An Active Efficient Coding Model Of The Development Of Amblyopia, 2018 Frankfurt Institute for Advanced Studies
An Active Efficient Coding Model Of The Development Of Amblyopia, Samuel Eckmann, Lukas Klimmasch, Bertram Shi, Jochen Triesch
No abstract provided.
Differential Diagnosis Of Pan-Uveitis: Behçet’S Disease, 2018 East Tennessee State University
Differential Diagnosis Of Pan-Uveitis: Behçet’S Disease, Peter Blosser, Remil Simon, Courtney Ridner
Appalachian Student Research Forum
This report describes the case of a 56-year-old man who presented with blurry vision, increased intraocular pressure, and conjunctival injection after posterior chamber intraocular lens implantation. Initially post-operative endophalmitis and foreign body inflammation were considered as differential diagnoses, but after further examination pan-uveitis was diagnosed. Uveitis is an ocular finding that may indicate several diseases, one of which is Behçet’s Disease. During the interview, the patient mentioned a history of apthous ulcers and genital ulcers which then lead to the clinical diagnosis of Behçet’s Disease. This report emphasizes that Behçet’s Disease is rare in Caucasians. Therefore, is ...
Reduced Intraepithelial Corneal Nerve Density And Sensitivity Accompany Desiccating Stress And Aging In C57bl/6 Mice, 2018 George Washington University
Reduced Intraepithelial Corneal Nerve Density And Sensitivity Accompany Desiccating Stress And Aging In C57bl/6 Mice, Mary Ann Stepp, Sonali Pal-Ghosh, Gauri Tadvalkar, Alexa Williams, S Pflugfelder, C De Paiva
Anatomy and Regenerative Biology Faculty Publications
Dry Eye disease causes discomfort and pain in millions of patients. Using a mouse acute desiccating stress (DS) model we show that DS induces a reduction in intraepithelial corneal nerve (ICN) density, corneal sensitivity, and apical extension of the intraepithelial nerve terminals (INTs) that branch from the subbasal nerves (SBNs). Topical application of 0.02% Mitomycin C (MMC) or vehicle alone has no impact on the overall loss of axon density due to acute DS. Chronic dry eye, which develops progressively as C57BL/6 mice age, is accompanied by significant loss of the ICNs and corneal sensitivity between 2 and ...
Transcriptome Profiling Of Neovascularized Corneas Reveals Mir-204 As A Multi-Target Biotherapy Deliverable By Raavs, 2018 University of Massachusetts Medical School
Transcriptome Profiling Of Neovascularized Corneas Reveals Mir-204 As A Multi-Target Biotherapy Deliverable By Raavs, Yi Lu, Phillip W. L. Tai, Jianzhong Ai, Dominic J. Gessler, Qin Su, Xieyi Yao, Qiang Zheng, Phillip D. Zamore, Xun Xu, Guangping Gao
Open Access Articles
Corneal neovascularization (NV) is the major sight-threatening pathology caused by angiogenic stimuli. Current drugs that directly target pro-angiogenic factors to inhibit or reverse the disease require multiple rounds of administration and have limited efficacies. Here, we identify potential anti-angiogenic corneal microRNAs (miRNAs) and demonstrate a framework that employs discovered miRNAs as biotherapies deliverable by recombinant adeno-associated viruses (rAAVs). By querying differentially expressed miRNAs in neovascularized mouse corneas induced by alkali burn, we have revealed 39 miRNAs that are predicted to target more than 5,500 differentially expressed corneal mRNAs. Among these, we selected miR-204 and assessed its efficacy and therapeutic ...
