Laboratory and Basic Science Research Commons^™

Optimizing Itpa R178c Assays Via Hplc, Jared J. Reisnouer, Wally D. Pines

2026 Symposium

Two of the major nucleotide bases in DNA and RNA (Adenine and Guanine) are derived from the purine Inosine Monophosphate (IMP). IMP may occasionally form the noncanonical nucleotide Inosine Triphosphate (ITP) within the cell and become incorporated into DNA during replication, leading to potentially lethal errors. To combat this, human cells produce the “housekeeping” enzyme Inosine Triphosphatase (ITPA) to revert ITP to IMP. A mutation of this protein that replaces the 178th amino acid Arginine with Cysteine (R178C) is associated with a fatal infantile encephalopathy. Previous assessments of enzyme-substrate binding and catalysis for ITPA variants have been run at a …

Short Lab Report- Presentation, Wroud Saleh, Yevgeniy Grigoryev

Open Educational Resources

No abstract provided.

Glioblastoma Multiforme: A Pathologic Breakdown Of One Of Neuro-Oncology's Greatest Challenges, Rosemary Westphal, Ms

Student Papers, Posters & Projects

Glioblastoma multiforme is the most common adult primary tumor with a grim median survival time of 14.6 months. It is characterized by rapid progression, diffuse infiltration, and rather poor clinical outcomes. The WHO classifies GBM as a grade 4 diffuse astrocytic tumor with distinctive histopathological features including high mitotic activity, marked cellular atypia, microvascular proliferation, and necrosis. Advances in molecular diagnostics have further classified GMB into IDH-wild type and IDH-mutant subtypes, with important therapeutic and prognostic implications. Key genetic alterations, such as EGFR amplification, PTEN loss, TP53 mutations, TERT promoter mutations, and MGMT methylations, have been identified as contributing to …

Emerald Bowfin Amia Ocellicauda Ecology In Three Minnesota Lakes: Abundance, Age Analysis, Diet Composition, And Stable Isotope Food Web, Griffin Blegen

Biology Graduate Theses

The Emerald Bowfin Amia ocellicauda is a predatory fish species native to Minnesota that has often been discriminated against. This research expands ecological knowledge of Bowfin in Minnesota and provides population data to guide future management. Bowfin were sampled in the spring of 2024 and 2025 and a multi-census mark–recapture model was used to estimate abundance in three lakes. Estimated populations were 196 individuals in Little Toad Lake (95% CI: 116, 242) or 4.4 fish/littoral ha (95% CI: 2.7,5.7), 657 in Lake Sarah (95% CI: 436, 1014) or 4.2 fish/littoral ha (95% CI: 3.0, 6.7), and 612 in Gun Lake …

Perspective In Biomedical Sciences Field: Education And Career, Fitra Adi Prayogo, Mellyaning Oktaviani Sonya Kirana Sari, Isy Royhanaty, Poppy Fansisca Amelia, Heni Wijayanti, Ni Nyoman Maryaningtyas Adinatha, Dyah Wulandari, Anto Budiharjo

Chulalongkorn Medical Journal

Abstract

Background: The biomedical sciences represent a dynamic and multidisciplinary field pivotal to advancing human health through research, education, and innovation.

Objective(s): This review explores the evolving landscape of biomedical education and career pathways, driven by technological advancements, interdisciplinary integration, and global health demands.

Methods: We examine the distinctions between biomedical science, biomedicine, and biomedical engineering, highlighting their historical origins and contemporary roles. The education and career trajectories of biomedical laboratory scientists (BLS) are analysed, emphasising diverse competencies, core curricula, and the impact of regional policies on professional qualifications. Emerging trends and ethical/regulatory challenges are discussed.

Result(s): Key trends include …

Effects Of Phosphorus Addition On Fungal Colonization In Little Bluestem, Isabel M. Zanartu, Alexx Nicholas, Mckenzie Goodwyn, Connor Morozumi

The Cardinal Edge

Little Bluestem (Schizachyrium scoparium) is a North American perennial grass that is able to tolerate high heat and droughts. This is possible by its extensive underground root network that taps deep into the ground for resources. Within the same soil systems are fungal endophytes, which are symbionts that live within plant tissues including roots. Fungal endophytes can affect host growth and nutrient acquisition, but the functional role these endophytes play in plant health may be dependent on the abiotic conditions the plant is facing. In the modern era, one large driver of abiotic conditions is synthetic fertilizer use which alters …

Targeting Il-1 Signaling In Recessive Dystrophic Epidermolysis Bullosa: Improved Survival And Pathogenesis In Rdeb Mouse Model, Rahim Hirani

NYMC Student Theses and Dissertations

Recessive dystrophic epidermolysis bullosa (RDEB) is a devastating inherited blistering disorder caused by biallelic mutations in COL7A1, resulting in deficiency of type VII collagen (C7) and loss of dermal–epidermal adhesion. Beyond mechanical fragility, RDEB is characterized by chronic inflammation, progressive fibrosis, impaired wound healing, severe pain, and an exceptionally high risk of aggressive cutaneous squamous cell carcinoma. Emerging transcriptomic and cytokine profiling studies have identified interleukin-1 alpha (IL-1α) as a dominant and temporally upstream inflammatory mediator in RDEB skin. The central hypothesis of this dissertation is that IL-1α signaling functions as a key initiator and amplifier of inflammation-driven pathology …

Physical And Chemical Study Of Interaction In The Urea–Valine System For The Creation Of Modified Liquid Nitrogen Fertilizers, Suvonkul Erkhanovich Nurmonov, Saydullo Khamidovich Azimov

Chemical Technology, Control and Management

This study reports the results of a comprehensive physicochemical investigation of the urea–L-valine binary system, conducted to validate its potential as a modified base for liquid nitrogen fertilizers.

