Biotechnology Commons^™

The Relationship Between Kras And The Endoplasmic Reticulum-Mitochondrial Calcium Flux In Isogenic Colorectal Cancer Cell Lines, Emma Anderson, Abigail Solitro

Liberty University Research Week

Undergraduate

Theoretical Proposal

B25: Generation And Characterization Of Sars-Cov-2 Spike Protein-Pseudotyped Lentivirus Particles For Use In Neutralizing Antibody Titer Testing Of Human Serum, Luis Sanchez

Annual Research Symposium

No abstract provided.

Pengaruh Air Kelapa Sebagai Sitokinin Organik Dan Sukrosa Terhadap Pertumbuhan Protocorm Anggrek (Phalaenopsis Hybrid Mp 253 X F1 3363 (M)) In Vitro, Muhammad Isyraq, Lia Amalia, Iis Aisyah

Jurnal Kultivasi

Sitokinin dan sukrosa dibutuhkan untuk meregenerasi protocorm anggrek. Air kelapa diketahui memiliki kandungan sitokinin sehingga berpotensi dijadikan sebagai sitokinin organik. Percobaan ini bertujuan untuk mempelajari pengaruh konsentrasi air kelapa sebagai sitokinin organik dan sukrosa terhadap pertumbuhan protocorm anggrek. Percobaan ini dilaksanakan di Laboratorium Bioteknologi Tanaman, Fakultas Pertanian Universitas Winaya Mukti, Kecamatan Tanjungsari Kabupaten Sumedang. Percobaan dimulai pada bulan Mei sampai dengan bulan Agustus 2019. Rancangan percobaan yang digunakan adalah rancangan acak kelompok dengan dua faktor dan diulang dua kali. Faktor pertama adalah konsentrasi air kelapa, terdiri dari lima taraf yaitu 0 mL L-1, 50 mL L-1, 100 mL L-1, air …

Pertumbuhan Tunas Kunyit Tinggi Kurkumin Pada Berbagai Jenis Sitokinin Dan Auksin Secara In Vitro, Erni Suminar, Denny Sobardini Sobarna, Syariful Mubarok, Sulistyaningsih, Ade Setiawan

Jurnal Kultivasi

Kunyit merupakan salah satu tanaman yang banyak dimanfaatkan oleh masyarakat sebagai bahan baku obat tradisional, bumbu dapur, serta zat pewarna alami, sehingga kebutuhannya mengalami peningkatan setiap tahunnya. Penyediaan bibit yang memiliki produktivitas tinggi dalam jumlah banyak dapat dilakukan dengan metode kultur in vitro, namun perlu optimasi media yang sesuai. Penelitian ini bertujuan untuk mencari komposisi zat pengatur tumbuh yang dapat memberikan pertumbuhan planlet yang lebih vigor. Rancangan percobaan yang digunakan yaitu Rancangan Acak Lengkap. Perlakuan terdiri dari penggunaan sitokinin dan auksin pada eksplan kunyit yang diulang tiga kali. Media Murashige and Skoog (MS) digunakan sebagai media dasar dengan perlakuan kombinasi …

Isoprene Production From Municipal Wastewater Biosolids By Engineered Archaeon Methanosarcina Acetivorans, Sean Carr, Jared Aldridge, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Wastewater biosolids are a promising feedstock for production of value-added renewable chemicals. Methane-producing archaea (methanogens) are already used to produce renewable biogas via the anaerobic treatment of wastewater. The ability of methanogens to efficiently convert dissolved organic carbon into methane makes them an appealing potential platform for biorefining using metabolic engineering. We have engineered a strain of the methanogen Methanosarcina acetivorans to produce the volatile hemiterpene isoprene in addition to methane. The engineered strain was adapted to grow in municipal wastewater through cultivation in a synthetic wastewater medium. When introduced to municipal wastewater the engineered methanogens were able to compete …