Slt Versus Alt In Glaucoma Patients Treated Previously With 360-Degree Slt—A Randomised, Single-Blind, Equivalence Clinical Trial, 2018 The University of Western Ontario
Slt Versus Alt In Glaucoma Patients Treated Previously With 360-Degree Slt—A Randomised, Single-Blind, Equivalence Clinical Trial, Hui Guo
Electronic Thesis and Dissertation Repository
Our objectives were to compare the efficacy and safety between selective laser trabeculoplasty (SLT) and argon laser trabeculoplasty (ALT) for the open-angle glaucoma patients who had 360-degree SLT previously and to explore the baseline predictors for the treatment success. Data were obtained from a multicenter, patient-masked, randomised, parallel-group, active-controlled trial. The enrolled patients were randomly assigned to receive either SLT or ALT. At 12-month follow-up, the mean intraocular pressure (IOP) reduction was 3.35 mmHg and 3.36 mmHg after SLT and ALT respectively. The difference of mean IOP reduction was -0.01 mmHg (n=115). The 95% CI of ...
Dznep Inhibits H3k27me3 Deposition And Delays Retinal Degeneration In The Rd1 Mice, 2018 Sichuan University
Dznep Inhibits H3k27me3 Deposition And Delays Retinal Degeneration In The Rd1 Mice, Shijie Zheng, Lirong Xiao, Yu Liu, Yujiao Wang, Lin Cheng, Junjun Zhang, Naihong Yan, Danian Chen
Open Access Articles
Retinitis pigmentosa (RP) is a group of inherited retinal degenerative diseases causing progressive loss of photoreceptors. Numerous gene mutations are identified to be related with RP, but epigenetic modifications may also be involved in the pathogenesis. Previous studies suggested that both DNA methylation and histone acetylation regulate photoreceptor cell death in RP mouse models. However, the role of histone methylation in RP has never been investigated. In this study, we found that trimethylation of several lysine sites of histone H3, including lysine 27 (H3K27me3), increased in the retinas of rd1 mice. Histone methylation inhibitor DZNep significantly reduced the calpain activity ...
Treatment Challenges In An Atypical Presentation Of Tubulointerstitial Nephritis And Uveitis (Tinu), 2018 George Washington University
Treatment Challenges In An Atypical Presentation Of Tubulointerstitial Nephritis And Uveitis (Tinu), S Caplash, S Gangaputra, S Kodati, Shamir Tuchman, Hemalatha Srinivasalu, H Sen
Pediatrics Faculty Publications
Purpose: To describe an atypical presentation of Tubulointerstitial Nephritis and Uveitis (TINU), with challenges in treatment course. Observations: A 12-year-old Hispanic female presented to the National Eye Institute's Uveitis clinic with bilateral blurred vision, red eyes and photophobia, not responsive to topical steroids. On exam, she had bilateral severe panuveitis with areas of subretinal fluid. During her evaluation, she was noted to have elevated serum creatinine. A kidney biopsy confirmed the presence of severe tubulointerstitial nephritis and interstitial fibrosis. She was treated with oral steroids with excellent resolution of symptoms and subretinal fluid. She continued to have anterior segment ...
Analysis Of Ocular Changes In A Cyp1b1-/- Mouse, 2018 The University of Akron
Analysis Of Ocular Changes In A Cyp1b1-/- Mouse, Kylie Barto
Honors Research Projects
The goal of the research is to investigate the structural changes seen in Primary Congenital Glaucoma using a mouse model. Eye sections of a Cyp1b1-/- mouse were compared to eye sections of a healthy normal mouse (wild type, WT). Initial examination of the cornea, iris, and irodocorneal angle showed structural changes in Cyp1b1-/-. These changes were similar to those described in the literature using other animal models. This suggests that a mutation in Cyp1b1-/- in a mouse results in a phenotype similar to that seen in PCG human patients and this model could be used to investigate the pathophysiology of ...
