Analyzing Environmental Microbes For Genomic Regions Promoting Ionic Liquid Tolerance In E. Coli,
2012
California State University - East Bay, San Jose High School
Analyzing Environmental Microbes For Genomic Regions Promoting Ionic Liquid Tolerance In E. Coli, Ann Nguyen, Alison Richins, Thomas Rüegg, Steven Singer, Michael Thelen
STAR Program Research Presentations
Ionic liquids (ILs) are promising as solvents to increase the efficiency of biofuel production; however, ILs are toxic to microbes used in the fermentation of liquid fuels. To engineer IL resistant biofuel hosts, environmental bacteria were screened for tolerance, and these were used to create gene libraries to test in E. coli. Future characterization of these libraries using molecular techniques will be used to identify genes that contribute IL-tolerance to transformed microbes.
Diffusion Barriers Of The Lateral Ventricular System,
2012
University of Connecticut
Diffusion Barriers Of The Lateral Ventricular System, Anitha Saravanakumar
Master's Theses
Adult neurogenesis in mammalian brain has become a well-accepted phenomenon. The neural stem cells in the sub ventricular zone (SVZ) along the lateral wall of the lateral ventricle, the largest germinal center of adult neurogenesis, are maintained by the neural stem cell niche. One prominent feature of the niche is its close proximity to the cerebral spinal fluid (CSF). The role of micromolecules (such as ions, water and gases) present in the CSF and diffusing through the lateral ventricular wall and how the stem cell micro-environment is maintained is poorly understood. The cytoarchitecture of the lateral ventricular wall with a …
Efficacy Comparison Of Various Pcr Master Mixes And Thermal Cycling Parameters For Detection Of Brucella Abortus In Multiplex Assays,
2012
California State University - Stanislaus
Efficacy Comparison Of Various Pcr Master Mixes And Thermal Cycling Parameters For Detection Of Brucella Abortus In Multiplex Assays, Kevin J. Testo, Jason A. Olivas, Alda C. Carrillo, Pejman Naraghi-Arani
STAR Program Research Presentations
Diagnostic testing for bioterrorism agents in food is essential for the safety and welfare of the general populace. Multiplex assays provide the means to test for multiple pathogen signatures with a single polymerase chain reaction (PCR) test. PCR, Luminex xMAP ® infrared fluorophore bead technology, and flow cytometry, multiplex diagnostic assays are incredibly specific and sensitive to highly conserved regions at various loci in a microbe’s genome. In order to increase the effectiveness of the PCR and the resulting fluorescent response of the beads, different PCR master mix commercial kits were compared to the standard AgPath RT-PCR mix. This study …
Nanoherbals In Human Healthcare: A Proposed Research And Development Roadmap I,
2012
Universiti Brunei Darussalam
Nanoherbals In Human Healthcare: A Proposed Research And Development Roadmap I, Ahmad S, Hashim U
ASEAN Journal on Science and Technology for Development
Bioactive secondary metabolites derived from herbs are examined for their suitability of conversion into nanoforms having flexible morphological controls, surface stabilizations and surface functionalization. This can lead to stable chemical conjugations based on molecular recognition for their possible applications in the form of smart pharmaceuticals, nutraceuticals, cosmaceuticals and many other related areas of human healthcare using green chemistry routes. Using the principles of nanoscience and technology in association with genomics and proteomics, an attempt has been made to decipher whether a suitable form of drug discovery and targeted drug delivery like schemes arefeasible in case of such nano phytochemicals using …
Nanoherbals In Human Healthcare: A Proposed Research And Development Roadmap Ii,
2012
Universiti Brunei Darussalam
Nanoherbals In Human Healthcare: A Proposed Research And Development Roadmap Ii, Ahmad S, Hashim U
ASEAN Journal on Science and Technology for Development
Bioactive secondary metabolites derived from herbs are examined for their suitability of conversion into nanoforms having flexible morphological controls, surface stabilizations and surface functionalization. This can lead to stable chemical conjugations based on molecular recognition for their possible applications in the form of smart pharmaceuticals, nutraceuticals, cosmaceuticals and many other related areas of human healthcare using green chemistry routes. Using the principles of nanoscience and technology in association with genomics and proteomics, an attempt has been made to decipher whether a suitable form of drug discovery and targeted drug delivery like schemes are feasible in case of such nano phytochemicals …
Design And Construction Of Automized Assembly Application,
2012
Universiti Brunei Darussalam
Design And Construction Of Automized Assembly Application, Htwe E.E, Hatanaka S, Akiyama T
ASEAN Journal on Science and Technology for Development
Nowadays the main stream of current automatic process and assembly is on intermittent transportation. Robot control systems can be divided into motion control and force control depending on the work. Assembly work is necessary to consider the state of force applied by detection of the force or moment on the fingers of the gripper. In this project, four groups designed and developed each one of the stages of the workpieces until the final piece was delivered; it was composed of three different workpieces. The mechanism was designed to set the shaft and fasten the screw into a vacancy in a …
Ferromagnetic Order In The Intermetallic Alloys Lani5-Xmgx,
2012
Universiti Brunei Darussalam
Ferromagnetic Order In The Intermetallic Alloys Lani5-Xmgx, Ba D.N, Tai L.T, Trung N.T, Huy N.T
ASEAN Journal on Science and Technology for Development
The influences of the substitution of Ni with Mg on crystallographic and magnetic properties of the intermetallic alloys LaNi5-xMgx (x ≤ 0.4) were investigated. The X-ray diffraction patterns showed that all samples were of single phase, and the lattice parameters, a and c, decreased slightly upon chemical doping. LaNi5 is well known as an exchange-enhanced Pauli paramagnet. Interestingly, in LaNi5-xMgx, the ferromagnetic order existed even with a small amount of dopants; the Curie temperature reached the value of room temperature for x = 0.2, and enhanced with increasing x.
