Entomology Commons^™

Determining The Prevalence And Distribution Of Tick-Borne Pathogens In Southeastern Virginia And Exploring The Transmission Dynamics Of Rickettsia Parkeri In Amblyomma Maculatum, Chelsea L. Wright Thompson

Biological Sciences Theses & Dissertations

Tick-borne pathogens are an increasing threat to human and animal health worldwide. In the United States, cases of Lyme disease, spotted fever rickettsioses, ehrlichiosis and anaplasmosis are on the rise. Factors related to emergence include appearance of a new pathogen, recognition of an existing pathogen and environmental changes that result in new exposure events. Despite the rise in tick-borne disease incidence within many states, including Virginia, there is a paucity of data related to the prevalence and distribution of ticks and tick-borne pathogens.

The first aim of this dissertation research was to determine the tick-borne pathogen composition within tick populations …

Genetic Variation And Species Distribution Of Subterranean Termites In The Southeastern U.S. Focusing On Family Structure And The Invasive Formosan Subterranean Termite, Mark Allan Janowiecki

Graduate Theses and Dissertations

This work applies molecular genetic tools to distinguish the identity and understand the biology of termites, particularly Reticulitermes Holmgren and Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae) in the southeastern U.S. Termites are important economic pests costing billions of dollars annually to Americans but also are important wood decomposers in natural settings. Molecular tools are essential for species identification because of the morphological ambiguities among species. The cryptic nest structure of subterranean termites which prevents adequate sampling makes molecular genetic tools essential to examine an entire colony.

A molecular diagnostic technique was created to differentiate Coptotermes formosanus, an invasive species in the …

Phenology And Diversity Of Arthropod Communities In Leaf Litter, Fredericka Hamilton

Graduate Theses and Dissertations

Temperate deciduous forests produce a layer of leaf litter on the forest floor annually. This layer is dynamic, and both the composition and depth change throughout the year. The leaf litter layer is an important habitat to many arthropods since they utilize it for food, shelter from adverse environmental conditions, and protection from predators.

Two commonly encountered and diverse taxa found in the litter layer are the ground beetles (Coleoptera: Carabidae) and the ground spiders (Araneae: Gnaphosidae). Carabidae and Gnaphosidae were collected and identified on a monthly basis from April 2014 to March 2015 from the Ozark Mountains of Arkansas …

The Behavioral Response Of Culex Erraticus To Different Snake Odors, Lindsey E. Wells

Honors College Theses

Eastern equine encephalitis virus (EEEV) is an arbovirus that can cause fatal infections in humans and horses. Unfortunately, the transmission mechanisms of this virus are still largely unknown. Culex erraticus displays a strong potential for serving as a vector of EEEV because of its indiscriminate feeding pattern and abundance in areas with the highest prevalence of infection. However, Culex erraticus is incapable of over-wintering the virus, yet EEE recurs each spring. Snakes may play an important role in over-wintering the virus, and certain snake species may be infected more frequently than others. This study was conducted to determine if Culex …

Movement Of Xylosandrus Germanus (Coleoptera: Curculionidae) In Ornamental Nurseries And Surrounding Habitats, Michael E. Reding, Christopher M. Ranger, Blair J. Sampson, Christopher T. Werle, Jason B. Oliver, Peter B. Schultz

Agricultural and Environmental Sciences Faculty Research

Some exotic ambrosia beetles are damaging pests in ornamental nurseries. Xylosandrus germanus (Blandford) is the most problematic ambrosia beetle in Ohio nurseries. Movement of X. germanus in nurseries has not been characterized, and knowledge is lacking on whether infestations originate from within nurseries or surrounding habitats. Flight activity of X. germanus was monitored in nurseries and adjacent wooded areas to determine the source of beetles infesting nurseries, and characterize their movement within nurseries. Ethanol-baited bottle traps were positioned within wooded areas adjacent to commercial nurseries and within nurseries at various distances from the nursery woodlot interface. Flight activity of overwintered …