Gene Therapy Using A Minicep290 Fragment Delays Photoreceptor Degeneration In A Mouse Model Of Leber Congenital Amaurosis, 2018 University of Massachusetts Medical School
Gene Therapy Using A Minicep290 Fragment Delays Photoreceptor Degeneration In A Mouse Model Of Leber Congenital Amaurosis, Wei Zhang, Linjing Li, Qin Su, Guangping Gao, Hemant Khanna
Open Access Articles
Mutations in the cilia-centrosomal protein CEP290 are frequently observed in autosomal recessive childhood blindness disorder Leber congenital amaurosis (LCA). No treatment or cure currently exists for this disorder. The Cep290(rd16) (retinal degeneration 16) mouse (a model of LCA) carries a mutation in the Cep290 gene. This mutation leads to shorter cilia formation and defective photoreceptor structure and function. A roadblock to developing a gene replacement strategy for CEP290 using conventional adeno-associated virus (AAV) vectors is its large size. The identification and characterization is reported of a miniCEP290 gene that is amenable to AAV2/8-mediated delivery and delaying retinal degeneration ...
Loss Of The Cone-Enriched Caspase-7 Does Not Affect Secondary Cone Death In Retinitis Pigmentosa, 2017 University of Massachusetts Medical School
Loss Of The Cone-Enriched Caspase-7 Does Not Affect Secondary Cone Death In Retinitis Pigmentosa, Aditya Venkatesh, Shun-Yun Cheng, Claudio Punzo
University of Massachusetts Medical School Faculty Publications
Purpose: The apoptotic mechanisms responsible for secondary cone death in retinitis pigmentosa (RP) remain largely unknown. The cone-enriched apoptotic protease caspase-7 (Casp7) is thought to be triggered by endoplasmic reticulum (ER) stress and plays a pivotal role in mice deficient in the cone cyclic nucleotide-gated channels, a deficiency that causes achromatopsia in humans and in mice with autosomal dominant rhodopsin mutations, in particular the T17M mutation. Thus, we tested in two mouse models of RP whether the cone-enriched Casp7 plays a role during secondary cone death.
Methods: Casp7 knockout mice were crossed to two different RP mouse models with significantly ...
Modifiable And Non-Modifiable Factors Related To The Macular Pigment Optical Density (Mpod) In A Population Of College-Aged Adults, 2017 Louisiana State University and Agricultural and Mechanical College
Modifiable And Non-Modifiable Factors Related To The Macular Pigment Optical Density (Mpod) In A Population Of College-Aged Adults, Genesis Gisselle Guerra Gaitan
LSU Master's Theses
Age-related macular degeneration (AMD) is one of the leading causes of blindness among the elderly worldwide. Retinal problems, specifically in the macula, hold potential for development of AMD. Macular pigment optical density (MPOD) gives a measure of the thickness of the macula and therefore health of the macula. Some of the populations at risk for development of AMD include being white, female, and having light eye color. Factors related to the development of the disease have been divided into modifiable and non-modifiable; non-modifiable include a genetic predisposition. Control of modifiable factors, including body mass index (BMI) and diet [dietary intake ...
Gelsolin Dysfunction Causes Photoreceptor Loss In Induced Pluripotent Cell And Animal Retinitis Pigmentosa Models, 2017 University of Edinburgh
Gelsolin Dysfunction Causes Photoreceptor Loss In Induced Pluripotent Cell And Animal Retinitis Pigmentosa Models, Roly Megaw, Hashem Abu-Arafeh, Melissa Jungnickel, Carla Mellough, Christine Gurniak, Walter Witke, Wei Zhang, Hemant Khanna, Pleasantine Mill, Baljean Dhillon, Alan F. Wright, Majlinda Lako, Charles Ffrench-Constant
Open Access Articles
Mutations in the Retinitis Pigmentosa GTPase Regulator (RPGR) cause X-linked RP (XLRP), an untreatable, inherited retinal dystrophy that leads to premature blindness. RPGR localises to the photoreceptor connecting cilium where its function remains unknown. Here we show, using murine and human induced pluripotent stem cell models, that RPGR interacts with and activates the actin-severing protein gelsolin, and that gelsolin regulates actin disassembly in the connecting cilium, thus facilitating rhodopsin transport to photoreceptor outer segments. Disease-causing RPGR mutations perturb this RPGR-gelsolin interaction, compromising gelsolin activation. Both RPGR and Gelsolin knockout mice show abnormalities of actin polymerisation and mislocalisation of rhodopsin in ...