Implementation Of A Communication Satellite Orbit Controller Design Using State Space Techniques,
2012
Universiti Brunei Darussalam
Implementation Of A Communication Satellite Orbit Controller Design Using State Space Techniques, Hla M.T, Lae Y.M, Kyaw S.L, Zaw M.N
ASEAN Journal on Science and Technology for Development
This research is of great importance for controlling the altitude of a satellite, especially one used for global communications in a geo-stationary orbit. The objective of this research was to advance a design based on the modelling of an orbit controller for a satellite orbiting into a circular orbit. This encompasses a good understanding of the system’s dynamics. Once a satellite is launchedinto a desired orbit, it never remains in this ideal orbit. The external forces present in space cause perturbations to the ideal orbit. To bring the satellite back into the desired orbit, on-board thrusters provide the in-orbit propulsion. …
Bond Strength Of Glued-In Rods In Malaysian Tropical Timber As Influenced By Adhesive, Diameter And Glueline Thickness,
2012
Universiti Brunei Darussalam
Bond Strength Of Glued-In Rods In Malaysian Tropical Timber As Influenced By Adhesive, Diameter And Glueline Thickness, Za'ba N.E.L, Ahmad Z, Ibrahim A
ASEAN Journal on Science and Technology for Development
Failure in wood structure is mainly caused by improper connection design, construction (fabrication) details, or serviceability. Besides using traditional bolting methods such as nails, screws and bolts to join members, timber members also can be connected by using bonded-in joints. However, current knowledge in the use of this type of timber connection is still limited. This study investigated the performance of bonded-in pultruded rods in timber as connections by exploring the effect of a few parameters such as rod type, diameter of the rod, adhesive type and thickness of the glueline. Pull-out tests were used to characterize the strength of …
Performance Evaluation Of A Uasb Reactor Using Dairy Wastes,
2012
Universiti Brunei Darussalam
Performance Evaluation Of A Uasb Reactor Using Dairy Wastes, A Ali, S Khan, K.A. Khan
ASEAN Journal on Science and Technology for Development
A UASB reactor of 6-litre capacity, made of acryl material, was operated continuously for 20–22 weeks in three different phases, using actual dairy mill effluent at a constant mesophilic temperature and neutral pH. The dairy wastewater was highly alkaline, turbid and milky in colour with a bad odour. The average COD and BOD concentrations of the dairy mill effluent were observed to be 2945 mg/l and 1070 mg/l, respectively. Phase I was the start-up phase, with OLR of 0.13 g COD/l.d. SLR, effluent VFAs, effluent TSS, biogas production and methane composition observed during this phase were 1.4 g COD/g VSS.d, …
Ethanol And Anaerobic Conditions Reversibly Inhibit Commercial Cellulase Activity In Thermophilic Simultaneous Saccharification And Fermentation (Tssf),
2012
Dartmouth College
Ethanol And Anaerobic Conditions Reversibly Inhibit Commercial Cellulase Activity In Thermophilic Simultaneous Saccharification And Fermentation (Tssf), Kara K. Podkaminer, William R. Kenealy, Christopher D. Herring, David A. Hogsett, Lee R. Lynd
Dartmouth Scholarship
A previously developed mathematical model of low solids thermophilic simultaneous saccharification and fermentation (tSSF) with Avicel was unable to predict performance at high solids using a commercial cellulase preparation (Spezyme CP) and the high ethanol yield Thermoanaerobacterium saccharolyticum strain ALK2. The observed hydrolysis proceeded more slowly than predicted at solids concentrations greater than 50 g/L Avicel. Factors responsible for this inaccuracy were investigated in this study.
