Entomology Commons^™

High-Throughput Transcriptome Sequencing For Snp And Gene Discovery In A Moth, Nicholas J. Miller, Jing Sun, Thomas W. Sappington

Department of Entomology: Faculty Publications

The western bean cutworm, Striacosta albicosta (Smith) (Leptidoptera: Noctuidae) is a pest of corn (Zea mays L.) and dry beans that underwent a dramatic range expansion in North America during the first decade of the 21st century. Research into the population genetics of this species has been hindered by a lack of genetic markers. The transcriptome of adult male S. albicosta was partially sequenced using Illumina sequencing-by-synthesis. Assembly of the sequence reads yielded 16,847 transcript sequences, of which 6,631 could be assigned a putative function. A search for single nucleotide polymorphisms (SNPs) identified 2,487 candidate SNPs distributed among 1,265 …

Trapping And Control Of The Small Hive Beetle, Aethina Tumida, An Invasive Parasite Of Honey Bees, Apis Mellifera, Shannon Peterson

All Theses

The small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is one of the most recent honey bee pests of economic importance, especially in the southeastern United States. Various in-hive traps have been developed to attempt to control the populations of this invasive pest within honey bee colonies. The first year of my research focused on comparing the effectiveness of three commercially available traps for removing small hive beetles. Thirty-two colonies were established with 0.9-kg package bees with a queen in four apiaries. Eight colonies were placed in each apiary, each colony randomly receiving one of four treatments: the three-chambered Hood …

Chromosomal Patterns In Populations Of The Black Fly Simulium Fibrinflatum Twinn (Diptera: Simuliidae), Kyle Parks

All Theses

Cryptic species are an important component of biodiversity, although most cryptic species have yet to be discovered. Cytogenetic studies of polytene chromosomes offer a unique level of resolution for detecting cryptic species in the family Simuliidae. Simulium fibrinflatum is a black fly morphospecies that is suspected to contain cryptic species because of its broad geographic range and variable pupal gill structure. Populations of Simulium fibrinflatum from the Saugahatchee River, AL; Anthony Shoals, GA; LaHave River, Nova Scotia; Westfield River, MA; Chauga River, SC; and Enoree River, SC, were sampled and cytogenetically analyzed to test for the presence of cryptic species. …

Fluid Uptake By The Lepidopteran Proboscis In Relation To Structure, Karena Kwauk

All Theses

Three species of butterflies--eastern tiger swallowtail (Papilio glaucus L.), monarch (Danaus plexippus L.), and painted lady (Vanessa cardui L.)--were used to investigate the effect that exposing multiple regions of the proboscis to fluid has on feeding rate. Although the lepidopteran proboscis historically has been considered a sealed tube with a specialized region near the tip for fluid uptake, droplets of 1% sucrose solution were shown to enter multiple locations along the proboscis. Feeding experiments were conducted comparing fluid uptake rates of butterflies with their proboscises either fully or partially submerged in 1% or 15% sucrose solutions. Only one significant relationship …

Efficacy Of Precision-Applied In-Furrow Insecticide/Nematicide And Seed Treatments On Populations Of Thrips And Nematodes In Cotton, Ginger Devinney

All Theses

Thrips and nematodes can be serious early-season pests of cotton grown on the highly variable soil types common in the southeastern United States. I examined the effects of precision-applied aldicarb (Temik¨ 15G) and seed treatments (Aeris¨ and Avicta Complete Pak¨) on population dynamics of thrips and nematodes in management zones defined by soil texture as measured by soil electrical conductivity (EC). Research plots were established in cotton fields with variable soil types at the Clemson University Edisto Research and Education Center near Blackville, SC, during May of 2010 and 2011. Research plots were arranged in a three-way factorial design, with …

Ecology And Phylogeny Of The Biting-Midge Genus Culicoides (Diptera: Ceratopogonidae), Dustin Swanson

All Dissertations

Flies of the genus Culicoides are small, biting midges in the family Ceratopogonidae. These flies are of medical, veterinary, and economic importance because their blood-feeding behavior can cause stress to hosts and transmit disease agents. Despite the importance of these flies, little is known about their biology, especially the ecology of the immature stages and the phylogenetic relationships among taxa. The objectives of my study were to address these two areas of Culicoides biology.
Larval Culicoides were collected from aquatic habitats in four ecoregions of South Carolina, USA. Eleven ecological variables were recorded for each sample. Larvae were identified by …

Flight Period And Species Composition Of Sirex (Hymenoptera: Siricidae) And Associated Deladenus (Nematoda: Neotylenchidae) Within Arkansas Pine Forests, Danielle Keeler

