Analysis Of Symptom Expressions And Transmission Rates Caused By The Plant Pathogen Phytophthora Ramorum On Native Chaparral Plants From The Genus Arctostaphylos,
2021
Dominican University of California
Analysis Of Symptom Expressions And Transmission Rates Caused By The Plant Pathogen Phytophthora Ramorum On Native Chaparral Plants From The Genus Arctostaphylos, Bharati Gaonker
Natural Sciences and Mathematics | Biological Sciences Master's Theses
Phytophthora ramorum is the causal agent of Sudden Oak death (SOD), ramorum dieback and ramorum leaf blight which affect both forest environments and nurseries. This oomycete pathogen has had a huge economic impact on the nursery and lumber industry. Forests in California have experienced substantial mortality of oaks affecting the forest dynamics and diversity. Our research investigates four native species and two ornamental cultivars of plants, which belong to the genus Arctostaphylos (manzanita) and are considered to be new hosts for P. ramorum in the chaparral ecosystem of California. Symptom expression and transmission rates were analyzed on Arctostaphylos glauca, …
Multi-Strategy Engineering Greatly Enhances Provitamin
A Carotenoid Accumulation And Stability In Arabidopsis Seeds,
2021
Cornell University
Multi-Strategy Engineering Greatly Enhances Provitamin A Carotenoid Accumulation And Stability In Arabidopsis Seeds, Tianhu Sun, Qinlong Zhu, Ziqing Wei, Lauren A. Owens, Tara Fish, Hyojin Kim, Theodore W. Thannhauser, Edgar B. Cahoon, Li Li
Department of Biochemistry: Faculty Publications
Staple grains with low levels of provitamin A carotenoids contribute to the global prevalence of vitamin A deficiency and therefore are the main targets for provitamin A biofortification. However, carotenoid stability during both seed maturation and postharvest storage is a serious concern for the full benefits of carotenoid biofortified grains. In this study, we utilized Arabidopsis as a model to establish carotenoid biofortification strategies in seeds. We discovered that manipulation of carotenoid biosynthetic activity by seed-specific expression of Phytoene synthase (PSY) increases both provitamin A and total carotenoid levels but the increased carotenoids are prone to degradation during …
Pepblock Builder Vr – An Open-Source Tool For Gaming-Based Bio-Edutainment In Interactive Protein Design,
2021
Cancer Research @ UCC, University College Cork, Cork, Ireland; SynBioCentre, University College Cork, Cork, Ireland
Pepblock Builder Vr – An Open-Source Tool For Gaming-Based Bio-Edutainment In Interactive Protein Design, Venkata V. B. Yallapragada, Tianshu Xu, Sidney P. Walker, Sabin Tabirca, Mark Tangney
Cappa Publications
Proteins mediate and perform various fundamental functions of life. This versatility of protein function is an attribute of its 3D structure. In recent years, our understanding of protein 3D structure has been complemented with advances in computational and mathematical tools for protein modelling and protein design. 3D molecular visualisation is an essential part in every protein design and protein modelling workflow. Over the years, stand-alone and web-based molecular visualisation tools have been used to emulate three-dimensional view on computers. The advent of virtual reality provided the scope for immersive control of molecular visualisation. While these technologies have significantly improved our …
Plant Based Compounds Inhibit Proliferation, Alter Cytomorphology And Decrease Migration Of Human Adenocarcinoma Cells,
2021
Pittsburg State University
Plant Based Compounds Inhibit Proliferation, Alter Cytomorphology And Decrease Migration Of Human Adenocarcinoma Cells, Devapriya Segaran
Electronic Theses & Dissertations
Adenocarcinoma is an aggressive form of lung cancer that has a high risk of recurrence with a survival rate of 33%. In recent years, there has been much interest in the ability of naturally occurring plant derived phenols to inhibit specific type of cancers. Compounds like curcumin derived from turmeric, rutin derived from citrus fruits and resveratrol derived from blueberries have been of particular interest. In this thesis I studied the anti-cancer effects of the above-mentioned compounds on a human a549 adenocarcinoma cell line. Inverted phase contrast microscopy was used to observe alterations to the cytomorphology of cells. An MTT …
Impact Of Angiogenic And Osteogenic Factors In The Presence Of Biodegradable Piezoelectric Films,
2021
University of Connecticut
Impact Of Angiogenic And Osteogenic Factors In The Presence Of Biodegradable Piezoelectric Films, Jayla Millender
University Scholar Projects
One of the most common causes of bone graft rejection is lack of a vascular network connecting the graft to the existing native tissue – allowing for nutrient flow. Under current grafting techniques, the existing blood vessel network in the patient slowly invades the implant in order to supply the injured site with its necessary nutrients. The purpose of this research is to determine if a synthetic bone graft with a stable microvascular network can be developed in vitro. I hypothesize that the use of indirect angiogenic factors such as sonic hedgehog homolog and hypoxia-inducible factor-1 in combination with the …
