Extraction Of Cannabidiol From Hemp Leaf Using Supercritical Fluid Technique And Production Of Cannabidiol Emulsion Microencapsule By Freeze Drying,
2024
Faculty of Science
Extraction Of Cannabidiol From Hemp Leaf Using Supercritical Fluid Technique And Production Of Cannabidiol Emulsion Microencapsule By Freeze Drying, Saranya Jansamutr
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to extract, purify, formulate, and evaluate the bioactivities of cannabidiol (CBD) derived from hemp (Cannabis sativa L.) leaves. Supercritical carbon dioxide (SC-CO2) extraction at pressures of 1,600–1,800 psi, combined with flash chromatography, was employed to obtain CBD-rich extracts. Higher extraction pressure significantly increased both yield and CBD concentration (p ≤ 0.05), with 1,800 psi selected for further formulation. The extracts were developed into emulsions and microcapsules using freeze-drying and ß-cyclodextrin as an encapsulating agent to enhance chemical stability. Emulsions were formulated using MCT oil, Tween 20, and lecithin in various ratios including 9:5:5 (formula 1), 8:4:4 (formula …
Application Of Mycelia Inoculum Of Ectomycorrhizal Fungus Astraeus Odoratus For Dry Dipterocarp Forest Restoration And Inoculum Development By Mycelial Entrapment With Calcium Alginate,
2024
Faculty of Science
Application Of Mycelia Inoculum Of Ectomycorrhizal Fungus Astraeus Odoratus For Dry Dipterocarp Forest Restoration And Inoculum Development By Mycelial Entrapment With Calcium Alginate, Yanisa Punsung
Chulalongkorn University Theses and Dissertations (Chula ETD)
Dry Dipterocarp Forests (DDFs) in Thailand face severe degradation, primarily from human-induced fires, which significantly diminishes ectomycorrhizal (ECM) fungal diversity and the productivity of valuable wild mushrooms, such as Astraeus odoratus. However, concrete guidelines for restoring ECM fungal diversity in these degraded areas are currently lacking. This research aimed to develop a sustainable approach to enhance ECM fungal diversity and facilitate the restoration of degraded DDFs by utilizing A. odoratus mycelia. Furthermore, we developed alginate-encapsulated A. odoratus mycelial beads to extend inoculum viability and applicability. The study was conducted at two degraded DDF sites: Ban Kor Thung and Ban Mae …
Byproducts Of Tropical Marine Processing As A Candidate For Industrial Food Ingredients: An Appraisal On Macro And Micronutrients,
2024
Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia
Byproducts Of Tropical Marine Processing As A Candidate For Industrial Food Ingredients: An Appraisal On Macro And Micronutrients, Abdul Aziz Jaziri, Hajariah Hasanuddin, Rossita Shapawi, Ruzaidi Azli Mohd Mokhtar, Wan Norhana Md. Noordin, Masmunira Rambli, Nurul Huda
ASEAN Journal on Science and Technology for Development
Tropical marine fish is a main raw material for surimi processing plants, including golden threadfin bream (Nemipterus virgatus Houttuyn, 1782) and swallowtail dwarf monocle bream (Parascolopsis eriomma Jordan & Richardson, 1909). After processing, more than 60% of fish are considered as byproducts (i.e., head, viscera, skin, bones, fins and scale), and tend to discard due to poor-utilization. Hence, this paper aims to evaluate macro- and micro-nutrients derived from golden threadfin bream (GTB) and swallowtail dwarf monocle bream (SDMB). The higher content of protein (25.31 ± 0.81%) was observed in the skin part of GTB, followed by the flesh (21.26 ± …
Nanocellulose Synthesized From Sugarcane Bagasse (S. Officinarum) Via Alkaline-Mechanical Process And Its Characterization,
2024
Food Science and Technology, School of Applied Sciences and Mathematics, Universiti Teknologi Brunei, Gadong, BE1410, Bandar Seri Begawan, Brunei Darussalam
Nanocellulose Synthesized From Sugarcane Bagasse (S. Officinarum) Via Alkaline-Mechanical Process And Its Characterization, Wafiqah Daim, Hiroshi Uyama, Syazana Abdullah Lim
ASEAN Journal on Science and Technology for Development
Nanocellulose is one of many promising materials that have garnered the attention of researchers and industries. In this study, sugarcane bagasse (S. officinarum) was used to synthesize cellulose nanofiber (SB-CNF) via an alkaline-mechanical process. A comprehensive characterization of SB-CNF was conducted to explore its morphology via scanning electron microscopy (SEM) and dynamic force microscopy (DFM), assess its functional groups using Fourier transform infrared spectroscopy (FT-IR), analyze its thermal properties through thermogravimetric analysis (TGA), and lastly, evaluate light absorbance and transmittance using a UV-VIS spectrophotometer. Our findings revealed that SB-CNF resulted in a highly intertwined nanofiber structure within a nano-scale dimension. …
Smes’ Perspectives On The Benefits And Challenges Of Halal Certificate Application In Brunei Darussalam,
2024
