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Development Of A Tissue Specific Bioscaffold For Intestinal Stem Cell Culture, Sachin Kakar 2022 The University of Western Ontario

Development Of A Tissue Specific Bioscaffold For Intestinal Stem Cell Culture, Sachin Kakar

Electronic Thesis and Dissertation Repository

The generation of a tissue-specific intestinal hydrogel using small intestinal extracellular matrix (ECM) has the potential to support and promote the growth of intestinal organoids. In this study, we aimed to develop hydrogels derived exclusively from intestinal ECM or composites comprised of intestinal ECM combined with alginate, which may allow greater tuning of the hydrogel properties. A novel mouse intestinal decellularization protocol was developed and the ECM was characterized. Our analysis demonstrates that cellular and nuclear content was removed effectively, while preserving key ECM components. When decellularized ECM was used to generate hydrogels, the resulting ECM displayed bioactivity as demonstrated …


Distribution Of Serotypes And Antibiotic Resistance Of Invasive Pseudomonas Aeruginosa In A Multi-Country Collection, Shamima Nasrin, Nicolas Hegerle, Shaichi Sen, Joseph Nkeze, Sunil Sen, Jasnehta Permala-Booth, Myeongjin Choi, James Sinclair, Milagritos D. Tapia, Sadia Shakoor 2022 University of Maryland School of Medicine

Distribution Of Serotypes And Antibiotic Resistance Of Invasive Pseudomonas Aeruginosa In A Multi-Country Collection, Shamima Nasrin, Nicolas Hegerle, Shaichi Sen, Joseph Nkeze, Sunil Sen, Jasnehta Permala-Booth, Myeongjin Choi, James Sinclair, Milagritos D. Tapia, Sadia Shakoor

Department of Pathology and Laboratory Medicine

Background: Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of acute and chronic infections and is frequently associated with healthcare-associated infections. Because of its ability to rapidly acquire resistance to antibiotics, P. aeruginosa infections are difficult to treat. Alternative strategies, such as a vaccine, are needed to prevent infections. We collected a total of 413 P. aeruginosa isolates from the blood and cerebrospinal fluid of patients from 10 countries located on 4 continents during 2005-2017 and characterized these isolates to inform vaccine development efforts. We determined the diversity and distribution of O antigen and flagellin types and …


Regulation Of Skeletal Muscle Plasticity By The Gut Microbiome, Taylor R. Valentino 2022 University of Kentucky

Regulation Of Skeletal Muscle Plasticity By The Gut Microbiome, Taylor R. Valentino

Theses and Dissertations--Physiology

Recent evidence suggests that the gut microbiome could play a role in skeletal muscle plasticity, providing novel treatments for muscle wasting diseases and/or performance enhancements. I first sought to determine if the gut microbiome is necessary for skeletal muscle adaptation to exercise. Forty-two, four-month old, female C57Bl/6J underwent nine weeks of weighted wheel running or remained in cage with a locked wheel, without or without the administration of antibiotics (treated). In response to wheel running, I found that antibiotic depletion of the microbiome led to a blunted hypertrophic response in the soleus muscle as measured by normalized muscle wet weight …


Investigating The Effects Of In-Vivo Therapeutics Hypoxia Treatment Paradigms In Neurite Outgrowth Patterns, Jae Hyun Yoo 2022 University of Kentucky

Investigating The Effects Of In-Vivo Therapeutics Hypoxia Treatment Paradigms In Neurite Outgrowth Patterns, Jae Hyun Yoo

Theses and Dissertations--Medical Sciences

Spinal cord injury, specifically in the cervical C3-C4 region of the cervical spine, contributes to impaired breathing and a diminished quality of life. Therefore it is important to find effective and safe therapeutics to restore breathing function. Indeed, there are a myriad of research being performed in addition to valuable collaboration amongst different institutions. As such, inspired by a previous experiment, we decided to test our hypothesis that an enriching environment consisting of different hypoxic environments - sustained and intermittent hypoxia alongside normoxia - would result in neurite outgrowth. Moreover, we hypothesized that sustained hypoxia would result in the greatest …


Novel Mechanism Of Endogenous Pancreatic Cancer Cell Expression Of Immune Checkpoint Programmed Cell-Death 1 Protein (Pd-1) Inducing Epithelial-To-Mesenchymal Transition (Emt) Through The Met Pathway And Promoting Cancer Progression In An Immune-Independent Process, Megan M. Harper 2022 University of Kentucky

