Biotechnology Commons^™

Physical And Mechanical Properties Of Jute Mat Reinforced Epoxy Composites, Sadaf S.M, Siddik M, Ahsan Q

ASEAN Journal on Science and Technology for Development

Cellulose jute fibre offers a number of benefits as reinforcement for synthetic polymers since it has a high specific strength and stiffness, low hardness, relatively low density and biodegradability. To reduce moisture uptake and hence to improve the mechanical properties of the composites, bleached jute mats were incorporated as reinforcing elements in the epoxy matrix. Composites at varying volume fractions and different orientations of jute mat were fabricated by hot compression machine under specific pressures and temperatures. Tensile, flexure, impact and water absorption tests of composites were conducted. Jute mat oriented at (0 ± 45–90)° composites showed reduced strength compared …

Nutrient Composition Of Artocarpus Champeden And Its Hybrid (Nanchem) In Negara Brunei Darussalam, Lim L.B.L, Chieng H.I, Wimmer F.L

ASEAN Journal on Science and Technology for Development

The flesh and seeds of ripened and unripened Artocarpus champeden and its ripened hybrid (Nanchem) were analyzed for their moisture, ash, crude fibre, crude protein, crude fat, total carbohydrate, energy and mineral content. Generally, unripened A. champeden which is always treated and cooked as a vegetable contains higher amounts of moisture, ash, crude fibre and crude protein for its flesh than ripened A. champeden and Nanchem. Ripened A. champeden and Nanchemhave higher total carbohydrates and energy content than the unripe fruit. Similarly, the unripened A. champeden seed has more nutritional components in terms of moisture, ash, crude fibre, crude protein, …

Field And Laboratory-Based Approach For The Determination Of Friction Angle Of Geological Discontinuities Of Malaysian Granites, Ghani R. A, Goh T. L, Hariri A. M, Baizura Y. N

ASEAN Journal on Science and Technology for Development

The basic friction angle, Φb for artificially sawn discontinuity planes for fresh granite, as determined by tilt testing, has an average value of 30º. For the natural rough discontinuity surfaces, a wide range of values have been determined for the peak friction angle, Φpeak ranging from 47º to a maximum value of 80º, depending on the joint roughness coefficient (JRC). The average values of the friction angles for the different degrees of roughness were as follows: JRC 2–4 = 58°; JRC 6–8 = 60°; JRC 8–10 = 47°; JRC 12–14 = 60°; JRC 14–16 = 71° ; JRC 18–20 = …

Usage Of Uasb Reactor To Assess Feasibility Of Treatment Of Paper Mill Effluent, Arshad A, Hashim N.H, Ghazala N

ASEAN Journal on Science and Technology for Development

Upflow anaerobic sludge blanket (UASB) reactors R-I and R-II, each with an effective volume of 6.0 l were used to study the treatability of actual effluent obtained from paper mills at a mesophilic temperature and neutral pH. Methanol, as a source of an easily biodegradable substance along with activated carbon of effective size 1.5 mm–2.5 mm were added to the reactor R-I to a total depth of 12 cm to evaluate its efficiency. The pH of both the reactors were kept constant at neutral by adding an external buffer solution of 0.03 M NaHCO3 with the feed solution. It was …

Assessment Of The Treatment Of Textile Mill Effluent Using Uasb Reactor, Arshad A, Hashim N.H, Kashif A.K, Bashir A

ASEAN Journal on Science and Technology for Development

This study was designed to evaluate the feasibility of the treatment of actual textile mill effluent using a upflow anaerobic sludge blanket (UASB) reactor. The main objective of this study was to generate design aids; in terms of organic loading rate (OLR), hydraulic retention time (HRT) versus chemical oxygen demand (COD) and colour removal in the textile effluent using a UASB reactor at neutral pH and constant mesophilic temperature. The COD, colour and total suspended solids concentration of the textile wastes used in the study were analyzed as 5440 mg/l, 3280 mg/l, 2320 units and 955 mg/l, respectively. The UASB …

Programmers And Forensic Analyses: Accusers Under The Confrontation Clause, Karen Neville

Duke Law & Technology Review

Recent Supreme Court cases involving the Confrontation Clause have strengthened defendants’ right to face their accusers. Bullcoming v. New Mexico explored the question of whether the testimony of the technician who performs a forensic analysis may be substituted by that of another analyst, and the Court held that producing a surrogate witness who was not sufficiently involved in the analysis violates the confrontation right.

