Open Access. Powered by Scholars. Published by Universities.®

Laboratory and Basic Science Research Commons

Open Access. Powered by Scholars. Published by Universities.®

1,084 Full-Text Articles 1,990 Authors 460,527 Downloads 156 Institutions

All Articles in Laboratory and Basic Science Research

Faceted Search

1,084 full-text articles. Page 5 of 44.

Comparing Cognition In Women Between The Ages Of (18-40) And (40-70), Annalyn Smith 2021 University of Arkansas, Fayetteville

Comparing Cognition In Women Between The Ages Of (18-40) And (40-70), Annalyn Smith

Biological Sciences Undergraduate Honors Theses

Currently, 5.8 million Americans are living with Alzheimer’s disease (AD), which is a degenerative brain disease. It is currently the sixth-leading cause of death in the United States (Alzheimer’s Association, 2019). AD also possesses a financial burned on the United States, estimated to cost $290 billion in 2019. Sufferers of AD insidiously lose their memory, personality, and judgment abilities until there are no longer recognizable by their loved ones. Currently, the best chance suffers of AD have is to catch the disease early on to slow its effects. This is due to the fact that treatment can only prevent and …


Assessing The Outcomes Of Blocking Ccl2-Ccr2 Signaling Axis On Breast Cancer Brain Metastasis, Yutao Qi 2021 The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences

Assessing The Outcomes Of Blocking Ccl2-Ccr2 Signaling Axis On Breast Cancer Brain Metastasis, Yutao Qi

The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences Dissertations and Theses (Open Access)

Breast cancer brain metastases have remained one of the most intense challenges for precision cancer therapeutics, but current treatment options are limited and not curative. Recently, our lab reported that adoptive PTEN downregulation in metastatic breast tumor cells activates PI3K/NF-ƙB signaling and increases the secretion of the chemokine CCL2, which enhances the chemotaxis of CCR2+ myeloid cells, a major subpopulation of bone marrow-derived myeloid cells (BMDMs), from peripheral blood into the brain tumor microenvironment (TME), eventually promoting brain metastasis outgrowth by driving immune suppression. Here, in this project we have been aiming to develop effective therapies by immune-modulating the …


Implementing Crispr-Cas9 Gene Editing System In Microbotryum., Stevana Reese Schauer 2021 University of Louisville

Implementing Crispr-Cas9 Gene Editing System In Microbotryum., Stevana Reese Schauer

College of Arts & Sciences Senior Honors Theses

Microbotryum dianthorum is a species of smut fungus which causes infection in plants in the Dianthus genus. The infection process is aided by small-secreted proteins known as effectors. Effectors may aid in the infection process because they are secreted by the fungus and can enter plant tissue, possibly to facilitate infection and/or manipulate the host. Thus, it is of interest to investigate the genes for these effectors to determine their role in the infection process. One method to study genes is through gene disruption, via the CRISPR/Cas9 gene editing system, but this has not yet been implemented in Microbotryum dianthorum …


The Impact Of Natural Rubber As A Toughening Agent On The Strength, Degradability, And Toxicity Of An Algae-Based Bioplastic, Megan Driscoll 2021 University of Maine - Main

The Impact Of Natural Rubber As A Toughening Agent On The Strength, Degradability, And Toxicity Of An Algae-Based Bioplastic, Megan Driscoll

Honors College

With the growing attention on widespread plastic usage and its impact on the environment and human health, the need for sustainable alternatives to petroleum-based plastics is more important than ever. One of the most promising solutions is bioplastics; however, current bioplastics struggle to compete with the material properties of petroleum- based plastics. Agar is a sustainable algae-derived hydrocolloid polysaccharide that can be used for bioplastics and biofilms. Despite promising characteristics, bioplastics made from agar are brittle. Common additives, such as the plasticizer glycerol, offset brittleness but sacrifice strength in return. This study looks at the impact of natural rubber as …


Intermachine Reliability Of The Velotron During Wingate Anaerobic Testing, Caleb Christie 2021 Utah State University

Intermachine Reliability Of The Velotron During Wingate Anaerobic Testing, Caleb Christie

All Graduate Plan B and other Reports

Purpose: The purpose of this study was to evaluate inter-machine reliability of the Velotron electromagnetically-braked cycle ergometer for the Wingate anaerobic test (WAnT) with power-trained athletes. Methods: Anaerobic athletes participating in football or track and field at a National Collegiate Athletics Association Division I program were recruited for participation in the study. Each participant visited the laboratory 3 times (1 familiarization trial, 2 experimental trials) within 2 weeks and at least 24 hours between visits. Participants completed a “practice trial” on the first visit to familiarize themselves with the 30-second WAnT. The WAnT procedure included a 5-minute warmup at a …


Validation Of A Novel Trpm8 Knockout Mouse Model, Bilan Mohamed 2021 University of Southern Maine

