Entomology Commons^™

Revision And Reclassification Of The Genera Of Phalacridae (Coleoptera: Cucujoidea), Matthew Gimmel

LSU Doctoral Dissertations

The family Phalacridae is monographed at the genus level using modern phylogenetic and classification methods. A morphological matrix of 106 characters and 2063 base pairs of aligned 18S rDNA sequence data were analyzed both separately and in a concatenated matrix, using maximum parsimony, Bayesian methods, and maximum likelihood. The phylogenetic hypotheses were used to construct a new subfamilial classification for the family. I recognize eight subfamilies, including two described as new: Biophytinae Guillebeau 1894, Eustilbinae Guillebeau 1892, Ochrolitinae Guillebeau 1894, Olibrinae Guillebeau 1892, Olibroporinae, subfam. nov., Olibrosominae, subfam. nov., Phaenocephalinae Matthews 1899, Phalacrinae Guillebeau 1892. Twenty-eight new generic synonymies are …

Molecular Mechanisms Of Bacillus Thuringiensis Resistance In The Sugarcane Borer, Yunlong Yang

LSU Doctoral Dissertations

The sugarcane borer, Diatraea saccharalis, is a major target pest of transgenic corn expressing Bacillus thuringiensis (Bt) proteins in many areas of the U.S. mid-southern region. A Cry1Ab-resistant (Cry1Ab-RR) strain of D. saccharalis has been developed from a single two-parent family-line. To examine the molecular mechanisms of the Cry1Ab resistance in this insect strain, cDNAs of five types of potential candidate genes related to Bt resistance were sequenced using reverse transcriptase polymerase chain reaction (RT-PCR) and 5’ rapid amplification of cDNA end (5’ RACE). The Bt resistance candidate genes examined included three trypsins (DsTRYs), three chymotrypsins (DsCHYs), three aminopeptidases N …

Biological Control Of The Black Cutworm, Agrotis Ipsilon (Lepidoptera: Noctuidae), And Endophyte Mediated Tritrophic Interactions In Turfgrass, Andrea Jeanne Bixby-Brosi

University of Kentucky Doctoral Dissertations

Components of successful pest management programs must be complementary and not antagonistic. This project examined interactions between natural enemies of the black cutworm, Agrotis ipsilon (Hufnagel), an important turfgrass pest, and host plant resistance by endophytic grass.

Agrotis ipsilon nucleopolyhedrovirus (AgipMNPV) was examined as a bio-insecticide for controlling A. ipsilon in turfgrass. Fresh (1-week-old) AgipMNPV residues killed 76−86% of neonates hatching from eggs on golf course tees, however, residual control of implanted larvae lasted no more than a few weeks. Combinations of AgipMNPV with adjuvants, such as optical brightener and lignin, failed to accelerate or extend …

Ixodes Affinis (Acari: Ixodidae) In Southeastern Virginia And Implications For The Spread Of Borrelia Burgdorferi, The Agent Of Lyme Disease, Robyn M. Nadolny, Chelsea L. Wright, Wayne L. Hynes, Daniel E. Sonenshine, Holly Gaff

Biological Sciences Faculty Publications

Ixodes affinis Neumann is a hard-bodied (ixodid) tick known to be a competent vector for Borrelia burgdorferi, the agent of Lyme disease, and agents of other human diseases (Keirans et al. 1999). Ixodes affinis has been reported in Florida, Georgia, and South Carolina and throughout coastal North Carolina (Clark et al. 1998, Harrison et al. 2010). Harrison et al. (2010) indicated that I. affinis was established throughout the coastal plain of North Carolina up to the Virginia border and suggested that I. affinis might occur in Virginia.

Phylogeographic Structure Of Teretrius Nigrescens (Coleoptera: Histeridae) Predator Of The Invasive Post Harvest Pest Prostephanus Truncatus (Coleoptera: Bostrichidae)., B. A. Omondi, J. Van Den Berg, D. Masiga, F. Schulthess

All Peer-Reviewed Publications

The invasive larger grain borer Prostephanus truncatus (Horn) is the most important pest of farm-stored maize in Africa. It was introduced into the continent from Mesoamerica in the late 1970s and by 2008 had spread to at least 18 countries. Classical biological control using two populations of the predator Teretrius nigrescens Lewis achieved long-term and cost effective control in warm-humid areas, but not in cool and hot-dry zones. The present study investigated the phylogenetic relationships between geographical populations of the predator. Ten populations of T. nigrescens were studied using randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR), sequence analysis of …