Seasonal Dynamics And Micro-Climatic Preference Of Two Alpine Endemic Hypogean Beetles, Stefano Mammola, Elena Piano, Pier Mauro Giachino, Marco Isaia

International Journal of Speleology

Hypogean beetles generally live in stable environments, characterized by constant temperature and high relative humidity. Changes in the underground microclimatic conditions generally induce local migrations of the beetles through the hypogean environment in search of suitable microhabitats. We studied the seasonal dynamics and the micro-climatic preference of two Alpine endemic hypogean beetles - Sphodropsis ghilianii (Coleoptera, Carabidae) and Dellabeffaella roccae (Coleoptera, Cholevidae) - in the hypogean complex of Pugnetto (Graian Alps, Italy). We surveyed the two species for one year, using baited pitfall traps and measuring temperature and humidity along the two main caves. We used logistic regression mixed models …

In Vitro And In Vivo Evaluation Of Infestation Deterrents Against Lice, Kyong-Sup Yoon, Jennifer K. Ketzis, Samuel W. Andrewes, Christopher S. Wu, Kris Honraet, Dorien Staljanssens, Bart Rossel, J Marshall Clark

SIUE Faculty Research, Scholarship, and Creative Activity

The human head louse is a cosmopolitan ectoparasite and frequently infests many people, particularly school-age children. Due to widespread pyrethroid resistance and the lack of efficient resistance management, there has been a considerable interest in the protection of uninfested people and prevention of reinfestation by disrupting lice transfer. In this study, two nonclinical model systems (in vitro and in vivo) were used to determine the efficacy of the infestation deterrents, Elimax lotion and Elimax shampoo, against human head lice or poultry chewing lice, respectively. With in vitro assessments, female head lice exhibited significantly higher avoidance responses to hair tufts treated …

Temperature And Development Impacts On Housekeeping Gene Expression In Cowpea Aphid, Aphis Craccivora (Hemiptera: Aphidiae), Chunxiao Yang, Huipeng Pan, Yong Liu, Xuguo Zhou

Entomology Faculty Publications

Quantitative real-time PCR (qRT-PCR) is a powerful technique to quantify gene expression. To standardize gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to consistently expressed housekeeping genes (HKGs) is required. In this study, ten candidate HKGs including elongation factor 1 α (EF1A), ribosomal protein L11 (RPL11), ribosomal protein L14 (RPL14), ribosomal protein S8 (RPS8), ribosomal protein S23 (RPS23), NADH-ubiquinone oxidoreductase (NADH), vacuolar-type H+-ATPase (ATPase), heat shock protein 70 (HSP70), 18S ribosomal RNA (18S), and 12S ribosomal RNA (12S …

Colemanus Keeleyorum (Braconidae, Ichneutinae S. L.): A New Genus And Species Of Eocene Wasp From The Green River Formation Of Western North America, J. Ray Fisher, Erika Tucker, Michael Sharkey

Entomology Faculty Publications

A new genus and species of Ichneutinae s. l., Colemanus keeleyorum Fisher, is described from the Eocene Green River Formation in Colorado, USA. Colemanus was placed on a phylogenetic hypothesis using morphological data. Using a parsimony criterion, Colemanus is placed within Proteropini (Ichneutinae s. l.). Reconstructions of well-preserved regions (mesosomal dorsum and wings) are included. A previously described species from lower Oligocene Baltic amber is transferred to Colemanus, resulting in the new combination C. contortus (Brues, 1933).