Enhanced Hydrocarbon Biodegradation Using Bioaugmentation With Biowish™-Aqua Fog,
2012
California Polytechnic State University, San Luis Obispo
Enhanced Hydrocarbon Biodegradation Using Bioaugmentation With Biowish™-Aqua Fog, Michael Robert Lehrer
Master's Theses
This study was done to determine the effectiveness of a commercially available bioaugmentation product, BiOWiSHTM-Aqua FOG, for remediating petroleum-contaminated sandy soil. Biodegradation enhancement by BiOWiSHTM-Aqua FOG was evaluated in laboratory microcosms by directly measuring total petroleum hydrocarbon (TPH) and indirectly using respirometry. Attempts were made to enrich hydrocarbon-degrading bacteria in BiOWiSHTM-Aqua FOG, and the resulting enrichment cultures were screened using respirometry as well. Potential hydrocarbon-degrading bacteria in BiOWiSHTM-Aqua FOG were isolated. Experiments were performed at bench-scale using microcosm bottles containing sand contaminated with either motor oil or No. 2 diesel fuel. The …
Urinary Copper Elevation In A Mouse Model Of Wilson’S
Disease Is A Regulated Process To Specifically Decrease
The Hepatic Copper Load,
2012
Johns Hopkins University
Urinary Copper Elevation In A Mouse Model Of Wilson’S Disease Is A Regulated Process To Specifically Decrease The Hepatic Copper Load, Lawrence W. Gray, Fangyu Peng, Shannon A. Molloy, Venkata S. Pendyala, Abigael Muchenditsi, Otto Muzik, Jaekwon Lee, Jack H. Kaplan, Svetlana Lutsenko
Department of Biochemistry: Faculty Publications
Body copper homeostasis is regulated by the liver, which removes excess copper via bile. In Wilson’s disease (WD), this function is disrupted due to inactivation of the copper transporter ATP7B resulting in hepatic copper overload. High urinary copper is a diagnostic feature of WD linked to liver malfunction; the mechanism behind urinary copper elevation is not fully understood. Using Positron Emission Tomography-Computed Tomography (PET-CT) imaging of live Atp7b‒/‒ mice at different stages of disease, a longitudinal metal analysis, and characterization of copper-binding molecules, we show that urinary copper elevation is a specific regulatory process mediated …
Energy-Saving Hypothermia Reduces Flight Ability In Mourning Doves,
2012
Indiana State University
Energy-Saving Hypothermia Reduces Flight Ability In Mourning Doves, Jennie M. Carr, Steven L. Lima
Symposium 2012
Overwintering birds are frequently exposed to thermal challenges that can quickly decrease energy reserves, thus leading to an increase in the risk of starvation. During these periods of energetic hardship, many avian species use nocturnal hypothermia to conserve energy that would otherwise be lost remaining warm throughout the night (McKechnie & Lovegrove 2002). However, a cool body temperature (Tb) may limit a bird’s ability to monitor the environment and may slow their response to a potential threat. Thus, birds likely trade-off between the benefits of energy-saving hypothermia and the potential costs of reduced behavioral responsiveness to predators (Welton et al. …
Conservation Of Oxidative Protein Stabilization In An Insect
Homologue Of The Parkinsonism-Associated Protein Dj-1,
2012
University of Nebraska-Lincoln
Conservation Of Oxidative Protein Stabilization In An Insect Homologue Of The Parkinsonism-Associated Protein Dj-1, Jiusheng Lin, Janani Prahlad, Mark A. Wilson
Department of Biochemistry: Faculty Publications
DJ-1 is a conserved, disease-associated protein that protects against oxidative stress and mitochondrial damage in multiple organisms. Human DJ-1 contains a functionally essential cysteine residue (Cys106) whose oxidation is important for regulating protein function by an unknown mechanism. This residue is well conserved in other DJ-1 homologues, including two (DJ-1α and DJ-1β) in Drosophila melanogaster. Because D. melanogaster is a powerful model system for studying DJ-1 function, we have determined the crystal structure and impact of cysteine oxidation on Drosophila DJ-1β. The structure of D. melanogaster DJ-1β is similar to that of human DJ-1, although two important residues in …
Dcm Methylation Is Detrimental To Plasmid Transformation In Clostridium Thermocellum,
2012
Dartmouth College
Dcm Methylation Is Detrimental To Plasmid Transformation In Clostridium Thermocellum, Adam M. Guss, Daniel G. Olson, Nicky C. Caiazza, Lee R. Lynd
Dartmouth Scholarship