Parasitism Of Lepidopterous Stem Borers In Cultivated And Natural Habitats, Duna Madu Mailafiya, Bruno Pierre Le Ru, Eunice Waitherero Kairu, Stéphane Dupas, Paul-André Calatayud

All Peer-Reviewed Publications

Plant infestation, stem borer density, parasitism, and parasitoid abundance were assessed during two years in two host plants, Zea mays (L.) (Cyperales: Poaceae) and Sorghum bicolor (L.) (Cyperales: Poaceae), in cultivated habitats. The four major host plants (Cyperus spp., Panicum spp., Pennisetum spp., and Sorghum spp.) found in natural habitats were also assessed, and both the cultivated and natural habitat species occurred in four agroecological zones in Kenya. Across habitats, plant infestation (23.2%), stem borer density (2.2 per plant), and larval parasitism (15.0%) were highest in maize in cultivated habitats. Pupal parasitism was not higher than 4.7% in both habitats, …

Dissemination Of Entomopathogenic Fungi Using Busseola Fusca Male As Vector, Nguya K. Maniania, Elizabeth Ouna, Peter Ahuya, Brigitte Frérot, Anne Emmanuelle Félix, Bruno Le Ru, Paul André Calatayud

All Peer-Reviewed Publications

The stem borer, Busseola fusca (Fuller), is an important pest of maize Zea mays L. and sorghum Sorghum bicolor (L.) in eastern and southern Africa. To control this pest, biological control methods including the use of entomopathogenic fungi are being considered. The pathogenicity of one isolate of Metarhizium anisopliae (Metsch.) Sorok. and one isolate of Beauveria bassiana Bals. (Vuill.) were first tested on different developmental stages of B. fusca including eggs, neonate, 2nd and 3rd-instar larvae. Both fungal isolates were pathogenic to all the stages tested. However, differences in mortality were observed among larvae that hatched from treated egg masses. …

Sex Pheromone Composition And Reproductive Isolation In Two Busseola Species (Lepidoptera: Noctuidae) In Kenya, A. E. Félix, P. A. Calatayud, B. Le Ru, J. F. Silvain, B. Frérot

All Peer-Reviewed Publications

Closely related species provide an interesting and useful model to understand mate communication diversification. Pre-mating isolation is the result of selection processes and is achieved by utilization of different sex pheromones, but also by different ecological and life traits such as geographic distribution, host-plant specialization and allochrony of sexual behavior. Here, we report sex pheromone identifications and pre-mating isolation of two closely related species of stemborers: Busseola segeta and the yet undescribed Busseola nov. sp., developing on different host-plants and in different geographical locations in Kenya. GC-MS analyses showed that the two species produced the same pheromone components, but in …

Oviposition Preferences Of The African Wild Silkmoth, Gonometa Postica Walker (Lepidoptera: Lasiocampidae) On Different Substrates, Ken O. Fening, Esther N. Kioko, Jones M. Mueke, Suresh K. Raina

All Peer-Reviewed Publications

Gonometa postica Walker (Lepidoptera: Lasiocampidae) is currently being utilised for commercial wild silk production in Eastern Kenya. The oviposition preferences of female G. postica on four substrates in a net-sleeved cage was studied in the laboratory in the long and short rainy seasons in 2007 for two generations, i. e. early (late March to April) and late flights (late September to October) of the moth. The first generation moths laid the highest number of eggs on net sleeves followed by plastic pot, twigs and wooden plank, whereas the second generation moths preferred wooden plank and net sleeves over the plastic …

The Flight Activity Of Prostephanus Truncatus (Horn) (Coleoptera: Bostrichidae) And Teretrius Nigrescens Lewis (Coleoptera: Histeridae) In Kenya, Bonaventure Aman Omondi, Nanqing Jiang, Johnnie Van Den Berg, Fritz Schulthess

All Peer-Reviewed Publications

The invasive storage pest, the larger grain borer (LGB) Prostephanus truncatus (Horn), was introduced into the maize-deficit, semi-arid areas of the eastern region in Kenya in the early 1980s. In spite of containment efforts and the introduction of the predator Teretrius nigrescens Lewis from Mexico, LGB has spread to the main maize production zone in western Kenya during the past five years. The present work presents results from a 28-month monitoring effort of LGB and its predator, using pheromone traps at five locations along an east-west transect across Kenya. LGB occurred in all regions with highest trap catches in the …

