Moxifloxacin Concentration And Proteomic Analysis Of Aqueous Humor In Human Uveitis Associated With Oral Moxifloxacin Therapy, 2017 University of Massachusetts Medical School
Moxifloxacin Concentration And Proteomic Analysis Of Aqueous Humor In Human Uveitis Associated With Oral Moxifloxacin Therapy, David M. Hinkle, Nicole A. Kruh-Garcia, Jonathan N. Kruh, Carolyn Broccardo, Priyanka Doctor, C. Stephen Foster
Open Access Articles
PURPOSE: The aim was to report the aqueous humor moxifloxacin concentration and proteome profile of an individual with bilateral uveitis-like syndrome with pigment dispersion.
METHODS: Multiple reactions monitoring mass spectrometry quantified the aqueous concentration of moxifloxacin in the affected individual. Shotgun proteomic analysis performed via liquid chromatography tandem mass spectrometry (LC-MS/MS) defined the protein profile in the affected individual and unaffected control samples.
RESULTS: Moxifloxacin was present at higher than expected levels in aqueous humor 18 days following oral administration. One-third of the proteins were identified by significantly lower spectral counts in the aqueous of the individual with moxifloxacin ...
Retinoblastoma Tells The Story Of Our Health Care System, 2017 Aga Khan University
Retinoblastoma Tells The Story Of Our Health Care System, Irshad Soomro, Muhammad Nadeem Khan, Suhail Muzaffar, Naila Kayani, Shahid Pervez, Akbar Shah Hussainy, Rashida Ahmed, Sheema H. Hasan
Objective: To review cases of retinoblastoma. Setting: Department of Pathology Aga Khan University Hospital Karachi. Method: Twenty three specimens from cases of retinoblastoma received over a period of eight years were routinely processes and stained with haematoxylin and Eosin stain Other stains were used for tuberculosis and melanin. Immunochemistry was resorted to in undifferentiated tumors. Results: Over 60% cases of retinoblastoma were diagnosed after 5 years and nine cases showed involvement of opti-nerve. Conclusion: Late diagnosis of retinoblastoma effects the stage of the tumors and the prognisis.
Atorvastatin Promotes Phagocytosis And Attenuates Pro-Inflammatory Response In Human Retinal Pigment Epithelial Cells, Bo Tian, Ahmad Al-Moujahed, Peggy Bouzika, Yijun Hu, Shoji Notomi, Pavlina Tsoka, Joan W. Miller, Haijiang Lin, Demetrios G. Vavvas
Open Access Articles
Phagocytosis of daily shed photoreceptor outer segments is an important function of the retinal pigment epithelium (RPE) and it is essential for retinal homeostasis. RPE dysfunction, especially impairment of its phagocytic ability, plays an essential role in the pathogenesis of age-related macular degeneration (AMD). Statins, or HMG CoA (3-hydroxy-3-methylglutaryl-coenzyme A) reductase inhibitors, are drugs with multiple properties that have been extensively used to treat hyperlipidemia. However, their effect on RPE cells has not been fully elucidated. Here we report that high dose atorvastatin increased the phagocytic function of ARPE-19 cells, as well as rescue the cells from the phagocytic dysfunction ...
Interaction Of The Tear Film With The Ocular Surface, 2017 University of Delaware
Interaction Of The Tear Film With The Ocular Surface, Richard J. Braun, Amy Janett, Nicholas Gewecke, Spencer Walker
Biology and Medicine Through Mathematics Conference
No abstract provided.
Quantitative Microvascular Analysis Of Retinal Venous Occlusions By Spectral Domain Optical Coherence Tomography Angiography, 2017 University of Massachusetts Medical School
Quantitative Microvascular Analysis Of Retinal Venous Occlusions By Spectral Domain Optical Coherence Tomography Angiography, Nicole Koulisis, Alice Y. Kim, Zhongdi Chu, Anoush Shahidzadeh, Bruce Burkemper, Lisa C. Olmos De Koo, Andrew A. Moshfeghi, Hossein Ameri, Carmen A. Puliafito, Veronica L. Isozaki, Ruikang K. Wang, Amir H. Kashani
Open Access Articles
PURPOSE: To quantitatively evaluate the retinal microvasculature in human subjects with retinal venous occlusions (RVO) using optical coherence tomography angiography (OCTA). DESIGN: Retrospective, cross-sectional, observational case series. PARTICIPANTS: Sixty subjects (84 eyes) were included (20 BRVO, 14 CRVO, 24 unaffected fellow eyes, and 26 controls). METHODS: OCTA was performed on a prototype, spectral domain-OCTA system in the 3x3mm central macular region. Custom software was used to quantify morphology and density of retinal capillaries using four quantitative parameters. The vasculature of the segmented retinal layers and nonsegmented whole retina were analyzed. MAIN OUTCOME MEASURES: Fractal dimension (FD), vessel density (VD), skeletal ...