Isoprene Production From Municipal Wastewater Biosolids By Engineered Archaeon Methanosarcina Acetivorans, Sean Carr, Jared Aldridge, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Wastewater biosolids are a promising feedstock for production of value-added renewable chemicals. Methane-producing archaea (methanogens) are already used to produce renewable biogas via the anaerobic treatment of wastewater. The ability of methanogens to efficiently convert dissolved organic carbon into methane makes them an appealing potential platform for biorefining using metabolic engineering. We have engineered a strain of the methanogen Methanosarcina acetivorans to produce the volatile hemiterpene isoprene in addition to methane. The engineered strain was adapted to grow in municipal wastewater through cultivation in a synthetic wastewater medium. When introduced to municipal wastewater the engineered methanogens were able to compete …

Development Of Miriplatin-Loaded Nanoparticles Against Non-Small Cell Lung Cancer, Zhongyue Yuan, Zizhao Xu, Xin Guo

Graduate and Professional Student Research Showcase

Lung cancer claims the highest mortality and the second-most new cases among all oncological diseases. NSCLC accounts for approximately 85% of all newly diagnosed lung cancers. Although platinum-based drugs are standard first-line chemotherapy for stage IIIB/IV NSCLC, accumulating reports have shown the failure of conventional platinum-based regimens due to drug resistance. Miriplatin is a lipophilic anti-cancer drug that has been approved in Japan for transcatheter arterial chemoembolization treatment of hepatocellular carcinoma. Lipid-based nanoparticles such as liposomes, micelles, and solid lipid nanoparticles (SLNs) can encapsulate anti-cancer drugs to improve their water solubility and bioavailability.

Structural Basis For Clostridium Perfringens Enterotoxin Targeting Of Claudins At Tight Junctions In Mammalian Gut, Alex J. Vecchio, Sewwandi S. Rathnayake, Robert M. Stroud

Department of Biochemistry: Faculty Publications

The bacterium Clostridium perfringens causes severe, sometimes lethal gastrointestinal disorders in humans, including enteritis and enterotoxemia. Type F strains produce an enterotoxin (CpE) that causes the third most common foodborne illness in the United States. CpE induces gut breakdown by disrupting barriers at cell–cell contacts called tight junctions (TJs), which are formed and maintained by claudins. Targeted binding of CpE to specific claudins, encoded by its C-terminal domain (cCpE), loosens TJ barriers to trigger molecular leaks between cells. Cytotoxicity results from claudin-bound CpE complexes forming pores in cell membranes. In mammalian tissues, ∼24 claudins govern TJ barriers—but the basis for …

Implementation Of A Small-Scale Wind Turbine, Lewis University

Sustainability

No abstract provided.

Responses Of Soil Surface Greenhouse Gas Emissions To Nitrogen And Sulfur Fertilizer Rates To Brassica Carinata Grown As A Bio-Jet Fuel, Dwarika Bhattarai, Gandura O. Abagandura, Thandiwe Nleya, Sandeep Kumar

Agronomy, Horticulture and Plant Science Faculty Publications

Carinata (Brassica carinata A. Braun), a non-food oilseed crop and an alternative bio-jet fuel feedstock, has received attention for its potential as a low-input option for production in the semi-arid region of the Northern Great Plains of the United States. Research addressing the impacts of nitrogen (N) and sulfur (S) fertilizers on soils and greenhouse gas (GHG; CO₂, N₂O, and CH₄) emissions from carinata production are limited. Thus, objective of this study was to evaluate the impact of different rates of N and S fertilizers applied to carinata on soil properties and GHG …

Transcriptomic Analysis Of The Glycophytic Crop Ipomoea Aquatica Under Salinity Conditions Compared To Its Wild Halophyte Relative Ipomoea Pes-Caprae, Ruwan Saeed Aljneibi Husam Saeed Aljneibi