Moxifloxacin Concentration And Proteomic Analysis Of Aqueous Humor In Human Uveitis Associated With Oral Moxifloxacin Therapy, 2017 University of Massachusetts Medical School
Moxifloxacin Concentration And Proteomic Analysis Of Aqueous Humor In Human Uveitis Associated With Oral Moxifloxacin Therapy, David M. Hinkle, Nicole A. Kruh-Garcia, Jonathan N. Kruh, Carolyn Broccardo, Priyanka Doctor, C. Stephen Foster
Open Access Articles
PURPOSE: The aim was to report the aqueous humor moxifloxacin concentration and proteome profile of an individual with bilateral uveitis-like syndrome with pigment dispersion.
METHODS: Multiple reactions monitoring mass spectrometry quantified the aqueous concentration of moxifloxacin in the affected individual. Shotgun proteomic analysis performed via liquid chromatography tandem mass spectrometry (LC-MS/MS) defined the protein profile in the affected individual and unaffected control samples.
RESULTS: Moxifloxacin was present at higher than expected levels in aqueous humor 18 days following oral administration. One-third of the proteins were identified by significantly lower spectral counts in the aqueous of the individual with moxifloxacin ...
Corneal Edema And Keratitis Following Selective Laser Trabeculoplasty, 2017 George Washington University
Corneal Edema And Keratitis Following Selective Laser Trabeculoplasty, E T. Liu, L S. Seery, A Arosemena, Tania Lamba, C J. Chaya
Ophthalmology Faculty Publications
To describe three cases of keratitis following Selective Laser Trabeculoplasty(SLT).
Three females with a history of glaucoma presented with corneal edema, keratitis (endothelial, epithelial) and decreased visual acuity shortly after SLT. There was variable resolution of symptoms after starting treatment with oral antiherpetics and topical steroids.
Conclusions and importance
With the increase in usage of SLT as a treatment for glaucoma and subsequent reports of keratitis, it is imperative for ophthalmic surgeons to be aware of herpes simplex as a possible risk factor. Prompt treatment with antivirals and steroids can potentially prevent scarring and permanent damage to ...
Retinoblastoma Tells The Story Of Our Health Care System, 2017 Aga Khan University
Retinoblastoma Tells The Story Of Our Health Care System, Irshad Soomro, Muhammad Nadeem Khan, Suhail Muzaffar, Naila Kayani, Shahid Pervez, Akbar Shah Hussainy, Rashida Ahmed, Sheema H. Hasan
Objective: To review cases of retinoblastoma. Setting: Department of Pathology Aga Khan University Hospital Karachi. Method: Twenty three specimens from cases of retinoblastoma received over a period of eight years were routinely processes and stained with haematoxylin and Eosin stain Other stains were used for tuberculosis and melanin. Immunochemistry was resorted to in undifferentiated tumors. Results: Over 60% cases of retinoblastoma were diagnosed after 5 years and nine cases showed involvement of opti-nerve. Conclusion: Late diagnosis of retinoblastoma effects the stage of the tumors and the prognisis.
Atorvastatin Promotes Phagocytosis And Attenuates Pro-Inflammatory Response In Human Retinal Pigment Epithelial Cells, Bo Tian, Ahmad Al-Moujahed, Peggy Bouzika, Yijun Hu, Shoji Notomi, Pavlina Tsoka, Joan W. Miller, Haijiang Lin, Demetrios G. Vavvas
Open Access Articles
Phagocytosis of daily shed photoreceptor outer segments is an important function of the retinal pigment epithelium (RPE) and it is essential for retinal homeostasis. RPE dysfunction, especially impairment of its phagocytic ability, plays an essential role in the pathogenesis of age-related macular degeneration (AMD). Statins, or HMG CoA (3-hydroxy-3-methylglutaryl-coenzyme A) reductase inhibitors, are drugs with multiple properties that have been extensively used to treat hyperlipidemia. However, their effect on RPE cells has not been fully elucidated. Here we report that high dose atorvastatin increased the phagocytic function of ARPE-19 cells, as well as rescue the cells from the phagocytic dysfunction ...