Latanoprost And Timolol In Hydrogel Soft Drug-Dispensing Contact Lenses: A Comparative Analysis, 2017 Virginia Commonwealth University
Latanoprost And Timolol In Hydrogel Soft Drug-Dispensing Contact Lenses: A Comparative Analysis, Neha Potdar
Virginias Collegiate Honors Council Conference
Glaucoma patients only can instill glaucoma eye drops. This results in 1-7% absorption of the medication. Surveys show that patients forget to take their medication and have difficulty inserting their eye drops. Researchers at the Massachusetts Eye and Ear Infirmary have developed latanoprost drug-dispensing hydrogel soft contact lenses that dispense the drug to help facilitate the process. However, some glaucoma patients do not respond to latanoprost because they have uveitic glaucoma or do not want to experience latanoprost’s ocular side effects. Patients need an alternative which can be inserted into these contact lenses. Another commonly used glaucoma drug is ...
Inhibition Or Stimulation Of Autophagy Affects Early Formation Of Lipofuscin-Like Autofluorescence In The Retinal Pigment Epithelium Cell, 2017 Sun Yat-sen University, China
Inhibition Or Stimulation Of Autophagy Affects Early Formation Of Lipofuscin-Like Autofluorescence In The Retinal Pigment Epithelium Cell, Lei Lei, Radouil T. Tzekov, Huapeng Li, J. Hugh Mcdowell, Guangping Gao, W. Clay Smith, Shibo Tang, Shalesh Kaushal
Open Access Articles
The accumulation of lipofuscin in the retinal pigment epithelium (RPE) is dependent on the effectiveness of photoreceptor outer segment material degradation. This study explored the role of autophagy in the fate of RPE lipofuscin degradation. After seven days of feeding with either native or modified rod outer segments, ARPE-19 cells were treated with enhancers or inhibitors of autophagy and the autofluorescence was detected by fluorescence-activated cell sorting. Supplementation with different types of rod outer segments increased lipofuscin-like autofluorescence (LLAF) after the inhibition of autophagy, while the induction of autophagy (e.g., application of rapamycin) decreased LLAF. The effects of autophagy ...
Macular Pigment Is Associated With Glare-Affected Visual Function And Central Visual Field Loss In Glaucoma, 2017 Dublin Institute of Technology
Macular Pigment Is Associated With Glare-Affected Visual Function And Central Visual Field Loss In Glaucoma, James Loughman, We Fong Siah, Colm O’Brien
To evaluate the relationship between macular pigment optical density (MPOD) and glare disability in open-angle glaucoma.
Methods A cross-sectional analysis of baseline data (88 subjects; median age, 67 (range 36–84) years) collected during the Macular Pigment and Glaucoma Trial (ISRCTN registry number: 56985060). MPOD at 0.25°, 0.5° and 1° of retinal eccentricity was measured using customised heterochromatic flicker photometry. Mesopic contrast sensitivity with glare (mCSg), photostress recovery time (PRT) and self-reported glare symptoms were evaluated. Fourier-domain optical coherence tomography was used to analyse ganglion cell complex (GCC) and identify foveal involvement.
Results Low spatial frequency (f ...
Antibiotic Prophylaxis In Orbital Fractures., 2017 George Washington University
Antibiotic Prophylaxis In Orbital Fractures., Benjamin Reiss, Lamise Rajjoub, Tamer Mansour, Tony Chen, Aisha Mumtaz
Ophthalmology Faculty Publications
To determine whether prophylactic antibiotic use in patients with orbital fracture prevent orbital infection.