Graduate Theses and Dissertations

The European woodwasp, Sirex noctilio F. (Hymenoptera: Siricidae), which is a known destructive pest of pine in the southern hemisphere was recently discovered in the eastern United States. Before we can understand how S. noctilio may affect pine forests throughout the United States, we need a better understanding of native Sirex and the role they play in the ecosystem. The objectives of this research were to 1) determine species composition and flight period for native Sirex; 2) confirm presence of parasitic nematodes, Deladenus (Nematoda: Neotylenchidae), within adult Sirex; 3) investigate Deladenus parasitism rates; 4) verify the number of Deladenus species …

Surveillance Of Mosquitoes For Arthropod-Borne Viruses In El Paso, Tx., Stephanie Coronado, Douglas Watts

COURI Symposium Abstracts, Summer 2012

West Nile encephalitis is the only arthropod-borne virus that causes annual cases of human illness in El Paso, Texas. Cases more commonly affect the young and elderly and the illness may range from a mild febrile disease to fatal encephalitis. The primary vector(s) of West Nile virus are Culex species mosquitoes, including Culex tarsalis and Culex quinquefaciatus. Further, surveillance is needed to determine the relative importance of these and possibly other mosquito species. More recently, cases of dengue fever and fatal hemorrhagic fever have been occurring in communities along the USA-Mexico border. Dengue has been endemic in Mexico since the …

Molecular Identification Of Synanthedonini Members (Lepidoptera: Sesiidae) Using Cytochrome Oxidase I, Jason A. Hansen, William E. Klingeman, J. Kevin Moulton, Jason B. Oliver, Mark T. Windham, Aijun Zhang, Robert N. Trigiano

Agricultural and Environmental Sciences Faculty Research

Many North American sesiid moths within Synanthodonini have been studied extensively because their feeding activity can cause detrimental economic and esthetic impacts to many commercially important ornamental and native plant species. Recent discoveries of nonnative clearwing moth pest introductions [e.g., Synanthedon myopaeformis (Borkh.)], reinforce the need for reliable and accurate molecular diagnostic tools that can be used by nontaxonomic experts, particularly when juvenile life stages are recovered from infested host-plant tissues. Cytochrome oxidase I (cox I) previously has been used to successfully identify species and resolve species complexes. In this study, the cox I phylogeny inferred from sequences generated from …

A New Species Of Saphonecrus (Hymenoptera, Cynipoidea) Associated With Plant Galls On Castanopsis (Fagaceae) In China, Zhiwei Liu, Xiao-Hui Yang, Dao-Hong Zhu, Yi-Yuan He

Faculty Research & Creative Activity

A new cynipid species, Saphonecrus hupingshanensis Liu, Yang, et Zhu, sp. nov. (Hymenoptera: Cynipidae: Synergini), is described from China. This is the first species of the inquilinous tribe Synergini ever known to have an association with chinquapins (Fagaceae: Castanopsis). The biology and implication to species diversity of Cynipidae in eastern and southeast Asia are discussed.

A New Species Of Saphonecrus (Hymenoptera, Cynipoidea) Associated With Plant Galls On Castanopsis (Fagaceae) In China, Zhiwei Liu, Xiao-Hui Yang, Dao-Hong Zhu, Yi-Yuan He

Faculty Research & Creative Activity

A new cynipid species, Saphonecrus hupingshanensis Liu, Yang, et Zhu, sp. nov. (Hymenoptera: Cynipidae: Synergini), is described from China. This is the first species of the inquilinous tribe Synergini ever known to have an association with chinquapins (Fagaceae: Castanopsis). The biology and implication to species diversity of Cynipidae in eastern and southeast Asia are discussed.

Penetration Of Termiticide Treatments Into Gravel Used As A Construction Fill Material, Chris J. Peterson

Midsouth Entomologist

The penetration of five termiticidal suspensions (Termidor, Premise, Talstar, Phantom, and Transport) was examined in packed columns in the laboratory, in which each column incorporated a top gravel layer and a lower soil layer. This simulates common building practices in some parts of the United States. The highest doses were usually found at the top of the soil layer beneath the gravel, indicating that the applied suspension (at either the normal labeled volume or a higher volume allowed for treating gravel) penetrated to the soil below at levels sufficient to kill termites (Isoptera: Rhinotermitidae). The active ingredients were retained by …

Insect Succession On Pig Carrion In North-Central Mississippi, J. Goddard, D. Fleming, J. L. Seltzer, S. Anderson, C. Chesnut, M. Cook, E. L. Davis, B. Lyle, S. Miller, E. A. Sansevere, W. Schubert

Midsouth Entomologist

A freshly-euthanized 90 kg Yucatan mini pig, Sus scrofa domesticus, was placed outdoors on 21 March 2012, at the Mississippi State University South Farm and two teams of students from the Forensic Entomology class were assigned to take daily (weekends excluded) environmental measurements and insect collections at each stage of decomposition until the end of the semester (42 days). Assessment of data from the pig revealed a successional pattern similar to that previously published – fresh, bloat, active decay, and advanced decay stages (the pig specimen never fully entered a dry stage before the semester ended). Over 2,300 immature and …

Nectar Plants Utilized By Butterflies At Trail Of Tears State Park, Missouri, Mike Smart, Kent Fothergill, Christopher D. Crabtree

Midsouth Entomologist

No abstract provided.