Novel Mechanism Of Endogenous Pancreatic Cancer Cell Expression Of Immune Checkpoint Programmed Cell-Death 1 Protein (Pd-1) Inducing Epithelial-To-Mesenchymal Transition (Emt) Through The Met Pathway And Promoting Cancer Progression In An Immune-Independent Process, Megan M. Harper

Theses and Dissertations--Clinical and Translational Science

Pancreatic ductal adenocarcinoma (PDAC) remains one of the deadliest cancers with few treatment options, necessitating an urgent need for novel therapeutics. Immuno-oncologic (IO) therapies have revolutionized anti-cancer regimens in the past decade but typically involve reactivation of adaptive immune responses. In particular, immune checkpoint PD-1 is traditionally expressed only on immune cells while PD-L1 (PD-1 ligand) is overexpressed on cancer cells. When tumor-endogenous PD-L1 binds the PD-1 receptor on T-cells, the immune cells undergo anergy resulting in self-tolerance and cancer cell immune evasion. However, contrary to standard dogma, we previously demonstrated tumor-endogenous PD-1 expression in PDAC. Our data indicated that …


Safety In The Woods: Studying The Effectiveness Of The Kentucky Master Logger Program, Austin Winn Leake 2022 University of Kentucky

Safety In The Woods: Studying The Effectiveness Of The Kentucky Master Logger Program, Austin Winn Leake

Theses and Dissertations--Community & Leadership Development

Logging is a dangerous field; in this study the researcher looks to see the knowledge both before and after professional loggers participate in the Kentucky Master Logger Program. This information was used to see the overall effectiveness of the Kentucky Master Logger Program. The researcher found an overall improvement in logging professionals’ perceptions and understanding of logging safety. The researcher recommends creating an emphasis on a safe work culture through safety incentives and a greater focus on less used personal protective equipment such as insecticide


Epidemiologia De La Tripanosomosis En Bovidos Del Departamento Del Caqueta, Diana Cristina Sánchez Arévalo 2022 Universidad de La Salle, Bogotá

Epidemiologia De La Tripanosomosis En Bovidos Del Departamento Del Caqueta, Diana Cristina Sánchez Arévalo

Maestría en Agrociencias

La tripanosomosis bovina es una enfermedad de ocurrencia global, causada por el protozoario flagelado Trypanosoma vivax que es trasmitido por la mosca tsé-tsé en África. La enfermedad se introdujo en América del Sur con la importación de ganado procedente de África Occidental a inicios del siglo XX. En el continente americano el parásito se adaptó a la transmisión mecánica por dípteros hematófagos como Stomoxys sp. y Tabanus sp. y de forma iatrogénica. Los estudios en Colombia son escasos, se sabe que el organismo circula de forma endémica en la Costa Atlántica y Llanos Orientales, Valle del Cauca y Magdalena Medio. …


Identifying And Minimizing Sources Of Variability Within Modern Spectroscopic Techniques For The Forensic Analysis Of Glass, Oriana Christy Ovide 2022 West Virginia University

Identifying And Minimizing Sources Of Variability Within Modern Spectroscopic Techniques For The Forensic Analysis Of Glass, Oriana Christy Ovide

Graduate Theses, Dissertations, and Problem Reports

Broken glass is a trace material frequently found at crime scenes such as hit-and-runs, burglaries, assaults, and homicides. Existing research encompassing the forensic analysis of glass evidence is vast. Published studies cover the analysis and interpretation of various types of glass. However, organizations such as the National Institute of Justice (NIJ), National Institute of Standards and Technology Organization of Scientific Area Committees (NIST-OSAC), and American Society of Trace Evidence Examiners (ASTEE) continue to identify glass-specific research needs to strengthen the scientific foundations of the field. Current gaps within the forensic glass community involve understanding modern glass formulations, re-evaluating the performance …


Screening Ocean Samples From Georgia For The Presence Of Msx And Dermo Using Pcr And Qpcr Methods, Olivia Michelle Williams 2022 University of New Hampshire

Screening Ocean Samples From Georgia For The Presence Of Msx And Dermo Using Pcr And Qpcr Methods, Olivia Michelle Williams