Impact Of Angiogenic And Osteogenic Factors In The Presence Of Biodegradable Piezoelectric Films,
2021
University of Connecticut
Impact Of Angiogenic And Osteogenic Factors In The Presence Of Biodegradable Piezoelectric Films, Jayla Millender
Honors Scholar Theses
One of the most common causes of bone graft rejection is lack of a vascular network connecting the graft to the existing native tissue – allowing for nutrient flow. Under current grafting techniques, the existing blood vessel network in the patient slowly invades the implant in order to supply the injured site with its necessary nutrients. The purpose of this research is to determine if a synthetic bone graft with a stable microvascular network can be developed in vitro. I hypothesize that the use of indirect angiogenic factors such as sonic hedgehog homolog and hypoxia-inducible factor-1 in combination with the …
Extraction Of Human Dna From Soil In A Simulated Clandestine Grave,
2021
University of New Haven
Extraction Of Human Dna From Soil In A Simulated Clandestine Grave, Arely Joaly Parra Lopez
Honors Theses
Locard’s Exchange Principle states that “every contact leaves a trace.” The same principle applies when a perpetrator of a homicide decides to bury a victim in a clandestine grave. If a perpetrator originally buried a murder victim in haste to prevent discovery and then decided to later move the victim’s body to a remote location for disposal, decomposition fluids from the victim’s body (which contain cellular material and therefore DNA) would remain in the surrounding grave soil at the original burial site. It is possible that investigators could: 1) prove that a human body had once laid in that location …
Search For Palladin, An Actin-Associated Protein, In Pig Retinal Pigmented Epithelium And Its Role In Epithelial-Mesenchymal Transition,
2021
Bellarmine University
Search For Palladin, An Actin-Associated Protein, In Pig Retinal Pigmented Epithelium And Its Role In Epithelial-Mesenchymal Transition, Katrina Powell
Undergraduate Theses
This study investigates the expression of Palladin, a phosphoprotein product of the PALLD gene, in the retinal pigmented epithelium (RPE). Palladin is an actin cross-linking protein and plays a role in cell adhesion and motility. Published reports have demonstrated that a down regulation of Palladin in colon cancer cells results in a reorganization of the actin cytoskeleton, causing the cells to lose their typical shape, become proliferative and migratory. This process is otherwise known as epithelial-mesenchymal transition (EMT). A similar EMT phenomenon is observed when the RPE is exposed to the vitreous humor in patients with proliferative vitreoretinopathy (PVR). In …
Integrative Computational Approach Identifies Drug Targets In
Cd4+ T-Cell-Mediated Immune Disorders,
2021
University of Nebraska-Lincoln
Integrative Computational Approach Identifies Drug Targets In Cd4+ T-Cell-Mediated Immune Disorders, Bhanwar L. Puniya, Rada Amin, Bailee Lichter, Robert Moore, Alex Ciurej, Sydney J. Bennett, Ab Rauf Shah, Matteo Barberis, Tomáš Helikar
Department of Biochemistry: Faculty Publications
CD4+ T cells provide adaptive immunity against pathogens and abnormal cells, and they are also associated with various immunerelated diseases. CD4+ T cells’ metabolism is dysregulated in these pathologies and represents an opportunity for drug discovery and development. Genome-scale metabolic modeling offers an opportunity to accelerate drug discovery by providing high-quality information about possible target space in the context of a modeled disease. Here, we develop genome-scale models of naïve, Th1, Th2, and Th17 CD4+ T-cell subtypes to map metabolic perturbations in rheumatoid arthritis, multiple sclerosis, and primary biliary cholangitis. We subjected these models to in silico …
Safety Of Silver Oxide Coated Biomaterials In Mice,
2021
Rowan University
Safety Of Silver Oxide Coated Biomaterials In Mice, Michael Klug, Darien L. Seidman, Rahyan Mahmoud, Destiny Morot, Lei Yu, Jeffrey D Hettinger, Renee M Demarest
Rowan-Virtua Research Day
It has been demonstrated that silver oxide coatings designed by our collaborators are able to prevent E. coli and P. aeruginosa attachment to biomaterials in vivo. These findings demonstrate that such coatings show promise in preventing the development of biofilm on biodevices. However, it is unknown if the use of silver oxide in this fashion is toxic in vivo. The goal of this project was to determine whether our silver oxide coatings are safe to use in vivo. To assess the toxicity of our silver oxide formula, mice were implanted with either silver oxide coated titanium discs or uncoated titanium …
Agrobacterium Mediated Transformation Optimizations For Sugarcane (Saccharum Officinarum L.) Cultivar Spf-234 With Direct Organogenesis,
2021
Department of Botany, Government College University Faisalabad, Pakistan.
