiral compounds by …
Incorporating New Technologies Into Toxicity Testing And Risk Assessment: Moving From 21st Century Vision To A Data-Driven Framework, 2013 The Hamner Institutes for Health Sciences
Incorporating New Technologies Into Toxicity Testing And Risk Assessment: Moving From 21st Century Vision To A Data-Driven Framework, Russell S. Thomas, Martin A. Philbert, Scott Auerbach, Barbara A. Wetmore, Michael J. Devito, Ila Cote, J. Craig Rowlands, Maurice Whelan, Sean M. Hays, Melvin E. Andersen, M.E. (Bette) Meek, Lawrence W. Reiter, Jason C. Lambert, Harvey J. Clewell Iii, Martin L. Stephens, Q. Jay Zhao, Scott C. Wesselkamper, Lynn Flowers, Edward W. Carney, Tim P. Pastoor, Dan D. Petersen, Carole L. Yauk, Andy Nong
in Vitro Research Models Collection
Based on existing data and previous work, a series of studies is proposed as a basis toward a pragmatic early step in transforming toxicity testing. These studies were assembled into a data-driven framework that invokes successive tiers of testing with margin of exposure (MOE) as the primary metric. The first tier of the framework integrates data from high-throughput in vitro assays, in vitro-to-in vivo extrapolation (IVIVE) pharmacokinetic modeling, and exposure modeling. The in vitro assays are used to separate chemicals based on their relative selectivity in interacting with biological targets and identify the concentration at which these interactions …
Facile Synthesis, Characterization, And Antimicrobial Activity Of Cellulose-Chitosan-Hydroxyapatite Composite Material: A Potential Material For Bone Tissue Engineering, 2013 Marquette University
Facile Synthesis, Characterization, And Antimicrobial Activity Of Cellulose-Chitosan-Hydroxyapatite Composite Material: A Potential Material For Bone Tissue Engineering, Tamutsiwa M. Mututuvari, April Harkins, Chieu D. Tran
Clinical Lab Sciences Faculty Research and Publications
Hydroxyapatite (HAp) is often used as a bone-implant material because it is biocompatible and osteoconductive. However, HAp possesses poor rheological properties and it is inactive against disease-causing microbes. To improve these properties, we developed a green method to synthesize multifunctional composites containing: (1) cellulose (CEL) to impart mechanical strength; (2) chitosan (CS) to induce antibacterial activity thereby maintaining a microbe-free wound site; and (3) HAp. In this method, CS and CEL were co-dissolved in an ionic liquid (IL) and then regenerated from water. HAp was subsequently formed in situ by alternately soaking [CEL+CS] composites in aqueous solutions of CaCl2 …
Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, 2013 Jain Irrigation Systems Ltd., Jalgaon, Maharashtra, India
Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati
Rajesh Pati
A standard uniform protocol of regeneration is the prime and foremost prerequisite for not only improve the productivity, the production from the existing area but also development of transgenics in mango for various traits. Polyembryonic cultivars are good rootstock because of moderate canopy, tolerant to draught, salt, environmental stress (wind, high temperature, air pollution), fungal disease (anthracnose, powdery mildew, gummosis) and hoppers.
Genetic Transformation Of Bael (Aegle Marmelos Corr.), 2013 Jain Irrigation Systems Ltd., Jalgaon, Maharashtra, India
Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati
Rajesh Pati
Aegle marmelos Corr. is better known for its medicinal values rather than its edible quality. Every part of the plant viz. leaves, fruits, roots, and the bark are used for treating various diseases related to heart, stomach, bacterial, fungal, viral, worms, fertility, fever and cosmetics. Marmelosin is one of the major compounds (coumarin), used to treat various diseases. Till date, there are no varieties developed for commercial orchards of Bael and hence, regarded as one among the underutilized fruits of India. Therefore, there is an urgent need for breeding new and cultivable varieties in Aegle marmelos. But the problem for …
Micropropagation In Fruit Crops, 2013 Jain Irrigation Systems Ltd., Jalgaon, Maharashtra, India
Micropropagation In Fruit Crops, Rajesh Pati
Rajesh Pati
Micropropagation is a proven means of producing millions of identical plants under a controlled and aseptic condition, independent of seasonal constraints. It not only provides economy of time and space but also gives greater output and allows further augmentation of elite disease free propagules. India is homeland of many important fruit crops such as Indian gooseberry (Emblica officinalis Gaertn), bael (Aegle marmelos Corr.), Guava (, Psidium guajava), jamun or black plum (Syzygium cuminii L. Skeels.), Mango (Mangifera indica) and Papaya (Carica papaya).
In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., 2013 Jain Irrigation Systems Ltd., Jalgaon, Maharashtra, India
In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati
Rajesh Pati
Bael (Aegle marmelos Corr.) is an important medicinal fruit tree. The fruit pulp contains marmelosin, which is a laxative, diuretic, is being used in many patented drugs in India. Micropropagation technology can be gainfully employed in mass multiplication of improved bael varieties. We have developed micropropagation protocol of bael through shoot bud culture. It was imperative to test genetic fidelity of micropropagated plants using molecular markers (RAPD, DAMD and ISSR).
Identification & Quantitation Of Bacteria In Emergency Eyewash Bottles Used In Science Laboratories, 2013 Kent State University - Kent Campus
Identification & Quantitation Of Bacteria In Emergency Eyewash Bottles Used In Science Laboratories, Christopher J. Woolverton, Jerry Lillestedt
Christopher J. Woolverton
No abstract provided.
Use Of Veterinary Records To Teach Laboratory Thinking Skills In Biology, 2013 Kent State University - Kent Campus
Use Of Veterinary Records To Teach Laboratory Thinking Skills In Biology, Christopher Woolverton
Christopher J. Woolverton
No abstract provided.