Honors Theses and Capstones

This Senior Thesis project aimed to be able to screen samples that came in from a PhD student in Georgia for the presence of two oyster diseases, MSX and Dermo. This study was done using PCR followed by visualizing on E-gels for initial presence of disease. These were then followed by qPCR and visualization on Criterion gels. These Criterion gels were able to be analyzed in order to calculate the pg/uL of MSX and Dermo in the original ocean samples. Since the samples were sent in over the course of February-August ‘21 the presence over time was also able to …


D-Dimer Testing In Covid-19: From Basics To Clinical Application, Bushra Moiz 2022 Aga Khan University

D-Dimer Testing In Covid-19: From Basics To Clinical Application, Bushra Moiz

Department of Pathology and Laboratory Medicine

No abstract provided.


Investigating The Genetic Diversity Of Immune Genes In Non-Native Populations Of American Bullfrogs (Rana Catesbeiana), Luisa Nereyda Segovia 2022 Humboldt State University

Investigating The Genetic Diversity Of Immune Genes In Non-Native Populations Of American Bullfrogs (Rana Catesbeiana), Luisa Nereyda Segovia

Cal Poly Humboldt theses and projects

The American Bullfrog (Rana catesbeiana) is a highly invasive species that has successfully colonized habitats around the world. The genetic variability of immune genes within invasive populations, like that of bullfrogs, may contribute to how resistant a population is to pathogens. The objective of this project was to characterize the genetic diversity of an immune gene in invasive bullfrog populations in California to better understand how persistent these populations might be over time. To characterize immune gene variability, I isolated exon 2 of the Major Histocompatibility Complex (MHC) Class II Beta chain gene and a neutral mitochondrial marker, …


Chronic Adolescent Stress As A Predictive Factor For The Risk Of Developing Ptsd-Like Symptoms In Adulthood, Grace K. Young 2022 Virginia Commonwealth University

Chronic Adolescent Stress As A Predictive Factor For The Risk Of Developing Ptsd-Like Symptoms In Adulthood, Grace K. Young

Theses and Dissertations

Post-traumatic stress disorder is a stress and trauma based psychological disorder that is defined by the DSM-IV as an anxiety disorder that affects approximately 7.8% of people in the United States. PTSD is when those who suffer a traumatic event have intense and distressing feelings, emotions, and memories for a prolonged period of time after the event. A prominent feature of PTSD is the impaired ability to properly extinguish a fear response after a dangerous trigger or stressor is no longer present, also known as safety learning. Stressors are threats perceived within the environment that activate a response within the …


4d Ex Vivo Crispr/Cas9 Whole-Genome Screen To Identify Genes Regulating Lung Cancer Metastasis, Alexandria Plumer 2021 The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences

4d Ex Vivo Crispr/Cas9 Whole-Genome Screen To Identify Genes Regulating Lung Cancer Metastasis, Alexandria Plumer

The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences Dissertations and Theses (Open Access)

Metastatic lung cancer has a 5-year survival rate of 5%. Lung cancers tend to be asymptomatic until late stages, and almost 90% are not diagnosed until they are advanced. Metastases are very rare events, often initiated by a single cell from a primary tumor into a new niche at a distant location. Investigation of the early metastatic process is of urgent need for the development of early diagnostics and targeted therapeutics. We performed a proof-of-concept CRISPR/Cas9 whole genome knockout screen in the A549 lung adenocarcinoma cell line and utilized a novel ex vivo 4D lung metastasis model to find gene …


Influence Of Saltwater Submergence On Geohydraulic Properties Of Sand: A Laboratory Investigation, Sudip Basack, Ghritartha Goswami, Sumanpran Sonowal, Moses Karakouzian 2021 MAKA University of Technology

Influence Of Saltwater Submergence On Geohydraulic Properties Of Sand: A Laboratory Investigation, Sudip Basack, Ghritartha Goswami, Sumanpran Sonowal, Moses Karakouzian

Civil & Environmental Engineering and Construction Faculty Publications

Saline water intrusion into freshwater aquifers is a major geohydraulic problem relevant to coastal environment. Apart from contaminating the fresh groundwater resources, the saltwater intrusion alters the geotechnical properties of the aquifer materials, affecting the coastal water resource planning and management. The present study focuses on an in-depth laboratory investigation of the influence of saltwater submergence on the geohydraulic properties of sand. The fine sand sample was submerged under saline water of specified concentrations for specific periods, and the alteration in their engineering properties has been studied. It is observed that the specific gravity, dry density, and permeability of fine …