Agrobacterium Mediated Transformation Optimizations For Sugarcane (Saccharum Officinarum L.) Cultivar Spf-234 With Direct Organogenesis, Muhammad Nawaz, Naeem Iqbal, Rabia Hameed, Mehwish Mehwish, Shakra Jamil
Journal of Bioresource Management
Sugarcane (Saccharum officinarum L.) is the most important food and energy crop worldwide. In the present study, an efficient Agrobacterium mediated transformation and regeneration system for sugarcane cultivar SPF-234 was established. Agrobacterium tumefaciens strains EHA101and LBA4404 using vector pIG121 Hm, having GUS, HPTII and NPTII genes were used. Polymerase chain reaction (PCR) and histochemical assays confirmed the GUS gene expression. A 620 bp fragment from GUS positive plants was amplified. The GUS expressing putative transformants were 35% of the total plants formed under 30 minute immersion time and 72 hr of incubation period. The co-cultivation media having 60 …
Light Intensity Control For Plc-Based Hydroponic Demonstration System,
2021
University of Arkansas, Fayetteville
Light Intensity Control For Plc-Based Hydroponic Demonstration System, Christian Hitt
Biological and Agricultural Engineering Undergraduate Honors Theses
In order to demonstrate a skill set obtained through the University of Arkansas Biological Engineering, a group of students and I developed a hydroponic system where plants could be grown in a chamber. System parameters such as air quality, irrigation, and lighting were controlled by a Programmable Logic Computer (PLC) and an array of sensors and actuators. Each student focused on a certain aspect of this system, mine being the lighting. Developing this system involved researching the relationship between light intensity and plant growth, designing a system to suspend the lights above the growth chamber, and develop a system to …
Partial Expression Of The Vbss Gene In Rhizobium Leguminosarum Atcc 14479 And In-Silico Analysis Of The Vbs Gene Cluster In Various Microorganisms,
2021
East Tennessee State University
Partial Expression Of The Vbss Gene In Rhizobium Leguminosarum Atcc 14479 And In-Silico Analysis Of The Vbs Gene Cluster In Various Microorganisms, Afreen Siddiqui
Undergraduate Honors Theses
Iron is extremely important for many organisms. Despite its abundancy, it exists in insoluble forms that makes its usability difficult. Some organisms secrete siderophores, low molecular weight compounds, that can chelate iron and convert it into usable forms for cells. One such organism, Rhizobium leguminosarum, is a nitrogen fixing symbiont proteobacteria that infects leguminous plants. The genome of Rhizobium leguminosarum ATCC 14479, which infects the red clover, Trifoli pratense, has previously been completely sequenced in our lab. Our lab has identified several genes in this strain involved in the biosynthesis of a siderophore, vicibactin. The protein product of one of …
Biases And Blind-Spots In Genome-Wide Crispr-Cas9 Knockout Screens,
2021
The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences
Biases And Blind-Spots In Genome-Wide Crispr-Cas9 Knockout Screens, Merve Dede
Dissertations and Theses (Open Access)
Adaptation of the bacterial CRISPR-Cas9 system to mammalian cells revolutionized the field of functional genomics, enabling genome-scale genetic perturbations to study essential genes, whose loss of function results in a severe fitness defect. There are two types of essential genes in a cell. Core essential genes are absolutely required for growth and proliferation in every cell type. On the other hand, context-dependent essential genes become essential in an environmental or genetic context. The concept of context-dependent gene essentiality is particularly important in cancer, since killing cancer cells selectively without harming surrounding healthy tissue remains a major challenge. The toxicity of …
Preclinincal Development Of Avaren-Fc: A Novel Lectin-Fc Fusion Protein Targeting Cancer-Associated High-Mannose Glycans.,
2021
University of Louisville
Preclinincal Development Of Avaren-Fc: A Novel Lectin-Fc Fusion Protein Targeting Cancer-Associated High-Mannose Glycans., Matthew William Dent
Electronic Theses and Dissertations
This dissertation explores the anticancer activity of Avaren-Fc (AvFc), a novel lectin-Fc fusion protein or “lectibody” targeting cancer and virus-associated high-mannose glycans. Previously, we have shown that AvFc recognizes a broad selection of established cancer cell lines from a wide array of tissue types, can potently induce antibody-dependent cell-mediated cytotoxicity (ADCC) against them, and exhibits anti-cancer activity in vivo. However, the exact mechanism of action remains elusive. We hypothesized that the primary mechanism of action is through Fc-mediated effector functions, and the purpose of this dissertation is to explore this question through the use of Fc variants that either …
