Biotechnology Commons^™

Effect Of Bioaugmentation Product Biowish® Aqua™ On Nitrogen Removal In Wastewater, Patrick Cassidy Kalvass

Master's Theses

Biological nutrient removal (BNR) from wastewater, and specifically nitrogen removal, is a growing concern to wastewater dischargers such as municipalities. Excess nutrients in effluent can create problems such as eutrophication, toxicity to aquatic life, and dissolved oxygen depletion in receiving waters. BNR systems have been installed in many locations with success, but their operation presents operational and financial demands greater than conventional biological treatment. Nitrogen removal is typically performed in sequential autotrophic nitrification and denitrification, which increases needed energy input, operational complexity, and therefore cost. Simultaneous nitrification-denitrification (SNdN) achieved in a single system has also been successfully implemented, however operational …

Mechanism Of Virus Inactivation By Cold Atmospheric-Pressure Plasma And Plasma-Activated Water, Li Guo, Ruobing Xu, Lu Gou, Zhichao Liu, Yiming Zhao, Dingxin Liu, Lei Zhang, Hailan Chen, Michael G. Kong

Bioelectrics Publications

ABSTRACT Viruses cause serious pathogenic contamination that severely affects the environment and human health. Cold atmospheric-pressure plasma efficiently inactivates pathogenic bacteria; however, the mechanism of virus inactivation by plasma is not fully understood. In this study, surface plasma in argon mixed with 1% air and plasma-activated water was used to treat water containing bacteriophages. Both agents efficiently inactivated bacteriophages T4, ϕ174, and MS2 in a time-dependent manner. Prolonged storage had marginal effects on the antiviral activity of plasma-activated water. DNA and protein analysis revealed that the reactive species generated by plasma damaged both nucleic acids and proteins, consistent with the …

Exploiting Click-Chemistry And Microfluidics To Map The Neuronal Itinerary Of App Processing And Amyloid-Beta Generation, Namratha Srinivas

McKelvey School of Engineering Graduate Student Theses & Dissertations

Alzheimer’s disease (AD) is a chronic neurodegenerative disease and is the sixth leading cause of death in the United States with approximately 5.5 million Americans diagnosed with it. The neuropathological hallmark includes extracellular senile plaques and intraneuronal neurofibrillary tangles. Recent GWAS studies have identified genes associated with AD, and have revealed several classes of genes implicated in disease pathogenesis. In particular, three general pathways associated with an increased risk of AD included: 1) cholesterol metabolism, innate immune system, and the membrane trafficking. Our lab has focused on intracellular trafficking as it relates to the processing of amyloid precursor protein (APP), …

The Effect Of Compound L19 On Human Colorectal Cells (Dld-1), Sepideh Mohammadhosseinpour

Electronic Theses and Dissertations

Colorectal cancer is the third most common type of cancer in the world and the second leading cause of death among humans. Extracts of water soluble compounds from the roots and leaves of Rumex crispus were screened for compounds that induced apoptosis in DLD-1 cells. A compound referred to as L19 was isolated using Accelerated Solvent Extraction (ASE) followed by High Performance Liquid Chromatography (HPLC). Gas chromatography coupled with mass spectrometry was used to identify the L19 as a tetrahydrofuran with a molecular weight of 72.11 g/mole. Each HPLC fraction resulted in 94.4% purity. In the present research, specific genes …

Same-Sex Marriage And The Baby Carriage: A Post-Obergefell Analysis Of Art Funding For Same-Sex Couples In The United States, Charmaine Mech

Georgia Journal of International & Comparative Law

No abstract provided.

Identifying A B. Thuringiensis Var. Kurstaki Receptor Binding Protein For Bacteriophage Riley, Rachel Carson

Senior Honors Projects, 2010-2019

With the rapid emergence of antibiotic resistant bacteria affecting people around the world, research into new therapies using bacteriophages (phages) is increasing in the United States. Phages are viruses that can only infect bacteria and are able to co-evolve alongside the bacteria they infect. A researchers’ ability to pinpoint which phage to use in the therapy is important to combat an infection effectively. To do so, the genes that control the interaction between phages and the bacteria they infect, such as receptor binding proteins on the surface of a bacterial cell, need to be identified. Transposon mutagenesis was used in …

Evolution Of Bordetella Pertussis Genome May Play A Role In The Increased Rate Of Whooping Cough Cases In The United States, Kevin Loftus

Senior Honors Projects, 2010-2019

Bordetella pertussis is the bacterium responsible for pertussis, a disease commonly referred to as whooping cough. Recently, pertussis has made a resurgence in the U.S. despite high-vaccination coverage. Possible causes of the increased number of pertussis cases include genetic evolution of B. pertussis, increased awareness of the disease, better laboratory diagnostics, and the switch from a whole-cellular (wP) vaccine to an acellular vaccine (aP) in the 1990s. Fortunately, just as B. pertussis is evolving, so is the arsenal of technologies used to understand and combat this pathogenic bacterium. Whole genome sequencing is one technology that helps researchers better understand …