Retrospective cohort study.
All patients diagnosed with orbital fracture between January 1, 2008 and March 1, 2014 at The George Washington University Hospital and Clinics.
Main Outcome Measures:
Development of orbital infection.
One hundred seventy-two patients with orbital fracture met our inclusion and exclusion criteria. No orbital infections were documented. Twenty subjects (12%) received no prophylactic antibiotic, and two (1%) received only one dose of antibiotics pre-operatively for surgery. For primary antibiotic, 136 subjects (79%) received oral antibiotics, and 14 (8%) received ...
Why Is There An Association Between Retinal Vein Occlusion, Vision Loss, Myocardial Infarction, Stroke And Mortality: Potential Roles Of Hypomagnesaemia, Release Of Sphingolipids, And Platelet-Activating Factor, 2017 Touro College of Osteopathic Medicine (Middletown)
Why Is There An Association Between Retinal Vein Occlusion, Vision Loss, Myocardial Infarction, Stroke And Mortality: Potential Roles Of Hypomagnesaemia, Release Of Sphingolipids, And Platelet-Activating Factor, Burton M. Altura, Asefa Gebrewold, Nilank C. Shah, Aimin Zhang, Wenyan Li, Lawrence M. Resnick, Bella T. Altura
Touro College of Osteopathic Medicine (Middletown) Publications and Research
Although numerous hypotheses (and potential risk factors), have been offered to explain the origins and potential mechanism(s) for central retinal vein occlusion (RVO) in patients of diverse ages, there is no agreement. Recently, considerable epidemiological evidence has been brought forth which indicates a strong association between RVO, myocardial infarctions, heart failure, and stroke. Magnesium (Mg) deficiency (mgD) has long-been associated with glaucoma and diabetic retinopathy. Over the past three decades, our laboratories have found strong associations of mgD linked to morbidity/ mortality in cardiovascular diseases such as myocardial infarctions, cardiac failure, atherogenesis, and strokes, both experimentally and clinically. More ...
Are Contact Lenses An Effective Vehicle For Ocular-Disease Drug Delivery?, 2017 Touro College
Are Contact Lenses An Effective Vehicle For Ocular-Disease Drug Delivery?, Chana Haimowitz
The Science Journal of the Lander College of Arts and Sciences
Due to numerous drawbacks with current modes of treatment for various ocular diseases, researchers are synthesizing drug dispensing contact lenses. The lenses will contribute to greater bioavailability of the drug, the minimization of negative side effects, and increased patient compliance. As treatment for glaucoma, in vivo studies have been conducted with latanoprost, timolol maleate, and brimonidine tartrate-eluting lenses, and have succeeded in reducing intraocular pressure to desired values (Ciolino et al., 2016), (Schultz and Mint, 2002). As treatment for fungal keratitis, in vitro studies prove that econazole and natamycin-eluting contact lenses have been successful in killing 100% of fungi for ...
Is Stem Cell Transplantation A Practical Treatment For Macular Degeneration?, 2017 Assumption College
Is Stem Cell Transplantation A Practical Treatment For Macular Degeneration?, Zachary Slepchuk
No abstract provided.
A Sema3c Mutant Resistant To Cleavage By Furin (Fr-Sema3c) Inhibits Choroidal Neovascularization, 2016 Cancer Research and Vascular Biology Center
A Sema3c Mutant Resistant To Cleavage By Furin (Fr-Sema3c) Inhibits Choroidal Neovascularization, Shira Toledano, Huayi Lu, Agustina Palacio, Keren Ziv, Ofra Kessler, Shlomit Schaal, Gera Neufeld, Yoreh Barak
Open Access Articles
In age-related macular degeneration (AMD), abnormal sub retinal choroidal neovascularization (CNV) is a major cause of blindness. FR-sema3C is a point mutated form of semaphorin-3C that is resistant to cleavage by furin like pro-protein convertases (FPPC). We have found in previous work that FR-sema3C functions as an anti-angiogenic factor. In this study we investigated the possible use of FR-sema3C as an inhibitor of CNV. FR-sema3C inhibits VEGF as well as PDGF-BB signal transduction in endothelial cells and to less extent bFGF induced signal transduction using a mechanism that does not depend upon the binding of VEGF like the drugs that ...