Theses

This study focuses on the glycophytic crop Ipomoea Aquatica (commonly known as water spinach) and its wild halophytic relative Ipomoea pes-caprae. I. Aquatica is a crop with economic value; however, it is unable to tolerate high levels of salinity. Whereas its relative Ipomoea pes-caprae is able to grow and thrive in the harsh environment of the UAE. The main aim of this study is to analyze the genetic differences underlying the variation in the two plants’ response to salinity and determine the genetic components that can be used to enhance I. Aquatica's tolerance to salinity. Accordingly, the plants were subjected …

Highlighting Membrane Protein Structure And Function: A Celebration Of The Protein Data Bank, Fei Li, Pascal F. Egea, Alex J. Vecchio, Ignacio Asial, Meghna Gupta, Joana Paulino, Ruchika Bajaj, Miles Sasha Dickinson, Shelagh Fergunson-Miller, Brian C. Monk, Robert M. Stroud

Department of Biochemistry: Faculty Publications

Biological membranes define the boundaries of cells and compartmentalize the chemical and physical processes required for life. Many biological processes are carried out by proteins embedded in or associated with such membranes. Determination of membrane protein (MP) structures at atomic or near-atomic resolution plays a vital role in elucidating their structural and functional impact in biology. This endeavor has determined 1198 unique MP structures as of early 2021. The value of these structures is expanded greatly by deposition of their three-dimensional (3D) coordinates into the Protein Data Bank (PDB) after the first atomic MP structure was elucidated in 1985. Since …

A Co-Opted Steroid Synthesis Gene, Maintained In Sorghum But Not Maize, Is Associated With A Divergence In Leaf Wax Chemistry, Lucas Busta, Elizabeth Schmitz, Dylan K. Kosma, James Schnable, Edgar B. Cahoon

Department of Biochemistry: Faculty Publications

Virtually all land plants are coated in a cuticle, a waxy polyester that prevents nonstomatal water loss and is important for heat and drought tolerance. Here, we describe a likely genetic basis for a divergence in cuticular wax chemistry between Sorghum bicolor, a drought tolerant crop widely cultivated in hot climates, and its close relative Zea mays (maize). Combining chemical analyses, heterologous expression, and comparative genomics, we reveal that: 1) sorghum and maize leaf waxes are similar at the juvenile stage but, after the juvenile-to-adult transition, sorghum leaf waxes are rich in triterpenoids that are absent from maize; 2) …

Comparative Expression Studies Of Fiber Related Genes In Cotton Spp., Muhammad Nadeem, Nadia Iqbal, Ummara Waheed, Zia Ullah Zia, Muqarrab Ali, Zulqurnain Khan

Journal of Bioresource Management

Cotton fibers are the seed trichomes that are developed around the seed and are used to make clothes and yarn for the textile industry. Expression profiling of cotton fiber genes is very important to estimate the differential gene expression level at different fiber developmental stages. Expression analysis of fiber developing genes are very important to enhance the fiber length of cotton. The expression profiling of three gene families in five stages (0, 5, 10, 15 and 20 DPA) of cotton fiber tissues was carried out through real-time PCR. Expression analysis revealed that transcripts of GA-20 Oxidase, XTH, …

Primer Payload System For Higher-Order Multiplex Lamp: Design And Development Of Unit Processes, Tochukwu Dubem Anyaduba

KGI Theses and Dissertations

Design and Development of Platforms for the Application of Loop-mediated Isothermal Nucleic Acid Amplification, LAMP, in the Diagnosis of Polymicrobial Diseases

Tochukwu Dubem Anyaduba, Travis Schlappi (PI)

For the past two decades, several isothermal nucleic acid amplification technologies have emerged. These are mostly in response to the need for robust molecular diagnostic tools amenable to point-of-care and limited-resource settings. Of these, loop-mediated isothermal amplification, LAMP, stands out as a highly specific and rapid alternative to the polymerase chain reaction, PCR. One of LAMP's significant characteristics involves using four essential and two loop (rate increasing) primers to recognize six to eight …

Nih Funding For Vaccine Readiness Before The Covid-19 Pandemic, Anthony E. Kiszewski, Ekaterina Galkina Cleary, Matthew J. Jackson, Fred D. Ledley