School of Business, Universiti Teknologi Brunei
Smes’ Perspectives On The Benefits And Challenges Of Halal Certificate Application In Brunei Darussalam, Kamariah Ismail, Syahnur Farhana Haji Shahlehi, Vivi Nabilah Shaya
ASEAN Journal on Science and Technology for Development
The knowledge and research on SMEs and Halal-related studies in Brunei Darussalam are scant. Guided by the principles of Resource-Based View theory, this qualitative study aims to identify the benefits and challenges of Halal certificate application from the perspectives of Bruneian Halal food SMEs, as they obtain the Brunei’s Halal certificate and label. Based on the purposive and snowball sampling, four semi-structured interviews were conducted with business owners and managers in the Halal food industry. Government officials were also in-terviewed to enhance the findings. Thematic analysis, supplemented by secondary data, was employed for a comprehensive understanding. Four themes emerged, covering …
Innovation Barriers Obstructing The Transfer Of Technology From University To Industry In Asean Developing Countries (Case Study On Brunei Darussalam),
2024
Graduate, School of Business, Universiti Teknologi Brunei
Innovation Barriers Obstructing The Transfer Of Technology From University To Industry In Asean Developing Countries (Case Study On Brunei Darussalam), Rafiq Zaini, Kamariah Ismail
ASEAN Journal on Science and Technology for Development
This study aims to explore the barriers of innovation in one of ASEAN developing countries particularly Brunei Darussalam. This research focuses on 3 sectors which are from 1) the government of Brunei to 2) the higher education institutions particularly Universiti Teknologi Brunei to 3) industry sectors in Brunei Darussalam - primarily in the processes of technology transfer. Using the Triple Helix theory model, barriers were identified between consecutive sectors. In this study, qualitative research methods have been utilized (case studies using interview methods) and a total of 9 main barriers have been identified between the sectors from the literature review …
Does The Foreign Direct Investment In Emerging Asian Countries Get ‘Greased’ Or ‘Sanded’ By Corruption? Evidence From Gets Model,
2024
Faculty of Business and Management, Universiti Teknologi MARA Sabah Branch, Kota Kinabalu Campus, Malaysia
Does The Foreign Direct Investment In Emerging Asian Countries Get ‘Greased’ Or ‘Sanded’ By Corruption? Evidence From Gets Model, Rainah Ginsad, Imbarine Bujang, Abdul Aziz Karia, Emelia Girau, Siti Rahayu Beli
ASEAN Journal on Science and Technology for Development
Inflows of foreign direct investment (FDI) considerably boost industrialization and economic growth in emerging economies. Corruption, human capital, and the economic environment are just a few factors mentioned in the literature as influencing FDI inflow. The impact of corruption on FDI, whether FDI is greased or sanded by corruption, has received greater interest in recent empirical study. This study analysed the impact of the Corruption Perception Index (CPI) on foreign direct investment (FDI) in selected Asian emerging economies. Utilising annual panel data covering 22 selected Asian emerging economies, this study uses the General-to-specific (GETS) model to analyse the impact. The …
Large T Antigen Mediated Target Gene Replication Improves Site-Specific Recombination Efficiency,
2024
The Texas Medical Center Library
Large T Antigen Mediated Target Gene Replication Improves Site-Specific Recombination Efficiency
Faculty, Staff and Student Publications
With advantages of high-fidelity, monoclonality and large cargo capacity, site-specific recombination (SSR) holds great promises for precise genomic modifications. However, broad applications of SSR have been hurdled by low integration efficiency, and the amount of donor DNA available in nucleus for SSR presents as a limiting factor. Inspired by the DNA replication mechanisms observed in double-stranded DNA virus SV40, we hypothesized that expression of SV40 large T antigen (TAg) can increase the copy number of the donor plasmid bearing an SV40 origin, and in consequence promote recombination events. This hypothesis was tested with dual recombinase-mediated cassette exchange (RMCE) in suspension …
One-Step Production Of Fully Biotinylated And Glycosylated Human Fc Gamma Receptors,
2024
The Texas Medical Center Library
One-Step Production Of Fully Biotinylated And Glycosylated Human Fc Gamma Receptors
Faculty, Staff and Student Publications
