Entomology Commons^™

Imaging The Oocysts In The Midgut Of The Mosquito, Hillary Guzik

Department of Entomology: Distance Master of Science Projects

● Learn how to dissect the midgut from the mosquito ○ Protocol on how to conduct the dissection ● Produce a portfolio of high end microscopy images of the oocysts inside of the midgut ○ Using Leica SP5 Point Scanning Confocal (Analytical Imaging Facility at Einstein College of Medicine) ○ Reconstruct the oocyst in 3D ○ To further be used in Dr. Kami Kim’s research ● Develop an image analysis protocol to automatically count each individual oocyst ○ Using Volocity analysis software (PerkinElmer) ○ Gather total counts and area measurements of individual oocysts ○ Written for future use ○ To …

Entomology 101: Introduction To Entomology, Brian Kelly

Department of Entomology: Distance Master of Science Projects

The goal of this particular project is to create an entry-level curriculum for Entomology to present to the local university / community college. My Bachelor’s Degree was obtained from the University of Colorado at Colorado Springs (UCCS). The higher institutions of learning in the city of Colorado Springs (to include Pikes Peak Community College, or PPCC, as well) lack any courses relating to Entomology. UCCS has a strong presence in the city and is growing by leaps and bounds, currently enrolling about 13,000 students and expanding its degree programs. PPCC has several campuses around town, and offers many elective courses …

The Biology, Ecology, And Management Of The Migratory Grasshopper, Melanoplus Sanguinipes (Fab.), Dewey W. Murray

Department of Entomology: Distance Master of Science Projects

Grasshoppers have caused significant damage to crops of farmers and rangeland forage of ranchers throughout history. Populations of grasshoppers can build and explode with exponential growth under the right climatic factors and habitat. Melanoplus sanguinipes is the most economic species of grasshopper in the United States. The migratory flight capabilities are very similar to true locusts. This species has been known to travel distances of over 500 miles. Millions of dollars in damage occurs to crops and rangeland annually in the United States due to this species and other economic grasshopper species. The life history of this species and timing …

Battle In The New World: Helicoverpa Armigera Versus Helicoverpa Zea (Lepidoptera: Noctuidae), José P. F. Bentivenha, Silvana L. Vieira De Paula Moraes, Edson L. L. Baldin, Alexandre Specht, Ivana F. Da Silva, Thomas E. Hunt

Department of Entomology: Faculty Publications

The corn earworm Helicoverpa zea (Boddie) and the old world bollworm H. armigera (HuÈbner) (Lepidoptera: Noctuidae) are allopatric species and occur in important agricultural crops. In maize, both species tend to infest the ear. The introduction of H. armigera in Brazil has created a new scenario, where these Helicoverpa species might cohabit and interact with one another, affecting the prevalence of each species in the agroecosystem, integrated pest management, and insect resistance management. In this study, larval occurrence and proportion of these species in maize was assessed in three regions of Brazil during three crop seasons. Interaction between …

Evidence Of Female Sex Pheromones And Characterization Of The Cuticular Lipids Of Unfed, Adult Male Versus Female Blacklegged Ticks, Ixodes Scapularis, Ann L. Carr, Daniel E. Sonenshine, John B. Strider Jr., R. Michael Roe

Biological Sciences Faculty Publications

Copulation in I. scapularis involves physical contact between the male and female (on or off the host), male mounting the female, insertion/maintenance of the male chelicerae in the female genital pore (initiates spermatophore production), and the transfer of the spermatophore by the male into the female genital pore. Bioassays determined that male mounting behavior/chelicerae insertion required direct contact with the female likely requiring non-volatile chemical cues with no evidence of a female volatile sex pheromone to attract males. Unfed virgin adult females and replete mated adult females elicited the highest rates of male chelicerae insertion with part fed virgin adult …

