Entomology Commons^™

Parental Rnai Suppression Of Hunchback Gene To Control Hemipteran Pests, Blair D. Siegfried, Kenneth E. Narva, Kanika Arora, Sarah E. Worden, Chitvan Khajuria, Elane Fishilevich, Nicholas P. Storer, Meghan Frey, Ronda L. Hamm, Ana Velez

Department of Entomology: Faculty Publications

This disclosure concerns nucleic acid molecules and methods of use thereof for control of hemipteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in hemipteran pests. The dis closure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of hemipteran pests, and the plant cells and plants obtained thereby.

Onciderini Thomson, 1860 (Coleoptera: Cerambycidae: Lamiinae) Types Of The Muséum National D’Histoire Naturelle (Mnhn), With A Brief History Of The Coleoptera Collection, Eugenio H. Nearns, Gérard L. Tavakilian

Insecta Mundi

The primary types of Onciderini Thomson, 1860 of the Muséum national d’Histoire naturelle (MNHN), Paris, are catalogued and illustrated. Data on the original combination, current name, gender, and type locality are verifi ed and presented. There are 139 primary types of Onciderini including 38 in Oncideres Lacordaire, 1830; 17 in Hypsioma Audinet-Serville, 1835; 10 in Hesycha Fairmaire and Germain, 1859; nine in Hypselomus Perty, 1832; and eight each in Eudesmus Audinet-Serville, 1835 and Trestonia Buquet, 1859. Of the 139 primary types, 71 were described by J. Thomson, 34 by H. W. Bates, 13 by the authors of this work, and …

Testing The Selectivity Of Pesticide Effects On Natural Enemies In Laboratory Bioassays, Kaushalya G. Amarasekare, Peter Shearer, Nicholas J. Mills

Agricultural and Environmental Sciences Faculty Research

The toxic effects of older classes of pesticides on natural enemies are typically acute and exposure usually occurs through direct contact with foliar residues. However, older chemistries are being replaced by newer classes of pesticides that can cause sublethal effects in addition to direct mortality. We developed a set of life table response protocols to quantify the effects of multiple routes of exposure to pesticides on individual-level life history parameters of predators and parasitoids. We then integrated the data into population-level endpoint estimates of population growth rates using stage-structured population models. For this study, we evaluated the impacts of five …

L3 Auditory Interneuron Responses To Dual-Frequency Calling Songs In Female Crickets (Acheta Domesticus), Jeong Bin Lee

Honors Theses

Female crickets exhibit positive phonotaxis in response to calling songs with specific carrier frequencies (4-5 kHz), syllable periods (SP; 50-70ms), and other parameters. Among a number of ascending prothoracic auditory neurons identified, only L3 has been proposed to be involved in SP selective phonotaxis. L3 responds with selective decrement to 5 kHz stimulus with attractive SPs. Furthermore, L3 is highly tuned to 16 kHz auditory stimulus, which has been correlated with aversive phonotactic behaviors. L3’s cellular responses to simultaneous 16 kHz and 5 kHz stimuli were examined through extracellular recording, thereby further establishing the connection between L3’s selective decrement and …

Selection Of Reference Genes For Rt-Qpcr Analysis In A Predatory Biological Control Agent, Coleomegilla Maculata (Coleoptera: Coccinellidae), Chunxiao Yang, Huipeng Pan, Jeffrey Edward Noland, Deyong Zhang, Zhanhong Zhang, Yong Liu, Xuguo Zhou

Entomology Faculty Publications

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for quantifying gene expression across various biological processes, of which requires a set of suited reference genes to normalize the expression data. Coleomegilla maculata (Coleoptera: Coccinellidae), is one of the most extensively used biological control agents in the field to manage arthropod pest species. In this study, expression profiles of 16 housekeeping genes selected from C. maculata were cloned and investigated. The performance of these candidates as endogenous controls under specific experimental conditions was evaluated by dedicated algorithms, including geNorm, Normfinder, BestKeeper, and ΔCt method. In addition, …

Survey For Spotted-Wing Drosophila (Diptera: Drosophilidae) In The Five-County Nursery Production Region Of Middle Tennessee, Usa, Karla M. Addesso, Jason B. Oliver, Paul A. O'Neal

Agricultural and Environmental Sciences Faculty Research

Through global trade, spotted-wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), a native of Southeast Asia, has spread to at least 3 other continents since 2008. Initial reports of the fly in new regions often are associated with damage in agriculture fields, but the fly may be present in the landscape for years before populations reach a level at which they become a pest in fruit production. In 2012, spotted wing drosophila was reported in blueberry fields in eastern Tennessee, USA, for the first time. In order to determine whether the fly was established in middle Tennessee, we conducted landscape surveys …

Cretonthophilus Tuberculatus , A Remarkable New Genus And Species Of Hister Beetle (Coleoptera: Histeridae) From Cretaceous Burmese Amber, Michael S. Caterino, Karin Wolf-Schwenninger, Gunter Bechly