Assessment Of Resistance To Botrytis Cinerea In Arabidopsis Expressing Grapevine Sts Genes And Analysis Of New Grapevine Vein Clearing Virus Isolates, Li Su

Graduate Theses/Dissertations

Grapevine stilbene synthase gene (STS) family is unusually large with more than 32 members of full-length genes. Resveratrol and piceid are synthesized in VaSTS7-, VvSTS22-, VaSTS22-transgenic Arabidopsis. To study the function of these STS genes in the resistance against Botrytis cinerea in vivo, lesion sizes on these STS transgenic Arabidopsis were compared to that on non-transgenic control Col-0, at 96 hours post inoculation with B. cinerea spores at 0, 200, or 1,000 spores/μl. In each experiment there were three biological replicates. Three lesion sizes from each replicate were averaged. The experiment was repeated three …

Optimizing Genetic Manipulation Of Methanogens Through Faster Cloning Techniques, Merrisa Jennings

Biological and Agricultural Engineering Undergraduate Honors Theses

Methanogenesis is the biological production of methane. Only anaerobic archaea known as methanogens are capable of such a metabolic feat. They have strict living conditions and substrate sources which determine their rate of metabolism. This is of particular importance from a greenhouse gas reduction perspective or biogas capturing perspective. One of the best ways to optimize methanogen methane production is via genetic manipulation. The current procedures are timely though, therefore a faster cloning processes should be developed. The objective of this study was to optimize a premade genetic transformation kit known as the Gibson Kit. The Gibson Kit was supposed …

Ketone Bodies And Signaling In Pancreatic Cancer Cell Lines, Kyla B. Buettner, Pankaj K. Singh, Surendra K. Shukla

Theses/Capstones/Creative Projects

Pancreatic cancer is the fourth leading cause of cancer-related deaths in the United States, and 95% of these cases are caused by PDAC (pancreatic ductal adenocarcinoma). Ketone bodies have previously been shown to decrease cell proliferation and cancer-induced cachexia. The molecular mechanism of ketone body-mediated growth inhibition of pancreatic cancer cells is not well understood. Research conducted thus far has not explored which molecular pathways are affected by ketone body treatment in pancreatic cancer cells. In the current study, the effect of the ketone body sodium hydroxybutyrate on the JAK-STAT and mTOR pathways and cell migration was explored. A decrease …

Optimization Of Recombinant Adeno-Associated Virus (Aav) Vector Production In Saccharomyces Cerevisiae, Juan Aponte

KGI Theses and Dissertations

Recombinant adeno-associated viral vectors (rAAV) are emerging drugs for gene therapy applications. Their non-pathogenic status, low inflammatory potential, availability of viral serotypes with different tissue tropisms, and prospective long-lasting gene expression are important attributes that make rAAVs safe and efficient therapeutic options. One of the main limitations for bringing rAAV gene therapy to the market is the difficulty in supplying enough rAAV vector product. The high vector doses suggested by early clinical data infer the need to scale up production at high volumes in order to satisfy patient demand. Current production platforms such as HEK293 or Sf9 cells are very …

Reversible Heparin Molecules And Methods Of Making And Using The Same, Jian Liu, Yongmei Xu, Robert J. Linhardt, Edward Harris

Department of Biochemistry: Faculty Publications

Methods and systems for synthesizing heparin compounds are provided . The chemoenzymatic synthesis of structurally homogeneous low molecular weight heparins that have a reversible anticoagulant activity is provided . Also disclosed are heparin compounds having anticoagulant activity , including a binding affinity to antithrombin and an anti - Xa activity , but no detectable anti - lla activity . Additionally , provided are synthetic , low - molecular weight heparin com pounds with reversible anticoagulant activity , where the anticoagulant activity is reversible by protamine.

Restoration Of Phage Growth On A Non-Permissive Host By Bypassing Transcription Termination Signals, Millicent Ronkainen

Mahurin Honors College Capstone Experience/Thesis Projects

RNA polymerase is the central enzyme in all gene expression. The rpoCY75N mutation in the zinc-binding domain of the β’ subunit of E. coli RNA polymerase blocks a unique RNA-based mechanism of transcription antitermination utilized by bacteriophage HK022 and its relatives. Here, we describe the characterization of mutant phage, orc0368, which overcomes the rpoCY75N mutation. The orc0368 genome varies from the wild type phage genome by 4 single base pair mutations. Three of these mutations were not characterized because they occur in intergenic regions but the fourth was chosen for study because of its location between a series …

Algal Biofuel: The Future Of Green Jet Fuel In Air Transportation, Rajee Olaganathan