Mevalonate-Farnesal Biosynthesis In Ticks: Comparative Synganglion Transcriptomics And A New Perspective, Jiwei Zhu, Sayed M. Khalil, Robert D. Mitchell, Brooke W. Bissinger, Noble Egekwu, Daniel E. Sonenshine, R. Michael Roe

Biological Sciences Faculty Publications

Juvenile hormone (JH) controls the growth, development, metamorphosis, and reproduction of insects. For many years, the general assumption has been that JH regulates tick and other acarine development and reproduction the same as in insects. Although researchers have not been able to find the common insect JHs in hard and soft tick species and JH applications appear to have no effect on tick development, it is difficult to prove the negative or to determine whether precursors to JH are made in ticks. The tick synganglion contains regions which are homologous to the corpora allata, the biosynthetic source for JH in …

Tick Genome Assembled: New Opportunities For Research On Tick-Host-Pathogen Interactions, Jose De La Fuente, Robert M. Waterhouse, Daniel E. Sonenshine, R. Michael Roe, Jose M. Ribeiro, David B. Sattelle, Catherine A. Hill

Biological Sciences Faculty Publications

As tick-borne diseases are on the rise, an international effort resulted in the sequence and assembly of the first genome of a tick vector. This result promotes research on comparative, functional and evolutionary genomics and the study of tick-host-pathogen interactions to improve human, animal and ecosystem health on a global scale.

Eco-Friendly Nets To Improve Vegetable Production And Quality In Sub-Saharan Africa, T. Martin, S. Simon, L. Parrot, F. Assogba Komlan, F. Vidogbena, A. Adegbidi, V. Baird, M. Saidi, M. Kasina, L. A. Wasilwa, S. Subramanian, M. Ngouajio

All Peer-Reviewed Publications

Continuous coverage of vegetables with nets under different climate conditions in Benin and Kenya proved effective in protecting vegetable nurseries and main fields against key lepidopteran and dipteran pests. However, netting did not effectively protect the vegetables against small insects such as aphids and whiteflies, although a visual barrier could delay outbreaks. Hence, opening the nets during the day could be an alternative option to conserve the natural enemies for small insects. Moreover, the efficacy of the visual barrier may be complemented by a repellent compound impregnated in the net. The small-holder growers adopting the nets in Benin and Kenya …

Antagonistic Interactions Between The Africanweaver Ant Oecophylla Longinoda And The Parasitoid Anagyrus Pseudococci Potentially Limits Suppression Of The Invasive Mealybug Rastrococcus Iceryoides, Chrysantus M. Tanga, Sunday Ekesi, Prem Govender, Peterson W. Nderitu, Samira A. Mohamed

All Peer-Reviewed Publications

The ant Oecophylla longinoda Latreille forms a trophobiotic relationship with the invasive mealybug Rastrococus iceryoides Green and promotes the latter’s infestations to unacceptable levels in the presence of their natural enemies. In this regard, the antagonistic interactions between the ant and the parasitoid Anagyrus pseudococci Girault were assessed under laboratory conditions. The percentage of parasitism of R. iceryoides by A. pseudococci was significantly higher on “ant-excluded” treatments (86.6% ± 1.27%) compared to “ant-tended” treatments (51.4% ± 4.13%). The low female-biased sex-ratio observed in the “ant-tended” treatment can be attributed to ants’ interference during the oviposition phase, which disrupted parasitoids’ ability …

Onciderini Thomson, 1860 (Coleoptera: Cerambycidae: Lamiinae) Types Of The Muséum National D’Histoire Naturelle (Mnhn), With A Brief History Of The Coleoptera Collection, Eugenio H. Nearns, Gérard L. Tavakilian