Background: Industrial production of biofuels and other products by cellulolytic microorganisms is of interest but hindered by the nascent state of genetic tools. Although a genetic system for Clostridium thermocellum DSM1313 has recently been developed, available methods achieve relatively low efficiency and similar plasmids can transform C. thermocellum at dramatically different efficiencies. Results: We report an increase in transformation efficiency of C. thermocellum for a variety of plasmids by using DNA that has been methylated by Escherichia coli Dam but not Dcm methylases. When isolated from a dam + dcm + E. coli strain, pAMG206 transforms C. thermocellum 100-fold better …
Design And Implementation Of A Shoulder Simulator,
2012
Syracuse University
Design And Implementation Of A Shoulder Simulator, Chelsea Lynn Kowal
Renée Crown University Honors Thesis Projects - All
The main objective of this thesis project is to build a shoulder simulator to be able to understand the mechanics of the shoulder, specifically the rotator cuff and deltoid muscles. The shoulder simulator should be able to mimic the important motions of the human shoulder by pulling on tendons of cadaver shoulders and moving the shoulder in various specified motions. In my thesis project, I worked on creating a shoulder simulator that would control the rotator cuff muscles along with the three deltoid muscles. In order to do this, I needed to design and test the shoulder simulator using cadaver …
Biomass Assisted Synthesis Of Antibacterial Gold Nanoparticles And Commentary On Its Future Potential And Applications In Medicine,
2012
Western Kentucky University
Biomass Assisted Synthesis Of Antibacterial Gold Nanoparticles And Commentary On Its Future Potential And Applications In Medicine, Chad Willis
Mahurin Honors College Capstone Experience/Thesis Projects
Xylose is a natural monosaccharide found in biomass such as straw, pecan shells, cottonseed hulls, and corncobs. Using this monosaccharide, we report the green synthesis and characterization of biocompatible, biodegradable xylose encapsulated gold nanoparticles (Xyl-GNPs) with potential antibacterial activity. GNPs were synthesized using the bioreduction property of xylose on the chloroaurate anions in an aqueous solution at room temperature and at atmospheric pressure. The characterization of synthesized GNPs was examined by UV-vis spectroscopy; transmission electron microscopy (TEM), energy-dispersive x-ray spectroscopy (EDS) and fourier transform infrared spectroscopy (FTIR). Results indicate that the particles were stable; near spherical in shape with an …
Fluorescence-Based Reporter For Gauging Cyclic Di-Gmp Levels In Pseudomonas Aeruginosa,
2012
University of Copenhagen
Fluorescence-Based Reporter For Gauging Cyclic Di-Gmp Levels In Pseudomonas Aeruginosa, Morten T. Rybtke, Bradley R. Borlee, Keiji Murakami, Yasuhiko Irie, Morten Hentzer, Thomas E. Nielsen, Michael Givskov, Matthew R. Parsek, Tim Tolker-Nielsen
Biology Faculty Publications
The increased tolerance toward the host immune system and antibiotics displayed by biofilm-forming Pseudomonas aeruginosa and other bacteria in chronic infections such as cystic fibrosis bronchopneumonia is of major concern. Targeting of biofilm formation is believed to be a key aspect in the development of novel antipathogenic drugs that can augment the effect of classic antibiotics by decreasing antimicrobial tolerance. The second messenger cyclic di-GMP is a positive regulator of biofilm formation, and cyclic di-GMP signaling is now regarded as a potential target for the development of antipathogenic compounds. Here we describe the development of fluorescent monitors that can gauge …
Automation Of Tangential Flow Filtration For Purification Of Biosynthetic Spider Silk,
2012
Utah State University
Automation Of Tangential Flow Filtration For Purification Of Biosynthetic Spider Silk, Candace N. Clark
Undergraduate Honors Capstone Projects
The objective of this project is to create a user-friendly, automated flow regulation system for a tangential flow filtration (TFF) process. These filtration units are crucial for the initial stages of extracting the spider silk proteins from transgenic goat milk. Automated flow regulation systems do exist, but not at an appropriate scale for this application. This system will allow bench-top studies to be conducted with automated efficiency. Spider silk is an amazing material and if commercially available, it could be used in hundreds of applications. Currently, cost effective production is the most significant issue stopping large-scale production and use of …