By utilizing the methods of isomolar series, visual-polythermal analysis, X-ray phase (XRD) and single-crystal X-ray diffraction (SC-XRD), synchronous thermal analysis (TGA/DTA), and FT-IR spectroscopy, it was determined that the interaction between components in both aqueous solutions and the solid phase results in the formation of molecular associates stabilized by hydrogen bonds, without the formation of new stoichiometric compounds.

The concentration and temperature limits of the system's stability were defined, revealing a reduction …

Trophic Structure And Mercury Bioaccumulation In Walleye And Yellow Perch In The Upper And Lower Red Lake Basins, Marissa Pribyl

Biology Graduate Theses

Mercury is a persistent global contaminant that biomagnifies through aquatic food webs, with dietary and environmental factors serving as the primary drivers of accumulation in fishes. Trophic structure and methylmercury dynamics in Walleye (ogaa; Sander vitreus) and Yellow Perch (asaawens; Perca flavescens) were investigated in the Upper and Lower Red Lake basins in Red Lake, Minnesota, during 2024-2025. Diets of Walleye and Yellow Perch were assessed through stomach dissections, and tissue samples from both species were analyzed for total mercury concentrations. Additional analyses included shiners (gigoozens; Notropis spp., Hudsonius spp.) along with a variety of freshwater fish and …

Design And Functionalization Of A Hierarchical Cu-Btc/Zeolite A4 Composite With Tannic Acid For High-Performance Heavy Metal Removal: Synergistic Adsorption Mechanisms, Kinetic Insights, And Reusability Evaluation, Samar A. Salim

Nanotechnology Research Centre

This study presents the development of a novel multifunctional adsorbent Cu-BTC (HKUST-1)/Zeolite A4 composite functionalized with tannic acid—for the efficient removal of toxic heavy metal ions (Pb²⁺, Cd²⁺, Cr(VI)) from aqueous solutions. The composite was synthesized via a solvothermal process and systematically characterized by XRD, FTIR, SEM-EDS, and BET surface area analysis. The resulting material exhibited a high specific surface area (1050 m²/g), a hierarchical pore structure, and a homogeneous distribution of active sites. Batch adsorption experiments revealed superior adsorption capacities of 220 mg/g for Pb²⁺, 180 mg/g for Cd²⁺, and 150 mg/g for Cr(VI) at pH 6, surpassing those …

Classification Of Venomous Snakes In Thailand Based On The Melting Temperature Specificity Of Target Genes Using Sybr Green Real-Time Pcr, Sunutcha Suntrarachun, Tanapong Tawan, Lawan Chanhome

The Thai Journal of Veterinary Medicine

The SYBR Green real-time PCR was developed for the rapid and accurate classification of major venomous snakes in Thailand. The method relies on melting temperature (Tm) discrimination of target genes from mitochondrial ribosomes. Short single-stranded primers were designed to specifically amplify synthetic DNA of venomous snakes in a single reaction (singleplex), producing distinct melting peaks. Neurotoxic snakes such as cobras (Naja spp.), king cobra (Ophiophagus hannah) and kraits (Bungarus spp.) exhibited melting peaks ranging from 81.00 to 86.00°C, whereas hematotoxic snakes, including the Siamese Russell's viper (Daboia siamensis), Malayan pit viper (Calloselasma rhodostoma …

The Prophylactic Effect Of Aqueous Chamomile Extract On Nitrogen Dioxide Exposed Mice, Mariam Kbaysi, Ruzanna Petrosyan, Jamilah Borjac

BAU Journal - Science and Technology

Nitrogen dioxide (NO₂) a gaseous air pollutant resulting from industrial activities. It has been associated to various diseases related to oxidative stress and inflammation, including cardiovascular and nervous system disorders. Chamomile is mainly recognized for its high content of antioxidant flavonoids and is extensively utilized in herbal medicine for treating several disorders. The aim of this study is to examen the protective effects of chamomile extract against NO₂-induced damage in female mice. Balb/c mice were divided into four groups: control, chamomile extract, NO₂ derivatives, and NO₂ derivatives combined with chamomile extract. Upon completion of the study, Blood was …

Largemouth Bass Diet Changes Through The Summer In Central Minnesota, Ethan Kunz

Journal of Earth and Life Science

Largemouth Bass Micropterus nigricans is one of the most widespread fish species in the world, due in part to human introduction for angling. Largemouth Bass are opportunistic predators that will consume the largest prey they can often find; however, it has been shown that their diet mainly consists of invertebrates such as crayfish, with less focus on other fish species. This research aimed to determine what the Largemouth Bass diets in Central Minnesota consist of, and how they may change from the beginning of summer through the beginning of fall. A total of 174 Largemouth Bass were sampled from late …