Insecta Mundi

The primary types of Onciderini Thomson, 1860 of the Muséum national d’Histoire naturelle (MNHN), Paris, are catalogued and illustrated. Data on the original combination, current name, gender, and type locality are verifi ed and presented. There are 139 primary types of Onciderini including 38 in Oncideres Lacordaire, 1830; 17 in Hypsioma Audinet-Serville, 1835; 10 in Hesycha Fairmaire and Germain, 1859; nine in Hypselomus Perty, 1832; and eight each in Eudesmus Audinet-Serville, 1835 and Trestonia Buquet, 1859. Of the 139 primary types, 71 were described by J. Thomson, 34 by H. W. Bates, 13 by the authors of this work, and …

Testing The Selectivity Of Pesticide Effects On Natural Enemies In Laboratory Bioassays, Kaushalya G. Amarasekare, Peter Shearer, Nicholas J. Mills

Agricultural and Environmental Sciences Faculty Research

The toxic effects of older classes of pesticides on natural enemies are typically acute and exposure usually occurs through direct contact with foliar residues. However, older chemistries are being replaced by newer classes of pesticides that can cause sublethal effects in addition to direct mortality. We developed a set of life table response protocols to quantify the effects of multiple routes of exposure to pesticides on individual-level life history parameters of predators and parasitoids. We then integrated the data into population-level endpoint estimates of population growth rates using stage-structured population models. For this study, we evaluated the impacts of five …

Selection Of Reference Genes For Rt-Qpcr Analysis In A Predatory Biological Control Agent, Coleomegilla Maculata (Coleoptera: Coccinellidae), Chunxiao Yang, Huipeng Pan, Jeffrey Edward Noland, Deyong Zhang, Zhanhong Zhang, Yong Liu, Xuguo Zhou

Entomology Faculty Publications

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for quantifying gene expression across various biological processes, of which requires a set of suited reference genes to normalize the expression data. Coleomegilla maculata (Coleoptera: Coccinellidae), is one of the most extensively used biological control agents in the field to manage arthropod pest species. In this study, expression profiles of 16 housekeeping genes selected from C. maculata were cloned and investigated. The performance of these candidates as endogenous controls under specific experimental conditions was evaluated by dedicated algorithms, including geNorm, Normfinder, BestKeeper, and ΔCt method. In addition, …

L3 Auditory Interneuron Responses To Dual-Frequency Calling Songs In Female Crickets (Acheta Domesticus), Jeong Bin Lee

Honors Theses

Female crickets exhibit positive phonotaxis in response to calling songs with specific carrier frequencies (4-5 kHz), syllable periods (SP; 50-70ms), and other parameters. Among a number of ascending prothoracic auditory neurons identified, only L3 has been proposed to be involved in SP selective phonotaxis. L3 responds with selective decrement to 5 kHz stimulus with attractive SPs. Furthermore, L3 is highly tuned to 16 kHz auditory stimulus, which has been correlated with aversive phonotactic behaviors. L3’s cellular responses to simultaneous 16 kHz and 5 kHz stimuli were examined through extracellular recording, thereby further establishing the connection between L3’s selective decrement and …

Survey For Spotted-Wing Drosophila (Diptera: Drosophilidae) In The Five-County Nursery Production Region Of Middle Tennessee, Usa, Karla M. Addesso, Jason B. Oliver, Paul A. O'Neal

Agricultural and Environmental Sciences Faculty Research

Through global trade, spotted-wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), a native of Southeast Asia, has spread to at least 3 other continents since 2008. Initial reports of the fly in new regions often are associated with damage in agriculture fields, but the fly may be present in the landscape for years before populations reach a level at which they become a pest in fruit production. In 2012, spotted wing drosophila was reported in blueberry fields in eastern Tennessee, USA, for the first time. In order to determine whether the fly was established in middle Tennessee, we conducted landscape surveys …

Cretonthophilus Tuberculatus , A Remarkable New Genus And Species Of Hister Beetle (Coleoptera: Histeridae) From Cretaceous Burmese Amber, Michael S. Caterino, Karin Wolf-Schwenninger, Gunter Bechly