The Effect Of Temperature, Relative Humidity, And Virus Infection Status On Off-Host Survival Of The Wheat Curl Mite (Acari: Eriophyidae), E. N. Wosula, A. J. Mcmechan, G. L. Hein

Department of Entomology: Faculty Publications

The wheat curl mite, Aceria tosichella Keifer, is an eriophyid pest of wheat, although its primary economic impact on wheat is due to the transmission of Wheat streak mosaic (WSMV), Wheat mosaic (also known as High Plains virus), and Triticum mosaic (TriMV) viruses. These viruses cause significant annual losses in winter wheat production throughout the western Great Plains. Temperature and humidity are factors that often influence arthropod survival, especially during dispersal from their hosts, yet the impact of these two factors on off-host survival has not been documented for wheat curl mite. Pathogen- infected host plants often influence the biology …

Eucnemid Larvae Of The Nearctic Region. Part V: Fifth Instar Larval Descriptions For Eight Species Of Microrhagus Dejean, 1833 (Coleoptera: Eucnemidae: Melasinae: Dirhagini), With Descriptions Of Four New Species And Notes On Their Biology, Robert L. Otto

Insecta Mundi

Fifth instars for eight of the nine species of Microrhagus Dejean (Coleoptera: Eucnemidae: Melasinae: Dirhagini), all endemic in the Nearctic region, are described and illustrated. Biological information is provided, along with a brief discussion of the larval morphology among the eight Nearctic species of Microrhagus. Adult descriptions are provided for four new species. These new species include Microrhagus breviangularis new species, Microrhagus carinicollis new species, Microrhagus lecontei new species and Microrhagus opacus new species. A checklist and identification keys for both adults and most known larvae are provided.

Flowers In Space Attacked By Aliens: Understanding The Spatial Ecology Behind The Devastating Damage By A Thistle Bud Weevil On Pitcher’S Thistle At Whitefish Dunes State Park, Wi, Zechariah D. Meunier

Lawrence University Honors Projects

One of the most threatened plants in the Great Lakes region is Pitcher’s thistle (Cirsium pitcheri), which inhabits sand dunes along the shorelines of Lakes Michigan, Huron, and Superior. In addition to risks from habitat loss and invasive species, C. pitcheri are imperiled by a thistle bud weevil (Larinus planus) that was originally distributed for biological control of nonnative thistles. During the summer of 2014, we empirically studied the devastating damage caused by L. planus on a population of C. pitcheri at Whitefish Dunes State Park, WI, to determine what factors influence the distribution and intensity …

Insecticide Resistance In The Bed Bug Comes With A Cost, Jennifer R. Gordon, Michael F. Potter, Kenneth F. Haynes

Entomology Faculty Publications

Adaptation to new environmental stress is often associated with an alteration of one or more life history parameters. Insecticide resistant populations of insects often have reduced fitness relative to susceptible populations in insecticide free environments. Our previous work showed that three populations of bed bugs, Cimex lectularius L., evolved significantly increased levels of resistance to one product containing both β-cyfluthrin and imidacloprid insecticides with only one generation of selection, which gave us an opportunity to explore potential tradeoffs between life history parameters and resistance using susceptible and resistant strains of the same populations. Life history tables were compiled by collecting …

Nutrition Regulation Of Male Accessory Gland Growth And Maturation In Tribolium Castaneum, Jingjing Xu, Ashlee L. Anciro, Subba R. Palli

Entomology Faculty Publications

Insulin/IGF-1 signaling (IIS) pathway is known to control growth, development and reproduction. Insulin-like peptide mediated body size plasticity in Drosophila melanogaster has been reported. Here, our studies showed that IIS pathway and nutrition regulate growth and maturation of the male accessory gland (MAG) in the red flour beetle, Tribolium castaneum. The size of MAG increased from day 1 to day 5 post-adult emergence (PAE). This increase in the size of MAG is contributed by an increase in cell size, but not cell number. The growth of MAG was impaired after double-stranded RNA (dsRNA)-mediated knockdown in the expression of genes …

Seasonal Flight Activity And Distribution Of Metallic Woodboring Beetles (Coleoptera: Buprestidae) Collected In North Carolina And Tennessee, William E. Klingeman, Jason A. Hansen, Joshua P. Basham, Jason B. Oliver, Nadeer N. Youssef, Whitney Swink, Christine A. Nalepa, Donna C. Fare, J. Kevin Moulton