Publications

The early history of the beetle family Histeridae is still very obscure. In part this results from difficulty resolving phylogenetic relationships at deeper levels (Caterino & Vogler, 2002; McKenna et al., 2015a). But it is also partly a result of a sparse and poorly documented fossil record (Chatzimanolis et al., 2006). Here we describe a new genus and species of fossil histerid from Burmese amber (~99 mya), which helps to address both of these problems. Although histerid fossils have been reported from a variety of fossil localities and strata, relatively few have been adequately described. Until recently, the earliest described …

Environmental Impacts And Carry-Over Effects In Complex Life Cycles: The Role Of Different Life History Stages, Nnaemeka F. Ezeakacha

Dissertations

A challenge faced by organisms with complex life cycles is how environmental factors experienced at an earlier life stage affect the fitness at that stage or are carried over to subsequent life stages. I used container-inhabiting mosquitoes: Aedes albopictus, Aedes aegypti, Aedes triseriatus and Culex quinquefasciatus to study the interactions and performance of life history stages under specific environmental factors. I investigated the effects of egg-desiccation tolerance on egg viability and larval performance in the Aedes mosquitoes. I found increase in egg hatch rate with relative humidity and interaction between relative humidity and egg storage period. Larval performance …

A Comparative Study Of Medicago Truncatula Transcriptomes As They Relate To Saponin Defenses Against Insect Pests, Audra Mae Rogers

Graduate Theses and Dissertations

Triterpene saponins are a class of defensive plant-derived compounds, which have a

variety of functions including antimicrobial, insecticidal, and nematicidal activities. This

research assessed the performance of the beet armyworm, Spodoptera exigua, following ad

libero feeding on Medicago truncatula accessions differing in specialized metabolite and

saponin profiles. Insect performance did not directly relate to the foliar saponin levels in the

accessions, which was measured using HPLC mass spectrometry. Accession ESP105 had the lowest foliar saponins overall, yet had similar levels of soyasaponins compared to the other accessions. Conversely, accessions A17, PRT178, and GRC43 had high foliar saponins with particularly high …

Field Abundance And Performance Of Hoverflies (Diptera: Syrphidae) On Soybean Aphid, James O. Eckberg, Julie A. Peterson, Colin P. Borsh, Joe M. Kaser, Gregg A. Johnson, John C. Luhman, Donald L. Wyse, George E. Heimpel

West Central Research and Extension Center, North Platte

The management of the soybean aphid Aphis glycines Matsumara is a major challenge to soybean production in the north-central United States. The identification and characterization of the insect predator community has informed integrated pest management strategies by providing insight on predators that can suppress soybean aphid populations. Hoverflies (Diptera: Syrphidae) are known predators of A. glycines, but more information is needed on their diversity, abundance, and performance to evaluate their importance as biological control agents of A. glycines. In this study, syrphid abundance was evaluated across two growing seasons in four soybean fields in east-central Minnesota. Six methods …

Biotic Potential And Reproductive Parameters Of Spodoptera Dolichos (Lepidoptera: Noctuidae) In The Laboratory, Débora Goulart Montezano, Daniel R. Sosa-Gómez, Silvana L. Vieira De Paula Moraes, Vânia F. Roque-Specht, Edegar Fronza, Neiva M. Barros, Alexandre Specht

Department of Entomology: Faculty Publications

The biotic potential and reproductive parameters of Spodoptera dolichos (Fabricius, 1794) were evaluated under controlled conditions (25 ± 1°C, 70 ± 10% RH and 14 hour photophase). The longevity, pre-, post- and oviposition periods, fecundity, and fertility of 25 couples were evaluated. The longevity of females (12.9 days) was not significantly different than that of males (12.4 days). The mean durations of the pre-, post- and oviposition periods were 3.0, 0.4 and 10.4 days, respectively. The mean fecundity was 4,086.0 eggs per female and mean fertility was 3,557.8 larvae per female. On average, a female copulated 1.4 times. The biotic …

Hexapod Herald - Vol. 27, No. 5, December 2015

Hexapod Herald and Other Entomology Department Newsletters

Contents: Welcome - Congratulations - Student News - Faculty News - Grants - Publications

Southeastern Monochamus And Their Interactions With Healthy Shortleaf Pine Trees And Associated Ips Grandicollis Bark Beetles, Matthew Walker Ethington

Graduate Theses and Dissertations

Insects in the genus Monochamus are medium to large-sized, wood-boring beetles whose primary hosts in the Northern Hemisphere are pine trees. These beetles interact with both conifer hosts and associated insects throughout their life history. Past research has demonstrated that Monochamus are saprophagic, but recent findings show that they may colonize healthy pine trees. To determine if southeastern Monochamus could colonize healthy pines, adult Monochamus were attracted to healthy shortleaf pine trees from May to September, 2014, using host volatiles, Ips bark beetle kairomones, and congeneric pheromones. Subsequent development of oviposited eggs from 18 host trees was monitored. The results …