Publications

The early history of the beetle family Histeridae is still very obscure. In part this results from difficulty resolving phylogenetic relationships at deeper levels (Caterino & Vogler, 2002; McKenna et al., 2015a). But it is also partly a result of a sparse and poorly documented fossil record (Chatzimanolis et al., 2006). Here we describe a new genus and species of fossil histerid from Burmese amber (~99 mya), which helps to address both of these problems. Although histerid fossils have been reported from a variety of fossil localities and strata, relatively few have been adequately described. Until recently, the earliest described …

A Comparative Study Of Medicago Truncatula Transcriptomes As They Relate To Saponin Defenses Against Insect Pests, Audra Mae Rogers

Graduate Theses and Dissertations

Triterpene saponins are a class of defensive plant-derived compounds, which have a

variety of functions including antimicrobial, insecticidal, and nematicidal activities. This

research assessed the performance of the beet armyworm, Spodoptera exigua, following ad

libero feeding on Medicago truncatula accessions differing in specialized metabolite and

saponin profiles. Insect performance did not directly relate to the foliar saponin levels in the

accessions, which was measured using HPLC mass spectrometry. Accession ESP105 had the lowest foliar saponins overall, yet had similar levels of soyasaponins compared to the other accessions. Conversely, accessions A17, PRT178, and GRC43 had high foliar saponins with particularly high …

Field Abundance And Performance Of Hoverflies (Diptera: Syrphidae) On Soybean Aphid, James O. Eckberg, Julie A. Peterson, Colin P. Borsh, Joe M. Kaser, Gregg A. Johnson, John C. Luhman, Donald L. Wyse, George E. Heimpel

West Central Research and Extension Center, North Platte

The management of the soybean aphid Aphis glycines Matsumara is a major challenge to soybean production in the north-central United States. The identification and characterization of the insect predator community has informed integrated pest management strategies by providing insight on predators that can suppress soybean aphid populations. Hoverflies (Diptera: Syrphidae) are known predators of A. glycines, but more information is needed on their diversity, abundance, and performance to evaluate their importance as biological control agents of A. glycines. In this study, syrphid abundance was evaluated across two growing seasons in four soybean fields in east-central Minnesota. Six methods …

Biotic Potential And Reproductive Parameters Of Spodoptera Dolichos (Lepidoptera: Noctuidae) In The Laboratory, Débora Goulart Montezano, Daniel R. Sosa-Gómez, Silvana L. Vieira De Paula Moraes, Vânia F. Roque-Specht, Edegar Fronza, Neiva M. Barros, Alexandre Specht

Department of Entomology: Faculty Publications

The biotic potential and reproductive parameters of Spodoptera dolichos (Fabricius, 1794) were evaluated under controlled conditions (25 ± 1°C, 70 ± 10% RH and 14 hour photophase). The longevity, pre-, post- and oviposition periods, fecundity, and fertility of 25 couples were evaluated. The longevity of females (12.9 days) was not significantly different than that of males (12.4 days). The mean durations of the pre-, post- and oviposition periods were 3.0, 0.4 and 10.4 days, respectively. The mean fecundity was 4,086.0 eggs per female and mean fertility was 3,557.8 larvae per female. On average, a female copulated 1.4 times. The biotic …

Hexapod Herald - Vol. 27, No. 5, December 2015

Hexapod Herald and Other Entomology Department Newsletters

Contents: Welcome - Congratulations - Student News - Faculty News - Grants - Publications

Systematics Of Testudacarine Torrent Mites (Parasitengona: Torrenticolidae), Joseph Charles O'Neill

Graduate Theses and Dissertations

Thirteen new species of Testudacarus (Torrenticolidae: Testudacarinae) are described, four species are redescribed, and the status of previously problematic species are addressed. For Testudacarinae this represents the first published: 1) descriptions from multiple specimens (therefore providing ranges); 2) colored photographs; 3) explicit illustrations and discussion of sexual dimorphism within the subfamily; 4) genetic data. A comprehensive literature review is also included.