Validation Of A Novel Trpm8 Knockout Mouse Model, Bilan Mohamed

Thinking Matters Symposium

Recent studies suggest that the use of thermoregulatory treatments that affect brown fat may help curb obesity. However it is unknown how these treatments may affect bone homeostasis. Our work has focused on the transient receptor potential melastatin (TRPM8) protein, which is responsible for detecting colder temperatures in sensory neurons. Previous work within the Motyl laboratory has found that Trpm8 plays a role in bone acquisition. Mice with a global deletion of the Trpm8 gene have reduced trabecular bone volume fraction due to reduced bone formation by osteoblasts. However, it is unclear whether sensory neuron or osteoblast-mediated expression of Trpm8 …


Effect Of Temperature On Questing Activity Of Ixodes Scapularis In A Laboratory Setting, Molly Meagher 2021 University of Southern Maine

Effect Of Temperature On Questing Activity Of Ixodes Scapularis In A Laboratory Setting, Molly Meagher

Thinking Matters Symposium

Understanding deer tick (Ixodes scapularis) behavior is increasingly important due to the species’ northerly range expansion, and consequently, increased human contact. Deer ticks are a major public health concern, as they are the primary vector of Lyme disease and other serious diseases. Differences in questing behavior are of considerable interest as fluctuating weather due to climate change effect the species’ range expansion and time spent questing. Although previous research has examined temperature preferences of tick behavior, there are conflicting reports about direct effects on questing activity. Therefore, I addressed the relationship between temperature and questing behavior of adult deer ticks. …


Perivascular Adipose Tissue In Relation To Diet, Thermogenesis And Cardiovascular Health, Ginger Paquette, Caitlin Stieber, Ashely Soucy, Benjamin Tero, Lucy Liaw 2021 University of Southern Maine

Perivascular Adipose Tissue In Relation To Diet, Thermogenesis And Cardiovascular Health, Ginger Paquette, Caitlin Stieber, Ashely Soucy, Benjamin Tero, Lucy Liaw

Thinking Matters Symposium

Adipose tissue is a diverse and crucial component to vascular health due to its role in energy storage and heat production. The primary function of white adipose tissue (WAT) is energy storage while the function of mitochondria-rich brown adipose tissue (BAT) is heat production. Perivascular adipose tissue (PVAT), which surrounds blood vessels, contains both WAT and BAT adipocytes. Dietary calorie restriction is associated with increased lifespan with decreased adiposity. Increased prevalence of WAT-like PVAT, due to high-fat diets and obesity, leads to increased metabolic disfunction and cardiovascular-disease. We used a calorie-restriction model in C57BL6/J mice to test the hypothesis that …


Importance Of Understanding Genetic Predisposition, Andrew Carter 2021 Southern Maine Community College

Importance Of Understanding Genetic Predisposition, Andrew Carter

Thinking Matters Symposium

Hypersensitivity reactions can be sudden and are often fatal. Many of these reactions occur as a result of allergies to prescribed medication. Adverse drug reactions or (ADR’s) were show in a recent study to affect over 6% of hospitalized patients and resulted in over 100,000 deaths a year in the US alone. This poster will review the importance of understanding genetic predisposition, through articles about possible genetic causes of allergies to penicillin and other beta-lactams. Changes in a specific HLA gene located on chromosome 6 showed a correlation to penicillin reactions in over 600,000 participants. Another study found a conclusive …


Purple Variety Of Sweet Potato, Ming Gao 2021 Prairie View A&M University

Purple Variety Of Sweet Potato, Ming Gao

Datasets Collection

Purple sweet potatoes are versatile enough to be used in cupcakes as well as ice cream. However, when cooked in their natural state, they are dry and starchy, prompting most to use the purple sweet potatoes as flour for baked goods, or in some cases, for ice cream. Dr. Gao wants his variety of purple sweet potato to be more palatable. Gao earned the coveted USDA-National Institute of Food and Agriculture 1890 Institution Capacity Building Grant to support his research. The project will focus on developing a variety that is enjoyable in its natural state and is moist and tastefully …


S.O.S. For Covid-19 (Subjective And Objective Screening): Are Asymptomatic Cases Truly Without Warning Signs?, Nathan Hughes 2021 Wayne State Unviersity

S.O.S. For Covid-19 (Subjective And Objective Screening): Are Asymptomatic Cases Truly Without Warning Signs?, Nathan Hughes

Honors College Theses

This study investigates the usefulness of five objective signs, evaluates relationships between objective signs with symptoms of COVID-19, and assesses the accuracy of ambient forehead temperature with tympanic temperature. Data were collected at Wayne State University. Blood oxygen levels and blood pressure were measured via pulse oximeters and blood pressure cuff. Body temperature was measured with a thermometer via the temple and the forehead. Smell was tested with two scented markers. None of the 29 participants confirmed a positive COVID-19 test. Therefore, only relationships between vital sign measurements were reported. No significant correlations were revealed upon analysis. Infrared and temporal …