Initiating and regulating humoral immunity, Fc gamma receptors (FcγRs) have been identified both as therapeutics and as drug targets, and thus production of biologically active FcγRs is highly demanded for biopharmaceutical development. Focusing on low-affinity FcγRs IIA (131H/R allotypes), IIB, and IIIA (176F/V), this study used human 293-F cells to achieve correct post-translational modifications (PTMs) including biotinylation, N-glycosylation, and disulfides. Approaches involving co-expression of FcγR-AviTag and Escherichia coli biotin ligase BirA, endoplasmic reticulum retention, stable and transient transfections, and optimization of transgene ratio were investigated. Protein electrophoresis under reducing and non-reducing conditions, enzymatic deglycosylation, streptavidin pull-down assays, and binding kinetic …
Fungal Activity In Fusarium Keratoplasticum Isolates With And Without Endohyphal Bacteria On Various Carbon Sources,
2024
Georgia Southern University
Fungal Activity In Fusarium Keratoplasticum Isolates With And Without Endohyphal Bacteria On Various Carbon Sources, Maya Brown
Honors College Theses
In recent years, the fungus Fusarium keratoplastic has been associated with failed sea turtle eggs. One fungal isolate collected from a failed egg collected on Wassaw Island, GA in 2020 contained endohyphal bacteria, bacteria living within the hyphae of the fungus. The type of endohyphal bacteria (EHB), as well as the relationship between the bacteria and fungal hosts, are currently unknown. Previous research on the same fungus and EHB, specifically Chitinophaga sp., discovered that EHB influenced the ability of the fungus to utilize carbon sources. Our research goal was to examine the metabolic relationship of Fusarium isolated from failed sea …
Developing Herbal-Based Beverage Fermentation Using Saccharomyces Cerevisiae: The Physico-Chemical Properties,
2024
School of Applied Sciences and Mathematics, Universiti Teknologi Brunei, Mukim Gadong A, Brunei Darussalam
Developing Herbal-Based Beverage Fermentation Using Saccharomyces Cerevisiae: The Physico-Chemical Properties, Siti Madihah Mohd Don, Mas Munira Rambli, Beston Faiek Nore
ASEAN Journal on Science and Technology for Development
Herbal-based fermented beverages are popular non-dairy products among consumers who seek healthy and immunity-supporting products. Amongst commonly used health supplements that are added to food and beverage would be probiotics, commonly Saccharomyces cerevisiae or yeast. This study aims to determine the physico-chemical properties of fermented beverages at different fermentation periods using lemongrass (Cymbopogon citratus DC. Stapf), ginger (Zingiber officinale Rosc.), turmeric (Curcuma longa L.), mint (Mentha), and moringa (Moringa oleifera Lam.). This study revealed a significant decrease (p ≤ 0.05) in pH, total soluble solids, and density in most fermented beverages after 24, 36 and 48 hours of fermentation. Low …
Technological Advancements In Brunei’S Local Agriculture And Fisheries Companies: An Exploratory Study Based On Field Observations,
2024
School of Applied Sciences and Mathematics, Universiti Teknologi Brunei, Gadong, BA1410, Bandar Seri Begawan, Brunei Darussalam
Technological Advancements In Brunei’S Local Agriculture And Fisheries Companies: An Exploratory Study Based On Field Observations, Muhammad Firdaus Mujibuddin Syah Mustafa, Navaranjan Namasivayam, Hong Shyang Pei, Fatnin Farhanah Alihan, Amni Sahilah Sahrullamzah, Khairina Umairah Takiyaudin
ASEAN Journal on Science and Technology for Development
This exploratory study, through seven field observations and interviews, investigates the technological advancements transforming Brunei's agricultural landscape in pursuit of local production augmentation and self-sufficiency. It confronts challenges with emerging technologies like aquaponics, hydroponics, fertigation systems, closed-house poultry systems, and UV lighting, which hold promise for enhanced farming practices. The research provides a comprehensive assessment of current practices, including livestock management advancements, underscoring their potential to boost productivity, optimize resource use, and promote sustainable agriculture. Furthermore, it discusses the broader implications for industry standards, job employability, and alignment with Brunei's Vision 2035. The findings contribute invaluable insights, advocating for strategic …
Exploring The Impact Of Vented Packaging Configurations On Airflow Patterns And Cooling Consistency For Muskmelons In A Cold Storage Environment,
2024
School of Applied Sciences and Mathematics, Universiti Teknologi Brunei, Bandar Seri Begawan, Brunei Darussalam
Exploring The Impact Of Vented Packaging Configurations On Airflow Patterns And Cooling Consistency For Muskmelons In A Cold Storage Environment, Nur Wafidah Roslan, Namasivayam Navaranjan, Zohrah Sulaiman, Naseem Uddin
ASEAN Journal on Science and Technology for Development