Repellent Activity Of Catmint, Nepeta Cataria, And Iridoid Nepetalactone Isomers Against Afro-Tropical Mosquitoes, Ixodid Ticks And Red Poultry Mites, Michael A. Birkett, Ahmed Hassanali, Solveig Hoglund, Jan Pettersson, John A. Pickett

All Peer-Reviewed Publications

The repellent activity of the essential oil of the catmint plant, Nepeta cataria (Lamiaceae), and the main iridoid compounds (4aS,7S,7aR) and (4aS,7S,7aS)-nepetalactone, was assessed against (i) major Afro-tropical pathogen vector mosquitoes, i.e. The malaria mosquito, Anopheles gambiae s.s. and the Southern house mosquito, Culex quinquefasciatus, using a World Health Organisation (WHO)-approved topical application bioassay (ii) the brown ear tick, Rhipicephalus appendiculatus, using a climbing repellency assay, and (iii) the red poultry mite, Dermanyssus gallinae, using field trapping experiments. Gas chromatography (GC) and coupled GC-mass spectrometry (GC-MS) analysis of two N. cataria chemotypes (A and B) used in the repellency assays …

Introduction And Establishment Of Cyrtobagous Salviniae Calder And Sands (Coleoptera: Curculionidae) For The Control Of Salvinia Minima Baker (Salviniaceae), And Interspecies Interactions Possibly Limiting Successful Control In Louisiana, Katherine A. Parys

LSU Doctoral Dissertations

Common salvinia, Salvinia minima Baker, is a floating aquatic invasive macrophyte that obstructs waterways and causes problems in wetlands across Louisiana and Texas. The salvinia weevil, Cyrtobagous salviniae Calder and Sands, has been released in over 14 countries around the world for the biological control of Salvinia spp. We successfully monitored the introduction and establishment of C. salviniae on S. minima in southern Louisiana between 2006 and 2010. Cyrtobagous salviniae significantly lowered the biomass of S. minima and increased the number of terminal buds damaged but had no significant impact on pH, dissolved oxygen, conductivity, surface temperature, percent of surface …

Agroecological Factors Impacting Stem Borer (Lepidoptera: Crambidae) Dynamics In Gulf Coast Sugarcane And Rice, Julien M. Beuzelin

LSU Doctoral Dissertations

Diatraea saccharalis (F.) and Eoreuma loftini (Dyar) are stem boring pests of sugarcane (Saccharum spp.) and rice (Oryza sativa L.) crops in the Gulf Coast region. Studies were conducted to determine the role of agroecological factors, including predator disruptions, alternate hosts, and crop phenological conditions, on stem borer populations.
The year after Hurricane Rita storm surge flooded sugarcane in Louisiana, a 71% reduction in the predaceous Solenopsis invicta Buren was recorded. Even with a 2.4-fold increase in the number of insecticide applications used for D. saccharalis management in flooded fields, growers still incurred higher injury.
In two field …

Contribution Of Bacillus Thuringiensis Cotton Cultivars And Insecticides To A Fall Armyworm, Spodoptera Frugiperda (J. E. Smith), (Lepidoptera : Noctuidae) Management Strategy, Jarrod T. Hardke

LSU Doctoral Dissertations

Fall armyworm, Spodoptera frugiperda (J. E. Smith), response to cottons, Gossypium hirsutum L., expressing Bacillus thuringiensis (Bt) insecticidal proteins, Cry1Ac (Bollgard®), Cry1Ac + Cry2Ab (Bollgard II®), and Cry1Ac + Cry1F (WideStrike™), was evaluated in field and laboratory experiments. In field trials, larvae that were infested on selected fruiting forms (squares, white flowers, and bolls) of WideStrike™ plants had lower survivorship and caused less injury than larvae on non-Bt plants, regardless of fruiting structures. Bollgard® and Bollgard II® plants produced no consistent negative effects on fall armyworm survivorship and injury. In no-choice laboratory assays, Bollgard II® and WideStrike™ cotton tissue reduced …

Role Of Substrate Moisture, Relative Humidity And Temperature On Survival And Foraging Behavior Of Formosan Subterranean Termites, Bal Krishna Gautam