Agricultural and Environmental Sciences Faculty Research

Distribution records and seasonal flight activity information for metallic woodboring beetle (Coleoptera: Buprestidae) species have not been compiled for North Carolina and Tennessee. Institutional, research, and private collections in North Carolina and Tennessee were reviewed to provide seasonal activity data of 5 subfamilies of buprestid beetle species. Label information was checked for 15,217 specimens of 135 species collected between 1901 and 2013 (North Carolina) and between 1934 and 2013 (Tennessee). These collections provided data on adult seasonal activity and county records for 121 species (4,467 specimens) and 105 species (10,750 specimens) from North Carolina and Tennessee, respectively. Two species, Agrilus …

Nebline, June 2015

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: After-Harvest Care Of Asparagus

Food & Health

Family Living

Farm & Acreage

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Time-Frequency Analysis Reveals Pairwise Interactions In Insect Swarms, James G. Puckett, Rui Ni, Nicholas T. Ouellette

Physics and Astronomy Faculty Publications

The macroscopic emergent behavior of social animal groups is a classic example of dynamical self-organization, and is thought to arise from the local interactions between individuals. Determining these interactions from empirical data sets of real animal groups, however, is challenging. Using multicamera imaging and tracking, we studied the motion of individual flying midges in laboratory mating swarms. By performing a time-frequency analysis of the midge trajectories, we show that the midge behavior can be segmented into two distinct modes: one that is independent and composed of low-frequency maneuvers, and one that consists of higher-frequency nearly harmonic oscillations conducted in synchrony …

Effects Of Minimum Winter Temperatures On Southern Pine Beetle (Dendroctonus Frontalis) Mortality On Long Island, N.Y., Jessica Cancelliere

Department of Entomology: Distance Master of Science Projects

Dendroctonus frontalis (the southern pine beetle) was discovered on Long Island, New York in 2014. Additional infestations have since been located in upstate New York, Rhode Island, Connecticut and Massachusetts. Previously found as far north as New Jersey and Pennsylvania, this range expansion now represents the current northern distribution limits of D. frontalis. Minimum winter temperatures are a meaningful driver of population dynamics and mortality in D. frontalis, and understanding this relationship at the northern range limits can help forest managers better predict and prepare for outbreaks. Studies addressing the relationship between temperature and beetle mortality, and the …

Selection Of Reference Genes For Rt-Qpcr Analysis In The Monarch Butterfly, Danaus Plexippus (L.), A Migrating Bio-Indicator, Huipeng Pan, Xiaowei Yang, Keith Bidne, Richard L. Hellmich, Blair D. Siegfried, Xuguo Zhou

Entomology Faculty Publications

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a powerful technique to quantify gene expression. To facilitate gene expression study and obtain accurate results, normalization relative to stably expressed reference genes is crucial. The monarch butterfly, Danaus plexippus (L.), is one of the most recognized insect species for its spectacular annual migration across North America. Besides its great voyages, D. plexippus has drawn attention to its role as a bio-indicator, ranging from genetically modified organisms (GMOs) to natural ecosystems. In this study, nine reference genes from D. plexippus genome were selected as the candidate reference genes. The expression profiles of these …

Exceptionally High Levels Of Genetic Diversity In Wheat Curl Mite (Acari: Eriophyidae) Populations From Turkey, W. Szydło, Gary L. Hein, E. Denizhan, A. Skoracka

Department of Entomology: Faculty Publications

Recent research on the wheat curl mite species complex has revealed extensive genetic diversity that has distinguished several genetic lineages infesting bread wheat (Triticum aestivum L.) and other cereals worldwide. Turkey is the historical region of wheat and barley (Hordeum vulgare L.) domestication and diversification. The close relationship between these grasses and the wheat curl mite provoked the question of the genetic diversity of the wheat curl mite in this region. The scope of the study was to investigate genetic differentiation within the wheat curl mite species complex on grasses in Turkey. Twenty-one wheat curl mite populations from …