Biotechnology Commons^™

Nlrp10 Enhances Cd4+ T-Cell-Mediated Ifnγ Response Via Regulation Of Dendritic Cell-Derived Il-12 Release, Maurizio Vacca, Julia Böhme, Lisa Zambetti, Hanif Javanmard Khameneh, Bhairav S. Paleja, Federica Laudisi, Adrian W. S. Ho, Kurt Neo, Keith Weng Kit Leong, Mardiana Marzuki, Bernett Lee, Michael Poidinger, Laura Santambrogio, Liana Tsenova, Francesca Zolezzi, Gennaro De Libero, Amit Singhal, Alessandra Mortellaro

Publications and Research

NLRP10 is a nucleotide-binding oligomerization domain-like receptor that functions as an intracellular pattern recognition receptor for microbial products. Here, we generated a Nlrp10−/− mouse to delineate the role of NLRP10 in the host immune response and found that Nlrp10−/− dendritic cells (DCs) elicited sub-optimal IFNγ production by antigenspecific CD4+ T cells compared to wild-type (WT) DCs. In response to T-cell encounter, CD40 ligation or Toll-like receptor 9 stimulation, Nlrp10−/− DCs produced low levels of IL-12, due to a substantial decrease in NF-κB activation. Defective IL-12 production was also evident in vivo and affected IFNγ production by CD4+ T cells. Upon …

Optimizing Sperm Collection Procedures In Zebrafish, Madelyn B. Wasden, Rachel L. Roberts, April Delaurier

Journal of the South Carolina Academy of Science

Zebrafish are a highly-valued model organism used for developmental biology research. Zebrafish can be used for genetic manipulation and hence, many mutant and transgenic lines exist. It is impractical to maintain lines of adult zebrafish, due to resource constraints and the need to continuously produce new generations. Therefore, a practical way to preserve zebrafish lines is to freeze sperm and retrieve lines using in vitro fertilization of fresh eggs. Most existing in vitro protocols used by research labs have a wide variety of fertilization rates (ranging from 0% to >90%). Due to this variability, lines may be at risk of …

System Enginering - Is It Relevant To Brunei, Ginige A, Knight J. A

ASEAN Journal on Science and Technology for Development

System Enginering - Is IT Relevant to Brunei

Microbial Halogenation With Specific Reference To Chiloroperoxidase, Smith D.C, Bult A. T, Holt G, Christy C, Lilly M. D, Rice C

ASEAN Journal on Science and Technology for Development

Microbial Halogenation With Specific Reference to Chiloroperoxidase

Biogas Plants With Plastic Film Gasholder, Tentscher W

ASEAN Journal on Science and Technology for Development

Biogas Plants With Plastic Film Gasholder

Optical Properties And Aging Characteristics Of Blackchrme Surface On Different Textures Of Aluminium Substrates, Trakulcoo Kanchana, Niemsorn Saowanee, Pitaksenpanich Patcharee

ASEAN Journal on Science and Technology for Development

Optical Properties and Aging Characteristics of Blackchrme Surface on Different Textures of Aluminium Substrates

Employment Situation In Manufacturing Industry In Asean: A Few Issues, Chandra M, Satpute Chhaya

ASEAN Journal on Science and Technology for Development

Employment Situation in Manufacturing Industry in Asean: A Few Issues

Energy Model Of Grain Drying System, Soponronnarit Somchart

ASEAN Journal on Science and Technology for Development

Energy Model of Grain Drying System

Introduction Of Cosmid Phu 52 Into Hup Azospirillum Sp. Bss 8 By Transconjugation And Transformation, Mamaril Juanita C, Ko Elizabeth P, Trinidad Lorele C.

ASEAN Journal on Science and Technology for Development

Introduction of Cosmid pHU 52 Into HUP Azospirillum SP. BSs 8 BY Transconjugation and Transformation

An Operational System For Mapping Global Solar Radiation From Gms Satellite Data, Sorapipatana Chumnong, Exell R.H.B

ASEAN Journal on Science and Technology for Development

An Operational System For Mapping Global Solar Radiation From GMS Satellite Data

Book Review, Man In The Mangroves: The Socio Economic Situation Of Human Settlements In Mangrove Forest, Soegiarto Aprilani

ASEAN Journal on Science and Technology for Development

Book Review, Man in the Mangroves: The Socio Economic Situation of Human Settlements in Mangrove Forest

Pv Power System Component Characterization And System Simulation, Lasnier F, Ang T.G, Wang Y. J, Hemasuk T

ASEAN Journal on Science and Technology for Development

PV Power System Component Characterization and System Simulation

Crossflow Ultrafiltration For Removing Direct-15 Dye From Wastewater Of Textile Industry, Ahmad A.L., Puasa S.W., Abiding S.

