Purification And Characterization Of Cytoplasmic Nadp+-
Isocitrate Dehydrogenase, And Amplification Of The Nadp+-Idh Gene From The Wing-Dimorphic Sand Field Cricket, Gryllus Firmus,
2010
University of Nebraska - Lincoln
Purification And Characterization Of Cytoplasmic Nadp+- Isocitrate Dehydrogenase, And Amplification Of The Nadp+-Idh Gene From The Wing-Dimorphic Sand Field Cricket, Gryllus Firmus, Anthony J. Zera, Susan Newman, David Berkheim, Christine Black, Lindsay Klug, Erica Crone
School of Biological Sciences: Faculty Publications
Cytoplasmic NADP+-isocitrate dehydrogenase (NADP+-IDH) has been purified and characterized, and its gene sequenced in many animal, plant, and yeast species. However, much less information is available on this enzyme-gene in insects. As a first step in investigating the biochemical and molecular mechanisms by which NADP+-IDH contributes to adaptations for flight vs. reproduction in insects, the enzyme was purified to homogeneity in the wing-dimorphic cricket, Gryllus firmus, characterized, and its corresponding gene sequenced. Using a combination of polyethylene glycol precipitation, Cibacron-Blue affinity chromatography, and hydrophobic interaction chromatography the enzyme was purified 291-fold (7% yield; specific activity = 15.8 μmol NADPH/min/mg …
The Transition Zone: Impact Of Riverbanks On Emergent Dragonfly Nymphs. Implications For Riverbank Restoration And Management,
2010
Antioch University of New England
The Transition Zone: Impact Of Riverbanks On Emergent Dragonfly Nymphs. Implications For Riverbank Restoration And Management, Kirsten Hope Martin
Antioch University Dissertations & Theses
The use of riprap in the restoration and stabilization of riverine landscapes is an issue of concern for many ecologists. While current methods of bank stabilization, especially those involving the placement of rocks (riprap) along the waterline, are effective in controlling erosion their presence changes habitat components (slope, substrate composition, near-shore river velocity) at the river-land interface. The additional impacts of river current, water temperature, soil composition, slope, and water level fluctuation, may further imperil emerging nymphs. The purpose of this research is to document the effects of riprap, location (upriver or downriver of hydroelectric intake/outtake facilities), water level fluctuation, …