The Phylogeny And Classification Of Embioptera (Insecta), Janice Edgerly-Rooks, Kelly Miller, Cheryl Hayashi, Michael Whiting, Gavin Svenson

Biology

A phylogenetic analysis of the order Embioptera is presented with a revised classification based on results of the analysis. Eighty-two species of Embioptera are included from all families except Paedembiidae Ross and Embonychidae Navás. Monophyly of each of the eight remaining currently recognized families is tested except Andesembiidae Ross, for which only a single species was included. Nine outgroup taxa are included from Blattaria, Grylloblattaria, Mantodea, Mantophasmatodea, Orthoptera, Phasmida and Plecoptera. Ninety-six morphological characters were analysed along with DNA sequence data from the five genes 16S rRNA, 18S rRNA, 28S rRNA, cytochrome c oxidase I and histone III. Data were …

Nebline, July 2012

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Food Booth at Super Fair Volunteers Have Fun, Rewarding Experience

Farm Views

Urban Agriculture

Food & Fitness

Home & Family Living

Horticulture

Environmental Focus

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Bark Beetles, Ryan S. Davis, Darren Mcavoy

All Current Publications

Bark beetles are one of the most destructive forest pests in the world. They are different than the larger longhorned and roundheaded/metallic woodboring beetles commonly infesting the inner wood of trees. The largest bark beetle, the red turpentine beetle (Dendroctonus valens), reaches only 8.3 mm in length. Because of their tiny size, bark beetles are not effective tree killers as individuals.

Seasonal Photoperiods Alter Developmental Time And Mass Of An Invasive Mosquito, Aedes Albopictus (Diptera: Culicidae), Across Its North-South Range In The United States, Steven A. Juliano, Donald A. Yee, Steven M. Vamosi

Faculty Publications – Biological Sciences

The Asian tiger mosquito, Aedes albopictus (Skuse), is perhaps the most successful invasive mosquito species in contemporary history. In the United States, Ae. albopictus has spread from its introduction point in southern Texas to as far north as New Jersey (i.e., a span of approximate to 14 degrees latitude). This species experiences seasonal constraints in activity because of cold temperatures in winter in the northern United States, but is active year-round in the south. We performed a laboratory experiment to examine how life-history traits of Ae. albopictus from four populations (New Jersey [39.4 degrees N], Virginia [38.6 degrees N], North …

A Century And A Half Of Research On The Stable Fly, Stomoxys Calcitrans (L.) (Diptera: Muscidae), 1862-2011: An Annotated Bibliography, K. M. Kneeland, Steven R. Skoda, Jerome A. Hogsette, A. Y. Li, Jaime Molina-Ochoa, Kimberly Hutchinson Lohmeyer, John E. Foster

Department of Entomology: Faculty Publications

The stable fly, Stomoxys calcitrans, is a cosmopolitan pest of livestock, wild animals, pets, and humans. It is a primary pest of cattle in the United States, estimated to cause more than $1 billion in economic losses annually. It also causes dissension at the rural-urban interface and is a problem in recreation areas such as Florida beaches and the Great Lakes. Due to its pestiferous nature and painful bite, methods to control stable flies have been investigated for over a century. A large amount of research has been reported on stable fly biology, ecology, genetics, physiology, and vector competence. …

Wing Shape As An Indicator Of Larval Rearing Conditions For Aedes Albopictus And Aedes Aegypti (Diptera: Culicidae), C R. Stephens, Steven A. Juliano

Faculty Publications – Biological Sciences

Estimating a mosquito's vector competence, or likelihood of transmitting disease, if it takes an infectious bloodmeal, is an important aspect of predicting when and where outbreaks of infectious diseases will occur. Vector competence can be affected by rearing temperature and inter- and intraspecific competition experienced by the individual mosquito during its larval development. This research investigates whether a new morphological indicator of larval rearing conditions, wing shape, can be used to distinguish reliably temperature and competitive conditions experienced during larval stages. Aedes albopictus (Skuse) and Aedes aegypti (L.) (Diptera: Culicidae) larvae were reared in low intraspecific, high intraspecific, or high …