ASEAN Journal on Science and Technology for Development

Ultrafiltration membrane was used to treat the effluent from textile industries. Crossflow ultrafiltration using GN polymeric membrane was used to remove the dye from textile effluent. A synthetic textile effluent of Direct-15 dye was used. The study focused through the effect of feed concentration, transmembrane pressure and solution’s pH on the permeate flux and percentage of dye removal were investigated. Dye concentration had significant effects on flux values. Under the fixed pressures and pH, the flux decreased while the dye rejection increased with increasing feed concentration. Transmembrane pressure also had significant effect on flux values. Under the fixed feed concentration …

Mitochondrial Reactive Oxygen Species In Lipotoxic Hearts Induces Post-Translational Modifications Of Akap121, Drp1 And Opa1 That Promote Mitochondrial Fission, Kensuke Tsushima, Heiko Bugger, Adam R. Wende, Jamie Soto, Gregory A. Jenson, Austin R. Tor, Rose Mcglauflin, Helena C. Kenny, Yuan Zhang, Rhonda Souvenir, Xiao X. Hu, Crystal L. Sloan, Renata O. Pereira, Vitor A. Lira, Kenneth W. Spitzer, Terry L. Sharp, Kooresh I. Shoghi, Genevieve C. Sparagna, Eva A. Rog-Zielinska, Peter Kohl, Oleh Khalimonchuk, Jean E. Schaffer, E. Dale Abel

Department of Biochemistry: Faculty Publications

Rationale: Cardiac lipotoxicity, characterized by increased uptake, oxidation and accumulation of lipid intermediates, contributes to cardiac dysfunction in obesity and diabetes. However, mechanisms linking lipid overload and mitochondrial dysfunction are incompletely understood.

Objective: To elucidate the mechanisms for mitochondrial adaptations to lipid overload in postnatal hearts in vivo.

Methods and Results: Using a transgenic mouse model of cardiac lipotoxicity overexpressing long-chain acyl-CoA synthetase 1 in cardiomyocytes, we show that modestly increased myocardial fatty acid uptake leads to mitochondrial structural remodeling with significant reduction in minimum diameter. This is associated with increased palmitoyl-carnitine oxidation and increased reactive oxygen species (ROS) generation …

Status And Potential Of Co - Generation In Thai Rice Mills, Chantramonklasri Nit, Wibulswas Prida

ASEAN Journal on Science and Technology for Development

Status and Potential of Co - Generation in Thai Rice Mills

Effects Of Habitat Fragmentation On Genetic Diversity In Cycas Balansae (Cycadaceae), Tam Nguyen Minh, Trang Nguyen T. Phuong, Giang Vu T. Ha, Triest L. Triest

ASEAN Journal on Science and Technology for Development

Habitat fragmentation is a serious threat to species survival. In Vietnam, Cycas balansae has been considered as threatened species because of the reduction and fragmentation of its habitats and over-exploitation. We assessed genetic variability and the pattern of population structure among six populations sampled in four provinces: Hoa Binh, Ha Nam, Ninh Binh and Quang Ninh. Polyacrylamide gel electrophoresis was performed on leaf tissues from 152 individuals representing 6 populations of C. balansae. Six of twelve enzyme systems were used to estimate genetic diversity at population and species levels. Eleven loci were examined. The allozyme data showed high levels of …

Technology Research And Development For Environmental Management - With Specific Reference To Malaysia, Yeoh B. Ghin, Idrus Ahmad Zaharudin, Ong K.S

ASEAN Journal on Science and Technology for Development

Technology Research and Development for Environmental Management - With Specific Reference to Malaysia

Research And Development Of Solar Thermal Energy In Thailand, Wibulswas Prida

ASEAN Journal on Science and Technology for Development

Research and Development of Solar Thermal Energy in Thailand

A Study Of Organelles Associated With Vitellogenesis In Oncomelania Hupensis Quadrasi, A Molluscan Host Of Schictosoma Japonicum, Claveria F.G, Etges F.J

ASEAN Journal on Science and Technology for Development

A Study of Organelles Associated with Vitellogenesis in Oncomelania Hupensis Quadrasi, A Molluscan Host of Schictosoma Japonicum

A Low Cost Hpib Compatible Interface To A Thermochuck For Computerised C-V Measurement System, Kwok C.K, Ling C.H, Kanhere R, Ah L.K

ASEAN Journal on Science and Technology for Development

A Low Cost HPIB Compatible Interface to a Thermochuck for Computerised C-V Measurement System