Sampling Terrestrial Arthropod Biodiversity: A Case Study In Arkansas, Michael Joseph Skvarla

Graduate Theses and Dissertations

The Interior Highlands is a biodiversity hotspot, with at least 200 known endemic species, but is understudied compared to hotspots, such as the Southern Appalachians. In order to begin to rectify this issue, a nine month study was conducted from mid-March through early December at a 4 ha site at Steel Creek, Buffalo National River, in Newton County, Arkansas. Thirteen collecting methods were employed, including three colors of Lindgren funnel trap, five colors of pan trap, Malaise traps, canopy traps with upper and lower collectors, pitfall traps, and Berlese-Tullgren extraction of leaf litter, which resulted in the collection of 1311 …

Stink Bugs: Spatial Distribution, Pecan Phenological Susceptibility And Sampling Program, Brian Cowell

Graduate Theses and Dissertations

An effective management program for stink bugs (SBs) in pecan groves requires knowledge of: stages of the pecans susceptible to SB damage; strata of the tree with SB damaged nuts; a practical SB monitoring method; and, effects of landscapes contributing SBs into pecan groves.

Stink bugs produced feeding punctures in pecan shucks at all phenological stages. Pecans punctured before the dough stage drop from the tree. Kernel damage occurs in the pecans’ dough stage, whereas mature pecans are not damaged.

The pecans collected from the whole tree using the tree shaker had significantly less punctures than the pecans collected from …

An Inventory Of Endemic Leaf Litter Arthropods Of Arkansas With Emphasis On Certain Insect Groups And Diplopoda, Derek Alan Hennen

Graduate Theses and Dissertations

Endemic arthropods of Arkansas were sampled and their nomenclature and distributions were updated. The Arkansas endemic species list is updated to 121 species, including 16 species of millipedes. A study of the millipedes of Arkansas was undertaken, and resulted in the first checklist and key to all millipede species in the state. 68 species are known from Arkansas, including the genera Cylindroiulus and Polydesmus. The first state records for Ophyiulus pilosus, Cylindroiulus sp., and Ptyoiulus coveanus are reported, and new county records are reported for 16 species. This represents the first key to a state's species since 1980, and the …

Systematics Of Testudacarine Torrent Mites (Parasitengona: Torrenticolidae), Joseph Charles O'Neill

Graduate Theses and Dissertations

Thirteen new species of Testudacarus (Torrenticolidae: Testudacarinae) are described, four species are redescribed, and the status of previously problematic species are addressed. For Testudacarinae this represents the first published: 1) descriptions from multiple specimens (therefore providing ranges); 2) colored photographs; 3) explicit illustrations and discussion of sexual dimorphism within the subfamily; 4) genetic data. A comprehensive literature review is also included.

Canola: A Modern Crop For A Modern Era, Kenneth J. Roché

Doctor of Plant Health Program: Dissertations and Student Research

The University of Nebraska-Lincoln Doctor of Plant Health program is a professional doctorate program with a comprehensive approach to plants and agriculture. The program emphasizes a broad interdisciplinary education across all plant-related disciplines, practical learning, research, and experience through internships. For my final required internship, I worked as a senior agricultural research intern with Research Designed for Agriculture (RD4AG) in Montana. RD4AG is a contract research organization based in Yuma, AZ with over thirty-years of experience. During my three month internship at RD4AG in Montana, a large portion of my responsibilities centered on managing regulated canola trials that were undertaken …

Evidence Of Field-Evolved Resistance To Bifenthrin In Western Corn Rootworm (Diabrotica Virgifera Virgifera Leconte) Populations In Western Nebraska And Kansas, Adriano E. Pereira, Haichuan Wang, Sarah N. Zukoff, Lance Meinke, B. Wade French, Blair Siegfried

Department of Entomology: Faculty Publications

Pyrethroid insecticides have been used to control larvae or adults of the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, a key pest of field corn in the United States. In response to reports of reduced efficacy of pyrethroids in WCR management programs in southwestern areas of Nebraska and Kansas the present research was designed to establish a baseline of susceptibility to the pyrethroid insecticide, bifenthrin, using susceptible laboratory populations and to compare this baseline with susceptibility of field populations. Concentration-response bioassays were performed to estimate the baseline susceptibility. From the baseline data, a diagnostic concentration (LC99) was determined and …

Biosafety Research For Non-Target Organism Risk Assessment Of Rnai-Based Ge Plants, Andrew F. Roberts, Yann Devos, Godwin N. Y. Lemgo, Xuguo Zhou

Entomology Faculty Publications

RNA interference, or RNAi, refers to a set of biological processes that make use of conserved cellular machinery to silence genes. Although there are several variations in the source and mechanism, they are all triggered by double stranded RNA (dsRNA) which is processed by a protein complex into small, single stranded RNA, referred to as small interfering RNAs (siRNA) with complementarity to sequences in genes targeted for silencing. The use of the RNAi mechanism to develop new traits in plants has fueled a discussion about the environmental safety of the technology for these applications, and this was the subject of …