Relationship Between Ratio Of Hybrid Sunfish To Their Parent Species And Latitude, Maxwell Kattner

Journal of Earth and Life Science

Hybrid sunfish of the Lepomis genus make up a generally small but notable portion of the population of sunfish in Minnesota lakes. Hybrid sunfish play a significant role in freshwater ecosystems across the United States. They are both morphologically and behaviorally distinct in some ways and similar in other ways to their parent species, causing them to both fill and compete with their parent species for niches occupied by multiple Lepomis species. Despite this, the ratio of hybrid sunfish to their parent species has never been properly explored. This study observed if the ratio of hybrid sunfish to their parent …

Cordgrass (Spartina Alterniflora) In Bio-Restoration Integrated Coastal Substrate (Brics): Restoring The New England Coastline., Kaitlyn Frances Shelley

UNH URC Open (2026 and after)

The Engineering for Agri-Environment Laboratory (EAE) is dedicated to developing nature-based solutions that enhance the resilience of agricultural and coastal ecosystems. The study involves current work (trial 2) underway in Spring 2026 and Summer 2026, integrating principles of sustainable engineering and plant science. BRICS, our Bio-Restoration Integrated Coastal Substrate, proposes a new way to reduce erosion, stabilize root structures, provide a critical habitat for wildlife, and promote vegetation throughout the coast.

Population Genomics Data For The Temperate Seagrass Posidonia Sinuosa, John Whale, Elizabeth Sinclair, Chanelle Webster, Nicole Said, Ankje Frouws, David Field, Kathryn Mcmahon

Research Datasets

Seagrasses are flowering plants commonly found along coastal waters, globally, where they provide important ecosystem services such as habitat provision, sequestering carbon, and sediment stabilization. They can reproduce both clonally and sexually where sexual reproduction can result in new, potentially adaptive, variants and the existence of both reproductive methods means that meadows can be highly variable in clonal diversity. The temperate seagrass, Posidonia sinuosa, occurs across a latitudinal gradient along the Western Australian coastline. Some meadows have experienced significant decline as a result of industrialisation and urbanisation of coastal areas. We investigated spatial genetic diversity, structure, and local adaptation using …

Methods Of Euthanasia In Laboratory Animals, Jaiden L. Corley

Williams Honors College, Honors Research Projects

The current honors research project will focus on the current use of carbon dioxide (CO2) as a means of euthanizing laboratory mice. CO2 is commonly used as a means of euthanizing laboratory animals and has been a standard of practice for mamy years. it is regarded as fast, painless, and efficient in overwhelming the respiratory control centers of the brainstem and resulting in respiratory depression and death. However, there continues to be debate and ongoing research regarding the 'humaneness' of this approach to euthanasia, and the American Veterinary Medicine Association (AVMA) suggests the inclusion of isoflurane (a volatile anesthetic) as …

Effectiveness Of Walleye Stocking In Winter Kill Lakes, Tyler Haus

Journal of Earth and Life Science

Winter kill events, marked by extensive fish mortality due to hypoxic conditions under the ice, are a common phenomenon in shallow eutrophic lakes, especially in the northern lakes in the northern United States. These events can drastically alter fish communities and often result in the elimination of top predators. Though these lakes will typically slowly bounce back to what they were in a couple of years. This study evaluates the effectiveness of walleye, Sander vitreus, stocking in lakes after a winter kill. For this study, four known winter kill lakes that are consistently stocked with walleye were studied. Pre- …

Assessing Urban–Rural Differences In Largemouth Bass Abundance In Minnesota, Jonathan Gates

Journal of Earth and Life Science

Urbanization can significantly alter freshwater ecosystems and influence sport fish populations. This study examined differences in largemouth bass Micropterus nigricans catch-per-unit-effort (CPUE) between urban and rural lakes in Minnesota and evaluated temporal changes between 2000–2010 and 2011–2021. Forty lakes (20 urban, 20 rural) were selected using Minnesota Department of Natural Resources fisheries survey data. Lakes were classified using NLCD 2019 land cover data, with ≥10% developed land designated as urban. Nonparametric analyses (Wilcoxon rank-sum and signed-rank tests) were used to assess spatial and temporal differences in CPUE. Results showed that rural lakes had consistently higher median CPUE than urban lakes …

Statewide Northern Pike Response To Minnesota’S 2018 Regulation Change, Elijah Wozniak

Journal of Earth and Life Science

Management of northern pike (Esox lucius) is challenging because it attempts to balance ecological sustainability with angler interests. Northern pike often stunt their own growth by diminishing the size and abundance of prey fish, their food source. Anglers are often displeased when small pike occur in high population densities. In response to populations of small northern pike, the DNR implemented a new, statewide northern pike regulation in 2018. The objective of this study was to determine whether the regulation successfully changed the mean weight, length, and CPUE of northern pike in Minnesota. The secondary objective was to test …