The Effect Of A Single Dose Of Beetroot Juice On Speed, Strength, And Power In Healthy Recreationally Active Females., Maya A. Hammer, Ryan Kenney, Katie Price, Chandler Riley, Alyssa Riley, Courtney Merrill, Adam Pennell, Rachel Tan 2021 Pepperdine University

The Effect Of A Single Dose Of Beetroot Juice On Speed, Strength, And Power In Healthy Recreationally Active Females., Maya A. Hammer, Ryan Kenney, Katie Price, Chandler Riley, Alyssa Riley, Courtney Merrill, Adam Pennell, Rachel Tan

Seaver College Research And Scholarly Achievement Symposium

Nitrate-rich beetroot juice (BR) improves muscle contraction which is relevant for intense intermittent-type sports. However, few studies have examined the effect of BR ingestion on performance using sport-specific exercise protocols. Moreover, there is a scarcity of research that include female participants which limits real world application given that females potentially have different responses to BR ingestion. The purpose of the present study was to examine the effect of BR supplementation on speed, acceleration, strength and power before and after fatigue in females. In a double-blind, randomized crossover design, 15 recreationally active females consumed BR and nitrate-depleted placebo juice (PL) ~2.5 …


The Effects Of Nitrate-Rich Beetroot Juice Supplementation On Nonverbal Executive Function In Healthy Recreationally Active Females, Chandler F. Riley, Alyssa Riley, Maya A. Hammer, Ryan Kenney, Katie Price, Courtney Merrill, Adam Pennell, Rachel Tan 2021 Pepperdine University

The Effects Of Nitrate-Rich Beetroot Juice Supplementation On Nonverbal Executive Function In Healthy Recreationally Active Females, Chandler F. Riley, Alyssa Riley, Maya A. Hammer, Ryan Kenney, Katie Price, Courtney Merrill, Adam Pennell, Rachel Tan

Seaver College Research And Scholarly Achievement Symposium

Nitrate-rich beetroot juice (BR) supplementation has been reported to preserve executive function (i.e. decision making and reaction time) before and after a simulated soccer match in recreationally active males, which may be due to enhanced cerebral blood flow. However, the literature examining the physiological response following BR ingestion in females is scarce, which hampers the extrapolation of results since physiological sex-differences may exist. Therefore, the purpose of this study was to assess if BR ingestion influenced executive function in an unfatigued and fatigued state in healthy recreationally active females. In a double-blind, randomized crossover design, 20 females consumed 140 mL …


Differential Effects Of Kim-1 In Subcutaneous And Orthotopic Renca Models Of Kidney Cancer, Demitra M. Yotis DY 2021 The University of Western Ontario

Differential Effects Of Kim-1 In Subcutaneous And Orthotopic Renca Models Of Kidney Cancer, Demitra M. Yotis Dy

Electronic Thesis and Dissertation Repository

Renal Cell Carcinoma (RCC) is the most common and fatal type of kidney cancer. Over 30% of patients that are diagnosed with RCC exhibit metastases. Almost 88% of patients with distant metastases succumb to the disease within 5 years of diagnosis. Kidney Injury Molecule-1 (KIM-1) is a cell surface glycoprotein that is not expressed in a healthy kidney but becomes highly expressed on proximal tubular epithelial cells (PTECs) following injury. Data from the Cancer Genome Atlas (TCGA) reveals that >90% of RCC tumours express KIM-1 mRNA and that higher expression levels correlate with increased overall survival rates of patients. The …


The Effect Of Changing Substrate On Arctic Aquatic Invertebrates Abundance, Tom Dolman 2021 University of Maine at Farmington

The Effect Of Changing Substrate On Arctic Aquatic Invertebrates Abundance, Tom Dolman

Michael D. Wilson Symposium

Climate change is directly affecting tundra ecosystems in northern regions, and warming temperatures have caused discontinuous permafrost and thawing sediments across the region. This project investigates how increasing erosion and the foraging patterns of migratory snow geese may degrade habitat for aquatic invertebrates in the upper Mast River, located in Wapusk National Park, Manitoba, Canada. In the past two decades, many of the important species of aquatic invertebrates have shown declines. Declining invertebrate populations are predicted to affect aquatic ecosystems and decrease the resources available to shorebirds and waterfowl, which breed and migrate through this area.