Cost-Effectiveness Of Selective Laser Trabeculoplasty (Slt) Versus Argon Laser Trabeculoplasty (Alt) In Uncontrolled Open Angle Glaucoma Patients Having At Least One Full Previous Slt: An Economic Evaluation Alongside An Ongoing Randomized Controlled Clinical Trial, 2016 The University of Western Ontario
Cost-Effectiveness Of Selective Laser Trabeculoplasty (Slt) Versus Argon Laser Trabeculoplasty (Alt) In Uncontrolled Open Angle Glaucoma Patients Having At Least One Full Previous Slt: An Economic Evaluation Alongside An Ongoing Randomized Controlled Clinical Trial, Muhammad Rakibuz-Zaman
Electronic Thesis and Dissertation Repository
Background and objective: ALT and SLT are both safe and effective for glaucoma treatment. We performed a cost-effectiveness analysis (CEA) of SLT versus ALT for a six-month follow-up period in uncontrolled open angle glaucoma patients having at least one full previous SLT from an ongoing RCT. Methods: Trial based treatment costing and IOP reduction at 6-month follow-up from baseline for both intervention arms were calculated. A decision tree model was developed considering possible clinical pathways of patients undergoing repeat laser trabeculoplasty. CEA among ALT and SLT was done, and ICERs were calculated from both societal and ministry perspective. One way ...
Impact Of Microbiota On Resistance To Ocular Pseudomonas Aeruginosa-Induced Keratitis, 2016 Harvard Medical School
Impact Of Microbiota On Resistance To Ocular Pseudomonas Aeruginosa-Induced Keratitis, Abirami Kugadas, Stig Hill Christiansen, Saiprasad Sankaranarayanan, Neeraj K. Surana, Stefanie Gauguet, Ryan Kunz, Raina Fichorova, Thomas Vorup-Jensen, Mihaela Gadjeva
Pediatric Publications and Presentations
The existence of the ocular microbiota has been reported but functional analyses to evaluate its significance in regulating ocular immunity are currently lacking. We compared the relative contribution of eye and gut commensals in regulating the ocular susceptibility to Pseudomonas aeruginosa-induced keratitis. We find that in health, the presence of microbiota strengthened the ocular innate immune barrier by significantly increasing the concentrations of immune effectors in the tear film, including secretory IgA and complement proteins. Consistent with this view, Swiss Webster (SW) mice that are typically resistant to P. aeruginosa-induced keratitis become susceptible due to the lack of microbiota. This ...
Advances In Gene Therapy For Diseases Of The Eye, 2016 University of Massachusetts Medical School
Advances In Gene Therapy For Diseases Of The Eye, Lolita Petit, Hemant Khanna, Claudio Punzo
UMass Metabolic Network Publications
Over the last few years, huge progress has been made with regard to the understanding of molecular mechanisms underlying the pathogenesis of neurodegenerative diseases of the eye. Such knowledge has led to the development of gene therapy approaches to treat these devastating disorders. Challenges regarding the efficacy and efficiency of therapeutic gene delivery have driven the development of novel therapeutic approaches, which continue to evolve the field of ocular gene therapy. In this review article, we will discuss the evolution of preclinical and clinical strategies that have improved gene therapy in the eye, showing that treatment of vision loss has ...
Results At 2 Years After Gene Therapy For Rpe65-Deficient Leber Congenital Amaurosis And Severe Early-Childhood-Onset Retinal Dystrophy, 2016 Oregon Health & Science University
Results At 2 Years After Gene Therapy For Rpe65-Deficient Leber Congenital Amaurosis And Severe Early-Childhood-Onset Retinal Dystrophy, Richard G. Weleber, Mark E. Pennesi, David J. Wilson, Shalesh Kaushal, Laura R. Erker, Lauren Jensen, Maureen T. Mcbride, Terence R. Flotte, Margaret Humphries, Roberto Calcedo, William W. Hauswirth, Jeffrey D. Chulay, J. Timothy Stout
Open Access Articles
PURPOSE: To provide an initial assessment of the safety of a recombinant adeno-associated virus vector expressing RPE65 (rAAV2-CB-hRPE65) in adults and children with retinal degeneration caused by RPE65 mutations.