The Role Of The Erbb Signaling Pathway In Cardiovascular Progenitor Cell-Based Repair, Christopher Ramos 2021 California State University, San Bernardino

The Role Of The Erbb Signaling Pathway In Cardiovascular Progenitor Cell-Based Repair, Christopher Ramos

Electronic Theses, Projects, and Dissertations

Adult mammalian hearts lack self-renewal and proliferative capabilities necessary for cardiovascular regeneration. Current treatments using cardiac progenitor cells (CPCs) for cell-based repair do not restore cardiac function in patients who experience a myocardial infarction (MI). Our laboratory has been studying Islet-1+ neonatal CPCs as a promising candidate for cell-based repair due to their ability to significantly improve cardiac function after MI in sheep. The current study addresses the hypothesis that the ERBB pathway is linked to the Hippo-pathway to activate YAP1 by the involvement of an autocrine loop that upregulates neuregulin (NRG). In our sheep model of MI and cardiovascular …


Laboratory Research Skills, Joanna Burkhardt 2021 University of Rhode Island

Laboratory Research Skills, Joanna Burkhardt

Library Impact Statements

No abstract provided.


Laboratory Research Skills (Permanent), Joanna Burkhardt 2021 University of Rhode Island

Laboratory Research Skills (Permanent), Joanna Burkhardt

Library Impact Statements

No abstract provided.


Reproducibility Of Alkaline Inorganic Phosphate Quantification Using 31p-Magnetic Resonance Spectroscopy At 3t, Alexs A. Matias 2021 University of Massachusetts Amherst

Reproducibility Of Alkaline Inorganic Phosphate Quantification Using 31p-Magnetic Resonance Spectroscopy At 3t, Alexs A. Matias

Masters Theses

INTRODUCTION: The detection of a second inorganic phosphate (Pi) resonance, a possible marker of mitochondrial content in vivo, using phosphorus magnetic resonance spectroscopy (31P- MRS) at 3T is technically challenging, which may prevent its reproducible quantification. PURPOSE: To determine the reproducibility of resting alkaline inorganic phosphate (Pialk) measurement using 31P-MRS in human skeletal muscle at 3 tesla (T). METHODS: Resting 31P- MRS of the quadriceps muscles was acquired on two separate visits, within seven days, in 13 healthy, sedentary to moderately active young adults using a whole-body 3T MR system. Measurement variability related to coil position, shimming procedure, and spectral …


Motor Unit Firing Rate Control Of Agonist Skeletal Muscle During Voluntary Isometric And Shortening Contractions With Limb Movement, Eric A. Kirk 2021 The University of Western Ontario

Motor Unit Firing Rate Control Of Agonist Skeletal Muscle During Voluntary Isometric And Shortening Contractions With Limb Movement, Eric A. Kirk

Electronic Thesis and Dissertation Repository

Voluntary movements throughout mammalian lifespan require skeletal muscle contractions that are controlled by time- and recruitment-dependent firing rate patterns. Single motor unit (MU) activity reflects the final efferent neural drive to the muscle, yet the underlying neural control of movement at the MU level is not well understood. Using intramuscular electromyography single MU recordings, relationships between voluntary contraction kinematics and MU firing rates were evaluated in vivo, in groups of young and older adult participants.

The purpose of study one was to characterize how MU firing rates are differently scaled among muscles relative to voluntary contraction intensity. Across 12 …


Exogenous Surfactant As A Delivery Vehicle For Intrapulmonary Therapeutics, Brandon J. Baer 2021 The University of Western Ontario

Exogenous Surfactant As A Delivery Vehicle For Intrapulmonary Therapeutics, Brandon J. Baer

Electronic Thesis and Dissertation Repository

As an organ system, the lung has unique advantages and disadvantages for direct drug delivery. Its contact with the external environment allows for the airways to be easily accessible to intrapulmonary delivery. However, its complex structure, which divides into more narrow airways with each branch, can make direct delivery to the remote alveoli challenging. The objective of this thesis was to overcome this issue by using exogenous surfactant, a lipoprotein complex used to treat neonatal respiratory distress syndrome, as a carrier for pulmonary therapeutics. It was hypothesized that therapeutics administered with a surfactant vehicle would display enhanced delivery to the …


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