Publications

The aviation industry is one of the major contributors for the greenhouse gases. As air travel has become inevitable in this modern era, and fossil fuel usage is not sustainable, it is essential to produce renewable fuel and commercialize it to reduce the greenhouse gas emissions. In order to produce an alternate aviation biofuel a lot of industrial and academic collaborations have been developed worldwide. The main concern of this collaborative research is to produce aviation biofuel from renewable resources with low environmental impacts, and which is sustainable at an economically viable price. This mini-review briefly discusses the biotechnological approaches …

Keragaan Tiga Jenis Planlet Anggrek Phalaenopsis Asal Protocorm Yang Diinduksi Ethyl Methyl Sulfonate (Ems) Secara In Vitro, Romiyadi, Ai Komariah, Suseno Amien

Jurnal Kultivasi

This research was conducted to determine the effect of concentration of Ethyl Methyl Sulfonate (EMS) to performance of three types of Phalaenopsis Planlets and to know the optimum concentration of EMS on any type of Phalaenopsis orchids. The experiment was conduc-ted at Tissue Culture Laboratory of the Faculty of Agriculture, Winaya Mukti University, Sumedang. It used a completely randomized design (CRD) with factorial pattern, that consisted of two factors and replicated twice.The first factor was the type of Phalaenopsis that resulted form hybridization which consisted of v1 (Phalaenopsis 717 X Phalae-nopsis Fire Bird), v2 (Phalaenopsis Tianong Rose X Sibling), and …

Effect Of Vitrification On Epigenetic Modifications And The Meiotic Spindle Of Bovine Oocytes, Emilio Jose Gutierrez-Castillo

LSU Master's Theses

While oocyte vitrification has become a common practice, it still faces some challenges such as the low survival rates after warming, probably related to cryoinjuries and cryoprotectant (CPA) toxicity. Evidence suggests that vitrification might have an effect on the patterns of some epigenetic marks including DNA methylation and histone acetylation. During fertilization and embryogenesis, key events for healthy and adequate embryo development take place, not only governed by the information contained within the DNA sequence, but also by epigenetic mechanisms.

This study was aimed at determining the effect of vitrification and CPA exposure, using a combination of ethylene glycol (EG) …

Intellectual Property, Surrogate Licensing, And Precision Medicine, Jacob S. Sherkow, Jorge L. Contreras

IP Theory

The fruits of the biotechnology revolution are beginning to be harvested. Recent regulatory approvals of a variety of advanced therapies—Keytruda (pembrolizumab), Kymriah (tisagenlecleucel), and patisiran—have ushered in an age of “precision medicine” treatments that target patients’ specific genetic, physiological, and environmental profiles rather than generalized diagnoses of disease. Therapies like these may soon be supplemented by gene editing technologies such as CRISPR, which could enable the targeted eradication of deleterious genetic variants to improve human health. But the intellectual property (IP) surrounding precision therapies and their foundational technology remain controversial. Precision therapies ultimately rely—and are roughly congruent with—basic scientific information …

Thermal Efficient Design Of Distributed Memorgenerator For Dual-Port Ram Using Unidirectional High-Performance Io Standard, Das B., Abdullah M.F.L.

ASEAN Journal on Science and Technology for Development

he distributeddual-port RAM offersthe high-speed data rate transmission for differentmemory access modes such as: busy mode; interrupt mode; JTAG mode; maser mode; slave mode; and sleep mode, at high-frequency operation. The execution of these modes at high-frequency operation increases the on-chip temperature of distributeddual-port RAM. It might short the distribute dual-port RAM forever. Currently, differenttechniques have been reported, but significanton-chip temperature consumption is not reduced for distributeddual-port RAM. In this paper, the thermal-efficiendesign for disributed dual-port RAM was achieved using IO sandard technique. The distributeddual-port RAM was designed using differentIO standardssuch as; LVTTL IO standardand Mobile_DDR IO standard.It was determined …

Ediorial Board, Board Editorial

ASEAN Journal on Science and Technology for Development

No abstract provided.

Influence Of Tespt On Tensile And Tear Strengths Of Vulcanized Silica-Filled Natural Rubbe, Norizah A.K., Azemi S.

ASEAN Journal on Science and Technology for Development

The effectof coupling agent on tensile and tear strengthsof vulcanized NR filledwith 50 pphr of precipitated silica (VN3) was investgated. The amount of coupling agent was varied from 0,1,2,3,4,5 to 8 pphr. In the absence of coupling agent bis[3-(triethoxysilyl)propyl] tetrasulphide (TESPT), the tensile strengthof silica-fille vulcanized NR was lower than the tensile strengthof unfilledvulcanized NR. The enhancement in the tensile strengthwas achieved only when TESPT was incorporated into the rubber compound. The dependence of tensile strengthon the amount of TESPT showed a similar trend as the dependence of tensile strengthon the crosslink concentration. This might imply that varying the amount …