The presumption of infallible technology is fading, and courts may soon realize programmers have greater influence over the ultimate outcome of forensic tests than do the technicians who rely on such analytical tools. The confrontation right, …

Boron Stress Activates The General Amino Acid Control Mechanism And Inhibits Protein Synthesis, Irem Uluisik, Alaattin Kaya, Dmitri E. Fomenko, Huseyin C. Karakaya, Bradley A. Carlson, Vadim N. Gladyshev, Ahmet Koc

Department of Biochemistry: Faculty Publications

Boron is an essential micronutrient for plants, and it is beneficial for animals. However, at high concentrations boron is toxic to cells although the mechanism of this toxicity is not known. Atr1 has recently been identified as a boron efflux pump whose expression is upregulated in response to boron treatment. Here, we found that the expression of ATR1 is associated with expression of genes involved in amino acid biosynthesis. These mechanisms are strictly controlled by the transcription factor Gcn4 in response to boron treatment. Further analyses have shown that boron impaired protein synthesis by promoting phosphorylation of eIF2α in a …

Laboratory Diagnosis Of Clostridium Difficile Infection: Can Molecular Amplification Methods Move Us Out Of Uncertainty?, Fred C. Tenover, Ellen Jo Baron, Lance R. Peterson, David Persing

Biology Faculty Publications

The laboratory diagnosis of Clostridium difficile infection (CDI) continues to be challenging. Recent guidelines from professional societies in the United States note that enzyme immunoassays for toxins A and B do not have adequate sensitivity to be used alone for detecting CDI, yet the optimal method for diagnosing this infection remains unclear. Nucleic acid amplification tests (NAATs) that target chromosomal toxin genes (usually the toxin B gene, tcdB) show high sensitivity and specificity, provide rapid results, and are amenable to both batch and on-demand testing, but these tests were not universally recommended for routine use in the recent guidelines. …

Iron Insufficiency Compromises Motor Neurons And Their Mitochondrial Function In Irp2-Null Mice, Suh Young Jeong, Daniel R. Crooks, Hayden Wilson-Ollivierre, Manik C. Ghosh, Rachid Sougrat, Jaekwon Lee, Sharon Cooperman, James B. Mitchell, Carole Beaumont, Tracey A. Rouault

Department of Biochemistry: Faculty Publications

Genetic ablation of Iron Regulatory Protein 2 (Irp2, Ireb2), which post-transcriptionally regulates iron metabolism genes, causes a gait disorder in mice that progresses to hind-limb paralysis. Here we have demonstrated that misregulation of iron metabolism from loss of Irp2 causes lower motor neuronal degeneration with significant spinal cord axonopathy. Mitochondria in the lumbar spinal cord showed significantly decreased Complex I and II activities, and abnormal morphology. Lower motor neurons appeared to be the most adversely affected neurons, and we show that functional iron starvation due to misregulation of iron import and storage proteins, including transferrin receptor 1 …

Identification And Characterization Of A Novel Thermohalophilic Esterase From The Red Sea: Atlantis Ii Brine Pool, Yasmine Mustafa Mohamed

Theses and Dissertations

Industrial (white) biotechnology poses an increasing demand for novel biocatalysts that are robust under a wide range of conditions. Traditionally, biocatalysts were isolated from cultured isolates, however, less than 1% of microorganisms are culturable. Therefore, it became evident that the unculturable majority holds a great potential for the discovery of novel biocatalysts. Metagenomics is an invaluable tool for accessing the genomes of the uncultured majority and has led to the isolation of a large number of biocatalysts from various environments. Extreme environments such as hydrothermal vents, brine pools and glaciers, are an attractive source for biocatalysts. Biocatalysts from these environments …

Library Impact Statement For Mls 496 Global Health, Michael Cerbo

Library Impact Statements

Library Impact Statement in response to new course proposal for MLS 496 Global Health. New course was supported with no need for additional resources.

Library Impact Statement For Mls 575 Business Of Biotechnology, Michael Cerbo

Library Impact Statements

Library Impact Statement in response to new course proposal for MLS 575 Business of Biotechnology. New course was supported with no need for additional resources.

Library Impact Statement For Mls 572 Vaccines And Biologics, Michael Cerbo

Library Impact Statements

Library Impact Statement in response to new course proposal for MLS 572 Vaccines and Biologics. New course was supported with no need for additional resources.