DESIGN: Nonrandomized, multicenter clinical trial.
PARTICIPANTS: Eight adults and 4 children, 6 to 39 years of age, with Leber congenital amaurosis (LCA) or severe early-childhood-onset retinal degeneration (SECORD).
METHODS: Patients received a subretinal injection of rAAV2-CB-hRPE65 in the poorer-seeing eye, at either of 2 dose levels, and were followed up for 2 years after treatment.
MAIN OUTCOME MEASURES: The primary safety measures were ocular and nonocular adverse events. Exploratory efficacy measures ...
Prenylated Retinal Ciliopathy Protein Rpgr Regulates Ciliary Localization Of Joubert Syndrome-Associated Protein Inpp5e In Cooperation With Pde6, 2016 University of Massachusetts Medical School
Prenylated Retinal Ciliopathy Protein Rpgr Regulates Ciliary Localization Of Joubert Syndrome-Associated Protein Inpp5e In Cooperation With Pde6, Wei Zhang, Kollu N. Rao, Linjing Li, Manisha Anand, Hemant Khanna
UMass Center for Clinical and Translational Science Research Retreat
Ciliary dysfunction is an underlying cause of severe human disorders (collectively called ciliopathies), such as retinitis pigmentosa (RP), Joubert Syndrome (JBTS), and Bardet-Biedl Syndrome. Ciliary proteins form distinct functional networks for localization to cilia as well as regulation of ciliary function. However, not much is known about the mechanism of ciliary localization and function of RPGR (retinitis pigmentosa GTPase regulator), a ciliary protein frequently associated with RP worldwide. Using tandem mass spectrometry analysis, we show that RPGR interacts with two JBTS-associated proteins: PDE6Π (delta subunit of Phosphodiesterase; a prenyl-binding protein) and INPP5E (inositol polyphosphate-5-phosphatase 5E; a ciliary cargo). Whereas PDE6Π ...
The Carboxyl Terminal Mutational Hotspot Of The Ciliary Disease Protein Rpgrorf15 (Retinitis Pigmentosa Gtpase Regulator) Is Glutamylated In Vivo, 2016 University of Massachusetts Medical School
The Carboxyl Terminal Mutational Hotspot Of The Ciliary Disease Protein Rpgrorf15 (Retinitis Pigmentosa Gtpase Regulator) Is Glutamylated In Vivo, Kollu N. Rao, Manisha Anand, Hemant Khanna
Open Access Articles
Mutations inRPGR(ORF15)(retinitis pigmentosa GTPase regulator) are a major cause of inherited retinal degenerative diseases. RPGR(ORF15)(1152 residues) is a ciliary protein involved in regulating the composition and function of photoreceptor cilia. The mutational hotspot in RPGR(ORF15)is an unusual C-terminal domain encoded by exon ORF15, which is rich in polyglutamates and glycine residues (Glu-Gly domain) followed by a short stretch of basic amino acid residues (RPGR(C2)domain; residues 1072-1152). However, the properties of the ORF15-encoded domain and its involvement in the pathogenesis of the disease are unclear. Here we show that RPGR(ORF15)is glutamylated ...
Activation Of Mtorc1 Improves Cone Cell Metabolism And Extends Vision In Retinitis Pigmentosa Mice: A Dissertation, 2016 University of Massachusetts Medical School
Activation Of Mtorc1 Improves Cone Cell Metabolism And Extends Vision In Retinitis Pigmentosa Mice: A Dissertation, Aditya Venkatesh
GSBS Dissertations and Theses
Retinitis Pigmentosa (RP) is an inherited photoreceptor degenerative disease that leads to blindness and affects about 1 in 4000 people worldwide. The disease is predominantly caused by mutations in genes expressed exclusively in the night active rod photoreceptors; however, blindness results from the secondary loss of the day active cone photoreceptors, the mechanism of which remains elusive. Here, we show that the mammalian target of rapamycin complex 1 (mTORC1) is required to delay the progression of cone death during disease and that constitutive activation of mTORC1 is sufficient to maintain cone function and promote cone survival in RP. Activation of ...