Corticospinal Excitability During A Perspective Taking Task As Measured By Tms-Induced Motor Evoked Potentials, Elizabeth Murray, Janet Brenya, Katherine Chavarria, Karen J. Kelly, Anjel Fierst, Nathira Ahmad, Caroline Anton, Layla Shaffer, Kairavi Kapila, Logan Driever, Kayla Weaver, Caroline Dial, Maya Crawford, Iso Hartman, Tommy Infantino, Fiona Butler, Abigail Straus, Shakeera L. Walker, Brianna Balugas, Matthew Pardillo, Briana Goncalves, Julian Keenan 2021 Hackensack Meridian Health

Corticospinal Excitability During A Perspective Taking Task As Measured By Tms-Induced Motor Evoked Potentials, Elizabeth Murray, Janet Brenya, Katherine Chavarria, Karen J. Kelly, Anjel Fierst, Nathira Ahmad, Caroline Anton, Layla Shaffer, Kairavi Kapila, Logan Driever, Kayla Weaver, Caroline Dial, Maya Crawford, Iso Hartman, Tommy Infantino, Fiona Butler, Abigail Straus, Shakeera L. Walker, Brianna Balugas, Matthew Pardillo, Briana Goncalves, Julian Keenan

Department of Biology Faculty Scholarship and Creative Works

Only by understanding the ability to take a third-person perspective can we begin to elucidate the neural processes responsible for one’s inimitable conscious experience. The current study examined differences in hemispheric laterality during a first-person perspective (1PP) and third-person perspective (3PP) taking task, using transcranial magnetic stimulation (TMS). Participants were asked to take either the 1PP or 3PP when identifying the number of spheres in a virtual scene. During this task, single-pulse TMS was delivered to the motor cortex of both the left and right hemispheres of 10 healthy volunteers. Measures of TMS-induced motor-evoked potentials (MEPs) of the contralateral abductor …


Identification Of Parasites From Wisconsin And Minnesota Diving Ducks In The Winona Area, Morgan Condon, Kimberly Bates 2021 Winona State University

Identification Of Parasites From Wisconsin And Minnesota Diving Ducks In The Winona Area, Morgan Condon, Kimberly Bates

Ramaley Celebration

Many different diving ducks in the Midwest are hosts to parasites like cestodes, trematodes, and nematodes. These parasites often live in the ducks gastrointestinal tract and use the ducks chyme and intestinal tissues to thrive. For this research, local hunters volunteered to donate diving duck carcasses they harvested. Ducks donated were cut open on the ventral side from esophagus to cloaca. Major organs were separated into bottles containing 0.85% saline, shaken, settled, and then the supernatant removed multiple times to isolate any parasites residing within the duck’s organs and tissues. Any parasites found in dissected diving ducks organ wash were …


Toxic Effect Of Crotalus Adamanteus Acidic Phospholipase A2 On Mcf-7 Cell Line, Daniel J. Petra 2021 University of South Dakota

Toxic Effect Of Crotalus Adamanteus Acidic Phospholipase A2 On Mcf-7 Cell Line, Daniel J. Petra

Honors Thesis

We are investigating the effect of Crotalus adamanteus acidic phospholipase A2 on MCF-7 cells using the MTS assay. Understanding these interactions and isolated effects is critical to developing new ways to treat envenomation. By understanding the effects of individual toxins within a whole venom, we are set to better understand the effects of the whole venom and investigate synergistic actions between venom toxins. In this paper, we are quantifying the amount of MCF-7 cell death caused by Crotalus adamanteus phospholipase A2 on MCF-7 using the MTS assay. Analysis of the amount of cells death caused by the phospholipase …


Laboratory Techniques In Public Health: Bi-520 Introduction To Public Health Lab Manual, Anuradha Srivastava 2021 CUNY Queensborough Community College

Laboratory Techniques In Public Health: Bi-520 Introduction To Public Health Lab Manual, Anuradha Srivastava

Open Educational Resources

This laboratory manual for a foundations course Public Health is unique in the sense that very rarely, a foundations course in the field has a lab component offered with the lecture. This lab manual provides basic laboratory exercises that could be useful to introduce undergraduate students to the basics of public health laboratory practices.


Morph- And Sex-Specific Differences In Corticosterone Of The Arizona Tiger Salamander (Ambystoma Mavortium Nebulosum), Megan Zerger 2021 Murray State University

Morph- And Sex-Specific Differences In Corticosterone Of The Arizona Tiger Salamander (Ambystoma Mavortium Nebulosum), Megan Zerger

Scholars Week

Life history morph, sex, and body condition are traits that may influence stress within salamander populations because of differences in physiology and environmental conditions. Given widespread declines and the effects chronic stress can have on amphibian health, it is important to understand within-population drivers of stress and how population level variation may influence population viability. Thus, the objective of our study was to assess how corticosterone varies within the Arizona tiger salamander (Ambystoma mavortium nebulosum) population at the Mexican Cut Nature Preserve. We used a non-invasive skin swabbing method to collect baseline and elevated corticosterone from paedomorph (aquatic …


Digital Commons powered by bepress