Interaction Of The Tear Film With The Ocular Surface, 2017 University of Delaware
Interaction Of The Tear Film With The Ocular Surface, Richard J. Braun, Amy Janett, Nicholas Gewecke, Spencer Walker
Biology and Medicine Through Mathematics Conference
No abstract provided.
Quantitative Microvascular Analysis Of Retinal Venous Occlusions By Spectral Domain Optical Coherence Tomography Angiography, 2017 University of Massachusetts Medical School
Quantitative Microvascular Analysis Of Retinal Venous Occlusions By Spectral Domain Optical Coherence Tomography Angiography, Nicole Koulisis, Alice Y. Kim, Zhongdi Chu, Anoush Shahidzadeh, Bruce Burkemper, Lisa C. Olmos De Koo, Andrew A. Moshfeghi, Hossein Ameri, Carmen A. Puliafito, Veronica L. Isozaki, Ruikang K. Wang, Amir H. Kashani
Open Access Articles
PURPOSE: To quantitatively evaluate the retinal microvasculature in human subjects with retinal venous occlusions (RVO) using optical coherence tomography angiography (OCTA). DESIGN: Retrospective, cross-sectional, observational case series. PARTICIPANTS: Sixty subjects (84 eyes) were included (20 BRVO, 14 CRVO, 24 unaffected fellow eyes, and 26 controls). METHODS: OCTA was performed on a prototype, spectral domain-OCTA system in the 3x3mm central macular region. Custom software was used to quantify morphology and density of retinal capillaries using four quantitative parameters. The vasculature of the segmented retinal layers and nonsegmented whole retina were analyzed. MAIN OUTCOME MEASURES: Fractal dimension (FD), vessel density (VD), skeletal ...
Efficacy Of Selective Laser Trabeculoplasty In Patients With Open-Angle Glaucoma Or Ocular Hypertension: A Systematic Review And Meta-Analysis, 2017 The University of Western Ontario
Efficacy Of Selective Laser Trabeculoplasty In Patients With Open-Angle Glaucoma Or Ocular Hypertension: A Systematic Review And Meta-Analysis, Muna Hassan
Electronic Thesis and Dissertation Repository
Purpose: To determine the efficacy of selective laser trabeculoplasty (SLT) in lowering intra-ocular pressure (IOP) levels and reducing the number of medications in patients with open angle glaucoma (OAG) or ocular hypertension (OHT).
Methods: A systematic review was conducted by searching various databases including MEDLINE (Ovid), EMBASE (Ovid), CINAHL, Cochrane Library, Web of Science- Core Collections, BIOSIS Previews, and Scopus. Duplicates were removed and articles were screened using EPPI Reviewer 4.0. A meta-analysis was conducted using STATA 13.0. Weighted mean difference (WMD) was computed and the heterogeneity statistic was assessed using the I2. Fixed and random effects ...
Latanoprost And Timolol In Hydrogel Soft Drug-Dispensing Contact Lenses: A Comparative Analysis, 2017 Virginia Commonwealth University
Latanoprost And Timolol In Hydrogel Soft Drug-Dispensing Contact Lenses: A Comparative Analysis, Neha Potdar
Virginias Collegiate Honors Council Conference
Glaucoma patients only can instill glaucoma eye drops. This results in 1-7% absorption of the medication. Surveys show that patients forget to take their medication and have difficulty inserting their eye drops. Researchers at the Massachusetts Eye and Ear Infirmary have developed latanoprost drug-dispensing hydrogel soft contact lenses that dispense the drug to help facilitate the process. However, some glaucoma patients do not respond to latanoprost because they have uveitic glaucoma or do not want to experience latanoprost’s ocular side effects. Patients need an alternative which can be inserted into these contact lenses. Another commonly used glaucoma drug is ...