The cooling effect of different vented packaging designs was investigated for packed muskmelons fruits in a commercial cold storage condition. Computational fluid dynamics (CFD) simulation and experiments were used. The airflow pattern, heat transfer and temperature profiles were predicted from simulation and analysed. The CFD results reveal an uneven and low efficient airflow distribution inside a commercially used vented packaging (Box A). This resulted slow cooling of the fruits in Box A. Two newly designed packaging (Box B and Box C) showed better airflow distribution around the fruits and cooled them faster than Box A. The design of Box C …
Physicochemical Characterization Of Edible Insect Oils: Insights Into Fatty Acid Composition, Thermal Behavior And Quality Parameters,
2024
School of Applied Sciences and Mathematics, Universiti Teknologi Brunei, Bandar Seri Begawan, Brunei Darussalam
Physicochemical Characterization Of Edible Insect Oils: Insights Into Fatty Acid Composition, Thermal Behavior And Quality Parameters, Eng-Tong Phuah, Yee-Ying Lee, Teck-Kim Tang, Guanghui Li, Shyang-Pei Hong, Syazana Abdullah Lim
ASEAN Journal on Science and Technology for Development
Edible insects are emerging as a sustainable food source due to their high nutritional value. This study employed the three-phase partitioning (TPP) method to extract oils from sago worm (Rhynchophorus ferrugineus), superworm (Zophobas morio), and cricket (Acheta domesticus). The study evaluated and compared the physicochemical properties of these insect oils, including iodine value (IV), peroxide value (PV), p-anisidine value (p-AV), acid value (AV), refractive index (RI), thermal behavior, and fatty acid compositions. Results revealed that the sago worm exhibited the highest oil yield (47.8 ± 2.2%) compared to the superworm (23.8 ± 1.4%) and cricket (6.4 ± 0.6%). Cricket oil …
Recent Advance In Biodegradable Packaging From Banana Plant Feedstock: A Comprehensive Review,
2024
School of Applied Sciences and Mathematics, Universiti Teknologi Brunei, Bandar Seri Begawan, Brunei Darussalam
Recent Advance In Biodegradable Packaging From Banana Plant Feedstock: A Comprehensive Review, M. Atiq Juani, Namasivayam Navaranjan
ASEAN Journal on Science and Technology for Development
This review focuses on recent advances in biodegradable packaging derived from banana plant waste, highlighting its pivotal role in addressing environmental concerns linked to conventional packaging materials. The shift toward sustainable packaging stems from the urgent need to combat plastic pollution, reduce reliance on non-renewable resources, and foster sustainability in the food industry. Banana plants, known for generating significant organic waste during cultivation and processing, provide a valuable source for developing biodegradable packaging. Researchers have successfully transformed banana waste into innovative, recyclable, and eco-friendly packaging solutions, promoting a circular economy. Biodegradable packaging, in contrast to traditional materials derived from fossil …
Evaluation Of Physicochemical Properties Of Chicken Sausages Containing Whey Protein Isolate And Guar Gum,
2024
School of Applied Sciences and Mathematics, Universiti Teknologi Brunei, Bandar Seri Begawan, Brunei Darussalam
Evaluation Of Physicochemical Properties Of Chicken Sausages Containing Whey Protein Isolate And Guar Gum, Nur Hamizah Hamid, Namasivayam Navaranjan
ASEAN Journal on Science and Technology for Development
The present work aims to evaluate the physicochemical properties and proximate composition of adding whey protein isolate (WPI) and guar gum (GG) as emulsion gels in chicken sausages formulated with different fat percentages. The fat reduction resulted in products having lower moisture content (p
Unlocking Antioxidant Potential: Comparative Analysis Of Sembada 188 And Keladi Rice As Functional Ingredients In Novel Food Production,
2024
Centre for Research on Agri-Food Science and Technology, Universiti Teknologi Brunei, Bandar Seri Begawan, Brunei Darussalam
Unlocking Antioxidant Potential: Comparative Analysis Of Sembada 188 And Keladi Rice As Functional Ingredients In Novel Food Production, Mas Munira Rambli, Natasha Azamain, Eng-Tong Phuah
ASEAN Journal on Science and Technology for Development
Rice (Oryza sativa L.) is an essential crop that is a dietary staple for more than half of the world's population. There is currently a movement in consumer behaviour toward healthier food consumption, which is fuelling interest in functional diets like coloured rice. Sembada 188 and Keladi rice are underutilised in Brunei Darussalam and are expected to have functional qualities. A comparison of antioxidant properties between Sembada 188 and Keladi rice was performed in this study. This research produced puffed rice treats prepared by steaming rice, washing, drying for 6-8 hours at 60℃, deep frying for 25 seconds at 180℃, …
Preliminary Study On The Effects Of Vinegar As Pre- Treatment For The Oven-Drying Of Pacific Yellowtail Emperor (Lethrinus Atkinsoni) Fillets,
2024
Mindanao State University Tawi-Tawi College of Technology and Oceanography, Tawi-Tawi, Philippines.