LSU Doctoral Dissertations

The Formosan subterranean termite is an important structural pest in the southern United States, especially Louisiana. Baiting systems and spot treatment with non-repellent delayed action termiticides are among the most eco-friendly control approaches. However, to achieve a desired success from these approaches requires a sophisticated knowledge of foraging behavior and food transfer system of these social insects. Moisture and temperature play a vital role in influencing the foraging behavior of these desiccation prone insects. A series of laboratory experiments were conducted to understand the subtleties of foraging behavior of Coptotermes formosanus Shiraki in relation to moisture, relative humidity and temperature. …

Advanced Management Of The Mexican Rice Borer (Eoreuma Loftini) In Sugarcane, Blake Emerson Wilson

LSU Master's Theses

Insecticide, greenhouse and varietal resistance experiments were conducted in Texas to develop management tactics for the Mexican rice borer, Eoreuma loftini (Dyar), sugarcane. A 3-treatment, large plot aerial application study was set up in 5 commercial sugarcane fields (35-84 acres) to evaluate the utilization of pheromone traps to improve chemical control strategies for E. loftini during 2009 and 2010 growing seasons. A threshold of 20-25 moths/trap/wk was used as an indicator to initiate monitoring for E. loftini larval infestations. Larval infestations were directly related to the no. of moths/trap/wk (R2=0.71). Reductions in borer injury and adult emergence (60% and 40% …

Ecology And Systematics Of Coleoptera In Woody Debris Of Eastern North American Forests, Michael Leslie Ferro

LSU Doctoral Dissertations

Dead wood is a largely unexplored reservoir of taxonomic diversity and its ecology is poorly known despite its use as fuel and its roles in the carbon cycle and healthy ecosystems. During this research 15 new species of dead wood associated rove beetles (Staphylinidae: Pselaphinae) in the genus Sonoma Casey were discovered and described. Sonoma tolulae (LeConte) was redescribed. Taxonomic issues obfuscated the identity of another dead wood associate, Thoracophorus costalis (Erichson) (Staphylinidae: Osoriinae). Examination of holotypes resulted in the discovery of two nomenclatural synonyms: T. longicollis Motschulsky, and T. fletcheri Wendeler. An inexpensive emergence chamber used to collect insects …

A Study Of Horn Fly, Haematobia Irritans (L.) (Diptera: Muscidae), Target-Site Sensitivity, Susceptibility, And Resistance Management At Selected Sites In Louisiana, Cole David Younger

LSU Doctoral Dissertations

The purpose of the first study was to evaluate the effectiveness of the use of a mid-season avermectin pour-on to cattle for managing OP resistance in horn fly populations at the Rosepine Louisiana Agricultural Research Station. During an eight year study, ivermectin treatments were made three times (when the treatment threshold of less than five weeks of control was reached), and in the following years, the number of weeks of control increased to 9 to 10 weeks each time. The purpose of the second study was to test a two-year OP/one-year pyrethroid rotation strategy as a strategy for maintaining susceptibility …

Lack Of Rhythmicity In The Honey Bee Queen: An Investigation Of Temporal Behavioral Patterns In Apis Mellifera Ligustica., Jennifer N. Johnson

Electronic Theses and Dissertations

Little is known about the behavioral patterns of honey bee queens. To determine if mated honey bee queens possess diel rhythmicity in behavior, we observed them in glass-sided observation hives using three types of observation regimes: focal studies consisting of 2-hour and 24-hour continuous observations as well as scan-sampling of multiple queens. All behaviors (active: walking, inspecting, egg-laying, begging for food, feeding, and grooming self; inactive: standing) occurred at all times of day and night, but no queen showed consistent diel rhythmicity in any of the individual behaviors. There were no consistent diel differences in active versus inactive behaviors or …

A Re-Examination Of Diplectrona Modesta Banks 1908 (Trichoptera: Hydropsychidae) Using Morphological And Molecular Techniques, Lauren Harvey

All Theses

Diplectrona modesta Banks 1908 (Trichoptera: Hydropsychidae) is a common inhabitant of streams across eastern North America. This species is well known and used often in biomonitoring studies. Experts in the field have long noted the morphological variation in larval head color and patterns as well as the species presence in many different types of habitats, such as springs, seeps, streams, and some small rivers. This study examines the species both morphologically and molecularly using mitochondrial and nuclear genes in order to determine if the species is a complex of several species or one highly variable species. DNA sequencing using the …