Unraveling Host-Gut Microbiota Dialogue And Its Impact On Response To Immune Checkpoint Blockade,
2021
The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences
Unraveling Host-Gut Microbiota Dialogue And Its Impact On Response To Immune Checkpoint Blockade, Alexandria Cogdill
Dissertations and Theses (Open Access)
Cancer is a disease with only one degree of separation, affecting one in two men and one in three women in their lifetimes; accounting for 1 of every 6 deaths. While cancer mortality rates continue to improve, incidence rates are expected to rise and shift through 2050 due to epidemiological and demographic transitions worldwide. As such, it is imperative to continue to investigate and improve our understanding of both disease etiology and hallmarks of response to treatment. Currently, conventional therapies include, but are not limited to, surgery, chemotherapy, and radiotherapy. However, within the past decade, major advances have been made …
Optimum Medium For Lipase Production By Lipolytic Filamentous Fungi Isolated From Kendari Landfill Soil,
2021
Universiti Brunei Darussalam
Optimum Medium For Lipase Production By Lipolytic Filamentous Fungi Isolated From Kendari Landfill Soil, Rayani Nur, Ilmi Miftahul
ASEAN Journal on Science and Technology for Development
Lipase produced by Aspergillus is widely known and used in many industrial sectors. In a previous study, three lipolytic filamentous fungi were isolated from Kendari (Southeast Sulawesi, Indonesia) landfill soil and identified as Aspergillus niger KE1, Aspergillus terreus KC1, and Aspergillus fumigatus KE6. However, the optimization of these isolates has not been reported. In this study, statistical optimization was selected because it is more effective, efficient, economical, and robust in achieving results, and the possibility of analyzing the interaction effects among factors. Three lipolytic isolates were screened in the initial medium to obtain the highest lipolytic isolate, which was used …
Investigation Of The Distribution Of The Daytime Employed Population Of Yangon, Myanmar, With And Without The Impact Of Work From Home,
2021
Universiti Brunei Darussalam
Investigation Of The Distribution Of The Daytime Employed Population Of Yangon, Myanmar, With And Without The Impact Of Work From Home, Aung Ye Wint, Myint San San
ASEAN Journal on Science and Technology for Development
Work from home (WFH) has gradually become more popular since COVID-19 started its transmission in Myanmar during the first quarter of 2020. Its consequence was a re-evaluation of the culture of workplaces and a significant change has occurred to the pattern of daytime employment distribution. In this paper, the network distribution model was built with three constraints, Distribution Bias 1, 2, and 3. These biases were determined through questionnaires, survey data collections of responses from Yangonites, and geographical data of urban employment cores. These percentile biases influenced the amount of employed population per distribution times and gave rise to two …
Understanding The Genetics Of Schizophrenia,
2021
Southern Maine Community College
Understanding The Genetics Of Schizophrenia, Matthew Toohey
Thinking Matters Symposium
Schizophrenia has been considered heritable for a long time, but only with the advent of new technologies such as whole-genome sequencing and genome-wide association studies can we begin to identify specific molecular causes of schizophrenia. This poster will review some of the genetic variants that research groups have associated with schizophrenia. Current research has indicated that schizophrenia is a polygenic disease and has been linked to many genes. Some of these common risk variants are in protein coding sections of the DNA. These proteins are often linked to neurological development or immune system function. Other variants that have been associated …
Anticipating Ocean Deoxygenation In The Maritime Continent Of Southeast Asia,
2021
Universiti Brunei Darussalam
Anticipating Ocean Deoxygenation In The Maritime Continent Of Southeast Asia, Wulandari Ita, Wahyudi A'An J., Triana Karlina
ASEAN Journal on Science and Technology for Development
Oxygen plays an essential role in the biogeochemical process and ocean productivity, especially during the recent trend of climate change. Development of the oxygen loss condition, deoxygenation, receives less attention than ocean acidification and warming. Therefore, understanding deoxygenation is indispensable. The maritime continent waters of Southeast Asia (SEA) are well-known for marine biodiversity and unique geological features. The area is inevitably impacted by climate change and will suffer more due to less oxygen in seawater. Based on previous research, Bengal Bay has been affected by oxygen depletion and climate change, in which hypoxic rate conditions continuously increase. In the other …