Preliminary Study On The Effects Of Vinegar As Pre- Treatment For The Oven-Drying Of Pacific Yellowtail Emperor (Lethrinus Atkinsoni) Fillets, Nurisa A. Suhaili, Rafael S. Jamih, Normina A. Abubakar, Jaro O. Ajik, Merilyn Q. Amlani
ASEAN Journal on Science and Technology for Development
This study aimed to assess the impact of various vinegar compositions used as pre-treatment for Pacific Yellowtail Emperor (PYE) fillets during the oven-drying process, with a focus on moisture content and optimal drying conditions. Two types of commercially available vinegar, Superior Vinegar and Datu Puti Vinegar, were compared, and different drying temperatures were evaluated. The investigation revealed that the drying temperature significantly influenced the moisture content of the dried PYE fillets. Among the tested temperatures (40°C, 60°C, and 80°C), the most favorable outcome in terms of moisture content was achieved when fillets were dried at 80°C for a duration of …
Application Of Three Of Selected Medicinal Plant Extracts In Brunei Darussalam To Improve Shelf Life Of Poultry Product,
2024
School of Applied Sciences and Mathematics, Universiti Teknologi Brunei, Gadong, BA1410, Bandar Seri Begawan, Brunei Darussalam
Application Of Three Of Selected Medicinal Plant Extracts In Brunei Darussalam To Improve Shelf Life Of Poultry Product, Hazirah Abd Hamid, Namasivayam Navaranjan, Zohrah Sulaiman, Aida Maryam Binti Hj Basri
ASEAN Journal on Science and Technology for Development
Abstract. Bioactive compounds from plant derived in food application become increasingly in demand due to natural and nutritionally safe products. Due to some issues concerning food safety on the excessive use of synthetic preservatives; the present study aims to investigate the natural alternatives to synthetic antimicrobial food additives. The chicken meat’s shelf life was studied by adopting bio-based antimicrobial agents using three of selected native medicinal plants; P. betle, A. catechu, and A. tricolor, against common foodborne pathogens; E. coli, P. aeruginosa, S. aureus, and Salmonella spp. The overall results revealed that, all study extracts possessed antioxidant properties by DPPH …
Prevalence Of Resistant Escherichia Coli Isolated From Local Meats Sold In Tema Metropolis, Ghana,
2024
Department of Animal Science, Faculty of Agriculture, Food and Consumer Sciences, University for Development Studies, Tamale, Ghana
Prevalence Of Resistant Escherichia Coli Isolated From Local Meats Sold In Tema Metropolis, Ghana, Frederick Adzitey, Innocent Allan Anachinaba, Charles Addo-Quaye Brown, Nurul Huda, Masmuniri Rambli
ASEAN Journal on Science and Technology for Development
Contamination of meat by Escherichia coli (E. coli) can take its source from the live animal or by cross contamination. The study determined the prevalence of resistance of E. coli isolated from locally produced meats in the Tema Metropolis. Beef (n=200), chicken (n=200) and pork (n=200) were randomly selected and evaluated for the presence of E. coli using the procedure in the Bacteriological Analytical Manual of USA-FDA. The disc diffusion method was used for antibiotic susceptibility test of E. coli (n=55) isolates. Locally produced beef (67%), chicken (41%) and pork (23%) were contaminated by E. coli. Escherichia coli isolates were …