Library Impact Statement For Mls 573 Molecular Biology Methods, Michael Cerbo

Library Impact Statements

Library Impact Statement in response to new course proposal for MLS 573 Molecular Biology Methods. New course was supported with no need for additional resources.

Activation Of Jnk Signaling Mediates Amyloid-Ss- Dependent Cell Death, Meghana Tare, Rohan Modi, Jaison Nainaparampil, Oorvashi Roy Puli, Shimpi Bedi, Pedro Fernandez-Funez, Madhuri Kango-Singh, Amit Singh

Biology Faculty Publications

Background: Alzheimer's disease (AD) is an age related progressive neurodegenerative disorder. One of the reasons for Alzheimer's neuropathology is the generation of large aggregates of Aß42 that are toxic in nature and induce oxidative stress, aberrant signaling and many other cellular alterations that trigger neuronal cell death. However, the exact mechanisms leading to cell death are not clearly understood.

Methodology/Principal Findings: We employed a Drosophila eye model of AD to study how Aß42 causes cell death. Misexpression of higher levels of Aß42 in the differentiating photoreceptors of fly retina rapidly induced aberrant cellular phenotypes and cell death. We found that …

Propagation System Manual, Margaret Kane

Hydroponic Center

Contained in this manual you will find the required materials, assembly instructions, operation procedures and safety protocols to construct, run and maintain a hydroponic propagation system.

The Propagation System is designed to provide an environment for seeds to germinate with optimal conditions for seedling growth. The purpose of the Propagator is to create an environment that is suitable for seedlings to develop a strong root system and uprooted stem so they can be transplanted into a new system where they are able to fully develop and be harvested. This procedure introduces plants, vegetables, and fruits to a new system of …

Studies Of Food Thickeners In Nigeria For Contamination By Aflatoxigenic Forms Of Aspergillus And Their Detection By Pcr, G. I. Okwu, Premila Achar, Michael J. Ikenebomeh, S. Y. Sreenivasa

Faculty Articles

This paper reports the contamination of ready-to-use food thickeners, collected from the South-East geo-political zone in Nigeria, by aflatoxigenic form of Aspergillus species. A total of 150 samples from different open markets were observed for fungal contamination by using serial dilution-spread plate method. Although, Aspergillus, Fusarium and Penicillium were the most frequently isolated fungi, Aspergillus species were found to be the most prevalent in all the samples. Furthermore, Aspergillus flavus and Aspergillus parasiticus produced aflatoxin on yeast extract sucrose (YES) media incubated for 10 to 15 days at 27°C in a CO₂ incubator. Aspergillus niger showed no sign of …

Forward Osmosis Bag: Ground Testing The Prospect Of Using Alternative Water Sources For Drinking Water, Michaela Marie Brant, Ariella Yendler, Mona Hammoudeh, Michael T. Flynn

STAR Program Research Presentations

On the STS-135 Space Shuttle mission, to be launched July 8, 2011, a forward osmosis bag (FOB) study will be conducted. At NASA Ames this summer, the ground truth testing is being conducted for results comparisons. The FOB technology is derived from a commercial product, the X-Pack water filter. Forward osmosis operates by utilizing an established concentration gradient across a semi-permeable membrane to move water molecules from one side of the membrane to the other. This concept is exploited to harvest drinking water from grey water sources such as urine, sea water, or vehicle water. In this experiment, potassium chloride …

The Proteomic Response Of Ciona Intestinalis To Ocean Acidification And Acute Heat Stress: Impacts Of Global Climate Change In Coastal Marine Systems, Michael Dennis Dwyer Jr.

Biological Sciences

No abstract provided.

Applied And Mechanistic Studies Of Microbial 17beta-Estradiol Degradation, Zhongtian Li

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

The presence of natural estrogens, a class of endocrine disrupting compounds, in water has caused increasing concerns over their adverse impacts on the health of aquatic eco-systems and human beings. In this study, adsorption characteristics of two natural estrogens, 17β-estradiol (E2) and estrone (E1), on granular activated carbon (GAC) were investigated in isotherm tests and in a GAC column. The GAC column was then converted to a biologically active carbon (BAC) column and the removal efficiency of E2 and its primary biodegradation intermediate E1 were monitored. During BAC operation, the impacts of various reactor operation parameters, such as the carbon …