Natural & Applied Sciences Faculty Publications

Rapid development of vaccines for COVID-19 has relied on the application of existing vaccine technologies. This work examines the maturity of ten technologies employed in candidate vaccines (as of July 2020) and NIH funding for published research on these technologies from 2000–2019. These technologies vary from established platforms, which have been used successfully in approved products, to emerging technologies with no prior clinical validation. A robust body of published research on vaccine technologies was supported by 16,358 fiscal years of NIH funding totaling $17.2 billion from 2000–2019. During this period, NIH funding for published vaccine research against specific pandemic threats …

Structural Basis For Clostridium Perfringens Enterotoxin Targeting Of Claudins At Tight Junctions In Mammalian Gut, Alex J. Vecchio, Sewwandi S. Rathnayake, Robert M. Stroud

Department of Biochemistry: Faculty Publications

The bacterium Clostridium perfringens causes severe, sometimes lethal gastrointestinal disorders in humans, including enteritis and enterotoxemia. Type F strains produce an enterotoxin (CpE) that causes the third most common foodborne illness in the United States. CpE induces gut breakdown by disrupting barriers at cell–cell contacts called tight junctions (TJs), which are formed and maintained by claudins. Targeted binding of CpE to specific claudins, encoded by its C-terminal domain (cCpE), loosens TJ barriers to trigger molecular leaks between cells. Cytotoxicity results from claudin-bound CpE complexes forming pores in cell membranes. In mammalian tissues, 24 claudins govern TJ barriers—but the basis for …

Protease Oma1 Modulates Mitochondrial Bioenergetics And Ultrastructure Through Dynamic Association With Micos Complex, Martonio Ponte Viana, Roman M. Levytskyy, Ruchika Anand, Andreas S. Reichert, Oleh Khalimonchuk

Department of Biochemistry: Faculty Publications

Remodeling of mitochondrial ultrastructure is a process that is critical for organelle physiology and apoptosis. Although the key players in this process—mitochondrial contact site and cristae junction organizing system (MICOS) and Optic Atrophy 1 (OPA1)—have been characterized, the mechanisms behind its regulation remain incompletely defined. Here, we found that in addition to its role in mitochondrial division, metallopeptidase OMA1 is required for the maintenance of intermembrane connectivity through dynamic association with MICOS. This association is independent of OPA1, mediated via the MICOS subunit MIC60, and is important for stability of MICOS and the intermembrane contacts. The OMA1-MICOS relay is required …

Evaluation Of The Anticancer Potential Of Alchemilla Vulgaris Extract Against Human Neuroblastoma Cells, Yomna Moqidem

Theses and Dissertations

Neuroblastoma is one of the most challenging pediatric tumors that present heterogeneity in prognosis and survival despite intensive treatment protocols. Therefore, there is an urge to develop novel and more potent anticancer drugs. Medicinal plants have recently gained worldwide attention due to their variable activities, wide availability, and cost-efficiency. Accordingly, this study attempted to identify novel plant extracts that could show potential anticancer properties against neuroblastoma. In this study, Alchemilla vulgaris (A.vulgaris) ethyl acetate extract was prepared and evaluated for its anticancer potential against SH-SY5Y Human neuroblastoma cell line. The Phenolic content of the prepared extract was characterized …

Mining Selected Metagenomes/Metatranscriptomes For Biosynthetic Gene Clusters And Antimicrobial Resistance Genes, Ahmed Yamany

Theses and Dissertations

Antimicrobial resistance is one of the serious global challenges in the current century. The fact that resistance genes transfer between bacteria, coupled with the fact that the world is connected through complex dynamics. Studying microbial behavior and understanding the different factors coffering microbial resistance to a broad spectrum of the available drug classes, parallel with a comprehensive analysis of the natural microbial products as the primary source of the novel antibiotics, might shed some light on solutions for this problem. Microbial environments harbor a wide range of secondary metabolites (SM) with different functional groups. SMs are not directly involved in …